Origin IGS: tttctaaacgatccgaagtgtcggatatggttaaatcatagaggtgattttcaaatcatttactttttatagaaggggctgaccggttttgacagcgggcagaagtggtttgtaagcatgcagtgcgtcgttggccggcactttaatctcggttgatccaattttaactggcgaaaataactacgctctcgctgcttaatcgaagcacagtagattgaagcttaatccttacacaaggtgtttggacgagacatcacccggaagccgtggcttcgaggcaatccgaaccggtggtgcagaaatgtcggagatagcttgacggatgtcccgtacggcaagtgaaaattcagggaataaggttgcggtgggtggcttcggtcttgccgcagcacgaaaatgaaggcgaagataagcatgtagaaagcaaattagttcctcgtttggacgagagttcgaatctctccagctccacatttcgggcgatca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tgatcgcccgaaatgtggagctggagagattcgaactctcgtccaaacgaggaactaatttgctttctacatgcttatcttcgccttcattttcgtgctgcggcaagaccgaagccacccaccgcaaccttattccctgaattttcacttgccgtacgggacatccgtcaagctatctccgacatttctgcaccaccggttcggattgcctcgaagccacggcttccgggtgatgtctcgtccaaacaccttgtgtaaggattaagcttcaatctactgtgcttcgattaagcagcgagagcgtagttattttcgccagttaaaattggatcaaccgagattaaagtgccggccaacgacgcactgcatgcttacaaaccacttctgcccgctgtcaaaaccggtcagccccttctataaaaagtaaatgatttgaaaatcacctctatgatttaaccatatccgacacttcggatcgtttagaaa Mask Tandem Repeat Region ================================================ tttctaaacgatccgaagtgtcggatatggttaaatcatagaggtgattttcaaatcatttactttttatagaaggggctgaccggttttgacagcgggcagaagtggtttgtaagcatgcagtgcgtcgttggccggcactttaatctcggttgatccaattttaactggcgaaaataactacgctctcgctgcttaatcgaagcacagtagattgaagcttaatccttacacaaggtgtttggacgagacatcacccggaagccgtggcttcgaggcaatccgaaccggtggtgcagaaatgtcggagatagcttgacggatgtcccgtacggcaagtgaaaattcagggaataaggttgcggtgggtggcttcggtcttgccgcagcacgaaaatgaaggcgaagataagcatgtagaaagcaaattagttcctcgtttggacgagagttcgaatctctccagctccacatttcgggcgatca Find is-nt database================================================ Query_seq: TF0527:TF0528|TF0527:TF0528:prolyl endopeptidase:two-component system sensor histidine kinase:->->:552011..552496 486 tttctaaacgatccgaagtgtcggatatggttaaatcatagaggtgattttcaaatcatttactttttatagaaggggctgaccggttttgacagcgggcagaagtggtttgtaagcatgcagtgcgtcgttggccggcactttaatctcggttgatccaattttaactggcgaaaataactacgctctcgctgcttaatcgaagcacagtagattgaagcttaatccttacacaaggtgtttggacgagacatcacccggaagccgtggcttcgaggcaatccgaaccggtggtgcagaaatgtcggagatagcttgacggatgtcccgtacggcaagtgaaaattcagggaataaggttgcggtgggtggcttcggtcttgccgcagcacgaaaatgaaggcgaagataagcatgtagaaagcaaattagttcctcgtttggacgagagttcgaatctctccagctccacatttcgggcgatca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF0527:TF0528|TF0527:TF0528:prolyl endopeptidase:two-component system sensor histidine kinase:->->:552011..552496 486 tttctaaacgatccgaagtgtcggatatggttaaatcatagaggtgattttcaaatcatttactttttatagaaggggctgaccggttttgacagcgggcagaagtggtttgtaagcatgcagtgcgtcgttggccggcactttaatctcggttgatccaattttaactggcgaaaataactacgctctcgctgcttaatcgaagcacagtagattgaagcttaatccttacacaaggtgtttggacgagacatcacccggaagccgtggcttcgaggcaatccgaaccggtggtgcagaaatgtcggagatagcttgacggatgtcccgtacggcaagtgaaaattcagggaataaggttgcggtgggtggcttcggtcttgccgcagcacgaaaatgaaggcgaagataagcatgtagaaagcaaattagttcctcgtttggacgagagttcgaatctctccagctccacatttcgggcgatca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF0527:TF0528|TF0527:TF0528:prolyl endopeptidase:two-component system sensor histidine kinase:->->:552011..552496 486 tttctaaacgatccgaagtgtcggatatggttaaatcatagaggtgattttcaaatcatttactttttatagaaggggctgaccggttttgacagcgggcagaagtggtttgtaagcatgcagtgcgtcgttggccggcactttaatctcggttgatccaattttaactggcgaaaataactacgctctcgctgcttaatcgaagcacagtagattgaagcttaatccttacacaaggtgtttggacgagacatcacccggaagccgtggcttcgaggcaatccgaaccggtggtgcagaaatgtcggagatagcttgacggatgtcccgtacggcaagtgaaaattcagggaataaggttgcggtgggtggcttcggtcttgccgcagcacgaaaatgaaggcgaagataagcatgtagaaagcaaattagttcctcgtttggacgagagttcgaatctctccagctccacatttcgggcgatca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tttctaaacgatccgaagtgtcggatatggttaaatcatagaggtgattttcaaatcatttactttttatagaaggggctgaccggttttgacagcgggcagaagtggtttgtaagcatgcagtgcgtcgttggccggcactttaatctcggttgatccaattttaactggcgaaaataactacgctctcgctgcttaatcgaagcacagtagattgaagcttaatccttacacaaggtgtttggacgagacatcacccggaagccgtggcttcgaggcaatccgaaccggtggtgcagaaatgtcggagatagcttgacggatgtcccgtacggcaagtgaaaattcagggaataaggttgcggtgggtggcttcggtcttgccgcagcacgaaaatgaaggcgaagataagcatgtagaaagcaaattagttcctcgtttggacgagagttcgaatctctccagctccacatttcgggcgatca Predict ORF larger than 30AA ================================================ Protein_Len: 34 Strand: + Start: 215 End: 316 ...................................................................................................................................................................................................................... M K L N P Y T R C L D E T S P G S R G F E A I R T G G A E M S E I A .......................................................................................................................................................................... Protein_Len: 34 Strand: + Start: 238 End: 339 ............................................................................................................................................................................................................................................. M F G R D I T R K P W L R G N P N R W C R N V G D S L T D V P Y G K ................................................................................................................................................... Protein_Len: 49 Strand: + Start: 339 End: 485 .................................................................................................................................................................................................................................................................................................................................................. M K I Q G I R L R W V A S V L P Q H E N E G E D K H V E S K L V P R L D E S S N L S S S T F R A I . Protein_Len: 45 Strand: - Start: 350 End: 484 ............................................................................................................................................................................................................................................................................................................................................................. P I L N R H T A E T K G C C S F S P S S L C T S L L N T G R K S S L E F R E L E V N R A M .. Protein_Len: 30 Strand: - Start: 257 End: 346 ................................................................................................................................................................................................................................................................ G S A T A E L C D S G T T C F H R L Y S S P H G T R C T F M ............................................................................................................................................ Protein_Len: 60 Strand: - Start: 232 End: 474 ....................................................................................................................................................................................................................................... A S I D S I A Q R I D R V A L S F E P F L T A T P P K P R A A A R F H L R L Y A H L F C I L E E N P R S N S D R W S W M ............ Protein_Len: 60 Strand: - Start: 225 End: 416 ................................................................................................................................................................................................................................ T N P R S M V R F G H S R P L G F R H H L F T P S L K V S T G Y P L H F N L S Y P Q P P H S R D Q R L V F I F A F I L M ...................................................................... Protein_Len: 31 Strand: - Start: 70 End: 162 ..................................................................... L L P Q G T K V A P L L P K Y A H L A D N A P V K I E T S G M .................................................................................................................................................................................................................................................................................................................................... Protein_Len: 51 Strand: - Start: 63 End: 215 .............................................................. K K Y F P S V P K S L P C F H N T L M C H T T P R C K L R P Q D L K L Q R F Y S R E R Q K I S A C Y M ............................................................................................................................................................................................................................................................................... tttctaaacgatccgaagtgtcggatatggttaaatcatagaggtgattttcaaatcatttactttttatagaaggggctgaccggttttgacagcgggcagaagtggtttgtaagcatgcagtgcgtcgttggccggcactttaatctcggttgatccaattttaactggcgaaaataactacgctctcgctgcttaatcgaagcacagtagattgaagcttaatccttacacaaggtgtttggacgagacatcacccggaagccgtggcttcgaggcaatccgaaccggtggtgcagaaatgtcggagatagcttgacggatgtcccgtacggcaagtgaaaattcagggaataaggttgcggtgggtggcttcggtcttgccgcagcacgaaaatgaaggcgaagataagcatgtagaaagcaaattagttcctcgtttggacgagagttcgaatctctccagctccacatttcgggcgatca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tttctaaacgatccgaagtgtcggatatggttaaatcatagaggtgattttcaaatcatttactttttatagaaggggctgaccggttttgacagcgggcagaagtggtttgtaagcatgcagtgcgtcgttggccggcactttaatctcggttgatccaattttaactggcgaaaataactacgctctcgctgcttaatcgaagcacagtagattgaagcttaatccttacacaaggtgtttggacgagacatcacccggaagccgtggcttcgaggcaatccgaaccggtggtgcagaaatgtcggagatagcttgacggatgtcccgtacggcaagtgaaaattcagggaataaggttgcggtgggtggcttcggtcttgccgcagcacgaaaatgaaggcgaagataagcatgtagaaagcaaattagttcctcgtttggacgagagttcgaatctctccagctccacatttcgggcgatca Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF0527:TF0528|TF0527:TF0528:prolyl endopeptidase:two-component system sensor histidine kinase:->->:552011..552496 486 tttctaaacgatccgaagtgtcggatatggttaaatcatagaggtgattttcaaatcatttactttttatagaaggggctgaccggttttgacagcgggcagaagtggtttgtaagcatgcagtgcgtcgttggccggcactttaatctcggttgatccaattttaactggcgaaaataactacgctctcgctgcttaatcgaagcacagtagattgaagcttaatccttacacaaggtgtttggacgagacatcacccggaagccgtggcttcgaggcaatccgaaccggtggtgcagaaatgtcggagatagcttgacggatgtcccgtacggcaagtgaaaattcagggaataaggttgcggtgggtggcttcggtcttgccgcagcacgaaaatgaaggcgaagataagcatgtagaaagcaaattagttcctcgtttggacgagagttcgaatctctccagctccacatttcgggcgatca Intra-Species Hit: Count: 1 Min: 1 Max: 486 Len: 486 Subject: tfor_TF0527_TF0528|prolyl endopeptidase:two-component system sensor histidine kinase|POSITIVE:POSITIVE|[552011,552496]|486 HSP 1 e-value: 0.0 bit: 963.0 Len: 486 Query Start:1 Query End:486 Subject Strand: POSITIVE Subject Start: 1 Subject End: 486 tttctaaacgatccgaagtgtcggatatggttaaatcatagaggtgattttcaaatcatttactttttatagaaggggctgaccggttttgacagcgggcagaagtggtttgtaagcatgcagtgcgtcgttggccggcactttaatctcggttgatccaattttaactggcgaaaataactacgctctcgctgcttaatcgaagcacagtagattgaagcttaatccttacacaaggtgtttggacgagacatcacccggaagccgtggcttcgaggcaatccgaaccggtggtgcagaaatgtcggagatagcttgacggatgtcccgtacggcaagtgaaaattcagggaataaggttgcggtgggtggcttcggtcttgccgcagcacgaaaatgaaggcgaagataagcatgtagaaagcaaattagttcctcgtttggacgagagttcgaatctctccagctccacatttcgggcgatca tttctaaacgatccgaagtgtcggatatggttaaatcatagaggtgattttcaaatcatttactttttatagaaggggctgaccggttttgacagcgggcagaagtggtttgtaagcatgcagtgcgtcgttggccggcactttaatctcggttgatccaattttaactggcgaaaataactacgctctcgctgcttaatcgaagcacagtagattgaagcttaatccttacacaaggtgtttggacgagacatcacccggaagccgtggcttcgaggcaatccgaaccggtggtgcagaaatgtcggagatagcttgacggatgtcccgtacggcaagtgaaaattcagggaataaggttgcggtgggtggcttcggtcttgccgcagcacgaaaatgaaggcgaagataagcatgtagaaagcaaattagttcctcgtttggacgagagttcgaatctctccagctccacatttcgggcgatca Inter-species Hit: Count: 4 Min: 73 Max: 265 Len: 193 Subject: NC_006347_BF1127_BF1128|hypothetical protein:hypothetical protein|NEGATIVE:NEGATIVE|[1350671,1350862]|192 HSP 1 e-value: 1.0E-24 bit: 115.0 Len: 156 Query Start:73 Query End:228 Subject Strand: POSITIVE Subject Start: 14 Subject End: 167 ........................................................................aaggggctgaccggttttgacagcgggcagaagtggtttgtaagcatgcagtgcgtcgttggccggcactttaatctcggttgatc-caattttaactggcgaaaataactacgctctcgctgcttaatcgaagcacagtagattgaagcttaatcc.................................................................................................................................................................................................................................................................. ........................................................................aaggggttgactggttttgacagcgggcagaaatggtgggtaagcatgcagtgcgtcggtgatttgcactttaatctcagtt-atcaaaattttatctggcgaaactaattacgctcttgctgcttaatcgaatcatagtagatt--agcttaatcc.................................................................................................................................................................................................................................................................. Subject: NC_003228_BF1038_BF1040|hypothetical protein:hypothetical protein|NEGATIVE:NEGATIVE|[1289053,1289422]|370 HSP 1 e-value: 1.0E-24 bit: 115.0 Len: 156 Query Start:73 Query End:228 Subject Strand: POSITIVE Subject Start: 192 Subject End: 345 ........................................................................aaggggctgaccggttttgacagcgggcagaagtggtttgtaagcatgcagtgcgtcgttggccggcactttaatctcggttgatc-caattttaactggcgaaaataactacgctctcgctgcttaatcgaagcacagtagattgaagcttaatcc.................................................................................................................................................................................................................................................................. ........................................................................aaggggttgactggttttgacagcgggcagaaatggtgggtaagcatgcagtgcgtcggtgatttgcactttaatctcagtt-atcaaaattttatctggcgaaactaattacgctcttgctgcttaatcgaatcatagtagatt--agcttaatcc.................................................................................................................................................................................................................................................................. Subject: NC_002950_PG2117_PG2119|30S ribosomal protein S16:oxidoreductase, Gfo/Idh/MocA family|NEGATIVE:POSITIVE|[2225904,2226686]|783 HSP 1 e-value: 1.0E-15 bit: 85.7 Len: 142 Query Start:75 Query End:216 Subject Strand: NEGATIVE Subject Start: 418 Subject End: 559 ..........................................................................ggggctgaccggttttgacagcgggcagaagtggtttgtaagcatgcagtgcgtcgttggccggcactttaatctcggttgatccaat-tttaactggcgaaaataactacgctctcgctgcttaatcgaagcacagtagatt.............................................................................................................................................................................................................................................................................. ..........................................................................ggggctgaccggctttgacagcgtgatgaagcggtatgtaagcatgtagtgcgtgggtggcttgcactataatctcagac-atcaaaagtttaattggcgaaaataactacgctctcgctgcgtaatcgaagaatagtagatt.............................................................................................................................................................................................................................................................................. Subject: NC_004663_BT_4400_BT_4401|hypothetical protein:hypothetical protein|NEGATIVE:POSITIVE|[5797753,5798561]|809 HSP 1 e-value: 8.0E-14 bit: 79.8 Len: 103 Query Start:163 Query End:265 Subject Strand: POSITIVE Subject Start: 548 Subject End: 648 ..................................................................................................................................................................tttaactggcgaaaataactacgctctcgctgcttaatcgaagcacagtagattgaagcttaatccttacacaaggtgtttggacgagacatcacccggaagc............................................................................................................................................................................................................................. ..................................................................................................................................................................tttatctggcgaaactaattacgctcttgctgcttaatcgaatcacagtagatt--agcttaatccaggcactaggtgccaggacgagacatcactcggaagc............................................................................................................................................................................................................................. HSP 2 e-value: 1.0E-6 bit: 56.0 Len: 56 Query Start:75 Query End:130 Subject Strand: POSITIVE Subject Start: 460 Subject End: 515 ..........................................................................ggggctgaccggttttgacagcgggcagaagtggtttgtaagcatgcagtgcgtcg.................................................................................................................................................................................................................................................................................................................................................................... ..........................................................................ggggttgactggatttgacagcgggcagaaatggtaggtaagcatgcagtgggtcg.................................................................................................................................................................................................................................................................................................................................................................... Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAGGGGCUGACCGGUUUUGACAGCGGGCAGAAGUGGUUUGUAAGCAUGCAGUGCGUCGUUGGCCGGCACUUUAAUCUCGGUUGAUCCAAUUUUAACUGGCGAAAAUAACUACGCUCUCGCUGCUUAAUCGAAGCACAGUAGAUUGAAGCUUAAUCCUUACACAAGGUGUUUGGACGAGACAUCACCCGGAAGC ..(((.(((.((.(((.....))).)))))..(((.((((((((((.((((.((((.(((((((((....((((....((.....))....))))))))).....)))).)))).)).))))))))..)))).))).......(((.((((..(((..((((...))))..))).))).).))))))...... (-52.80) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GCUUCCGGGUGAUGUCUCGUCCAAACACCUUGUGUAAGGAUUAAGCUUCAAUCUACUGUGCUUCGAUUAAGCAGCGAGAGCGUAGUUAUUUUCGCCAGUUAAAAUUGGAUCAACCGAGAUUAAAGUGCCGGCCAACGACGCACUGCAUGCUUACAAACCACUUCUGCCCGCUGUCAAAACCGGUCAGCCCCUU (((.((((.((((.....((((..((((...))))..))))(((((...(((((....((.(((((((.(((.(((((((........)))))))..)))..))))))).))....)))))..(((((((.....))..)))))....)))))...................))))...))))..)))..... (-46.30) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 471 68 21 224 168 tfor:TF3135|5end_hypothetical_protein_3369821..3370051_POSITIVE 463 70 21 55 11 tfor:TF0665|5end_conserved_hypothetical_protein_709497..709727_POSITIVE 447 70 21 59 2 tfor:TF0953|5end_conserved_hypothetical_protein;_possible_DNA_binding_protein_997929..998159_POSITIVE 421 49 4 70 21 tfor:TF0777|5end_1,4-dihydroxy-2-naphthoate_octaprenyltransferase_826098..826328_POSITIVE 420 47 3 175 124 tfor:TF3029|5end_membrane_peptidase_3252803..3253033_POSITIVE 406 65 26 166 117 tfor:TF1475|5end_glucose-6-phosphate_1-dehydrogenase_1568034..1568264_POSITIVE 402 66 21 39 7 tfor:TF0013|5end_conserved_hypothetical_protein;_possible_TPR-repeat-containing_protein_6414..6644_POSITIVE 401 68 23 166 107 tfor:TF1260|5end_chaperone_protein_DnaJ_1345325..1345555_POSITIVE 398 67 21 163 99 tfor:TF0819|5end_phosphoglycerate_mutase_878447..878677_POSITIVE 395 70 23 210 154 tfor:TF0529|5end_transposase_554776..555006_POSITIVE 391 160 112 66 11 tfor:TF3154|5end_conserved_hypothetical_protein_3389219..3389449_POSITIVE 390 65 21 163 118 tfor:TF2992|5end_hexapeptide_transferase_family_protein_3209042..3209272_POSITIVE 388 49 5 190 138 tfor:TF0995|5end_conserved_hypothetical_protein_1049695..1049925_POSITIVE 384 78 37 205 159 tfor:TF1159|5end_hypothetical_protein_1240113..1240343_POSITIVE 382 67 23 210 152 tfor:TF2231|5end_DNA_methylase,_site-specific_DNA-methyltransferase_(adenine-specific)_2400307..2400537_POSITIVE 381 66 21 64 14 tfor:TF2809|5end_conserved_hypothetical_protein;_possible_translation_factor_(SUA5)_2999832..3000062_POSITIVE 378 67 21 82 25 tfor:TF2911|5end_hypothetical_protein_3105871..3106101_POSITIVE 377 67 21 60 8 tfor:TF0433|5end_60_kDa_inner_membrane_protein_453357..453587_POSITIVE 376 69 21 206 146 tfor:TF3039|5end_ABC_transporter,_ATP-binding_protein_3265731..3265961_POSITIVE 375 80 35 71 7 tfor:TF2499|5end_possible_thioesterase_family_protein_2673903..2674133_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 463 70 21 48 4 tfor:TF0664|3end_small_protein_B_(SMPB)_709570..709720_POSITIVE 447 70 21 59 2 tfor:TF0952|3end_conserved_hypothetical_protein_998009..998159_POSITIVE 440 68 21 68 7 tfor:TF2673|3end_conserved_hypothetical_protein_2848272..2848422_POSITIVE 425 67 24 142 105 tfor:TF0596|3end_conserved_hypothetical_protein_628652..628802_POSITIVE 421 49 4 65 16 tfor:TF0776|3end_ABC_transporter,_permease_component_826173..826323_POSITIVE 398 68 23 42 1 tfor:TF1261|3end_GrpE-related_chaperonin;_HSP-70_cofactor_1347192..1347342_POSITIVE 397 65 21 146 89 tfor:TF0818|3end_bifunctional_protein:__aspartokinase_I;_homoserine_dehydrogenase_878517..878667_POSITIVE 391 160 112 91 36 tfor:TF3153|3end_conserved_hypothetical_protein_3389324..3389474_POSITIVE 390 65 21 148 103 tfor:TF2991|3end_conserved_hypothetical_protein_3209107..3209257_POSITIVE 384 78 37 141 95 tfor:TF1158|3end_outer_membrane_lipoprotein,_NlpE_involved_in_copper_resistance_1240129..1240279_POSITIVE 382 68 22 151 104 tfor:TF2600|3end_ribose-phosphate_pyrophosphokinase_2774709..2774859_POSITIVE 381 66 21 61 11 tfor:TF2808|3end_bacterial_sugar_transferase_2999909..3000059_POSITIVE 378 67 21 87 30 tfor:TF2909|3end_GTP-binding_protein,_possible_GTP1/OBG_family_3105956..3106106_POSITIVE 375 66 23 94 42 tfor:TF0145|3end_glutamine_synthetase_154718..154868_POSITIVE 371 68 24 76 36 tfor:TF0247|3end_conserved_hypothetical_protein_278348..278498_POSITIVE 366 67 24 48 5 tfor:TF0960|3end_beta-galactosidase_1010506..1010656_POSITIVE 363 70 21 142 91 tfor:TF3033|3end_S1_RNA_binding_domain_protein,_S1_ribosomal_protein_3258983..3259133_POSITIVE 357 66 23 115 71 tfor:TF2335|3end_conserved_hypothetical_protein_2496177..2496327_POSITIVE 356 68 22 145 82 tfor:TF0386|3end_transcriptional_regulator,_possible_LuxR_family_407579..407729_POSITIVE 355 70 25 151 93 tfor:TF2774|3end_2-amino-4-hydroxy-6-hydroxymethyldihydropteridine_pyrophosphokinase_2959719..2959869_POSITIVE