Origin IGS: acgttacggataaaccgccggcaaagatacgatttttgtactgctttctcgggtttcggcaggcgaacgcgctctttgcacaagaaaaagaaaagccgcaagcaaaatcgttcttgatccgttttgcctgcggcccttatagaccatgaatcgattttgttggttta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taaaccaacaaaatcgattcatggtctataagggccgcaggcaaaacggatcaagaacgattttgcttgcggcttttctttttcttgtgcaaagagcgcgttcgcctgccgaaacccgagaaagcagtacaaaaatcgtatctttgccggcggtttatccgtaacgt Mask Tandem Repeat Region ================================================ acgttacggataaaccgccggcaaagatacgatttttgtactgctttctcgggtttcggcaggcgaacgcgctctttgcacaagaaaaagaaaagccgcaagcaaaatcgttcttgatccgttttgcctgcggcccttatagaccatgaatcgattttgttggttta Find is-nt database================================================ Query_seq: TF0525:TF0527|TF0525:TF0527:conserved hypothetical protein:prolyl endopeptidase:->->:549645..549811 167 acgttacggataaaccgccggcaaagatacgatttttgtactgctttctcgggtttcggcaggcgaacgcgctctttgcacaagaaaaagaaaagccgcaagcaaaatcgttcttgatccgttttgcctgcggcccttatagaccatgaatcgattttgttggttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF0525:TF0527|TF0525:TF0527:conserved hypothetical protein:prolyl endopeptidase:->->:549645..549811 167 acgttacggataaaccgccggcaaagatacgatttttgtactgctttctcgggtttcggcaggcgaacgcgctctttgcacaagaaaaagaaaagccgcaagcaaaatcgttcttgatccgttttgcctgcggcccttatagaccatgaatcgattttgttggttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF0525:TF0527|TF0525:TF0527:conserved hypothetical protein:prolyl endopeptidase:->->:549645..549811 167 acgttacggataaaccgccggcaaagatacgatttttgtactgctttctcgggtttcggcaggcgaacgcgctctttgcacaagaaaaagaaaagccgcaagcaaaatcgttcttgatccgttttgcctgcggcccttatagaccatgaatcgattttgttggttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 acgttacggataaaccgccggcaaagatacgatttttgtactgctttctcgggtttcggcaggcgaacgcgctctttgcacaagaaaaagaaaagccgcaagcaaaatcgttcttgatccgttttgcctgcggcccttatagaccatgaatcgattttgttggttta Predict ORF larger than 30AA ================================================ Protein_Len: 35 Strand: + Start: 10 End: 114 ......... M N R R Q R Y D F C T A F S G F G R R T R S L H K K K K S R K Q N R S ..................................................... Protein_Len: 36 Strand: + Start: 32 End: 139 ............................... M F V L L S R V S A G E R A L C T R K R K A A S K I V L D P F C L R P L ............................ Protein_Len: 40 Strand: + Start: 27 End: 146 .......................... M R F L Y C F L G F R Q A N A L F A Q E K E K P Q A K S F L I R F A C G P Y R P ..................... Protein_Len: 47 Strand: - Start: 7 End: 147 ...... P Y V A P L S V I K T S S E R T E A P S R A R Q V L F L F A A L L I T R S G N Q R R G K Y V M .................... Protein_Len: 51 Strand: - Start: 3 End: 155 .. N R I F R R C L Y S K Q V A K E P K P L R V R E K C L F F F L R L C F R E Q D T K G A A R I S W S D M ............ Protein_Len: 39 Strand: - Start: 2 End: 118 . T V S L G G A F I R N K Y Q K R P N R C A F A S K A C S F S F G C A F D N K M ................................................. acgttacggataaaccgccggcaaagatacgatttttgtactgctttctcgggtttcggcaggcgaacgcgctctttgcacaagaaaaagaaaagccgcaagcaaaatcgttcttgatccgttttgcctgcggcccttatagaccatgaatcgattttgttggttta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ acgttacggataaaccgccggcaaagatacgatttttgtactgctttctcgggtttcggcaggcgaacgcgctctttgcacaagaaaaagaaaagccgcaagcaaaatcgttcttgatccgttttgcctgcggcccttatagaccatgaatcgattttgttggttta Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 100 HP_score: -10.9 Tail_Score: -5.44638 Start: 95 End: 134 Full_Region: tcatggtctataagg gccgcaggcaaaacg gatcaagaa cgattttgcttgcggc ttttctttttcttgt ..............................................................................................gccgcaggcaaaacggatcaagaacgattttgcttgcggc................................. Find igs database================================================ Query_seq: TF0525:TF0527|TF0525:TF0527:conserved hypothetical protein:prolyl endopeptidase:->->:549645..549811 167 acgttacggataaaccgccggcaaagatacgatttttgtactgctttctcgggtttcggcaggcgaacgcgctctttgcacaagaaaaagaaaagccgcaagcaaaatcgttcttgatccgttttgcctgcggcccttatagaccatgaatcgattttgttggttta Intra-Species Hit: Count: 1 Min: 1 Max: 167 Len: 167 Subject: tfor_TF0525_TF0527|conserved hypothetical protein:prolyl endopeptidase|POSITIVE:POSITIVE|[549645,549811]|167 HSP 1 e-value: 4.0E-90 bit: 331.0 Len: 167 Query Start:1 Query End:167 Subject Strand: POSITIVE Subject Start: 1 Subject End: 167 acgttacggataaaccgccggcaaagatacgatttttgtactgctttctcgggtttcggcaggcgaacgcgctctttgcacaagaaaaagaaaagccgcaagcaaaatcgttcttgatccgttttgcctgcggcccttatagaccatgaatcgattttgttggttta acgttacggataaaccgccggcaaagatacgatttttgtactgctttctcgggtttcggcaggcgaacgcgctctttgcacaagaaaaagaaaagccgcaagcaaaatcgttcttgatccgttttgcctgcggcccttatagaccatgaatcgattttgttggttta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ ACGUUACGGAUAAACCGCCGGCAAAGAUACGAUUUUUGUACUGCUUUCUCGGGUUUCGGCAGGCGAACGCGCUCUUUGCACAAGAAAAAGAAAAGCCGCAAGCAAAAUCGUUCUUGAUCCGUUUUGCCUGCGGCCCUUAUAGACCAUGAAUCGAUUUUGUUGGUUUA ................((((((((((...((((((.((..(((.(((((....((((.(((((((....))))...)))....)))).))))).((((((.(((((((.(........).))))))).)))))).....)))..)).)))))).))))))))))... (-47.40) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAACCAACAAAAUCGAUUCAUGGUCUAUAAGGGCCGCAGGCAAAACGGAUCAAGAACGAUUUUGCUUGCGGCUUUUCUUUUUCUUGUGCAAAGAGCGCGUUCGCCUGCCGAAACCCGAGAAAGCAGUACAAAAAUCGUAUCUUUGCCGGCGGUUUAUCCGUAACGU ........((((..(((((..((..((..(((((((((((((((((((.........)).)))))))))))))))))(((((((..((((.....)))).((((.....))))....))))))).))..))..)))))....))))...((((.....))))..... (-48.10) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 431 100 53 212 165 tfor:TF0597|5end_hypothetical_protein_628788..629018_POSITIVE 421 80 42 45 1 tfor:TF0553|5end_Bacteroides_aerotolerance_protein,_BatD_584462..584692_POSITIVE 386 152 111 83 45 tfor:TF0170|5end_possible_CRISPR-associated_protein_Cas2_179566..179796_POSITIVE 375 60 15 161 113 tfor:TF0307|5end_conserved_hypothetical_protein_332783..333013_POSITIVE 371 69 37 125 90 tfor:TF1763|5end_K+-dependent_Na+/Ca+_exchanger_related-protein_1900406..1900636_POSITIVE 370 79 41 231 184 tfor:TF1256|5end_hypothetical_protein_1338083..1338313_POSITIVE 369 73 35 58 5 tfor:TF1023|5end_ABC_transporter,_permease_component_1079197..1079427_POSITIVE 367 80 31 213 145 tfor:TF2214|5end_peptidyl-prolyl_cis-trans_isomerase_2386897..2387127_POSITIVE 364 70 21 174 121 tfor:TF0615|5end_mannose-1-phosphate_guanylyltransferase_648832..649062_POSITIVE 363 136 96 174 139 tfor:TF2259|5end_transfer_region-related_protein,_TraK_2429577..2429807_POSITIVE 359 58 16 230 186 tfor:TF1049|5end_cobyrinic_acid_a,c-diamide_synthase_1110140..1110370_POSITIVE 358 81 41 191 136 tfor:TF2430|5end_hypothetical_protein_2612660..2612890_POSITIVE 353 60 19 61 19 tfor:TF2738|5end_30S_ribosomal_protein_S16_2919303..2919533_POSITIVE 353 67 23 64 18 tfor:TF2498|5end_electron_transfer_flavoprotein,_alpha_subunit_2672801..2673031_POSITIVE 352 135 95 230 194 tfor:TF1598|5end_conserved_hypothetical_protein_1713358..1713588_POSITIVE 350 74 37 137 92 tfor:TF2912|5end_hypothetical_protein_3106508..3106738_POSITIVE 350 78 31 189 128 tfor:TF2255|5end_transfer_region-related_protein,_TraH_2427504..2427734_POSITIVE 349 53 15 42 9 tfor:TF2998|5end_surface_antigen_BspA_3211811..3212041_POSITIVE 349 60 16 116 65 tfor:TF0065|5end_conserved_hypothetical_protein_70274..70504_POSITIVE 347 79 38 72 20 tfor:TF0301|5end_outer_membrane_protein,_TonB_dependent_receptor_325457..325687_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 403 60 17 70 28 tfor:TF0178|3end_ferritin_188223..188373_POSITIVE 387 133 94 126 82 tfor:TF1738|3end_outer_membrane_protein_1866946..1867096_POSITIVE 386 152 111 80 42 tfor:TF0169|3end_possible_CRISPR-associated_protein_Cas1_179643..179793_POSITIVE 364 70 21 125 72 tfor:TF0614|3end_UDP-glucose_6-dehydrogenase/UDP-N-acetyl-D-mannosaminuronate_dehydrogenase_648863..649013_POSITIVE 363 136 96 139 104 tfor:TF2257|3end_transfer_region-related_protein,_TraJ_2429622..2429772_POSITIVE 360 99 51 68 14 tfor:TF2260|3end_transfer_region-related_protein,_TraL_2430592..2430742_POSITIVE 360 83 43 147 91 tfor:TF0624|3end_Xaa-Pro_dipeptidase_(aminopeptidase_P)_661015..661165_POSITIVE 350 78 31 136 75 tfor:TF2253|3end_conjugative_transposon_protein,_TraG_2427531..2427681_POSITIVE 347 65 21 48 6 tfor:TF0306|3end_transglutaminase-like_enzyme/cysteine_protease_332749..332899_POSITIVE 347 79 38 76 24 tfor:TF0300|3end_hypothetical_protein_325541..325691_POSITIVE 344 102 62 44 8 tfor:TF2243|3end_possible_mobilization_protein_2419470..2419620_POSITIVE 338 136 93 76 28 tfor:TF1296|3end_possible_DNA-binding_protein,_histone-like_family_1378982..1379132_POSITIVE 338 98 54 108 62 tfor:TF0309|3end_conserved_hypothetical_protein_334775..334925_POSITIVE 337 77 31 151 86 tfor:TF2581|3end_50S_ribosomal_protein_L17_2749370..2749520_POSITIVE 336 76 37 59 5 tfor:TF2455|3end_conserved_hypothetical_protein;_possible_membrane_protein_2632437..2632587_POSITIVE 334 153 115 50 4 tfor:TF2807|3end_possible_chloride_channel_protein_2998534..2998684_POSITIVE 333 138 95 138 94 tfor:TF2042|3end_sugar_phosphate_isomerase,_polysialic_acid_capsule_expression_protein_2202356..2202506_POSITIVE 330 59 17 71 16 tfor:TF0964|3end_conserved_hypothetical_protein_1013376..1013526_POSITIVE 330 143 107 67 31 tfor:TF0175|3end_hypothetical_protein_185907..186057_POSITIVE 327 77 42 147 105 tfor:TF2426|3end_conserved_hypothetical_protein;_possible_TonB_receptor_2605931..2606081_POSITIVE