Origin IGS: tgctgttgatttttgatatcttttacgctgcaaagataaagattagcgcctaatgtacaatggtctttggaatgaagttatcaacagagaaacatgtttattgcagatgaaaaaccttttatctgctttcatgaagtcaaacaatcatggagtgttgaggttccggtgtcggaaacaagttgcaggaagtcgtctttaaggcatgcaaaaaaaaaaatca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tgatttttttttttgcatgccttaaagacgacttcctgcaacttgtttccgacaccggaacctcaacactccatgattgtttgacttcatgaaagcagataaaaggtttttcatctgcaataaacatgtttctctgttgataacttcattccaaagaccattgtacattaggcgctaatctttatctttgcagcgtaaaagatatcaaaaatcaacagca Mask Tandem Repeat Region ================================================ tgctgttgatttttgatatcttttacgctgcaaagataaagattagcgcctaatgtacaatggtctttggaatgaagttatcaacagagaaacatgtttattgcagatgaaaaaccttttatctgctttcatgaagtcaaacaatcatggagtgttgaggttccggtgtcggaaacaagttgcaggaagtcgtctttaaggcatgcaaaaaaaaaaatca Find is-nt database================================================ Query_seq: TF0518:TF0519|TF0518:TF0519:glutaminyl-tRNA synthetase:thiamine monophosphate kinase:->->:543620..543839 220 tgctgttgatttttgatatcttttacgctgcaaagataaagattagcgcctaatgtacaatggtctttggaatgaagttatcaacagagaaacatgtttattgcagatgaaaaaccttttatctgctttcatgaagtcaaacaatcatggagtgttgaggttccggtgtcggaaacaagttgcaggaagtcgtctttaaggcatgcaaaaaaaaaaatca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF0518:TF0519|TF0518:TF0519:glutaminyl-tRNA synthetase:thiamine monophosphate kinase:->->:543620..543839 220 tgctgttgatttttgatatcttttacgctgcaaagataaagattagcgcctaatgtacaatggtctttggaatgaagttatcaacagagaaacatgtttattgcagatgaaaaaccttttatctgctttcatgaagtcaaacaatcatggagtgttgaggttccggtgtcggaaacaagttgcaggaagtcgtctttaaggcatgcaaaaaaaaaaatca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF0518:TF0519|TF0518:TF0519:glutaminyl-tRNA synthetase:thiamine monophosphate kinase:->->:543620..543839 220 tgctgttgatttttgatatcttttacgctgcaaagataaagattagcgcctaatgtacaatggtctttggaatgaagttatcaacagagaaacatgtttattgcagatgaaaaaccttttatctgctttcatgaagtcaaacaatcatggagtgttgaggttccggtgtcggaaacaagttgcaggaagtcgtctttaaggcatgcaaaaaaaaaaatca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tgctgttgatttttgatatcttttacgctgcaaagataaagattagcgcctaatgtacaatggtctttggaatgaagttatcaacagagaaacatgtttattgcagatgaaaaaccttttatctgctttcatgaagtcaaacaatcatggagtgttgaggttccggtgtcggaaacaagttgcaggaagtcgtctttaaggcatgcaaaaaaaaaaatca Predict ORF larger than 30AA ================================================ Protein_Len: 48 Strand: + Start: 53 End: 196 .................................................... M Y N G L W N E V I N R E T C L L Q M K N L L S A F M K S N N H G V L R F R C R K Q V A G S R L ........................ Protein_Len: 42 Strand: + Start: 94 End: 219 ............................................................................................. M F I A D E K P F I C F H E V K Q S W S V E V P V S E T S C R K S S L R H A K K K I . Protein_Len: 33 Strand: - Start: 46 End: 144 ............................................. R R I Y L P R Q F S T I L L S V H K N C I F F R K D A K M F D F M ............................................................................ tgctgttgatttttgatatcttttacgctgcaaagataaagattagcgcctaatgtacaatggtctttggaatgaagttatcaacagagaaacatgtttattgcagatgaaaaaccttttatctgctttcatgaagtcaaacaatcatggagtgttgaggttccggtgtcggaaacaagttgcaggaagtcgtctttaaggcatgcaaaaaaaaaaatca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tgctgttgatttttgatatcttttacgctgcaaagataaagattagcgcctaatgtacaatggtctttggaatgaagttatcaacagagaaacatgtttattgcagatgaaaaaccttttatctgctttcatgaagtcaaacaatcatggagtgttgaggttccggtgtcggaaacaagttgcaggaagtcgtctttaaggcatgcaaaaaaaaaaatca Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 76 HP_score: -9.0 Tail_Score: -3.74693 Start: 103 End: 126 Full_Region: AGAAACATGTTTATT GCAGATGAAA AACC TTTTATCTGC TTTCATGAAGTCAAA ......................................................................................................GCAGATGAAAAACCTTTTATCTGC.............................................................................................. Find igs database================================================ Query_seq: TF0518:TF0519|TF0518:TF0519:glutaminyl-tRNA synthetase:thiamine monophosphate kinase:->->:543620..543839 220 tgctgttgatttttgatatcttttacgctgcaaagataaagattagcgcctaatgtacaatggtctttggaatgaagttatcaacagagaaacatgtttattgcagatgaaaaaccttttatctgctttcatgaagtcaaacaatcatggagtgttgaggttccggtgtcggaaacaagttgcaggaagtcgtctttaaggcatgcaaaaaaaaaaatca Intra-Species Hit: Count: 1 Min: 1 Max: 206 Len: 206 Subject: tfor_TF0518_TF0519|glutaminyl-tRNA synthetase:thiamine monophosphate kinase|POSITIVE:POSITIVE|[543620,543839]|220 HSP 1 e-value: 1.0E-113 bit: 408.0 Len: 206 Query Start:1 Query End:206 Subject Strand: POSITIVE Subject Start: 1 Subject End: 206 tgctgttgatttttgatatcttttacgctgcaaagataaagattagcgcctaatgtacaatggtctttggaatgaagttatcaacagagaaacatgtttattgcagatgaaaaaccttttatctgctttcatgaagtcaaacaatcatggagtgttgaggttccggtgtcggaaacaagttgcaggaagtcgtctttaaggcatgc.............. tgctgttgatttttgatatcttttacgctgcaaagataaagattagcgcctaatgtacaatggtctttggaatgaagttatcaacagagaaacatgtttattgcagatgaaaaaccttttatctgctttcatgaagtcaaacaatcatggagtgttgaggttccggtgtcggaaacaagttgcaggaagtcgtctttaaggcatgc.............. Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGCUGUUGAUUUUUGAUAUCUUUUACGCUGCAAAGAUAAAGAUUAGCGCCUAAUGUACAAUGGUCUUUGGAAUGAAGUUAUCAACAGAGAAACAUGUUUAUUGCAGAUGAAAAACCUUUUAUCUGCUUUCAUGAAGUCAAACAAUCAUGGAGUGUUGAGGUUCCGGUGUCGGAAACAAGUUGCAGGAAGUCGUCUUUAAGGCAUGC .((.(((((((((...(((((((........)))))))))))))))))).............(((((.(((.(((..((..((((.....(((((.......((((((((((....)))))))))).(((((((..........))))))))))))..(.(((((....))))).)..))))...))..))))))..))))).... (-47.80) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GCAUGCCUUAAAGACGACUUCCUGCAACUUGUUUCCGACACCGGAACCUCAACACUCCAUGAUUGUUUGACUUCAUGAAAGCAGAUAAAAGGUUUUUCAUCUGCAAUAAACAUGUUUCUCUGUUGAUAACUUCAUUCCAAAGACCAUUGUACAUUAGGCGCUAAUCUUUAUCUUUGCAGCGUAAAAGAUAUCAAAAAUCAACAGCA ((..((((.....((((...............(((((....)))))..((((((...(((((..........)))))...((((((.(((....))).))))))................))))))....................)))).....)))).........(((((((........))))))).............)). (-37.70) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 411 186 147 158 113 tfor:TF0851|5end_helicase_SNF2_family_899486..899716_POSITIVE 355 187 143 230 186 tfor:TF2694|5end_hypothetical_protein_2870403..2870633_POSITIVE 343 187 147 82 45 tfor:TF2038|5end_hypothetical_protein_2198978..2199208_POSITIVE 331 187 152 71 36 tfor:TF0230|5end_hydrolase,_haloacid_dehalogenase-like_family_256982..257212_POSITIVE 329 187 148 78 33 tfor:TF0782|5end_ThiJ/PfpI_family_protein_835976..836206_POSITIVE 321 187 148 123 79 tfor:TF0625|5end_hypothetical_protein_661143..661373_POSITIVE 319 70 26 49 6 tfor:TF1919|5end_hypothetical_protein_2070037..2070267_POSITIVE 315 190 147 198 161 tfor:TF1934|5end_hypothetical_protein_2083787..2084017_POSITIVE 314 187 141 209 148 tfor:TF2207|5end_exo-alpha-sialidase_(neuraminidase)_2380907..2381137_POSITIVE 311 180 131 53 6 tfor:TF1304|5end_conserved_hypothetical_protein_1385008..1385238_POSITIVE 310 196 152 210 147 tfor:TF1587|5end_conserved_hypothetical_protein_1700245..1700475_POSITIVE 310 197 151 52 10 tfor:TF1142|5end_outer_membrane_cobalamin_receptor_protein_1219207..1219437_POSITIVE 308 66 26 154 112 tfor:TF2789|5end_enoyl-[acyl-carrier-protein]_reductase_2972431..2972661_POSITIVE 307 194 152 192 147 tfor:TF1910|5end_nicotinate-nucleotide--dimethylbenzimidazole_phosphoribosyltransferase_2064001..2064231_POSITIVE 306 187 144 60 6 tfor:TF2377|5end_orotidine_5'_phosphate_decarboxylase_2541799..2542029_POSITIVE 306 172 147 179 155 tfor:TF1468|5end_beta-galactosidase_1557785..1558015_POSITIVE 306 195 151 74 20 tfor:TF0225|5end_iron-containing_alcohol_dehydrogenase_251477..251707_POSITIVE 304 192 152 205 169 tfor:TF3109|5end_hypothetical_protein_3341998..3342228_POSITIVE 303 179 148 119 87 tfor:TF2594|5end_hypothetical_protein_2764176..2764406_POSITIVE 302 68 22 180 126 tfor:TF0368|5end_hypothetical_protein_385401..385631_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 306 172 147 151 127 tfor:TF1467|3end_conserved_hypothetical_protein_1557837..1557987_POSITIVE 306 195 151 64 10 tfor:TF0224|3end_N-acetylglucosamine-6-phosphate_deacetylase_251547..251697_POSITIVE 303 179 148 105 73 tfor:TF2593|3end_amino_acid_transport_protein_2764242..2764392_POSITIVE 298 195 159 83 46 tfor:TF0233|3end_DNA_polymerase_III_gamma/tau_subunit_260556..260706_POSITIVE 294 196 151 71 25 tfor:TF2260|3end_transfer_region-related_protein,_TraL_2430592..2430742_POSITIVE 294 198 156 139 101 tfor:TF0217|3end_50S_ribosomal_protein_L35_243194..243344_POSITIVE 293 196 151 149 100 tfor:TF2020|3end_muconate_cycloisomerase_2177552..2177702_POSITIVE 292 186 141 58 6 tfor:TF1422|3end_GLCNAC_transferase_(UDP-N-acetylglucosamine-N-acetylmuramyl-(pentapeptide)-pyr_ophosphoryl-undecaprenol_N-acetylglucosamine_transferase)_1505823..1505973_POSITIVE 290 186 144 140 97 tfor:TF2149|3end_deoxyribose-phosphate_aldolase_2323110..2323260_POSITIVE 290 187 146 79 30 tfor:TF1823|3end_xanthan_lyase_XalB_precursor_1972589..1972739_POSITIVE 288 117 72 54 6 tfor:TF0059|3end_hypothetical_protein_61720..61870_POSITIVE 285 186 147 82 47 tfor:TF1247|3end_alanyl-tRNA_synthetase_(alanine--tRNA_ligase)_1330928..1331078_POSITIVE 285 199 157 56 15 tfor:TF0556|3end_Bacteroides_aerotolerance_protein,_BatA_589149..589299_POSITIVE 285 180 134 66 14 tfor:TF0348|3end_hypothetical_protein_370447..370597_POSITIVE 284 190 145 39 1 tfor:TF3065|3end_enolase_3292412..3292562_POSITIVE 284 190 143 113 60 tfor:TF1764|3end_transcriptional_regulator;_possible_MerR_family_1901848..1901998_POSITIVE 283 194 151 58 10 tfor:TF2321|3end_hypothetical_protein_2482657..2482807_POSITIVE 283 198 152 106 56 tfor:TF0982|3end_conserved_hypothetical_protein_1039474..1039624_POSITIVE 282 194 151 79 32 tfor:TF1795|3end_conserved_hypothetical_protein_1936476..1936626_POSITIVE 282 200 151 123 68 tfor:TF1599|3end_ribonuclease_P_protein_component_1714054..1714204_POSITIVE