Origin IGS: tccatttcgtatgttaaatgaactttttgtgagtgcaaaggacggtcgttatgtggttatggacaatccctaataattggtattttttgtcttgttatccctatttttggctgtttgattttctctttttgtctgaacttatcggctcttttgtgaatttttatcgtgtgtaatcggacgtttagttgttgagatgtttgtttagtattcagccgtcagcgttcagccaactcaacaattcaacatctcaacaactaaacaataaacaactcaacatttatttcctcaggcttacagccttccgacgggatgtgatgccttttccccaacgcttcgcattgggctttgaatctacttacctcagaacaagtacatgtatca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tgatacatgtacttgttctgaggtaagtagattcaaagcccaatgcgaagcgttggggaaaaggcatcacatcccgtcggaaggctgtaagcctgaggaaataaatgttgagttgtttattgtttagttgttgagatgttgaattgttgagttggctgaacgctgacggctgaatactaaacaaacatctcaacaactaaacgtccgattacacacgataaaaattcacaaaagagccgataagttcagacaaaaagagaaaatcaaacagccaaaaatagggataacaagacaaaaaataccaattattagggattgtccataaccacataacgaccgtcctttgcactcacaaaaagttcatttaacatacgaaatgga Mask Tandem Repeat Region ================================================ tccatttcgtatgttaaatgaactttttgtgagtgcaaaggacggtcgttatgtggttatggacaatccctaataattggtattttttgtcttgttatccctatttttggctgtttgattttctctttttgtctgaacttatcggctcttttgtgaatttttatcgtgtgtaatcggacgtttagttgttgagatgtttgtttagtattcagccgtcagcgttcagcnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntttatttcctcaggcttacagccttccgacgggatgtgatgccttttccccaacgcttcgcattgggctttgaatctacttacctcagaacaagtacatgtatca Find is-nt database================================================ Query_seq: TF0498:TF0499|TF0498:TF0499:hypothetical protein:hypothetical protein:->->:526016..526396 381 tccatttcgtatgttaaatgaactttttgtgagtgcaaaggacggtcgttatgtggttatggacaatccctaataattggtattttttgtcttgttatccctatttttggctgtttgattttctctttttgtctgaacttatcggctcttttgtgaatttttatcgtgtgtaatcggacgtttagttgttgagatgtttgtttagtattcagccgtcagcgttcagccaactcaacaattcaacatctcaacaactaaacaataaacaactcaacatttatttcctcaggcttacagccttccgacgggatgtgatgccttttccccaacgcttcgcattgggctttgaatctacttacctcagaacaagtacatgtatca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF0498:TF0499|TF0498:TF0499:hypothetical protein:hypothetical protein:->->:526016..526396 381 tccatttcgtatgttaaatgaactttttgtgagtgcaaaggacggtcgttatgtggttatggacaatccctaataattggtattttttgtcttgttatccctatttttggctgtttgattttctctttttgtctgaacttatcggctcttttgtgaatttttatcgtgtgtaatcggacgtttagttgttgagatgtttgtttagtattcagccgtcagcgttcagccaactcaacaattcaacatctcaacaactaaacaataaacaactcaacatttatttcctcaggcttacagccttccgacgggatgtgatgccttttccccaacgcttcgcattgggctttgaatctacttacctcagaacaagtacatgtatca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF0498:TF0499|TF0498:TF0499:hypothetical protein:hypothetical protein:->->:526016..526396 381 tccatttcgtatgttaaatgaactttttgtgagtgcaaaggacggtcgttatgtggttatggacaatccctaataattggtattttttgtcttgttatccctatttttggctgtttgattttctctttttgtctgaacttatcggctcttttgtgaatttttatcgtgtgtaatcggacgtttagttgttgagatgtttgtttagtattcagccgtcagcgttcagccaactcaacaattcaacatctcaacaactaaacaataaacaactcaacatttatttcctcaggcttacagccttccgacgggatgtgatgccttttccccaacgcttcgcattgggctttgaatctacttacctcagaacaagtacatgtatca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tccatttcgtatgttaaatgaactttttgtgagtgcaaaggacggtcgttatgtggttatggacaatccctaataattggtattttttgtcttgttatccctatttttggctgtttgattttctctttttgtctgaacttatcggctcttttgtgaatttttatcgtgtgtaatcggacgtttagttgttgagatgtttgtttagtattcagccgtcagcgttcagccaactcaacaattcaacatctcaacaactaaacaataaacaactcaacatttatttcctcaggcttacagccttccgacgggatgtgatgccttttccccaacgcttcgcattgggctttgaatctacttacctcagaacaagtacatgtatca Predict ORF larger than 30AA ================================================ Protein_Len: 46 Strand: + Start: 33 End: 170 ................................ M Q R T V V M W L W T I P N N W Y F L S C Y P Y F W L F D F L F L S E L I G S F V N F Y R V ................................................................................................................................................................................................................... Protein_Len: 60 Strand: + Start: 186 End: 380 ......................................................................................................................................................................................... M L R C L F S I Q P S A F S Q L N N S T S Q Q L N N K Q L N I Y F L R L T A F R R D V M P F P Q R F A L G F E S T Y L R . Protein_Len: 51 Strand: - Start: 60 End: 212 ........................................................... P C D R I I P I K Q R T I G I K P Q K I K E K Q R F K D A R K H I K I T H L R V N L Q Q S T Q K T N M ......................................................................................................................................................................... tccatttcgtatgttaaatgaactttttgtgagtgcaaaggacggtcgttatgtggttatggacaatccctaataattggtattttttgtcttgttatccctatttttggctgtttgattttctctttttgtctgaacttatcggctcttttgtgaatttttatcgtgtgtaatcggacgtttagttgttgagatgtttgtttagtattcagccgtcagcgttcagccaactcaacaattcaacatctcaacaactaaacaataaacaactcaacatttatttcctcaggcttacagccttccgacgggatgtgatgccttttccccaacgcttcgcattgggctttgaatctacttacctcagaacaagtacatgtatca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tccatttcgtatgttaaatgaactttttgtgagtgcaaaggacggtcgttatgtggttatggacaatccctaataattggtattttttgtcttgttatccctatttttggctgtttgattttctctttttgtctgaacttatcggctcttttgtgaatttttatcgtgtgtaatcggacgtttagttgttgagatgtttgtttagtattcagccgtcagcgttcagccaactcaacaattcaacatctcaacaactaaacaataaacaactcaacatttatttcctcaggcttacagccttccgacgggatgtgatgccttttccccaacgcttcgcattgggctttgaatctacttacctcagaacaagtacatgtatca Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF0498:TF0499|TF0498:TF0499:hypothetical protein:hypothetical protein:->->:526016..526396 381 tccatttcgtatgttaaatgaactttttgtgagtgcaaaggacggtcgttatgtggttatggacaatccctaataattggtattttttgtcttgttatccctatttttggctgtttgattttctctttttgtctgaacttatcggctcttttgtgaatttttatcgtgtgtaatcggacgtttagttgttgagatgtttgtttagtattcagccgtcagcgttcagccaactcaacaattcaacatctcaacaactaaacaataaacaactcaacatttatttcctcaggcttacagccttccgacgggatgtgatgccttttccccaacgcttcgcattgggctttgaatctacttacctcagaacaagtacatgtatca Intra-Species Hit: Count: 1 Min: 1 Max: 381 Len: 381 Subject: tfor_TF0498_TF0499|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[526016,526396]|381 HSP 1 e-value: 1.0E-52 bit: 208.0 Len: 105 Query Start:277 Query End:381 Subject Strand: POSITIVE Subject Start: 277 Subject End: 381 ....................................................................................................................................................................................................................................................................................tttatttcctcaggcttacagccttccgacgggatgtgatgccttttccccaacgcttcgcattgggctttgaatctacttacctcagaacaagtacatgtatca ....................................................................................................................................................................................................................................................................................tttatttcctcaggcttacagccttccgacgggatgtgatgccttttccccaacgcttcgcattgggctttgaatctacttacctcagaacaagtacatgtatca HSP 2 e-value: 6.0E-39 bit: 163.0 Len: 82 Query Start:1 Query End:82 Subject Strand: POSITIVE Subject Start: 1 Subject End: 82 tccatttcgtatgttaaatgaactttttgtgagtgcaaaggacggtcgttatgtggttatggacaatccctaataattggta........................................................................................................................................................................................................................................................................................................... tccatttcgtatgttaaatgaactttttgtgagtgcaaaggacggtcgttatgtggttatggacaatccctaataattggta........................................................................................................................................................................................................................................................................................................... HSP 3 e-value: 8.0E-29 bit: 129.0 Len: 65 Query Start:163 Query End:227 Subject Strand: POSITIVE Subject Start: 163 Subject End: 227 ..................................................................................................................................................................atcgtgtgtaatcggacgtttagttgttgagatgtttgtttagtattcagccgtcagcgttcagc.......................................................................................................................................................... ..................................................................................................................................................................atcgtgtgtaatcggacgtttagttgttgagatgtttgtttagtattcagccgtcagcgttcagc.......................................................................................................................................................... Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UCCAUUUCGUAUGUUAAAUGAACUUUUUGUGAGUGCAAAGGACGGUCGUUAUGUGGUUAUGGACAAUCCCUAAUAAUUGGUAUUUUUUGUCUUGUUAUCCCUAUUUUUGGCUGUUUGAUUUUCUCUUUUUGUCUGAACUUAUCGGCUCUUUUGUGAAUUUUUAUCGUGUGUAAUCGGACGUUUAGUUGUUGAGAUGUUUGUUUAGUAUUCAGCCGUCAGCGUUCAGCCAACUCAACAAUUCAACAUCUCAACAACUAAACAAUAAACAACUCAACAUUUAUUUCCUCAGGCUUACAGCCUUCCGACGGGAUGUGAUGCCUUUUCCCCAACGCUUCGCAUUGGGCUUUGAAUCUACUUACCUCAGAACAAGUACAUGUAUCA ...........((....(((.((((.((.((((......(((((((((..(((.((.((.((((((..((........))......))))))...)).)).)))...)))))))))...........................(((((...((((((........((((....(((((.(((((((((((((((((((((((.(((.......((....))........))).))))....)))))))))))))))))))...........................((((.....))))))))).((((...(....)...))))..))))))))))..)))))..............)))).)).)))).)))....)) (-88.16) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGAUACAUGUACUUGUUCUGAGGUAAGUAGAUUCAAAGCCCAAUGCGAAGCGUUGGGGAAAAGGCAUCACAUCCCGUCGGAAGGCUGUAAGCCUGAGGAAAUAAAUGUUGAGUUGUUUAUUGUUUAGUUGUUGAGAUGUUGAAUUGUUGAGUUGGCUGAACGCUGACGGCUGAAUACUAAACAAACAUCUCAACAACUAAACGUCCGAUUACACACGAUAAAAAUUCACAAAAGAGCCGAUAAGUUCAGACAAAAAGAGAAAAUCAAACAGCCAAAAAUAGGGAUAACAAGACAAAAAAUACCAAUUAUUAGGGAUUGUCCAUAACCACAUAACGACCGUCCUUUGCACUCACAAAAAGUUCAUUUAACAUACGAAAUGGA ((.((.(((.((((.((.((((................((((((((...))))))))......(((....(((((.......((((((..(.(((((........(((((.((.(((...((((((((((((((((((((....((((.(((.(((((........)))))....))).))))))))))))))))))))))))...)))...)).)))))....(((......)))........))))).).....((.....))..)))))).......))))).........................((((((.(((..............))).))))))))).)))).)).)))).))).)).))........... (-91.98) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 835 340 292 49 1 tfor:TF0821|5end_hypothetical_protein_879879..880109_POSITIVE 826 340 291 221 171 tfor:TF1187|5end_hypothetical_protein_1269625..1269855_POSITIVE 826 340 291 164 114 tfor:TF1186|5end_hypothetical_protein_1269568..1269798_POSITIVE 791 350 303 168 122 tfor:TF1183|5end_hypothetical_protein_1268391..1268621_POSITIVE 787 337 291 65 18 tfor:TF3107|5end_hypothetical_protein_3338459..3338689_POSITIVE 751 340 291 187 137 tfor:TF1184|5end_hypothetical_protein_1269004..1269234_POSITIVE 733 340 291 87 38 tfor:TF2191|5end_hypothetical_protein_2365934..2366164_POSITIVE 733 340 291 222 173 tfor:TF0823|5end_conserved_hypothetical_protein_880660..880890_POSITIVE 708 380 333 81 34 tfor:TF0844|5end_transposase,_IS3_family_895204..895434_POSITIVE 704 340 292 49 1 tfor:TF0822|5end_hypothetical_protein_880487..880717_POSITIVE 694 337 291 73 27 tfor:TF0679|5end_hypothetical_protein_721155..721385_POSITIVE 691 146 101 231 186 tfor:TF0599|5end_hypothetical_protein_629835..630065_POSITIVE 680 340 291 170 121 tfor:TF0828|5end_hypothetical_protein_887090..887320_POSITIVE 632 340 291 160 113 tfor:TF2824|5end_nucleoside-transporting_protein_nupG_3014701..3014931_POSITIVE 609 337 291 182 137 tfor:TF2478|5end_hypothetical_protein_2655375..2655605_POSITIVE 533 250 205 166 111 tfor:TF0449|5end_transposase_467891..468121_POSITIVE 530 337 291 69 21 tfor:TF1716|5end_hypothetical_protein_1837442..1837672_POSITIVE 523 340 291 192 144 tfor:TF2036|5end_possible_pyruvate_formate-lyase_activating_enzyme_2196302..2196532_POSITIVE 502 350 303 102 40 tfor:TF0538|5end_hypothetical_protein_566891..567121_POSITIVE 502 350 303 95 33 tfor:TF0539|5end_hypothetical_protein_566884..567114_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 870 340 291 117 67 tfor:TF2547|3end_hypothetical_protein_2730947..2731097_POSITIVE 826 340 291 79 29 tfor:TF1185|3end_hypothetical_protein_1269563..1269713_POSITIVE 787 337 291 134 87 tfor:TF3106|3end_hypothetical_protein_3338608..3338758_POSITIVE 716 350 303 49 3 tfor:TF1182|3end_hypothetical_protein_1268938..1269088_POSITIVE 663 350 309 40 1 tfor:TF0820|3end_hypothetical_protein_879942..880092_POSITIVE 533 250 205 106 51 tfor:TF0448|3end_hypothetical_protein_467911..468061_POSITIVE 477 236 191 59 7 tfor:TF1816|3end_hypothetical_protein_1957974..1958124_POSITIVE 477 236 191 76 24 tfor:TF1817|3end_hypothetical_protein_1957991..1958141_POSITIVE 468 328 286 132 88 tfor:TF1274|3end_hypothetical_protein_1357895..1358045_POSITIVE 452 347 303 42 3 tfor:TF0370|3end_hypothetical_protein_386317..386467_POSITIVE 447 337 291 141 93 tfor:TF1405|3end_conserved_hypothetical_protein_1481819..1481969_POSITIVE 428 313 286 151 122 tfor:TF0678|3end_hypothetical_protein_721335..721485_POSITIVE 400 350 302 61 9 tfor:TF2840|3end_hypothetical_protein_3031289..3031439_POSITIVE 395 250 201 62 6 tfor:TF2832|3end_possible_proton/sodium:glutamate_symporter_protein_3023939..3024089_POSITIVE 375 260 216 45 6 tfor:TF2839|3end_ATP-dependent_exodeoxyribonuclease_3031035..3031185_POSITIVE 371 356 316 58 7 tfor:TF2442|3end_heat_shock_protein_60_2620271..2620421_POSITIVE 365 66 32 80 43 tfor:TF1807|3end_thioesterase_family_protein_1949425..1949575_POSITIVE 364 245 210 97 58 tfor:TF0533|3end_hypothetical_protein_559519..559669_POSITIVE 359 260 228 33 1 tfor:TF1657|3end_lauroyl/myristoyl_acyltransferase_1773407..1773557_POSITIVE 358 249 203 49 1 tfor:TF1715|3end_thermolysin;_zinc_metalloprotease_1837202..1837352_POSITIVE