Origin IGS: ttcatcccccttggtaaattcgtcgttgatgtttgagccggtgaaggacagcgatattttgccgtcggtataaaacgtatcgtcgattttttatatgtcggaagttgtttttgttttctcgtagatgaattttccgctta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taagcggaaaattcatctacgagaaaacaaaaacaacttccgacatataaaaaatcgacgatacgttttataccgacggcaaaatatcgctgtccttcaccggctcaaacatcaacgacgaatttaccaagggggatgaa Mask Tandem Repeat Region ================================================ ttcatcccccttggtaaattcgtcgttgatgtttgagccggtgaaggacagcgatattttgccgtcggtataaaacgtatcgtcgattttttatatgtcggaagttgtttttgttttctcgtagatgaattttccgctta Find is-nt database================================================ Query_seq: TF0431:TF0432|TF0431:TF0432:hypothetical protein:3-deoxy-manno-octulosonate cytidylyltransferase:->->:452531..452670 140 ttcatcccccttggtaaattcgtcgttgatgtttgagccggtgaaggacagcgatattttgccgtcggtataaaacgtatcgtcgattttttatatgtcggaagttgtttttgttttctcgtagatgaattttccgctta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF0431:TF0432|TF0431:TF0432:hypothetical protein:3-deoxy-manno-octulosonate cytidylyltransferase:->->:452531..452670 140 ttcatcccccttggtaaattcgtcgttgatgtttgagccggtgaaggacagcgatattttgccgtcggtataaaacgtatcgtcgattttttatatgtcggaagttgtttttgttttctcgtagatgaattttccgctta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF0431:TF0432|TF0431:TF0432:hypothetical protein:3-deoxy-manno-octulosonate cytidylyltransferase:->->:452531..452670 140 ttcatcccccttggtaaattcgtcgttgatgtttgagccggtgaaggacagcgatattttgccgtcggtataaaacgtatcgtcgattttttatatgtcggaagttgtttttgttttctcgtagatgaattttccgctta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ttcatcccccttggtaaattcgtcgttgatgtttgagccggtgaaggacagcgatattttgccgtcggtataaaacgtatcgtcgattttttatatgtcggaagttgtttttgttttctcgtagatgaattttccgctta Predict ORF larger than 30AA ================================================ Protein_Len: 43 Strand: - Start: 5 End: 130 .... * G G Q Y I R R Q H K L R H L V A I N Q R R Y L V Y R R N K I H R F N N K N E R L H M .......... ttcatcccccttggtaaattcgtcgttgatgtttgagccggtgaaggacagcgatattttgccgtcggtataaaacgtatcgtcgattttttatatgtcggaagttgtttttgttttctcgtagatgaattttccgctta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ttcatcccccttggtaaattcgtcgttgatgtttgagccggtgaaggacagcgatattttgccgtcggtataaaacgtatcgtcgattttttatatgtcggaagttgtttttgttttctcgtagatgaattttccgctta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF0431:TF0432|TF0431:TF0432:hypothetical protein:3-deoxy-manno-octulosonate cytidylyltransferase:->->:452531..452670 140 ttcatcccccttggtaaattcgtcgttgatgtttgagccggtgaaggacagcgatattttgccgtcggtataaaacgtatcgtcgattttttatatgtcggaagttgtttttgttttctcgtagatgaattttccgctta Intra-Species Hit: Count: 1 Min: 1 Max: 140 Len: 140 Subject: tfor_TF0431_TF0432|hypothetical protein:3-deoxy-manno-octulosonate cytidylyltransferase|POSITIVE:POSITIVE|[452531, 452670]|140 HSP 1 e-value: 5.0E-74 bit: 278.0 Len: 140 Query Start:1 Query End:140 Subject Strand: POSITIVE Subject Start: 1 Subject End: 140 ttcatcccccttggtaaattcgtcgttgatgtttgagccggtgaaggacagcgatattttgccgtcggtataaaacgtatcgtcgattttttatatgtcggaagttgtttttgttttctcgtagatgaattttccgctta ttcatcccccttggtaaattcgtcgttgatgtttgagccggtgaaggacagcgatattttgccgtcggtataaaacgtatcgtcgattttttatatgtcggaagttgtttttgttttctcgtagatgaattttccgctta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUCAUCCCCCUUGGUAAAUUCGUCGUUGAUGUUUGAGCCGGUGAAGGACAGCGAUAUUUUGCCGUCGGUAUAAAACGUAUCGUCGAUUUUUUAUAUGUCGGAAGUUGUUUUUGUUUUCUCGUAGAUGAAUUUUCCGCUUA ............((.(((((((((....(((...((((....(((.(((..((((((.....((.((((((.....)))))).)).........))))))...))).)))..))))...))).))))))))).))..... (-29.44) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAGCGGAAAAUUCAUCUACGAGAAAACAAAAACAACUUCCGACAUAUAAAAAAUCGACGAUACGUUUUAUACCGACGGCAAAAUAUCGCUGUCCUUCACCGGCUCAAACAUCAACGACGAAUUUACCAAGGGGGAUGAA .....((.((((((.((.....))...............(((...(((((((.(((...)))...)))))))..((((((........))))))......)))................)))))).))............ (-19.60) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 446 69 21 210 144 tfor:TF1581|5end_hypothetical_protein_1689199..1689429_POSITIVE 430 69 21 219 181 tfor:TF0250|5end_glycosyltransferase_Family_2_protein_279176..279406_POSITIVE 405 68 21 60 6 tfor:TF2007|5end_uracil_phosphoribosyltransferase_2158591..2158821_POSITIVE 398 64 21 87 41 tfor:TF1740|5end_hypothetical_protein_1868645..1868875_POSITIVE 384 69 21 70 25 tfor:TF2990|5end_nicotinate-nucleotide_pyrophosphorylase_3205464..3205694_POSITIVE 378 70 21 187 154 tfor:TF0249|5end_3-hydroxymyristoyl/3-hydroxydecanoyl-(acyl_carrier_protein)_dehydratase_278808..279038_POSITIVE 377 69 21 53 7 tfor:TF2322|5end_conserved_hypothetical_protein;_possible_lipoprotein_2482743..2482973_POSITIVE 363 68 21 61 1 tfor:TF0826|5end_primosomal_protein_N'_883751..883981_POSITIVE 361 88 46 48 6 tfor:TF3022|5end_chorismate_synthase_3246136..3246366_POSITIVE 360 79 33 177 137 tfor:TF2502|5end_conserved_hypothetical_protein_2678103..2678333_POSITIVE 359 69 22 132 101 tfor:TF0992|5end_hypothetical_protein_1048251..1048481_POSITIVE 357 73 34 196 165 tfor:TF2368|5end_uridine_phosphorylase_2533666..2533896_POSITIVE 355 68 21 80 33 tfor:TF3114|5end_hypothetical_protein_3349134..3349364_POSITIVE 355 68 21 60 13 tfor:TF3113|5end_hypothetical_protein_3349114..3349344_POSITIVE 352 69 21 182 130 tfor:TF1179|5end_two-component_sensor_histidine_kinase_1266057..1266287_POSITIVE 349 69 21 169 123 tfor:TF2395|5end_conserved_hypothetical_protein_2566532..2566762_POSITIVE 347 64 21 99 49 tfor:TF2378|5end_peptide_chain_release_factor_1_2542682..2542912_POSITIVE 345 68 21 84 22 tfor:TF2434|5end_conserved_hypothetical_protein_2613969..2614199_POSITIVE 345 69 21 62 4 tfor:TF2213|5end_glycyl-tRNA_synthetase_2385319..2385549_POSITIVE 344 70 22 142 83 tfor:TF0473|5end_cation_or_acridine_efflux_membrane-fusion_protein_491047..491277_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 363 68 21 80 20 tfor:TF0825|3end_hypothetical_protein_883850..884000_POSITIVE 359 69 22 135 104 tfor:TF0991|3end_probable_NAD(FAD)-dependent_dehydrogenase_1048334..1048484_POSITIVE 349 69 21 147 101 tfor:TF2394|3end_integrase/recombinase_XerD_2566590..2566740_POSITIVE 345 68 21 78 16 tfor:TF2433|3end_possible_UDP-2,3-diacylglucosamine_hydrolase_2614043..2614193_POSITIVE 344 70 22 72 13 tfor:TF0472|3end_multidrug_resistance_protein,_AcrB/AcrD/AcrF_family_(cation_efflux_pump)_491057..491207_POSITIVE 342 77 37 75 31 tfor:TF0287|3end_peptidase,_M23_family_317467..317617_POSITIVE 341 77 31 83 37 tfor:TF2676|3end_conserved_hypothetical_protein_2850831..2850981_POSITIVE 338 86 45 131 94 tfor:TF2368|3end_uridine_phosphorylase_2534618..2534768_POSITIVE 334 54 11 110 63 tfor:TF2640|3end_hypothetical_protein_2809533..2809683_POSITIVE 334 66 23 121 83 tfor:TF2454|3end_D-alanyl-D-alanine_dipeptidase_2632025..2632175_POSITIVE 334 69 22 141 93 tfor:TF2135|3end_conserved_hypothetical_protein_2311635..2311785_POSITIVE 333 53 13 69 24 tfor:TF1498|3end_GTP-binding_protein_1598490..1598640_POSITIVE 332 46 2 151 101 tfor:TF2501|3end_DNA_mismatch_repair_protein_2678183..2678333_POSITIVE 329 68 21 141 80 tfor:TF0121|3end_hypothetical_protein_133217..133367_POSITIVE 328 69 24 43 1 tfor:TF2989|3end_conserved_hypothetical_protein_3205517..3205667_POSITIVE 326 80 37 88 31 tfor:TF1683|3end_conserved_hypothetical_protein_1796511..1796661_POSITIVE 325 54 11 83 45 tfor:TF0982|3end_conserved_hypothetical_protein_1039474..1039624_POSITIVE 322 68 22 127 72 tfor:TF1049|3end_cobyrinic_acid_a,c-diamide_synthase_1111539..1111689_POSITIVE 317 70 21 71 9 tfor:TF0235|3end_hypothetical_protein_261849..261999_POSITIVE 315 51 12 148 111 tfor:TF0584|3end_phosphopantetheinyl_transferase_614455..614605_POSITIVE