Origin IGS: ggaataaggatgagagacctatgtcaatcatacctgcacgccttctatctgcaaaaagcgtgtatatagtgctttcttgtggtgacttagatcgcatgtttttacacatttttttttcgtaaatatacggagtcttcctttgtattgccaaataaaaagtgtaaaaaagatttccttctttgattttttaatatttatttgatgatatcaattta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taaattgatatcatcaaataaatattaaaaaatcaaagaaggaaatcttttttacactttttatttggcaatacaaaggaagactccgtatatttacgaaaaaaaaatgtgtaaaaacatgcgatctaagtcaccacaagaaagcactatatacacgctttttgcagatagaaggcgtgcaggtatgattgacataggtctctcatccttattcc Mask Tandem Repeat Region ================================================ ggaataaggatgagagacctatgtcaatcatacctgcacgccttctatctgcaaaaagcgtgtatatagtgctttcttgtggtgacttagatcgcatgtttttacacatttttttttcgtaaatatacggagtcttcctttgtattgccaaataaaaagtgtaaaaaagatttccttctttgattttttaatatttatttgatgatatcaattta Find is-nt database================================================ Query_seq: TF0399:TF0401|TF0399:TF0401:hypothetical protein:hypothetical protein:->->:421268..421482 215 ggaataaggatgagagacctatgtcaatcatacctgcacgccttctatctgcaaaaagcgtgtatatagtgctttcttgtggtgacttagatcgcatgtttttacacatttttttttcgtaaatatacggagtcttcctttgtattgccaaataaaaagtgtaaaaaagatttccttctttgattttttaatatttatttgatgatatcaattta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF0399:TF0401|TF0399:TF0401:hypothetical protein:hypothetical protein:->->:421268..421482 215 ggaataaggatgagagacctatgtcaatcatacctgcacgccttctatctgcaaaaagcgtgtatatagtgctttcttgtggtgacttagatcgcatgtttttacacatttttttttcgtaaatatacggagtcttcctttgtattgccaaataaaaagtgtaaaaaagatttccttctttgattttttaatatttatttgatgatatcaattta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF0399:TF0401|TF0399:TF0401:hypothetical protein:hypothetical protein:->->:421268..421482 215 ggaataaggatgagagacctatgtcaatcatacctgcacgccttctatctgcaaaaagcgtgtatatagtgctttcttgtggtgacttagatcgcatgtttttacacatttttttttcgtaaatatacggagtcttcctttgtattgccaaataaaaagtgtaaaaaagatttccttctttgattttttaatatttatttgatgatatcaattta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ggaataaggatgagagacctatgtcaatcatacctgcacgccttctatctgcaaaaagcgtgtatatagtgctttcttgtggtgacttagatcgcatgtttttacacatttttttttcgtaaatatacggagtcttcctttgtattgccaaataaaaagtgtaaaaaagatttccttctttgattttttaatatttatttgatgatatcaattta Predict ORF larger than 30AA ================================================ Protein_Len: 33 Strand: + Start: 21 End: 119 .................... M S I I P A R L L S A K S V Y I V L S C G D L D R M F L H I F F S ................................................................................................ Protein_Len: 30 Strand: + Start: 91 End: 180 .......................................................................................... M A C F Y T F F F R K Y T E S S F V L P N K K C K K D F L L ................................... Protein_Len: 30 Strand: - Start: 105 End: 194 ........................................................................................................ V N K K R L Y V S D E K T N G F L F H L F S K R R Q N K L M ..................... Protein_Len: 56 Strand: - Start: 31 End: 198 .............................. V Q V G E I Q L F R T Y L A K K H H S L D C T K V C K K K T F I R L R G K Y Q W I F L T F F I E K K S K K I N M ................. Protein_Len: 60 Strand: - Start: 2 End: 184 . L S S L G I D I M G A R R R D A F L T Y I T S E Q P S K S R M N K C M K K E Y I Y P T K R Q I A L Y F T Y F L N G E K M ............................... ggaataaggatgagagacctatgtcaatcatacctgcacgccttctatctgcaaaaagcgtgtatatagtgctttcttgtggtgacttagatcgcatgtttttacacatttttttttcgtaaatatacggagtcttcctttgtattgccaaataaaaagtgtaaaaaagatttccttctttgattttttaatatttatttgatgatatcaattta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ggaataaggatgagagacctatgtcaatcatacctgcacgccttctatctgcaaaaagcgtgtatatagtgctttcttgtggtgacttagatcgcatgtttttacacatttttttttcgtaaatatacggagtcttcctttgtattgccaaataaaaagtgtaaaaaagatttccttctttgattttttaatatttatttgatgatatcaattta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF0399:TF0401|TF0399:TF0401:hypothetical protein:hypothetical protein:->->:421268..421482 215 ggaataaggatgagagacctatgtcaatcatacctgcacgccttctatctgcaaaaagcgtgtatatagtgctttcttgtggtgacttagatcgcatgtttttacacatttttttttcgtaaatatacggagtcttcctttgtattgccaaataaaaagtgtaaaaaagatttccttctttgattttttaatatttatttgatgatatcaattta Intra-Species Hit: Count: 1 Min: 1 Max: 215 Len: 215 Subject: tfor_TF0399_TF0401|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[421268,421482]|215 HSP 1 e-value: 1.0E-102 bit: 373.0 Len: 215 Query Start:1 Query End:215 Subject Strand: POSITIVE Subject Start: 1 Subject End: 215 ggaataaggatgagagacctatgtcaatcatacctgcacgccttctatctgcaaaaagcgtgtatatagtgctttcttgtggtgacttagatcgcatgtttttacacannnnnnnnncgtaaatatacggagtcttcctttgtattgccaaataaaaagtgtaaaaaagatttccttctttgattttttaatatttatttgatgatatcaattta ggaataaggatgagagacctatgtcaatcatacctgcacgccttctatctgcaaaaagcgtgtatatagtgctttcttgtggtgacttagatcgcatgtttttacacatttttttttcgtaaatatacggagtcttcctttgtattgccaaataaaaagtgtaaaaaagatttccttctttgattttttaatatttatttgatgatatcaattta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GGAAUAAGGAUGAGAGACCUAUGUCAAUCAUACCUGCACGCCUUCUAUCUGCAAAAAGCGUGUAUAUAGUGCUUUCUUGUGGUGACUUAGAUCGCAUGUUUUUACACAUUUUUUUUUCGUAAAUAUACGGAGUCUUCCUUUGUAUUGCCAAAUAAAAAGUGUAAAAAAGAUUUCCUUCUUUGAUUUUUUAAUAUUUAUUUGAUGAUAUCAAUUUA ((((..(((((((..(((....)))..)))).)))((((((................)))))).....((((..(((...(....)..)))..)))).(((((((((.(((((.(((.(((...((((((((.....))))))))))).))).))))))))))))))....))))....(((((...((((.......))))....))))).... (-46.89) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAUUGAUAUCAUCAAAUAAAUAUUAAAAAAUCAAAGAAGGAAAUCUUUUUUACACUUUUUAUUUGGCAAUACAAAGGAAGACUCCGUAUAUUUACGAAAAAAAAAUGUGUAAAAACAUGCGAUCUAAGUCACCACAAGAAAGCACUAUAUACACGCUUUUUGCAGAUAGAAGGCGUGCAGGUAUGAUUGACAUAGGUCUCUCAUCCUUAUUCC ....(((((((..........)))))))..........(((((.....((((((((((((((.((((..((((....(((....)))...))))..)))).)))))..))))))))).....((((((.((((......(....)..(((((.(((((((((((....)))))))))))...)))))..)))).)))))).....)))))..... (-43.60) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 303 46 2 85 43 tfor:TF0703|5end_conserved_hypothetical_protein_747252..747482_POSITIVE 301 54 35 26 7 tfor:TF0898|5end_hypothetical_protein_945366..945596_POSITIVE 296 50 2 231 176 tfor:TF2373|5end_UDP-3-O-acyl-GlcNAc_deacetylase_2538344..2538574_POSITIVE 295 63 21 160 110 tfor:TF2489|5end_hypothetical_protein_2664418..2664648_POSITIVE 292 71 34 148 110 tfor:TF2272|5end_conserved_hypothetical_protein_2436156..2436386_POSITIVE 291 48 1 61 23 tfor:TF1215|5end_haloacid_dehalogenase-like_hydrolase_1302510..1302740_POSITIVE 286 149 101 214 155 tfor:TF3065|5end_enolase_3291055..3291285_POSITIVE 285 70 27 226 180 tfor:TF0160|5end_conserved_hypothetical_protein;_possible_Fic_family_protein_170327..170557_POSITIVE 284 54 36 19 1 tfor:TF0897|5end_hypothetical_protein_945359..945589_POSITIVE 283 79 33 229 164 tfor:TF0399|5end_hypothetical_protein_419775..420005_POSITIVE 281 100 51 64 19 tfor:TF1774|5end_arylsulfatase_precursor_1911426..1911656_POSITIVE 277 65 22 203 162 tfor:TF0231|5end_conserved_hypothetical_protein_257804..258034_POSITIVE 274 148 109 66 22 tfor:TF2133|5end_aspartate-semialdehyde_dehydrogenase_2310275..2310505_POSITIVE 273 53 16 36 1 tfor:TF0754|5end_GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-redu_ctase_(GDP-L-fucose_synthetase)_801631..801861_POSITIVE 273 60 13 73 26 tfor:TF0107|5end_ion_channel_protein_113913..114143_POSITIVE 272 87 44 219 162 tfor:TF2991|5end_conserved_hypothetical_protein_3206316..3206546_POSITIVE 268 80 33 193 147 tfor:TF2820|5end_argininosuccinate_synthetase_3009876..3010106_POSITIVE 267 42 7 206 165 tfor:TF3149|5end_conserved_hypothetical_protein_3384424..3384654_POSITIVE 267 63 23 171 132 tfor:TF1264|5end_possible_transposase_1351290..1351520_POSITIVE 264 95 58 157 117 tfor:TF2736|5end_methylglyoxal_synthase_2917917..2918147_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 352 95 58 139 93 tfor:TF0343|3end_hypothetical_protein_366923..367073_POSITIVE 295 63 21 140 90 tfor:TF2488|3end_hypothetical_protein_2664478..2664628_POSITIVE 292 71 34 99 61 tfor:TF2271|3end_conserved_hypothetical_protein_2436187..2436337_POSITIVE 290 95 58 144 104 tfor:TF2169|3end_hypothetical_protein_2345354..2345504_POSITIVE 290 72 33 141 80 tfor:TF0499|3end_hypothetical_protein_526651..526801_POSITIVE 286 149 101 63 4 tfor:TF3064|3end_oxidoreductase,_aldo/keto_reductase_family_3290984..3291134_POSITIVE 273 53 16 38 3 tfor:TF0752|3end_nucleotide-binding_protein;_MAF-like_protein_801713..801863_POSITIVE 271 60 11 147 92 tfor:TF1292|3end_sensor_histidine_protein_kinase_1374554..1374704_POSITIVE 267 63 23 136 97 tfor:TF1263|3end_DNA-mismatch_repair_protein_1351335..1351485_POSITIVE 267 50 1 60 8 tfor:TF0337|3end_hypothetical_protein_360711..360861_POSITIVE 264 95 58 88 48 tfor:TF2735|3end_hypothetical_protein_2917928..2918078_POSITIVE 262 78 39 38 1 tfor:TF2563|3end_30S_ribosomal_protein_S17_2739679..2739829_POSITIVE 255 60 19 101 57 tfor:TF3126|3end_glycosyltransferase,_group_2_family_3361685..3361835_POSITIVE 255 96 58 143 93 tfor:TF0361|3end_hypothetical_protein_379810..379960_POSITIVE 255 96 58 143 93 tfor:TF0349|3end_hypothetical_protein_370719..370869_POSITIVE 252 75 31 57 5 tfor:TF2708|3end_DNA_gyrase_subunit_B_2882199..2882349_POSITIVE 249 77 34 50 5 tfor:TF0095|3end_methyltransferase_106845..106995_POSITIVE 248 96 57 67 27 tfor:TF0599|3end_hypothetical_protein_630130..630280_POSITIVE 246 87 42 77 21 tfor:TF3017|3end_hypothetical_protein_3239903..3240053_POSITIVE 246 100 51 82 23 tfor:TF2120|3end_holliday_junction_endonuclease_2292760..2292910_POSITIVE