Origin IGS: ttattgatgtttatgtttaaaatacggcgtaaaagtacggatttattgtttgaacaaaatgtaaaaaaagtttcatttttggaatggttggtcgttatagactgttcatgaacttgggatatgaaggcatatctcccgaagtatgcaaaaaaaaaattgaaagtgaattgtctgtcgtcagtgaagatggatcgaattttcgatagagtatttatggataattttgttgaaaaacgaaacgtgatattcttaaaaaaatagaaaaggcggcctca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tgaggccgccttttctatttttttaagaatatcacgtttcgtttttcaacaaaattatccataaatactctatcgaaaattcgatccatcttcactgacgacagacaattcactttcaattttttttttgcatacttcgggagatatgccttcatatcccaagttcatgaacagtctataacgaccaaccattccaaaaatgaaactttttttacattttgttcaaacaataaatccgtacttttacgccgtattttaaacataaacatcaataa Mask Tandem Repeat Region ================================================ ttattgatgtttatgtttaaaatacggcgtaaaagtacggatttattgtttgaacaaaatgtaaaaaaagtttcatttttggaatggttggtcgttatagactgttcatgaacttgggatatgaaggcatatctcccgaagtatgcaaaaaaaaaattgaaagtgaattgtctgtcgtcagtgaagatggatcgaattttcgatagagtatttatggataattttgttgaaaaacgaaacgtgatattcttaaaaaaatagaaaaggcggcctca Find is-nt database================================================ Query_seq: TF0391:TF0393|TF0391:TF0393:hypothetical protein:conserved hypothetical protein:->->:412149..412423 275 ttattgatgtttatgtttaaaatacggcgtaaaagtacggatttattgtttgaacaaaatgtaaaaaaagtttcatttttggaatggttggtcgttatagactgttcatgaacttgggatatgaaggcatatctcccgaagtatgcaaaaaaaaaattgaaagtgaattgtctgtcgtcagtgaagatggatcgaattttcgatagagtatttatggataattttgttgaaaaacgaaacgtgatattcttaaaaaaatagaaaaggcggcctca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF0391:TF0393|TF0391:TF0393:hypothetical protein:conserved hypothetical protein:->->:412149..412423 275 ttattgatgtttatgtttaaaatacggcgtaaaagtacggatttattgtttgaacaaaatgtaaaaaaagtttcatttttggaatggttggtcgttatagactgttcatgaacttgggatatgaaggcatatctcccgaagtatgcaaaaaaaaaattgaaagtgaattgtctgtcgtcagtgaagatggatcgaattttcgatagagtatttatggataattttgttgaaaaacgaaacgtgatattcttaaaaaaatagaaaaggcggcctca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF0391:TF0393|TF0391:TF0393:hypothetical protein:conserved hypothetical protein:->->:412149..412423 275 ttattgatgtttatgtttaaaatacggcgtaaaagtacggatttattgtttgaacaaaatgtaaaaaaagtttcatttttggaatggttggtcgttatagactgttcatgaacttgggatatgaaggcatatctcccgaagtatgcaaaaaaaaaattgaaagtgaattgtctgtcgtcagtgaagatggatcgaattttcgatagagtatttatggataattttgttgaaaaacgaaacgtgatattcttaaaaaaatagaaaaggcggcctca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ttattgatgtttatgtttaaaatacggcgtaaaagtacggatttattgtttgaacaaaatgtaaaaaaagtttcatttttggaatggttggtcgttatagactgttcatgaacttgggatatgaaggcatatctcccgaagtatgcaaaaaaaaaattgaaagtgaattgtctgtcgtcagtgaagatggatcgaattttcgatagagtatttatggataattttgttgaaaaacgaaacgtgatattcttaaaaaaatagaaaaggcggcctca Predict ORF larger than 30AA ================================================ Protein_Len: 35 Strand: + Start: 4 End: 108 ... M M F M F K I R R K S T D L L F E Q N V K K V S F L E W L V V I D C S ....................................................................................................................................................................... Protein_Len: 43 Strand: + Start: 75 End: 203 .......................................................................... M F G M V G R Y R L F M N L G Y E G I S P E V C K K K I E S E L S V V S E D G S N F R ........................................................................ Protein_Len: 46 Strand: + Start: 121 End: 258 ........................................................................................................................ M K A Y L P K Y A K K K L K V N C L S S V K M D R I F D R V F M D N F V E K R N V I F L K K ................. Protein_Len: 38 Strand: - Start: 98 End: 211 ................................................................................................. L S N M F K P Y S P M D G S T H L F F I S L S N D T T L S S P D F K R Y L M ................................................................ Protein_Len: 60 Strand: - Start: 3 End: 245 .. F T E N K S H N T T I S Q E H V Q S I F A Y R G F Y A F F F N F T F Q R D D T F I S R I K S L T N I S L K T S F R F T M .............................. ttattgatgtttatgtttaaaatacggcgtaaaagtacggatttattgtttgaacaaaatgtaaaaaaagtttcatttttggaatggttggtcgttatagactgttcatgaacttgggatatgaaggcatatctcccgaagtatgcaaaaaaaaaattgaaagtgaattgtctgtcgtcagtgaagatggatcgaattttcgatagagtatttatggataattttgttgaaaaacgaaacgtgatattcttaaaaaaatagaaaaggcggcctca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ttattgatgtttatgtttaaaatacggcgtaaaagtacggatttattgtttgaacaaaatgtaaaaaaagtttcatttttggaatggttggtcgttatagactgttcatgaacttgggatatgaaggcatatctcccgaagtatgcaaaaaaaaaattgaaagtgaattgtctgtcgtcagtgaagatggatcgaattttcgatagagtatttatggataattttgttgaaaaacgaaacgtgatattcttaaaaaaatagaaaaggcggcctca Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF0391:TF0393|TF0391:TF0393:hypothetical protein:conserved hypothetical protein:->->:412149..412423 275 ttattgatgtttatgtttaaaatacggcgtaaaagtacggatttattgtttgaacaaaatgtaaaaaaagtttcatttttggaatggttggtcgttatagactgttcatgaacttgggatatgaaggcatatctcccgaagtatgcaaaaaaaaaattgaaagtgaattgtctgtcgtcagtgaagatggatcgaattttcgatagagtatttatggataattttgttgaaaaacgaaacgtgatattcttaaaaaaatagaaaaggcggcctca Intra-Species Hit: Count: 1 Min: 1 Max: 275 Len: 275 Subject: tfor_TF0391_TF0393|hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[412149,412423]|275 HSP 1 e-value: 1.0E-111 bit: 402.0 Len: 275 Query Start:1 Query End:275 Subject Strand: POSITIVE Subject Start: 1 Subject End: 275 ttattgatgtttatgtttaaaatacggcgtaaaagtacggatttattgtttgaacaaaatgtnnnnnnngtttcatttttggaatggttggtcgttatagactgttcatgaacttgggatatgaaggcatatctcccgaagtatgcnnnnnnnnnnttgaaagtgaattgtctgtcgtcagtgaagatggatcgaattttcgatagagtatttatggataattttgttgaaaaacgaaacgtgatattcttnnnnnnntagaaaaggcggcctca ttattgatgtttatgtttaaaatacggcgtaaaagtacggatttattgtttgaacaaaatgtaaaaaaagtttcatttttggaatggttggtcgttatagactgttcatgaacttgggatatgaaggcatatctcccgaagtatgcaaaaaaaaaattgaaagtgaattgtctgtcgtcagtgaagatggatcgaattttcgatagagtatttatggataattttgttgaaaaacgaaacgtgatattcttaaaaaaatagaaaaggcggcctca Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUAUUGAUGUUUAUGUUUAAAAUACGGCGUAAAAGUACGGAUUUAUUGUUUGAACAAAAUGUAAAAAAAGUUUCAUUUUUGGAAUGGUUGGUCGUUAUAGACUGUUCAUGAACUUGGGAUAUGAAGGCAUAUCUCCCGAAGUAUGCAAAAAAAAAAUUGAAAGUGAAUUGUCUGUCGUCAGUGAAGAUGGAUCGAAUUUUCGAUAGAGUAUUUAUGGAUAAUUUUGUUGAAAAACGAAACGUGAUAUUCUUAAAAAAAUAGAAAAGGCGGCCUCA (((((((((((((((((........))))))))...((((((...((((....))))..............((((((((..(.((((..((((......))))..))))..((((((((((((....))))..)))).))))..............)..))))))))..))))))))))))))).((.((.(((..(((((...((((((((.(((.....(((((((....))))))))))))))))))..........)))))..))))))). (-60.02) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGAGGCCGCCUUUUCUAUUUUUUUAAGAAUAUCACGUUUCGUUUUUCAACAAAAUUAUCCAUAAAUACUCUAUCGAAAAUUCGAUCCAUCUUCACUGACGACAGACAAUUCACUUUCAAUUUUUUUUUUGCAUACUUCGGGAGAUAUGCCUUCAUAUCCCAAGUUCAUGAACAGUCUAUAACGACCAACCAUUCCAAAAAUGAAACUUUUUUUACAUUUUGUUCAAACAAUAAAUCCGUACUUUUACGCCGUAUUUUAAACAUAAACAUCAAUAA ...(((.............................(((((((((((.........................(((((....))))).................((((..((((....(((........)))...((((.((((..((((....))))))))))))...))))..))))..................)))))))))))......(((..((((((.....))))))...))).......)))......................... (-31.90) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 339 217 172 66 22 tfor:TF0388|5end_conserved_hypothetical_protein;_possible_integral_membrane_protein_408002..408232_POSITIVE 320 220 174 93 47 tfor:TF0892|5end_conserved_hypothetical_protein_940836..941066_POSITIVE 306 208 164 134 92 tfor:TF1203|5end_L-asparaginase_I_1285232..1285462_POSITIVE 303 130 82 78 34 tfor:TF1275|5end_transcription-repair_coupling_factor_1357866..1358096_POSITIVE 300 207 161 121 73 tfor:TF2747|5end_heat_shock_protein,_HSP90_family_2928279..2928509_POSITIVE 297 59 19 171 129 tfor:TF2933|5end_30S_ribosomal_protein_S21_3137344..3137574_POSITIVE 297 198 157 92 57 tfor:TF1580|5end_conserved_hypothetical_protein;_possible_fibronectin_domain_containing_protein_1687187..1687417_POSITIVE 294 128 81 230 173 tfor:TF2001|5end_conserved_hypothetical_protein_2155384..2155614_POSITIVE 287 130 81 92 36 tfor:TF0964|5end_conserved_hypothetical_protein_1012955..1013185_POSITIVE 283 140 91 122 65 tfor:TF2968|5end_hypothetical_protein_3178170..3178400_POSITIVE 283 208 167 203 159 tfor:TF0902|5end_methyltransferase_947237..947467_POSITIVE 282 194 155 48 16 tfor:TF2344|5end_ATP/GTP_binding_protein_2507422..2507652_POSITIVE 281 177 131 158 100 tfor:TF2275|5end_conserved_hypothetical_protein_2436934..2437164_POSITIVE 280 137 91 106 66 tfor:TF2433|5end_possible_UDP-2,3-diacylglucosamine_hydrolase_2613205..2613435_POSITIVE 277 237 192 226 181 tfor:TF0392|5end_hypothetical_protein_412335..412565_POSITIVE 274 194 163 82 41 tfor:TF2006|5end_uracil_permease_2157279..2157509_POSITIVE 272 218 172 84 36 tfor:TF1619|5end_riboflavin_synthase,_beta_subunit_1733948..1734178_POSITIVE 271 210 163 217 169 tfor:TF0975|5end_hypothetical_protein_1023994..1024224_POSITIVE 269 117 71 163 102 tfor:TF2202|5end_hypothetical_protein_2378682..2378912_POSITIVE 268 123 81 187 149 tfor:TF1229|5end_hypothetical_protein_1314619..1314849_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 298 208 164 47 7 tfor:TF0242|3end_conserved_hypothetical_protein_274069..274219_POSITIVE 297 198 157 99 64 tfor:TF1579|3end_hypothetical_protein_1687274..1687424_POSITIVE 287 130 81 92 36 tfor:TF0963|3end_acyltransferase_1013035..1013185_POSITIVE 282 194 155 48 16 tfor:TF2343|3end_ribosome_recycling_factor_2507502..2507652_POSITIVE 281 177 131 130 72 tfor:TF2274|3end_conserved_hypothetical_protein_2436986..2437136_POSITIVE 281 139 91 151 98 tfor:TF0271|3end_oxidase/dehydrogenase_298530..298680_POSITIVE 267 130 81 53 7 tfor:TF2205|3end_conserved_hypothetical_protein;_possible_transcriptional_regulator_2380010..2380160_POSITIVE 265 139 102 123 78 tfor:TF2247|3end_transfer_region-related_protein,_TraA_2422625..2422775_POSITIVE 263 126 81 68 22 tfor:TF0743|3end_conserved_hypothetical_protein_790730..790880_POSITIVE 260 129 83 101 50 tfor:TF2808|3end_bacterial_sugar_transferase_2999909..3000059_POSITIVE 260 210 163 81 35 tfor:TF1821|3end_cation_efflux_system_protein_1967645..1967795_POSITIVE 260 133 98 46 1 tfor:TF0982|3end_conserved_hypothetical_protein_1039474..1039624_POSITIVE 257 128 81 53 4 tfor:TF0141|3end_exodeoxyribonuclease_VII,_large_subunit_149830..149980_POSITIVE 256 199 160 63 25 tfor:TF1471|3end_conserved_hypothetical_protein_1565521..1565671_POSITIVE 255 218 174 95 53 tfor:TF2696|3end_oxidoreductase,_short_chain_dehydrogenase/reductase_family_2871611..2871761_POSITIVE 252 129 81 111 54 tfor:TF2899|3end_conserved_hypothetical_protein_3088345..3088495_POSITIVE 252 145 112 47 2 tfor:TF0188|3end_membrane-associated_porT_protein_203249..203399_POSITIVE 251 123 84 36 1 tfor:TF1228|3end_conserved_hypothetical_protein_1314548..1314698_POSITIVE 250 209 163 70 20 tfor:TF2492|3end_NifU-related_protein_2667077..2667227_POSITIVE 250 208 168 62 13 tfor:TF1028|3end_1-acyl-sn-glycerol-3-phosphate_acyltransferase_1086775..1086925_POSITIVE