Origin IGS: ttttttgcttattccctccctgttaccaaatcacaggacgaaataattcagcgggcaaagatagcgatatttctctaataaatcaagctgaacgaacgagctgaccgaggacacgttaagcatgaagaaaacacgctaagcgttgtatccataatacaacttttttatctttgcatcgaaagaatatgatgaaacttaaaaacttttcatctgcttttatgatgcggaatttgaaaattatcccgtaccttt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aaaggtacgggataattttcaaattccgcatcataaaagcagatgaaaagtttttaagtttcatcatattctttcgatgcaaagataaaaaagttgtattatggatacaacgcttagcgtgttttcttcatgcttaacgtgtcctcggtcagctcgttcgttcagcttgatttattagagaaatatcgctatctttgcccgctgaattatttcgtcctgtgatttggtaacagggagggaataagcaaaaaa Mask Tandem Repeat Region ================================================ ttttttgcttattccctccctgttaccaaatcacaggacgaaataattcagcgggcaaagatagcgatatttctctaataaatcaagctgaacgaacgagctgaccgaggacacgttaagcatgaagaaaacacgctaagcgttgtatccataatacaacttttttatctttgcatcgaaagaatatgatgaaacttaaaaacttttcatctgcttttatgatgcggaatttgaaaattatcccgtaccttt Find is-nt database================================================ Query_seq: TF0377:TF0378|TF0377:TF0378:FtsK/SpoIIIE family cell division protein:aspartyl-tRNA synthetase:->->:397529..397780 252 ttttttgcttattccctccctgttaccaaatcacaggacgaaataattcagcgggcaaagatagcgatatttctctaataaatcaagctgaacgaacgagctgaccgaggacacgttaagcatgaagaaaacacgctaagcgttgtatccataatacaacttttttatctttgcatcgaaagaatatgatgaaacttaaaaacttttcatctgcttttatgatgcggaatttgaaaattatcccgtaccttt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF0377:TF0378|TF0377:TF0378:FtsK/SpoIIIE family cell division protein:aspartyl-tRNA synthetase:->->:397529..397780 252 ttttttgcttattccctccctgttaccaaatcacaggacgaaataattcagcgggcaaagatagcgatatttctctaataaatcaagctgaacgaacgagctgaccgaggacacgttaagcatgaagaaaacacgctaagcgttgtatccataatacaacttttttatctttgcatcgaaagaatatgatgaaacttaaaaacttttcatctgcttttatgatgcggaatttgaaaattatcccgtaccttt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF0377:TF0378|TF0377:TF0378:FtsK/SpoIIIE family cell division protein:aspartyl-tRNA synthetase:->->:397529..397780 252 ttttttgcttattccctccctgttaccaaatcacaggacgaaataattcagcgggcaaagatagcgatatttctctaataaatcaagctgaacgaacgagctgaccgaggacacgttaagcatgaagaaaacacgctaagcgttgtatccataatacaacttttttatctttgcatcgaaagaatatgatgaaacttaaaaacttttcatctgcttttatgatgcggaatttgaaaattatcccgtaccttt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ttttttgcttattccctccctgttaccaaatcacaggacgaaataattcagcgggcaaagatagcgatatttctctaataaatcaagctgaacgaacgagctgaccgaggacacgttaagcatgaagaaaacacgctaagcgttgtatccataatacaacttttttatctttgcatcgaaagaatatgatgaaacttaaaaacttttcatctgcttttatgatgcggaatttgaaaattatcccgtaccttt Predict ORF larger than 30AA ================================================ Protein_Len: 35 Strand: + Start: 82 End: 186 ................................................................................. M K L N E R A D R G H V K H E E N T L S V V S I I Q L F Y L C I E R I .................................................................. Protein_Len: 35 Strand: + Start: 147 End: 251 .................................................................................................................................................. M H N T T F L S L H R K N M M K L K N F S S A F M M R N L K I I P Y L . Protein_Len: 41 Strand: - Start: 119 End: 241 ...................................................................................................................... A H L F C A L R Q I W L V V K K D K C R F F I I F S L F K E D A K I I R F K F I M ........... Protein_Len: 30 Strand: - Start: 34 End: 123 ................................. L V F Y N L P C L Y R Y K E L L D L Q V F S S V S S V N L M ................................................................................................................................. ttttttgcttattccctccctgttaccaaatcacaggacgaaataattcagcgggcaaagatagcgatatttctctaataaatcaagctgaacgaacgagctgaccgaggacacgttaagcatgaagaaaacacgctaagcgttgtatccataatacaacttttttatctttgcatcgaaagaatatgatgaaacttaaaaacttttcatctgcttttatgatgcggaatttgaaaattatcccgtaccttt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: - Score: 82 Start: 58 End: 87 .........................................................CTTGATTTATTAGAGAAATATCGCTATCTT..................................................................................................................................................................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ttttttgcttattccctccctgttaccaaatcacaggacgaaataattcagcgggcaaagatagcgatatttctctaataaatcaagctgaacgaacgagctgaccgaggacacgttaagcatgaagaaaacacgctaagcgttgtatccataatacaacttttttatctttgcatcgaaagaatatgatgaaacttaaaaacttttcatctgcttttatgatgcggaatttgaaaattatcccgtaccttt Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 89 HP_score: -6.1 Tail_Score: -5.56264 Start: 142 End: 160 Full_Region: GAAAACACGCTAAGC GTTGTAT CCATA ATACAAC TTTTTTATCTTTGCA .............................................................................................................................................GTTGTATCCATAATACAAC............................................................................................ Find igs database================================================ Query_seq: TF0377:TF0378|TF0377:TF0378:FtsK/SpoIIIE family cell division protein:aspartyl-tRNA synthetase:->->:397529..397780 252 ttttttgcttattccctccctgttaccaaatcacaggacgaaataattcagcgggcaaagatagcgatatttctctaataaatcaagctgaacgaacgagctgaccgaggacacgttaagcatgaagaaaacacgctaagcgttgtatccataatacaacttttttatctttgcatcgaaagaatatgatgaaacttaaaaacttttcatctgcttttatgatgcggaatttgaaaattatcccgtaccttt Intra-Species Hit: Count: 1 Min: 1 Max: 252 Len: 252 Subject: tfor_TF0377_TF0378|FtsK/SpoIIIE family cell division protein:aspartyl-tRNA synthetase|POSITIVE:POSITIVE|[397529,397780]|252 HSP 1 e-value: 1.0E-140 bit: 500.0 Len: 252 Query Start:1 Query End:252 Subject Strand: POSITIVE Subject Start: 1 Subject End: 252 ttttttgcttattccctccctgttaccaaatcacaggacgaaataattcagcgggcaaagatagcgatatttctctaataaatcaagctgaacgaacgagctgaccgaggacacgttaagcatgaagaaaacacgctaagcgttgtatccataatacaacttttttatctttgcatcgaaagaatatgatgaaacttaaaaacttttcatctgcttttatgatgcggaatttgaaaattatcccgtaccttt ttttttgcttattccctccctgttaccaaatcacaggacgaaataattcagcgggcaaagatagcgatatttctctaataaatcaagctgaacgaacgagctgaccgaggacacgttaagcatgaagaaaacacgctaagcgttgtatccataatacaacttttttatctttgcatcgaaagaatatgatgaaacttaaaaacttttcatctgcttttatgatgcggaatttgaaaattatcccgtaccttt Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUUUUUGCUUAUUCCCUCCCUGUUACCAAAUCACAGGACGAAAUAAUUCAGCGGGCAAAGAUAGCGAUAUUUCUCUAAUAAAUCAAGCUGAACGAACGAGCUGACCGAGGACACGUUAAGCAUGAAGAAAACACGCUAAGCGUUGUAUCCAUAAUACAACUUUUUUAUCUUUGCAUCGAAAGAAUAUGAUGAAACUUAAAAACUUUUCAUCUGCUUUUAUGAUGCGGAAUUUGAAAAUUAUCCCGUACCUUU ......(((..(((....(((((.........))))).........((((((..(......(((.((....)).)))......)..)))))).)))..))).....((((((..(((.(((.((.......)).))).)))(((((((.....))))))).((((((..(((((((((.((((.((.(((((((..........))))))))).)))).)))))))))...)))))).......)).)))). (-52.40) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAAGGUACGGGAUAAUUUUCAAAUUCCGCAUCAUAAAAGCAGAUGAAAAGUUUUUAAGUUUCAUCAUAUUCUUUCGAUGCAAAGAUAAAAAAGUUGUAUUAUGGAUACAACGCUUAGCGUGUUUUCUUCAUGCUUAACGUGUCCUCGGUCAGCUCGUUCGUUCAGCUUGAUUUAUUAGAGAAAUAUCGCUAUCUUUGCCCGCUGAAUUAUUUCGUCCUGUGAUUUGGUAACAGGGAGGGAAUAAGCAAAAAA ...(((.((((((.........)))))).)))......((.(((((((..(....)..))))))).(((((((((...(((((((.......((((((((...))))))))...(((((..(((((((((.(((.((((......(((.....)))..)))).))).)))......))))))....))))))))))))..((..((((((........))))))..))......))))))))).))...... (-64.50) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 361 136 93 103 59 tfor:TF2296|5end_hypothetical_protein_2448892..2449122_POSITIVE 334 250 203 224 173 tfor:TF0988|5end_conserved_hypothetical_protein;_possible_nucleotidyltransferase_1044463..1044693_POSITIVE 325 110 61 54 2 tfor:TF2165|5end_hypothetical_protein_2341654..2341884_POSITIVE 322 139 97 77 28 tfor:TF0906|5end_conserved_hypothetical_protein_949675..949905_POSITIVE 321 180 134 46 2 tfor:TF3065|5end_enolase_3291055..3291285_POSITIVE 320 130 87 99 47 tfor:TF0964|5end_conserved_hypothetical_protein_1012955..1013185_POSITIVE 319 117 86 215 182 tfor:TF0746|5end_possible_DNA_polymerase_III_792261..792491_POSITIVE 317 108 64 81 37 tfor:TF2879|5end_tRNA_(guanine-N-1)-methyltransferase_3068254..3068484_POSITIVE 308 127 87 156 117 tfor:TF3108|5end_glutamine-hydrolyzing_carbamoyl-phosphate_synthase,_large_subunit_3338732..3338962_POSITIVE 308 149 101 217 175 tfor:TF2770|5end_conserved_hypothetical_protein_2956308..2956538_POSITIVE 303 150 103 187 130 tfor:TF0096|5end_tRNA-guanine_transglycosylase_(queuine_tRNA-ribosyltransferase)_106971..107201_POSITIVE 300 136 93 83 33 tfor:TF2677|5end_aspartate_transaminase_2850809..2851039_POSITIVE 300 129 84 118 70 tfor:TF0785|5end_biopolymer_transport_protein,_MotA/TolQ/ExbB_proton_channel_family_837810..838040_POSITIVE 299 126 86 147 95 tfor:TF0175|5end_hypothetical_protein_185585..185815_POSITIVE 297 128 88 109 56 tfor:TF2679|5end_conserved_hypothetical_protein;_possible_permease_2853225..2853455_POSITIVE 297 130 88 54 12 tfor:TF0489|5end_D-xylose-proton_symporter_511493..511723_POSITIVE 295 142 102 142 98 tfor:TF2276|5end_conserved_hypothetical_protein_2438226..2438456_POSITIVE 292 108 83 189 157 tfor:TF1958|5end_cytidylate_kinase_2108659..2108889_POSITIVE 290 138 93 54 18 tfor:TF2267|5end_lysozyme-related_protein_2434869..2435099_POSITIVE 286 135 97 66 28 tfor:TF2039|5end_possible_cell-cycle_regulation_histidine_triad_(HIT)_protein_2199318..2199548_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 326 248 203 88 34 tfor:TF2744|3end_methionyl-tRNA_formyltransferase_2927378..2927528_POSITIVE 320 130 87 99 47 tfor:TF0963|3end_acyltransferase_1013035..1013185_POSITIVE 318 97 51 135 84 tfor:TF0128|3end_hypothetical_protein_140556..140706_POSITIVE 308 127 87 80 41 tfor:TF3107|3end_hypothetical_protein_3338736..3338886_POSITIVE 305 76 31 130 80 tfor:TF0082|3end_transcriptional_regulator,_TetR_family_89264..89414_POSITIVE 300 129 84 109 61 tfor:TF0784|3end_possible_biopolymer_transport_protein_837881..838031_POSITIVE 299 126 86 82 30 tfor:TF0174|3end_hypothetical_protein_185600..185750_POSITIVE 297 128 88 128 75 tfor:TF2678|3end_GTP_cyclohydrolase_II_/3,4-dihydroxy-2-butanone-4-phoshate_synthase_2853324..2853474_POSITIVE 295 60 11 51 10 tfor:TF2687|3end_iron-regulated_ABC-type_transporter_membrane_component_2864962..2865112_POSITIVE 295 142 102 136 92 tfor:TF2275|3end_conserved_hypothetical_protein_2438300..2438450_POSITIVE 290 138 93 54 18 tfor:TF2266|3end_transfer_region-related_protein,_TraQ_2434949..2435099_POSITIVE 282 135 92 52 19 tfor:TF1972|3end_conserved_hypothetical_protein_2119415..2119565_POSITIVE 281 122 87 79 47 tfor:TF0599|3end_hypothetical_protein_630130..630280_POSITIVE 277 137 91 148 96 tfor:TF1282|3end_tRNA_delta-2_isopentenylpyrophosphate_transferase_1367431..1367581_POSITIVE 276 129 87 55 22 tfor:TF0727|3end_ABC_transporter,_ATP-binding_protein_780178..780328_POSITIVE 275 127 92 61 20 tfor:TF0475|3end_possible_transcriptional_regulator,_AraC_family_494825..494975_POSITIVE 274 100 51 150 112 tfor:TF2529|3end_nucleotidyl_transferase_family;_possible_mannose-1-phosphate_guanyltransferase_2711496..2711646_POSITIVE 274 116 84 43 10 tfor:TF1249|3end_phosphoglycerate_kinase_(cytosolic_phosphoglycerate_kinase)_1332356..1332506_POSITIVE 273 130 89 135 92 tfor:TF2208|3end_conserved_hypothetical_protein_2383988..2384138_POSITIVE 273 137 93 107 55 tfor:TF2152|3end_uronate_isomerase_2328061..2328211_POSITIVE