Origin IGS: atgggacgaactattataaatataagtatttcaaggtagttttattccattttgaaccttgaagaacatagttattgtcccctgttaaagggccattatcgtataggcgctgtgaataaactttgaatattgcaatcgtagatagaaaaaacaaaagaataaatcgtttcatatta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taatatgaaacgatttattcttttgttttttctatctacgattgcaatattcaaagtttattcacagcgcctatacgataatggccctttaacaggggacaataactatgttcttcaaggttcaaaatggaataaaactaccttgaaatacttatatttataatagttcgtcccat Mask Tandem Repeat Region ================================================ atgggacgaactattataaatataagtatttcaaggtagttttattccattttgaaccttgaagaacatagttattgtcccctgttaaagggccattatcgtataggcgctgtgaataaactttgaatattgcaatcgtagatagaaaaaacaaaagaataaatcgtttcatatta Find is-nt database================================================ Query_seq: TF0367:TF0368|TF0367:TF0368:eukaryotic-like metalloproteinase:hypothetical protein:->->:385365..385540 176 atgggacgaactattataaatataagtatttcaaggtagttttattccattttgaaccttgaagaacatagttattgtcccctgttaaagggccattatcgtataggcgctgtgaataaactttgaatattgcaatcgtagatagaaaaaacaaaagaataaatcgtttcatatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF0367:TF0368|TF0367:TF0368:eukaryotic-like metalloproteinase:hypothetical protein:->->:385365..385540 176 atgggacgaactattataaatataagtatttcaaggtagttttattccattttgaaccttgaagaacatagttattgtcccctgttaaagggccattatcgtataggcgctgtgaataaactttgaatattgcaatcgtagatagaaaaaacaaaagaataaatcgtttcatatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF0367:TF0368|TF0367:TF0368:eukaryotic-like metalloproteinase:hypothetical protein:->->:385365..385540 176 atgggacgaactattataaatataagtatttcaaggtagttttattccattttgaaccttgaagaacatagttattgtcccctgttaaagggccattatcgtataggcgctgtgaataaactttgaatattgcaatcgtagatagaaaaaacaaaagaataaatcgtttcatatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 atgggacgaactattataaatataagtatttcaaggtagttttattccattttgaaccttgaagaacatagttattgtcccctgttaaagggccattatcgtataggcgctgtgaataaactttgaatattgcaatcgtagatagaaaaaacaaaagaataaatcgtttcatatta Predict ORF larger than 30AA ================================================ Protein_Len: 52 Strand: - Start: 17 End: 172 ................ L Y L Y K L T T K N W K S G Q L V Y N N D G T L P G N D Y L R Q S Y V K F I A I T S L F F L L I F R K M .... atgggacgaactattataaatataagtatttcaaggtagttttattccattttgaaccttgaagaacatagttattgtcccctgttaaagggccattatcgtataggcgctgtgaataaactttgaatattgcaatcgtagatagaaaaaacaaaagaataaatcgtttcatatta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ atgggacgaactattataaatataagtatttcaaggtagttttattccattttgaaccttgaagaacatagttattgtcccctgttaaagggccattatcgtataggcgctgtgaataaactttgaatattgcaatcgtagatagaaaaaacaaaagaataaatcgtttcatatta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF0367:TF0368|TF0367:TF0368:eukaryotic-like metalloproteinase:hypothetical protein:->->:385365..385540 176 atgggacgaactattataaatataagtatttcaaggtagttttattccattttgaaccttgaagaacatagttattgtcccctgttaaagggccattatcgtataggcgctgtgaataaactttgaatattgcaatcgtagatagaaaaaacaaaagaataaatcgtttcatatta Intra-Species Hit: Count: 1 Min: 1 Max: 176 Len: 176 Subject: tfor_TF0367_TF0368|eukaryotic-like metalloproteinase:hypothetical protein|POSITIVE:POSITIVE|[385365,385540]|176 HSP 1 e-value: 2.0E-95 bit: 349.0 Len: 176 Query Start:1 Query End:176 Subject Strand: POSITIVE Subject Start: 1 Subject End: 176 atgggacgaactattataaatataagtatttcaaggtagttttattccattttgaaccttgaagaacatagttattgtcccctgttaaagggccattatcgtataggcgctgtgaataaactttgaatattgcaatcgtagatagaaaaaacaaaagaataaatcgtttcatatta atgggacgaactattataaatataagtatttcaaggtagttttattccattttgaaccttgaagaacatagttattgtcccctgttaaagggccattatcgtataggcgctgtgaataaactttgaatattgcaatcgtagatagaaaaaacaaaagaataaatcgtttcatatta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUGGGACGAACUAUUAUAAAUAUAAGUAUUUCAAGGUAGUUUUAUUCCAUUUUGAACCUUGAAGAACAUAGUUAUUGUCCCCUGUUAAAGGGCCAUUAUCGUAUAGGCGCUGUGAAUAAACUUUGAAUAUUGCAAUCGUAGAUAGAAAAAACAAAAGAAUAAAUCGUUUCAUAUUA (((..((((..((((..........((.(((((((((..................))))))))).)).......((((.((((.....))))...(((((......(((.(((.............))).))).......))))).....))))...))))..))))..))).... (-32.11) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUAUGAAACGAUUUAUUCUUUUGUUUUUUCUAUCUACGAUUGCAAUAUUCAAAGUUUAUUCACAGCGCCUAUACGAUAAUGGCCCUUUAACAGGGGACAAUAACUAUGUUCUUCAAGGUUCAAAAUGGAAUAAAACUACCUUGAAAUACUUAUAUUUAUAAUAGUUCGUCCCAU .....((((..(((((......((((....((.......))..))))......)))))..)))).((.(((...........))).)).......(((((...((((((((..((((((((..................))))))))..))...........)))))).))))).. (-26.37) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 389 117 74 213 173 tfor:TF0723|5end_possible_aspartate_aminotransferase_774234..774464_POSITIVE 373 115 71 212 167 tfor:TF2737|5end_conserved_hypothetical_protein;_TPR-repeat-containing_protein_2918397..2918627_POSITIVE 314 115 74 52 17 tfor:TF0282|5end_probable_tonB-linked_outer_membrane_receptor_309101..309331_POSITIVE 304 113 80 194 166 tfor:TF0479|5end_transcriptional_regulator_497063..497293_POSITIVE 299 111 76 45 6 tfor:TF0396|5end_conserved_hypothetical_protein_417018..417248_POSITIVE 294 116 75 63 9 tfor:TF1237|5end_possible_L-fuculose_kinase_1322041..1322271_POSITIVE 288 110 63 199 147 tfor:TF2379|5end_phosphoribosylformylglycinamidine_cyclo-ligase_(AIR_synthase)_2543849..2544079_POSITIVE 287 134 91 221 163 tfor:TF1529|5end_hypothetical_protein_1634300..1634530_POSITIVE 279 119 76 223 180 tfor:TF1600|5end_uroporphyrinogen-III_synthase_1713964..1714194_POSITIVE 279 115 90 161 141 tfor:TF1037|5end_iron_(III)_ABC_transporter,_ATP-binding_protein_1098194..1098424_POSITIVE 278 138 93 55 18 tfor:TF0690|5end_preprotein_translocase_subunit_735243..735473_POSITIVE 274 114 74 65 27 tfor:TF2654|5end_arginyl-tRNA_synthetase_2822692..2822922_POSITIVE 273 114 76 211 164 tfor:TF0705|5end_hypothetical_protein_750557..750787_POSITIVE 271 110 69 159 107 tfor:TF3156|5end_conserved_hypothetical_protein_3391364..3391594_POSITIVE 270 109 61 151 103 tfor:TF0243|5end_conserved_hypothetical_protein_273728..273958_POSITIVE 268 140 91 63 26 tfor:TF0959|5end_periplasmic_protease_1005218..1005448_POSITIVE 266 139 91 196 132 tfor:TF1252|5end_phosphofructokinase;_pyrophosphate-fructose_6-phosphate_1-phosphotransferase_(probable_beta_subunit)_1334361..1334591_POSITIVE 264 109 61 180 117 tfor:TF0242|5end_conserved_hypothetical_protein_272439..272669_POSITIVE 264 120 73 189 137 tfor:TF0001|5end_hypothetical_protein_-116..114_POSITIVE 261 113 76 88 43 tfor:TF1174|5end_ABC_transporter,_permease_component_1256584..1256814_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 304 113 80 136 108 tfor:TF0477|3end_dipeptide/tripeptide_permease,_POT_family_(proton-dependent_oligopeptide_transport_family)_497085..497235_POSITIVE 293 116 80 48 4 tfor:TF1236|3end_L-rhamnose_isomerase_1322106..1322256_POSITIVE 288 110 63 141 89 tfor:TF2378|3end_peptide_chain_release_factor_1_2543871..2544021_POSITIVE 280 115 79 30 1 tfor:TF0281|3end_hypothetical_protein_309159..309309_POSITIVE 273 93 58 144 113 tfor:TF1517|3end_hypothetical_protein_1626947..1627097_POSITIVE 261 113 76 75 30 tfor:TF1173|3end_ABC_transporter,_permease_component_1256651..1256801_POSITIVE 259 120 80 72 23 tfor:TF2583|3end_possible_ABC_transporter,__permease_component_2751026..2751176_POSITIVE 255 118 78 37 7 tfor:TF1265|3end_dihydroorotate_dehydrogenase_1353414..1353564_POSITIVE 253 140 91 52 9 tfor:TF2082|3end_DNA_topoisomerase_IV,_subunit_B_2250907..2251057_POSITIVE 253 127 81 79 25 tfor:TF1771|3end_prolipoprotein_diacylglyceryl_transferase_1910607..1910757_POSITIVE 251 140 91 67 26 tfor:TF0518|3end_glutaminyl-tRNA_synthetase_543559..543709_POSITIVE 250 139 97 104 63 tfor:TF2310|3end_dehydrogenase,_possible_3-oxoacyl-[acyl-carrier-protein]_reductase_2471283..2471433_POSITIVE 246 120 72 70 28 tfor:TF2822|3end_arginine_repressor_3012379..3012529_POSITIVE 245 125 89 151 120 tfor:TF1282|3end_tRNA_delta-2_isopentenylpyrophosphate_transferase_1367431..1367581_POSITIVE 243 115 75 94 70 tfor:TF2640|3end_hypothetical_protein_2809533..2809683_POSITIVE 243 110 63 43 3 tfor:TF0291|3end_triose-phosphate_isomerase_320131..320281_POSITIVE 242 115 76 146 107 tfor:TF0366|3end_hypothetical_protein_383811..383961_POSITIVE 242 115 76 146 107 tfor:TF0362|3end_hypothetical_protein_380199..380349_POSITIVE 240 80 34 122 80 tfor:TF2795|3end_hypothetical_protein_2980909..2981059_POSITIVE 239 144 108 29 1 tfor:TF2003|3end_dihydrofolate_reductase_2156427..2156577_POSITIVE