Origin IGS: tgatttgcgtctctacggtgcgtactgcgttcatttgcgaaatttgcgttcggacactgtgtaccctgtacataaccattacccgaaagggctgaaagccagatatatttcagccccatcccgtaagggtggggtcccgtccaaccttgttcggaaggctgtaagcctgatgtaatttatcggtcagtattcagccatcagcttccatccaacaatcaaacaactcaacagttcaacaattcaacaactcaacgcccgaagggcatcacttttca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tgaaaagtgatgcccttcgggcgttgagttgttgaattgttgaactgttgagttgtttgattgttggatggaagctgatggctgaatactgaccgataaattacatcaggcttacagccttccgaacaaggttggacgggaccccacccttacgggatggggctgaaatatatctggctttcagccctttcgggtaatggttatgtacagggtacacagtgtccgaacgcaaatttcgcaaatgaacgcagtacgcaccgtagagacgcaaatca Mask Tandem Repeat Region ================================================ tgatttgcgtctctacggtgcgtactgcgttcatttgcgaaatttgcgttcggacactgtgtaccctgtacataaccattacccgaaagggctgaaagccagatatatttcagccccatcccgtaagggtggggtcccgtccaaccttgttcggaaggctgtaagcctgatgtaatttatcggtcagtattcagccatcagcttccatccaacaatcaaacaactcaacagttcaacaattcaacaactcaacgcccgaagggcatcacttttca Find is-nt database================================================ Query_seq: TF0362:TF0364|TF0362:TF0364:hypothetical protein:ysyl endopeptidase:->->:380260..380534 275 tgatttgcgtctctacggtgcgtactgcgttcatttgcgaaatttgcgttcggacactgtgtaccctgtacataaccattacccgaaagggctgaaagccagatatatttcagccccatcccgtaagggtggggtcccgtccaaccttgttcggaaggctgtaagcctgatgtaatttatcggtcagtattcagccatcagcttccatccaacaatcaaacaactcaacagttcaacaattcaacaactcaacgcccgaagggcatcacttttca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF0362:TF0364|TF0362:TF0364:hypothetical protein:ysyl endopeptidase:->->:380260..380534 275 tgatttgcgtctctacggtgcgtactgcgttcatttgcgaaatttgcgttcggacactgtgtaccctgtacataaccattacccgaaagggctgaaagccagatatatttcagccccatcccgtaagggtggggtcccgtccaaccttgttcggaaggctgtaagcctgatgtaatttatcggtcagtattcagccatcagcttccatccaacaatcaaacaactcaacagttcaacaattcaacaactcaacgcccgaagggcatcacttttca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF0362:TF0364|TF0362:TF0364:hypothetical protein:ysyl endopeptidase:->->:380260..380534 275 tgatttgcgtctctacggtgcgtactgcgttcatttgcgaaatttgcgttcggacactgtgtaccctgtacataaccattacccgaaagggctgaaagccagatatatttcagccccatcccgtaagggtggggtcccgtccaaccttgttcggaaggctgtaagcctgatgtaatttatcggtcagtattcagccatcagcttccatccaacaatcaaacaactcaacagttcaacaattcaacaactcaacgcccgaagggcatcacttttca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tgatttgcgtctctacggtgcgtactgcgttcatttgcgaaatttgcgttcggacactgtgtaccctgtacataaccattacccgaaagggctgaaagccagatatatttcagccccatcccgtaagggtggggtcccgtccaaccttgttcggaaggctgtaagcctgatgtaatttatcggtcagtattcagccatcagcttccatccaacaatcaaacaactcaacagttcaacaattcaacaactcaacgcccgaagggcatcacttttca Predict ORF larger than 30AA ================================================ Protein_Len: 30 Strand: + Start: 3 End: 92 .. M C V S T V R T A F I C E I C V R T L C T L Y I T I T R K G ....................................................................................................................................................................................... Protein_Len: 56 Strand: + Start: 5 End: 172 .... M R L Y G A Y C V H L R N L R S D T V Y P V H N H Y P K G L K A R Y I S A P S R K G G V P S N L V R K A V S L M ....................................................................................................... Protein_Len: 33 Strand: + Start: 175 End: 273 .............................................................................................................................................................................. M Y R S V F S H Q L P S N N Q T T Q Q F N N S T T Q R P K G I T F .. Protein_Len: 32 Strand: + Start: 179 End: 274 .................................................................................................................................................................................. M G Q Y S A I S F H P T I K Q L N S S T I Q Q L N A R R A S L F . Protein_Len: 33 Strand: - Start: 82 End: 180 ................................................................................. G F P S F A L Y I E A G D R L P P T G D L R T R F A T L R I Y N M ............................................................................................... Protein_Len: 58 Strand: - Start: 3 End: 176 .. N A D R R H T S R E N A F N A N P C Q T G Q V Y G N G S L A S L W I Y K L G M G Y P H P G T W G Q E S P Q L G S T M ................................................................................................... Protein_Len: 35 Strand: - Start: 2 End: 106 . I Q T E V T R V A N M Q S I Q T R V S H V R Y M V M V R F P Q F G S M ......................................................................................................................................................................... tgatttgcgtctctacggtgcgtactgcgttcatttgcgaaatttgcgttcggacactgtgtaccctgtacataaccattacccgaaagggctgaaagccagatatatttcagccccatcccgtaagggtggggtcccgtccaaccttgttcggaaggctgtaagcctgatgtaatttatcggtcagtattcagccatcagcttccatccaacaatcaaacaactcaacagttcaacaattcaacaactcaacgcccgaagggcatcacttttca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tgatttgcgtctctacggtgcgtactgcgttcatttgcgaaatttgcgttcggacactgtgtaccctgtacataaccattacccgaaagggctgaaagccagatatatttcagccccatcccgtaagggtggggtcccgtccaaccttgttcggaaggctgtaagcctgatgtaatttatcggtcagtattcagccatcagcttccatccaacaatcaaacaactcaacagttcaacaattcaacaactcaacgcccgaagggcatcacttttca Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 63 HP_score: -7.1 Tail_Score: -3.6284 Start: 89 End: 116 Full_Region: cacccttacgggatg gggctgaaa tatatctggc tttcagccc tttcgggtaatggtt ........................................................................................gggctgaaatatatctggctttcagccc............................................................................................................................................................... Find igs database================================================ Query_seq: TF0362:TF0364|TF0362:TF0364:hypothetical protein:ysyl endopeptidase:->->:380260..380534 275 tgatttgcgtctctacggtgcgtactgcgttcatttgcgaaatttgcgttcggacactgtgtaccctgtacataaccattacccgaaagggctgaaagccagatatatttcagccccatcccgtaagggtggggtcccgtccaaccttgttcggaaggctgtaagcctgatgtaatttatcggtcagtattcagccatcagcttccatccaacaatcaaacaactcaacagttcaacaattcaacaactcaacgcccgaagggcatcacttttca Intra-Species Hit: Count: 5 Min: 1 Max: 209 Len: 209 Subject: tfor_TF0362_TF0364|hypothetical protein:ysyl endopeptidase|POSITIVE:POSITIVE|[380260,380534]|275 HSP 1 e-value: 1.0E-115 bit: 414.0 Len: 209 Query Start:1 Query End:209 Subject Strand: POSITIVE Subject Start: 1 Subject End: 209 tgatttgcgtctctacggtgcgtactgcgttcatttgcgaaatttgcgttcggacactgtgtaccctgtacataaccattacccgaaagggctgaaagccagatatatttcagccccatcccgtaagggtggggtcccgtccaaccttgttcggaaggctgtaagcctgatgtaatttatcggtcagtattcagccatcagcttccatc.................................................................. tgatttgcgtctctacggtgcgtactgcgttcatttgcgaaatttgcgttcggacactgtgtaccctgtacataaccattacccgaaagggctgaaagccagatatatttcagccccatcccgtaagggtggggtcccgtccaaccttgttcggaaggctgtaagcctgatgtaatttatcggtcagtattcagccatcagcttccatc.................................................................. Subject: tfor_TF2166_TF2167|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[2342423,2342725]|303 HSP 1 e-value: 1.0E-112 bit: 406.0 Len: 209 Query Start:1 Query End:209 Subject Strand: POSITIVE Subject Start: 1 Subject End: 209 tgatttgcgtctctacggtgcgtactgcgttcatttgcgaaatttgcgttcggacactgtgtaccctgtacataaccattacccgaaagggctgaaagccagatatatttcagccccatcccgtaagggtggggtcccgtccaaccttgttcggaaggctgtaagcctgatgtaatttatcggtcagtattcagccatcagcttccatc.................................................................. tgatttgcgtctctacggtgcgtactgcgttcatttgcgaaatttgcgttcggacaccgtgtaccctgtacataaccattacccgaaagggctgaaagccagatatatttcagccccatcccgtaagggtggggtcccgtccaaccttgttcggaaggctgtaagcctgatgtaatttatcggtcagtattcagccatcagcttccatc.................................................................. Subject: tfor_TF0366_TF0367|hypothetical protein:eukaryotic-like metalloproteinase|POSITIVE:POSITIVE|[383872,384146]|275 HSP 1 e-value: 1.0E-112 bit: 406.0 Len: 209 Query Start:1 Query End:209 Subject Strand: POSITIVE Subject Start: 1 Subject End: 209 tgatttgcgtctctacggtgcgtactgcgttcatttgcgaaatttgcgttcggacactgtgtaccctgtacataaccattacccgaaagggctgaaagccagatatatttcagccccatcccgtaagggtggggtcccgtccaaccttgttcggaaggctgtaagcctgatgtaatttatcggtcagtattcagccatcagcttccatc.................................................................. tgatttgcgtctctacggtgcgtactgcgttcatttgcgaaatttgcgttcggacaccgtgtaccctgtacataaccattacccgaaagggctgaaagccagatatatttcagccccatcccgtaagggtggggtcccgtccaaccttgttcggaaggctgtaagcctgatgtaatttatcggtcagtattcagccatcagcttccatc.................................................................. Subject: tfor_TF0346_TF0347|hypothetical protein:possible serine protease|POSITIVE:POSITIVE|[367377,367681]|305 HSP 1 e-value: 3.0E-98 bit: 359.0 Len: 205 Query Start:5 Query End:209 Subject Strand: POSITIVE Subject Start: 5 Subject End: 209 ....ttgcgtctctacggtgcgtactgcgttcatttgcgaaatttgcgttcggacactgtgtaccctgtacataaccattacccgaaagggctgaaagccagatatatttcagccccatcccgtaagggtggggtcccgtccaaccttgttcggaaggctgtaagcctgatgtaatttatcggtcagtattcagccatcagcttccatc.................................................................. ....ttgcgtctctacggtgcgtactgcgttcatttgcgaaatttgcattcggacaccgtgtaccctgtacataaccattacccgaaagggctgcaaacccgatatatttcagccccatcccgtaagggtggggtcccgtccaaccttgttcggagggctgtaagcctgatgtaatttatcggtcagtattcagccatcagcttccatc.................................................................. Subject: tfor_TF2161_TF2162|conserved hypothetical protein:thermolysin precursor|POSITIVE:POSITIVE|[2338423,2338808]|386 HSP 1 e-value: 1.0E-42 bit: 174.0 Len: 116 Query Start:87 Query End:202 Subject Strand: POSITIVE Subject Start: 168 Subject End: 283 ......................................................................................aagggctgaaagccagatatatttcagccccatcccgtaagggtggggtcccgtccaaccttgttcggaaggctgtaagcctgatgtaatttatcggtcagtattcagccatcagc......................................................................... ......................................................................................aagggctgaaagcccgttctgtttcagccccatcccgtaagggtggggtcccgtcccaccttgttcggagggctgtaagcctgatgtaatttatcggtcagtattcagcaatcagc......................................................................... Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGAUUUGCGUCUCUACGGUGCGUACUGCGUUCAUUUGCGAAAUUUGCGUUCGGACACUGUGUACCCUGUACAUAACCAUUACCCGAAAGGGCUGAAAGCCAGAUAUAUUUCAGCCCCAUCCCGUAAGGGUGGGGUCCCGUCCAACCUUGUUCGGAAGGCUGUAAGCCUGAUGUAAUUUAUCGGUCAGUAUUCAGCCAUCAGCUUCCAUC .....(((((..(....).)))))....((((...(((((...)))))...))))..(((((((...))))))).......(((....))).((.((((.............((((((.(((....))))))))).(((..((....))..)))..(((((...((((((((.....))))))...))...)))))....)))).)).. (-63.10) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GAUGGAAGCUGAUGGCUGAAUACUGACCGAUAAAUUACAUCAGGCUUACAGCCUUCCGAACAAGGUUGGACGGGACCCCACCCUUACGGGAUGGGGCUGAAAUAUAUCUGGCUUUCAGCCCUUUCGGGUAAUGGUUAUGUACAGGGUACACAGUGUCCGAACGCAAAUUUCGCAAAUGAACGCAGUACGCACCGUAGAGACGCAAAUCA (((.....((((.(((((((....(.((((((.(((...((((.....(((((((......))))))).......((((((((....))).)))))))))))).)))).))).)))))))...))))...(((((..(((((.((..((...))..)).............((........)).))))).)))))..........))). (-58.40) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 839 130 83 213 166 tfor:TF0822|5end_hypothetical_protein_880487..880717_POSITIVE 839 130 83 213 166 tfor:TF0821|5end_hypothetical_protein_879879..880109_POSITIVE 779 140 91 55 6 tfor:TF2840|5end_hypothetical_protein_3031060..3031290_POSITIVE 763 130 83 199 152 tfor:TF0678|5end_hypothetical_protein_721103..721333_POSITIVE 757 160 111 84 29 tfor:TF2547|5end_hypothetical_protein_2730664..2730894_POSITIVE 664 100 51 169 122 tfor:TF3105|5end_hypothetical_protein_3338068..3338298_POSITIVE 647 117 71 231 178 tfor:TF2191|5end_hypothetical_protein_2365934..2366164_POSITIVE 574 70 26 77 24 tfor:TF1183|5end_hypothetical_protein_1268391..1268621_POSITIVE 569 189 143 231 181 tfor:TF1655|5end_long-chain-fatty-acid-CoA_ligase_1769279..1769509_POSITIVE 555 100 51 221 171 tfor:TF0679|5end_hypothetical_protein_721155..721385_POSITIVE 546 59 16 99 45 tfor:TF1184|5end_hypothetical_protein_1269004..1269234_POSITIVE 505 170 122 187 137 tfor:TF0499|5end_hypothetical_protein_526257..526487_POSITIVE 498 70 22 158 115 tfor:TF2816|5end_hypothetical_protein_3006925..3007155_POSITIVE 476 57 17 64 16 tfor:TF2652|5end_hypothetical_protein_2821803..2822033_POSITIVE 470 170 122 226 175 tfor:TF2745|5end_hypothetical_protein_2927562..2927792_POSITIVE 470 175 131 165 119 tfor:TF1274|5end_hypothetical_protein_1357621..1357851_POSITIVE 465 59 16 133 79 tfor:TF1187|5end_hypothetical_protein_1269625..1269855_POSITIVE 465 59 16 76 22 tfor:TF1186|5end_hypothetical_protein_1269568..1269798_POSITIVE 464 175 131 65 19 tfor:TF3003|5end_hypothetical_protein_3220382..3220612_POSITIVE 461 80 32 114 74 tfor:TF1660|5end_hypothetical_protein_1775269..1775499_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 560 179 131 89 44 tfor:TF1762|3end_hypothetical_protein_1899648..1899798_POSITIVE 504 179 131 62 19 tfor:TF0018|3end_hypothetical_protein_12091..12241_POSITIVE 473 170 122 71 25 tfor:TF2922|3end_surface_antigen_BspA_3121538..3121688_POSITIVE 462 175 131 86 42 tfor:TF0344|3end_hypothetical_protein_366963..367113_POSITIVE 462 175 131 46 2 tfor:TF0343|3end_hypothetical_protein_366923..367073_POSITIVE 460 53 16 129 85 tfor:TF1841|3end_hypothetical_protein_1992267..1992417_POSITIVE 452 59 13 64 10 tfor:TF2823|3end_TPR-containing_protein_3014337..3014487_POSITIVE 444 173 131 77 33 tfor:TF2350|3end_hypothetical_protein_2515364..2515514_POSITIVE 442 180 133 53 1 tfor:TF0677|3end_ATP-binding_protein,_Mrp/Nbp35_family,_involved_in_chromosome_partitioning_720982..721132_POSITIVE 425 198 152 54 5 tfor:TF2047|3end_hypothetical_protein_2210298..2210448_POSITIVE 421 160 111 150 97 tfor:TF1274|3end_hypothetical_protein_1357895..1358045_POSITIVE 416 70 21 42 1 tfor:TF2816|3end_hypothetical_protein_3007184..3007334_POSITIVE 414 175 136 53 20 tfor:TF2169|3end_hypothetical_protein_2345354..2345504_POSITIVE 414 175 136 42 9 tfor:TF0361|3end_hypothetical_protein_379810..379960_POSITIVE 402 160 111 51 2 tfor:TF2172|3end_hypothetical_protein_2346135..2346285_POSITIVE 402 169 125 144 106 tfor:TF1138|3end_ribosomal_large_subunit_pseudouridine_synthase_B_1215238..1215388_POSITIVE 393 169 121 54 1 tfor:TF1743|3end_conserved_hypothetical_protein_1877909..1878059_POSITIVE 378 158 121 145 102 tfor:TF2281|3end_hypothetical_protein_2439995..2440145_POSITIVE 378 66 32 34 1 tfor:TF1838|3end_tRNA/rRNA_methyltransferase_1988745..1988895_POSITIVE 377 200 151 80 36 tfor:TF2251|3end_conjugative_transposon_protein,_TraE_2424687..2424837_POSITIVE