Origin IGS: gcaagtttgtttaagtaaaacatctcgaccattaaataaatcaacattttatatcagacttacagccctccgcaggagtgggtattcttttcccccaacgctttgcttcgctacacattgggctgaattaacgcaccccttcggagctgttaca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tgtaacagctccgaaggggtgcgttaattcagcccaatgtgtagcgaagcaaagcgttgggggaaaagaatacccactcctgcggagggctgtaagtctgatataaaatgttgatttatttaatggtcgagatgttttacttaaacaaacttgc Mask Tandem Repeat Region ================================================ gcaagtttgtttaagtaaaacatctcgaccattaaataaatcaacattttatatcagacttacagccctccgcaggagtgggtattcttttcccccaacgctttgcttcgctacacattgggctgaattaacgcaccccttcggagctgttaca Find is-nt database================================================ Query_seq: TF0349:TF0350|TF0349:TF0350:hypothetical protein:hypothetical protein:->->:370780..370933 154 gcaagtttgtttaagtaaaacatctcgaccattaaataaatcaacattttatatcagacttacagccctccgcaggagtgggtattcttttcccccaacgctttgcttcgctacacattgggctgaattaacgcaccccttcggagctgttaca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF0349:TF0350|TF0349:TF0350:hypothetical protein:hypothetical protein:->->:370780..370933 154 gcaagtttgtttaagtaaaacatctcgaccattaaataaatcaacattttatatcagacttacagccctccgcaggagtgggtattcttttcccccaacgctttgcttcgctacacattgggctgaattaacgcaccccttcggagctgttaca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF0349:TF0350|TF0349:TF0350:hypothetical protein:hypothetical protein:->->:370780..370933 154 gcaagtttgtttaagtaaaacatctcgaccattaaataaatcaacattttatatcagacttacagccctccgcaggagtgggtattcttttcccccaacgctttgcttcgctacacattgggctgaattaacgcaccccttcggagctgttaca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 gcaagtttgtttaagtaaaacatctcgaccattaaataaatcaacattttatatcagacttacagccctccgcaggagtgggtattcttttcccccaacgctttgcttcgctacacattgggctgaattaacgcaccccttcggagctgttaca Predict ORF larger than 30AA ================================================ Protein_Len: 31 Strand: + Start: 36 End: 128 ................................... M N Q H F I S D L Q P S A G V G I L F P Q R F A S L H I G L N .......................... Protein_Len: 34 Strand: + Start: 53 End: 154 .................................................... M R L T A L R R S G Y S F P P T L C F A T H W A E L T H P F G A V T gcaagtttgtttaagtaaaacatctcgaccattaaataaatcaacattttatatcagacttacagccctccgcaggagtgggtattcttttcccccaacgctttgcttcgctacacattgggctgaattaacgcaccccttcggagctgttaca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ gcaagtttgtttaagtaaaacatctcgaccattaaataaatcaacattttatatcagacttacagccctccgcaggagtgggtattcttttcccccaacgctttgcttcgctacacattgggctgaattaacgcaccccttcggagctgttaca Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF0349:TF0350|TF0349:TF0350:hypothetical protein:hypothetical protein:->->:370780..370933 154 gcaagtttgtttaagtaaaacatctcgaccattaaataaatcaacattttatatcagacttacagccctccgcaggagtgggtattcttttcccccaacgctttgcttcgctacacattgggctgaattaacgcaccccttcggagctgttaca Intra-Species Hit: Count: 7 Min: 1 Max: 154 Len: 154 Subject: tfor_TF0349_TF0350|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[370780,370933]|154 HSP 1 e-value: 2.0E-82 bit: 305.0 Len: 154 Query Start:1 Query End:154 Subject Strand: POSITIVE Subject Start: 1 Subject End: 154 gcaagtttgtttaagtaaaacatctcgaccattaaataaatcaacattttatatcagacttacagccctccgcaggagtgggtattcttttcccccaacgctttgcttcgctacacattgggctgaattaacgcaccccttcggagctgttaca gcaagtttgtttaagtaaaacatctcgaccattaaataaatcaacattttatatcagacttacagccctccgcaggagtgggtattcttttcccccaacgctttgcttcgctacacattgggctgaattaacgcaccccttcggagctgttaca Subject: tfor_TF0365_TF0366|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[383213,383703]|491 HSP 1 e-value: 5.0E-80 bit: 297.0 Len: 154 Query Start:1 Query End:154 Subject Strand: POSITIVE Subject Start: 271 Subject End: 424 gcaagtttgtttaagtaaaacatctcgaccattaaataaatcaacattttatatcagacttacagccctccgcaggagtgggtattcttttcccccaacgctttgcttcgctacacattgggctgaattaacgcaccccttcggagctgttaca gcaagtttgtttaagtaaaacatctcgaccattaaataaatcaacattttatatcaggcttacagccctccgcaggagtgggtattcttttcccccaacgctttgcttcgctacacattgggctgaattaacgcaccccttcggagctgttaca Subject: tfor_TF0361_TF0362|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[379871,380091]|221 HSP 1 e-value: 1.0E-77 bit: 289.0 Len: 154 Query Start:1 Query End:154 Subject Strand: POSITIVE Subject Start: 1 Subject End: 154 gcaagtttgtttaagtaaaacatctcgaccattaaataaatcaacattttatatcagacttacagccctccgcaggagtgggtattcttttcccccaacgctttgcttcgctacacattgggctgaattaacgcaccccttcggagctgttaca gcaagtttgtttaagtaaaacatctcgaccattaaataaatcaacattttatatcaggcttacagccctccgcaggagtgggtattcttttcccccaacgctttgcttcgctacacattgggctgaattaacgcatcccttcggagctgttaca Subject: tfor_TF0358_TF0359|hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[376365,377082]|718 HSP 1 e-value: 3.0E-75 bit: 281.0 Len: 154 Query Start:1 Query End:154 Subject Strand: POSITIVE Subject Start: 266 Subject End: 419 gcaagtttgtttaagtaaaacatctcgaccattaaataaatcaacattttatatcagacttacagccctccgcaggagtgggtattcttttcccccaacgctttgcttcgctacacattgggctgaattaacgcaccccttcggagctgttaca gcaagtttgtttaagtaaaacatctcgaccattaaataaatcaacattttatatcaggctttcagccctccgcaggagtgggtattcttttcccccaacgcttcgcttcgctacacattgggctgaattaacgcaccccttcggagctgttaca Subject: tfor_TF2108_TF2109|hypothetical protein:possible metallo-beta-lactamase family protein|POSITIVE:POSITIVE|[2277750,2278081]|332 HSP 1 e-value: 4.0E-59 bit: 228.0 Len: 150 Query Start:5 Query End:154 Subject Strand: POSITIVE Subject Start: 1 Subject End: 151 ....gtttgtttaagtaaaacatctcgaccattaaataaatcaacattttatatcagacttacagccctccgcaggagtgggtattc-ttttcccccaacgctttgcttcgctacacattgggctgaattaacgcaccccttcggagctgttaca ....gtttgtttaagtaaaacatctcgaccattaaataaattaatattttatataaggtttacagccctccgcaggagtgggtattcttttttccccaacgcttcgcttcgctacacattgggctgaattaacgcaccccttcggagctgttaca Subject: tfor_TF2169_TF2171|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[2345415,2345624]|210 HSP 1 e-value: 1.0E-56 bit: 220.0 Len: 143 Query Start:12 Query End:154 Subject Strand: POSITIVE Subject Start: 1 Subject End: 143 ...........taagtaaaacatctcgaccattaaataaatcaacattttatatcagacttacagccctccgcaggagtgggtattcttttcccccaacgctttgcttcgctacacattgggctgaattaacgcaccccttcggagctgttaca ...........taagtaaaacatctcgaccattaaataaatcaacattttatatcaggcttacagccctccgcaggagtgggtattcttttttcccaatgcttcgcttcgctacacattgggctgaattaatgcgcccctgcggagctgttaca Subject: tfor_TF2165_TF2166|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[2341983,2342254]|272 HSP 1 e-value: 3.0E-29 bit: 129.0 Len: 69 Query Start:1 Query End:69 Subject Strand: POSITIVE Subject Start: 62 Subject End: 130 gcaagtttgtttaagtaaaacatctcgaccattaaataaatcaacattttatatcagacttacagccct..................................................................................... gcaagtttgtttaagtaaaacatctcgaccattaaataaatcaacattttatatcaggcttacagccct..................................................................................... HSP 2 e-value: 1.0E-6 bit: 54.0 Len: 39 Query Start:116 Query End:154 Subject Strand: POSITIVE Subject Start: 167 Subject End: 205 ...................................................................................................................cattgggctgaattaacgcaccccttcggagctgttaca ...................................................................................................................cattgggctgaattaatgcgcccctgcggagctgttaca Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GCAAGUUUGUUUAAGUAAAACAUCUCGACCAUUAAAUAAAUCAACAUUUUAUAUCAGACUUACAGCCCUCCGCAGGAGUGGGUAUUCUUUUCCCCCAACGCUUUGCUUCGCUACACAUUGGGCUGAAUUAACGCACCCCUUCGGAGCUGUUACA ....((((((((((.................))))))))))....................(((((..((((.(((.(((.((((((......(((((.(....((...))....).)))))..))))..)).))).))).))))))))).... (-31.73) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGUAACAGCUCCGAAGGGGUGCGUUAAUUCAGCCCAAUGUGUAGCGAAGCAAAGCGUUGGGGGAAAAGAAUACCCACUCCUGCGGAGGGCUGUAAGUCUGAUAUAAAAUGUUGAUUUAUUUAAUGGUCGAGAUGUUUUACUUAAACAAACUUGC ....(((((((((.(((((((.((...(((..((((((((...((...))...)))))))).)))......)).))))))).))))..)))))..(((((((...(((((......)))))....))).))))((((.....))))........ (-43.20) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 882 140 93 103 56 tfor:TF1716|5end_hypothetical_protein_1837442..1837672_POSITIVE 717 140 93 114 68 tfor:TF0538|5end_hypothetical_protein_566891..567121_POSITIVE 717 140 93 107 61 tfor:TF0539|5end_hypothetical_protein_566884..567114_POSITIVE 702 140 92 202 146 tfor:TF1406|5end_transposase_1481758..1481988_POSITIVE 651 130 81 98 47 tfor:TF0679|5end_hypothetical_protein_721155..721385_POSITIVE 617 140 93 55 8 tfor:TF0678|5end_hypothetical_protein_721103..721333_POSITIVE 587 110 63 186 141 tfor:TF2478|5end_hypothetical_protein_2655375..2655605_POSITIVE 585 106 61 84 40 tfor:TF2191|5end_hypothetical_protein_2365934..2366164_POSITIVE 585 106 61 219 175 tfor:TF0823|5end_conserved_hypothetical_protein_880660..880890_POSITIVE 585 106 61 46 2 tfor:TF0822|5end_hypothetical_protein_880487..880717_POSITIVE 569 148 101 176 130 tfor:TF2517|5end_hypothetical_protein_2695089..2695319_POSITIVE 568 109 61 68 20 tfor:TF3107|5end_hypothetical_protein_3338459..3338689_POSITIVE 568 140 93 181 144 tfor:TF2824|5end_nucleoside-transporting_protein_nupG_3014701..3014931_POSITIVE 558 106 63 167 125 tfor:TF0828|5end_hypothetical_protein_887090..887320_POSITIVE 546 106 61 46 2 tfor:TF0821|5end_hypothetical_protein_879879..880109_POSITIVE 514 106 61 218 173 tfor:TF1187|5end_hypothetical_protein_1269625..1269855_POSITIVE 514 106 61 161 116 tfor:TF1186|5end_hypothetical_protein_1269568..1269798_POSITIVE 503 106 62 157 113 tfor:TF1183|5end_hypothetical_protein_1268391..1268621_POSITIVE 499 140 99 114 74 tfor:TF0534|5end_hypothetical_protein_559509..559739_POSITIVE 439 150 101 218 177 tfor:TF1184|5end_hypothetical_protein_1269004..1269234_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 677 138 94 72 28 tfor:TF2840|3end_hypothetical_protein_3031289..3031439_POSITIVE 568 109 61 137 89 tfor:TF3106|3end_hypothetical_protein_3338608..3338758_POSITIVE 559 110 63 145 97 tfor:TF1405|3end_conserved_hypothetical_protein_1481819..1481969_POSITIVE 544 100 54 108 62 tfor:TF2547|3end_hypothetical_protein_2730947..2731097_POSITIVE 514 106 61 76 31 tfor:TF1185|3end_hypothetical_protein_1269563..1269713_POSITIVE 499 140 99 44 4 tfor:TF0533|3end_hypothetical_protein_559519..559669_POSITIVE 439 150 101 72 31 tfor:TF1182|3end_hypothetical_protein_1268938..1269088_POSITIVE 439 150 101 63 22 tfor:TF0820|3end_hypothetical_protein_879942..880092_POSITIVE 434 106 63 139 97 tfor:TF1274|3end_hypothetical_protein_1357895..1358045_POSITIVE 430 112 75 40 4 tfor:TF0370|3end_hypothetical_protein_386317..386467_POSITIVE 390 107 65 61 19 tfor:TF0821|3end_hypothetical_protein_880195..880345_POSITIVE 359 106 62 62 22 tfor:TF2342|3end_glutathione_peroxidase_2506639..2506789_POSITIVE 351 96 54 128 80 tfor:TF2816|3end_hypothetical_protein_3007184..3007334_POSITIVE 347 109 63 70 10 tfor:TF0289|3end_conserved_hypothetical_protein_319363..319513_POSITIVE 342 109 64 72 23 tfor:TF2650|3end_succinate_dehydrogenase,_iron-sulfur_subunit_2821806..2821956_POSITIVE 340 106 63 87 44 tfor:TF0340|3end_hypothetical_protein_364257..364407_POSITIVE 336 140 93 62 7 tfor:TF1474|3end_6-phosphogluconolactonase_1568123..1568273_POSITIVE 336 100 56 113 69 tfor:TF1002|3end_replicative_DNA_helicase_1058232..1058382_POSITIVE 335 107 66 53 20 tfor:TF2651|3end_hypothetical_protein_2822014..2822164_POSITIVE 332 85 54 151 122 tfor:TF0678|3end_hypothetical_protein_721335..721485_POSITIVE