Origin IGS: atcattgcgtctctacggtgcgtactgcgttcatttgcgaaatttgcattcggacaccgtgtaccctgtacataaccattacccgaaagggctgcaaacccgatatatttcagccccatcccgtaagggtggggtcccgtccaaccttgttcggagggctgtaagcctgatgtaatttatcggtcagtattcagccatcagcttccatccaacaatcaaacaactcaacagttcaacaattcaacaattcaacgcccgaagggcatcacttttcacttttcgtttctcacttttcacttttctca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tgagaaaagtgaaaagtgagaaacgaaaagtgaaaagtgatgcccttcgggcgttgaattgttgaattgttgaactgttgagttgtttgattgttggatggaagctgatggctgaatactgaccgataaattacatcaggcttacagccctccgaacaaggttggacgggaccccacccttacgggatggggctgaaatatatcgggtttgcagccctttcgggtaatggttatgtacagggtacacggtgtccgaatgcaaatttcgcaaatgaacgcagtacgcaccgtagagacgcaatgat Mask Tandem Repeat Region ================================================ atcattgcgtctctacggtgcgtactgcgttcatttgcgaaatttgcattcggacaccgtgtaccctgtacataaccattacccgaaagggctgcaaacccgatatatttcagccccatcccgtaagggtggggtcccgtccaaccttgttcggagggctgtaagcctgatgtaatttatcggtcagtattcagccatcagcttccatcnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnngcccgaagggcatcacttttcacttttcgtttctcacttttcacttttctca Find is-nt database================================================ Query_seq: TF0346:TF0347|TF0346:TF0347:hypothetical protein:possible serine protease:->->:367377..367681 305 atcattgcgtctctacggtgcgtactgcgttcatttgcgaaatttgcattcggacaccgtgtaccctgtacataaccattacccgaaagggctgcaaacccgatatatttcagccccatcccgtaagggtggggtcccgtccaaccttgttcggagggctgtaagcctgatgtaatttatcggtcagtattcagccatcagcttccatccaacaatcaaacaactcaacagttcaacaattcaacaattcaacgcccgaagggcatcacttttcacttttcgtttctcacttttcacttttctca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF0346:TF0347|TF0346:TF0347:hypothetical protein:possible serine protease:->->:367377..367681 305 atcattgcgtctctacggtgcgtactgcgttcatttgcgaaatttgcattcggacaccgtgtaccctgtacataaccattacccgaaagggctgcaaacccgatatatttcagccccatcccgtaagggtggggtcccgtccaaccttgttcggagggctgtaagcctgatgtaatttatcggtcagtattcagccatcagcttccatccaacaatcaaacaactcaacagttcaacaattcaacaattcaacgcccgaagggcatcacttttcacttttcgtttctcacttttcacttttctca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF0346:TF0347|TF0346:TF0347:hypothetical protein:possible serine protease:->->:367377..367681 305 atcattgcgtctctacggtgcgtactgcgttcatttgcgaaatttgcattcggacaccgtgtaccctgtacataaccattacccgaaagggctgcaaacccgatatatttcagccccatcccgtaagggtggggtcccgtccaaccttgttcggagggctgtaagcctgatgtaatttatcggtcagtattcagccatcagcttccatccaacaatcaaacaactcaacagttcaacaattcaacaattcaacgcccgaagggcatcacttttcacttttcgtttctcacttttcacttttctca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 atcattgcgtctctacggtgcgtactgcgttcatttgcgaaatttgcattcggacaccgtgtaccctgtacataaccattacccgaaagggctgcaaacccgatatatttcagccccatcccgtaagggtggggtcccgtccaaccttgttcggagggctgtaagcctgatgtaatttatcggtcagtattcagccatcagcttccatccaacaatcaaacaactcaacagttcaacaattcaacaattcaacgcccgaagggcatcacttttcacttttcgtttctcacttttcacttttctca Predict ORF larger than 30AA ================================================ Protein_Len: 48 Strand: + Start: 18 End: 161 ................. M R T A F I C E I C I R T P C T L Y I T I T R K G C K P D I F Q P H P V R V G S R P T L F G G L ................................................................................................................................................ Protein_Len: 56 Strand: + Start: 5 End: 172 .... M R L Y G A Y C V H L R N L H S D T V Y P V H N H Y P K G L Q T R Y I S A P S R K G G V P S N L V R R A V S L M ..................................................................................................................................... Protein_Len: 43 Strand: + Start: 175 End: 303 .............................................................................................................................................................................. M Y R S V F S H Q L P S N N Q T T Q Q F N N S T I Q R P K G I T F H F S F L T F H F S .. Protein_Len: 42 Strand: + Start: 179 End: 304 .................................................................................................................................................................................. M G Q Y S A I S F H P T I K Q L N S S T I Q Q F N A R R A S L F T F R F S L F T F L . Protein_Len: 39 Strand: + Start: 189 End: 305 ............................................................................................................................................................................................ M Q P S A S I Q Q S N N S T V Q Q F N N S T P E G H H F S L F V S H F S L F S Protein_Len: 33 Strand: - Start: 82 End: 180 ................................................................................. G F P S C V R Y I E A G D R L P P T G D L R T R L A T L R I Y N M ............................................................................................................................. Protein_Len: 58 Strand: - Start: 3 End: 176 .. M A D R R H T S R E N A F N A N P C R T G Q V Y G N G S L A A F G I Y K L G M G Y P H P G T W G Q E S P Q L G S T M ................................................................................................................................. Protein_Len: 35 Strand: - Start: 5 End: 106 .... * Q T E V T R V A N M Q S I Q M R V G H V R Y M V M V R F P Q L G S M ....................................................................................................................................................................................................... atcattgcgtctctacggtgcgtactgcgttcatttgcgaaatttgcattcggacaccgtgtaccctgtacataaccattacccgaaagggctgcaaacccgatatatttcagccccatcccgtaagggtggggtcccgtccaaccttgttcggagggctgtaagcctgatgtaatttatcggtcagtattcagccatcagcttccatccaacaatcaaacaactcaacagttcaacaattcaacaattcaacgcccgaagggcatcacttttcacttttcgtttctcacttttcacttttctca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ atcattgcgtctctacggtgcgtactgcgttcatttgcgaaatttgcattcggacaccgtgtaccctgtacataaccattacccgaaagggctgcaaacccgatatatttcagccccatcccgtaagggtggggtcccgtccaaccttgttcggagggctgtaagcctgatgtaatttatcggtcagtattcagccatcagcttccatccaacaatcaaacaactcaacagttcaacaattcaacaattcaacgcccgaagggcatcacttttcacttttcgtttctcacttttcacttttctca Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF0346:TF0347|TF0346:TF0347:hypothetical protein:possible serine protease:->->:367377..367681 305 atcattgcgtctctacggtgcgtactgcgttcatttgcgaaatttgcattcggacaccgtgtaccctgtacataaccattacccgaaagggctgcaaacccgatatatttcagccccatcccgtaagggtggggtcccgtccaaccttgttcggagggctgtaagcctgatgtaatttatcggtcagtattcagccatcagcttccatccaacaatcaaacaactcaacagttcaacaattcaacaattcaacgcccgaagggcatcacttttcacttttcgtttctcacttttcacttttctca Intra-Species Hit: Count: 6 Min: 1 Max: 209 Len: 209 Subject: tfor_TF0346_TF0347|hypothetical protein:possible serine protease|POSITIVE:POSITIVE|[367377,367681]|305 HSP 1 e-value: 1.0E-115 bit: 414.0 Len: 209 Query Start:1 Query End:209 Subject Strand: POSITIVE Subject Start: 1 Subject End: 209 atcattgcgtctctacggtgcgtactgcgttcatttgcgaaatttgcattcggacaccgtgtaccctgtacataaccattacccgaaagggctgcaaacccgatatatttcagccccatcccgtaagggtggggtcccgtccaaccttgttcggagggctgtaagcctgatgtaatttatcggtcagtattcagccatcagcttccatc................................................................................................ atcattgcgtctctacggtgcgtactgcgttcatttgcgaaatttgcattcggacaccgtgtaccctgtacataaccattacccgaaagggctgcaaacccgatatatttcagccccatcccgtaagggtggggtcccgtccaaccttgttcggagggctgtaagcctgatgtaatttatcggtcagtattcagccatcagcttccatc................................................................................................ Subject: tfor_TF2166_TF2167|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[2342423,2342725]|303 HSP 1 e-value: 1.0E-100 bit: 367.0 Len: 205 Query Start:5 Query End:209 Subject Strand: POSITIVE Subject Start: 5 Subject End: 209 ....ttgcgtctctacggtgcgtactgcgttcatttgcgaaatttgcattcggacaccgtgtaccctgtacataaccattacccgaaagggctgcaaacccgatatatttcagccccatcccgtaagggtggggtcccgtccaaccttgttcggagggctgtaagcctgatgtaatttatcggtcagtattcagccatcagcttccatc................................................................................................ ....ttgcgtctctacggtgcgtactgcgttcatttgcgaaatttgcgttcggacaccgtgtaccctgtacataaccattacccgaaagggctgaaagccagatatatttcagccccatcccgtaagggtggggtcccgtccaaccttgttcggaaggctgtaagcctgatgtaatttatcggtcagtattcagccatcagcttccatc................................................................................................ Subject: tfor_TF0366_TF0367|hypothetical protein:eukaryotic-like metalloproteinase|POSITIVE:POSITIVE|[383872,384146]|275 HSP 1 e-value: 1.0E-100 bit: 367.0 Len: 205 Query Start:5 Query End:209 Subject Strand: POSITIVE Subject Start: 5 Subject End: 209 ....ttgcgtctctacggtgcgtactgcgttcatttgcgaaatttgcattcggacaccgtgtaccctgtacataaccattacccgaaagggctgcaaacccgatatatttcagccccatcccgtaagggtggggtcccgtccaaccttgttcggagggctgtaagcctgatgtaatttatcggtcagtattcagccatcagcttccatc................................................................................................ ....ttgcgtctctacggtgcgtactgcgttcatttgcgaaatttgcgttcggacaccgtgtaccctgtacataaccattacccgaaagggctgaaagccagatatatttcagccccatcccgtaagggtggggtcccgtccaaccttgttcggaaggctgtaagcctgatgtaatttatcggtcagtattcagccatcagcttccatc................................................................................................ Subject: tfor_TF0362_TF0364|hypothetical protein:ysyl endopeptidase|POSITIVE:POSITIVE|[380260,380534]|275 HSP 1 e-value: 4.0E-98 bit: 359.0 Len: 205 Query Start:5 Query End:209 Subject Strand: POSITIVE Subject Start: 5 Subject End: 209 ....ttgcgtctctacggtgcgtactgcgttcatttgcgaaatttgcattcggacaccgtgtaccctgtacataaccattacccgaaagggctgcaaacccgatatatttcagccccatcccgtaagggtggggtcccgtccaaccttgttcggagggctgtaagcctgatgtaatttatcggtcagtattcagccatcagcttccatc................................................................................................ ....ttgcgtctctacggtgcgtactgcgttcatttgcgaaatttgcgttcggacactgtgtaccctgtacataaccattacccgaaagggctgaaagccagatatatttcagccccatcccgtaagggtggggtcccgtccaaccttgttcggaaggctgtaagcctgatgtaatttatcggtcagtattcagccatcagcttccatc................................................................................................ Subject: tfor_TF2161_TF2162|conserved hypothetical protein:thermolysin precursor|POSITIVE:POSITIVE|[2338423,2338808]|386 HSP 1 e-value: 1.0E-42 bit: 174.0 Len: 116 Query Start:87 Query End:202 Subject Strand: POSITIVE Subject Start: 168 Subject End: 283 ......................................................................................aagggctgcaaacccgatatatttcagccccatcccgtaagggtggggtcccgtccaaccttgttcggagggctgtaagcctgatgtaatttatcggtcagtattcagccatcagc....................................................................................................... ......................................................................................aagggctgaaagcccgttctgtttcagccccatcccgtaagggtggggtcccgtcccaccttgttcggagggctgtaagcctgatgtaatttatcggtcagtattcagcaatcagc....................................................................................................... Subject: tfor_TF0029_TF0030|hypothetical protein:N-acetylneuraminate lyase|POSITIVE:POSITIVE|[25123,25909]|787 HSP 1 e-value: 8.0E-10 bit: 65.9 Len: 109 Query Start:87 Query End:195 Subject Strand: NEGATIVE Subject Start: 146 Subject End: 260 ......................................................................................aagggctgcaaacccgatatatttcagccccatcccgtaagggtgggg--tcc-cgtcca-accttgttcggagggctgtaagcctgatgtaat--ttatcggtcagtattcagc.............................................................................................................. ......................................................................................aagggctggaagcccgaattatttcagccccatcccgtaagggtgggggatccatgcccataccttggttagagggctgtaagcctgatataatcgttagcggtcagtattcagc.............................................................................................................. Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUCAUUGCGUCUCUACGGUGCGUACUGCGUUCAUUUGCGAAAUUUGCAUUCGGACACCGUGUACCCUGUACAUAACCAUUACCCGAAAGGGCUGCAAACCCGAUAUAUUUCAGCCCCAUCCCGUAAGGGUGGGGUCCCGUCCAACCUUGUUCGGAGGGCUGUAAGCCUGAUGUAAUUUAUCGGUCAGUAUUCAGCCAUCAGCUUCCAUC ....((((.....(((((.(..(((.(.((((...(((((...)))))...)))).).)))..).)))))...........(((....)))..))))...............((((((.(((....))))))))).(((..((....))..)))..(((((...((((((((.....))))))...))...)))))............. (-62.40) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GAUGGAAGCUGAUGGCUGAAUACUGACCGAUAAAUUACAUCAGGCUUACAGCCCUCCGAACAAGGUUGGACGGGACCCCACCCUUACGGGAUGGGGCUGAAAUAUAUCGGGUUUGCAGCCCUUUCGGGUAAUGGUUAUGUACAGGGUACACGGUGUCCGAAUGCAAAUUUCGCAAAUGAACGCAGUACGCACCGUAGAGACGCAAUGAU ....(...((.((((.((..(((((.((((((.(((...((((........((((((((......))))).))).((((((((....))).)))))))))))).))))))((((((.((((....))))........((((..((..((...))..))...))))......)))))).....)))))..))))))..))...)...... (-62.10) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 857 140 91 55 6 tfor:TF2840|5end_hypothetical_protein_3031060..3031290_POSITIVE 835 160 111 84 29 tfor:TF2547|5end_hypothetical_protein_2730664..2730894_POSITIVE 814 158 111 231 180 tfor:TF0678|5end_hypothetical_protein_721103..721333_POSITIVE 798 130 83 213 166 tfor:TF0822|5end_hypothetical_protein_880487..880717_POSITIVE 798 130 83 213 166 tfor:TF0821|5end_hypothetical_protein_879879..880109_POSITIVE 634 114 71 223 178 tfor:TF1655|5end_long-chain-fatty-acid-CoA_ligase_1769279..1769509_POSITIVE 606 117 71 231 178 tfor:TF2191|5end_hypothetical_protein_2365934..2366164_POSITIVE 580 70 26 77 24 tfor:TF1183|5end_hypothetical_protein_1268391..1268621_POSITIVE 571 200 151 204 149 tfor:TF3105|5end_hypothetical_protein_3338068..3338298_POSITIVE 548 170 122 226 175 tfor:TF2745|5end_hypothetical_protein_2927562..2927792_POSITIVE 548 175 131 165 119 tfor:TF1274|5end_hypothetical_protein_1357621..1357851_POSITIVE 542 175 131 65 19 tfor:TF3003|5end_hypothetical_protein_3220382..3220612_POSITIVE 500 170 138 34 1 tfor:TF0820|5end_hypothetical_protein_879656..879886_POSITIVE 500 170 122 187 137 tfor:TF0499|5end_hypothetical_protein_526257..526487_POSITIVE 483 58 16 96 45 tfor:TF1184|5end_hypothetical_protein_1269004..1269234_POSITIVE 476 100 51 221 171 tfor:TF0679|5end_hypothetical_protein_721155..721385_POSITIVE 463 175 131 94 49 tfor:TF2640|5end_hypothetical_protein_2809106..2809336_POSITIVE 461 80 32 114 74 tfor:TF1660|5end_hypothetical_protein_1775269..1775499_POSITIVE 459 160 111 201 148 tfor:TF1275|5end_transcription-repair_coupling_factor_1357866..1358096_POSITIVE 452 160 111 87 34 tfor:TF1516|5end_transposase_fragment_1626407..1626637_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 638 179 131 89 44 tfor:TF1762|3end_hypothetical_protein_1899648..1899798_POSITIVE 582 179 131 62 19 tfor:TF0018|3end_hypothetical_protein_12091..12241_POSITIVE 551 170 122 71 25 tfor:TF2922|3end_surface_antigen_BspA_3121538..3121688_POSITIVE 540 175 131 86 42 tfor:TF0344|3end_hypothetical_protein_366963..367113_POSITIVE 540 175 131 46 2 tfor:TF0343|3end_hypothetical_protein_366923..367073_POSITIVE 522 173 131 77 33 tfor:TF2350|3end_hypothetical_protein_2515364..2515514_POSITIVE 520 180 133 53 1 tfor:TF0677|3end_ATP-binding_protein,_Mrp/Nbp35_family,_involved_in_chromosome_partitioning_720982..721132_POSITIVE 503 198 152 54 5 tfor:TF2047|3end_hypothetical_protein_2210298..2210448_POSITIVE 492 175 136 53 20 tfor:TF2169|3end_hypothetical_protein_2345354..2345504_POSITIVE 492 175 136 42 9 tfor:TF0361|3end_hypothetical_protein_379810..379960_POSITIVE 459 160 111 150 97 tfor:TF1274|3end_hypothetical_protein_1357895..1358045_POSITIVE 452 169 121 54 1 tfor:TF1743|3end_conserved_hypothetical_protein_1877909..1878059_POSITIVE 450 160 111 51 2 tfor:TF2172|3end_hypothetical_protein_2346135..2346285_POSITIVE 437 170 144 27 1 tfor:TF0819|3end_phosphoglycerate_mutase_879729..879879_POSITIVE 436 167 122 151 108 tfor:TF2025|3end_hypothetical_protein_2182180..2182330_POSITIVE 433 193 152 53 5 tfor:TF0339|3end_hypothetical_protein_363747..363897_POSITIVE 430 170 121 83 27 tfor:TF1585|3end_hypothetical_protein_1690610..1690760_POSITIVE 430 170 121 76 20 tfor:TF1584|3end_hypothetical_protein_1690603..1690753_POSITIVE 429 196 154 44 2 tfor:TF1839|3end_conserved_hypothetical_protein_1989699..1989849_POSITIVE 412 175 136 42 9 tfor:TF0349|3end_hypothetical_protein_370719..370869_POSITIVE