Origin IGS: tattttatatcaggcttacagccctccgcaaggagattgctttttccccagcgcttcgcattgggctgaattaacacgccctttcagggctcttacagcagtcagccgtcagttccgcattcctccttggaagggggggaagggggatgttcatatcggcatta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taatgccgatatgaacatcccccttcccccccttccaaggaggaatgcggaactgacggctgactgctgtaagagccctgaaagggcgtgttaattcagcccaatgcgaagcgctggggaaaaagcaatctccttgcggagggctgtaagcctgatataaaata Mask Tandem Repeat Region ================================================ tattttatatcaggcttacagccctccgcaaggagattgctttttccccagcgcttcgcattgggctgaattaacacgccctttcagggctcttacagcagtcagccgtcagttccgcattcctccttggaagggggggaagggggatgttcatatcggcatta Find is-nt database================================================ Query_seq: TF0344:TF0346|TF0344:TF0346:hypothetical protein:hypothetical protein:->->:367024..367187 164 tattttatatcaggcttacagccctccgcaaggagattgctttttccccagcgcttcgcattgggctgaattaacacgccctttcagggctcttacagcagtcagccgtcagttccgcattcctccttggaagggggggaagggggatgttcatatcggcatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF0344:TF0346|TF0344:TF0346:hypothetical protein:hypothetical protein:->->:367024..367187 164 tattttatatcaggcttacagccctccgcaaggagattgctttttccccagcgcttcgcattgggctgaattaacacgccctttcagggctcttacagcagtcagccgtcagttccgcattcctccttggaagggggggaagggggatgttcatatcggcatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF0344:TF0346|TF0344:TF0346:hypothetical protein:hypothetical protein:->->:367024..367187 164 tattttatatcaggcttacagccctccgcaaggagattgctttttccccagcgcttcgcattgggctgaattaacacgccctttcagggctcttacagcagtcagccgtcagttccgcattcctccttggaagggggggaagggggatgttcatatcggcatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tattttatatcaggcttacagccctccgcaaggagattgctttttccccagcgcttcgcattgggctgaattaacacgccctttcagggctcttacagcagtcagccgtcagttccgcattcctccttggaagggggggaagggggatgttcatatcggcatta Predict ORF larger than 30AA ================================================ Protein_Len: 31 Strand: + Start: 70 End: 162 ..................................................................... M N T P F Q G S Y S S Q P S V P H S S L E G G E G G C S Y R H .. Protein_Len: 54 Strand: + Start: 2 End: 163 . M L Y Q A Y S P P Q G D C F F P S A S H W A E L T R P F R A L T A V S R Q F R I P P W K G G K G D V H I G I . tattttatatcaggcttacagccctccgcaaggagattgctttttccccagcgcttcgcattgggctgaattaacacgccctttcagggctcttacagcagtcagccgtcagttccgcattcctccttggaagggggggaagggggatgttcatatcggcatta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tattttatatcaggcttacagccctccgcaaggagattgctttttccccagcgcttcgcattgggctgaattaacacgccctttcagggctcttacagcagtcagccgtcagttccgcattcctccttggaagggggggaagggggatgttcatatcggcatta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF0344:TF0346|TF0344:TF0346:hypothetical protein:hypothetical protein:->->:367024..367187 164 tattttatatcaggcttacagccctccgcaaggagattgctttttccccagcgcttcgcattgggctgaattaacacgccctttcagggctcttacagcagtcagccgtcagttccgcattcctccttggaagggggggaagggggatgttcatatcggcatta Intra-Species Hit: Count: 6 Min: 1 Max: 132 Len: 132 Subject: tfor_TF0344_TF0346|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[367024,367187]|164 HSP 1 e-value: 3.0E-69 bit: 262.0 Len: 132 Query Start:1 Query End:132 Subject Strand: POSITIVE Subject Start: 1 Subject End: 132 tattttatatcaggcttacagccctccgcaaggagattgctttttccccagcgcttcgcattgggctgaattaacacgccctttcagggctcttacagcagtcagccgtcagttccgcattcctccttggaa................................ tattttatatcaggcttacagccctccgcaaggagattgctttttccccagcgcttcgcattgggctgaattaacacgccctttcagggctcttacagcagtcagccgtcagttccgcattcctccttggaa................................ Subject: tfor_TF2165_TF2166|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[2341983,2342254]|272 HSP 1 e-value: 1.0E-28 bit: 127.0 Len: 131 Query Start:2 Query End:132 Subject Strand: POSITIVE Subject Start: 107 Subject End: 240 .attttatatcaggcttacagccctccgcaagga---gattgctttttccccagcgcttcgcattgggctgaattaacacgccctttcagggctcttacagcagtcagccgtcagttccgcattcctccttggaa................................ .attttatatcaggcttacagcccttcgccgagacgcgatgcctttttccccagcgcttcgcattgggctgaattaatgcgcccctgcggagctgttacagcggtcagccgtcagttccgcattcctccttggaa................................ Subject: tfor_TF2047_TF2048|hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[2210359,2210782]|424 HSP 1 e-value: 4.0E-19 bit: 95.6 Len: 103 Query Start:10 Query End:112 Subject Strand: POSITIVE Subject Start: 68 Subject End: 173 .........tcaggcttacagccctccgcaagga---gattgctttttccccagcgcttcgcattgggctgaattaacacgccctttcagggctcttacagcagtcagccgtcag.................................................... .........tcaggcttacagccctccgccgggatgcgatgcctttttccccaacgcttcgcattgggctgaattatctcgccctttcagggctctttctgcaatcagcagtcag.................................................... Subject: tfor_TF2169_TF2171|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[2345415,2345624]|210 HSP 1 e-value: 2.0E-15 bit: 83.8 Len: 74 Query Start:59 Query End:132 Subject Strand: POSITIVE Subject Start: 105 Subject End: 178 ..........................................................cattgggctgaattaacacgccctttcagggctcttacagcagtcagccgtcagttccgcattcctccttggaa................................ ..........................................................cattgggctgaattaatgcgcccctgcggagctgttacagcggtcagccgtcagttccgcattcctccttggaa................................ HSP 2 e-value: 9.0E-8 bit: 58.0 Len: 29 Query Start:2 Query End:30 Subject Strand: POSITIVE Subject Start: 35 Subject End: 63 .attttatatcaggcttacagccctccgca...................................................................................................................................... .attttatatcaggcttacagccctccgca...................................................................................................................................... Subject: tfor_TF0365_TF0366|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[383213,383703]|491 HSP 1 e-value: 2.0E-15 bit: 83.8 Len: 74 Query Start:59 Query End:132 Subject Strand: POSITIVE Subject Start: 386 Subject End: 459 ..........................................................cattgggctgaattaacacgccctttcagggctcttacagcagtcagccgtcagttccgcattcctccttggaa................................ ..........................................................cattgggctgaattaacgcaccccttcggagctgttacagtggtcagccgtcagttccgcattcctccttggaa................................ HSP 2 e-value: 9.0E-8 bit: 58.0 Len: 29 Query Start:2 Query End:30 Subject Strand: POSITIVE Subject Start: 316 Subject End: 344 .attttatatcaggcttacagccctccgca...................................................................................................................................... .attttatatcaggcttacagccctccgca...................................................................................................................................... Subject: tfor_TF0361_TF0362|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[379871,380091]|221 HSP 1 e-value: 4.0E-13 bit: 75.8 Len: 74 Query Start:59 Query End:132 Subject Strand: POSITIVE Subject Start: 116 Subject End: 189 ..........................................................cattgggctgaattaacacgccctttcagggctcttacagcagtcagccgtcagttccgcattcctccttggaa................................ ..........................................................cattgggctgaattaacgcatcccttcggagctgttacagtggtcagccgtcagttccgcattcctccttggaa................................ HSP 2 e-value: 9.0E-8 bit: 58.0 Len: 29 Query Start:2 Query End:30 Subject Strand: POSITIVE Subject Start: 46 Subject End: 74 .attttatatcaggcttacagccctccgca...................................................................................................................................... .attttatatcaggcttacagccctccgca...................................................................................................................................... Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAUUUUAUAUCAGGCUUACAGCCCUCCGCAAGGAGAUUGCUUUUUCCCCAGCGCUUCGCAUUGGGCUGAAUUAACACGCCCUUUCAGGGCUCUUACAGCAGUCAGCCGUCAGUUCCGCAUUCCUCCUUGGAA ............((((.((....((((....))))..((((.......((((.(........).)))).........((((.....))))......)))))).))))............((((.....)))) (-37.20) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUCCAAGGAGGAAUGCGGAACUGACGGCUGACUGCUGUAAGAGCCCUGAAAGGGCGUGUUAAUUCAGCCCAAUGCGAAGCGCUGGGGAAAAAGCAAUCUCCUUGCGGAGGGCUGUAAGCCUGAUAUAAAAUA (((((((((((..(((....((.(((((.....))))).))..((((....((((.((......))))))...(((...))).)))).....))).)))))))).)))((((.....))))........... (-43.00) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 953 90 41 189 139 tfor:TF2824|5end_nucleoside-transporting_protein_nupG_3014701..3014931_POSITIVE 913 90 41 198 149 tfor:TF0828|5end_hypothetical_protein_887090..887320_POSITIVE 909 90 41 188 139 tfor:TF1183|5end_hypothetical_protein_1268391..1268621_POSITIVE 895 90 41 115 66 tfor:TF2191|5end_hypothetical_protein_2365934..2366164_POSITIVE 895 90 41 192 143 tfor:TF1186|5end_hypothetical_protein_1269568..1269798_POSITIVE 895 90 41 77 28 tfor:TF0822|5end_hypothetical_protein_880487..880717_POSITIVE 848 70 21 198 143 tfor:TF2478|5end_hypothetical_protein_2655375..2655605_POSITIVE 846 60 11 220 169 tfor:TF0823|5end_conserved_hypothetical_protein_880660..880890_POSITIVE 829 59 11 73 23 tfor:TF0679|5end_hypothetical_protein_721155..721385_POSITIVE 820 90 41 77 29 tfor:TF0821|5end_hypothetical_protein_879879..880109_POSITIVE 819 90 41 215 166 tfor:TF1184|5end_hypothetical_protein_1269004..1269234_POSITIVE 764 60 11 219 167 tfor:TF1187|5end_hypothetical_protein_1269625..1269855_POSITIVE 762 99 52 184 137 tfor:TF2517|5end_hypothetical_protein_2695089..2695319_POSITIVE 755 90 43 122 65 tfor:TF0538|5end_hypothetical_protein_566891..567121_POSITIVE 755 90 43 115 58 tfor:TF0539|5end_hypothetical_protein_566884..567114_POSITIVE 747 59 11 65 14 tfor:TF3107|5end_hypothetical_protein_3338459..3338689_POSITIVE 704 59 11 69 17 tfor:TF1716|5end_hypothetical_protein_1837442..1837672_POSITIVE 692 90 41 63 3 tfor:TF0678|5end_hypothetical_protein_721103..721333_POSITIVE 691 60 11 190 140 tfor:TF2036|5end_possible_pyruvate_formate-lyase_activating_enzyme_2196302..2196532_POSITIVE 596 99 52 131 79 tfor:TF0534|5end_hypothetical_protein_559509..559739_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 895 90 41 107 58 tfor:TF1185|3end_hypothetical_protein_1269563..1269713_POSITIVE 820 90 41 60 12 tfor:TF0820|3end_hypothetical_protein_879942..880092_POSITIVE 819 90 41 69 20 tfor:TF1182|3end_hypothetical_protein_1268938..1269088_POSITIVE 747 59 11 134 83 tfor:TF3106|3end_hypothetical_protein_3338608..3338758_POSITIVE 739 90 41 81 23 tfor:TF2840|3end_hypothetical_protein_3031289..3031439_POSITIVE 738 60 11 115 63 tfor:TF2547|3end_hypothetical_protein_2730947..2731097_POSITIVE 596 99 52 61 9 tfor:TF0533|3end_hypothetical_protein_559519..559669_POSITIVE 553 59 11 141 89 tfor:TF1405|3end_conserved_hypothetical_protein_1481819..1481969_POSITIVE 486 67 32 40 4 tfor:TF0370|3end_hypothetical_protein_386317..386467_POSITIVE 481 53 11 140 89 tfor:TF1274|3end_hypothetical_protein_1357895..1358045_POSITIVE 472 130 81 104 42 tfor:TF0685|3end_hypothetical_protein_731132..731282_POSITIVE 410 70 21 54 4 tfor:TF2172|3end_hypothetical_protein_2346135..2346285_POSITIVE 387 69 25 66 13 tfor:TF2748|3end_ATP-dependent_Clp_protease_2933151..2933301_POSITIVE 384 119 74 79 28 tfor:TF0825|3end_hypothetical_protein_883850..884000_POSITIVE 382 37 10 149 122 tfor:TF0678|3end_hypothetical_protein_721335..721485_POSITIVE 378 49 5 127 75 tfor:TF2816|3end_hypothetical_protein_3007184..3007334_POSITIVE 375 90 45 117 73 tfor:TF2640|3end_hypothetical_protein_2809533..2809683_POSITIVE 375 66 21 62 3 tfor:TF2340|3end_hypothetical_protein_2500915..2501065_POSITIVE 373 90 44 149 98 tfor:TF1590|3end_hypothetical_protein_1706043..1706193_POSITIVE 370 128 98 109 77 tfor:TF1660|3end_hypothetical_protein_1775600..1775750_POSITIVE