Origin IGS: cggtacaaagataagcacttttagcctacggaaacaagataggggctatgaatattttgcggtggtctgccgctccggtacgggctgttgcttacaagcacctctctttccgtgcagaaaacgaatgatgcggcaaggcggtatcaacaattgagcttcaccgtcgtaaaagaaaacagtgaagattacgtcatggtttgtaatttgtgataccggcttattcggaagaattgctctttgctaacgctcgttca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tgaacgagcgttagcaaagagcaattcttccgaataagccggtatcacaaattacaaaccatgacgtaatcttcactgttttcttttacgacggtgaagctcaattgttgataccgccttgccgcatcattcgttttctgcacggaaagagaggtgcttgtaagcaacagcccgtaccggagcggcagaccaccgcaaaatattcatagcccctatcttgtttccgtaggctaaaagtgcttatctttgtaccg Mask Tandem Repeat Region ================================================ cggtacaaagataagcacttttagcctacggaaacaagataggggctatgaatattttgcggtggtctgccgctccggtacgggctgttgcttacaagcacctctctttccgtgcagaaaacgaatgatgcggcaaggcggtatcaacaattgagcttcaccgtcgtaaaagaaaacagtgaagattacgtcatggtttgtaatttgtgataccggcttattcggaagaattgctctttgctaacgctcgttca Find is-nt database================================================ Query_seq: TF0323:TF0324|TF0323:TF0324:NAD-dependent epimerase/dehydratase family protein:conserved hypothetical protein:->->:351114..351367 254 cggtacaaagataagcacttttagcctacggaaacaagataggggctatgaatattttgcggtggtctgccgctccggtacgggctgttgcttacaagcacctctctttccgtgcagaaaacgaatgatgcggcaaggcggtatcaacaattgagcttcaccgtcgtaaaagaaaacagtgaagattacgtcatggtttgtaatttgtgataccggcttattcggaagaattgctctttgctaacgctcgttca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF0323:TF0324|TF0323:TF0324:NAD-dependent epimerase/dehydratase family protein:conserved hypothetical protein:->->:351114..351367 254 cggtacaaagataagcacttttagcctacggaaacaagataggggctatgaatattttgcggtggtctgccgctccggtacgggctgttgcttacaagcacctctctttccgtgcagaaaacgaatgatgcggcaaggcggtatcaacaattgagcttcaccgtcgtaaaagaaaacagtgaagattacgtcatggtttgtaatttgtgataccggcttattcggaagaattgctctttgctaacgctcgttca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF0323:TF0324|TF0323:TF0324:NAD-dependent epimerase/dehydratase family protein:conserved hypothetical protein:->->:351114..351367 254 cggtacaaagataagcacttttagcctacggaaacaagataggggctatgaatattttgcggtggtctgccgctccggtacgggctgttgcttacaagcacctctctttccgtgcagaaaacgaatgatgcggcaaggcggtatcaacaattgagcttcaccgtcgtaaaagaaaacagtgaagattacgtcatggtttgtaatttgtgataccggcttattcggaagaattgctctttgctaacgctcgttca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 cggtacaaagataagcacttttagcctacggaaacaagataggggctatgaatattttgcggtggtctgccgctccggtacgggctgttgcttacaagcacctctctttccgtgcagaaaacgaatgatgcggcaaggcggtatcaacaattgagcttcaccgtcgtaaaagaaaacagtgaagattacgtcatggtttgtaatttgtgataccggcttattcggaagaattgctctttgctaacgctcgttca Predict ORF larger than 30AA ================================================ Protein_Len: 47 Strand: + Start: 11 End: 151 .......... M S T F S L R K Q D R G Y E Y F A V V C R S G T G C C L Q A P L F P C R K R M M R Q G G I N N ....................................................................................................... Protein_Len: 52 Strand: + Start: 52 End: 207 ................................................... M F C G G L P L R Y G L L L T S T S L S V Q K T N D A A R R Y Q Q L S F T V V K E N S E D Y V M V C N L ............................................... Protein_Len: 60 Strand: - Start: 49 End: 231 ................................................ Y K A T T Q R E P V P Q Q K C A G R K G H L F R I I R C P P I L L Q A E G D Y F F V T F I V D H N T I Q S V P K N P L M ....................... cggtacaaagataagcacttttagcctacggaaacaagataggggctatgaatattttgcggtggtctgccgctccggtacgggctgttgcttacaagcacctctctttccgtgcagaaaacgaatgatgcggcaaggcggtatcaacaattgagcttcaccgtcgtaaaagaaaacagtgaagattacgtcatggtttgtaatttgtgataccggcttattcggaagaattgctctttgctaacgctcgttca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ cggtacaaagataagcacttttagcctacggaaacaagataggggctatgaatattttgcggtggtctgccgctccggtacgggctgttgcttacaagcacctctctttccgtgcagaaaacgaatgatgcggcaaggcggtatcaacaattgagcttcaccgtcgtaaaagaaaacagtgaagattacgtcatggtttgtaatttgtgataccggcttattcggaagaattgctctttgctaacgctcgttca Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF0323:TF0324|TF0323:TF0324:NAD-dependent epimerase/dehydratase family protein:conserved hypothetical protein:->->:351114..351367 254 cggtacaaagataagcacttttagcctacggaaacaagataggggctatgaatattttgcggtggtctgccgctccggtacgggctgttgcttacaagcacctctctttccgtgcagaaaacgaatgatgcggcaaggcggtatcaacaattgagcttcaccgtcgtaaaagaaaacagtgaagattacgtcatggtttgtaatttgtgataccggcttattcggaagaattgctctttgctaacgctcgttca Intra-Species Hit: Count: 1 Min: 1 Max: 254 Len: 254 Subject: tfor_TF0323_TF0324|NAD-dependent epimerase/dehydratase family protein:conserved hypothetical protein|POSITIVE:POSITIVE|[351114,351367]|254 HSP 1 e-value: 1.0E-142 bit: 504.0 Len: 254 Query Start:1 Query End:254 Subject Strand: POSITIVE Subject Start: 1 Subject End: 254 cggtacaaagataagcacttttagcctacggaaacaagataggggctatgaatattttgcggtggtctgccgctccggtacgggctgttgcttacaagcacctctctttccgtgcagaaaacgaatgatgcggcaaggcggtatcaacaattgagcttcaccgtcgtaaaagaaaacagtgaagattacgtcatggtttgtaatttgtgataccggcttattcggaagaattgctctttgctaacgctcgttca cggtacaaagataagcacttttagcctacggaaacaagataggggctatgaatattttgcggtggtctgccgctccggtacgggctgttgcttacaagcacctctctttccgtgcagaaaacgaatgatgcggcaaggcggtatcaacaattgagcttcaccgtcgtaaaagaaaacagtgaagattacgtcatggtttgtaatttgtgataccggcttattcggaagaattgctctttgctaacgctcgttca Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CGGUACAAAGAUAAGCACUUUUAGCCUACGGAAACAAGAUAGGGGCUAUGAAUAUUUUGCGGUGGUCUGCCGCUCCGGUACGGGCUGUUGCUUACAAGCACCUCUCUUUCCGUGCAGAAAACGAAUGAUGCGGCAAGGCGGUAUCAACAAUUGAGCUUCACCGUCGUAAAAGAAAACAGUGAAGAUUACGUCAUGGUUUGUAAUUUGUGAUACCGGCUUAUUCGGAAGAAUUGCUCUUUGCUAACGCUCGUUCA .(((..((((.......))))..))).((((((...(((.(((.(((.((........((((..((((((((...))))..))))..))))...))))).))).))))))))).........((((((.(((((((((((((((((.(((.....((((((..((......)).....)))))).(((((........))))).)))))))))))((..((((....)))).)).))))))...))))))))). (-74.00) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGAACGAGCGUUAGCAAAGAGCAAUUCUUCCGAAUAAGCCGGUAUCACAAAUUACAAACCAUGACGUAAUCUUCACUGUUUUCUUUUACGACGGUGAAGCUCAAUUGUUGAUACCGCCUUGCCGCAUCAUUCGUUUUCUGCACGGAAAGAGAGGUGCUUGUAAGCAACAGCCCGUACCGGAGCGGCAGACCACCGCAAAAUAUUCAUAGCCCCUAUCUUGUUUCCGUAGGCUAAAAGUGCUUAUCUUUGUACCG .(((.(((((...((.....))............((((.(((((((((((.((((..........)))).((((((((((.........)))))))))).....))).)))))))).)))).))).)).))).((((((....))))))...(((((..(((((((..((((..(((.(((((((.......))))..((((...((((...))))..)))))))))))))).....)))))))....))))). (-65.00) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 482 90 41 217 164 tfor:TF2329|5end_conserved_hypothetical_protein_2488324..2488554_POSITIVE 455 93 58 53 20 tfor:TF0910|5end_Tn5520-like_integrase_(transfer_factor)_952499..952729_POSITIVE 449 110 61 200 155 tfor:TF2181|5end_conserved_hypothetical_protein_2353870..2354100_POSITIVE 426 110 61 199 151 tfor:TF1785|5end_histidine_ammonia-lyase_1924494..1924724_POSITIVE 415 87 44 183 128 tfor:TF2333|5end_signal_peptidase_I_2492560..2492790_POSITIVE 404 100 53 198 159 tfor:TF0534|5end_hypothetical_protein_559509..559739_POSITIVE 395 105 62 77 42 tfor:TF0816|5end_hypothetical_protein_874217..874447_POSITIVE 394 92 54 208 164 tfor:TF1812|5end_tryptophan_synthase,_beta_chain_1952696..1952926_POSITIVE 383 89 42 173 112 tfor:TF2654|5end_arginyl-tRNA_synthetase_2822692..2822922_POSITIVE 383 88 41 155 93 tfor:TF2413|5end_hypothetical_protein_2590376..2590606_POSITIVE 381 120 72 67 3 tfor:TF0090|5end_conserved_hypothetical_protein_96285..96515_POSITIVE 377 140 91 51 3 tfor:TF2876|5end_cytosine_deaminase_3065144..3065374_POSITIVE 376 85 42 49 7 tfor:TF2833|5end_lipid_A_disaccharide_synthase_3023973..3024203_POSITIVE 372 107 61 68 21 tfor:TF2446|5end_riboflavin-specific_deaminase_2622370..2622600_POSITIVE 371 115 73 193 138 tfor:TF2259|5end_transfer_region-related_protein,_TraK_2429577..2429807_POSITIVE 371 167 123 182 136 tfor:TF2210|5end_hypothetical_protein_2384111..2384341_POSITIVE 371 84 41 188 141 tfor:TF1352|5end_polysaccharide_deacetylase-like_protein_1432006..1432236_POSITIVE 369 149 103 221 177 tfor:TF0441|5end_Na+-transporting_NADH:ubiquinone_oxidoreductase,_subunit_3_464614..464844_POSITIVE 367 86 58 213 188 tfor:TF2918|5end_hypothetical_protein_3114084..3114314_POSITIVE 367 117 71 198 140 tfor:TF1599|5end_ribonuclease_P_protein_component_1713564..1713794_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 405 119 71 89 24 tfor:TF2911|3end_hypothetical_protein_3106640..3106790_POSITIVE 404 100 53 128 89 tfor:TF0533|3end_hypothetical_protein_559519..559669_POSITIVE 383 88 41 92 30 tfor:TF2412|3end_outer_membrane_protein_2590393..2590543_POSITIVE 377 140 91 51 3 tfor:TF2874|3end_conserved_hypothetical_protein_3065224..3065374_POSITIVE 369 99 57 151 102 tfor:TF1954|3end_long-chain-fatty-acid--CoA_ligase_2106940..2107090_POSITIVE 369 109 63 79 18 tfor:TF0038|3end_conserved_hypothetical_protein_41213..41363_POSITIVE 368 87 53 54 9 tfor:TF0214|3end_DNA_recombination_and_repair_protein,_RecN_241571..241721_POSITIVE 365 141 102 40 1 tfor:TF1818|3end_conserved_hypothetical_protein_1958754..1958904_POSITIVE 362 116 73 69 24 tfor:TF1596|3end_hypothetical_protein_1712723..1712873_POSITIVE 359 89 42 56 3 tfor:TF1474|3end_6-phosphogluconolactonase_1568123..1568273_POSITIVE 357 247 205 67 27 tfor:TF0612|3end_conserved_hypothetical_protein_646137..646287_POSITIVE 356 87 42 53 4 tfor:TF1917|3end_hypothetical_protein_2070244..2070394_POSITIVE 355 90 42 151 94 tfor:TF1282|3end_tRNA_delta-2_isopentenylpyrophosphate_transferase_1367431..1367581_POSITIVE 354 117 75 67 21 tfor:TF0300|3end_hypothetical_protein_325541..325691_POSITIVE 354 85 45 145 108 tfor:TF0240|3end_conserved_hypothetical_protein;_possible_gycosyltransferase_I_family_protein_271407..271557_POSITIVE 353 89 42 78 32 tfor:TF1709|3end_conserved_hypothetical_protein_1829710..1829860_POSITIVE 352 87 41 136 84 tfor:TF3087|3end_conserved_hypothetical_protein_3322582..3322732_POSITIVE 349 97 61 72 14 tfor:TF1264|3end_possible_transposase_1352341..1352491_POSITIVE 347 123 81 145 99 tfor:TF1801|3end_conserved_hypothetical_protein;_possible_TPR-repeat-containing_protein_1945669..1945819_POSITIVE 346 88 43 124 80 tfor:TF2336|3end_conserved_hypothetical_protein_2498159..2498309_POSITIVE