Origin IGS: acattatatttatatatgctgcgaaatctgatgcctgaactttttcagaggaggcgtgttgtttttatttgcttcacaaaggtagaaaataatttttgaaggcctacggattggaagatatttgtta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taacaaatatcttccaatccgtaggccttcaaaaattattttctacctttgtgaagcaaataaaaacaacacgcctcctctgaaaaagttcaggcatcagatttcgcagcatatataaatataatgt Mask Tandem Repeat Region ================================================ acattatatttatatatgctgcgaaatctgatgcctgaactttttcagaggaggcgtgttgtttttatttgcttcacaaaggtagaaaataatttttgaaggcctacggattggaagatatttgtta Find is-nt database================================================ Query_seq: TF0321:TF0322|TF0321:TF0322:glycosyltransferase:possible YngK protein:->->:348358..348484 127 acattatatttatatatgctgcgaaatctgatgcctgaactttttcagaggaggcgtgttgtttttatttgcttcacaaaggtagaaaataatttttgaaggcctacggattggaagatatttgtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF0321:TF0322|TF0321:TF0322:glycosyltransferase:possible YngK protein:->->:348358..348484 127 acattatatttatatatgctgcgaaatctgatgcctgaactttttcagaggaggcgtgttgtttttatttgcttcacaaaggtagaaaataatttttgaaggcctacggattggaagatatttgtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF0321:TF0322|TF0321:TF0322:glycosyltransferase:possible YngK protein:->->:348358..348484 127 acattatatttatatatgctgcgaaatctgatgcctgaactttttcagaggaggcgtgttgtttttatttgcttcacaaaggtagaaaataatttttgaaggcctacggattggaagatatttgtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 acattatatttatatatgctgcgaaatctgatgcctgaactttttcagaggaggcgtgttgtttttatttgcttcacaaaggtagaaaataatttttgaaggcctacggattggaagatatttgtta Predict ORF larger than 30AA ================================================ Protein_Len: 33 Strand: - Start: 13 End: 111 ............ I H Q S I Q H R F K K L P P T N N K N A E C L Y F I I K S P R R M ................ acattatatttatatatgctgcgaaatctgatgcctgaactttttcagaggaggcgtgttgtttttatttgcttcacaaaggtagaaaataatttttgaaggcctacggattggaagatatttgtta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ acattatatttatatatgctgcgaaatctgatgcctgaactttttcagaggaggcgtgttgtttttatttgcttcacaaaggtagaaaataatttttgaaggcctacggattggaagatatttgtta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF0321:TF0322|TF0321:TF0322:glycosyltransferase:possible YngK protein:->->:348358..348484 127 acattatatttatatatgctgcgaaatctgatgcctgaactttttcagaggaggcgtgttgtttttatttgcttcacaaaggtagaaaataatttttgaaggcctacggattggaagatatttgtta Intra-Species Hit: Count: 1 Min: 1 Max: 127 Len: 127 Subject: tfor_TF0321_TF0322|glycosyltransferase:possible YngK protein|POSITIVE:POSITIVE|[348358,348484]|127 HSP 1 e-value: 2.0E-66 bit: 252.0 Len: 127 Query Start:1 Query End:127 Subject Strand: POSITIVE Subject Start: 1 Subject End: 127 acattatatttatatatgctgcgaaatctgatgcctgaactttttcagaggaggcgtgttgtttttatttgcttcacaaaggtagaaaataatttttgaaggcctacggattggaagatatttgtta acattatatttatatatgctgcgaaatctgatgcctgaactttttcagaggaggcgtgttgtttttatttgcttcacaaaggtagaaaataatttttgaaggcctacggattggaagatatttgtta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ ACAUUAUAUUUAUAUAUGCUGCGAAAUCUGAUGCCUGAACUUUUUCAGAGGAGGCGUGUUGUUUUUAUUUGCUUCACAAAGGUAGAAAAUAAUUUUUGAAGGCCUACGGAUUGGAAGAUAUUUGUUA .............((((.((....((((((..((((..............((((((.((.......)).)))))).(((((((........))))))).))))...))))))...))))))...... (-20.70) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAACAAAUAUCUUCCAAUCCGUAGGCCUUCAAAAAUUAUUUUCUACCUUUGUGAAGCAAAUAAAAACAACACGCCUCCUCUGAAAAAGUUCAGGCAUCAGAUUUCGCAGCAUAUAUAAAUAUAAUGU ..........(((((((...(((((................)))))..))).))))................((((...((.....))...))))..........(((..((((....))))..))) (-14.39) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 330 57 18 78 41 tfor:TF1275|5end_transcription-repair_coupling_factor_1357866..1358096_POSITIVE 326 64 21 134 84 tfor:TF1475|5end_glucose-6-phosphate_1-dehydrogenase_1568034..1568264_POSITIVE 314 63 22 202 151 tfor:TF2752|5end_endonuclease_III_2936781..2937011_POSITIVE 300 60 11 49 10 tfor:TF2218|5end_two-component_system_response_regulator_2390162..2390392_POSITIVE 298 114 75 160 116 tfor:TF2447|5end_lipoprotein_2623549..2623779_POSITIVE 297 60 14 216 159 tfor:TF0591|5end_RNA_polymerase,_ECF-type_sigma_factor_624578..624808_POSITIVE 296 80 32 54 11 tfor:TF0781|5end_serine_protease_inhibitor_834470..834700_POSITIVE 296 56 17 110 54 tfor:TF0546|5end_conserved_hypothetical_protein_576380..576610_POSITIVE 294 60 14 57 21 tfor:TF2396|5end_dimethyladenosine_transferase_2567725..2567955_POSITIVE 293 77 33 65 30 tfor:TF2785|5end_RNA_polymerase_sigma_factor,_ECF_subfamily_(sigma-70)_2969277..2969507_POSITIVE 291 58 18 225 180 tfor:TF2745|5end_hypothetical_protein_2927562..2927792_POSITIVE 291 56 16 230 190 tfor:TF2643|5end_possible_O-antigen_transporter_2811744..2811974_POSITIVE 290 59 11 209 149 tfor:TF1928|5end_conserved_hypothetical_protein_2075529..2075759_POSITIVE 289 62 21 190 143 tfor:TF2318|5end_conserved_hypothetical_protein_2475603..2475833_POSITIVE 286 60 17 184 139 tfor:TF1067|5end_conserved_hypothetical_protein_1136867..1137097_POSITIVE 281 59 14 203 148 tfor:TF2186|5end_outer_membrane_protein_2360291..2360521_POSITIVE 280 58 14 93 52 tfor:TF0695|5end_possible_phosphatidylglycerophosphate_synthase_739021..739251_POSITIVE 279 57 20 146 111 tfor:TF2950|5end_chloromuconate_cycloisomerase_3157399..3157629_POSITIVE 279 60 19 106 64 tfor:TF2477|5end_phosphatase_2654229..2654459_POSITIVE 279 70 22 142 82 tfor:TF0962|5end_possible_amidohydrolase_1011593..1011823_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 326 64 21 143 93 tfor:TF1474|3end_6-phosphogluconolactonase_1568123..1568273_POSITIVE 298 114 75 51 7 tfor:TF2446|3end_riboflavin-specific_deaminase_2623520..2623670_POSITIVE 296 56 17 103 47 tfor:TF0545|3end_glucose-inhibited_division_protein_B_576453..576603_POSITIVE 289 62 32 141 109 tfor:TF1607|3end_conserved_hypothetical_protein_1725838..1725988_POSITIVE 286 60 17 95 50 tfor:TF1066|3end_hypothetical_protein_1136858..1137008_POSITIVE 280 58 14 93 52 tfor:TF0694|3end_possible_hemolysin,_acyltransferase_family_739101..739251_POSITIVE 279 57 20 146 111 tfor:TF2949|3end_dihydroxynaphthoate_synthase_(naphthoate_synthase)_3157479..3157629_POSITIVE 279 70 22 142 82 tfor:TF0961|3end_translation_initiation_inhibitor,_endoribonuclease_activity_1011673..1011823_POSITIVE 274 61 22 59 22 tfor:TF0141|3end_exodeoxyribonuclease_VII,_large_subunit_149830..149980_POSITIVE 270 56 16 147 94 tfor:TF2751|3end_phenylalanyl-tRNA_synthetase,_alpha_subunit_2936815..2936965_POSITIVE 268 58 20 150 113 tfor:TF2924|3end_DNA-binding_response_regulator/sensor_histidine_kinase_3125618..3125768_POSITIVE 267 76 35 101 56 tfor:TF1962|3end_TatD-related_deoxyribonuclease,_Mg-dependent_DNase_2113297..2113447_POSITIVE 265 58 18 30 1 tfor:TF3124|3end_dTDP-glucose_4,6-dehydratase_3359883..3360033_POSITIVE 265 64 28 34 1 tfor:TF1274|3end_hypothetical_protein_1357895..1358045_POSITIVE 265 63 45 24 3 tfor:TF0592|3end_epimerase/reductase_626451..626601_POSITIVE 262 60 17 87 44 tfor:TF2019|3end_dipeptidyl_peptidase_VI_2176401..2176551_POSITIVE 260 84 50 104 69 tfor:TF1862|3end_hypothetical_protein_2018784..2018934_POSITIVE 260 79 31 67 19 tfor:TF0392|3end_hypothetical_protein_412945..413095_POSITIVE 259 117 97 105 79 tfor:TF1498|3end_GTP-binding_protein_1598490..1598640_POSITIVE 258 61 22 118 82 tfor:TF0272|3end_outer_membrane_protein_299727..299877_POSITIVE