Origin IGS: tgagtgatccaagacgggaaacaggcttctgaaaaatcactttttctcttgctcttttttacaagttcacgcgctgctatgactatgtcgtgaccgaaatggtcggaaacgagggtca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tgaccctcgtttccgaccatttcggtcacgacatagtcatagcagcgcgtgaacttgtaaaaaagagcaagagaaaaagtgatttttcagaagcctgtttcccgtcttggatcactca Mask Tandem Repeat Region ================================================ tgagtgatccaagacgggaaacaggcttctgaaaaatcactttttctcttgctcttttttacaagttcacgcgctgctatgactatgtcgtgaccgaaatggtcggaaacgagggtca Find is-nt database================================================ Query_seq: TF0294:TF0296|TF0294:TF0296:possible translation factor:hypothetical protein:->->:321963..322080 118 tgagtgatccaagacgggaaacaggcttctgaaaaatcactttttctcttgctcttttttacaagttcacgcgctgctatgactatgtcgtgaccgaaatggtcggaaacgagggtca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF0294:TF0296|TF0294:TF0296:possible translation factor:hypothetical protein:->->:321963..322080 118 tgagtgatccaagacgggaaacaggcttctgaaaaatcactttttctcttgctcttttttacaagttcacgcgctgctatgactatgtcgtgaccgaaatggtcggaaacgagggtca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF0294:TF0296|TF0294:TF0296:possible translation factor:hypothetical protein:->->:321963..322080 118 tgagtgatccaagacgggaaacaggcttctgaaaaatcactttttctcttgctcttttttacaagttcacgcgctgctatgactatgtcgtgaccgaaatggtcggaaacgagggtca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tgagtgatccaagacgggaaacaggcttctgaaaaatcactttttctcttgctcttttttacaagttcacgcgctgctatgactatgtcgtgaccgaaatggtcggaaacgagggtca Predict ORF larger than 30AA ================================================ tgagtgatccaagacgggaaacaggcttctgaaaaatcactttttctcttgctcttttttacaagttcacgcgctgctatgactatgtcgtgaccgaaatggtcggaaacgagggtca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tgagtgatccaagacgggaaacaggcttctgaaaaatcactttttctcttgctcttttttacaagttcacgcgctgctatgactatgtcgtgaccgaaatggtcggaaacgagggtca Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF0294:TF0296|TF0294:TF0296:possible translation factor:hypothetical protein:->->:321963..322080 118 tgagtgatccaagacgggaaacaggcttctgaaaaatcactttttctcttgctcttttttacaagttcacgcgctgctatgactatgtcgtgaccgaaatggtcggaaacgagggtca Intra-Species Hit: Count: 1 Min: 1 Max: 118 Len: 118 Subject: tfor_TF0294_TF0296|possible translation factor:hypothetical protein|POSITIVE:POSITIVE|[321963,322080]|118 HSP 1 e-value: 5.0E-61 bit: 234.0 Len: 118 Query Start:1 Query End:118 Subject Strand: POSITIVE Subject Start: 1 Subject End: 118 tgagtgatccaagacgggaaacaggcttctgaaaaatcactttttctcttgctcttttttacaagttcacgcgctgctatgactatgtcgtgaccgaaatggtcggaaacgagggtca tgagtgatccaagacgggaaacaggcttctgaaaaatcactttttctcttgctcttttttacaagttcacgcgctgctatgactatgtcgtgaccgaaatggtcggaaacgagggtca Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGAGUGAUCCAAGACGGGAAACAGGCUUCUGAAAAAUCACUUUUUCUCUUGCUCUUUUUUACAAGUUCACGCGCUGCUAUGACUAUGUCGUGACCGAAAUGGUCGGAAACGAGGGUCA ..((((......(((.(((((..(((....((((((....))))))....)))..)))))....)))....))))....(((((...((((..((((.....))))..)))).))))) (-26.40) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGACCCUCGUUUCCGACCAUUUCGGUCACGACAUAGUCAUAGCAGCGCGUGAACUUGUAAAAAAGAGCAAGAGAAAAAGUGAUUUUUCAGAAGCCUGUUUCCCGUCUUGGAUCACUCA ((((..((((..((((.....))))..))))....)))).(((((.((.....(((((........))))).((((((....))))))....)))))))(((......)))....... (-28.70) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 377 109 62 137 89 tfor:TF1185|5end_hypothetical_protein_1269238..1269468_POSITIVE 364 106 66 48 1 tfor:TF0987|5end_DNA_repair_protein_1043090..1043320_POSITIVE 343 109 66 47 5 tfor:TF1182|5end_hypothetical_protein_1268565..1268795_POSITIVE 327 100 51 201 148 tfor:TF0693|5end_sigma-54_dependent_transcriptional_regulator_736902..737132_POSITIVE 322 110 63 52 8 tfor:TF1474|5end_6-phosphogluconolactonase_1567342..1567572_POSITIVE 319 106 68 165 121 tfor:TF0864|5end_O-succinylbenzoate--CoA_ligase_914570..914800_POSITIVE 315 105 64 228 182 tfor:TF1937|5end_conserved_hypothetical_protein_2084476..2084706_POSITIVE 315 107 71 63 28 tfor:TF1713|5end_hypothetical_protein_1833442..1833672_POSITIVE 314 109 62 215 167 tfor:TF2746|5end_hypothetical_protein_2927996..2928226_POSITIVE 313 90 41 213 164 tfor:TF0785|5end_biopolymer_transport_protein,_MotA/TolQ/ExbB_proton_channel_family_837810..838040_POSITIVE 309 109 62 137 89 tfor:TF1845|5end_hypothetical_protein_1996620..1996850_POSITIVE 307 108 71 51 5 tfor:TF1919|5end_hypothetical_protein_2070037..2070267_POSITIVE 305 109 62 120 71 tfor:TF2517|5end_hypothetical_protein_2695089..2695319_POSITIVE 303 60 13 176 137 tfor:TF0326|5end_hypothetical_protein_353854..354084_POSITIVE 303 60 13 40 1 tfor:TF0327|5end_hypothetical_protein_353718..353948_POSITIVE 303 96 51 53 10 tfor:TF0085|5end_ABC_transporter,_ATP-binding_protein_91517..91747_POSITIVE 299 105 69 97 63 tfor:TF1743|5end_conserved_hypothetical_protein_1876708..1876938_POSITIVE 298 109 72 212 167 tfor:TF1242|5end_3-methyl-2-oxobutanoate_hydroxymethyltransferase_1326216..1326446_POSITIVE 298 97 52 67 32 tfor:TF1145|5end_anaerobic_C4-dicarboxylate_membrane_transporter_1225799..1226029_POSITIVE 298 105 66 226 188 tfor:TF0457|5end_beta-glucanase_precursor_474099..474329_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 377 109 62 130 82 tfor:TF1184|3end_hypothetical_protein_1269311..1269461_POSITIVE 377 109 62 130 82 tfor:TF1183|3end_hypothetical_protein_1268728..1268878_POSITIVE 317 109 62 130 82 tfor:TF1846|3end_hypothetical_protein_1997015..1997165_POSITIVE 317 109 62 120 72 tfor:TF1845|3end_hypothetical_protein_1997005..1997155_POSITIVE 315 107 71 65 30 tfor:TF1712|3end_conserved_hypothetical_protein_1833524..1833674_POSITIVE 314 109 62 86 38 tfor:TF2745|3end_hypothetical_protein_2927947..2928097_POSITIVE 309 109 62 130 82 tfor:TF1844|3end_hypothetical_protein_1996693..1996843_POSITIVE 309 109 62 104 56 tfor:TF1659|3end_hypothetical_protein_1775209..1775359_POSITIVE 303 96 51 53 10 tfor:TF0084|3end_possible_membrane-fusion_protein_91597..91747_POSITIVE 300 106 68 59 24 tfor:TF2351|3end_acetate_kinase_2516807..2516957_POSITIVE 297 103 62 67 17 tfor:TF1177|3end_conserved_hypothetical_protein;_possible_ribosomal_protein_S1_1264773..1264923_POSITIVE 296 106 65 149 103 tfor:TF1829|3end_2-isopropylmalate_synthase_1978929..1979079_POSITIVE 289 105 64 57 8 tfor:TF2852|3end_conserved_hypothetical_protein_3040221..3040371_POSITIVE 289 108 79 147 111 tfor:TF2530|3end_conserved_hypothetical_protein_2712926..2713076_POSITIVE 288 78 37 88 53 tfor:TF2311|3end_conserved_hypothetical_protein_2471612..2471762_POSITIVE 288 108 63 147 101 tfor:TF1583|3end_conserved_hypothetical_protein;_possible_endonuclease_1690223..1690373_POSITIVE 283 105 61 142 94 tfor:TF3021|3end_FKBP-type_peptidyl-prolyl_cis-trans_isomerase_3246194..3246344_POSITIVE 282 109 62 104 56 tfor:TF0598|3end_hypothetical_protein_629500..629650_POSITIVE 281 97 51 97 46 tfor:TF0673|3end_conserved_hypothetical_protein_717355..717505_POSITIVE 279 108 71 64 16 tfor:TF2736|3end_methylglyoxal_synthase_2918458..2918608_POSITIVE