Origin IGS: taaattccgtttgcagatgagtcgatccggagagcatacgaaaggatttcgtctcttgccggcagaaaaaagagggtgtgtcaaagcgtaatgtttgggcacgccctcttcaggtgtgtaagaaggggcaaaatttcgggacgctatggatgaaggttcgggagcatacggctagtatgcccccaaactttcatctcacgaacaatgagatgtttgaaaggttgtgacaccttgtctctcgatctggaaaaatatgagaaacaccgtta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taacggtgtttctcatatttttccagatcgagagacaaggtgtcacaacctttcaaacatctcattgttcgtgagatgaaagtttgggggcatactagccgtatgctcccgaaccttcatccatagcgtcccgaaattttgccccttcttacacacctgaagagggcgtgcccaaacattacgctttgacacaccctcttttttctgccggcaagagacgaaatcctttcgtatgctctccggatcgactcatctgcaaacggaattta Mask Tandem Repeat Region ================================================ taaattccgtttgcagatgagtcgatccggagagcatacgaaaggatttcgtctcttgccggcagaaaaaagagggtgtgtcaaagcgtaatgtttgggcacgccctcttcaggtgtgtaagaaggggcaaaatttcgggacgctatggatgaaggttcgggagcatacggctagtatgcccccaaactttcatctcacgaacaatgagatgtttgaaaggttgtgacaccttgtctctcgatctggaaaaatatgagaaacaccgtta Find is-nt database================================================ Query_seq: TF0178:TF0179|TF0178:TF0179:ferritin:pyrophosphate-energized proton pump:->->:188284..188552 269 taaattccgtttgcagatgagtcgatccggagagcatacgaaaggatttcgtctcttgccggcagaaaaaagagggtgtgtcaaagcgtaatgtttgggcacgccctcttcaggtgtgtaagaaggggcaaaatttcgggacgctatggatgaaggttcgggagcatacggctagtatgcccccaaactttcatctcacgaacaatgagatgtttgaaaggttgtgacaccttgtctctcgatctggaaaaatatgagaaacaccgtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF0178:TF0179|TF0178:TF0179:ferritin:pyrophosphate-energized proton pump:->->:188284..188552 269 taaattccgtttgcagatgagtcgatccggagagcatacgaaaggatttcgtctcttgccggcagaaaaaagagggtgtgtcaaagcgtaatgtttgggcacgccctcttcaggtgtgtaagaaggggcaaaatttcgggacgctatggatgaaggttcgggagcatacggctagtatgcccccaaactttcatctcacgaacaatgagatgtttgaaaggttgtgacaccttgtctctcgatctggaaaaatatgagaaacaccgtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF0178:TF0179|TF0178:TF0179:ferritin:pyrophosphate-energized proton pump:->->:188284..188552 269 taaattccgtttgcagatgagtcgatccggagagcatacgaaaggatttcgtctcttgccggcagaaaaaagagggtgtgtcaaagcgtaatgtttgggcacgccctcttcaggtgtgtaagaaggggcaaaatttcgggacgctatggatgaaggttcgggagcatacggctagtatgcccccaaactttcatctcacgaacaatgagatgtttgaaaggttgtgacaccttgtctctcgatctggaaaaatatgagaaacaccgtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaattccgtttgcagatgagtcgatccggagagcatacgaaaggatttcgtctcttgccggcagaaaaaagagggtgtgtcaaagcgtaatgtttgggcacgccctcttcaggtgtgtaagaaggggcaaaatttcgggacgctatggatgaaggttcgggagcatacggctagtatgcccccaaactttcatctcacgaacaatgagatgtttgaaaggttgtgacaccttgtctctcgatctggaaaaatatgagaaacaccgtta Predict ORF larger than 30AA ================================================ Protein_Len: 49 Strand: + Start: 4 End: 150 ... M P F A D E S I R R A Y E R I S S L A G R K K R V C Q S V M F G H A L F R C V R R G K I S G R Y G ....................................................................................................................... Protein_Len: 60 Strand: + Start: 36 End: 224 ................................... M R K D F V S C R Q K K E G V S K R N V W A R P L Q V C K K G Q N F G T L W M K V R E H T A S M P P N F H L T N N E M F ............................................. Protein_Len: 34 Strand: + Start: 166 End: 267 ..................................................................................................................................................................... M R L V C P Q T F I S R T M R C L K G C D T L S L D L E K Y E K H R .. Protein_Len: 49 Strand: - Start: 1 End: 147 L N R K C I L R D P S C V F P N R R K G A S F L P H T L A Y H K P V G E E P T Y S P A F N R S A M .......................................................................................................................... taaattccgtttgcagatgagtcgatccggagagcatacgaaaggatttcgtctcttgccggcagaaaaaagagggtgtgtcaaagcgtaatgtttgggcacgccctcttcaggtgtgtaagaaggggcaaaatttcgggacgctatggatgaaggttcgggagcatacggctagtatgcccccaaactttcatctcacgaacaatgagatgtttgaaaggttgtgacaccttgtctctcgatctggaaaaatatgagaaacaccgtta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaattccgtttgcagatgagtcgatccggagagcatacgaaaggatttcgtctcttgccggcagaaaaaagagggtgtgtcaaagcgtaatgtttgggcacgccctcttcaggtgtgtaagaaggggcaaaatttcgggacgctatggatgaaggttcgggagcatacggctagtatgcccccaaactttcatctcacgaacaatgagatgtttgaaaggttgtgacaccttgtctctcgatctggaaaaatatgagaaacaccgtta Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 83 HP_score: -5.0 Tail_Score: -5.24129 Start: 72 End: 108 Full_Region: tcttacacacctgaa gagggcgtgcccaaac attac gctttgacacaccctc ttttttctgccggca .......................................................................gagggcgtgcccaaacattacgctttgacacaccctc................................................................................................................................................................. TransTerm Strand: - Conf: 79 HP_score: -11.5 Tail_Score: -3.18582 Start: 155 End: 189 Full_Region: tgttcgtgagatgaa agtttgggggcatac tagcc gtatgctcccgaacc ttcatccatagcgtc ..........................................................................................................................................................agtttgggggcatactagccgtatgctcccgaacc................................................................................ TransTerm Strand: + Conf: 82 HP_score: -10.6 Tail_Score: -3.83026 Start: 156 End: 188 Full_Region: ACGCTATGGATGAAG GTTCGGGAGCATAC GGCTA GTATGCCCCCAAAC TTTCATCTCACGAAC ...........................................................................................................................................................GTTCGGGAGCATACGGCTAGTATGCCCCCAAAC................................................................................. TransTerm Strand: - Conf: 89 HP_score: -15.0 Tail_Score: -2.50935 Start: 156 End: 188 Full_Region: gttcgtgagatgaaa gtttgggggcatac tagcc gtatgctcccgaac cttcatccatagcgt ...........................................................................................................................................................gtttgggggcatactagccgtatgctcccgaac................................................................................. TransTerm Strand: + Conf: 56 HP_score: -5.5 Tail_Score: -3.58533 Start: 192 End: 212 Full_Region: ATGCCCCCAAACTTT CATCTCA CGAACAA TGAGATG TTTGAAAGGTTGTGA ...............................................................................................................................................................................................CATCTCACGAACAATGAGATG......................................................... Find igs database================================================ Query_seq: TF0178:TF0179|TF0178:TF0179:ferritin:pyrophosphate-energized proton pump:->->:188284..188552 269 taaattccgtttgcagatgagtcgatccggagagcatacgaaaggatttcgtctcttgccggcagaaaaaagagggtgtgtcaaagcgtaatgtttgggcacgccctcttcaggtgtgtaagaaggggcaaaatttcgggacgctatggatgaaggttcgggagcatacggctagtatgcccccaaactttcatctcacgaacaatgagatgtttgaaaggttgtgacaccttgtctctcgatctggaaaaatatgagaaacaccgtta Intra-Species Hit: Count: 1 Min: 1 Max: 269 Len: 269 Subject: tfor_TF0178_TF0179|ferritin:pyrophosphate-energized proton pump|POSITIVE:POSITIVE|[188284,188552]|269 HSP 1 e-value: 1.0E-150 bit: 533.0 Len: 269 Query Start:1 Query End:269 Subject Strand: POSITIVE Subject Start: 1 Subject End: 269 taaattccgtttgcagatgagtcgatccggagagcatacgaaaggatttcgtctcttgccggcagaaaaaagagggtgtgtcaaagcgtaatgtttgggcacgccctcttcaggtgtgtaagaaggggcaaaatttcgggacgctatggatgaaggttcgggagcatacggctagtatgcccccaaactttcatctcacgaacaatgagatgtttgaaaggttgtgacaccttgtctctcgatctggaaaaatatgagaaacaccgtta taaattccgtttgcagatgagtcgatccggagagcatacgaaaggatttcgtctcttgccggcagaaaaaagagggtgtgtcaaagcgtaatgtttgggcacgccctcttcaggtgtgtaagaaggggcaaaatttcgggacgctatggatgaaggttcgggagcatacggctagtatgcccccaaactttcatctcacgaacaatgagatgtttgaaaggttgtgacaccttgtctctcgatctggaaaaatatgagaaacaccgtta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAUUCCGUUUGCAGAUGAGUCGAUCCGGAGAGCAUACGAAAGGAUUUCGUCUCUUGCCGGCAGAAAAAAGAGGGUGUGUCAAAGCGUAAUGUUUGGGCACGCCCUCUUCAGGUGUGUAAGAAGGGGCAAAAUUUCGGGACGCUAUGGAUGAAGGUUCGGGAGCAUACGGCUAGUAUGCCCCCAAACUUUCAUCUCACGAACAAUGAGAUGUUUGAAAGGUUGUGACACCUUGUCUCUCGAUCUGGAAAAAUAUGAGAAACACCGUUA ....(((.((..((((..(((.((((((...............)))..))).)))))))..)).)))..(((((((((((((.((((.....)))).)))))))))))))..(((((....((.(((((((....(((.(((.((..(((((((((((.(((.((((((.....)))))).))).)))))))))))..((((((......))))))..))))).)))....)))))))))..(((.(.......).))).))))).... (-94.56) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAACGGUGUUUCUCAUAUUUUUCCAGAUCGAGAGACAAGGUGUCACAACCUUUCAAACAUCUCAUUGUUCGUGAGAUGAAAGUUUGGGGGCAUACUAGCCGUAUGCUCCCGAACCUUCAUCCAUAGCGUCCCGAAAUUUUGCCCCUUCUUACACACCUGAAGAGGGCGUGCCCAAACAUUACGCUUUGACACACCCUCUUUUUUCUGCCGGCAAGAGACGAAAUCCUUUCGUAUGCUCUCCGGAUCGACUCAUCUGCAAACGGAAUUUA ....((((..((...........((((..(((((.....((((((..........((((......)))).(((.((((((.((((((((((((((.....)))))))))))))).))))))))).((((..........(((((((((..........)))).)))))............))))..))))))...)))))...))))((((..((..(((((.....)))))...))..))))...))..))))............... (-83.15) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 825 120 71 172 120 tfor:TF0476|5end_conserved_hypothetical_protein_494944..495174_POSITIVE 762 120 72 181 132 tfor:TF2536|5end_subtilisin-like_serine_protease_2719286..2719516_POSITIVE 696 120 71 175 122 tfor:TF0792|5end_pyruvate_ferredoxin;_flavodoxin_oxidoreductase_846454..846684_POSITIVE 574 109 62 155 112 tfor:TF1284|5end_glyceraldehyde_3-phosphate_dehydrogenase_1367939..1368169_POSITIVE 565 110 64 227 183 tfor:TF0184|5end_dihydroneopterin_aldolase_195629..195859_POSITIVE 522 116 72 200 155 tfor:TF3007|5end_conserved_hypothetical_protein_3227256..3227486_POSITIVE 517 119 72 182 136 tfor:TF0146|5end_glutamine-dependent_NAD(+)_synthetase_154855..155085_POSITIVE 502 119 71 173 125 tfor:TF0702|5end_possible_peptidyl-prolyl_isomerase_744977..745207_POSITIVE 499 105 68 151 114 tfor:TF1024|5end_50S_ribosomal_protein_L19_1081948..1082178_POSITIVE 483 107 70 167 130 tfor:TF0216|5end_50S_ribosomal_protein_L20_242476..242706_POSITIVE 475 110 69 203 161 tfor:TF2593|5end_amino_acid_transport_protein_2762882..2763112_POSITIVE 473 119 71 221 162 tfor:TF2320|5end_hypothetical_protein_2476654..2476884_POSITIVE 470 118 71 173 124 tfor:TF0811|5end_conserved_hypothetical_protein;_posible_sugar_phosphate_isomerase_869787..870017_POSITIVE 460 110 61 228 174 tfor:TF1999|5end_ArgK_protein_with_ATPase_and_kinase_domains_2152769..2152999_POSITIVE 447 110 72 199 160 tfor:TF0662|5end_hypothetical_protein_708316..708546_POSITIVE 440 110 61 203 154 tfor:TF2289|5end_secretion_protein,_possible_HlyD_family_2442725..2442955_POSITIVE 419 120 71 226 174 tfor:TF1505|5end_conserved_hypothetical_protein_1610773..1611003_POSITIVE 416 64 21 52 2 tfor:TF2398|5end_magnesium_chelatase,_D/I_family_2569549..2569779_POSITIVE 413 118 71 84 37 tfor:TF1046|5end_hypothetical_protein_1108178..1108408_POSITIVE 411 65 21 217 175 tfor:TF2596|5end_conserved_hypothetical_protein;_possible_lipoprotein_2766667..2766897_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 565 110 64 46 2 tfor:TF0182|3end_DNA-binding_protein,_HU-related_195528..195678_POSITIVE 503 119 72 117 74 tfor:TF2413|3end_hypothetical_protein_2590680..2590830_POSITIVE 477 108 68 49 9 tfor:TF2765|3end_subtilisin-like_serine_protease_2951460..2951610_POSITIVE 475 110 69 48 6 tfor:TF2592|3end_conserved_hypothetical_protein_2762807..2762957_POSITIVE 473 119 71 75 16 tfor:TF2319|3end_acyl_carrier_protein_2476588..2476738_POSITIVE 460 110 61 65 11 tfor:TF1997|3end_ROK_family_transcriptional_regulator_2152686..2152836_POSITIVE 447 110 72 60 21 tfor:TF0661|3end_hypothetical_protein_708257..708407_POSITIVE 413 118 71 71 24 tfor:TF1045|3end_conserved_hypothetical_protein_1108245..1108395_POSITIVE 403 63 21 74 31 tfor:TF0525|3end_conserved_hypothetical_protein_549584..549734_POSITIVE 397 109 71 60 22 tfor:TF1354|3end_TonB_protein_1435216..1435366_POSITIVE 393 116 71 80 23 tfor:TF0105|3end_DNA-binding_stress_protein,_Dps_family_113907..114057_POSITIVE 385 110 64 56 11 tfor:TF1416|3end_outer_membrane_protein_1499413..1499563_POSITIVE 368 119 71 49 1 tfor:TF3144|3end_phosphomannomutase/phosphoglucomutase_3381184..3381334_POSITIVE 360 116 71 54 1 tfor:TF2922|3end_surface_antigen_BspA_3121538..3121688_POSITIVE 354 172 137 147 106 tfor:TF0994|3end_maltose_O-acetyltransferase_1049782..1049932_POSITIVE 352 109 71 110 67 tfor:TF1452|3end_conserved_hypothetical_protein;_possible_transglutaminase-like_enzyme_1539657..1539807_POSITIVE 348 100 51 96 57 tfor:TF1740|3end_hypothetical_protein_1869192..1869342_POSITIVE 348 165 125 70 31 tfor:TF0752|3end_nucleotide-binding_protein;_MAF-like_protein_801713..801863_POSITIVE 347 65 26 148 112 tfor:TF0217|3end_50S_ribosomal_protein_L35_243194..243344_POSITIVE 344 167 122 130 76 tfor:TF2876|3end_cytosine_deaminase_3065664..3065814_POSITIVE