Origin IGS: ggcggcttcggttgcaattgctgctgtctctcattggttattatttaatcggcggatcgtttatgtgccttttagtatcctccggtatgcttgtttactttattcagatgcatccaaataagtctttgaataataaaataatagaaattgttagtgcatttagtatggagatatatctttcgcatatggcaatttttcaactaataaagaaaatacatattaacgaactactcggaaatggatggatccaatacataatgactgtttgggtggttttgtgcggctcgatgatattttcaatgattgtgagatgggtttttacacaggttaaaaataaaatgcatcaataaagaggtttgttcggaagatgtttttatatatttgataattaaatcaataaaaaattattcatctgcggcagtaatacgtgcggcttacaaaaggatcggttttattgtttttttaacatgtttgttgcgatatattccctacctttgggctactttgatctcttaaataaaatattcacccaatttaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ttaaattgggtgaatattttatttaagagatcaaagtagcccaaaggtagggaatatatcgcaacaaacatgttaaaaaaacaataaaaccgatccttttgtaagccgcacgtattactgccgcagatgaataattttttattgatttaattatcaaatatataaaaacatcttccgaacaaacctctttattgatgcattttatttttaacctgtgtaaaaacccatctcacaatcattgaaaatatcatcgagccgcacaaaaccacccaaacagtcattatgtattggatccatccatttccgagtagttcgttaatatgtattttctttattagttgaaaaattgccatatgcgaaagatatatctccatactaaatgcactaacaatttctattattttattattcaaagacttatttggatgcatctgaataaagtaaacaagcataccggaggatactaaaaggcacataaacgatccgccgattaaataataaccaatgagagacagcagcaattgcaaccgaagccgcc Mask Tandem Repeat Region ================================================ ggcggcttcggttgcaattgctgctgtctctcattggttattatttaatcggcggatcgtttatgtgccttttagtatcctccggtatgcttgtttactttattcagatgcatccaaataagtctttgaataataaaataatagaaattgttagtgcatttagtatggagatatatctttcgcatatggcaatttttcaactaataaagaaaatacatattaacgaactactcggaaatggatggatccaatacataatgactgtttgggtggttttgtgcggctcgatgatattttcaatgattgtgagatgggtttttacacaggttaaaaataaaatgcatcaataaagaggtttgttcggaagatgtttttatatatttgataattaaatcaataaaaaattattcatctgcggcagtaatacgtgcggcttacaaaaggatcggttttattgtttttttaacatgtttgttgcgatatattccctacctttgggctactttgatctcttaaataaaatattcacccaatttaa Find is-nt database================================================ Query_seq: TF0177:TF0178|TF0177:TF0178:hypothetical protein:ferritin:->->:187215..187752 538 ggcggcttcggttgcaattgctgctgtctctcattggttattatttaatcggcggatcgtttatgtgccttttagtatcctccggtatgcttgtttactttattcagatgcatccaaataagtctttgaataataaaataatagaaattgttagtgcatttagtatggagatatatctttcgcatatggcaatttttcaactaataaagaaaatacatattaacgaactactcggaaatggatggatccaatacataatgactgtttgggtggttttgtgcggctcgatgatattttcaatgattgtgagatgggtttttacacaggttaaaaataaaatgcatcaataaagaggtttgttcggaagatgtttttatatatttgataattaaatcaataaaaaattattcatctgcggcagtaatacgtgcggcttacaaaaggatcggttttattgtttttttaacatgtttgttgcgatatattccctacctttgggctactttgatctcttaaataaaatattcacccaatttaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF0177:TF0178|TF0177:TF0178:hypothetical protein:ferritin:->->:187215..187752 538 ggcggcttcggttgcaattgctgctgtctctcattggttattatttaatcggcggatcgtttatgtgccttttagtatcctccggtatgcttgtttactttattcagatgcatccaaataagtctttgaataataaaataatagaaattgttagtgcatttagtatggagatatatctttcgcatatggcaatttttcaactaataaagaaaatacatattaacgaactactcggaaatggatggatccaatacataatgactgtttgggtggttttgtgcggctcgatgatattttcaatgattgtgagatgggtttttacacaggttaaaaataaaatgcatcaataaagaggtttgttcggaagatgtttttatatatttgataattaaatcaataaaaaattattcatctgcggcagtaatacgtgcggcttacaaaaggatcggttttattgtttttttaacatgtttgttgcgatatattccctacctttgggctactttgatctcttaaataaaatattcacccaatttaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF0177:TF0178|TF0177:TF0178:hypothetical protein:ferritin:->->:187215..187752 538 ggcggcttcggttgcaattgctgctgtctctcattggttattatttaatcggcggatcgtttatgtgccttttagtatcctccggtatgcttgtttactttattcagatgcatccaaataagtctttgaataataaaataatagaaattgttagtgcatttagtatggagatatatctttcgcatatggcaatttttcaactaataaagaaaatacatattaacgaactactcggaaatggatggatccaatacataatgactgtttgggtggttttgtgcggctcgatgatattttcaatgattgtgagatgggtttttacacaggttaaaaataaaatgcatcaataaagaggtttgttcggaagatgtttttatatatttgataattaaatcaataaaaaattattcatctgcggcagtaatacgtgcggcttacaaaaggatcggttttattgtttttttaacatgtttgttgcgatatattccctacctttgggctactttgatctcttaaataaaatattcacccaatttaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ggcggcttcggttgcaattgctgctgtctctcattggttattatttaatcggcggatcgtttatgtgccttttagtatcctccggtatgcttgtttactttattcagatgcatccaaataagtctttgaataataaaataatagaaattgttagtgcatttagtatggagatatatctttcgcatatggcaatttttcaactaataaagaaaatacatattaacgaactactcggaaatggatggatccaatacataatgactgtttgggtggttttgtgcggctcgatgatattttcaatgattgtgagatgggtttttacacaggttaaaaataaaatgcatcaataaagaggtttgttcggaagatgtttttatatatttgataattaaatcaataaaaaattattcatctgcggcagtaatacgtgcggcttacaaaaggatcggttttattgtttttttaacatgtttgttgcgatatattccctacctttgggctactttgatctcttaaataaaatattcacccaatttaa Predict ORF larger than 30AA ================================================ Protein_Len: 34 Strand: + Start: 17 End: 118 ................ M A A V S H W L L F N R R I V Y V P F S I L R Y A C L L Y S D A S K .................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: + Start: 12 End: 347 ........... M Q L L L S L I G Y Y L I G G S F M C L L V S S G M L V Y F I Q M H P N K S L N N K I I E I V S A F S M E I Y L S H M A ............................................................................................................................................................................................... Protein_Len: 60 Strand: + Start: 334 End: 513 ............................................................................................................................................................................................................................................................................................................................................. M K C I N K E V C S E D V F I Y L I I K S I K N Y S S A A V I R A A Y K R I G F I V F L T C L L R Y I P Y L W A T L I S ......................... Protein_Len: 48 Strand: + Start: 393 End: 536 ........................................................................................................................................................................................................................................................................................................................................................................................................ M N K K L F I C G S N T C G L Q K D R F Y C F F N M F V A I Y S L P L G Y F D L L N K I F T Q F .. Protein_Len: 33 Strand: - Start: 300 End: 398 ........................................................................................................................................................................................................................................................................................................... H N H S P N K C L N F I F H M L L S T Q E S S T K I Y K I I L D M ............................................................................................................................................ Protein_Len: 30 Strand: - Start: 223 End: 312 .............................................................................................................................................................................................................................. R V V R F H I S G I C L S Q K P P K T R S S S I K L S Q S M .................................................................................................................................................................................................................................. Protein_Len: 47 Strand: - Start: 200 End: 340 ....................................................................................................................................................................................................... S I F F I C I L S S S P F P H I W Y M I V T Q T T K H P E I I N E I I T L H T K V C T L F L M ...................................................................................................................................................................................................... Protein_Len: 42 Strand: - Start: 75 End: 200 .......................................................................... Y G G T H K N V K N L H M W I L R Q I I F Y Y F N N T C K T H L Y I K R M H C N K M .................................................................................................................................................................................................................................................................................................................................................. ggcggcttcggttgcaattgctgctgtctctcattggttattatttaatcggcggatcgtttatgtgccttttagtatcctccggtatgcttgtttactttattcagatgcatccaaataagtctttgaataataaaataatagaaattgttagtgcatttagtatggagatatatctttcgcatatggcaatttttcaactaataaagaaaatacatattaacgaactactcggaaatggatggatccaatacataatgactgtttgggtggttttgtgcggctcgatgatattttcaatgattgtgagatgggtttttacacaggttaaaaataaaatgcatcaataaagaggtttgttcggaagatgtttttatatatttgataattaaatcaataaaaaattattcatctgcggcagtaatacgtgcggcttacaaaaggatcggttttattgtttttttaacatgtttgttgcgatatattccctacctttgggctactttgatctcttaaataaaatattcacccaatttaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ggcggcttcggttgcaattgctgctgtctctcattggttattatttaatcggcggatcgtttatgtgccttttagtatcctccggtatgcttgtttactttattcagatgcatccaaataagtctttgaataataaaataatagaaattgttagtgcatttagtatggagatatatctttcgcatatggcaatttttcaactaataaagaaaatacatattaacgaactactcggaaatggatggatccaatacataatgactgtttgggtggttttgtgcggctcgatgatattttcaatgattgtgagatgggtttttacacaggttaaaaataaaatgcatcaataaagaggtttgttcggaagatgtttttatatatttgataattaaatcaataaaaaattattcatctgcggcagtaatacgtgcggcttacaaaaggatcggttttattgtttttttaacatgtttgttgcgatatattccctacctttgggctactttgatctcttaaataaaatattcacccaatttaa Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF0177:TF0178|TF0177:TF0178:hypothetical protein:ferritin:->->:187215..187752 538 ggcggcttcggttgcaattgctgctgtctctcattggttattatttaatcggcggatcgtttatgtgccttttagtatcctccggtatgcttgtttactttattcagatgcatccaaataagtctttgaataataaaataatagaaattgttagtgcatttagtatggagatatatctttcgcatatggcaatttttcaactaataaagaaaatacatattaacgaactactcggaaatggatggatccaatacataatgactgtttgggtggttttgtgcggctcgatgatattttcaatgattgtgagatgggtttttacacaggttaaaaataaaatgcatcaataaagaggtttgttcggaagatgtttttatatatttgataattaaatcaataaaaaattattcatctgcggcagtaatacgtgcggcttacaaaaggatcggttttattgtttttttaacatgtttgttgcgatatattccctacctttgggctactttgatctcttaaataaaatattcacccaatttaa Intra-Species Hit: Count: 1 Min: 1 Max: 538 Len: 538 Subject: tfor_TF0177_TF0178|hypothetical protein:ferritin|POSITIVE:POSITIVE|[187215,187752]|538 HSP 1 e-value: 0.0 bit: 1025.0 Len: 538 Query Start:1 Query End:538 Subject Strand: POSITIVE Subject Start: 1 Subject End: 538 ggcggcttcggttgcaattgctgctgtctctcattggttattatttaatcggcggatcgtttatgtgccttttagtatcctccggtatgcttgtttactttattcagatgcatccaaataagtctttgaataataaaataatagaaattgttagtgcatttagtatggagatatatctttcgcatatggcaatttttcaactaataaagaaaatacatattaacgaactactcggaaatggatggatccaatacataatgactgtttgggtggttttgtgcggctcgatgatattttcaatgattgtgagatgggtttttacacaggttaaaaataaaatgcatcaataaagaggtttgttcggaagatgtttttatatatttgataattaaatcaataaaaaattattcatctgcggcagtaatacgtgcggcttacaaaaggatcggttttattgnnnnnnnaacatgtttgttgcgatatattccctacctttgggctactttgatctcttaaataaaatattcacccaatttaa ggcggcttcggttgcaattgctgctgtctctcattggttattatttaatcggcggatcgtttatgtgccttttagtatcctccggtatgcttgtttactttattcagatgcatccaaataagtctttgaataataaaataatagaaattgttagtgcatttagtatggagatatatctttcgcatatggcaatttttcaactaataaagaaaatacatattaacgaactactcggaaatggatggatccaatacataatgactgtttgggtggttttgtgcggctcgatgatattttcaatgattgtgagatgggtttttacacaggttaaaaataaaatgcatcaataaagaggtttgttcggaagatgtttttatatatttgataattaaatcaataaaaaattattcatctgcggcagtaatacgtgcggcttacaaaaggatcggttttattgtttttttaacatgtttgttgcgatatattccctacctttgggctactttgatctcttaaataaaatattcacccaatttaa Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GGCGGCUUCGGUUGCAAUUGCUGCUGUCUCUCAUUGGUUAUUAUUUAAUCGGCGGAUCGUUUAUGUGCCUUUUAGUAUCCUCCGGUAUGCUUGUUUACUUUAUUCAGAUGCAUCCAAAUAAGUCUUUGAAUAAUAAAAUAAUAGAAAUUGUUAGUGCAUUUAGUAUGGAGAUAUAUCUUUCGCAUAUGGCAAUUUUUCAACUAAUAAAGAAAAUACAUAUUAACGAACUACUCGGAAAUGGAUGGAUCCAAUACAUAAUGACUGUUUGGGUGGUUUUGUGCGGCUCGAUGAUAUUUUCAAUGAUUGUGAGAUGGGUUUUUACACAGGUUAAAAAUAAAAUGCAUCAAUAAAGAGGUUUGUUCGGAAGAUGUUUUUAUAUAUUUGAUAAUUAAAUCAAUAAAAAAUUAUUCAUCUGCGGCAGUAAUACGUGCGGCUUACAAAAGGAUCGGUUUUAUUGUUUUUUUAACAUGUUUGUUGCGAUAUAUUCCCUACCUUUGGGCUACUUUGAUCUCUUAAAUAAAAUAUUCACCCAAUUUAA .(((((....))))).(((((((((((......((((((((((..((...((((.((.....)).))))......((((.(((((...((((.((((..(((((((((.((..........)).)))))))))....................((((((((....((((((((((((..(((((((.......(((((..........)))))...........((((((((((((..((((....))))...(.....)....)))))))))))))))))))...))).))))))))).....(((((((.....))))))).............))))))))...)))).))))....))))).))))........))..))))))))))...(((((....))))).....)))))))))))....(..(((((...((((...((...((((((......((((....))))))))))....))...)))))))))..)................................... (-111.60) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUAAAUUGGGUGAAUAUUUUAUUUAAGAGAUCAAAGUAGCCCAAAGGUAGGGAAUAUAUCGCAACAAACAUGUUAAAAAAACAAUAAAACCGAUCCUUUUGUAAGCCGCACGUAUUACUGCCGCAGAUGAAUAAUUUUUUAUUGAUUUAAUUAUCAAAUAUAUAAAAACAUCUUCCGAACAAACCUCUUUAUUGAUGCAUUUUAUUUUUAACCUGUGUAAAAACCCAUCUCACAAUCAUUGAAAAUAUCAUCGAGCCGCACAAAACCACCCAAACAGUCAUUAUGUAUUGGAUCCAUCCAUUUCCGAGUAGUUCGUUAAUAUGUAUUUUCUUUAUUAGUUGAAAAAUUGCCAUAUGCGAAAGAUAUAUCUCCAUACUAAAUGCACUAACAAUUUCUAUUAUUUUAUUAUUCAAAGACUUAUUUGGAUGCAUCUGAAUAAAGUAAACAAGCAUACCGGAGGAUACUAAAAGGCACAUAAACGAUCCGCCGAUUAAAUAAUAACCAAUGAGAGACAGCAGCAAUUGCAACCGAAGCCGCC .....((((((...(((((((((.....))).)))))))))))).(((.((..((((((((((....(((((((((...............(((..(((((((.((.(((........))).)).(((((.(((.((......)).))).))))).....)))))))..)))...(((((....(((....((((((.....(((((((((..((((.............))))....))))))))).))))))...............(((((((.......))).))))..............)))..))))))))))))))...........(((((....))))).....)))))....)))))..))........((((.........(((.(((((((((((((.....(((((((..((....))..))).))))............(((.((((...................)))).)))....))))))))..))))).)))..........))))......)))... (-89.44) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 369 38 1 220 180 tfor:TF2255|5end_transfer_region-related_protein,_TraH_2427504..2427734_POSITIVE 348 37 1 153 107 tfor:TF2475|5end_GTP_pyrophosphokinase,_(p)ppGpp_synthetase_2651925..2652155_POSITIVE 340 70 21 127 78 tfor:TF1514|5end_hypothetical_protein_1625370..1625600_POSITIVE 340 70 21 64 15 tfor:TF1515|5end_hypothetical_protein_1625307..1625537_POSITIVE 332 42 2 196 155 tfor:TF2798|5end_conserved_hypothetical_protein_2984023..2984253_POSITIVE 329 99 51 93 40 tfor:TF3123|5end_dTDP-4-dehydrorhamnose_3,5-epimerase_3357775..3358005_POSITIVE 327 94 51 57 14 tfor:TF1796|5end_ABC_transporter,_ATP-binding_protein_1936492..1936722_POSITIVE 327 27 1 88 55 tfor:TF1174|5end_ABC_transporter,_permease_component_1256584..1256814_POSITIVE 326 500 467 72 38 tfor:TF3152|5end_hypothetical_protein_3386690..3386920_POSITIVE 324 434 405 212 185 tfor:TF1179|5end_two-component_sensor_histidine_kinase_1266057..1266287_POSITIVE 324 287 250 167 122 tfor:TF0011|5end_conserved_hypothetical_protein_4685..4915_POSITIVE 321 288 241 172 123 tfor:TF1510|5end_outer_membrane_protein_1619551..1619781_POSITIVE 321 92 51 56 20 tfor:TF1215|5end_haloacid_dehalogenase-like_hydrolase_1302510..1302740_POSITIVE 319 299 266 59 29 tfor:TF1672|5end_ATP_synthase,_B_subunit_1786726..1786956_POSITIVE 315 455 411 58 9 tfor:TF0322|5end_possible_YngK_protein_348345..348575_POSITIVE 314 41 1 199 140 tfor:TF0416|5end_possible_ATP-binding_protein_433134..433364_POSITIVE 313 89 50 197 149 tfor:TF0813|5end_glycosyl_hydrolase,_secreted_870677..870907_POSITIVE 312 56 13 190 139 tfor:TF2563|5end_30S_ribosomal_protein_S17_2739333..2739563_POSITIVE 312 89 49 56 3 tfor:TF0496|5end_conserved_hypothetical_protein_522332..522562_POSITIVE 311 449 410 70 22 tfor:TF0371|5end_beta-mannosidase_precursor_386763..386993_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 345 93 51 130 72 tfor:TF0939|3end_ISPg1_transposase,_IS1106-related_transposase_981115..981265_POSITIVE 340 70 21 127 78 tfor:TF1513|3end_hypothetical_protein_1625450..1625600_POSITIVE 327 27 1 75 42 tfor:TF1173|3end_ABC_transporter,_permease_component_1256651..1256801_POSITIVE 318 70 30 84 48 tfor:TF2921|3end_hypothetical_protein_3118790..3118940_POSITIVE 318 70 30 84 48 tfor:TF2438|3end_hypothetical_protein_2616157..2616307_POSITIVE 317 30 1 147 112 tfor:TF2473|3end_poly(A)_polymerase/tRNA_nucleotidyltransferase_2652010..2652160_POSITIVE 316 93 51 130 70 tfor:TF0874|3end_transposase_926652..926802_POSITIVE 314 41 1 113 54 tfor:TF0414|3end_N-ethylammeline_chlorohydrolase_433128..433278_POSITIVE 309 41 8 116 78 tfor:TF0788|3end_protein-export_membrane_protein_841370..841520_POSITIVE 308 39 1 96 63 tfor:TF0177|3end_hypothetical_protein_187154..187304_POSITIVE 307 329 282 82 37 tfor:TF3042|3end_ferrous_iron_transport_protein_B_3271949..3272099_POSITIVE 307 449 416 54 22 tfor:TF0300|3end_hypothetical_protein_325541..325691_POSITIVE 305 90 50 51 8 tfor:TF1917|3end_hypothetical_protein_2070244..2070394_POSITIVE 303 449 414 81 37 tfor:TF2336|3end_conserved_hypothetical_protein_2498159..2498309_POSITIVE 299 270 223 92 43 tfor:TF0478|3end_conserved_hypothetical_protein_498627..498777_POSITIVE 298 92 64 33 1 tfor:TF2727|3end_outer_membrane_protein,_possibly_involved_in_nutrient_binding_2904534..2904684_POSITIVE 298 25 1 149 124 tfor:TF1943|3end_phosphoenolpyruvate_carboxykinase_2092465..2092615_POSITIVE 298 308 265 151 112 tfor:TF0757|3end_50S_ribosomal_protein_L25;_general_stress_protein_805293..805443_POSITIVE 297 85 50 47 17 tfor:TF2357|3end_collagenase_2523900..2524050_POSITIVE 297 69 25 58 17 tfor:TF2340|3end_hypothetical_protein_2500915..2501065_POSITIVE