Origin IGS: gcttcaaaggtagggtaaaattccgaacgtaccattatatttcatcccgatgtgtgtgactttcgtttaatatccaaatttacaaacttttttgtcgaagagtccgtgaaaggtaatacttttaagggctcttgtaggtgcttattcatcctaaggttgcagaatactccgaagttttgcagtccaaacgaactatccggttttcgatgtaatagagatgaagcgtacttccctgttttacacatttccgtacgaaaagaggtgtctaatgtacgattttaccccctttgcattcttttcggctcctatatttgtcccgcaaatcatgatataatttaagaacacatctaataaaataataagagaagatacttc .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 gaagtatcttctcttattattttattagatgtgttcttaaattatatcatgatttgcgggacaaatataggagccgaaaagaatgcaaagggggtaaaatcgtacattagacacctcttttcgtacggaaatgtgtaaaacagggaagtacgcttcatctctattacatcgaaaaccggatagttcgtttggactgcaaaacttcggagtattctgcaaccttaggatgaataagcacctacaagagcccttaaaagtattacctttcacggactcttcgacaaaaaagtttgtaaatttggatattaaacgaaagtcacacacatcgggatgaaatataatggtacgttcggaattttaccctacctttgaagc Mask Tandem Repeat Region ================================================ gcttcaaaggtagggtaaaattccgaacgtaccattatatttcatcccgatgtgtgtgactttcgtttaatatccaaatttacaaacttttttgtcgaagagtccgtgaaaggtaatacttttaagggctcttgtaggtgcttattcatcctaaggttgcagaatactccgaagttttgcagtccaaacgaactatccggttttcgatgtaatagagatgaagcgtacttccctgttttacacatttccgtacgaaaagaggtgtctaatgtacgattttaccccctttgcattcttttcggctcctatatttgtcccgcaaatcatgatataatttaagaacacatctaataaaataataagagaagatacttc Find is-nt database================================================ Query_seq: TF0109:TF0110|TF0109:TF0110:diacylglycerol kinase:unsaturated glucuronyl hydrolase:->->:116597..116971 375 gcttcaaaggtagggtaaaattccgaacgtaccattatatttcatcccgatgtgtgtgactttcgtttaatatccaaatttacaaacttttttgtcgaagagtccgtgaaaggtaatacttttaagggctcttgtaggtgcttattcatcctaaggttgcagaatactccgaagttttgcagtccaaacgaactatccggttttcgatgtaatagagatgaagcgtacttccctgttttacacatttccgtacgaaaagaggtgtctaatgtacgattttaccccctttgcattcttttcggctcctatatttgtcccgcaaatcatgatataatttaagaacacatctaataaaataataagagaagatacttc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF0109:TF0110|TF0109:TF0110:diacylglycerol kinase:unsaturated glucuronyl hydrolase:->->:116597..116971 375 gcttcaaaggtagggtaaaattccgaacgtaccattatatttcatcccgatgtgtgtgactttcgtttaatatccaaatttacaaacttttttgtcgaagagtccgtgaaaggtaatacttttaagggctcttgtaggtgcttattcatcctaaggttgcagaatactccgaagttttgcagtccaaacgaactatccggttttcgatgtaatagagatgaagcgtacttccctgttttacacatttccgtacgaaaagaggtgtctaatgtacgattttaccccctttgcattcttttcggctcctatatttgtcccgcaaatcatgatataatttaagaacacatctaataaaataataagagaagatacttc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF0109:TF0110|TF0109:TF0110:diacylglycerol kinase:unsaturated glucuronyl hydrolase:->->:116597..116971 375 gcttcaaaggtagggtaaaattccgaacgtaccattatatttcatcccgatgtgtgtgactttcgtttaatatccaaatttacaaacttttttgtcgaagagtccgtgaaaggtaatacttttaagggctcttgtaggtgcttattcatcctaaggttgcagaatactccgaagttttgcagtccaaacgaactatccggttttcgatgtaatagagatgaagcgtacttccctgttttacacatttccgtacgaaaagaggtgtctaatgtacgattttaccccctttgcattcttttcggctcctatatttgtcccgcaaatcatgatataatttaagaacacatctaataaaataataagagaagatacttc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 gcttcaaaggtagggtaaaattccgaacgtaccattatatttcatcccgatgtgtgtgactttcgtttaatatccaaatttacaaacttttttgtcgaagagtccgtgaaaggtaatacttttaagggctcttgtaggtgcttattcatcctaaggttgcagaatactccgaagttttgcagtccaaacgaactatccggttttcgatgtaatagagatgaagcgtacttccctgttttacacatttccgtacgaaaagaggtgtctaatgtacgattttaccccctttgcattcttttcggctcctatatttgtcccgcaaatcatgatataatttaagaacacatctaataaaataataagagaagatacttc Predict ORF larger than 30AA ================================================ Protein_Len: 56 Strand: + Start: 164 End: 331 ................................................................................................................................................................... M L R S F A V Q T N Y P V F D V I E M K R T S L F Y T F P Y E K R C L M Y D F T P F A F F S A P I F V P Q I M I ............................................ Protein_Len: 60 Strand: + Start: 34 End: 336 ................................. M I F H P D V C D F R L I S K F T N F F V E E S V K G N T F K G S C R C L F I L R L Q N T P K F C S P N E L S G F R C N ....................................... Protein_Len: 34 Strand: - Start: 269 End: 370 ............................................................................................................................................................................................................................................................................ H V I K G G K C E K R S R Y K D R L D H Y L K L V C R I F Y Y S F M ..... Protein_Len: 40 Strand: - Start: 241 End: 360 ................................................................................................................................................................................................................................................ V N G Y S F L H R I Y S K V G K A N K E A G I N T G C I M I Y N L F V D L L I M ............... Protein_Len: 60 Strand: - Start: 45 End: 245 ............................................ K I D L N V F K K T S S D T F P L V K L P E Q L H K N M R L N C F V G F N Q L G F S S D P K R H L L S S A Y K G T K C M .................................................................................................................................. gcttcaaaggtagggtaaaattccgaacgtaccattatatttcatcccgatgtgtgtgactttcgtttaatatccaaatttacaaacttttttgtcgaagagtccgtgaaaggtaatacttttaagggctcttgtaggtgcttattcatcctaaggttgcagaatactccgaagttttgcagtccaaacgaactatccggttttcgatgtaatagagatgaagcgtacttccctgttttacacatttccgtacgaaaagaggtgtctaatgtacgattttaccccctttgcattcttttcggctcctatatttgtcccgcaaatcatgatataatttaagaacacatctaataaaataataagagaagatacttc Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ gcttcaaaggtagggtaaaattccgaacgtaccattatatttcatcccgatgtgtgtgactttcgtttaatatccaaatttacaaacttttttgtcgaagagtccgtgaaaggtaatacttttaagggctcttgtaggtgcttattcatcctaaggttgcagaatactccgaagttttgcagtccaaacgaactatccggttttcgatgtaatagagatgaagcgtacttccctgttttacacatttccgtacgaaaagaggtgtctaatgtacgattttaccccctttgcattcttttcggctcctatatttgtcccgcaaatcatgatataatttaagaacacatctaataaaataataagagaagatacttc Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 60 HP_score: -5.9 Tail_Score: -3.4071 Start: 100 End: 131 Full_Region: AACTTTTTTGTCGAA GAGTCCGTGAAAG GTAATA CTTTTAAGGGCTC TTGTAGGTGCTTATT ...................................................................................................GAGTCCGTGAAAGGTAATACTTTTAAGGGCTC.................................................................................................................................................................................................................................................... Find igs database================================================ Query_seq: TF0109:TF0110|TF0109:TF0110:diacylglycerol kinase:unsaturated glucuronyl hydrolase:->->:116597..116971 375 gcttcaaaggtagggtaaaattccgaacgtaccattatatttcatcccgatgtgtgtgactttcgtttaatatccaaatttacaaacttttttgtcgaagagtccgtgaaaggtaatacttttaagggctcttgtaggtgcttattcatcctaaggttgcagaatactccgaagttttgcagtccaaacgaactatccggttttcgatgtaatagagatgaagcgtacttccctgttttacacatttccgtacgaaaagaggtgtctaatgtacgattttaccccctttgcattcttttcggctcctatatttgtcccgcaaatcatgatataatttaagaacacatctaataaaataataagagaagatacttc Intra-Species Hit: Count: 1 Min: 1 Max: 375 Len: 375 Subject: tfor_TF0109_TF0110|diacylglycerol kinase:unsaturated glucuronyl hydrolase|POSITIVE:POSITIVE|[116597,116971]|375 HSP 1 e-value: 0.0 bit: 743.0 Len: 375 Query Start:1 Query End:375 Subject Strand: POSITIVE Subject Start: 1 Subject End: 375 gcttcaaaggtagggtaaaattccgaacgtaccattatatttcatcccgatgtgtgtgactttcgtttaatatccaaatttacaaacttttttgtcgaagagtccgtgaaaggtaatacttttaagggctcttgtaggtgcttattcatcctaaggttgcagaatactccgaagttttgcagtccaaacgaactatccggttttcgatgtaatagagatgaagcgtacttccctgttttacacatttccgtacgaaaagaggtgtctaatgtacgattttaccccctttgcattcttttcggctcctatatttgtcccgcaaatcatgatataatttaagaacacatctaataaaataataagagaagatacttc gcttcaaaggtagggtaaaattccgaacgtaccattatatttcatcccgatgtgtgtgactttcgtttaatatccaaatttacaaacttttttgtcgaagagtccgtgaaaggtaatacttttaagggctcttgtaggtgcttattcatcctaaggttgcagaatactccgaagttttgcagtccaaacgaactatccggttttcgatgtaatagagatgaagcgtacttccctgttttacacatttccgtacgaaaagaggtgtctaatgtacgattttaccccctttgcattcttttcggctcctatatttgtcccgcaaatcatgatataatttaagaacacatctaataaaataataagagaagatacttc Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GCUUCAAAGGUAGGGUAAAAUUCCGAACGUACCAUUAUAUUUCAUCCCGAUGUGUGUGACUUUCGUUUAAUAUCCAAAUUUACAAACUUUUUUGUCGAAGAGUCCGUGAAAGGUAAUACUUUUAAGGGCUCUUGUAGGUGCUUAUUCAUCCUAAGGUUGCAGAAUACUCCGAAGUUUUGCAGUCCAAACGAACUAUCCGGUUUUCGAUGUAAUAGAGAUGAAGCGUACUUCCCUGUUUUACACAUUUCCGUACGAAAAGAGGUGUCUAAUGUACGAUUUUACCCCCUUUGCAUUCUUUUCGGCUCCUAUAUUUGUCCCGCAAAUCAUGAUAUAAUUUAAGAACACAUCUAAUAAAAUAAUAAGAGAAGAUACUUC ....((((((..(((((((((......(((((.((((((((((.....((((((((.(((.....................(((((....)))))..((((((((.((((((......)))))).))))))))..((((((...(((((((((.(((((((((((........))))))))).))...((((((....)))..)))......))).))))))..))))))....))).))))))))((.....))...)))))))..)))))))))))))))))))))))...(((((((......((((((((.....))))).)))..........(((.....))).............)))))))...... (-86.30) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GAAGUAUCUUCUCUUAUUAUUUUAUUAGAUGUGUUCUUAAAUUAUAUCAUGAUUUGCGGGACAAAUAUAGGAGCCGAAAAGAAUGCAAAGGGGGUAAAAUCGUACAUUAGACACCUCUUUUCGUACGGAAAUGUGUAAAACAGGGAAGUACGCUUCAUCUCUAUUACAUCGAAAACCGGAUAGUUCGUUUGGACUGCAAAACUUCGGAGUAUUCUGCAACCUUAGGAUGAAUAAGCACCUACAAGAGCCCUUAAAAGUAUUACCUUUCACGGACUCUUCGACAAAAAAGUUUGUAAAUUUGGAUAUUAAACGAAAGUCACACACAUCGGGAUGAAAUAUAAUGGUACGUUCGGAAUUUUACCCUACCUUUGAAGC ......((..(((((((..............((((((((((((((...)))))))).))))))...)))))))..))........((((((((((((((((((((((((............(((((.....(((.....))).....))))).((((((((......(((.(((..((((.(((((((..(((..(.((.((((..(((..((((.......((((.((......))))))..))))..)))..)))).)).)..)))))))))).))))(((((....)))))...))).))).....((....))..........))))))))...)))).)))))......)))))))))..)))))).... (-79.13) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 324 217 178 43 1 tfor:TF2693|5end_possible_aminotransferase_2868435..2868665_POSITIVE 317 307 262 68 26 tfor:TF1915|5end_NAD-dependent_protein_deacetylase,_SIR2_family_2067720..2067950_POSITIVE 315 303 261 208 163 tfor:TF2604|5end_possible_membrane_transport_protein_2777407..2777637_POSITIVE 314 319 271 77 19 tfor:TF1013|5end_glycerol-3-phosphate_dehydrogenase_(NAD(P)(+))_(NAD(P)H-dependent_dihydroxyacetone-phosphate_reductase)_1070011..1070241_POSITIVE 313 231 194 216 178 tfor:TF2263|5end_transfer_region-related_protein,_TraN_2431901..2432131_POSITIVE 305 234 193 163 116 tfor:TF0888|5end_transfer_region-related_protein,_TraN_938392..938622_POSITIVE 300 319 273 71 26 tfor:TF2159|5end_conserved_hypothetical_protein_2334446..2334676_POSITIVE 298 239 194 203 141 tfor:TF1041|5end_dihydroorotate_dehydrogenase,_catalytic_subunit_1102029..1102259_POSITIVE 295 109 62 70 11 tfor:TF0968|5end_hypothetical_protein_1018908..1019138_POSITIVE 294 226 195 86 51 tfor:TF3146|5end_GTP-binding_protein_Obg_3381852..3382082_POSITIVE 294 306 261 60 18 tfor:TF2533|5end_adenylosuccinate_synthetase_2715666..2715896_POSITIVE 294 254 217 43 2 tfor:TF2196|5end_hypothetical_protein_2371678..2371908_POSITIVE 292 328 283 68 12 tfor:TF2317|5end_3-oxoacyl-[acyl-carrier-protein]_synthase_II_2474418..2474648_POSITIVE 291 185 156 215 170 tfor:TF2674|5end_conserved_hypothetical_protein;_possible_phosphoesterase_2848176..2848406_POSITIVE 290 322 282 217 179 tfor:TF0180|5end_carboxynorspermidine_decarboxylase_191267..191497_POSITIVE 288 267 223 148 99 tfor:TF1549|5end_conserved_hypothetical_protein_1653993..1654223_POSITIVE 287 237 193 68 24 tfor:TF1502|5end_exo-poly-alpha-D-galacturonosidase_precursor_1602796..1603026_POSITIVE 286 138 92 65 21 tfor:TF1342|5end_possible_lipoprotein_1423125..1423355_POSITIVE 286 291 254 186 143 tfor:TF1106|5end_conserved_hypothetical_protein_1174166..1174396_POSITIVE 284 265 222 53 11 tfor:TF0236|5end_multidrug_resistance_protein_261779..262009_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 329 57 14 85 41 tfor:TF1730|3end_diaminopimelate_decarboxylase_1855907..1856057_POSITIVE 300 319 273 129 84 tfor:TF2158|3end_conserved_hypothetical_protein_2334584..2334734_POSITIVE 297 325 282 47 3 tfor:TF0073|3end_possible_dTDP-glucose_4,6-dehydratase,_NAD-dependent_epimerase/dehydratase_family_82051..82201_POSITIVE 292 328 283 68 12 tfor:TF2315|3end_thioesterase_2474498..2474648_POSITIVE 289 235 196 147 105 tfor:TF0669|3end_hypothetical_protein_714051..714201_POSITIVE 288 267 223 62 13 tfor:TF1548|3end_thiophene_and_furan_oxidation_protein_1653987..1654137_POSITIVE 287 237 193 78 34 tfor:TF1501|3end_sugar_hydrolase_1602886..1603036_POSITIVE 286 138 92 60 16 tfor:TF1341|3end_glycogen_synthase_1423200..1423350_POSITIVE 286 291 254 118 75 tfor:TF1105|3end_arylsulfatase_regulator;_Fe-S_oxidoreductase_1174178..1174328_POSITIVE 284 265 222 43 1 tfor:TF0235|3end_hypothetical_protein_261849..261999_POSITIVE 282 303 262 125 91 tfor:TF0975|3end_hypothetical_protein_1024361..1024511_POSITIVE 280 236 197 150 113 tfor:TF3001|3end_transposase_3217692..3217842_POSITIVE 276 309 269 141 86 tfor:TF2547|3end_hypothetical_protein_2730947..2731097_POSITIVE 276 141 101 128 97 tfor:TF2245|3end_conserved_hypothetical_protein_2421291..2421441_POSITIVE 274 199 155 84 41 tfor:TF2366|3end_conserved_hypothetical_protein_2531892..2532042_POSITIVE 273 226 189 140 103 tfor:TF2825|3end_transposase_3017669..3017819_POSITIVE 273 226 189 140 103 tfor:TF2591|3end_transposase_2758920..2759070_POSITIVE 273 170 121 115 67 tfor:TF1786|3end_hypothetical_protein_1926425..1926575_POSITIVE 273 226 189 140 103 tfor:TF0716|3end_transposase_767576..767726_POSITIVE 271 289 242 100 52 tfor:TF2839|3end_ATP-dependent_exodeoxyribonuclease_3031035..3031185_POSITIVE