Origin IGS: ctctacatggtccaaataactccggcgcaaagataaggataaaacaaaagaacacgtttatatatgcgctttgactgcttggctgcatggatagaatgaatgttgagcacttgtgtatttatgatgaatcaatttttattaataggatttta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taaaatcctattaataaaaattgattcatcataaatacacaagtgctcaacattcattctatccatgcagccaagcagtcaaagcgcatatataaacgtgttcttttgttttatccttatctttgcgccggagttatttggaccatgtagag Mask Tandem Repeat Region ================================================ ctctacatggtccaaataactccggcgcaaagataaggataaaacaaaagaacacgtttatatatgcgctttgactgcttggctgcatggatagaatgaatgttgagcacttgtgtatttatgatgaatcaatttttattaataggatttta Find is-nt database================================================ Query_seq: TF0108:TF0109|TF0108:TF0109:thiamine biosynthesis protein, ThiF family:diacylglycerol kinase:->->:116061..116212 152 ctctacatggtccaaataactccggcgcaaagataaggataaaacaaaagaacacgtttatatatgcgctttgactgcttggctgcatggatagaatgaatgttgagcacttgtgtatttatgatgaatcaatttttattaataggatttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF0108:TF0109|TF0108:TF0109:thiamine biosynthesis protein, ThiF family:diacylglycerol kinase:->->:116061..116212 152 ctctacatggtccaaataactccggcgcaaagataaggataaaacaaaagaacacgtttatatatgcgctttgactgcttggctgcatggatagaatgaatgttgagcacttgtgtatttatgatgaatcaatttttattaataggatttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF0108:TF0109|TF0108:TF0109:thiamine biosynthesis protein, ThiF family:diacylglycerol kinase:->->:116061..116212 152 ctctacatggtccaaataactccggcgcaaagataaggataaaacaaaagaacacgtttatatatgcgctttgactgcttggctgcatggatagaatgaatgttgagcacttgtgtatttatgatgaatcaatttttattaataggatttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ctctacatggtccaaataactccggcgcaaagataaggataaaacaaaagaacacgtttatatatgcgctttgactgcttggctgcatggatagaatgaatgttgagcacttgtgtatttatgatgaatcaatttttattaataggatttta Predict ORF larger than 30AA ================================================ Protein_Len: 41 Strand: - Start: 6 End: 125 ..... * M T W I V G A C L Y P Y F L L V R K Y I R K S Q K A A H I S H I N L V Q T N I M ........................... Protein_Len: 39 Strand: - Start: 2 End: 118 . R C P G F L E P A F I L I F C F F V N I Y A S Q S S P Q M S L I F T S C K H M .................................. ctctacatggtccaaataactccggcgcaaagataaggataaaacaaaagaacacgtttatatatgcgctttgactgcttggctgcatggatagaatgaatgttgagcacttgtgtatttatgatgaatcaatttttattaataggatttta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ctctacatggtccaaataactccggcgcaaagataaggataaaacaaaagaacacgtttatatatgcgctttgactgcttggctgcatggatagaatgaatgttgagcacttgtgtatttatgatgaatcaatttttattaataggatttta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF0108:TF0109|TF0108:TF0109:thiamine biosynthesis protein, ThiF family:diacylglycerol kinase:->->:116061..116212 152 ctctacatggtccaaataactccggcgcaaagataaggataaaacaaaagaacacgtttatatatgcgctttgactgcttggctgcatggatagaatgaatgttgagcacttgtgtatttatgatgaatcaatttttattaataggatttta Intra-Species Hit: Count: 1 Min: 1 Max: 152 Len: 152 Subject: tfor_TF0108_TF0109|thiamine biosynthesis protein, ThiF family:diacylglycerol kinase|POSITIVE:POSITIVE|[116061,116212]|152 HSP 1 e-value: 3.0E-81 bit: 301.0 Len: 152 Query Start:1 Query End:152 Subject Strand: POSITIVE Subject Start: 1 Subject End: 152 ctctacatggtccaaataactccggcgcaaagataaggataaaacaaaagaacacgtttatatatgcgctttgactgcttggctgcatggatagaatgaatgttgagcacttgtgtatttatgatgaatcaatttttattaataggatttta ctctacatggtccaaataactccggcgcaaagataaggataaaacaaaagaacacgtttatatatgcgctttgactgcttggctgcatggatagaatgaatgttgagcacttgtgtatttatgatgaatcaatttttattaataggatttta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CUCUACAUGGUCCAAAUAACUCCGGCGCAAAGAUAAGGAUAAAACAAAAGAACACGUUUAUAUAUGCGCUUUGACUGCUUGGCUGCAUGGAUAGAAUGAAUGUUGAGCACUUGUGUAUUUAUGAUGAAUCAAUUUUUAUUAAUAGGAUUUUA ........(((((..........((((((...(((((...................)))))...)))))).....((((..((..(((.......)))...))..))))............(((((((......)))))))...)))))... (-26.51) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAAUCCUAUUAAUAAAAAUUGAUUCAUCAUAAAUACACAAGUGCUCAACAUUCAUUCUAUCCAUGCAGCCAAGCAGUCAAAGCGCAUAUAUAAACGUGUUCUUUUGUUUUAUCCUUAUCUUUGCGCCGGAGUUAUUUGGACCAUGUAGAG .......((((..........(((....)))........(((((((((.................(((......)))......(((((.(((.(((((........)))))......)))...)))))..))).))))))......)))).. (-18.70) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 343 108 65 130 81 tfor:TF2333|5end_signal_peptidase_I_2492560..2492790_POSITIVE 332 108 66 231 194 tfor:TF2738|5end_30S_ribosomal_protein_S16_2919303..2919533_POSITIVE 322 107 67 144 98 tfor:TF1182|5end_hypothetical_protein_1268565..1268795_POSITIVE 317 90 48 162 114 tfor:TF2616|5end_outer_membrane_protein,_TonB_dependent_receptor_2791470..2791700_POSITIVE 316 110 61 72 20 tfor:TF1451|5end_conserved_hypothetical_protein_1535876..1536106_POSITIVE 302 108 67 58 10 tfor:TF2747|5end_heat_shock_protein,_HSP90_family_2928279..2928509_POSITIVE 298 107 68 34 1 tfor:TF1187|5end_hypothetical_protein_1269625..1269855_POSITIVE 297 98 66 37 3 tfor:TF1278|5end_hypothetical_protein_1364050..1364280_POSITIVE 294 95 55 34 1 tfor:TF0437|5end_conserved_hypothetical_protein_460923..461153_POSITIVE 293 106 67 78 33 tfor:TF2817|5end_hypothetical_protein_3007358..3007588_POSITIVE 292 109 64 46 6 tfor:TF0583|5end_possible_gliding_motility-related_protein_613189..613419_POSITIVE 289 110 65 115 52 tfor:TF2954|5end_outer_membrane_protein,_TonB_dependent_receptor_3161238..3161468_POSITIVE 289 110 62 182 131 tfor:TF1554|5end_UDP-glucose_dehydrogenase_1659623..1659853_POSITIVE 286 108 64 78 21 tfor:TF0851|5end_helicase_SNF2_family_899486..899716_POSITIVE 286 110 63 57 3 tfor:TF0438|5end_polyferredoxin_461369..461599_POSITIVE 285 109 66 106 56 tfor:TF1485|5end_conserved_hypothetical_protein_1581871..1582101_POSITIVE 283 90 57 219 182 tfor:TF2807|5end_possible_chloride_channel_protein_2996637..2996867_POSITIVE 279 109 67 66 18 tfor:TF0700|5end_possible_lipoprotein_742649..742879_POSITIVE 279 108 64 50 3 tfor:TF0656|5end_acetyltransferase,_possible_GNAT_family_702045..702275_POSITIVE 278 47 5 49 3 tfor:TF0231|5end_conserved_hypothetical_protein_257804..258034_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 378 109 68 73 23 tfor:TF1153|3end_pyruvate-formate_lyase-activating_enzyme_1235673..1235823_POSITIVE 343 108 65 103 54 tfor:TF2332|3end_signal_peptidase_I_2492613..2492763_POSITIVE 331 105 66 147 115 tfor:TF2737|3end_conserved_hypothetical_protein;_TPR-repeat-containing_protein_2919304..2919454_POSITIVE 322 107 67 144 98 tfor:TF1186|3end_hypothetical_protein_1269815..1269965_POSITIVE 279 109 67 82 34 tfor:TF0699|3end_pyridoxal_phosphate_biosynthetic_protein_742745..742895_POSITIVE 279 109 65 40 1 tfor:TF0582|3end_hemolysin-related_protein,_with_CBS_domains;_possible_gliding-motility_related_protein_613263..613413_POSITIVE 275 88 46 59 19 tfor:TF0285|3end_conserved_hypothetical_protein_315534..315684_POSITIVE 262 86 52 59 16 tfor:TF2563|3end_30S_ribosomal_protein_S17_2739679..2739829_POSITIVE 261 90 57 124 94 tfor:TF1954|3end_long-chain-fatty-acid--CoA_ligase_2106940..2107090_POSITIVE 260 102 65 49 2 tfor:TF1909|3end_cobalamin_(5`-phosphate)_synthase_2064075..2064225_POSITIVE 260 35 1 122 84 tfor:TF0660|3end_conserved_hypothetical_protein_707399..707549_POSITIVE 260 109 65 66 19 tfor:TF0593|3end_membrane-associated_phospholipid_phosphatase_627051..627201_POSITIVE 258 103 64 64 15 tfor:TF0090|3end_conserved_hypothetical_protein_97747..97897_POSITIVE 257 99 53 130 82 tfor:TF2990|3end_nicotinate-nucleotide_pyrophosphorylase_3206389..3206539_POSITIVE 256 109 66 97 41 tfor:TF3029|3end_membrane_peptidase_3253758..3253908_POSITIVE 256 118 73 70 36 tfor:TF1915|3end_NAD-dependent_protein_deacetylase,_SIR2_family_2068489..2068639_POSITIVE 256 99 67 136 103 tfor:TF0408|3end_possible_gliding_motility-related_lipoprotein_426324..426474_POSITIVE 254 96 55 42 6 tfor:TF1284|3end_glyceraldehyde_3-phosphate_dehydrogenase_1369026..1369176_POSITIVE 254 99 56 50 2 tfor:TF0020|3end_possible_glycoprotein_endopeptidase_16029..16179_POSITIVE 253 109 65 106 59 tfor:TF2503|3end_conserved_hypothetical_protein_2680111..2680261_POSITIVE