Origin IGS: tgaggcattagtatcttatgaagtaaacaggaagagtttcgattgatagaggtttgcttttgtagaacctgtgtgatgccttaaacgtatcaggaaaatagggaaatcgtagaaaaaaatgaaatcaggaggggagtttcgttcgtaatgacgaatacggatatcctcctcatcctgattttacgattgattttatcctttggactcgtgcaggccggattgttta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taaacaatccggcctgcacgagtccaaaggataaaatcaatcgtaaaatcaggatgaggaggatatccgtattcgtcattacgaacgaaactcccctcctgatttcatttttttctacgatttccctattttcctgatacgtttaaggcatcacacaggttctacaaaagcaaacctctatcaatcgaaactcttcctgtttacttcataagatactaatgcctca Mask Tandem Repeat Region ================================================ tgaggcattagtatcttatgaagtaaacaggaagagtttcgattgatagaggtttgcttttgtagaacctgtgtgatgccttaaacgtatcaggaaaatagggaaatcgtagaaaaaaatgaaatcaggaggggagtttcgttcgtaatgacgaatacggatatcctcctcatcctgattttacgattgattttatcctttggactcgtgcaggccggattgttta Find is-nt database================================================ Query_seq: TF0100:TF0103|TF0100:TF0103:conserved hypothetical protein:rubredoxin:->->:111764..111989 226 tgaggcattagtatcttatgaagtaaacaggaagagtttcgattgatagaggtttgcttttgtagaacctgtgtgatgccttaaacgtatcaggaaaatagggaaatcgtagaaaaaaatgaaatcaggaggggagtttcgttcgtaatgacgaatacggatatcctcctcatcctgattttacgattgattttatcctttggactcgtgcaggccggattgttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF0100:TF0103|TF0100:TF0103:conserved hypothetical protein:rubredoxin:->->:111764..111989 226 tgaggcattagtatcttatgaagtaaacaggaagagtttcgattgatagaggtttgcttttgtagaacctgtgtgatgccttaaacgtatcaggaaaatagggaaatcgtagaaaaaaatgaaatcaggaggggagtttcgttcgtaatgacgaatacggatatcctcctcatcctgattttacgattgattttatcctttggactcgtgcaggccggattgttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF0100:TF0103|TF0100:TF0103:conserved hypothetical protein:rubredoxin:->->:111764..111989 226 tgaggcattagtatcttatgaagtaaacaggaagagtttcgattgatagaggtttgcttttgtagaacctgtgtgatgccttaaacgtatcaggaaaatagggaaatcgtagaaaaaaatgaaatcaggaggggagtttcgttcgtaatgacgaatacggatatcctcctcatcctgattttacgattgattttatcctttggactcgtgcaggccggattgttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tgaggcattagtatcttatgaagtaaacaggaagagtttcgattgatagaggtttgcttttgtagaacctgtgtgatgccttaaacgtatcaggaaaatagggaaatcgtagaaaaaaatgaaatcaggaggggagtttcgttcgtaatgacgaatacggatatcctcctcatcctgattttacgattgattttatcctttggactcgtgcaggccggattgttta Predict ORF larger than 30AA ================================================ Protein_Len: 40 Strand: + Start: 106 End: 225 ......................................................................................................... M V E K N E I R R G V S F V M T N T D I L L I L I L R L I L S F G L V Q A G L F . Protein_Len: 33 Strand: - Start: 93 End: 191 ............................................................................................ S F L S I T S F F S I L L P T E N T I V F V S I R R M R I K R N M ................................... Protein_Len: 48 Strand: - Start: 19 End: 162 .................. S T F L F L K S Q Y L N A K T S G T H H R L R I L F I P F D Y F F I F D P P S N R E Y H R I R M ................................................................ tgaggcattagtatcttatgaagtaaacaggaagagtttcgattgatagaggtttgcttttgtagaacctgtgtgatgccttaaacgtatcaggaaaatagggaaatcgtagaaaaaaatgaaatcaggaggggagtttcgttcgtaatgacgaatacggatatcctcctcatcctgattttacgattgattttatcctttggactcgtgcaggccggattgttta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tgaggcattagtatcttatgaagtaaacaggaagagtttcgattgatagaggtttgcttttgtagaacctgtgtgatgccttaaacgtatcaggaaaatagggaaatcgtagaaaaaaatgaaatcaggaggggagtttcgttcgtaatgacgaatacggatatcctcctcatcctgattttacgattgattttatcctttggactcgtgcaggccggattgttta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF0100:TF0103|TF0100:TF0103:conserved hypothetical protein:rubredoxin:->->:111764..111989 226 tgaggcattagtatcttatgaagtaaacaggaagagtttcgattgatagaggtttgcttttgtagaacctgtgtgatgccttaaacgtatcaggaaaatagggaaatcgtagaaaaaaatgaaatcaggaggggagtttcgttcgtaatgacgaatacggatatcctcctcatcctgattttacgattgattttatcctttggactcgtgcaggccggattgttta Intra-Species Hit: Count: 1 Min: 1 Max: 226 Len: 226 Subject: tfor_TF0100_TF0103|conserved hypothetical protein:rubredoxin|POSITIVE:POSITIVE|[111764,111989]|226 HSP 1 e-value: 1.0E-112 bit: 406.0 Len: 226 Query Start:1 Query End:226 Subject Strand: POSITIVE Subject Start: 1 Subject End: 226 tgaggcattagtatcttatgaagtaaacaggaagagtttcgattgatagaggtttgcttttgtagaacctgtgtgatgccttaaacgtatcaggaaaatagggaaatcgtagnnnnnnntgaaatcaggaggggagtttcgttcgtaatgacgaatacggatatcctcctcatcctgattttacgattgattttatcctttggactcgtgcaggccggattgttta tgaggcattagtatcttatgaagtaaacaggaagagtttcgattgatagaggtttgcttttgtagaacctgtgtgatgccttaaacgtatcaggaaaatagggaaatcgtagaaaaaaatgaaatcaggaggggagtttcgttcgtaatgacgaatacggatatcctcctcatcctgattttacgattgattttatcctttggactcgtgcaggccggattgttta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGAGGCAUUAGUAUCUUAUGAAGUAAACAGGAAGAGUUUCGAUUGAUAGAGGUUUGCUUUUGUAGAACCUGUGUGAUGCCUUAAACGUAUCAGGAAAAUAGGGAAAUCGUAGAAAAAAAUGAAAUCAGGAGGGGAGUUUCGUUCGUAAUGACGAAUACGGAUAUCCUCCUCAUCCUGAUUUUACGAUUGAUUUUAUCCUUUGGACUCGUGCAGGCCGGAUUGUUUA ((((((((((...((....)).....(((((..(.....).....(((((((....)))))))....))))).))))))))))...((((.((......((((((((((((((..........(((((((((((((.(((((((((....))))..)))))....)))))).)))))))))))))))).......)))))....)).))))............... (-61.21) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAACAAUCCGGCCUGCACGAGUCCAAAGGAUAAAAUCAAUCGUAAAAUCAGGAUGAGGAGGAUAUCCGUAUUCGUCAUUACGAACGAAACUCCCCUCCUGAUUUCAUUUUUUUCUACGAUUUCCCUAUUUUCCUGAUACGUUUAAGGCAUCACACAGGUUCUACAAAAGCAAACCUCUAUCAAUCGAAACUCUUCCUGUUUACUUCAUAAGAUACUAAUGCCUCA ((((((.....((((..(((.(((...((((.......((((((((((((((((.(.((((......(((..((((....)))))))...))))).)))))))))..........))))))).........))))))).)))...)))).......(((((((.....))..)))))....................))))))....................... (-45.09) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 329 160 119 89 41 tfor:TF1460|5end_6-phosphogluconate_dehydrogenase_1549262..1549492_POSITIVE 324 80 34 74 16 tfor:TF1718|5end_conserved_hypothetical_protein_1840462..1840692_POSITIVE 322 94 51 69 30 tfor:TF2665|5end_hypothetical_protein_2841973..2842203_POSITIVE 320 166 124 216 178 tfor:TF2188|5end_conserved_hypothetical_protein_2362652..2362882_POSITIVE 317 170 125 121 64 tfor:TF2172|5end_hypothetical_protein_2345822..2346052_POSITIVE 317 170 125 115 58 tfor:TF2173|5end_hypothetical_protein_2345816..2346046_POSITIVE 317 170 125 115 58 tfor:TF2171|5end_hypothetical_protein_2345485..2345715_POSITIVE 317 170 125 115 58 tfor:TF2166|5end_hypothetical_protein_2342115..2342345_POSITIVE 315 220 173 205 155 tfor:TF2314|5end_conserved_hypothetical_protein_2473530..2473760_POSITIVE 314 218 174 129 90 tfor:TF2705|5end_conserved_hypothetical_protein;_possible_SufE_Fe/S-cluster-related_protein_2876850..2877080_POSITIVE 313 217 171 66 13 tfor:TF0304|5end_peptidyl-prolyl_cis-trans_isomerase_329567..329797_POSITIVE 310 172 131 213 159 tfor:TF1170|5end_conserved_hypothetical_protein_1251862..1252092_POSITIVE 310 172 131 201 147 tfor:TF1171|5end_ABC_transporter,_ATP-binding_protein_1251850..1252080_POSITIVE 309 170 122 218 166 tfor:TF2410|5end_possible_endo-1,4-beta-xylanase_2584000..2584230_POSITIVE 309 175 131 118 65 tfor:TF0346|5end_hypothetical_protein_367048..367278_POSITIVE 308 190 141 80 30 tfor:TF0780|5end_alpha-L-arabinofuranosidase,_precursor_A_831944..832174_POSITIVE 307 136 91 89 50 tfor:TF1568|5end_H+-transporting_ATP_synthase,_subunit_D_1676341..1676571_POSITIVE 307 169 124 138 101 tfor:TF0626|5end_hypothetical_protein_661945..662175_POSITIVE 307 169 127 184 134 tfor:TF0017|5end_hypothetical_protein_10504..10734_POSITIVE 305 219 183 149 102 tfor:TF2887|5end_phosphomannomutase/phosphoglucomutase_3076327..3076557_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 349 175 131 54 1 tfor:TF1588|3end_hypothetical_protein_1702560..1702710_POSITIVE 337 169 129 78 33 tfor:TF1815|3end_conserved_hypothetical_protein_1957442..1957592_POSITIVE 329 160 119 67 19 tfor:TF1459|3end_possible_protease_1549320..1549470_POSITIVE 314 218 174 120 81 tfor:TF2704|3end_ribonuclease_HII_2876921..2877071_POSITIVE 307 136 91 89 50 tfor:TF1567|3end_V-type_ATP_synthase,_subunit_I_1676421..1676571_POSITIVE 307 169 124 58 21 tfor:TF0625|3end_hypothetical_protein_661945..662095_POSITIVE 294 169 126 57 16 tfor:TF2429|3end_ABC_transporter,_ATP-binding/permease_component,_bacteriocin/lantibiotic_exporter_2612734..2612884_POSITIVE 293 219 197 84 61 tfor:TF2745|3end_hypothetical_protein_2927947..2928097_POSITIVE 291 171 131 69 10 tfor:TF1760|3end_guanosine-3',5'-bis(diphosphate)_3'-pyrophosphohydrolase_1899413..1899563_POSITIVE 291 220 171 136 83 tfor:TF0903|3end_conserved_hypothetical_protein_948515..948665_POSITIVE 289 170 127 56 14 tfor:TF2452|3end_outer_membrane_protein,_OmpH_family_2630415..2630565_POSITIVE 289 176 131 136 79 tfor:TF1168|3end_hypothetical_protein_1249092..1249242_POSITIVE 287 175 131 149 113 tfor:TF0261|3end_conserved_hypothetical_protein_292118..292268_POSITIVE 286 80 40 112 67 tfor:TF2816|3end_hypothetical_protein_3007184..3007334_POSITIVE 284 189 143 84 37 tfor:TF1897|3end_conserved_hypothetical_protein;_possible_aminopeptidase_2052107..2052257_POSITIVE 284 176 132 142 93 tfor:TF0252|3end_hypothetical_protein_283759..283909_POSITIVE 283 114 71 95 45 tfor:TF2368|3end_uridine_phosphorylase_2534618..2534768_POSITIVE 282 179 143 51 18 tfor:TF3043|3end_conserved_hypothetical_protein_3272409..3272559_POSITIVE 280 219 173 128 80 tfor:TF2532|3end_ferric-uptake_regulator_2715708..2715858_POSITIVE 279 167 128 61 24 tfor:TF2753|3end_zinc_protease_2940448..2940598_POSITIVE