Origin IGS: gtcaataaaaaattaataatcggaaacaaagatatactcctaaaagctgttatatttttcgaatgaaaccgaatataaataaaacaggtcttatataaatcgtaaaatattgttcgtttgttatttgtcgacttttatagagtaaaaaaatataaatggaataatgatgaaaacagtgcgaccgctttctccgaaggacgtgttttta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taaaaacacgtccttcggagaaagcggtcgcactgttttcatcattattccatttatatttttttactctataaaagtcgacaaataacaaacgaacaatattttacgatttatataagacctgttttatttatattcggtttcattcgaaaaatataacagcttttaggagtatatctttgtttccgattattaattttttattgac Mask Tandem Repeat Region ================================================ gtcaataaaaaattaataatcggaaacaaagatatactcctaaaagctgttatatttttcgaatgaaaccgaatataaataaaacaggtcttatataaatcgtaaaatattgttcgtttgttatttgtcgacttttatagagtaaaaaaatataaatggaataatgatgaaaacagtgcgaccgctttctccgaaggacgtgttttta Find is-nt database================================================ Query_seq: TF0094:TF0095|TF0094:TF0095:alpha-glucosidase:methyltransferase:->->:105825..106032 208 gtcaataaaaaattaataatcggaaacaaagatatactcctaaaagctgttatatttttcgaatgaaaccgaatataaataaaacaggtcttatataaatcgtaaaatattgttcgtttgttatttgtcgacttttatagagtaaaaaaatataaatggaataatgatgaaaacagtgcgaccgctttctccgaaggacgtgttttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF0094:TF0095|TF0094:TF0095:alpha-glucosidase:methyltransferase:->->:105825..106032 208 gtcaataaaaaattaataatcggaaacaaagatatactcctaaaagctgttatatttttcgaatgaaaccgaatataaataaaacaggtcttatataaatcgtaaaatattgttcgtttgttatttgtcgacttttatagagtaaaaaaatataaatggaataatgatgaaaacagtgcgaccgctttctccgaaggacgtgttttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF0094:TF0095|TF0094:TF0095:alpha-glucosidase:methyltransferase:->->:105825..106032 208 gtcaataaaaaattaataatcggaaacaaagatatactcctaaaagctgttatatttttcgaatgaaaccgaatataaataaaacaggtcttatataaatcgtaaaatattgttcgtttgttatttgtcgacttttatagagtaaaaaaatataaatggaataatgatgaaaacagtgcgaccgctttctccgaaggacgtgttttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 gtcaataaaaaattaataatcggaaacaaagatatactcctaaaagctgttatatttttcgaatgaaaccgaatataaataaaacaggtcttatataaatcgtaaaatattgttcgtttgttatttgtcgacttttatagagtaaaaaaatataaatggaataatgatgaaaacagtgcgaccgctttctccgaaggacgtgttttta Predict ORF larger than 30AA ================================================ Protein_Len: 33 Strand: + Start: 109 End: 207 ............................................................................................................ M V R L L F V D F Y R V K K Y K W N N D E N S A T A F S E G R V F . Protein_Len: 35 Strand: - Start: 53 End: 157 .................................................... I K R I F G F I F L V P R I Y I T F Y Q E N T I Q R S K Y L L F I Y M ................................................... Protein_Len: 36 Strand: - Start: 3 End: 110 .. L L F N I I P F L S I S R F A T I N K S H F R I Y I F C T K Y L D Y F M .................................................................................................. gtcaataaaaaattaataatcggaaacaaagatatactcctaaaagctgttatatttttcgaatgaaaccgaatataaataaaacaggtcttatataaatcgtaaaatattgttcgtttgttatttgtcgacttttatagagtaaaaaaatataaatggaataatgatgaaaacagtgcgaccgctttctccgaaggacgtgttttta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ gtcaataaaaaattaataatcggaaacaaagatatactcctaaaagctgttatatttttcgaatgaaaccgaatataaataaaacaggtcttatataaatcgtaaaatattgttcgtttgttatttgtcgacttttatagagtaaaaaaatataaatggaataatgatgaaaacagtgcgaccgctttctccgaaggacgtgttttta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF0094:TF0095|TF0094:TF0095:alpha-glucosidase:methyltransferase:->->:105825..106032 208 gtcaataaaaaattaataatcggaaacaaagatatactcctaaaagctgttatatttttcgaatgaaaccgaatataaataaaacaggtcttatataaatcgtaaaatattgttcgtttgttatttgtcgacttttatagagtaaaaaaatataaatggaataatgatgaaaacagtgcgaccgctttctccgaaggacgtgttttta Intra-Species Hit: Count: 1 Min: 1 Max: 208 Len: 208 Subject: tfor_TF0094_TF0095|alpha- glucosidase:methyltransferase|POSITIVE:POSITIVE|[105825, 106032]|208 HSP 1 e-value: 1.0E-102 bit: 371.0 Len: 208 Query Start:1 Query End:208 Subject Strand: POSITIVE Subject Start: 1 Subject End: 208 gtcaataaaaaattaataatcggaaacaaagatatactcctaaaagctgttatatttttcgaatgaaaccgaatataaataaaacaggtcttatataaatcgtaaaatattgttcgtttgttatttgtcgacttttatagagtnnnnnnntataaatggaataatgatgaaaacagtgcgaccgctttctccgaaggacgtgttttta gtcaataaaaaattaataatcggaaacaaagatatactcctaaaagctgttatatttttcgaatgaaaccgaatataaataaaacaggtcttatataaatcgtaaaatattgttcgtttgttatttgtcgacttttatagagtaaaaaaatataaatggaataatgatgaaaacagtgcgaccgctttctccgaaggacgtgttttta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GUCAAUAAAAAAUUAAUAAUCGGAAACAAAGAUAUACUCCUAAAAGCUGUUAUAUUUUUCGAAUGAAACCGAAUAUAAAUAAAACAGGUCUUAUAUAAAUCGUAAAAUAUUGUUCGUUUGUUAUUUGUCGACUUUUAUAGAGUAAAAAAAUAUAAAUGGAAUAAUGAUGAAAACAGUGCGACCGCUUUCUCCGAAGGACGUGUUUUUA .....................(....)..(((.((((((((.(((((.(((.(((((((((........))))...))))).))).((((.(((.....((((....((((((((((((((.((((....((((.....))))....)))))))))).)))))))))))).....))).)))))))))......)))).)))).))). (-33.20) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAAACACGUCCUUCGGAGAAAGCGGUCGCACUGUUUUCAUCAUUAUUCCAUUUAUAUUUUUUUACUCUAUAAAAGUCGACAAAUAACAAACGAACAAUAUUUUACGAUUUAUAUAAGACCUGUUUUAUUUAUAUUCGGUUUCAUUCGAAAAAUAUAACAGCUUUUAGGAGUAUAUCUUUGUUUCCGAUUAUUAAUUUUUUAUUGAC ((((((........(((((((((((((.....)))))))).....((((((...............((.((((.((((((..(((((............)))))..)))))).))))))..(((((.(((((...(((((......)))))))))).)))))......))))))..........)))))........))))))..... (-37.29) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 333 200 161 218 173 tfor:TF2216|5end_fumarate_hydratase,_class_I_2388227..2388457_POSITIVE 325 199 155 199 140 tfor:TF2224|5end_CTn_excision_protein_2394592..2394822_POSITIVE 300 200 157 62 18 tfor:TF0310|5end_conserved_hypothetical_protein_334685..334915_POSITIVE 297 193 156 196 160 tfor:TF2270|5end_conserved_hypothetical_protein_2435463..2435693_POSITIVE 280 200 151 214 167 tfor:TF0398|5end_Fe-S_oxidoreductase_418984..419214_POSITIVE 278 200 156 90 32 tfor:TF0600|5end_oxidoreductase/dehydrogenase_630083..630313_POSITIVE 271 187 149 189 160 tfor:TF0585|5end_conserved_hypothetical_protein_614782..615012_POSITIVE 266 200 165 159 122 tfor:TF2318|5end_conserved_hypothetical_protein_2475603..2475833_POSITIVE 262 185 155 200 168 tfor:TF1166|5end_ABC_transporter,_permease_component_1246154..1246384_POSITIVE 261 191 152 223 177 tfor:TF2640|5end_hypothetical_protein_2809106..2809336_POSITIVE 261 191 152 137 91 tfor:TF2639|5end_hypothetical_protein_2809020..2809250_POSITIVE 260 200 157 221 192 tfor:TF1056|5end_conserved_hypothetical_protein_1120528..1120758_POSITIVE 258 197 174 28 5 tfor:TF0021|5end_outer_membrane_phospholipase_A_15949..16179_POSITIVE 256 198 156 53 11 tfor:TF2519|5end_membrane_protein,_metallopeptidase_M23/M37_family_2697676..2697906_POSITIVE 254 200 159 201 151 tfor:TF1068|5end_conserved_hypothetical_protein;_possible_N-acetylglucosamine_kinase_1138036..1138266_POSITIVE 254 198 156 215 180 tfor:TF0001|5end_hypothetical_protein_-116..114_POSITIVE 253 197 175 163 132 tfor:TF0555|5end_Bacteroides_aerotolerance_protein,_BatB_587048..587278_POSITIVE 252 199 165 160 114 tfor:TF2264|5end_transfer_region-related_protein,_TraO_2432890..2433120_POSITIVE 251 200 174 38 7 tfor:TF0877|5end_ISPg2-related_(PGIS2-related)_transposase_931477..931707_POSITIVE 250 200 165 71 33 tfor:TF0076|5end_probable_carbohydrate_kinase,_PfkB_family_83279..83509_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 324 193 155 148 100 tfor:TF2223|3end_CTn_excision__protein_2394632..2394782_POSITIVE 300 200 157 72 28 tfor:TF0309|3end_conserved_hypothetical_protein_334775..334925_POSITIVE 297 193 156 126 90 tfor:TF2267|3end_lysozyme-related_protein_2435473..2435623_POSITIVE 271 200 156 57 12 tfor:TF0599|3end_hypothetical_protein_630130..630280_POSITIVE 266 200 165 143 106 tfor:TF2317|3end_3-oxoacyl-[acyl-carrier-protein]_synthase_II_2475667..2475817_POSITIVE 263 200 165 112 68 tfor:TF0624|3end_Xaa-Pro_dipeptidase_(aminopeptidase_P)_661015..661165_POSITIVE 261 191 152 88 42 tfor:TF2638|3end_cation_transport_protein_2809051..2809201_POSITIVE 260 200 157 51 1 tfor:TF2740|3end_conserved_hypothetical_protein_2921972..2922122_POSITIVE 258 197 174 28 5 tfor:TF0020|3end_possible_glycoprotein_endopeptidase_16029..16179_POSITIVE 254 198 156 51 16 tfor:TF3165|3end_thiol:disulfide_interchange_protein,_thioredoxin_family_protein_3405343..3405493_POSITIVE 253 197 175 149 118 tfor:TF0554|3end_Bacteroides_aerotolerance_protein,_BatC_587114..587264_POSITIVE 252 198 156 146 91 tfor:TF1269|3end_acetyltransferase_1355180..1355330_POSITIVE 244 200 164 71 25 tfor:TF1898|3end_RNA_polymerase_ECF-type_sigma_factor_2052770..2052920_POSITIVE 243 198 158 62 19 tfor:TF3067|3end_conserved_hypothetical_protein_3292877..3293027_POSITIVE 243 200 155 47 9 tfor:TF2409|3end_hypothetical_protein_2584155..2584305_POSITIVE 242 200 158 119 66 tfor:TF3157|3end_sodium/solute_symporter_3393787..3393937_POSITIVE 242 194 155 80 27 tfor:TF0884|3end_hypothetical_protein_937850..938000_POSITIVE 242 197 175 150 126 tfor:TF0423|3end_G:T/U_mismatch-specific_DNA_glycosylase_439281..439431_POSITIVE 241 200 154 140 89 tfor:TF3031|3end_conserved_hypothetical_protein_3255086..3255236_POSITIVE 239 200 179 142 121 tfor:TF2703|3end_conserved_hypothetical_protein_2876317..2876467_POSITIVE