Origin IGS: tgaatcttatgatagcaatcgatgaaaaaaatctgataagtattattgctctgatgggtttcggagtgtttaattggatgaggagataaatatcttacacgcctttacatgcaaaaagtcctacaagtatgtgtccgtaggacttttttcttccaccggcttgatcatctcttagcctcccggtagagagcgaggctctttctcttgaaccttcaaacagtataataaacgttcaatactattgtataatagcactttaagtaaagtattaataaaataagatttcatagaaaatctttggtagaatcaattttggggtgtaaat .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 atttacaccccaaaattgattctaccaaagattttctatgaaatcttattttattaatactttacttaaagtgctattatacaatagtattgaacgtttattatactgtttgaaggttcaagagaaagagcctcgctctctaccgggaggctaagagatgatcaagccggtggaagaaaaaagtcctacggacacatacttgtaggactttttgcatgtaaaggcgtgtaagatatttatctcctcatccaattaaacactccgaaacccatcagagcaataatacttatcagatttttttcatcgattgctatcataagattca Mask Tandem Repeat Region ================================================ tgaatcttatgatagcaatcgatgaaaaaaatctgataagtattattgctctgatgggtttcggagtgtttaattggatgaggagataaatatcttacacgcctttacatgcaaaaagtcctacaagtatgtgtccgtaggacttttttcttccaccggcttgatcatctcttagcctcccggtagagagcgaggctctttctcttgaaccttcaaacagtataataaacgttcaatactattgtataatagcactttaagtaaagtattaataaaataagatttcatagaaaatctttggtagaatcaattttggggtgtaaat Find is-nt database================================================ Query_seq: TF0054:TF0055|TF0054:TF0055:possible flavodoxin:integrase fragment, N-terminal:->->:58521..58845 325 tgaatcttatgatagcaatcgatgaaaaaaatctgataagtattattgctctgatgggtttcggagtgtttaattggatgaggagataaatatcttacacgcctttacatgcaaaaagtcctacaagtatgtgtccgtaggacttttttcttccaccggcttgatcatctcttagcctcccggtagagagcgaggctctttctcttgaaccttcaaacagtataataaacgttcaatactattgtataatagcactttaagtaaagtattaataaaataagatttcatagaaaatctttggtagaatcaattttggggtgtaaat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF0054:TF0055|TF0054:TF0055:possible flavodoxin:integrase fragment, N-terminal:->->:58521..58845 325 tgaatcttatgatagcaatcgatgaaaaaaatctgataagtattattgctctgatgggtttcggagtgtttaattggatgaggagataaatatcttacacgcctttacatgcaaaaagtcctacaagtatgtgtccgtaggacttttttcttccaccggcttgatcatctcttagcctcccggtagagagcgaggctctttctcttgaaccttcaaacagtataataaacgttcaatactattgtataatagcactttaagtaaagtattaataaaataagatttcatagaaaatctttggtagaatcaattttggggtgtaaat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF0054:TF0055|TF0054:TF0055:possible flavodoxin:integrase fragment, N-terminal:->->:58521..58845 325 tgaatcttatgatagcaatcgatgaaaaaaatctgataagtattattgctctgatgggtttcggagtgtttaattggatgaggagataaatatcttacacgcctttacatgcaaaaagtcctacaagtatgtgtccgtaggacttttttcttccaccggcttgatcatctcttagcctcccggtagagagcgaggctctttctcttgaaccttcaaacagtataataaacgttcaatactattgtataatagcactttaagtaaagtattaataaaataagatttcatagaaaatctttggtagaatcaattttggggtgtaaat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tgaatcttatgatagcaatcgatgaaaaaaatctgataagtattattgctctgatgggtttcggagtgtttaattggatgaggagataaatatcttacacgcctttacatgcaaaaagtcctacaagtatgtgtccgtaggacttttttcttccaccggcttgatcatctcttagcctcccggtagagagcgaggctctttctcttgaaccttcaaacagtataataaacgttcaatactattgtataatagcactttaagtaaagtattaataaaataagatttcatagaaaatctttggtagaatcaattttggggtgtaaat Predict ORF larger than 30AA ================================================ Protein_Len: 33 Strand: + Start: 74 End: 172 ......................................................................... M D E E I N I L H A F T C K K S Y K Y V S V G L F S S T G L I I S ......................................................................................................................................................... Protein_Len: 38 Strand: - Start: 175 End: 288 .............................................................................................................................................................................. G G P L S R P E K E Q V K L C Y L L R E I S N Y L L V K L L T N I F Y S K M ..................................... Protein_Len: 43 Strand: - Start: 98 End: 226 ................................................................................................. V G K C A F L G V L I H G Y S K K R G G A Q D D R L R G T S L S A R E R S G E F L I M ................................................................................................... Protein_Len: 30 Strand: - Start: 2 End: 91 . F R I I A I S S F F R I L I I A R I P K P T N L Q I L L Y M .......................................................................................................................................................................................................................................... tgaatcttatgatagcaatcgatgaaaaaaatctgataagtattattgctctgatgggtttcggagtgtttaattggatgaggagataaatatcttacacgcctttacatgcaaaaagtcctacaagtatgtgtccgtaggacttttttcttccaccggcttgatcatctcttagcctcccggtagagagcgaggctctttctcttgaaccttcaaacagtataataaacgttcaatactattgtataatagcactttaagtaaagtattaataaaataagatttcatagaaaatctttggtagaatcaattttggggtgtaaat Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tgaatcttatgatagcaatcgatgaaaaaaatctgataagtattattgctctgatgggtttcggagtgtttaattggatgaggagataaatatcttacacgcctttacatgcaaaaagtcctacaagtatgtgtccgtaggacttttttcttccaccggcttgatcatctcttagcctcccggtagagagcgaggctctttctcttgaaccttcaaacagtataataaacgttcaatactattgtataatagcactttaagtaaagtattaataaaataagatttcatagaaaatctttggtagaatcaattttggggtgtaaat Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 87 HP_score: -6.8 Tail_Score: -5.4505 Start: 133 End: 143 Full_Region: GTCCTACAAGTATGT GTCC GTA GGAC TTTTTTCTTCCACCG ....................................................................................................................................GTCCGTAGGAC...................................................................................................................................................................................... TransTerm Strand: + Conf: 71 HP_score: -6.8 Tail_Score: -4.20025 Start: 188 End: 198 Full_Region: TAGCCTCCCGGTAGA GAGC GAG GCTC TTTCTCTTGAACCTT ...........................................................................................................................................................................................GAGCGAGGCTC............................................................................................................................... Find igs database================================================ Query_seq: TF0054:TF0055|TF0054:TF0055:possible flavodoxin:integrase fragment, N-terminal:->->:58521..58845 325 tgaatcttatgatagcaatcgatgaaaaaaatctgataagtattattgctctgatgggtttcggagtgtttaattggatgaggagataaatatcttacacgcctttacatgcaaaaagtcctacaagtatgtgtccgtaggacttttttcttccaccggcttgatcatctcttagcctcccggtagagagcgaggctctttctcttgaaccttcaaacagtataataaacgttcaatactattgtataatagcactttaagtaaagtattaataaaataagatttcatagaaaatctttggtagaatcaattttggggtgtaaat Intra-Species Hit: Count: 1 Min: 1 Max: 325 Len: 325 Subject: tfor_TF0054_TF0055|possible flavodoxin:integrase fragment, N-terminal|POSITIVE:POSITIVE|[58521,58845]|325 HSP 1 e-value: 1.0E-171 bit: 603.0 Len: 325 Query Start:1 Query End:325 Subject Strand: POSITIVE Subject Start: 1 Subject End: 325 tgaatcttatgatagcaatcgatgnnnnnnntctgataagtattattgctctgatgggtttcggagtgtttaattggatgaggagataaatatcttacacgcctttacatgcaaaaagtcctacaagtatgtgtccgtaggacttttttcttccaccggcttgatcatctcttagcctcccggtagagagcgaggctctttctcttgaaccttcaaacagtataataaacgttcaatactattgtataatagcactttaagtaaagtattaataaaataagatttcatagaaaatctttggtagaatcaattttggggtgtaaat tgaatcttatgatagcaatcgatgaaaaaaatctgataagtattattgctctgatgggtttcggagtgtttaattggatgaggagataaatatcttacacgcctttacatgcaaaaagtcctacaagtatgtgtccgtaggacttttttcttccaccggcttgatcatctcttagcctcccggtagagagcgaggctctttctcttgaaccttcaaacagtataataaacgttcaatactattgtataatagcactttaagtaaagtattaataaaataagatttcatagaaaatctttggtagaatcaattttggggtgtaaat Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGAAUCUUAUGAUAGCAAUCGAUGAAAAAAAUCUGAUAAGUAUUAUUGCUCUGAUGGGUUUCGGAGUGUUUAAUUGGAUGAGGAGAUAAAUAUCUUACACGCCUUUACAUGCAAAAAGUCCUACAAGUAUGUGUCCGUAGGACUUUUUUCUUCCACCGGCUUGAUCAUCUCUUAGCCUCCCGGUAGAGAGCGAGGCUCUUUCUCUUGAACCUUCAAACAGUAUAAUAAACGUUCAAUACUAUUGUAUAAUAGCACUUUAAGUAAAGUAUUAAUAAAAUAAGAUUUCAUAGAAAAUCUUUGGUAGAAUCAAUUUUGGGGUGUAAAU .......((..((((..((((((.......))).)))..((.((((((..(((..((((((.((((.........((((((.(((((....))))).((.(((.......(.((((((((((((............)))))))))))).).......))).)).))))))...((((((.(........).))))))....)))).)))))).....)))..))))))))........))))..)).....((((((((((...........((....(((((((......)))))))...))........)))))))))).... (-70.35) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUUUACACCCCAAAAUUGAUUCUACCAAAGAUUUUCUAUGAAAUCUUAUUUUAUUAAUACUUUACUUAAAGUGCUAUUAUACAAUAGUAUUGAACGUUUAUUAUACUGUUUGAAGGUUCAAGAGAAAGAGCCUCGCUCUCUACCGGGAGGCUAAGAGAUGAUCAAGCCGGUGGAAGAAAAAAGUCCUACGGACACAUACUUGUAGGACUUUUUGCAUGUAAAGGCGUGUAAGAUAUUUAUCUCCUCAUCCAAUUAAACACUCCGAAACCCAUCAGAGCAAUAAUACUUAUCAGAUUUUUUUCAUCGAUUGCUAUCAUAAGAUUCA ..((((((.((........(((((((.((((((((....))))))))...(((((....((((.(((..(((((((((....)))))))))((((.((((..........)))).))))..)))))))((((((.(........).))))))....))))).......)))))))..((((((((((((((........)))))))))))))).........)).))))))((((...(((..................(((.((......)).)))...............((......))...)))...)))).......... (-71.20) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 378 214 177 49 6 tfor:TF1908|5end_possible_phosphoglycerate_mutase_2062695..2062925_POSITIVE 375 195 156 53 15 tfor:TF0532|5end_hypothetical_protein_557509..557739_POSITIVE 371 197 151 57 2 tfor:TF2876|5end_cytosine_deaminase_3065144..3065374_POSITIVE 367 199 156 132 83 tfor:TF1003|5end_conserved_hypothetical_protein;_possible_secreted_glycosylhydrolase_1058177..1058407_POSITIVE 363 200 156 92 30 tfor:TF0190|5end_cell_division_protein_FtsH_204029..204259_POSITIVE 359 197 151 168 112 tfor:TF3119|5end_conserved_hypothetical_protein_3352723..3352953_POSITIVE 356 192 153 46 4 tfor:TF0677|5end_ATP-binding_protein,_Mrp/Nbp35_family,_involved_in_chromosome_partitioning_719799..720029_POSITIVE 350 193 156 209 174 tfor:TF1261|5end_GrpE-related_chaperonin;_HSP-70_cofactor_1346504..1346734_POSITIVE 349 168 124 205 156 tfor:TF0381|5end_cell_cycle_protein_401632..401862_POSITIVE 346 200 157 214 162 tfor:TF2346|5end_hypothetical_protein_2509669..2509899_POSITIVE 344 213 174 75 21 tfor:TF2657|5end_conserved_hypothetical_protein_2829729..2829959_POSITIVE 342 196 151 196 147 tfor:TF0992|5end_hypothetical_protein_1048251..1048481_POSITIVE 342 190 144 226 170 tfor:TF0665|5end_conserved_hypothetical_protein_709497..709727_POSITIVE 340 199 155 82 25 tfor:TF2372|5end_acyl-(acyl-carrier-protein)-UDP-N-acetylglucosami_ne_acyltransferase_2537558..2537788_POSITIVE 338 198 154 186 151 tfor:TF1061|5end_glycosyltransferase_1130264..1130494_POSITIVE 335 195 153 126 82 tfor:TF2435|5end_DNA_repair_protein_radC_2614352..2614582_POSITIVE 327 186 143 157 106 tfor:TF2237|5end_two-component_system_response_regulator_involved_in_CTn_2412494..2412724_POSITIVE 326 196 156 225 168 tfor:TF0685|5end_hypothetical_protein_730852..731082_POSITIVE 324 214 175 174 115 tfor:TF0888|5end_transfer_region-related_protein,_TraN_938392..938622_POSITIVE 323 220 177 157 117 tfor:TF1745|5end_hypothetical_protein_1879154..1879384_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 371 197 151 57 2 tfor:TF2874|3end_conserved_hypothetical_protein_3065224..3065374_POSITIVE 367 199 156 107 58 tfor:TF1002|3end_replicative_DNA_helicase_1058232..1058382_POSITIVE 363 200 156 73 11 tfor:TF0189|3end_phosphatidate_cytidylytransferase_(CDP-diglyceride_synthetase)_204090..204240_POSITIVE 358 200 153 110 53 tfor:TF1983|3end_conserved_hypothetical_protein_2135000..2135150_POSITIVE 353 220 171 105 37 tfor:TF3165|3end_thiol:disulfide_interchange_protein,_thioredoxin_family_protein_3405343..3405493_POSITIVE 349 168 124 126 77 tfor:TF0380|3end_conserved_hypothetical_protein_401633..401783_POSITIVE 344 213 174 88 34 tfor:TF2656|3end_valyl-tRNA_synthetase_2829822..2829972_POSITIVE 341 198 156 69 22 tfor:TF0686|3end_hypothetical_protein_732950..733100_POSITIVE 340 199 155 58 1 tfor:TF2371|3end_conserved_hypothetical_protein_2537614..2537764_POSITIVE 335 195 153 57 13 tfor:TF2434|3end_conserved_hypothetical_protein_2614363..2614513_POSITIVE 334 176 133 138 91 tfor:TF0207|3end_conserved_hypothetical_protein_233505..233655_POSITIVE 323 220 177 141 101 tfor:TF1744|3end_phenylacetate-CoA_ligase_1879218..1879368_POSITIVE 320 196 151 62 21 tfor:TF2340|3end_hypothetical_protein_2500915..2501065_POSITIVE 318 195 153 102 60 tfor:TF2640|3end_hypothetical_protein_2809533..2809683_POSITIVE 317 195 153 108 67 tfor:TF2882|3end_conserved_hypothetical_protein_3074401..3074551_POSITIVE 315 205 161 76 33 tfor:TF3102|3end_hypothetical_protein_3332969..3333119_POSITIVE 310 197 152 90 45 tfor:TF2412|3end_outer_membrane_protein_2590393..2590543_POSITIVE 310 195 151 59 6 tfor:TF1197|3end_transcriptional_regulator_1281414..1281564_POSITIVE 308 179 136 143 79 tfor:TF1350|3end_possible_transcriptional_regulator_1430636..1430786_POSITIVE 308 226 182 56 2 tfor:TF0428|3end_methylmalonyl-CoA_mutase,_beta_subunit_450033..450183_POSITIVE