Origin IGS: atatttcttttatcggctcattgtcttctgctccgaaaccaatcatgaaatgcgaaaagacctgctttgaagtcccgaaagccccatttcttaaaagatgtaaaactattca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tgaatagttttacatcttttaagaaatggggctttcgggacttcaaagcaggtcttttcgcatttcatgattggtttcggagcagaagacaatgagccgataaaagaaatat Mask Tandem Repeat Region ================================================ atatttcttttatcggctcattgtcttctgctccgaaaccaatcatgaaatgcgaaaagacctgctttgaagtcccgaaagccccatttcttaaaagatgtaaaactattca Find is-nt database================================================ Query_seq: TF0024:TF0025|TF0024:TF0025:conserved hypothetical protein; possible ATPase:hypothetical protein:->->:21687..21798 112 atatttcttttatcggctcattgtcttctgctccgaaaccaatcatgaaatgcgaaaagacctgctttgaagtcccgaaagccccatttcttaaaagatgtaaaactattca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF0024:TF0025|TF0024:TF0025:conserved hypothetical protein; possible ATPase:hypothetical protein:->->:21687..21798 112 atatttcttttatcggctcattgtcttctgctccgaaaccaatcatgaaatgcgaaaagacctgctttgaagtcccgaaagccccatttcttaaaagatgtaaaactattca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF0024:TF0025|TF0024:TF0025:conserved hypothetical protein; possible ATPase:hypothetical protein:->->:21687..21798 112 atatttcttttatcggctcattgtcttctgctccgaaaccaatcatgaaatgcgaaaagacctgctttgaagtcccgaaagccccatttcttaaaagatgtaaaactattca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 atatttcttttatcggctcattgtcttctgctccgaaaccaatcatgaaatgcgaaaagacctgctttgaagtcccgaaagccccatttcttaaaagatgtaaaactattca Predict ORF larger than 30AA ================================================ Protein_Len: 36 Strand: + Start: 3 End: 110 .. M S F I G S L S S A P K P I M K C E K T C F E V P K A P F L K R C K T I .. Protein_Len: 36 Strand: - Start: 2 End: 109 . I E K I P E N D E A G F G I M F H S F V Q K S T G F A G N R L L H L V M ... atatttcttttatcggctcattgtcttctgctccgaaaccaatcatgaaatgcgaaaagacctgctttgaagtcccgaaagccccatttcttaaaagatgtaaaactattca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ atatttcttttatcggctcattgtcttctgctccgaaaccaatcatgaaatgcgaaaagacctgctttgaagtcccgaaagccccatttcttaaaagatgtaaaactattca Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF0024:TF0025|TF0024:TF0025:conserved hypothetical protein; possible ATPase:hypothetical protein:->->:21687..21798 112 atatttcttttatcggctcattgtcttctgctccgaaaccaatcatgaaatgcgaaaagacctgctttgaagtcccgaaagccccatttcttaaaagatgtaaaactattca Intra-Species Hit: Count: 1 Min: 1 Max: 112 Len: 112 Subject: tfor_TF0024_TF0025|conserved hypothetical protein; possible ATPase:hypothetical protein|POSITIVE:POSITIVE|[21687,21798]|112 HSP 1 e-value: 2.0E-57 bit: 222.0 Len: 112 Query Start:1 Query End:112 Subject Strand: POSITIVE Subject Start: 1 Subject End: 112 atatttcttttatcggctcattgtcttctgctccgaaaccaatcatgaaatgcgaaaagacctgctttgaagtcccgaaagccccatttcttaaaagatgtaaaactattca atatttcttttatcggctcattgtcttctgctccgaaaccaatcatgaaatgcgaaaagacctgctttgaagtcccgaaagccccatttcttaaaagatgtaaaactattca Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUAUUUCUUUUAUCGGCUCAUUGUCUUCUGCUCCGAAACCAAUCAUGAAAUGCGAAAAGACCUGCUUUGAAGUCCCGAAAGCCCCAUUUCUUAAAAGAUGUAAAACUAUUCA .(((.(((((((((((..((........))..))))..........((((((...((((.....)))).......(....)...)))))).))))))).))).......... (-14.20) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGAAUAGUUUUACAUCUUUUAAGAAAUGGGGCUUUCGGGACUUCAAAGCAGGUCUUUUCGCAUUUCAUGAUUGGUUUCGGAGCAGAAGACAAUGAGCCGAUAAAAGAAAUAU ..............(((((((.((((((.((.....(((((((......))))))).)).))))))..........((((..((........))..)))))))))))..... (-23.00) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 348 54 16 85 38 tfor:TF2766|5end_shikimate_kinase_2951619..2951849_POSITIVE 345 78 31 151 105 tfor:TF0406|5end_monofunctional_biosynthetic_peptidoglycan_transglycosylase_424245..424475_POSITIVE 329 97 53 47 2 tfor:TF2547|5end_hypothetical_protein_2730664..2730894_POSITIVE 324 48 4 119 77 tfor:TF0049|5end_membrane-associated_HD_superfamily_hydrolase_55245..55475_POSITIVE 300 45 7 69 30 tfor:TF1816|5end_hypothetical_protein_1957643..1957873_POSITIVE 300 45 7 50 11 tfor:TF1817|5end_hypothetical_protein_1957624..1957854_POSITIVE 297 69 21 173 130 tfor:TF1657|5end_lauroyl/myristoyl_acyltransferase_1772425..1772655_POSITIVE 293 54 14 208 172 tfor:TF2931|5end_3-deoxy-D-manno-2-octulosonic_acid_(Kdo_transferase)_3134275..3134505_POSITIVE 292 97 51 58 14 tfor:TF2326|5end_conserved_hypothetical_protein_2484686..2484916_POSITIVE 288 52 13 82 43 tfor:TF2817|5end_hypothetical_protein_3007358..3007588_POSITIVE 287 54 12 220 177 tfor:TF2466|5end_pyruvate_kinase_2644385..2644615_POSITIVE 287 78 31 164 119 tfor:TF1507|5end_outer_membrane_receptor,_TonB-dependent_1614536..1614766_POSITIVE 284 55 11 71 30 tfor:TF0462|5end_possible_cobalamin_biosynthesis_precorrin-3_methylase_477600..477830_POSITIVE 282 78 31 81 41 tfor:TF2879|5end_tRNA_(guanine-N-1)-methyltransferase_3068254..3068484_POSITIVE 279 57 11 145 100 tfor:TF0505|5end_4-diphosphocytidyl-2-C-methyl-D-erythritol_kinase_530223..530453_POSITIVE 277 50 6 67 21 tfor:TF1221|5end_conserved_hypothetical_protein_1307723..1307953_POSITIVE 277 54 13 56 17 tfor:TF0538|5end_hypothetical_protein_566891..567121_POSITIVE 277 54 13 49 10 tfor:TF0539|5end_hypothetical_protein_566884..567114_POSITIVE 275 59 15 103 49 tfor:TF2878|5end_conserved_hypothetical_protein;_possible_CAAX_amino_terminal_protease_family_3067304..3067534_POSITIVE 275 88 45 204 160 tfor:TF2294|5end_hypothetical_protein_2448012..2448242_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 345 78 31 134 88 tfor:TF0405|3end_conserved_hypothetical_protein_424308..424458_POSITIVE 324 48 4 94 52 tfor:TF0050|3end_hypothetical_protein_55300..55450_POSITIVE 296 77 34 81 34 tfor:TF2953|3end_possible_TonB-dependent_outer_membrane_receptor_3161501..3161651_POSITIVE 288 85 53 38 1 tfor:TF2900|3end_conserved_hypothetical_protein_3090029..3090179_POSITIVE 279 57 11 145 100 tfor:TF0504|3end_Fe-S_oxidoreductase,_family_2_530303..530453_POSITIVE 277 50 6 61 15 tfor:TF1220|3end_conserved_hypothetical_protein_1307797..1307947_POSITIVE 275 77 33 46 2 tfor:TF1726|3end_hypothetical_protein_1852281..1852431_POSITIVE 273 77 31 67 14 tfor:TF2171|3end_hypothetical_protein_2345792..2345942_POSITIVE 271 84 42 84 29 tfor:TF0877|3end_ISPg2-related_(PGIS2-related)_transposase_931817..931967_POSITIVE 267 54 13 148 108 tfor:TF1186|3end_hypothetical_protein_1269815..1269965_POSITIVE 261 84 51 72 46 tfor:TF2974|3end_conserved_hypothetical_protein;_possible_hydrolase_3182439..3182589_POSITIVE 255 68 25 122 54 tfor:TF0901|3end_conserved_hypothetical_protein_947273..947423_POSITIVE 254 84 49 70 37 tfor:TF0542|3end_hypothetical_protein_572324..572474_POSITIVE 253 107 64 54 14 tfor:TF1160|3end_MutT/nudix_family_protein_1241008..1241158_POSITIVE 253 77 32 144 99 tfor:TF0174|3end_hypothetical_protein_185600..185750_POSITIVE 250 72 35 113 72 tfor:TF2018|3end_hypothetical_protein_2175315..2175465_POSITIVE 248 54 16 96 62 tfor:TF2549|3end_30S_ribosomal_protein_S7_2732345..2732495_POSITIVE 248 35 1 46 6 tfor:TF0392|3end_hypothetical_protein_412945..413095_POSITIVE 247 60 13 81 21 tfor:TF3071|3end_integral_membrane_protein,_possible_MotA/TolQ/ExbB_proton_channel_family_3295382..3295532_POSITIVE 246 101 61 61 24 tfor:TF0762|3end_chromate_transport_protein_809755..809905_POSITIVE