Origin IGS: taaccattaaccattgaccattaacaactgaattacgtcaggctttcagccctccgctgagggggggcgcccttttttcccaacgcttcgcttcgctccacattgggctgaattaatgcgccctttcagggctgttacaacggtcagcaaacacctcaacaattcaacacctcaacacctcaacacctcaacgtctcaacacctcaatgccccgaaagggttagcccgcccgaggcccaagggacgaacgccgaggcgttcggtcttttctgttttctgggcgtttattcctgcaataccgcagaagggaaacagaaaaagagaatatatcttttttatttgtttttacaaaatataaatcatgtgaagttatatttatggtataaatttaagaactctgccccgggaaccccggtttccgcggcagagatgtgttta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taaacacatctctgccgcggaaaccggggttcccggggcagagttcttaaatttataccataaatataacttcacatgatttatattttgtaaaaacaaataaaaaagatatattctctttttctgtttcccttctgcggtattgcaggaataaacgcccagaaaacagaaaagaccgaacgcctcggcgttcgtcccttgggcctcgggcgggctaaccctttcggggcattgaggtgttgagacgttgaggtgttgaggtgttgaggtgttgaattgttgaggtgtttgctgaccgttgtaacagccctgaaagggcgcattaattcagcccaatgtggagcgaagcgaagcgttgggaaaaaagggcgccccccctcagcggagggctgaaagcctgacgtaattcagttgttaatggtcaatggttaatggtta Mask Tandem Repeat Region ================================================ taaccattaaccattgaccattaacaactgaattacgtcaggctttcagccctccgctgagggggggcgcccttttttcccaacgcttcgcttcgctccacattgggctgaattaatgcgccctttcagggctgttacaacggtcagcannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntgccccgaaagggttagcccgcccgaggcccaagggacgaacgccgaggcgttcggtcttttctgttttctgggcgtttattcctgcaataccgcagaagggaaacagaaaaagagaatatatcttttttatttgtttttacaaaatataaatcatgtgaagttatatttatggtataaatttaagaactctgccccgggaaccccggtttccgcggcagagatgtgttta Find is-nt database================================================ Query_seq: TF0018:TF0019|TF0018:TF0019:hypothetical protein:heat shock protein ClpB:->->:12152..12589 438 taaccattaaccattgaccattaacaactgaattacgtcaggctttcagccctccgctgagggggggcgcccttttttcccaacgcttcgcttcgctccacattgggctgaattaatgcgccctttcagggctgttacaacggtcagcaaacacctcaacaattcaacacctcaacacctcaacacctcaacgtctcaacacctcaatgccccgaaagggttagcccgcccgaggcccaagggacgaacgccgaggcgttcggtcttttctgttttctgggcgtttattcctgcaataccgcagaagggaaacagaaaaagagaatatatcttttttatttgtttttacaaaatataaatcatgtgaagttatatttatggtataaatttaagaactctgccccgggaaccccggtttccgcggcagagatgtgttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF0018:TF0019|TF0018:TF0019:hypothetical protein:heat shock protein ClpB:->->:12152..12589 438 taaccattaaccattgaccattaacaactgaattacgtcaggctttcagccctccgctgagggggggcgcccttttttcccaacgcttcgcttcgctccacattgggctgaattaatgcgccctttcagggctgttacaacggtcagcaaacacctcaacaattcaacacctcaacacctcaacacctcaacgtctcaacacctcaatgccccgaaagggttagcccgcccgaggcccaagggacgaacgccgaggcgttcggtcttttctgttttctgggcgtttattcctgcaataccgcagaagggaaacagaaaaagagaatatatcttttttatttgtttttacaaaatataaatcatgtgaagttatatttatggtataaatttaagaactctgccccgggaaccccggtttccgcggcagagatgtgttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF0018:TF0019|TF0018:TF0019:hypothetical protein:heat shock protein ClpB:->->:12152..12589 438 taaccattaaccattgaccattaacaactgaattacgtcaggctttcagccctccgctgagggggggcgcccttttttcccaacgcttcgcttcgctccacattgggctgaattaatgcgccctttcagggctgttacaacggtcagcaaacacctcaacaattcaacacctcaacacctcaacacctcaacgtctcaacacctcaatgccccgaaagggttagcccgcccgaggcccaagggacgaacgccgaggcgttcggtcttttctgttttctgggcgtttattcctgcaataccgcagaagggaaacagaaaaagagaatatatcttttttatttgtttttacaaaatataaatcatgtgaagttatatttatggtataaatttaagaactctgccccgggaaccccggtttccgcggcagagatgtgttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaccattaaccattgaccattaacaactgaattacgtcaggctttcagccctccgctgagggggggcgcccttttttcccaacgcttcgcttcgctccacattgggctgaattaatgcgccctttcagggctgttacaacggtcagcaaacacctcaacaattcaacacctcaacacctcaacacctcaacgtctcaacacctcaatgccccgaaagggttagcccgcccgaggcccaagggacgaacgccgaggcgttcggtcttttctgttttctgggcgtttattcctgcaataccgcagaagggaaacagaaaaagagaatatatcttttttatttgtttttacaaaatataaatcatgtgaagttatatttatggtataaatttaagaactctgccccgggaaccccggtttccgcggcagagatgtgttta Predict ORF larger than 30AA ================================================ Protein_Len: 30 Strand: + Start: 132 End: 221 ................................................................................................................................... M L Q R S A N T S T I Q H L N T S T P Q R L N T S M P R K G ......................................................................................................................................................................................................................... Protein_Len: 60 Strand: + Start: 32 End: 355 ............................... M T S G F Q P S A E G G R P F F P T L R F A P H W A E L M R P F R A V T T V S K H L N N S T P Q H L N T S T S Q H L N A ................................................................................... Protein_Len: 38 Strand: + Start: 270 End: 383 ............................................................................................................................................................................................................................................................................. M F S G R L F L Q Y R R R E T E K E N I S F L F V F T K Y K S C E V I F M V ....................................................... Protein_Len: 60 Strand: + Start: 112 End: 438 ............................................................................................................... M N A P F Q G C Y N G Q Q T P Q Q F N T S T P Q H L N V S T P Q C P E R V S P P E A Q G T N A E A F G L F C F L G V Y S Protein_Len: 55 Strand: - Start: 224 End: 388 ............................................................................................................................................................................................................................... G A R P G L S S R R P T R D K R N E P R K N R C Y R L L S V S F S F I D K K N T K V F Y L D H S T I N I T Y M .................................................. Protein_Len: 47 Strand: - Start: 199 End: 339 ...................................................................................................................................................................................................... C R L A G F P N A R G L G L P R V G L R E T K E T K Q A N I G A I G C F P F L F L S Y I K K M ................................................................................................... Protein_Len: 38 Strand: - Start: 49 End: 162 ................................................ G E A S P P R G K K G V S R K A G C Q A S N I R G K L A T V V T L L C R L M .................................................................................................................................................................................................................................................................................... taaccattaaccattgaccattaacaactgaattacgtcaggctttcagccctccgctgagggggggcgcccttttttcccaacgcttcgcttcgctccacattgggctgaattaatgcgccctttcagggctgttacaacggtcagcaaacacctcaacaattcaacacctcaacacctcaacacctcaacgtctcaacacctcaatgccccgaaagggttagcccgcccgaggcccaagggacgaacgccgaggcgttcggtcttttctgttttctgggcgtttattcctgcaataccgcagaagggaaacagaaaaagagaatatatcttttttatttgtttttacaaaatataaatcatgtgaagttatatttatggtataaatttaagaactctgccccgggaaccccggtttccgcggcagagatgtgttta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaccattaaccattgaccattaacaactgaattacgtcaggctttcagccctccgctgagggggggcgcccttttttcccaacgcttcgcttcgctccacattgggctgaattaatgcgccctttcagggctgttacaacggtcagcaaacacctcaacaattcaacacctcaacacctcaacacctcaacgtctcaacacctcaatgccccgaaagggttagcccgcccgaggcccaagggacgaacgccgaggcgttcggtcttttctgttttctgggcgtttattcctgcaataccgcagaagggaaacagaaaaagagaatatatcttttttatttgtttttacaaaatataaatcatgtgaagttatatttatggtataaatttaagaactctgccccgggaaccccggtttccgcggcagagatgtgttta Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 100 HP_score: -8.8 Tail_Score: -4.94487 Start: 242 End: 265 Full_Region: CGCCCGAGGCCCAAG GGACGAACGC CGAG GCGTTCGGTC TTTTCTGTTTTCTGG .................................................................................................................................................................................................................................................GGACGAACGCCGAGGCGTTCGGTC............................................................................................................................................................................. TransTerm Strand: + Conf: 75 HP_score: -11.3 Tail_Score: -3.01831 Start: 245 End: 262 Full_Region: CCGAGGCCCAAGGGA CGAACGC CGAG GCGTTCG GTCTTTTCTGTTTTC ....................................................................................................................................................................................................................................................CGAACGCCGAGGCGTTCG................................................................................................................................................................................ TransTerm Strand: - Conf: 73 HP_score: -11.3 Tail_Score: -2.53263 Start: 245 End: 262 Full_Region: gaaaacagaaaagac cgaacgc ctcg gcgttcg tcccttgggcctcgg ....................................................................................................................................................................................................................................................cgaacgcctcggcgttcg................................................................................................................................................................................ TransTerm Strand: + Conf: 79 HP_score: -10.5 Tail_Score: -3.53012 Start: 403 End: 415 Full_Region: TTTAAGAACTCTGCC CCGGG AAC CCCGG TTTCCGCGGCAGAGA ..................................................................................................................................................................................................................................................................................................................................................................................................................CCGGGAACCCCGG....................... Find igs database================================================ Query_seq: TF0018:TF0019|TF0018:TF0019:hypothetical protein:heat shock protein ClpB:->->:12152..12589 438 taaccattaaccattgaccattaacaactgaattacgtcaggctttcagccctccgctgagggggggcgcccttttttcccaacgcttcgcttcgctccacattgggctgaattaatgcgccctttcagggctgttacaacggtcagcaaacacctcaacaattcaacacctcaacacctcaacacctcaacgtctcaacacctcaatgccccgaaagggttagcccgcccgaggcccaagggacgaacgccgaggcgttcggtcttttctgttttctgggcgtttattcctgcaataccgcagaagggaaacagaaaaagagaatatatcttttttatttgtttttacaaaatataaatcatgtgaagttatatttatggtataaatttaagaactctgccccgggaaccccggtttccgcggcagagatgtgttta Intra-Species Hit: Count: 10 Min: 1 Max: 438 Len: 438 Subject: tfor_TF0018_TF0019|hypothetical protein:heat shock protein ClpB|POSITIVE:POSITIVE|[12152,12589]|438 HSP 1 e-value: 6.0E-64 bit: 246.0 Len: 124 Query Start:208 Query End:331 Subject Strand: POSITIVE Subject Start: 208 Subject End: 331 ...............................................................................................................................................................................................................tgccccgaaagggttagcccgcccgaggcccaagggacgaacgccgaggcgttcggtcttttctgttttctgggcgtttattcctgcaataccgcagaagggaaacagaaaaagagaatatatc........................................................................................................... ...............................................................................................................................................................................................................tgccccgaaagggttagcccgcccgaggcccaagggacgaacgccgaggcgttcggtcttttctgttttctgggcgtttattcctgcaataccgcagaagggaaacagaaaaagagaatatatc........................................................................................................... HSP 2 e-value: 1.0E-58 bit: 228.0 Len: 136 Query Start:1 Query End:136 Subject Strand: POSITIVE Subject Start: 1 Subject End: 136 taaccattaaccattgaccattaacaactgaattacgtcaggctttcagccctccgctgannnnnnncgcccttttttcccaacgcttcgcttcgctccacattgggctgaattaatgcgccctttcagggctgtt.............................................................................................................................................................................................................................................................................................................. taaccattaaccattgaccattaacaactgaattacgtcaggctttcagccctccgctgagggggggcgcccttttttcccaacgcttcgcttcgctccacattgggctgaattaatgcgccctttcagggctgtt.............................................................................................................................................................................................................................................................................................................. HSP 3 e-value: 3.0E-44 bit: 180.0 Len: 91 Query Start:348 Query End:438 Subject Strand: POSITIVE Subject Start: 348 Subject End: 438 ...........................................................................................................................................................................................................................................................................................................................................................acaaaatataaatcatgtgaagttatatttatggtataaatttaagaactctgccccgggaaccccggtttccgcggcagagatgtgttta ...........................................................................................................................................................................................................................................................................................................................................................acaaaatataaatcatgtgaagttatatttatggtataaatttaagaactctgccccgggaaccccggtttccgcggcagagatgtgttta Subject: tfor_TF0685_TF0686|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[731193,732338]|1146 HSP 1 e-value: 2.0E-32 bit: 141.0 Len: 120 Query Start:17 Query End:136 Subject Strand: POSITIVE Subject Start: 749 Subject End: 867 ................accattaacaactgaattacgtcaggctttcagccctccgctgannnnnnncgcccttttttcccaacgcttcgcttcgctccacattgggctgaattaatgcgccctttcagggctgtt.............................................................................................................................................................................................................................................................................................................. ................accattaacaattgaattacgtcaggcttacagccctccgccgagatgcgatgcc-ttttttcccaacgcttcgctgcgctccacattgggctgaattaatgcgccctttcagggctgtt.............................................................................................................................................................................................................................................................................................................. Subject: tfor_TF2500_TF2501|ABC transporter, ATP-binding protein:DNA mismatch repair protein|POSITIVE:POSITIVE|[2675363,2676419]|1057 HSP 1 e-value: 4.0E-28 bit: 127.0 Len: 126 Query Start:8 Query End:133 Subject Strand: NEGATIVE Subject Start: 212 Subject End: 334 .......taaccattgaccattaacaactgaattacgtcaggctttcagccctccgctgannnnnnncgcccttttttcccaacgcttcgcttcgctccacattgggctgaattaatgcgccctttcagggct................................................................................................................................................................................................................................................................................................................. .......taacaattgacaattaacaactgaattacgtcaggctttcatccctccgccgaggggg--cgcc-tgttttcccaacgcttcgcttcgctctgcattgggctgaattaatgcgccctttcagggct................................................................................................................................................................................................................................................................................................................. Subject: tfor_TF2438_TF2439|hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[2616218,2616519]|302 HSP 1 e-value: 9.0E-26 bit: 119.0 Len: 114 Query Start:20 Query End:133 Subject Strand: POSITIVE Subject Start: 100 Subject End: 211 ...................attaacaactgaattacgtcaggctttcagccctccgctgannnnnnncgcccttttttcccaacgcttcgcttcgctccacattgggctgaattaatgcgccctttcagggct................................................................................................................................................................................................................................................................................................................. ...................attaacaattaaattatgtcaggctttcagccctccgctgagggggt-cgcc-tgttttcccaacgcttcgcttcgctacacattgggctgaattaatgcgccctttcagggct................................................................................................................................................................................................................................................................................................................. Subject: tfor_TF2815_TF2816|meso-diaminopimelate D-dehydrogenase:hypothetical protein|POSITIVE:POSITIVE|[3006481,3007064]|584 HSP 1 e-value: 3.0E-25 bit: 117.0 Len: 59 Query Start:75 Query End:133 Subject Strand: POSITIVE Subject Start: 232 Subject End: 290 ..........................................................................ttttcccaacgcttcgcttcgctccacattgggctgaattaatgcgccctttcagggct................................................................................................................................................................................................................................................................................................................. ..........................................................................ttttcccaacgcttcgcttcgctccacattgggctgaattaatgcgccctttcagggct................................................................................................................................................................................................................................................................................................................. HSP 2 e-value: 1.0E-12 bit: 75.8 Len: 54 Query Start:1 Query End:54 Subject Strand: NEGATIVE Subject Start: 464 Subject End: 517 taaccattaaccattgaccattaacaactgaattacgtcaggctttcagccctc................................................................................................................................................................................................................................................................................................................................................................................................ taacaattaacaattgaccattaacaattgaattatgtcaggctttcagccctc................................................................................................................................................................................................................................................................................................................................................................................................ Subject: tfor_TF2998_TF2999|surface antigen BspA:conserved hypothetical protein|POSITIVE:POSITIVE|[3215212,3215705]|494 HSP 1 e-value: 8.0E-23 bit: 109.0 Len: 117 Query Start:17 Query End:133 Subject Strand: POSITIVE Subject Start: 188 Subject End: 303 ................accattaacaactgaattacgtcaggctttcagccctccgctgannnnnnncgcccttttttcccaacgcttcgcttcgctccacattgggctgaattaatgcgccctttcagggct................................................................................................................................................................................................................................................................................................................. ................accattaaccattgaattatgtcaggctttcagccctccgccgagggggt-cgccctgttttcccaacgcttcgcttcgctacgcattgggctgaattattacgccctttcagggct................................................................................................................................................................................................................................................................................................................. Subject: tfor_TF1762_TF1763|hypothetical protein:K+-dependent Na+/Ca+ exchanger related-protein|POSITIVE:POSITIVE|[1899709,1900545]|837 HSP 1 e-value: 8.0E-23 bit: 109.0 Len: 63 Query Start:71 Query End:133 Subject Strand: POSITIVE Subject Start: 46 Subject End: 108 ......................................................................ccttttttcccaacgcttcgcttcgctccacattgggctgaattaatgcgccctttcagggct................................................................................................................................................................................................................................................................................................................. ......................................................................ccttttttcccaacgcttcgcttcgctgcacattgggctgaattaacgcgccctttcagggct................................................................................................................................................................................................................................................................................................................. HSP 2 e-value: 3.0E-7 bit: 58.0 Len: 29 Query Start:29 Query End:57 Subject Strand: POSITIVE Subject Start: 2 Subject End: 30 ............................tgaattacgtcaggctttcagccctccgc............................................................................................................................................................................................................................................................................................................................................................................................. ............................tgaattacgtcaggctttcagccctccgc............................................................................................................................................................................................................................................................................................................................................................................................. HSP 3 e-value: 4.0E-6 bit: 54.0 Len: 35 Query Start:209 Query End:243 Subject Strand: POSITIVE Subject Start: 201 Subject End: 235 ................................................................................................................................................................................................................gccccgaaagggttagcccgcccgaggcccaaggg................................................................................................................................................................................................... ................................................................................................................................................................................................................gccccgaaggggttagctcgcccgaggcccaaggg................................................................................................................................................................................................... Subject: tfor_TF2914_TF2915|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[3109704,3110561]|858 HSP 1 e-value: 1.0E-21 bit: 105.0 Len: 57 Query Start:1 Query End:57 Subject Strand: POSITIVE Subject Start: 321 Subject End: 377 taaccattaaccattgaccattaacaactgaattacgtcaggctttcagccctccgc............................................................................................................................................................................................................................................................................................................................................................................................. taaccattaaccattgacaattaacaactgaattacgtcaggctttcagccctccgc............................................................................................................................................................................................................................................................................................................................................................................................. HSP 2 e-value: 1.0E-9 bit: 65.9 Len: 45 Query Start:89 Query End:133 Subject Strand: POSITIVE Subject Start: 402 Subject End: 446 ........................................................................................cgcttcgctccacattgggctgaattaatgcgccctttcagggct................................................................................................................................................................................................................................................................................................................. ........................................................................................cgcttcgctgcacattgggctgaattaacgtgccctttcagggct................................................................................................................................................................................................................................................................................................................. HSP 3 e-value: 4.0E-9 bit: 63.9 Len: 48 Query Start:209 Query End:256 Subject Strand: POSITIVE Subject Start: 490 Subject End: 537 ................................................................................................................................................................................................................gccccgaaagggttagcccgcccgaggcccaagggacgaacgccgagg...................................................................................................................................................................................... ................................................................................................................................................................................................................gccccgaaggggttagctcgaccgaggcccatgggacgaacgccgagg...................................................................................................................................................................................... Subject: tfor_TF2920_TF2921|conserved hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[3117231,3118637]|1407 HSP 1 e-value: 3.0E-19 bit: 97.6 Len: 61 Query Start:76 Query End:136 Subject Strand: POSITIVE Subject Start: 547 Subject End: 607 ...........................................................................tttcccaacgcttcgcttcgctccacattgggctgaattaatgcgccctttcagggctgtt.............................................................................................................................................................................................................................................................................................................. ...........................................................................tttcccaacgcttcgcttcgctgcacattggactgaattaacgcgccctttcagggctgtt.............................................................................................................................................................................................................................................................................................................. HSP 2 e-value: 8.0E-17 bit: 89.7 Len: 61 Query Start:73 Query End:133 Subject Strand: POSITIVE Subject Start: 867 Subject End: 927 ........................................................................ttttttcccaacgcttcgcttcgctccacattgggctgaattaatgcgccctttcagggct................................................................................................................................................................................................................................................................................................................. ........................................................................tttttttccaacgcttcgcttcgctgcgcattgggctgaattaatacgccctttcagggct................................................................................................................................................................................................................................................................................................................. HSP 3 e-value: 4.0E-6 bit: 54.0 Len: 35 Query Start:209 Query End:243 Subject Strand: POSITIVE Subject Start: 995 Subject End: 1029 ................................................................................................................................................................................................................gccccgaaagggttagcccgcccgaggcccaaggg................................................................................................................................................................................................... ................................................................................................................................................................................................................gccccgaaggggttagctcgcccgaggcccaaggg................................................................................................................................................................................................... Subject: tfor_TF1848_TF1850|conserved hypothetical protein:Na+/H+-exchanging protein|POSITIVE:POSITIVE|[1998005,1998681]|677 HSP 1 e-value: 1.0E-18 bit: 95.6 Len: 60 Query Start:1 Query End:60 Subject Strand: NEGATIVE Subject Start: 148 Subject End: 207 taaccattaaccattgaccattaacaactgaattacgtcaggctttcagccctccgctga.......................................................................................................................................................................................................................................................................................................................................................................................... taacaattaaccattgacaattaacaattgaattacgtcaggctttcagccctccgctga.......................................................................................................................................................................................................................................................................................................................................................................................... HSP 2 e-value: 1.0E-15 bit: 85.7 Len: 55 Query Start:79 Query End:133 Subject Strand: NEGATIVE Subject Start: 78 Subject End: 132 ..............................................................................cccaacgcttcgcttcgctccacattgggctgaattaatgcgccctttcagggct................................................................................................................................................................................................................................................................................................................. ..............................................................................cccaacacttcgctgcgctccacattgggctgaattaacgcgccctttcagggct................................................................................................................................................................................................................................................................................................................. Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAACCAUUAACCAUUGACCAUUAACAACUGAAUUACGUCAGGCUUUCAGCCCUCCGCUGAGGGGGGGCGCCCUUUUUUCCCAACGCUUCGCUUCGCUCCACAUUGGGCUGAAUUAAUGCGCCCUUUCAGGGCUGUUACAACGGUCAGCAAACACCUCAACAAUUCAACACCUCAACACCUCAACACCUCAACGUCUCAACACCUCAAUGCCCCGAAAGGGUUAGCCCGCCCGAGGCCCAAGGGACGAACGCCGAGGCGUUCGGUCUUUUCUGUUUUCUGGGCGUUUAUUCCUGCAAUACCGCAGAAGGGAAACAGAAAAAGAGAAUAUAUCUUUUUUAUUUGUUUUUACAAAAUAUAAAUCAUGUGAAGUUAUAUUUAUGGUAUAAAUUUAAGAACUCUGCCCCGGGAACCCCGGUUUCCGCGGCAGAGAUGUGUUUA ...............((((........((((......)))).......(((((((......))))))).............((((((((((((((.(((...(((((((........((((((((((.((((.........(((........))).....................................((....))........)))).))))))))..))........))))))).))))))).).))))))))).)))).((((((((((((..(((..((((.....)))).)))....))))))))))))....(((((((((.((((((...((((.(((.((((((((.((....))..))))))))...))).)))).)))))).((((((.(((((((....))))))).))))))))))))))). (-135.29) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAACACAUCUCUGCCGCGGAAACCGGGGUUCCCGGGGCAGAGUUCUUAAAUUUAUACCAUAAAUAUAACUUCACAUGAUUUAUAUUUUGUAAAAACAAAUAAAAAAGAUAUAUUCUCUUUUUCUGUUUCCCUUCUGCGGUAUUGCAGGAAUAAACGCCCAGAAAACAGAAAAGACCGAACGCCUCGGCGUUCGUCCCUUGGGCCUCGGGCGGGCUAACCCUUUCGGGGCAUUGAGGUGUUGAGACGUUGAGGUGUUGAGGUGUUGAGGUGUUGAAUUGUUGAGGUGUUUGCUGACCGUUGUAACAGCCCUGAAAGGGCGCAUUAAUUCAGCCCAAUGUGGAGCGAAGCGAAGCGUUGGGAAAAAAGGGCGCCCCCCCUCAGCGGAGGGCUGAAAGCCUGACGUAAUUCAGUUGUUAAUGGUCAAUGGUUAAUGGUUA .........(((((((.(((.(((....))).))).))))))).............(((((.(((...((((((.(..(((((...(((((....)))))..............((((.((((((((((....((((.((..((.........))..)))))))))))))))).(((.((((((((((((((((.((((..(((((((((.(((....))).)))))))).)..))).).))).)))))))))))))..)))...))))...)))))..)))))))......(((((((..(((((((..((((.((((((..........((((((((...((...))...))))))))........)))))).......(((..((((.....))))..)))..))))))))))))))))))...))).))))).. (-161.77) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 919 140 91 183 134 tfor:TF2517|5end_hypothetical_protein_2695089..2695319_POSITIVE 911 130 81 120 71 tfor:TF0538|5end_hypothetical_protein_566891..567121_POSITIVE 911 130 81 113 64 tfor:TF0539|5end_hypothetical_protein_566884..567114_POSITIVE 833 90 41 181 135 tfor:TF2478|5end_hypothetical_protein_2655375..2655605_POSITIVE 824 149 101 206 157 tfor:TF2824|5end_nucleoside-transporting_protein_nupG_3014701..3014931_POSITIVE 815 262 226 42 5 tfor:TF3105|5end_hypothetical_protein_3338068..3338298_POSITIVE 803 148 101 231 184 tfor:TF1184|5end_hypothetical_protein_1269004..1269234_POSITIVE 780 262 224 42 1 tfor:TF1187|5end_hypothetical_protein_1269625..1269855_POSITIVE 780 262 224 152 111 tfor:TF1182|5end_hypothetical_protein_1268565..1268795_POSITIVE 767 147 101 203 157 tfor:TF1183|5end_hypothetical_protein_1268391..1268621_POSITIVE 747 119 73 97 51 tfor:TF1716|5end_hypothetical_protein_1837442..1837672_POSITIVE 747 130 81 113 63 tfor:TF0679|5end_hypothetical_protein_721155..721385_POSITIVE 747 130 81 61 11 tfor:TF0678|5end_hypothetical_protein_721103..721333_POSITIVE 709 149 101 209 161 tfor:TF1186|5end_hypothetical_protein_1269568..1269798_POSITIVE 699 120 71 189 146 tfor:TF1406|5end_transposase_1481758..1481988_POSITIVE 680 90 41 64 16 tfor:TF3107|5end_hypothetical_protein_3338459..3338689_POSITIVE 663 130 84 120 74 tfor:TF0534|5end_hypothetical_protein_559509..559739_POSITIVE 652 254 212 78 40 tfor:TF2817|5end_hypothetical_protein_3007358..3007588_POSITIVE 649 139 94 205 160 tfor:TF0828|5end_hypothetical_protein_887090..887320_POSITIVE 646 90 47 45 4 tfor:TF0821|5end_hypothetical_protein_879879..880109_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 820 149 101 86 38 tfor:TF1182|3end_hypothetical_protein_1268938..1269088_POSITIVE 762 260 212 150 105 tfor:TF1186|3end_hypothetical_protein_1269815..1269965_POSITIVE 743 136 91 85 40 tfor:TF2840|3end_hypothetical_protein_3031289..3031439_POSITIVE 715 90 41 113 65 tfor:TF2547|3end_hypothetical_protein_2730947..2731097_POSITIVE 709 149 101 124 76 tfor:TF1185|3end_hypothetical_protein_1269563..1269713_POSITIVE 680 90 41 133 85 tfor:TF3106|3end_hypothetical_protein_3338608..3338758_POSITIVE 663 130 84 50 4 tfor:TF0533|3end_hypothetical_protein_559519..559669_POSITIVE 637 136 94 64 22 tfor:TF0820|3end_hypothetical_protein_879942..880092_POSITIVE 601 59 13 104 55 tfor:TF2816|3end_hypothetical_protein_3007184..3007334_POSITIVE 493 180 131 112 58 tfor:TF0448|3end_hypothetical_protein_467911..468061_POSITIVE 479 90 41 140 91 tfor:TF1405|3end_conserved_hypothetical_protein_1481819..1481969_POSITIVE 469 268 227 78 26 tfor:TF1167|3end_hypothetical_protein_1248932..1249082_POSITIVE 469 98 62 41 4 tfor:TF0370|3end_hypothetical_protein_386317..386467_POSITIVE 467 74 38 137 88 tfor:TF1274|3end_hypothetical_protein_1357895..1358045_POSITIVE 456 64 24 147 110 tfor:TF0678|3end_hypothetical_protein_721335..721485_POSITIVE 450 265 228 145 115 tfor:TF3087|3end_conserved_hypothetical_protein_3322582..3322732_POSITIVE 430 60 13 54 15 tfor:TF0340|3end_hypothetical_protein_364257..364407_POSITIVE 429 220 176 43 1 tfor:TF1657|3end_lauroyl/myristoyl_acyltransferase_1773407..1773557_POSITIVE 429 99 53 61 13 tfor:TF1255|3end_conserved_hypothetical_protein_1338151..1338301_POSITIVE 425 187 141 64 14 tfor:TF2839|3end_ATP-dependent_exodeoxyribonuclease_3031035..3031185_POSITIVE