Origin IGS: tagaagaaaacttaaataaaaaaacaagtcgacttaagagaatctcccttctgctatttttatcattttacttgtaactcccattcttttggagtagaatgaagttagataaaagagggaggtcatcttatgaaaaatctctttagaatccattttacagcaattgcagttatcgatttgctactattcgcattttttaacaaaagacccgagacatctttggagtggctcttgctatctggttttattttcctccttactcaaggactcctgttatttcgcttggttgtccgactcaaacatcaattcgctgagatttatcctcaaatcaataaaaagattcgcttctactatttaggggtgctcatcagcgactttctattttttgtccttttagccttcgttagttctcatcgtttttcatctcttatgccaatcatcactgcttgccattctactttttattatatgacagctgaccacctaagagaaaactatccagatttttacgacaaacacatctctttatgggagtgtctataaggaaaaggaggttttagc .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 gctaaaacctccttttccttatagacactcccataaagagatgtgtttgtcgtaaaaatctggatagttttctcttaggtggtcagctgtcatataataaaaagtagaatggcaagcagtgatgattggcataagagatgaaaaacgatgagaactaacgaaggctaaaaggacaaaaaatagaaagtcgctgatgagcacccctaaatagtagaagcgaatctttttattgatttgaggataaatctcagcgaattgatgtttgagtcggacaaccaagcgaaataacaggagtccttgagtaaggaggaaaataaaaccagatagcaagagccactccaaagatgtctcgggtcttttgttaaaaaatgcgaatagtagcaaatcgataactgcaattgctgtaaaatggattctaaagagatttttcataagatgacctccctcttttatctaacttcattctactccaaaagaatgggagttacaagtaaaatgataaaaatagcagaagggagattctcttaagtcgacttgtttttttatttaagttttcttcta Mask Tandem Repeat Region ================================================ tagaagaaaacttaaataaaaaaacaagtcgacttaagagaatctcccttctgctatttttatcattttacttgtaactcccattcttttggagtagaatgaagttagataaaagagggaggtcatcttatgaaaaatctctttagaatccattttacagcaattgcagttatcgatttgctactattcgcattttttaacaaaagacccgagacatctttggagtggctcttgctatctggttttattttcctccttactcaaggactcctgttatttcgcttggttgtccgactcaaacatcaattcgctgagatttatcctcaaatcaataaaaagattcgcttctactatttaggggtgctcatcagcgactttctattttttgtccttttagccttcgttagttctcatcgtttttcatctcttatgccaatcatcactgcttgccattctactttttattatatgacagctgaccacctaagagaaaactatccagatttttacgacaaacacatctctttatgggagtgtctataaggaaaaggaggttttagc Find is-nt database================================================ Query_seq: SMT_980:SMT_981|SMT_980:SMT_981:hypothetical protein:hypothetical protein:->->:928288..928848 561 tagaagaaaacttaaataaaaaaacaagtcgacttaagagaatctcccttctgctatttttatcattttacttgtaactcccattcttttggagtagaatgaagttagataaaagagggaggtcatcttatgaaaaatctctttagaatccattttacagcaattgcagttatcgatttgctactattcgcattttttaacaaaagacccgagacatctttggagtggctcttgctatctggttttattttcctccttactcaaggactcctgttatttcgcttggttgtccgactcaaacatcaattcgctgagatttatcctcaaatcaataaaaagattcgcttctactatttaggggtgctcatcagcgactttctattttttgtccttttagccttcgttagttctcatcgtttttcatctcttatgccaatcatcactgcttgccattctactttttattatatgacagctgaccacctaagagaaaactatccagatttttacgacaaacacatctctttatgggagtgtctataaggaaaaggaggttttagc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_980:SMT_981|SMT_980:SMT_981:hypothetical protein:hypothetical protein:->->:928288..928848 561 tagaagaaaacttaaataaaaaaacaagtcgacttaagagaatctcccttctgctatttttatcattttacttgtaactcccattcttttggagtagaatgaagttagataaaagagggaggtcatcttatgaaaaatctctttagaatccattttacagcaattgcagttatcgatttgctactattcgcattttttaacaaaagacccgagacatctttggagtggctcttgctatctggttttattttcctccttactcaaggactcctgttatttcgcttggttgtccgactcaaacatcaattcgctgagatttatcctcaaatcaataaaaagattcgcttctactatttaggggtgctcatcagcgactttctattttttgtccttttagccttcgttagttctcatcgtttttcatctcttatgccaatcatcactgcttgccattctactttttattatatgacagctgaccacctaagagaaaactatccagatttttacgacaaacacatctctttatgggagtgtctataaggaaaaggaggttttagc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_980:SMT_981|SMT_980:SMT_981:hypothetical protein:hypothetical protein:->->:928288..928848 561 tagaagaaaacttaaataaaaaaacaagtcgacttaagagaatctcccttctgctatttttatcattttacttgtaactcccattcttttggagtagaatgaagttagataaaagagggaggtcatcttatgaaaaatctctttagaatccattttacagcaattgcagttatcgatttgctactattcgcattttttaacaaaagacccgagacatctttggagtggctcttgctatctggttttattttcctccttactcaaggactcctgttatttcgcttggttgtccgactcaaacatcaattcgctgagatttatcctcaaatcaataaaaagattcgcttctactatttaggggtgctcatcagcgactttctattttttgtccttttagccttcgttagttctcatcgtttttcatctcttatgccaatcatcactgcttgccattctactttttattatatgacagctgaccacctaagagaaaactatccagatttttacgacaaacacatctctttatgggagtgtctataaggaaaaggaggttttagc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 1 Min: 130 Max: 540 Len: 411 Subject: UniRef90_Q97PH6 Cluster: Conserved domain protein; n=3; Streptococcus pneumoniae|Rep: Conserved domain protein - Streptococcus pneumoniae HSP 1 e-value: 2.0E-50 bit: 200.0 Len: 411 Query Start:130 Query End:540 Subject Strand: null Subject Start: 1 Subject End: 137 ................................................................................................................................. M K N L F R I H F T A I A V I D L L L F A F F N K R P E T S L E W L L L S G X X X X X X X X X X X X X X V V R L K H Q F A E I Y P Q I N K K I R F Y Y L G V L I S D X X X X X X X X X X S S H R F S S L M P I I T A C H S T F Y Y M T A D H L R E N Y P D F Y D K H I S L W E C L ..................... ................................................................................................................................. M K K L Y R I H F I A I A V I D L L L F A F F I T R L E T S F E W L L L S G L I F F L A Q G L L L F L L V V R L K H Q F A E I Y P Q I N K K I R F Y Y L G V L T I D F L F F V L L A F I S S Q R F S S L M P I I T A C H S T F Y Y M T A D Y L R E N Y P D F Y D K H I S L W E C L ..................... tagaagaaaacttaaataaaaaaacaagtcgacttaagagaatctcccttctgctatttttatcattttacttgtaactcccattcttttggagtagaatgaagttagataaaagagggaggtcatcttnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntaaggaaaaggaggttttagc Predict ORF larger than 30AA ================================================ Protein_Len: 32 Strand: + Start: 216 End: 311 ....................................................................................................................................................................................................................... M F G V A L A I W F Y F P P Y S R T P V I S L G C P T Q T S I R .......................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: + Start: 137 End: 355 ........................................................................................................................................ M S L E S I L Q Q L Q L S I C Y Y S H F L T K D P R H L W S G S C Y L V L F S S L L K D S C Y F A W L S D S N I N S L R .............................................................................................................................................................................................................. Protein_Len: 60 Strand: + Start: 109 End: 540 ............................................................................................................ M K E G G H L M K N L F R I H F T A I A V I D L L L F A F F N K R P E T S L E W L L L S G F I F L L T Q G L L L F R L V ..................... Protein_Len: 41 Strand: - Start: 126 End: 248 ............................................................................................................................. R I F F R K L I W K V A I A T I S K S S N A N K L L L G S V D K S H S K S D P K M ......................................................................................................................................................................................................................................................................................................................... Protein_Len: 47 Strand: - Start: 37 End: 177 .................................... S F R G E A I K I M K S T V G M R K S Y F S T L Y F L S T M K H F I E K S D M K C C N C N D M ................................................................................................................................................................................................................................................................................................................................................................................................ tagaagaaaacttaaataaaaaaacaagtcgacttaagagaatctcccttctgctatttttatcattttacttgtaactcccattcttttggagtagaatgaagttagataaaagagggaggtcatcttnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntaaggaaaaggaggttttagc Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 84 Start: 71 End: 100 ......................................................................CTTGTAACTCCCATTCTTTTGGAGTAGAAT............................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ PromScan Matrix: RpoN Strand: + Score: 82 Start: 220 End: 236 ...........................................................................................................................................................................................................................TTGGAGTGGCTCTTGCT..................................................................................................................................................................................................................................................................................................................................... tagaagaaaacttaaataaaaaaacaagtcgacttaagagaatctcccttctgctatttttatcattttacttgtaactcccattcttttggagtagaatgaagttagataaaagagggaggtcatcttnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntaaggaaaaggaggttttagc Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: SMT_980:SMT_981|SMT_980:SMT_981:hypothetical protein:hypothetical protein:->->:928288..928848 561 tagaagaaaacttaaataaaaaaacaagtcgacttaagagaatctcccttctgctatttttatcattttacttgtaactcccattcttttggagtagaatgaagttagataaaagagggaggtcatcttnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntaaggaaaaggaggttttagc Intra-Species Hit: Count: 1 Min: 1 Max: 129 Len: 129 Subject: smit_SMT_980_SMT_981|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[928288,928848]|561 HSP 1 e-value: 2.0E-54 bit: 214.0 Len: 129 Query Start:1 Query End:129 Subject Strand: POSITIVE Subject Start: 1 Subject End: 129 tagaagaaaacttaaatnnnnnnncaagtcgacttaagagaatctcccttctgctatttttatcattttacttgtaactcccattcttttggagtagaatgaagttagataaaagagggaggtcatctt................................................................................................................................................................................................................................................................................................................................................................................................................................................ tagaagaaaacttaaataaaaaaacaagtcgacttaagagaatctcccttctgctatttttatcattttacttgtaactcccattcttttggagtagaatgaagttagataaaagagggaggtcatctt................................................................................................................................................................................................................................................................................................................................................................................................................................................ Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGAAGAAAACUUAAAUAAAAAAACAAGUCGACUUAAGAGAAUCUCCCUUCUGCUAUUUUUAUCAUUUUACUUGUAACUCCCAUUCUUUUGGAGUAGAAUGAAGUUAGAUAAAAGAGGGAGGUCAUCUU ...................................((((..((((((((.((..(((((...((((((((((........(((......)))))))))))))....)))))..))))))))))..)))) (-30.00) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAGAUGACCUCCCUCUUUUAUCUAACUUCAUUCUACUCCAAAAGAAUGGGAGUUACAAGUAAAAUGAUAAAAAUAGCAGAAGGGAGAUUCUCUUAAGUCGACUUGUUUUUUUAUUUAAGUUUUCUUCUA ((((.((.((((((((((((((((((((((((((........))))).)))))))..........))))))......)).)))))).)).))))(((..((((((..........))))))..)))... (-25.70) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 303 68 25 200 158 smit:SMT_959|5end_1-acyl-sn-glycerol-3-phosphate_acyltransferase_905283..905513_POSITIVE 276 80 32 59 20 smit:SMT_1333|5end_glycosyltransferase_1308635..1308865_NEGATIVE 268 89 46 64 12 smit:SMT_199|5end_K07166_ACT_domain-containing_protein_129471..129701_POSITIVE 254 88 48 38 1 smit:SMT_308|5end_trans-2-enoyl-ACP_reductase_II_249439..249669_POSITIVE 240 118 79 77 28 smit:SMT_1682|5end_K07282_poly-gamma-glutamate_synthesis_protein_(capsule_biosynthesis_protein)_1665337..1665567_POSITIVE 236 75 31 119 72 smit:SMT_723|5end_hypothetical_protein_629475..629705_NEGATIVE 233 91 53 159 122 smit:SMT_1316|5end_dTDP-glucose_4,6-dehydratase_1280894..1281124_POSITIVE 225 54 27 50 28 smit:SMT_382|5end_valyl-tRNA_synthetase_317706..317936_POSITIVE 223 120 78 75 20 smit:SMT_2033|5end_hydrolase,_haloacid_dehalogenase-like_family_1946492..1946722_POSITIVE 223 88 43 95 40 smit:SMT_2023|5end_tryptophan_synthase,_alpha_subunit_1939315..1939545_POSITIVE 223 120 71 219 177 smit:SMT_1083|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1020829..1021059_POSITIVE 221 79 44 218 190 smit:SMT_2078|5end_acetyl_xylan_esterase,_putative_1979571..1979801_POSITIVE 218 50 29 37 15 smit:SMT_962|5end_putative_hemin_transport_system_ATP-binding_protein_fecE_908584..908814_NEGATIVE 218 71 31 209 158 smit:SMT_209|5end_acetyltransferase,_GNAT_family_142673..142903_POSITIVE 215 86 47 61 4 smit:SMT_886|5end_single-stranded-DNA-specific_exonuclease_RecJ_834575..834805_NEGATIVE 211 98 60 116 85 smit:SMT_1384|5end_glutaredoxin_1362227..1362457_NEGATIVE 211 86 45 170 134 smit:SMT_705|5end_hypothetical_protein_615336..615566_NEGATIVE 209 97 51 220 161 smit:SMT_1078|5end_folylpolyglutamate_synthase_1016713..1016943_NEGATIVE 207 69 24 186 148 smit:SMT_1365|5end_translation_initiation_factor_IF-3_1344485..1344715_NEGATIVE 207 51 11 223 175 smit:SMT_854|5end_hypothetical_protein_799492..799722_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 303 68 25 76 34 smit:SMT_958|3end_hypothetical_protein_predicted_by_Glimmer/Critica_905239..905389_POSITIVE 299 68 25 87 45 smit:SMT_2024|3end_hypothetical_protein_1940690..1940840_POSITIVE 299 68 25 76 34 smit:SMT_2002|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1924145..1924295_NEGATIVE 299 68 25 87 45 smit:SMT_1981|3end_K03696_ATP-dependent_Clp_protease_ATP-binding_subunit_ClpC_1912868..1913018_POSITIVE 299 68 25 92 50 smit:SMT_1971|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1903526..1903676_POSITIVE 299 68 25 76 34 smit:SMT_1966|3end_DNA_polymerase_III_alpha_subunit_1898328..1898478_POSITIVE 299 68 25 87 45 smit:SMT_1942|3end_zinc_metalloprotease_ZmpB,_truncated_1878384..1878534_POSITIVE 299 68 25 76 34 smit:SMT_1927|3end_cell_division_protein_FtsH_1868408..1868558_NEGATIVE 299 68 25 87 45 smit:SMT_1926|3end_mevalonate_kinase_1868062..1868212_POSITIVE 299 68 25 87 45 smit:SMT_1887|3end_LicC_protein_1841740..1841890_POSITIVE 299 68 25 76 34 smit:SMT_1885|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1839830..1839980_NEGATIVE 299 68 25 76 34 smit:SMT_1873|3end_K02006_cobalt_transport_system_ATP-binding_protein_1832351..1832501_POSITIVE 299 68 25 92 50 smit:SMT_1852|3end_1,4-alpha-glucan_branching_enzyme_1816467..1816617_NEGATIVE 299 68 25 92 50 smit:SMT_326|3end_K02029_polar_amino_acid_transport_system_permease_protein_263217..263367_NEGATIVE 248 110 63 143 98 smit:SMT_76|3end_DNA_repair_and_genetic_recombination_protein_3854..4004_POSITIVE 233 91 53 137 100 smit:SMT_1315|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1280952..1281102_POSITIVE 229 109 62 122 87 smit:SMT_1637|3end_hypothetical_protein_1623848..1623998_POSITIVE 221 113 81 52 16 smit:SMT_346|3end_K01992_ABC-2_type_transport_system_permease_protein_285849..285999_POSITIVE 218 87 52 91 52 smit:SMT_1614|3end_K08998_hypothetical_protein_1601652..1601802_POSITIVE 218 50 29 35 13 smit:SMT_963|3end_K02015_iron_complex_transport_system_permease_protein_908586..908736_NEGATIVE