Origin IGS: gatgtttctcctttttatttggcttcatccgagtgacaggttggcatgcctgtaaccaagaagaagttatttgtctttacgacaatccctattctaccaataactaacttctttgtcaacagatttactttcttgttttaaagatgctataataatcatagcaagaaatctaagttatctgttttttaaaacgaggtgattatcatgcgtagattctatttccatctcccctactatctggtcatatttttcttttattggccactttatgagttgttcttgctagttgtttctgaccccctcacactcaagggactctatataaacaatcttctcttctttacacctctggtaatcttgattatatcgctactctatagctaccgtttccgtttctcactttggtggttaattggtaacggactgctcttttgctttactatcataacttttggtgagtttatactaatttacttgctaatctatgaaaccttggctctggtgggcatggcttccggtattggcatcaagcatattctacaaaaatgaaaaacaaaaaactttcacaaaatccttgaaaaatctcacaatcatgctataataatccatagagacaagtcacttagtccctttctactagagagtgcgtggttgctggaaacgcataggaagtctaaactgatactactcttgagtttttatgaaaacataaaacggtggccacgttagagccaatcagaggtgtccctcttttttgaggaacataaatgaaggtggaaccacgttacgacgtcctttttaggatgtcgctttttgtttttctagctttcacaatcctctgtccctgttttcagaatagggttaacacattcgtcaataatataagtaaggagaacatc .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 gatgttctccttacttatattattgacgaatgtgttaaccctattctgaaaacagggacagaggattgtgaaagctagaaaaacaaaaagcgacatcctaaaaaggacgtcgtaacgtggttccaccttcatttatgttcctcaaaaaagagggacacctctgattggctctaacgtggccaccgttttatgttttcataaaaactcaagagtagtatcagtttagacttcctatgcgtttccagcaaccacgcactctctagtagaaagggactaagtgacttgtctctatggattattatagcatgattgtgagatttttcaaggattttgtgaaagttttttgtttttcatttttgtagaatatgcttgatgccaataccggaagccatgcccaccagagccaaggtttcatagattagcaagtaaattagtataaactcaccaaaagttatgatagtaaagcaaaagagcagtccgttaccaattaaccaccaaagtgagaaacggaaacggtagctatagagtagcgatataatcaagattaccagaggtgtaaagaagagaagattgtttatatagagtcccttgagtgtgagggggtcagaaacaactagcaagaacaactcataaagtggccaataaaagaaaaatatgaccagatagtaggggagatggaaatagaatctacgcatgataatcacctcgttttaaaaaacagataacttagatttcttgctatgattattatagcatctttaaaacaagaaagtaaatctgttgacaaagaagttagttattggtagaatagggattgtcgtaaagacaaataacttcttcttggttacaggcatgccaacctgtcactcggatgaagccaaataaaaaggagaaacatc Mask Tandem Repeat Region ================================================ gatgtttctcctttttatttggcttcatccgagtgacaggttggcatgcctgtaaccaagaagaagttatttgtctttacgacaatccctattctaccaataactaacttctttgtcaacagatttactttcttgttttaaagatgctataataatcatagcaagaaatctaagttatctgttttttaaaacgaggtgattatcatgcgtagattctatttccatctcccctactatctggtcatatttttcttttattggccactttatgagttgttcttgctagttgtttctgaccccctcacactcaagggactctatataaacaatcttctcttctttacacctctggtaatcttgattatatcgctactctatagctaccgtttccgtttctcactttggtggttaattggtaacggactgctcttttgctttactatcataacttttggtgagtttatactaatttacttgctaatctatgaaaccttggctctggtgggcatggcttccggtattggcatcaagcatattctacaaaaatgaaaaacaaaaaactttcacaaaatccttgaaaaatctcacaatcatgctataataatccatagagacaagtcacttagtccctttctactagagagtgcgtggttgctggaaacgcataggaagtctaaactgatactactcttgagtttttatgaaaacataaaacggtggccacgttagagccaatcagaggtgtccctcttttttgaggaacataaatgaaggtggaaccacgttacgacgtcctttttaggatgtcgctttttgtttttctagctttcacaatcctctgtccctgttttcagaatagggttaacacattcgtcaataatataagtaaggagaacatc Find is-nt database================================================ Query_seq: SMT_969:SMT_970|SMT_969:SMT_970:30S ribosomal protein S15:threonyl-tRNA synthetase:<-->:915013..915911 899 gatgtttctcctttttatttggcttcatccgagtgacaggttggcatgcctgtaaccaagaagaagttatttgtctttacgacaatccctattctaccaataactaacttctttgtcaacagatttactttcttgttttaaagatgctataataatcatagcaagaaatctaagttatctgttttttaaaacgaggtgattatcatgcgtagattctatttccatctcccctactatctggtcatatttttcttttattggccactttatgagttgttcttgctagttgtttctgaccccctcacactcaagggactctatataaacaatcttctcttctttacacctctggtaatcttgattatatcgctactctatagctaccgtttccgtttctcactttggtggttaattggtaacggactgctcttttgctttactatcataacttttggtgagtttatactaatttacttgctaatctatgaaaccttggctctggtgggcatggcttccggtattggcatcaagcatattctacaaaaatgaaaaacaaaaaactttcacaaaatccttgaaaaatctcacaatcatgctataataatccatagagacaagtcacttagtccctttctactagagagtgcgtggttgctggaaacgcataggaagtctaaactgatactactcttgagtttttatgaaaacataaaacggtggccacgttagagccaatcagaggtgtccctcttttttgaggaacataaatgaaggtggaaccacgttacgacgtcctttttaggatgtcgctttttgtttttctagctttcacaatcctctgtccctgttttcagaatagggttaacacattcgtcaataatataagtaaggagaacatc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_969:SMT_970|SMT_969:SMT_970:30S ribosomal protein S15:threonyl-tRNA synthetase:<-->:915013..915911 899 gatgtttctcctttttatttggcttcatccgagtgacaggttggcatgcctgtaaccaagaagaagttatttgtctttacgacaatccctattctaccaataactaacttctttgtcaacagatttactttcttgttttaaagatgctataataatcatagcaagaaatctaagttatctgttttttaaaacgaggtgattatcatgcgtagattctatttccatctcccctactatctggtcatatttttcttttattggccactttatgagttgttcttgctagttgtttctgaccccctcacactcaagggactctatataaacaatcttctcttctttacacctctggtaatcttgattatatcgctactctatagctaccgtttccgtttctcactttggtggttaattggtaacggactgctcttttgctttactatcataacttttggtgagtttatactaatttacttgctaatctatgaaaccttggctctggtgggcatggcttccggtattggcatcaagcatattctacaaaaatgaaaaacaaaaaactttcacaaaatccttgaaaaatctcacaatcatgctataataatccatagagacaagtcacttagtccctttctactagagagtgcgtggttgctggaaacgcataggaagtctaaactgatactactcttgagtttttatgaaaacataaaacggtggccacgttagagccaatcagaggtgtccctcttttttgaggaacataaatgaaggtggaaccacgttacgacgtcctttttaggatgtcgctttttgtttttctagctttcacaatcctctgtccctgttttcagaatagggttaacacattcgtcaataatataagtaaggagaacatc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_969:SMT_970|SMT_969:SMT_970:30S ribosomal protein S15:threonyl-tRNA synthetase:<-->:915013..915911 899 gatgtttctcctttttatttggcttcatccgagtgacaggttggcatgcctgtaaccaagaagaagttatttgtctttacgacaatccctattctaccaataactaacttctttgtcaacagatttactttcttgttttaaagatgctataataatcatagcaagaaatctaagttatctgttttttaaaacgaggtgattatcatgcgtagattctatttccatctcccctactatctggtcatatttttcttttattggccactttatgagttgttcttgctagttgtttctgaccccctcacactcaagggactctatataaacaatcttctcttctttacacctctggtaatcttgattatatcgctactctatagctaccgtttccgtttctcactttggtggttaattggtaacggactgctcttttgctttactatcataacttttggtgagtttatactaatttacttgctaatctatgaaaccttggctctggtgggcatggcttccggtattggcatcaagcatattctacaaaaatgaaaaacaaaaaactttcacaaaatccttgaaaaatctcacaatcatgctataataatccatagagacaagtcacttagtccctttctactagagagtgcgtggttgctggaaacgcataggaagtctaaactgatactactcttgagtttttatgaaaacataaaacggtggccacgttagagccaatcagaggtgtccctcttttttgaggaacataaatgaaggtggaaccacgttacgacgtcctttttaggatgtcgctttttgtttttctagctttcacaatcctctgtccctgttttcagaatagggttaacacattcgtcaataatataagtaaggagaacatc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 1 Min: 196 Max: 402 Len: 207 Subject: UniRef90_Q97PI7 Cluster: Hypothetical protein; n=2; Streptococcus pneumoniae|Rep: Hypothetical protein - Streptococcus pneumoniae HSP 1 e-value: 3.0E-24 bit: 115.0 Len: 207 Query Start:196 Query End:402 Subject Strand: null Subject Start: 1 Subject End: 69 ................................................................................................................................................................................................... V I I M R R F Y F H L P Y Y L V I F F F Y W P L Y E L F L L V V S D P L T L K G L Y I N N L L F F T P L V X X X X X X X X X X R F R F S L ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. ................................................................................................................................................................................................... M I I M R R F Y S H L P Y Y L V I L F F Y W P L Y E L F L L V V S D P L T L K G L Y I N N L L F F T P L V I L I V S L L Y S Y R F R F S L ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. gatgtttctcctttttatttggcttcatccgagtgacaggttggcatgcctgtaaccaagaagaagttatttgtctttacgacaatccctattctaccaataactaacttctttgtcaacagatttactttcttgttttaaagatgctataataatcatagcaagaaatctaagttatctgttttttaaaacgagnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntggtggttaattggtaacggactgctcttttgctttactatcataacttttggtgagtttatactaatttacttgctaatctatgaaaccttggctctggtgggcatggcttccggtattggcatcaagcatattctacaaaaatgaaaaacaaaaaactttcacaaaatccttgaaaaatctcacaatcatgctataataatccatagagacaagtcacttagtccctttctactagagagtgcgtggttgctggaaacgcataggaagtctaaactgatactactcttgagtttttatgaaaacataaaacggtggccacgttagagccaatcagaggtgtccctcttttttgaggaacataaatgaaggtggaaccacgttacgacgtcctttttaggatgtcgctttttgtttttctagctttcacaatcctctgtccctgttttcagaatagggttaacacattcgtcaataatataagtaaggagaacatc Predict ORF larger than 30AA ================================================ Protein_Len: 31 Strand: + Start: 46 End: 138 ............................................. M P V T K K K L F V F T T I P I L P I T N F F V N R F T F L F ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: + Start: 196 End: 546 ................................................................................................................................................................................................... M I I M R R F Y F H L P Y Y L V I F F F Y W P L Y E L F L L V V S D P L T L K G L Y I N N L L F F T P L V I L I I S L L ................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 38 Strand: + Start: 485 End: 598 .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M K P W L W W A W L P V L A S S I F Y K N E K Q K T F T K S L K N L T I M L ............................................................................................................................................................................................................................................................................................................. Protein_Len: 52 Strand: + Start: 571 End: 726 .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M L E K S H N H A I I I H R D K S L S P F L L E S A W L L E T H R K S K L I L L L S F Y E N I K R W P R ............................................................................................................................................................................. Protein_Len: 44 Strand: + Start: 768 End: 899 ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M K V E P R Y D V L F R M S L F V F L A F T I L C P C F Q N R V N T F V N N I S K E N I Protein_Len: 31 Strand: - Start: 677 End: 769 .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... V S V V R S N K H F C L V T A V N S G I L P T G R K Q P V Y M .................................................................................................................................. Protein_Len: 30 Strand: - Start: 594 End: 683 ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. A I I I W L S L D S L G K R S S L A H N S S V C L F D L S M ........................................................................................................................................................................................................................ Protein_Len: 31 Strand: - Start: 178 End: 270 ................................................................................................................................................................................. R N K L V L H N D H T S E I E M E G V I Q D Y K E K I P W K M ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 35 Strand: - Start: 18 End: 122 ................. K A E D S H C T P M G T V L F F N N T K V V I G I R G I V L K K T L M ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... gatgtttctcctttttatttggcttcatccgagtgacaggttggcatgcctgtaaccaagaagaagttatttgtctttacgacaatccctattctaccaataactaacttctttgtcaacagatttactttcttgttttaaagatgctataataatcatagcaagaaatctaagttatctgttttttaaaacgagnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntggtggttaattggtaacggactgctcttttgctttactatcataacttttggtgagtttatactaatttacttgctaatctatgaaaccttggctctggtgggcatggcttccggtattggcatcaagcatattctacaaaaatgaaaaacaaaaaactttcacaaaatccttgaaaaatctcacaatcatgctataataatccatagagacaagtcacttagtccctttctactagagagtgcgtggttgctggaaacgcataggaagtctaaactgatactactcttgagtttttatgaaaacataaaacggtggccacgttagagccaatcagaggtgtccctcttttttgaggaacataaatgaaggtggaaccacgttacgacgtcctttttaggatgtcgctttttgtttttctagctttcacaatcctctgtccctgttttcagaatagggttaacacattcgtcaataatataagtaaggagaacatc Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: - Score: 93 Start: 91 End: 120 ..........................................................................................GTTGACAAAGAAGTTAGTTATTGGTAGAAT........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ gatgtttctcctttttatttggcttcatccgagtgacaggttggcatgcctgtaaccaagaagaagttatttgtctttacgacaatccctattctaccaataactaacttctttgtcaacagatttactttcttgttttaaagatgctataataatcatagcaagaaatctaagttatctgttttttaaaacgagnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntggtggttaattggtaacggactgctcttttgctttactatcataacttttggtgagtttatactaatttacttgctaatctatgaaaccttggctctggtgggcatggcttccggtattggcatcaagcatattctacaaaaatgaaaaacaaaaaactttcacaaaatccttgaaaaatctcacaatcatgctataataatccatagagacaagtcacttagtccctttctactagagagtgcgtggttgctggaaacgcataggaagtctaaactgatactactcttgagtttttatgaaaacataaaacggtggccacgttagagccaatcagaggtgtccctcttttttgaggaacataaatgaaggtggaaccacgttacgacgtcctttttaggatgtcgctttttgtttttctagctttcacaatcctctgtccctgttttcagaatagggttaacacattcgtcaataatataagtaaggagaacatc Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 79 HP_score: -5.8 Tail_Score: -5.00884 Start: 739 End: 750 Full_Region: CGTTAGAGCCAATCA GAGG TGTC CCTC TTTTTTGAGGAACAT ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................GAGGTGTCCCTC..................................................................................................................................................... TransTerm Strand: + Conf: 100 HP_score: -7.4 Tail_Score: -5.69262 Start: 787 End: 810 Full_Region: AGGTGGAACCACGTT ACGACGTCCT TTTT AGGATGTCGC TTTTTGTTTTTCTAG ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ACGACGTCCTTTTTAGGATGTCGC......................................................................................... TransTerm Strand: + Conf: 86 HP_score: -10.9 Tail_Score: -3.99117 Start: 788 End: 809 Full_Region: GGTGGAACCACGTTA CGACGTCCT TTTT AGGATGTCG CTTTTTGTTTTTCTA ...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................CGACGTCCTTTTTAGGATGTCG.......................................................................................... Find igs database================================================ Query_seq: SMT_969:SMT_970|SMT_969:SMT_970:30S ribosomal protein S15:threonyl-tRNA synthetase:<-->:915013..915911 899 gatgtttctcctttttatttggcttcatccgagtgacaggttggcatgcctgtaaccaagaagaagttatttgtctttacgacaatccctattctaccaataactaacttctttgtcaacagatttactttcttgttttaaagatgctataataatcatagcaagaaatctaagttatctgttttttaaaacgagnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntggtggttaattggtaacggactgctcttttgctttactatcataacttttggtgagtttatactaatttacttgctaatctatgaaaccttggctctggtgggcatggcttccggtattggcatcaagcatattctacaaaaatgaaaaacaaaaaactttcacaaaatccttgaaaaatctcacaatcatgctataataatccatagagacaagtcacttagtccctttctactagagagtgcgtggttgctggaaacgcataggaagtctaaactgatactactcttgagtttttatgaaaacataaaacggtggccacgttagagccaatcagaggtgtccctcttttttgaggaacataaatgaaggtggaaccacgttacgacgtcctttttaggatgtcgctttttgtttttctagctttcacaatcctctgtccctgttttcagaatagggttaacacattcgtcaataatataagtaaggagaacatc Intra-Species Hit: Count: 1 Min: 1 Max: 899 Len: 899 Subject: smit_SMT_969_SMT_970|30S ribosomal protein S15:threonyl-tRNA synthetase|NEGATIVE:POSITIVE|[915013,915911]|899 HSP 1 e-value: 1.0E-179 bit: 628.0 Len: 317 Query Start:583 Query End:899 Subject Strand: POSITIVE Subject Start: 583 Subject End: 899 ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tctcacaatcatgctataataatccatagagacaagtcacttagtccctttctactagagagtgcgtggttgctggaaacgcataggaagtctaaactgatactactcttgagtttttatgaaaacataaaacggtggccacgttagagccaatcagaggtgtccctcttttttgaggaacataaatgaaggtggaaccacgttacgacgtcctttttaggatgtcgctttttgtttttctagctttcacaatcctctgtccctgttttcagaatagggttaacacattcgtcaataatataagtaaggagaacatc ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tctcacaatcatgctataataatccatagagacaagtcacttagtccctttctactagagagtgcgtggttgctggaaacgcataggaagtctaaactgatactactcttgagtttttatgaaaacataaaacggtggccacgttagagccaatcagaggtgtccctcttttttgaggaacataaatgaaggtggaaccacgttacgacgtcctttttaggatgtcgctttttgtttttctagctttcacaatcctctgtccctgttttcagaatagggttaacacattcgtcaataatataagtaaggagaacatc HSP 2 e-value: 1.0E-105 bit: 385.0 Len: 194 Query Start:1 Query End:194 Subject Strand: POSITIVE Subject Start: 1 Subject End: 194 gatgtttctcctttttatttggcttcatccgagtgacaggttggcatgcctgtaaccaagaagaagttatttgtctttacgacaatccctattctaccaataactaacttctttgtcaacagatttactttcttgttttaaagatgctataataatcatagcaagaaatctaagttatctgttttttaaaacga................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. gatgtttctcctttttatttggcttcatccgagtgacaggttggcatgcctgtaaccaagaagaagttatttgtctttacgacaatccctattctaccaataactaacttctttgtcaacagatttactttcttgttttaaagatgctataataatcatagcaagaaatctaagttatctgttttttaaaacga................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. HSP 3 e-value: 1.0E-72 bit: 276.0 Len: 139 Query Start:403 Query End:541 Subject Strand: POSITIVE Subject Start: 403 Subject End: 541 ..................................................................................................................................................................................................................................................................................................................................................................................................................tggtggttaattggtaacggactgctcttttgctttactatcataacttttggtgagtttatactaatttacttgctaatctatgaaaccttggctctggtgggcatggcttccggtattggcatcaagcatattctac...................................................................................................................................................................................................................................................................................................................................................................... ..................................................................................................................................................................................................................................................................................................................................................................................................................tggtggttaattggtaacggactgctcttttgctttactatcataacttttggtgagtttatactaatttacttgctaatctatgaaaccttggctctggtgggcatggcttccggtattggcatcaagcatattctac...................................................................................................................................................................................................................................................................................................................................................................... Inter-species Hit: Count: 7 Min: 1 Max: 814 Len: 814 Subject: NC_008533_SPD_1441_SPD_1442|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[1458345,1458962]|618 HSP 1 e-value: 1.0E-88 bit: 329.0 Len: 185 Query Start:583 Query End:767 Subject Strand: POSITIVE Subject Start: 433 Subject End: 618 ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tctcacaatcatgctataataatccatagagacaagtcacttagtccctttctactagagagtgcgtggttgctggaaacgcataggaagtctaaactgatactactcttgag-tttttatgaaaacataaaacggtggccacgttagagccaatcagaggtgtccctcttttttgaggaacataa.................................................................................................................................... ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tctcacaatcatgctataataatccatagagacaagtcacttagtccctttctactagagagtgcgtggttgctggaaacgcataggaagtctaaactgatactactcttgagttttttatgaaaacataaaacggtggccacgttagagccgatcagaggtgtccctctcttttgaggtacataa.................................................................................................................................... HSP 2 e-value: 6.0E-47 bit: 190.0 Len: 136 Query Start:406 Query End:541 Subject Strand: POSITIVE Subject Start: 255 Subject End: 390 .....................................................................................................................................................................................................................................................................................................................................................................................................................tggttaattggtaacggactgctcttttgctttactatcataacttttggtgagtttatactaatttacttgctaatctatgaaaccttggctctggtgggcatggcttccggtattggcatcaagcatattctac...................................................................................................................................................................................................................................................................................................................................................................... .....................................................................................................................................................................................................................................................................................................................................................................................................................tggttagttggtaacggactgctcttttactttactatcataacctttggtgagtttatactaatttacttgctaatctatgaaacagttgctctggtcggcatggattctggtattagcatcaagcatattctac...................................................................................................................................................................................................................................................................................................................................................................... HSP 3 e-value: 9.0E-6 bit: 54.0 Len: 35 Query Start:153 Query End:187 Subject Strand: POSITIVE Subject Start: 1 Subject End: 35 ........................................................................................................................................................taatcatagcaagaaatctaagttatctgtttttt........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ ........................................................................................................................................................taatcatagcaagagatctaagttgtctgtttttt........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ Subject: NC_003098_spr1469_spr1470|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[1451068,1451431]|364 HSP 1 e-value: 1.0E-88 bit: 329.0 Len: 185 Query Start:583 Query End:767 Subject Strand: POSITIVE Subject Start: 179 Subject End: 364 ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tctcacaatcatgctataataatccatagagacaagtcacttagtccctttctactagagagtgcgtggttgctggaaacgcataggaagtctaaactgatactactcttgag-tttttatgaaaacataaaacggtggccacgttagagccaatcagaggtgtccctcttttttgaggaacataa.................................................................................................................................... ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tctcacaatcatgctataataatccatagagacaagtcacttagtccctttctactagagagtgcgtggttgctggaaacgcataggaagtctaaactgatactactcttgagttttttatgaaaacataaaacggtggccacgttagagccgatcagaggtgtccctctcttttgaggtacataa.................................................................................................................................... HSP 2 e-value: 6.0E-47 bit: 190.0 Len: 136 Query Start:406 Query End:541 Subject Strand: POSITIVE Subject Start: 1 Subject End: 136 .....................................................................................................................................................................................................................................................................................................................................................................................................................tggttaattggtaacggactgctcttttgctttactatcataacttttggtgagtttatactaatttacttgctaatctatgaaaccttggctctggtgggcatggcttccggtattggcatcaagcatattctac...................................................................................................................................................................................................................................................................................................................................................................... .....................................................................................................................................................................................................................................................................................................................................................................................................................tggttagttggtaacggactgctcttttactttactatcataacctttggtgagtttatactaatttacttgctaatctatgaaacagttgctctggtcggcatggattctggtattagcatcaagcatattctac...................................................................................................................................................................................................................................................................................................................................................................... Subject: NC_003028_SP_1629_SP_1630|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[1529919,1530108]|190 HSP 1 e-value: 1.0E-88 bit: 329.0 Len: 185 Query Start:583 Query End:767 Subject Strand: POSITIVE Subject Start: 5 Subject End: 190 ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tctcacaatcatgctataataatccatagagacaagtcacttagtccctttctactagagagtgcgtggttgctggaaacgcataggaagtctaaactgatactactcttgag-tttttatgaaaacataaaacggtggccacgttagagccaatcagaggtgtccctcttttttgaggaacataa.................................................................................................................................... ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tctcacaatcatgctataataatccatagagacaagtcacttagtccctttctactagagagtgcgtggttgctggaaacgcataggaagtctaaactgatactactcttgagttttttatgaaaacataaaacggtggccacgttagagccgatcagaggtgtccctctcttttgaggtacataa.................................................................................................................................... Subject: NC_008533_SPD_1439_SPD_1440|ribosomal protein S15:hypothetical protein|NEGATIVE:NEGATIVE|[1457390,1457529]|140 HSP 1 e-value: 6.0E-47 bit: 190.0 Len: 100 Query Start:1 Query End:100 Subject Strand: POSITIVE Subject Start: 1 Subject End: 100 gatgtttctcctttttatttggcttcatccgagtgacaggttggcatgcctgtaaccaagaagaagttatttgtctttacgacaatccctattctaccaa............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... gatgtttctcctttttatttggcttcatccgagtgacaggttggcatgcctgtaaccaagaagaagttatttgtctttacgacaatccctattttaccaa............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Subject: NC_003098_spr1467_spr1468|30S ribosomal protein S15:hypothetical protein|NEGATIVE:NEGATIVE|[1449859,1449998]|140 HSP 1 e-value: 6.0E-47 bit: 190.0 Len: 100 Query Start:1 Query End:100 Subject Strand: POSITIVE Subject Start: 1 Subject End: 100 gatgtttctcctttttatttggcttcatccgagtgacaggttggcatgcctgtaaccaagaagaagttatttgtctttacgacaatccctattctaccaa............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... gatgtttctcctttttatttggcttcatccgagtgacaggttggcatgcctgtaaccaagaagaagttatttgtctttacgacaatccctattttaccaa............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Subject: NC_003028_SP_1626_SP_1627|30S ribosomal protein S15:hypothetical protein|NEGATIVE:NEGATIVE|[1528538,1528677]|140 HSP 1 e-value: 6.0E-47 bit: 190.0 Len: 100 Query Start:1 Query End:100 Subject Strand: POSITIVE Subject Start: 1 Subject End: 100 gatgtttctcctttttatttggcttcatccgagtgacaggttggcatgcctgtaaccaagaagaagttatttgtctttacgacaatccctattctaccaa............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... gatgtttctcctttttatttggcttcatccgagtgacaggttggcatgcctgtaaccaagaagaagttatttgtctttacgacaatccctattttaccaa............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Subject: NC_004350_SMU.1587c_SMU.1588c|hypothetical protein:putative hexosyltransferase|NEGATIVE:NEGATIVE|[1518218, 1518481]|264 HSP 1 e-value: 3.0E-18 bit: 95.6 Len: 119 Query Start:696 Query End:814 Subject Strand: NEGATIVE Subject Start: 22 Subject End: 138 .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tttttatgaaaacataaaacggtggccacgttagagccaatcagaggtgtccctcttttttgaggaacataaatgaaggtggaaccacgttacgacgtcctttttaggatgtcgctttt..................................................................................... .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tttttatgtaaacataaaacggtggctacgttagagccgattcgaggtgtgagtctttt--gacttgcataaatgaaggtggaaccacgttgcgacgtccttttaaggatgtcgttttt..................................................................................... Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GAUGUUUCUCCUUUUUAUUUGGCUUCAUCCGAGUGACAGGUUGGCAUGCCUGUAACCAAGAAGAAGUUAUUUGUCUUUACGACAAUCCCUAUUCUACCAAUAACUAACUUCUUUGUCAACAGAUUUACUUUCUUGUUUUAAAGAUGCUAUAAUAAUCAUAGCAAGAAAUCUAAGUUAUCUGUUUUUUAAAACGAGGUGAUUAUCAUGCGUAGAUUCUAUUUCCAUCUCCCCUACUAUCUGGUCAUAUUUUUCUUUUAUUGGCCACUUUAUGAGUUGUUCUUGCUAGUUGUUUCUGACCCCCUCACACUCAAGGGACUCUAUAUAAACAAUCUUCUCUUCUUUACACCUCUGGUAAUCUUGAUUAUAUCGCUACUCUAUAGCUACCGUUUCCGUUUCUCACUUUGGUGGUUAAUUGGUAACGGACUGCUCUUUUGCUUUACUAUCAUAACUUUUGGUGAGUUUAUACUAAUUUACUUGCUAAUCUAUGAAACCUUGGCUCUGGUGGGCAUGGCUUCCGGUAUUGGCAUCAAGCAUAUUCUACAAAAAUGAAAAACAAAAAACUUUCACAAAAUCCUUGAAAAAUCUCACAAUCAUGCUAUAAUAAUCCAUAGAGACAAGUCACUUAGUCCCUUUCUACUAGAGAGUGCGUGGUUGCUGGAAACGCAUAGGAAGUCUAAACUGAUACUACUCUUGAGUUUUUAUGAAAACAUAAAACGGUGGCCACGUUAGAGCCAAUCAGAGGUGUCCCUCUUUUUUGAGGAACAUAAAUGAAGGUGGAACCACGUUACGACGUCCUUUUUAGGAUGUCGCUUUU (((((...(((..(((((.(((..((....(((((((((((......))))))......((((((((((.(((((.....)))))....((((.....))))..)))))))))).....((((.......((((......))))......((((((..(((((((((.....((((((((((((....)))).)))))))).(((((.((((..................))))....((((((..............))))))...)))))....))))))))))))))))))).........)))))....))..))).)))))..((((((........(((((((((((......(((...)))....(((((..((((((((((...((((..((((.((((((..((..((...(((((..((((.(((.......(((....(((((..((((((.............(((((((((..(((.((...((((....))))..)).))).)).))))))).........(((.........))).......))))))...)))))....)))....(((..(((((((((................((((((...))).))).(((((.....))))).)))))))))..)))...))).)))))))))..))..))))))))...))))......)))).....)))))))))).....)))))...))))))))))).((((......)))).........)))))))))...))))).(((((((((....)))))))))..... (-195.01) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAAAGCGACAUCCUAAAAAGGACGUCGUAACGUGGUUCCACCUUCAUUUAUGUUCCUCAAAAAAGAGGGACACCUCUGAUUGGCUCUAACGUGGCCACCGUUUUAUGUUUUCAUAAAAACUCAAGAGUAGUAUCAGUUUAGACUUCCUAUGCGUUUCCAGCAACCACGCACUCUCUAGUAGAAAGGGACUAAGUGACUUGUCUCUAUGGAUUAUUAUAGCAUGAUUGUGAGAUUUUUCAAGGAUUUUGUGAAAGUUUUUUGUUUUUCAUUUUUGUAGAAUAUGCUUGAUGCCAAUACCGGAAGCCAUGCCCACCAGAGCCAAGGUUUCAUAGAUUAGCAAGUAAAUUAGUAUAAACUCACCAAAAGUUAUGAUAGUAAAGCAAAAGAGCAGUCCGUUACCAAUUAACCACCAAAGUGAGAAACGGAAACGGUAGCUAUAGAGUAGCGAUAUAAUCAAGAUUACCAGAGGUGUAAAGAAGAGAAGAUUGUUUAUAUAGAGUCCCUUGAGUGUGAGGGGGUCAGAAACAACUAGCAAGAACAACUCAUAAAGUGGCCAAUAAAAGAAAAAUAUGACCAGAUAGUAGGGGAGAUGGAAAUAGAAUCUACGCAUGAUAAUCACCUCGUUUUAAAAAACAGAUAACUUAGAUUUCUUGCUAUGAUUAUUAUAGCAUCUUUAAAACAAGAAAGUAAAUCUGUUGACAAAGAAGUUAGUUAUUGGUAGAAUAGGGAUUGUCGUAAAGACAAAUAACUUCUUCUUGGUUACAGGCAUGCCAACCUGUCACUCGGAUGAAGCCAAAUAAAAAGGAGAAACAUC ....(((((.((((....)))).)))))..((((((((((.((((.....((((((((......))))))))((((((.((((((((....(((((((..(((((((....)))))))......((((.((.((.((.(((.....))).))(((((..((..((.(((((((.........((((((((..((((((((.(((((((((((((((((((......(((((.....(((((.((((((..(((((............)))))..))))))...)))))(((.(((....((((((..((.(....).))...))))))....))).)))................))))).....)))))))))))...((......))...(((((.((.......(((....)))......)).)))))...))))))))............)))).)))).....(((((((.((.......)).)))))))..))))))))))))))).))..))..))))).........)))).)))).....))))))).....))).......).)))).))).))))))).))))).......))))).(((((((((.(((.((((((.......(((((((..(((((((((((((((...)))))))........((((.((.......))))))....)))))))))))))))..))))))))))))))))))............((((.(((.(((.(((((........))))).))).))).))))...................... (-209.26) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 967 70 21 161 112 smit:SMT_969|5end_30S_ribosomal_protein_S15_914922..915152_NEGATIVE 385 540 491 202 139 smit:SMT_568|5end_hypothetical_protein_485674..485904_POSITIVE 343 537 494 206 151 smit:SMT_992|5end_hypothetical_protein_934882..935112_NEGATIVE 341 758 715 83 26 smit:SMT_1376|5end_tetrapyrrole_(corrin/porphyrin)_methylase_family_protein_1355553..1355783_NEGATIVE 336 49 5 55 4 smit:SMT_2164|5end_K06147_ATP-binding_cassette,_subfamily_B,_bacterial_2037861..2038091_POSITIVE 331 758 711 64 2 smit:SMT_354|5end_hypothetical_protein_291343..291573_POSITIVE 329 526 486 59 3 smit:SMT_2000|5end_hypothetical_protein_1923108..1923338_NEGATIVE 329 529 490 59 3 smit:SMT_847|5end_thioredoxin_reductase_792800..793030_POSITIVE 326 50 5 154 97 smit:SMT_1857|5end_hypothetical_protein_1820931..1821161_POSITIVE 326 50 5 154 97 smit:SMT_834|5end_hypothetical_protein_777112..777342_NEGATIVE 326 760 717 111 65 smit:SMT_712|5end_alcohol_dehydrogenase,_zinc-containing_620381..620611_NEGATIVE 319 530 495 200 164 smit:SMT_767|5end_hypothetical_protein_676454..676684_NEGATIVE 315 657 619 138 98 smit:SMT_522|5end_K02003_446719..446949_POSITIVE 312 258 211 124 78 smit:SMT_1728|5end_exodeoxyribonuclease_VII_large_subunit_1713248..1713478_POSITIVE 310 543 504 72 38 smit:SMT_924|5end_primosomal_protein_N'_879903..880133_NEGATIVE 310 659 616 47 10 smit:SMT_897|5end_serine_O-acetyltransferase_844632..844862_NEGATIVE 309 58 17 209 169 smit:SMT_1103|5end_hypothetical_protein_1045744..1045974_NEGATIVE 307 524 488 49 13 smit:SMT_1554|5end_isoleucyl-tRNA_synthetase_1537040..1537270_POSITIVE 306 259 211 224 174 smit:SMT_2024|5end_hypothetical_protein_1940359..1940589_POSITIVE 306 265 221 122 56 smit:SMT_1644|5end_3-isopropylmalate_dehydrogenase_1630704..1630934_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 416 532 496 53 9 smit:SMT_1946|3end_sucrose-6-phosphate_hydrolase_1881205..1881355_POSITIVE 346 755 711 92 34 smit:SMT_2045|3end_K+_transport_system,_NAD-binding_component_1956758..1956908_POSITIVE 341 758 715 80 23 smit:SMT_1377|3end_hypothetical_protein_1355556..1355706_NEGATIVE 332 533 493 108 66 smit:SMT_2005|3end_pullulanase,_type_I_1925615..1925765_NEGATIVE 326 50 5 142 85 smit:SMT_1856|3end_polypeptide_deformylase_1820999..1821149_POSITIVE 326 50 5 142 85 smit:SMT_835|3end_polypeptide_deformylase_777124..777274_NEGATIVE 321 520 474 85 31 smit:SMT_1941|3end_hypothetical_protein_1877663..1877813_POSITIVE 306 265 221 110 44 smit:SMT_1643|3end_2-isopropylmalate_synthase_1630772..1630922_POSITIVE 306 538 496 83 37 smit:SMT_1557|3end_hypothetical_protein_1543650..1543800_POSITIVE 300 527 486 70 21 smit:SMT_632|3end_hypothetical_protein_537274..537424_NEGATIVE 296 537 491 58 10 smit:SMT_826|3end_hypothetical_protein_749591..749741_NEGATIVE 296 64 21 57 21 smit:SMT_399|3end_cytochrome_c-type_biogenesis_protein_CcdA_329156..329306_POSITIVE 294 517 476 60 19 smit:SMT_2075|3end_phosphoribosylamine--glycine_ligase_1977769..1977919_NEGATIVE 294 54 19 46 10 smit:SMT_2024|3end_hypothetical_protein_1940690..1940840_POSITIVE 294 54 19 46 10 smit:SMT_1981|3end_K03696_ATP-dependent_Clp_protease_ATP-binding_subunit_ClpC_1912868..1913018_POSITIVE 294 54 19 51 15 smit:SMT_1971|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1903526..1903676_POSITIVE 294 54 19 46 10 smit:SMT_1942|3end_zinc_metalloprotease_ZmpB,_truncated_1878384..1878534_POSITIVE 294 54 19 46 10 smit:SMT_1926|3end_mevalonate_kinase_1868062..1868212_POSITIVE 294 54 19 46 10 smit:SMT_1887|3end_LicC_protein_1841740..1841890_POSITIVE 294 54 19 51 15 smit:SMT_1852|3end_1,4-alpha-glucan_branching_enzyme_1816467..1816617_NEGATIVE