Origin IGS: ttacttcctcctaggtgagcgagcacttacttttttaaagataaatctattatagtgagtgaatactcacttgtcaagtgatgatttgaatttttttaaaacaaaaaatctccccgtagggagaatatctggtttattttaccttacagtgctaggaaccgtaacctaagattatacttgagcataccaaggtaaggtgacaacgtaaaaagccctctgaaaatcagagagctccagtcttgctggataaaccgtttatagactaggcgacgaggcgaagattgtacaaaatttttagtgaaacaaaaaaatctccccgtagggagaatatctggtttattttaccttacagtgctaggaaccgtaaccaaagataatacttgagcatatcaaggtaaggtgacaacgtaaaaagccctctgaaaatcagagagctacagtcttgctggataaaccgtttatagactaggcgacgaggcgaagattgtacaaaatttttagtgaaacaaaaaaatctccccgaagggagatcattctggtttattttaccttacagtgctaggaaccgtaaccgaagataatacttgagtatatcgaggtaaggtgacaacgcaatgtaagtggaaaataaagcaga .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tctgctttattttccacttacattgcgttgtcaccttacctcgatatactcaagtattatcttcggttacggttcctagcactgtaaggtaaaataaaccagaatgatctcccttcggggagatttttttgtttcactaaaaattttgtacaatcttcgcctcgtcgcctagtctataaacggtttatccagcaagactgtagctctctgattttcagagggctttttacgttgtcaccttaccttgatatgctcaagtattatctttggttacggttcctagcactgtaaggtaaaataaaccagatattctccctacggggagatttttttgtttcactaaaaattttgtacaatcttcgcctcgtcgcctagtctataaacggtttatccagcaagactggagctctctgattttcagagggctttttacgttgtcaccttaccttggtatgctcaagtataatcttaggttacggttcctagcactgtaaggtaaaataaaccagatattctccctacggggagattttttgttttaaaaaaattcaaatcatcacttgacaagtgagtattcactcactataatagatttatctttaaaaaagtaagtgctcgctcacctaggaggaagtaa Mask Tandem Repeat Region ================================================ ttacttcctcctaggtgagcgagcacttacttttttaaagataaatctattatagtgagtgaatactcacttgtcaagtgatgatttgaatttttttannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnncaatgtaagtggaaaataaagcaga Find is-nt database================================================ Query_seq: SMT_968:SMT_969|SMT_968:SMT_969:K01990 ABC-2 type transport system ATP-binding protein:30S ribosomal protein S15:<-<-:914106..914742 637 ttacttcctcctaggtgagcgagcacttacttttttaaagataaatctattatagtgagtgaatactcacttgtcaagtgatgatttgaatttttttaaaacaaaaaatctccccgtagggagaatatctggtttattttaccttacagtgctaggaaccgtaacctaagattatacttgagcataccaaggtaaggtgacaacgtaaaaagccctctgaaaatcagagagctccagtcttgctggataaaccgtttatagactaggcgacgaggcgaagattgtacaaaatttttagtgaaacaaaaaaatctccccgtagggagaatatctggtttattttaccttacagtgctaggaaccgtaaccaaagataatacttgagcatatcaaggtaaggtgacaacgtaaaaagccctctgaaaatcagagagctacagtcttgctggataaaccgtttatagactaggcgacgaggcgaagattgtacaaaatttttagtgaaacaaaaaaatctccccgaagggagatcattctggtttattttaccttacagtgctaggaaccgtaaccgaagataatacttgagtatatcgaggtaaggtgacaacgcaatgtaagtggaaaataaagcaga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_968:SMT_969|SMT_968:SMT_969:K01990 ABC-2 type transport system ATP-binding protein:30S ribosomal protein S15:<-<-:914106..914742 637 ttacttcctcctaggtgagcgagcacttacttttttaaagataaatctattatagtgagtgaatactcacttgtcaagtgatgatttgaatttttttaaaacaaaaaatctccccgtagggagaatatctggtttattttaccttacagtgctaggaaccgtaacctaagattatacttgagcataccaaggtaaggtgacaacgtaaaaagccctctgaaaatcagagagctccagtcttgctggataaaccgtttatagactaggcgacgaggcgaagattgtacaaaatttttagtgaaacaaaaaaatctccccgtagggagaatatctggtttattttaccttacagtgctaggaaccgtaaccaaagataatacttgagcatatcaaggtaaggtgacaacgtaaaaagccctctgaaaatcagagagctacagtcttgctggataaaccgtttatagactaggcgacgaggcgaagattgtacaaaatttttagtgaaacaaaaaaatctccccgaagggagatcattctggtttattttaccttacagtgctaggaaccgtaaccgaagataatacttgagtatatcgaggtaaggtgacaacgcaatgtaagtggaaaataaagcaga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_968:SMT_969|SMT_968:SMT_969:K01990 ABC-2 type transport system ATP-binding protein:30S ribosomal protein S15:<-<-:914106..914742 637 ttacttcctcctaggtgagcgagcacttacttttttaaagataaatctattatagtgagtgaatactcacttgtcaagtgatgatttgaatttttttaaaacaaaaaatctccccgtagggagaatatctggtttattttaccttacagtgctaggaaccgtaacctaagattatacttgagcataccaaggtaaggtgacaacgtaaaaagccctctgaaaatcagagagctccagtcttgctggataaaccgtttatagactaggcgacgaggcgaagattgtacaaaatttttagtgaaacaaaaaaatctccccgtagggagaatatctggtttattttaccttacagtgctaggaaccgtaaccaaagataatacttgagcatatcaaggtaaggtgacaacgtaaaaagccctctgaaaatcagagagctacagtcttgctggataaaccgtttatagactaggcgacgaggcgaagattgtacaaaatttttagtgaaacaaaaaaatctccccgaagggagatcattctggtttattttaccttacagtgctaggaaccgtaaccgaagataatacttgagtatatcgaggtaaggtgacaacgcaatgtaagtggaaaataaagcaga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ttacttcctcctaggtgagcgagcacttacttttttaaagataaatctattatagtgagtgaatactcacttgtcaagtgatgatttgaatttttttaaaacaaaaaatctccccgtagggagaatatctggtttattttaccttacagtgctaggaaccgtaacctaagattatacttgagcataccaaggtaaggtgacaacgtaaaaagccctctgaaaatcagagagctccagtcttgctggataaaccgtttatagactaggcgacgaggcgaagattgtacaaaatttttagtgaaacaaaaaaatctccccgtagggagaatatctggtttattttaccttacagtgctaggaaccgtaaccaaagataatacttgagcatatcaaggtaaggtgacaacgtaaaaagccctctgaaaatcagagagctacagtcttgctggataaaccgtttatagactaggcgacgaggcgaagattgtacaaaatttttagtgaaacaaaaaaatctccccgaagggagatcattctggtttattttaccttacagtgctaggaaccgtaaccgaagataatacttgagtatatcgaggtaaggtgacaacgcaatgtaagtggaaaataaagcaga Predict ORF larger than 30AA ================================================ Protein_Len: 36 Strand: + Start: 59 End: 166 .......................................................... M N T H L S S D D L N F F K T K N L P V G R I S G L F Y L T V L G T V T ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 31 Strand: + Start: 282 End: 374 ......................................................................................................................................................................................................................................................................................... M Y K I F S E T K K S P R R E N I W F I L P Y S A R N R N Q R ....................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: - Start: 350 End: 544 ............................................................................................................................................................................................................................................................................................................................................................. L W L Y Y K L M D L Y P S L T F L G R F I L S S C D Q Q I F R K Y V L R R P S S Q V F N K T F C F F R G S P L D N Q N M ............................................................................................. Protein_Len: 60 Strand: - Start: 147 End: 329 .................................................................................................................................................. A L F R L R L N Y K L M G L Y P S L T F L G R F I L S S W D Q Q I F R K Y V L R R P S S Q V F N K T F C F F R G T P L M .................................................................................................................................................................................................................................................................................................................... ttacttcctcctaggtgagcgagcacttacttttttaaagataaatctattatagtgagtgaatactcacttgtcaagtgatgatttgaatttttttaaaacaaaaaatctccccgtagggagaatatctggtttattttaccttacagtgctaggaaccgtaacctaagattatacttgagcataccaaggtaaggtgacaacgtaaaaagccctctgaaaatcagagagctccagtcttgctggataaaccgtttatagactaggcgacgaggcgaagattgtacaaaatttttagtgaaacaaaaaaatctccccgtagggagaatatctggtttattttaccttacagtgctaggaaccgtaaccaaagataatacttgagcatatcaaggtaaggtgacaacgtaaaaagccctctgaaaatcagagagctacagtcttgctggataaaccgtttatagactaggcgacgaggcgaagattgtacaaaatttttagtgaaacaaaaaaatctccccgaagggagatcattctggtttattttaccttacagtgctaggaaccgtaaccgaagataatacttgagtatatcgaggtaaggtgacaacgcaatgtaagtggaaaataaagcaga Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: - Score: 90 Start: 49 End: 78 ................................................CTTGACAAGTGAGTATTCACTCACTATAAT............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ttacttcctcctaggtgagcgagcacttacttttttaaagataaatctattatagtgagtgaatactcacttgtcaagtgatgatttgaatttttttaaaacaaaaaatctccccgtagggagaatatctggtttattttaccttacagtgctaggaaccgtaacctaagattatacttgagcataccaaggtaaggtgacaacgtaaaaagccctctgaaaatcagagagctccagtcttgctggataaaccgtttatagactaggcgacgaggcgaagattgtacaaaatttttagtgaaacaaaaaaatctccccgtagggagaatatctggtttattttaccttacagtgctaggaaccgtaaccaaagataatacttgagcatatcaaggtaaggtgacaacgtaaaaagccctctgaaaatcagagagctacagtcttgctggataaaccgtttatagactaggcgacgaggcgaagattgtacaaaatttttagtgaaacaaaaaaatctccccgaagggagatcattctggtttattttaccttacagtgctaggaaccgtaaccgaagataatacttgagtatatcgaggtaaggtgacaacgcaatgtaagtggaaaataaagcaga Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 100 HP_score: -9.0 Tail_Score: -5.79595 Start: 109 End: 124 Full_Region: aaataaaccagatat tctccc tacg gggaga ttttttgttttaaaa ............................................................................................................tctccctacggggaga................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. TransTerm Strand: - Conf: 100 HP_score: -11.9 Tail_Score: -4.78149 Start: 212 End: 232 Full_Region: tccagcaagactgga gctctctga ttt tcagagggc tttttacgttgtcac ...................................................................................................................................................................................................................gctctctgattttcagagggc..................................................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: - Conf: 100 HP_score: -9.0 Tail_Score: -5.96894 Start: 312 End: 327 Full_Region: aaataaaccagatat tctccc tacg gggaga tttttttgtttcact .......................................................................................................................................................................................................................................................................................................................tctccctacggggaga...................................................................................................................................................................................................................................................................................................................... TransTerm Strand: - Conf: 100 HP_score: -11.9 Tail_Score: -4.78149 Start: 415 End: 435 Full_Region: tccagcaagactgta gctctctga ttt tcagagggc tttttacgttgtcac ..............................................................................................................................................................................................................................................................................................................................................................................................................................gctctctgattttcagagggc.......................................................................................................................................................................................................... TransTerm Strand: - Conf: 100 HP_score: -9.0 Tail_Score: -5.96894 Start: 515 End: 530 Full_Region: aataaaccagaatga tctccc ttcg gggaga tttttttgtttcact ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tctcccttcggggaga........................................................................................................... Find igs database================================================ Query_seq: SMT_968:SMT_969|SMT_968:SMT_969:K01990 ABC-2 type transport system ATP-binding protein:30S ribosomal protein S15:<-<-:914106..914742 637 ttacttcctcctaggtgagcgagcacttacttttttaaagataaatctattatagtgagtgaatactcacttgtcaagtgatgatttgaatttttttaaaacaaaaaatctccccgtagggagaatatctggtttattttaccttacagtgctaggaaccgtaacctaagattatacttgagcataccaaggtaaggtgacaacgtaaaaagccctctgaaaatcagagagctccagtcttgctggataaaccgtttatagactaggcgacgaggcgaagattgtacaaaatttttagtgaaacaaaaaaatctccccgtagggagaatatctggtttattttaccttacagtgctaggaaccgtaaccaaagataatacttgagcatatcaaggtaaggtgacaacgtaaaaagccctctgaaaatcagagagctacagtcttgctggataaaccgtttatagactaggcgacgaggcgaagattgtacaaaatttttagtgaaacaaaaaaatctccccgaagggagatcattctggtttattttaccttacagtgctaggaaccgtaaccgaagataatacttgagtatatcgaggtaaggtgacaacgcaatgtaagtggaaaataaagcaga Intra-Species Hit: Count: 2 Min: 1 Max: 637 Len: 637 Subject: smit_SMT_968_SMT_969|K01990 ABC-2 type transport system ATP-binding protein:30S ribosomal protein S15|NEGATIVE:NEGATIVE|[914106,914742]|637 HSP 1 e-value: 0.0 bit: 1138.0 Len: 637 Query Start:1 Query End:637 Subject Strand: POSITIVE Subject Start: 1 Subject End: 637 ttacttcctcctaggtgagcgagcacttacttttttaaagataaatctattatagtgagtgaatactcacttgtcaagtgatgatttgaannnnnnnaaaacaaaaaatctccccgtagggagaatatctggtttattttaccttacagtgctaggaaccgtaacctaagattatacttgagcataccaaggtaaggtgacaacgtaaaaagccctctgaaaatcagagagctccagtcttgctggataaaccgtttatagactaggcgacgaggcgaagattgtacaaaatttttagtgaaacnnnnnnntctccccgtagggagaatatctggtttattttaccttacagtgctaggaaccgtaaccaaagataatacttgagcatatcaaggtaaggtgacaacgtaaaaagccctctgaaaatcagagagctacagtcttgctggataaaccgtttatagactaggcgacgaggcgaagattgtacaaaatttttagtgaaacnnnnnnntctccccgaagggagatcattctggtttattttaccttacagtgctaggaaccgtaaccgaagataatacttgagtatatcgaggtaaggtgacaacgcaatgtaagtggaaaataaagcaga ttacttcctcctaggtgagcgagcacttacttttttaaagataaatctattatagtgagtgaatactcacttgtcaagtgatgatttgaatttttttaaaacaaaaaatctccccgtagggagaatatctggtttattttaccttacagtgctaggaaccgtaacctaagattatacttgagcataccaaggtaaggtgacaacgtaaaaagccctctgaaaatcagagagctccagtcttgctggataaaccgtttatagactaggcgacgaggcgaagattgtacaaaatttttagtgaaacaaaaaaatctccccgtagggagaatatctggtttattttaccttacagtgctaggaaccgtaaccaaagataatacttgagcatatcaaggtaaggtgacaacgtaaaaagccctctgaaaatcagagagctacagtcttgctggataaaccgtttatagactaggcgacgaggcgaagattgtacaaaatttttagtgaaacaaaaaaatctccccgaagggagatcattctggtttattttaccttacagtgctaggaaccgtaaccgaagataatacttgagtatatcgaggtaaggtgacaacgcaatgtaagtggaaaataaagcaga Subject: smit_SMT_963_SMT_964|K02015 iron complex transport system permease protein:cadmium resistance transporter, putative|NEGATIVE:NEGATIVE|[909723,909980]|258 HSP 1 e-value: 9.0E-36 bit: 153.0 Len: 89 Query Start:348 Query End:436 Subject Strand: POSITIVE Subject Start: 130 Subject End: 218 ...........................................................................................................................................................................................................................................................................................................................................................tacagtgctaggaaccgtaaccaaagataatacttgagcatatcaaggtaaggtgacaacgtaaaaagccctctgaaaatcagagagct......................................................................................................................................................................................................... ...........................................................................................................................................................................................................................................................................................................................................................tacagttctaggaaccgtaaccaaagataatacttgagcataccaaggtaaggtgacaatgtaaaaagccctctgaaaatcagagagct......................................................................................................................................................................................................... HSP 2 e-value: 2.0E-33 bit: 145.0 Len: 89 Query Start:145 Query End:233 Subject Strand: POSITIVE Subject Start: 130 Subject End: 218 ................................................................................................................................................tacagtgctaggaaccgtaacctaagattatacttgagcataccaaggtaaggtgacaacgtaaaaagccctctgaaaatcagagagct.................................................................................................................................................................................................................................................................................................................................................................................................................... ................................................................................................................................................tacagttctaggaaccgtaaccaaagataatacttgagcataccaaggtaaggtgacaatgtaaaaagccctctgaaaatcagagagct.................................................................................................................................................................................................................................................................................................................................................................................................................... HSP 3 e-value: 4.0E-13 bit: 77.8 Len: 59 Query Start:552 Query End:610 Subject Strand: POSITIVE Subject Start: 130 Subject End: 188 .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tacagtgctaggaaccgtaaccgaagataatacttgagtatatcgaggtaaggtgacaa........................... .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tacagttctaggaaccgtaaccaaagataatacttgagcataccaaggtaaggtgacaa........................... Inter-species Hit: Count: 7 Min: 98 Max: 615 Len: 518 Subject: NC_003028_SP_1625_SP_1626|cadmium resistance transporter, putative:30S ribosomal protein S15|NEGATIVE:NEGATIVE|[1527453,1528267]|815 HSP 1 e-value: 1.0E-47 bit: 192.0 Len: 177 Query Start:238 Query End:414 Subject Strand: POSITIVE Subject Start: 483 Subject End: 660 .............................................................................................................................................................................................................................................tcttgctggataaaccgtttatagactaggcgacgaggcgaagattgtacaaaatttttagtgaaacnnnnnnn-tctccccgtagggagaatatctggtttattttaccttacagtgctaggaaccgtaaccaaagataatacttgagcatatcaaggtaaggtgacaacgtaaaaa............................................................................................................................................................................................................................... .............................................................................................................................................................................................................................................tcttgctagataaatcgtttatagactaggcgacgaggcgaagatcgtacgaaactttcagtgaaacaaaaaaaatctccccgaagggagaaaagctggtttattttaccttacagtgctaggaaccgtaaccaaagattatacttgagtatatcgaggtaaggtgacaacgcaaaaa............................................................................................................................................................................................................................... HSP 2 e-value: 2.0E-46 bit: 188.0 Len: 175 Query Start:441 Query End:615 Subject Strand: POSITIVE Subject Start: 483 Subject End: 657 ........................................................................................................................................................................................................................................................................................................................................................................................................................................................tcttgctggataaaccgtttatagactaggcgacgaggcgaagattgtacaaaatttttagtgaaacnnnnnnn-tctccccgaagggagatcattctggtttattttaccttacagtgctaggaaccgtaaccgaagataatacttgagtatatcgaggtaaggtgacaacgcaa...................... ........................................................................................................................................................................................................................................................................................................................................................................................................................................................tcttgctagataaatcgtttatagactaggcgacgaggcgaagatcgtacgaaactttcagtgaaacaaaaaaaatctccccgaagggagaaaag-ctggtttattttaccttacagtgctaggaaccgtaaccaaagattatacttgagtatatcgaggtaaggtgacaacgcaa...................... HSP 3 e-value: 9.0E-36 bit: 153.0 Len: 109 Query Start:103 Query End:211 Subject Strand: POSITIVE Subject Start: 552 Subject End: 660 ......................................................................................................aaaaaatctccccgtagggagaatatctggtttattttaccttacagtgctaggaaccgtaacctaagattatacttgagcataccaaggtaaggtgacaacgtaaaaa.......................................................................................................................................................................................................................................................................................................................................................................................................................................... ......................................................................................................aaaaaatctccccgaagggagaaaagctggtttattttaccttacagtgctaggaaccgtaaccaaagattatacttgagtatatcgaggtaaggtgacaacgcaaaaa.......................................................................................................................................................................................................................................................................................................................................................................................................................................... Subject: NC_008533_SPD_1438_SPD_1439|cadmium resistance transporter, putative:ribosomal protein S15|NEGATIVE:NEGATIVE|[1456387,1457119]|733 HSP 1 e-value: 3.0E-45 bit: 184.0 Len: 177 Query Start:238 Query End:414 Subject Strand: POSITIVE Subject Start: 482 Subject End: 659 .............................................................................................................................................................................................................................................tcttgctggataaaccgtttatagactaggcgacgaggcgaagattgtacaaaatttttagtgaaacnnnnnnn-tctccccgtagggagaatatctggtttattttaccttacagtgctaggaaccgtaaccaaagataatacttgagcatatcaaggtaaggtgacaacgtaaaaa............................................................................................................................................................................................................................... .............................................................................................................................................................................................................................................tcttgctagataaatcgtttatagactaggcgacgaggcgaagatcgtacgaaactttcagtgaaacaaaaaaaatctccccgaagggagaaaagctggtttattttaccttacagtgctaggaaccgtaaccaaagattatacttgagtatagcgaggtaaggtgacaacgcaaaaa............................................................................................................................................................................................................................... HSP 2 e-value: 4.0E-44 bit: 180.0 Len: 175 Query Start:441 Query End:615 Subject Strand: POSITIVE Subject Start: 482 Subject End: 656 ........................................................................................................................................................................................................................................................................................................................................................................................................................................................tcttgctggataaaccgtttatagactaggcgacgaggcgaagattgtacaaaatttttagtgaaacnnnnnnn-tctccccgaagggagatcattctggtttattttaccttacagtgctaggaaccgtaaccgaagataatacttgagtatatcgaggtaaggtgacaacgcaa...................... ........................................................................................................................................................................................................................................................................................................................................................................................................................................................tcttgctagataaatcgtttatagactaggcgacgaggcgaagatcgtacgaaactttcagtgaaacaaaaaaaatctccccgaagggagaaaag-ctggtttattttaccttacagtgctaggaaccgtaaccaaagattatacttgagtatagcgaggtaaggtgacaacgcaa...................... HSP 3 e-value: 9.0E-36 bit: 153.0 Len: 109 Query Start:103 Query End:211 Subject Strand: POSITIVE Subject Start: 551 Subject End: 659 ......................................................................................................aaaaaatctccccgtagggagaatatctggtttattttaccttacagtgctaggaaccgtaacctaagattatacttgagcataccaaggtaaggtgacaacgtaaaaa.......................................................................................................................................................................................................................................................................................................................................................................................................................................... ......................................................................................................aaaaaatctccccgaagggagaaaagctggtttattttaccttacagtgctaggaaccgtaaccaaagattatacttgagtatagcgaggtaaggtgacaacgcaaaaa.......................................................................................................................................................................................................................................................................................................................................................................................................................................... Subject: NC_003098_spr1466_spr1467|Confers low level cadmium resistance in Staphylococcus aureus:30S ribosomal protein S15|NEGATIVE:NEGATIVE|[1448856,1449588]|733 HSP 1 e-value: 3.0E-45 bit: 184.0 Len: 177 Query Start:238 Query End:414 Subject Strand: POSITIVE Subject Start: 482 Subject End: 659 .............................................................................................................................................................................................................................................tcttgctggataaaccgtttatagactaggcgacgaggcgaagattgtacaaaatttttagtgaaacnnnnnnn-tctccccgtagggagaatatctggtttattttaccttacagtgctaggaaccgtaaccaaagataatacttgagcatatcaaggtaaggtgacaacgtaaaaa............................................................................................................................................................................................................................... .............................................................................................................................................................................................................................................tcttgctagataaatcgtttatagactaggcgacgaggcgaagatcgtacgaaactttcagtgaaacaaaaaaaatctccccgaagggagaaaagctggtttattttaccttacagtgctaggaaccgtaaccaaagattatacttgagtatagcgaggtaaggtgacaacgcaaaaa............................................................................................................................................................................................................................... HSP 2 e-value: 4.0E-44 bit: 180.0 Len: 175 Query Start:441 Query End:615 Subject Strand: POSITIVE Subject Start: 482 Subject End: 656 ........................................................................................................................................................................................................................................................................................................................................................................................................................................................tcttgctggataaaccgtttatagactaggcgacgaggcgaagattgtacaaaatttttagtgaaacnnnnnnn-tctccccgaagggagatcattctggtttattttaccttacagtgctaggaaccgtaaccgaagataatacttgagtatatcgaggtaaggtgacaacgcaa...................... ........................................................................................................................................................................................................................................................................................................................................................................................................................................................tcttgctagataaatcgtttatagactaggcgacgaggcgaagatcgtacgaaactttcagtgaaacaaaaaaaatctccccgaagggagaaaag-ctggtttattttaccttacagtgctaggaaccgtaaccaaagattatacttgagtatagcgaggtaaggtgacaacgcaa...................... HSP 3 e-value: 9.0E-36 bit: 153.0 Len: 109 Query Start:103 Query End:211 Subject Strand: POSITIVE Subject Start: 551 Subject End: 659 ......................................................................................................aaaaaatctccccgtagggagaatatctggtttattttaccttacagtgctaggaaccgtaacctaagattatacttgagcataccaaggtaaggtgacaacgtaaaaa.......................................................................................................................................................................................................................................................................................................................................................................................................................................... ......................................................................................................aaaaaatctccccgaagggagaaaagctggtttattttaccttacagtgctaggaaccgtaaccaaagattatacttgagtatagcgaggtaaggtgacaacgcaaaaa.......................................................................................................................................................................................................................................................................................................................................................................................................................................... Subject: NC_009009_SSA_2056_SSA_2058|Cinnamoyl ester hydrolase, putative:30S ribosomal protein S15, putative|POSITIVE:NEGATIVE|[2059654,2060147]|494 HSP 1 e-value: 6.0E-34 bit: 147.0 Len: 94 Query Start:515 Query End:608 Subject Strand: POSITIVE Subject Start: 257 Subject End: 349 ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tctccccgaagggagatcattctggtttattttaccttacagtgctaggaaccgtaaccgaagataatacttgagtatatcgaggtaaggtgac............................. ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tctcccctaagggagaagatt-tggtttattttaccttacagtgctaggaaccgtaaccgaagataatacttgagtatatcgaggtaaggtgac............................. HSP 2 e-value: 1.0E-28 bit: 129.0 Len: 93 Query Start:312 Query End:404 Subject Strand: POSITIVE Subject Start: 257 Subject End: 349 .......................................................................................................................................................................................................................................................................................................................tctccccgtagggagaatatctggtttattttaccttacagtgctaggaaccgtaaccaaagataatacttgagcatatcaaggtaaggtgac......................................................................................................................................................................................................................................... .......................................................................................................................................................................................................................................................................................................................tctcccctaagggagaagatttggtttattttaccttacagtgctaggaaccgtaaccgaagataatacttgagtatatcgaggtaaggtgac......................................................................................................................................................................................................................................... HSP 3 e-value: 5.0E-28 bit: 127.0 Len: 104 Query Start:98 Query End:201 Subject Strand: POSITIVE Subject Start: 246 Subject End: 349 .................................................................................................aaaacaaaaaatctccccgtagggagaatatctggtttattttaccttacagtgctaggaaccgtaacctaagattatacttgagcataccaaggtaaggtgac.................................................................................................................................................................................................................................................................................................................................................................................................................................................... .................................................................................................aaaataaaaaatctcccctaagggagaagatttggtttattttaccttacagtgctaggaaccgtaaccgaagataatacttgagtatatcgaggtaaggtgac.................................................................................................................................................................................................................................................................................................................................................................................................................................................... HSP 4 e-value: 2.0E-9 bit: 65.9 Len: 81 Query Start:207 Query End:287 Subject Strand: POSITIVE Subject Start: 151 Subject End: 231 ..............................................................................................................................................................................................................aaaaagccctctgaaaatcagagagctccagtcttgctggataaaccgtttatagactaggcgacgaggcgaagattgtac.............................................................................................................................................................................................................................................................................................................................................................. ..............................................................................................................................................................................................................aaaaagcactctacaaataagagtgctccattcttgcaggataaaatgtttctagacaaggcgacgagccgaagattgtac.............................................................................................................................................................................................................................................................................................................................................................. HSP 5 e-value: 4.0E-7 bit: 58.0 Len: 81 Query Start:410 Query End:490 Subject Strand: POSITIVE Subject Start: 151 Subject End: 231 .........................................................................................................................................................................................................................................................................................................................................................................................................................aaaaagccctctgaaaatcagagagctacagtcttgctggataaaccgtttatagactaggcgacgaggcgaagattgtac................................................................................................................................................... .........................................................................................................................................................................................................................................................................................................................................................................................................................aaaaagcactctacaaataagagtgctccattcttgcaggataaaatgtttctagacaaggcgacgagccgaagattgtac................................................................................................................................................... Subject: NC_008533_SPD_1437_SPD_1438|acyltransferase domain protein:cadmium resistance transporter, putative|POSITIVE:NEGATIVE|[1455480,1455771]|292 HSP 1 e-value: 6.0E-6 bit: 54.0 Len: 27 Query Start:207 Query End:233 Subject Strand: POSITIVE Subject Start: 226 Subject End: 252 ..............................................................................................................................................................................................................aaaaagccctctgaaaatcagagagct.................................................................................................................................................................................................................................................................................................................................................................................................................... ..............................................................................................................................................................................................................aaaaagccctctgaaaatcagagagct.................................................................................................................................................................................................................................................................................................................................................................................................................... HSP 2 e-value: 6.0E-6 bit: 54.0 Len: 27 Query Start:410 Query End:436 Subject Strand: POSITIVE Subject Start: 226 Subject End: 252 .........................................................................................................................................................................................................................................................................................................................................................................................................................aaaaagccctctgaaaatcagagagct......................................................................................................................................................................................................... .........................................................................................................................................................................................................................................................................................................................................................................................................................aaaaagccctctgaaaatcagagagct......................................................................................................................................................................................................... Subject: NC_003098_spr1465_spr1466|hypothetical protein:Confers low level cadmium resistance in Staphylococcus aureus|POSITIVE:NEGATIVE|[1447949,1448240]|292 HSP 1 e-value: 6.0E-6 bit: 54.0 Len: 27 Query Start:207 Query End:233 Subject Strand: POSITIVE Subject Start: 226 Subject End: 252 ..............................................................................................................................................................................................................aaaaagccctctgaaaatcagagagct.................................................................................................................................................................................................................................................................................................................................................................................................................... ..............................................................................................................................................................................................................aaaaagccctctgaaaatcagagagct.................................................................................................................................................................................................................................................................................................................................................................................................................... HSP 2 e-value: 6.0E-6 bit: 54.0 Len: 27 Query Start:410 Query End:436 Subject Strand: POSITIVE Subject Start: 226 Subject End: 252 .........................................................................................................................................................................................................................................................................................................................................................................................................................aaaaagccctctgaaaatcagagagct......................................................................................................................................................................................................... .........................................................................................................................................................................................................................................................................................................................................................................................................................aaaaagccctctgaaaatcagagagct......................................................................................................................................................................................................... Subject: NC_003028_SP_1624_SP_1625|acyltransferase family protein:cadmium resistance transporter, putative|POSITIVE:NEGATIVE|[1526547,1526837]|291 HSP 1 e-value: 6.0E-6 bit: 54.0 Len: 27 Query Start:207 Query End:233 Subject Strand: POSITIVE Subject Start: 225 Subject End: 251 ..............................................................................................................................................................................................................aaaaagccctctgaaaatcagagagct.................................................................................................................................................................................................................................................................................................................................................................................................................... ..............................................................................................................................................................................................................aaaaagccctctgaaaatcagagagct.................................................................................................................................................................................................................................................................................................................................................................................................................... HSP 2 e-value: 6.0E-6 bit: 54.0 Len: 27 Query Start:410 Query End:436 Subject Strand: POSITIVE Subject Start: 225 Subject End: 251 .........................................................................................................................................................................................................................................................................................................................................................................................................................aaaaagccctctgaaaatcagagagct......................................................................................................................................................................................................... .........................................................................................................................................................................................................................................................................................................................................................................................................................aaaaagccctctgaaaatcagagagct......................................................................................................................................................................................................... Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAAACAAAAAAUCUCCCCGUAGGGAGAAUAUCUGGUUUAUUUUACCUUACAGUGCUAGGAACCGUAACCUAAGAUUAUACUUGAGCAUACCAAGGUAAGGUGACAACGUAAAAAGCCCUCUGAAAAUCAGAGAGCUCCAGUCUUGCUGGAUAAACCGUUUAUAGACUAGGCGACGAGGCGAAGAUUGUACAAAAUUUUUAGUGAAACAAAAAAAUCUCCCCGUAGGGAGAAUAUCUGGUUUAUUUUACCUUACAGUGCUAGGAACCGUAACCAAAGAUAAUACUUGAGCAUAUCAAGGUAAGGUGACAACGUAAAAAGCCCUCUGAAAAUCAGAGAGCUACAGUCUUGCUGGAUAAACCGUUUAUAGACUAGGCGACGAGGCGAAGAUUGUACAAAAUUUUUAGUGAAACAAAAAAAUCUCCCCGAAGGGAGAUCAUUCUGGUUUAUUUUACCUUACAGUGCUAGGAACCGUAACCGAAGAUAAUACUUGAGUAUAUCGAGGUAAGGUGACAACGCAA .((((......((((((....)))))).......))))...(((((((((.....((((........)))).(((.(((((..((.....((..(((((((((.((((.....(((.(((((.....))))).)))((((.....))))......)))).((((((((((...((..(.(((..((((((((..(((.(..((...........((((((....))))))...((((((((((.(((((((((.......((........))..........(((((.....)))))))))))))).((((.....(((.(((((.....))))).))).(((.....))).......)))).))))))))))...))..).)))..))))))))..))).)..))..........(((((((....)))))))...)))))))))).)))))))))..))...((........))..........))..))))))))...)))))))))........ (-142.42) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUGCGUUGUCACCUUACCUCGAUAUACUCAAGUAUUAUCUUCGGUUACGGUUCCUAGCACUGUAAGGUAAAAUAAACCAGAAUGAUCUCCCUUCGGGGAGAUUUUUUUGUUUCACUAAAAAUUUUGUACAAUCUUCGCCUCGUCGCCUAGUCUAUAAACGGUUUAUCCAGCAAGACUGUAGCUCUCUGAUUUUCAGAGGGCUUUUUACGUUGUCACCUUACCUUGAUAUGCUCAAGUAUUAUCUUUGGUUACGGUUCCUAGCACUGUAAGGUAAAAUAAACCAGAUAUUCUCCCUACGGGGAGAUUUUUUUGUUUCACUAAAAAUUUUGUACAAUCUUCGCCUCGUCGCCUAGUCUAUAAACGGUUUAUCCAGCAAGACUGGAGCUCUCUGAUUUUCAGAGGGCUUUUUACGUUGUCACCUUACCUUGGUAUGCUCAAGUAUAAUCUUAGGUUACGGUUCCUAGCACUGUAAGGUAAAAUAAACCAGAUAUUCUCCCUACGGGGAGAUUUUUUGUUUU ..........(((((((...(((((((....))).))))....((((.((..((((((...(((((((...(((((((.....((((((((....))))))))..(((((...((((......((((((((.....((......)).((((..(((((.(((((((.((((........(((((((((.....)))))))))......))))..(((((((...(((((((....))).))))....((((.(....)))))...)))))))...)))))))......((((((....))))))....)))))...))))......)))))))).....((......)).))))..))))).))))))).((((......(((((((((((.....)))))))))))....))))..))))))).....(((((....))))).......)))).))..))))))...))))))).........((((...((((((....))))))...)))).... (-151.74) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 659 43 1 231 189 smit:SMT_968|5end_K01990_ABC-2_type_transport_system_ATP-binding_protein_914015..914245_NEGATIVE 350 510 461 183 136 smit:SMT_1782|5end_hypothetical_protein_1759520..1759750_POSITIVE 346 148 102 226 180 smit:SMT_1605|5end_hypothetical_protein_1595406..1595636_POSITIVE 343 185 141 73 27 smit:SMT_868|5end_D-alanyl-D-alanine_carboxypeptidase_817225..817455_NEGATIVE 339 158 115 70 28 smit:SMT_145|5end_hypothetical_protein_95873..96103_POSITIVE 324 470 423 137 88 smit:SMT_1920|5end_ABC_transporter,_ATP-binding_protein_1863125..1863355_NEGATIVE 321 161 130 119 86 smit:SMT_155|5end_hypothetical_protein_102704..102934_NEGATIVE 310 173 134 102 62 smit:SMT_1673|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1659831..1660061_POSITIVE 309 179 134 165 130 smit:SMT_890|5end_K02029_polar_amino_acid_transport_system_permease_protein_836953..837183_POSITIVE 307 180 131 213 164 smit:SMT_416|5end_ribonuclease_Z_359423..359653_POSITIVE 306 179 135 45 5 smit:SMT_2082|5end_ATP-dependent_nuclease_subunit_B_1982286..1982516_NEGATIVE 305 179 133 89 49 smit:SMT_929|5end_hypothetical_protein_883342..883572_NEGATIVE 305 179 133 213 173 smit:SMT_928|5end_hypothetical_protein_883218..883448_NEGATIVE 303 510 461 185 150 smit:SMT_1963|5end_ribonuclease_BN,_putative_1896462..1896692_NEGATIVE 303 510 461 172 137 smit:SMT_483|5end_transcriptional_activator_TipA,_putative_418827..419057_POSITIVE 301 188 145 86 36 smit:SMT_1614|5end_K08998_hypothetical_protein_1601330..1601560_POSITIVE 299 510 461 220 185 smit:SMT_1810|5end_hypothetical_protein_1785219..1785449_POSITIVE 299 110 62 208 168 smit:SMT_1488|5end_K06147_ATP-binding_cassette,_subfamily_B,_bacterial_1467157..1467387_POSITIVE 298 150 105 172 124 smit:SMT_271|5end_autoinducer-2_production_protein_211736..211966_NEGATIVE 296 380 336 61 11 smit:SMT_2124|5end_hypothetical_protein_2008442..2008672_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 790 339 291 50 2 smit:SMT_964|3end_cadmium_resistance_transporter,_putative_909891..910041_NEGATIVE 536 190 144 47 1 smit:SMT_965|3end_putative_transposase_911085..911235_NEGATIVE 343 185 141 70 24 smit:SMT_869|3end_hypothetical_protein_817228..817378_NEGATIVE 330 100 51 53 8 smit:SMT_915|3end_sucrose_operon_repressor_868619..868769_POSITIVE 319 179 131 67 6 smit:SMT_1302|3end_hypothetical_protein_1264649..1264799_POSITIVE 310 109 62 41 2 smit:SMT_1671|3end_hypothetical_protein_1659392..1659542_NEGATIVE 305 179 133 42 2 smit:SMT_930|3end_ATP-dependent_RNA_helicase_883389..883539_NEGATIVE 303 510 461 51 16 smit:SMT_1964|3end_sodium/hydrogen_exchanger_family_protein_1896596..1896746_NEGATIVE 303 510 461 53 18 smit:SMT_799|3end_hypothetical_protein_707296..707446_NEGATIVE 301 188 145 86 36 smit:SMT_1613|3end_ribosomal_large_subunit_pseudouridine_synthase_B_1601410..1601560_POSITIVE 299 510 461 55 20 smit:SMT_1809|3end_MutT/NUDIX_family_protein_1785134..1785284_POSITIVE 299 110 62 47 7 smit:SMT_1487|3end_hypothetical_protein_1467076..1467226_POSITIVE 298 150 105 77 29 smit:SMT_272|3end_hypothetical_protein_211831..211981_NEGATIVE 296 380 336 80 30 smit:SMT_2123|3end_hypothetical_protein_2008541..2008691_POSITIVE 296 395 351 61 15 smit:SMT_772|3end_spermidine/putrescine_ABC_transporter,_ATP-binding_protein_682143..682293_NEGATIVE 295 510 461 56 9 smit:SMT_834|3end_hypothetical_protein_775517..775667_NEGATIVE 293 510 461 51 16 smit:SMT_1536|3end_trigger_factor_1520636..1520786_NEGATIVE 292 380 337 50 4 smit:SMT_752|3end_prephenate_dehydrogenase_655688..655838_NEGATIVE 292 109 62 64 25 smit:SMT_698|3end_uracil_permease_609769..609919_POSITIVE 285 178 134 150 120 smit:SMT_889|3end_K02029_polar_amino_acid_transport_system_permease_protein_837023..837173_POSITIVE