Origin IGS: acaaaggaaaatgatatcaaagaactccgttatcttgtcggagttctttttgtctgttcttttacccttctcccttgcattttctctcattttttgccaaaatagaggggtagaaagaaggtagcat .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 atgctaccttctttctacccctctattttggcaaaaaatgagagaaaatgcaagggagaagggtaaaagaacagacaaaaagaactccgacaagataacggagttctttgatatcattttcctttgt Mask Tandem Repeat Region ================================================ acaaaggaaaatgatatcaaagaactccgttatcttgtcggagttctttttgtctgttcttttacccttctcccttgcattttctctcattttttgccaaaatagaggggtagaaagaaggtagcat Find is-nt database================================================ Query_seq: SMT_936:SMT_937|SMT_936:SMT_937:cobyric acid synthase:dipeptidase:->->:889592..889718 127 acaaaggaaaatgatatcaaagaactccgttatcttgtcggagttctttttgtctgttcttttacccttctcccttgcattttctctcattttttgccaaaatagaggggtagaaagaaggtagcat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_936:SMT_937|SMT_936:SMT_937:cobyric acid synthase:dipeptidase:->->:889592..889718 127 acaaaggaaaatgatatcaaagaactccgttatcttgtcggagttctttttgtctgttcttttacccttctcccttgcattttctctcattttttgccaaaatagaggggtagaaagaaggtagcat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_936:SMT_937|SMT_936:SMT_937:cobyric acid synthase:dipeptidase:->->:889592..889718 127 acaaaggaaaatgatatcaaagaactccgttatcttgtcggagttctttttgtctgttcttttacccttctcccttgcattttctctcattttttgccaaaatagaggggtagaaagaaggtagcat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 acaaaggaaaatgatatcaaagaactccgttatcttgtcggagttctttttgtctgttcttttacccttctcccttgcattttctctcattttttgccaaaatagaggggtagaaagaaggtagcat Predict ORF larger than 30AA ================================================ Protein_Len: 37 Strand: + Start: 11 End: 121 .......... M I S K N S V I L S E F F L S V L L P F S L A F S L I F C Q N R G V E R R ...... Protein_Len: 30 Strand: - Start: 1 End: 90 C L F I I D F F E T I K D S N K K D T R K G K E R A N E R M ..................................... acaaaggaaaatgatatcaaagaactccgttatcttgtcggagttctttttgtctgttcttttacccttctcccttgcattttctctcattttttgccaaaatagaggggtagaaagaaggtagcat Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ acaaaggaaaatgatatcaaagaactccgttatcttgtcggagttctttttgtctgttcttttacccttctcccttgcattttctctcattttttgccaaaatagaggggtagaaagaaggtagcat Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 71 HP_score: -7.2 Tail_Score: -3.92694 Start: 22 End: 46 Full_Region: aagaacagacaaaaa gaactccg acaagataa cggagttc tttgatatcattttc .....................gaactccgacaagataacggagttc................................................................................. Find igs database================================================ Query_seq: SMT_936:SMT_937|SMT_936:SMT_937:cobyric acid synthase:dipeptidase:->->:889592..889718 127 acaaaggaaaatgatatcaaagaactccgttatcttgtcggagttctttttgtctgttcttttacccttctcccttgcattttctctcattttttgccaaaatagaggggtagaaagaaggtagcat Intra-Species Hit: Count: 2 Min: 1 Max: 127 Len: 127 Subject: smit_SMT_936_SMT_937|cobyric acid synthase:dipeptidase|POSITIVE:POSITIVE|[889592, 889718]|127 HSP 1 e-value: 2.0E-66 bit: 252.0 Len: 127 Query Start:1 Query End:127 Subject Strand: POSITIVE Subject Start: 1 Subject End: 127 acaaaggaaaatgatatcaaagaactccgttatcttgtcggagttctttttgtctgttcttttacccttctcccttgcattttctctcattttttgccaaaatagaggggtagaaagaaggtagcat acaaaggaaaatgatatcaaagaactccgttatcttgtcggagttctttttgtctgttcttttacccttctcccttgcattttctctcattttttgccaaaatagaggggtagaaagaaggtagcat Subject: smit_SMT_931_SMT_932|cobyric acid synthase:tellurite resistance protein|POSITIVE:POSITIVE|[885078,885204]|127 HSP 1 e-value: 2.0E-66 bit: 252.0 Len: 127 Query Start:1 Query End:127 Subject Strand: POSITIVE Subject Start: 1 Subject End: 127 acaaaggaaaatgatatcaaagaactccgttatcttgtcggagttctttttgtctgttcttttacccttctcccttgcattttctctcattttttgccaaaatagaggggtagaaagaaggtagcat acaaaggaaaatgatatcaaagaactccgttatcttgtcggagttctttttgtctgttcttttacccttctcccttgcattttctctcattttttgccaaaatagaggggtagaaagaaggtagcat Inter-species Hit: Count: 3 Min: 1 Max: 127 Len: 127 Subject: NC_008533_SPD_1417_SPD_1418|hypothetical protein:proline dipeptidase PepQ|POSITIVE:POSITIVE|[1438049,1438173]|125 HSP 1 e-value: 2.0E-57 bit: 222.0 Len: 127 Query Start:1 Query End:127 Subject Strand: POSITIVE Subject Start: 1 Subject End: 125 acaaaggaaaatgatatcaaagaactccgttatcttgtcggagttctttttgtctgttcttttacccttctcccttgcattttctctcattttttgccaaaatagaggggtagaaagaaggtagcat acaaaggaaaatgatatcaaagaactccgttatcttgtcggagtt--ttttgtcttttcttttacccttctcccttgcattttctctcattttttgccaaaatagaggggtagaaagaaggtagcat Subject: NC_003098_spr1444_spr1445|Cobyric acid synthase:Dipeptidase|POSITIVE:POSITIVE|[1430518, 1430642]|125 HSP 1 e-value: 2.0E-57 bit: 222.0 Len: 127 Query Start:1 Query End:127 Subject Strand: POSITIVE Subject Start: 1 Subject End: 125 acaaaggaaaatgatatcaaagaactccgttatcttgtcggagttctttttgtctgttcttttacccttctcccttgcattttctctcattttttgccaaaatagaggggtagaaagaaggtagcat acaaaggaaaatgatatcaaagaactccgttatcttgtcggagtt--ttttgtcttttcttttacccttctcccttgcattttctctcattttttgccaaaatagaggggtagaaagaaggtagcat Subject: NC_003028_SP_1590_SP_1591|hypothetical protein:proline dipeptidase|POSITIVE:POSITIVE|[1496499,1496623]|125 HSP 1 e-value: 2.0E-57 bit: 222.0 Len: 127 Query Start:1 Query End:127 Subject Strand: POSITIVE Subject Start: 1 Subject End: 125 acaaaggaaaatgatatcaaagaactccgttatcttgtcggagttctttttgtctgttcttttacccttctcccttgcattttctctcattttttgccaaaatagaggggtagaaagaaggtagcat acaaaggaaaatgatatcaaagaactccgttatcttgtcggagtt--ttttgtcttttcttttacccttctcccttgcattttctctcattttttgccaaaatagaggggtagaaagaaggtagcat Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ ACAAAGGAAAAUGAUAUCAAAGAACUCCGUUAUCUUGUCGGAGUUCUUUUUGUCUGUUCUUUUACCCUUCUCCCUUGCAUUUUCUCUCAUUUUUUGCCAAAAUAGAGGGGUAGAAAGAAGGUAGCAU ..(((((((...((((..(((((((((((.........))))))))))).))))..)))))))..((((((..((.....(..((((.(((((.....)))))))))..).))..))))))...... (-37.70) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUGCUACCUUCUUUCUACCCCUCUAUUUUGGCAAAAAAUGAGAGAAAAUGCAAGGGAGAAGGGUAAAAGAACAGACAAAAAGAACUCCGACAAGAUAACGGAGUUCUUUGAUAUCAUUUUCCUUUGU ........(((((((....((........))........)))))))...(((((((((((.((((...(......)..(((((((((((.........)))))))))))..)))).))))))))))) (-33.70) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 319 90 41 204 143 smit:SMT_895|5end_cysteinyl-tRNA_synthetase_843037..843267_NEGATIVE 292 49 3 200 156 smit:SMT_932|5end_tellurite_resistance_protein_885065..885295_POSITIVE 289 78 35 116 72 smit:SMT_393|5end_hypothetical_protein_predicted_by_Glimmer/Critica_323973..324203_POSITIVE 279 68 21 164 122 smit:SMT_1705|5end_UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-_diaminopimelate--D-alanyl-D-alanine_ligase_1688558..1688788_POSITIVE 279 79 31 71 22 smit:SMT_1259|5end_hypothetical_protein_1225139..1225369_POSITIVE 277 118 72 144 82 smit:SMT_2055|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1965785..1966015_POSITIVE 276 88 42 109 50 smit:SMT_2163|5end_cell_division_protein_FtsA_2037643..2037873_POSITIVE 276 88 42 109 50 smit:SMT_2160|5end_cell_division_protein_FtsA_2033524..2033754_POSITIVE 276 88 42 109 50 smit:SMT_2158|5end_cell_division_protein_2031534..2031764_POSITIVE 276 88 42 109 50 smit:SMT_1707|5end_cell_division_protein_FtsA_1690945..1691175_POSITIVE 276 88 42 109 50 smit:SMT_1547|5end_cell_division_protein_FtsA_1530987..1531217_POSITIVE 276 80 32 55 8 smit:SMT_498|5end_hypothetical_protein_431751..431981_POSITIVE 270 106 61 184 136 smit:SMT_1619|5end_K02006_cobalt_transport_system_ATP-binding_protein_1606526..1606756_NEGATIVE 268 70 26 102 57 smit:SMT_1299|5end_MutT/NUDIX_family_protein_1261719..1261949_POSITIVE 268 80 41 187 134 smit:SMT_1130|5end_MATE_efflux_family_protein_1065210..1065440_NEGATIVE 268 97 61 183 150 smit:SMT_976|5end_hypothetical_protein_925183..925413_NEGATIVE 267 64 25 45 1 smit:SMT_1584|5end_methyltransferase,_putative_1574713..1574943_POSITIVE 264 68 26 166 110 smit:SMT_1645|5end_hypothetical_protein_1631738..1631968_POSITIVE 259 109 70 139 99 smit:SMT_1752|5end_N-(5'-phosphoribosyl)-anthranilate_isomerase_1729772..1730002_POSITIVE 256 78 35 168 121 smit:SMT_2105|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1996458..1996688_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 319 90 41 122 61 smit:SMT_896|3end_hypothetical_protein_843119..843269_NEGATIVE 292 49 3 72 28 smit:SMT_936|3end_cobyric_acid_synthase_889531..889681_POSITIVE 292 49 3 72 28 smit:SMT_931|3end_cobyric_acid_synthase_885017..885167_POSITIVE 289 78 35 70 26 smit:SMT_392|3end_acetyltransferase,_GNAT_family_324007..324157_POSITIVE 279 68 21 80 38 smit:SMT_1704|3end_D-alanine-D-alanine_ligase_1688554..1688704_POSITIVE 268 70 26 100 55 smit:SMT_1298|3end_K03811_nicotinamide_mononucleotide_transporter_1261797..1261947_POSITIVE 268 80 41 126 73 smit:SMT_1131|3end_threonine_synthase_1065271..1065421_NEGATIVE 268 97 61 114 81 smit:SMT_977|3end_conserved_hypothetical_protein,_Rrf2_family_protein_925252..925402_NEGATIVE 261 65 21 94 52 smit:SMT_77|3end_inositol-5-monophosphate_dehydrogenase_3876..4026_NEGATIVE 257 45 4 44 10 smit:SMT_1542|3end_phosphomevalonate_kinase_1526077..1526227_NEGATIVE 256 75 34 99 47 smit:SMT_1352|3end_formamidopyrimidine-DNA_glycosylase_1329817..1329967_NEGATIVE 253 90 42 107 57 smit:SMT_356|3end_hypothetical_protein_293268..293418_POSITIVE 252 70 33 100 65 smit:SMT_894|3end_hypothetical_protein_841315..841465_NEGATIVE 244 80 34 59 6 smit:SMT_1584|3end_methyltransferase,_putative_1575332..1575482_POSITIVE 243 99 54 151 91 smit:SMT_395|3end_hypothetical_protein_325027..325177_POSITIVE 241 69 21 107 58 smit:SMT_141|3end_K03495_glucose_inhibited_division_protein_A_93401..93551_POSITIVE 240 69 21 107 53 smit:SMT_1693|3end_dimethyladenosine_transferase_1676752..1676902_POSITIVE 238 55 26 151 113 smit:SMT_1644|3end_3-isopropylmalate_dehydrogenase_1631821..1631971_POSITIVE 238 47 3 82 36 smit:SMT_1172|3end_hypothetical_protein_1119344..1119494_NEGATIVE 236 75 35 68 27 smit:SMT_1181|3end_phosphotransferase_system,_mannose/fructose-specific_component_IIA_1139413..1139563_NEGATIVE