Origin IGS: ctagcaacctctttctaatttaattataacttaaaagttattaaaagtaaatcctaacattcataaaaagaagaccttagaaaaattccaaaggtctcatctttattattcgaaaaaatacgagaagagttataaattgaaggcaatccttacta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tagtaaggattgccttcaatttataactcttctcgtattttttcgaataataaagatgagacctttggaatttttctaaggtcttctttttatgaatgttaggatttacttttaataacttttaagttataattaaattagaaagaggttgctag Mask Tandem Repeat Region ================================================ ctagcaacctctttctaatttaattataacttaaaagttattaaaagtaaatcctaacattcataaaaagaagaccttagaaaaattccaaaggtctcatctttattattcgaaaaaatacgagaagagttataaattgaaggcaatccttacta Find is-nt database================================================ Query_seq: SMT_902:SMT_903|SMT_902:SMT_903:hypothetical protein:hypothetical protein:<-<-:851880..852034 155 ctagcaacctctttctaatttaattataacttaaaagttattaaaagtaaatcctaacattcataaaaagaagaccttagaaaaattccaaaggtctcatctttattattcgaaaaaatacgagaagagttataaattgaaggcaatccttacta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_902:SMT_903|SMT_902:SMT_903:hypothetical protein:hypothetical protein:<-<-:851880..852034 155 ctagcaacctctttctaatttaattataacttaaaagttattaaaagtaaatcctaacattcataaaaagaagaccttagaaaaattccaaaggtctcatctttattattcgaaaaaatacgagaagagttataaattgaaggcaatccttacta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_902:SMT_903|SMT_902:SMT_903:hypothetical protein:hypothetical protein:<-<-:851880..852034 155 ctagcaacctctttctaatttaattataacttaaaagttattaaaagtaaatcctaacattcataaaaagaagaccttagaaaaattccaaaggtctcatctttattattcgaaaaaatacgagaagagttataaattgaaggcaatccttacta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ctagcaacctctttctaatttaattataacttaaaagttattaaaagtaaatcctaacattcataaaaagaagaccttagaaaaattccaaaggtctcatctttattattcgaaaaaatacgagaagagttataaattgaaggcaatccttacta Predict ORF larger than 30AA ================================================ Protein_Len: 32 Strand: + Start: 59 End: 154 .......................................................... M H K K K T L E K F Q R S H L Y Y S K K Y E K S Y K L K A I L T . Protein_Len: 30 Strand: - Start: 57 End: 146 ........................................................ C E Y F S S R L F I G F T E D K N N S F I R S S N Y I S P M ......... ctagcaacctctttctaatttaattataacttaaaagttattaaaagtaaatcctaacattcataaaaagaagaccttagaaaaattccaaaggtctcatctttattattcgaaaaaatacgagaagagttataaattgaaggcaatccttacta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: - Score: 88 Start: 23 End: 52 ......................ATTTACTTTTAATAACTTTTAAGTTATAAT....................................................................................................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ctagcaacctctttctaatttaattataacttaaaagttattaaaagtaaatcctaacattcataaaaagaagaccttagaaaaattccaaaggtctcatctttattattcgaaaaaatacgagaagagttataaattgaaggcaatccttacta Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 71 HP_score: -6.0 Tail_Score: -4.5448 Start: 73 End: 96 Full_Region: gaataataaagatga gacctttgga attt ttctaaggtc ttctttttatgaatg ........................................................................gacctttggaatttttctaaggtc........................................................... Find igs database================================================ Query_seq: SMT_902:SMT_903|SMT_902:SMT_903:hypothetical protein:hypothetical protein:<-<-:851880..852034 155 ctagcaacctctttctaatttaattataacttaaaagttattaaaagtaaatcctaacattcataaaaagaagaccttagaaaaattccaaaggtctcatctttattattcgaaaaaatacgagaagagttataaattgaaggcaatccttacta Intra-Species Hit: Count: 1 Min: 1 Max: 155 Len: 155 Subject: smit_SMT_902_SMT_903|hypothetical protein:hypothetical protein|NEGATIVE:NEGATIVE|[851880,852034]|155 HSP 1 e-value: 6.0E-83 bit: 307.0 Len: 155 Query Start:1 Query End:155 Subject Strand: POSITIVE Subject Start: 1 Subject End: 155 ctagcaacctctttctaatttaattataacttaaaagttattaaaagtaaatcctaacattcataaaaagaagaccttagaaaaattccaaaggtctcatctttattattcgaaaaaatacgagaagagttataaattgaaggcaatccttacta ctagcaacctctttctaatttaattataacttaaaagttattaaaagtaaatcctaacattcataaaaagaagaccttagaaaaattccaaaggtctcatctttattattcgaaaaaatacgagaagagttataaattgaaggcaatccttacta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CUAGCAACCUCUUUCUAAUUUAAUUAUAACUUAAAAGUUAUUAAAAGUAAAUCCUAACAUUCAUAAAAAGAAGACCUUAGAAAAAUUCCAAAGGUCUCAUCUUUAUUAUUCGAAAAAAUACGAGAAGAGUUAUAAAUUGAAGGCAAUCCUUACUA ..........(((((.((((((...((((((....)))))).................((((....((((((((((((.(........).)))))))..)))))....((((........))))..))))..)))))))))))............ (-21.90) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGUAAGGAUUGCCUUCAAUUUAUAACUCUUCUCGUAUUUUUUCGAAUAAUAAAGAUGAGACCUUUGGAAUUUUUCUAAGGUCUUCUUUUUAUGAAUGUUAGGAUUUACUUUUAAUAACUUUUAAGUUAUAAUUAAAUUAGAAAGAGGUUGCUAG (((((.((((((....))))))....(((((((.(((..((((......(((((((.((((((((.(((.....))))))))))).))))))).......))))..))).....((((((....)))))).........)).)))))..))))). (-30.32) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 760 100 51 191 142 smit:SMT_902|5end_hypothetical_protein_851789..852019_NEGATIVE 241 150 110 229 185 smit:SMT_1655|5end_glucose-6-phosphate_1-dehydrogenase_1645021..1645251_NEGATIVE 228 105 70 214 172 smit:SMT_1371|5end_hypothetical_protein_1350794..1351024_NEGATIVE 210 97 52 143 98 smit:SMT_1092|5end_transcription_antitermination_factor_1033696..1033926_NEGATIVE 201 47 9 142 98 smit:SMT_1564|5end_transcriptional_antiterminator,_BglG_family_1555587..1555817_POSITIVE 200 90 44 58 8 smit:SMT_1338|5end_K05832_putative_ABC_transport_system_permease_protein_1313675..1313905_NEGATIVE 196 130 91 55 18 smit:SMT_1395|5end_K07025_putative_hydrolase_of_the_HAD_superfamily_1371310..1371540_POSITIVE 191 108 69 168 124 smit:SMT_1877|5end_K06147_ATP-binding_cassette,_subfamily_B,_bacterial_1834039..1834269_POSITIVE 186 101 61 184 154 smit:SMT_486|5end_hypothetical_protein_422305..422535_NEGATIVE 184 89 53 160 120 smit:SMT_2125|5end_3-phosphoshikimate_1-carboxyvinyltransferase_2008872..2009102_POSITIVE 184 89 53 160 120 smit:SMT_750|5end_3-phosphoshikimate_1-carboxyvinyltransferase_655247..655477_NEGATIVE 184 25 1 161 136 smit:SMT_91|5end_hypothetical_protein_22578..22808_POSITIVE 183 140 95 157 114 smit:SMT_1204|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1164723..1164953_NEGATIVE 181 127 85 207 164 smit:SMT_329|5end_hypothetical_protein_265240..265470_POSITIVE 180 98 63 107 74 smit:SMT_1685|5end_phenylalanyl-tRNA_synthetase_alpha_chain_1668845..1669075_NEGATIVE 179 135 97 128 88 smit:SMT_2028|5end_leader_peptidase_(prepilin_peptidase)_/_N-methyltransferase_1943729..1943959_NEGATIVE 179 135 97 128 88 smit:SMT_1754|5end_leader_peptidase_(prepilin_peptidase)_/_N-methyltransferase_1733022..1733252_NEGATIVE 179 140 99 189 149 smit:SMT_1436|5end_conserved_hypothetical_protein_TIGR00147_1421255..1421485_NEGATIVE 178 150 106 226 188 smit:SMT_1464|5end_K02030_polar_amino_acid_transport_system_substrate-binding_protein_1444721..1444951_POSITIVE 175 131 93 61 19 smit:SMT_1717|5end_DNA-3-methyladenine_glycosylase_I_1704274..1704504_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 241 150 110 78 34 smit:SMT_1656|3end_glutamine_ABC_transporter_ATP-binding_protein_1645172..1645322_NEGATIVE 229 149 111 77 40 smit:SMT_1707|3end_cell_division_protein_FtsA_1691846..1691996_POSITIVE 210 97 52 89 44 smit:SMT_1093|3end_K03073_preprotein_translocase_SecE_subunit_1033750..1033900_NEGATIVE 207 97 52 75 29 smit:SMT_1212|3end_acyl_carrier_protein,_putative_1171424..1171574_NEGATIVE 201 129 92 37 7 smit:SMT_247|3end_K02996_small_subunit_ribosomal_protein_S9_183639..183789_POSITIVE 184 89 53 68 28 smit:SMT_2124|3end_hypothetical_protein_2008860..2009010_POSITIVE 184 89 53 68 28 smit:SMT_751|3end_hypothetical_protein_655339..655489_NEGATIVE 179 140 99 79 39 smit:SMT_1437|3end_ADP-ribosylglycohydrolase_1421365..1421515_NEGATIVE 178 150 106 68 30 smit:SMT_1463|3end_phosphate_transport_system_protein_PhoU_1444643..1444793_POSITIVE 178 126 81 113 50 smit:SMT_1307|3end_glycerol_uptake_facilitator_protein_1272448..1272598_POSITIVE 175 131 93 51 9 smit:SMT_1718|3end_Holliday_junction_DNA_helicase_RuvA_1704284..1704434_NEGATIVE 174 127 81 81 49 smit:SMT_676|3end_DNA_helicase_II_583020..583170_NEGATIVE 173 113 77 101 63 smit:SMT_917|3end_hydroxymethylglutaryl-CoA_synthase_870004..870154_NEGATIVE 172 100 69 94 72 smit:SMT_838|3end_cation_efflux_family_protein_784203..784353_POSITIVE 169 100 56 140 87 smit:SMT_1070|3end_hypothetical_protein_1009644..1009794_NEGATIVE 168 101 70 78 36 smit:SMT_1669|3end_potassium_uptake_protein_KtrB_1658302..1658452_NEGATIVE 168 101 70 78 36 smit:SMT_1168|3end_potassium_uptake_protein_KtrB_1115173..1115323_NEGATIVE 167 97 75 27 4 smit:SMT_228|3end_hypothetical_protein_165093..165243_POSITIVE 167 97 75 27 4 smit:SMT_224|3end_hypothetical_protein_159599..159749_POSITIVE 166 104 77 132 104 smit:SMT_2087|3end_cadmium_efflux_system_accessory_protein_1984108..1984258_POSITIVE