Origin IGS:
ggctgttcttttatttttttatctcacaaggttatccactatttttttcaaaaaaaagcttcataaatcaatagtttctgattttatccccaacctgtggataaaacttgataacattgtggattatttttcacagcttgtggaaaattcttgctatctatggtaaaataaatctagcattaaacttttaaatagtaaaggaggagaaagga
.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9
tcctttctcctcctttactatttaaaagtttaatgctagatttattttaccatagatagcaagaattttccacaagctgtgaaaaataatccacaatgttatcaagttttatccacaggttggggataaaatcagaaactattgatttatgaagcttttttttgaaaaaaatagtggataaccttgtgagataaaaaaataaaagaacagcc
Mask Tandem Repeat Region ================================================
ggctgttcttttatttttttatctcacaaggttatccactatttttttcaaaaaaaagcttcataaatcaatagtttctgattttatccccaacctgtggataaaacttgataacattgtggattatttttcacagcttgtggaaaattcttgctatctatggtaaaataaatctagcattaaacttttaaatagtaaaggaggagaaagga
Find is-nt database================================================
Query_seq: SMT_88:SMT_89|SMT_88:SMT_89:K03497 chromosome partitioning protein, ParB family:chromosomal replication initiator protein:->->:19785..19996 212
ggctgttcttttatttttttatctcacaaggttatccactatttttttcaaaaaaaagcttcataaatcaatagtttctgattttatccccaacctgtggataaaacttgataacattgtggattatttttcacagcttgtggaaaattcttgctatctatggtaaaataaatctagcattaaacttttaaatagtaaaggaggagaaagga
Intra-Species Hit: Count: 0
Inter-species Hit: Count: 0
Find is-aa database================================================
Query_seq: SMT_88:SMT_89|SMT_88:SMT_89:K03497 chromosome partitioning protein, ParB family:chromosomal replication initiator protein:->->:19785..19996 212
ggctgttcttttatttttttatctcacaaggttatccactatttttttcaaaaaaaagcttcataaatcaatagtttctgattttatccccaacctgtggataaaacttgataacattgtggattatttttcacagcttgtggaaaattcttgctatctatggtaaaataaatctagcattaaacttttaaatagtaaaggaggagaaagga
Intra-Species Hit: Count: 0
Inter-species Hit: Count: 0
Find nr database================================================
Query_seq: SMT_88:SMT_89|SMT_88:SMT_89:K03497 chromosome partitioning protein, ParB family:chromosomal replication initiator protein:->->:19785..19996 212
ggctgttcttttatttttttatctcacaaggttatccactatttttttcaaaaaaaagcttcataaatcaatagtttctgattttatccccaacctgtggataaaacttgataacattgtggattatttttcacagcttgtggaaaattcttgctatctatggtaaaataaatctagcattaaacttttaaatagtaaaggaggagaaagga
Intra-Species Hit: Count: 0
Inter-species Hit: Count: 0
ggctgttcttttatttttttatctcacaaggttatccactatttttttcaaaaaaaagcttcataaatcaatagtttctgattttatccccaacctgtggataaaacttgataacattgtggattatttttcacagcttgtggaaaattcttgctatctatggtaaaataaatctagcattaaacttttaaatagtaaaggaggagaaagga
Predict ORF larger than 30AA ================================================
Protein_Len: 41 Strand: + Start: 41 End: 163
........................................ M F F K K K L H K S I V S D F I P N L W I K L D N I V D Y F S Q L V E N S C Y L W .................................................
ggctgttcttttatttttttatctcacaaggttatccactatttttttcaaaaaaaagcttcataaatcaatagtttctgattttatccccaacctgtggataaaacttgataacattgtggattatttttcacagcttgtggaaaattcttgctatctatggtaaaataaatctagcattaaacttttaaatagtaaaggaggagaaagga
Predict Promoter with matrix: RpoD-15 score > 80================================================
Predict Promoter with matrix: RpoD-16 score > 80================================================
Predict Promoter with matrix: RpoD-17 score > 80================================================
PromScan Matrix: RpoD-17 Strand: + Score: 84 Start: 140 End: 169
...........................................................................................................................................GTGGAAAATTCTTGCTATCTATGGTAAAAT...........................................
Predict Promoter with matrix: RpoD-18 score > 80================================================
Predict Promoter with matrix: RpoD-19 score > 80================================================
Predict Promoter with matrix: RpoN score > 80================================================
ggctgttcttttatttttttatctcacaaggttatccactatttttttcaaaaaaaagcttcataaatcaatagtttctgattttatccccaacctgtggataaaacttgataacattgtggattatttttcacagcttgtggaaaattcttgctatctatggtaaaataaatctagcattaaacttttaaatagtaaaggaggagaaagga
Predict TransTerm conf > 70================================================
TransTerm Strand: + Conf: 70 HP_score: -8.7 Tail_Score: -2.51471 Start: 82 End: 106 Full_Region: ATCAATAGTTTCTGA TTTTATCCCCA ACC TGTGGATAAAA CTTGATAACATTGTG
.................................................................................TTTTATCCCCAACCTGTGGATAAAA..........................................................................................................
TransTerm Strand: + Conf: 73 HP_score: -7.7 Tail_Score: -3.81089 Start: 127 End: 147 Full_Region: TAACATTGTGGATTA TTTTTCACA GCT TGTGGAAAA TTCTTGCTATCTATG
..............................................................................................................................TTTTTCACAGCTTGTGGAAAA.................................................................
Find igs database================================================
Query_seq: SMT_88:SMT_89|SMT_88:SMT_89:K03497 chromosome partitioning protein, ParB family:chromosomal replication initiator protein:->->:19785..19996 212
ggctgttcttttatttttttatctcacaaggttatccactatttttttcaaaaaaaagcttcataaatcaatagtttctgattttatccccaacctgtggataaaacttgataacattgtggattatttttcacagcttgtggaaaattcttgctatctatggtaaaataaatctagcattaaacttttaaatagtaaaggaggagaaagga
Intra-Species Hit: Count: 1 Min: 58 Max: 212 Len: 155
Subject: smit_SMT_88_SMT_89|K03497 chromosome partitioning protein, ParB family:chromosomal replication initiator protein|POSITIVE:POSITIVE|[19785,19996]|212
HSP 1 e-value: 8.0E-83 bit: 307.0 Len: 155 Query Start:58 Query End:212 Subject Strand: POSITIVE Subject Start: 58 Subject End: 212
.........................................................gcttcataaatcaatagtttctgattttatccccaacctgtggataaaacttgataacattgtggattatttttcacagcttgtggaaaattcttgctatctatggtaaaataaatctagcattaaacttttaaatagtaaaggaggagaaagga
.........................................................gcttcataaatcaatagtttctgattttatccccaacctgtggataaaacttgataacattgtggattatttttcacagcttgtggaaaattcttgctatctatggtaaaataaatctagcattaaacttttaaatagtaaaggaggagaaagga
Inter-species Hit: Count: 3 Min: 58 Max: 212 Len: 155
Subject: NC_008533_SPD_2069_SPD_0001|SpoJ protein:chromosomal replication initiator protein DnaA|POSITIVE:POSITIVE|[2045904,2046115]|212
HSP 1 e-value: 6.0E-59 bit: 228.0 Len: 155 Query Start:58 Query End:212 Subject Strand: POSITIVE Subject Start: 58 Subject End: 212
.........................................................gcttcataaatcaatagtttctgattttatccccaacctgtggataaaacttgataacattgtggattatttttcacagcttgtggaaaattcttgctatctatggtaaaataaatctagcattaaacttttaaatagtaaaggaggagaaagga
.........................................................gcttaataaatcaataatttcttcttttatccccaacctgtggataaagtttggtaacattgtggattatttttcacagcttgtggaaaattcttgctatctatggtaaaatatctctagtattaaacttttaaatagtaaaggaggagaaagga
Subject: NC_003098_spr2046_spr0001|Chromosome segregation protein:chromosomal replication initiation protein|POSITIVE:POSITIVE|[2038404,2038615]|212
HSP 1 e-value: 6.0E-59 bit: 228.0 Len: 155 Query Start:58 Query End:212 Subject Strand: POSITIVE Subject Start: 58 Subject End: 212
.........................................................gcttcataaatcaatagtttctgattttatccccaacctgtggataaaacttgataacattgtggattatttttcacagcttgtggaaaattcttgctatctatggtaaaataaatctagcattaaacttttaaatagtaaaggaggagaaagga
.........................................................gcttaataaatcaataatttcttcttttatccccaacctgtggataaagtttggtaacattgtggattatttttcacagcttgtggaaaattcttgctatctatggtaaaatatctctagtattaaacttttaaatagtaaaggaggagaaagga
Subject: NC_003028_SP_2240_SP_0001|spspoJ protein:chromosomal replication initiation protein|POSITIVE:POSITIVE|2160827, 196 {([2160827,2160842]), ([1,196])} (circular)|212
HSP 1 e-value: 6.0E-59 bit: 228.0 Len: 155 Query Start:58 Query End:212 Subject Strand: POSITIVE Subject Start: 58 Subject End: 212
.........................................................gcttcataaatcaatagtttctgattttatccccaacctgtggataaaacttgataacattgtggattatttttcacagcttgtggaaaattcttgctatctatggtaaaataaatctagcattaaacttttaaatagtaaaggaggagaaagga
.........................................................gcttaataaatcaataatttcttcttttatccccaacctgtggataaagtttggtaacattgtggattatttttcacagcttgtggaaaattcttgctatctatggtaaaatatctctagtattaaacttttaaatagtaaaggaggagaaagga
Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================
GCUUCAUAAAUCAAUAGUUUCUGAUUUUAUCCCCAACCUGUGGAUAAAACUUGAUAACAUUGUGGAUUAUUUUUCACAGCUUGUGGAAAAUUCUUGCUAUCUAUGGUAAAAUAAAUCUAGCAUUAAACUUUUAAAUAGUAAAGGAGGAGAAAGGA
.((((......((((.(((((...((((((((.(.....).))))))))...)).)))))))((((((.((((((((.....))))))))...((((((....))))))....))))))........((((((.....)))))).))))...... (-27.00)
Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================
UCCUUUCUCCUCCUUUACUAUUUAAAAGUUUAAUGCUAGAUUUAUUUUACCAUAGAUAGCAAGAAUUUUCCACAAGCUGUGAAAAAUAAUCCACAAUGUUAUCAAGUUUUAUCCACAGGUUGGGGAUAAAAUCAGAAACUAUUGAUUUAUGAAGC
............(((((..(.((((.(((((..(((((..(((((......)))))))))).((.((((((.(((.(((((....((((..(.............)..)))).))))).))).))).))).))..))))).)))).)..))))). (-24.72)
Find mRNA Target Using Conserved IGS================================================
5'END mRNA Target Prediction===================================
Score srna_start srna_end target_start tegart_end seq_id
294 59 14 67 30 smit:SMT_1474|5end_K02003_1455170..1455400_POSITIVE
258 53 12 58 5 smit:SMT_982|5end_D-tyrosyl-tRNA(Tyr)_deacylase_929654..929884_NEGATIVE
249 68 30 130 97 smit:SMT_2023|5end_tryptophan_synthase,_alpha_subunit_1939315..1939545_POSITIVE
249 68 30 130 97 smit:SMT_1818|5end_tryptophan_synthase,_alpha_subunit_1792190..1792420_POSITIVE
248 109 63 156 106 smit:SMT_1897|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1848858..1849088_NEGATIVE
245 40 1 226 181 smit:SMT_395|5end_hypothetical_protein_324459..324689_POSITIVE
242 47 2 165 128 smit:SMT_1794|5end_SOS_responce_UmuC_protein_1769074..1769304_NEGATIVE
242 47 2 193 156 smit:SMT_1066|5end_holo-(acyl_carrier_protein)_synthase_1005411..1005641_POSITIVE
242 107 62 48 4 smit:SMT_669|5end_ribose-phosphate_pyrophosphokinase_578160..578390_POSITIVE
235 65 36 44 14 smit:SMT_957|5end_UDP-glucose_4-epimerase_905003..905233_NEGATIVE
235 65 36 44 14 smit:SMT_950|5end_UDP-glucose_4-epimerase_898696..898926_NEGATIVE
212 45 1 73 21 smit:SMT_1428|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1414622..1414852_NEGATIVE
208 90 41 210 159 smit:SMT_1123|5end_hypothetical_protein_1059292..1059522_POSITIVE
205 103 73 220 185 smit:SMT_2147|5end_hypothetical_protein_2022895..2023125_NEGATIVE
205 80 42 89 45 smit:SMT_273|5end_glucan_1,6-alpha-glucosidase_213418..213648_POSITIVE
204 108 61 52 15 smit:SMT_1432|5end_hypothetical_protein_1417468..1417698_NEGATIVE
204 40 1 123 84 smit:SMT_229|5end_mechanosensitive_ion_channel_165623..165853_NEGATIVE
203 122 90 90 47 smit:SMT_2002|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1924405..1924635_NEGATIVE
203 120 71 130 85 smit:SMT_1221|5end_choline_binding_protein_A_1185539..1185769_NEGATIVE
198 66 30 42 4 smit:SMT_1632|5end_hypothetical_protein_1619060..1619290_NEGATIVE
3'END mRNA Target Prediction===================================
Score srna_start srna_end target_start tegart_end seq_id
248 109 63 137 87 smit:SMT_1898|3end_aminotransferase_(class_V),_putative_1848877..1849027_NEGATIVE
242 47 2 54 17 smit:SMT_1795|3end_DNA_polymerase_III,_delta_subunit_1769185..1769335_NEGATIVE
240 102 61 59 17 smit:SMT_2015|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1932839..1932989_POSITIVE
209 39 2 148 117 smit:SMT_1065|3end_phospho-2-dehydro-3-deoxyheptonate_aldolase_1005452..1005602_POSITIVE
208 74 38 60 23 smit:SMT_77|3end_inositol-5-monophosphate_dehydrogenase_3876..4026_NEGATIVE
204 108 61 48 11 smit:SMT_1433|3end_hypothetical_protein_1417472..1417622_NEGATIVE
204 71 33 92 53 smit:SMT_763|3end_alpha-amylase_669280..669430_POSITIVE
204 40 1 80 41 smit:SMT_230|3end_hypothetical_protein_predicted_by_Glimmer/Critica_165666..165816_NEGATIVE
195 41 6 127 86 smit:SMT_1061|3end_hypothetical_protein_999915..1000065_POSITIVE
195 86 62 53 21 smit:SMT_762|3end_cation-transporting_ATPase,_E1-E2_family_protein_667368..667518_NEGATIVE
195 45 2 143 97 smit:SMT_260|3end_phage_transcriptional_repressor_200236..200386_POSITIVE
194 75 33 87 41 smit:SMT_1359|3end_dihydroorotate_dehydrogenase_electron_transfer_subunit_1337203..1337353_NEGATIVE
193 76 31 73 25 smit:SMT_1268|3end_acetoin_reductase_1232655..1232805_NEGATIVE
190 106 63 110 58 smit:SMT_2056|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1966735..1966885_POSITIVE
190 102 61 73 31 smit:SMT_2023|3end_tryptophan_synthase,_alpha_subunit_1939997..1940147_POSITIVE
190 102 61 60 18 smit:SMT_1674|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1660361..1660511_NEGATIVE
190 107 76 78 43 smit:SMT_1670|3end_K02030_polar_amino_acid_transport_system_substrate-binding_protein_1659295..1659445_POSITIVE
190 107 76 57 22 smit:SMT_1068|3end_ATP-dependent_DNA_helicase_RecG_1008986..1009136_POSITIVE
190 107 76 40 5 smit:SMT_916|3end_3-hydroxy-3-methylglutaryl-CoA_reductase_868730..868880_NEGATIVE
189 147 104 53 2 smit:SMT_1805|3end_2,3,4,5-tetrahydropyridine-2-carboxylate_N-succinyltransferase,_putative_1781241..1781391_POSITIVE