Origin IGS: ctcttactctctttccaacaataatagaatttaactactattctactatttttgtttagaaagtctagtaaaaacatattttagtttctattccatagaagacatggttattgaaagagtaatacaaactaaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tttagtttgtattactctttcaataaccatgtcttctatggaatagaaactaaaatatgtttttactagactttctaaacaaaaatagtagaatagtagttaaattctattattgttggaaagagagtaagag Mask Tandem Repeat Region ================================================ ctcttactctctttccaacaataatagaatttaactactattctactatttttgtttagaaagtctagtaaaaacatattttagtttctattccatagaagacatggttattgaaagagtaatacaaactaaa Find is-nt database================================================ Query_seq: SMT_881:SMT_882|SMT_881:SMT_882:peptidoglycan branched peptide synthesis protein, alanine adding enzyme:acetyl esterase:<-<-:829619..829751 133 ctcttactctctttccaacaataatagaatttaactactattctactatttttgtttagaaagtctagtaaaaacatattttagtttctattccatagaagacatggttattgaaagagtaatacaaactaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_881:SMT_882|SMT_881:SMT_882:peptidoglycan branched peptide synthesis protein, alanine adding enzyme:acetyl esterase:<-<-:829619..829751 133 ctcttactctctttccaacaataatagaatttaactactattctactatttttgtttagaaagtctagtaaaaacatattttagtttctattccatagaagacatggttattgaaagagtaatacaaactaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_881:SMT_882|SMT_881:SMT_882:peptidoglycan branched peptide synthesis protein, alanine adding enzyme:acetyl esterase:<-<-:829619..829751 133 ctcttactctctttccaacaataatagaatttaactactattctactatttttgtttagaaagtctagtaaaaacatattttagtttctattccatagaagacatggttattgaaagagtaatacaaactaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ctcttactctctttccaacaataatagaatttaactactattctactatttttgtttagaaagtctagtaaaaacatattttagtttctattccatagaagacatggttattgaaagagtaatacaaactaaa Predict ORF larger than 30AA ================================================ ctcttactctctttccaacaataatagaatttaactactattctactatttttgtttagaaagtctagtaaaaacatattttagtttctattccatagaagacatggttattgaaagagtaatacaaactaaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ctcttactctctttccaacaataatagaatttaactactattctactatttttgtttagaaagtctagtaaaaacatattttagtttctattccatagaagacatggttattgaaagagtaatacaaactaaa Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: SMT_881:SMT_882|SMT_881:SMT_882:peptidoglycan branched peptide synthesis protein, alanine adding enzyme:acetyl esterase:<-<-:829619..829751 133 ctcttactctctttccaacaataatagaatttaactactattctactatttttgtttagaaagtctagtaaaaacatattttagtttctattccatagaagacatggttattgaaagagtaatacaaactaaa Intra-Species Hit: Count: 1 Min: 1 Max: 133 Len: 133 Subject: smit_SMT_881_SMT_882|peptidoglycan branched peptide synthesis protein, alanine adding enzyme:acetyl esterase|NEGATIVE:NEGATIVE|[829619,829751]|133 HSP 1 e-value: 7.0E-70 bit: 264.0 Len: 133 Query Start:1 Query End:133 Subject Strand: POSITIVE Subject Start: 1 Subject End: 133 ctcttactctctttccaacaataatagaatttaactactattctactatttttgtttagaaagtctagtaaaaacatattttagtttctattccatagaagacatggttattgaaagagtaatacaaactaaa ctcttactctctttccaacaataatagaatttaactactattctactatttttgtttagaaagtctagtaaaaacatattttagtttctattccatagaagacatggttattgaaagagtaatacaaactaaa Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CUCUUACUCUCUUUCCAACAAUAAUAGAAUUUAACUACUAUUCUACUAUUUUUGUUUAGAAAGUCUAGUAAAAACAUAUUUUAGUUUCUAUUCCAUAGAAGACAUGGUUAUUGAAAGAGUAAUACAAACUAAA ...(((((((((((((((.((((.((((((.........)))))).)))).)))....)))))..((((((...(((.......(((((((...)))))))..))).))))))..)))))))........... (-19.40) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUUAGUUUGUAUUACUCUUUCAAUAACCAUGUCUUCUAUGGAAUAGAAACUAAAAUAUGUUUUUACUAGACUUUCUAAACAAAAAUAGUAGAAUAGUAGUUAAAUUCUAUUAUUGUUGGAAAGAGAGUAAGAG ((((((((.((((.............(((((.....))))))))).))))))))......(((((((...((((((((.....(((((((((((.........))))))))))).))))))))..))))))). (-31.01) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 743 130 81 221 172 smit:SMT_881|5end_peptidoglycan_branched_peptide_synthesis_protein,_alanine_adding_enzyme_829528..829758_NEGATIVE 235 128 83 181 122 smit:SMT_2159|5end_cell_division_protein_FtsA_2032010..2032240_POSITIVE 213 121 83 173 130 smit:SMT_352|5end_hypothetical_protein_290531..290761_POSITIVE 207 128 83 181 122 smit:SMT_2163|5end_cell_division_protein_FtsA_2037643..2037873_POSITIVE 207 128 83 181 122 smit:SMT_2160|5end_cell_division_protein_FtsA_2033524..2033754_POSITIVE 207 128 83 181 122 smit:SMT_2158|5end_cell_division_protein_2031534..2031764_POSITIVE 207 128 83 181 122 smit:SMT_1707|5end_cell_division_protein_FtsA_1690945..1691175_POSITIVE 207 128 83 181 122 smit:SMT_1547|5end_cell_division_protein_FtsA_1530987..1531217_POSITIVE 206 50 1 224 181 smit:SMT_2024|5end_hypothetical_protein_1940359..1940589_POSITIVE 206 50 1 224 181 smit:SMT_724|5end_hypothetical_protein_630060..630290_NEGATIVE 193 118 82 231 196 smit:SMT_136|5end_hypothetical_protein_predicted_by_Glimmer/Critica_87314..87544_POSITIVE 189 98 51 195 152 smit:SMT_1194|5end_phosphoribosylformylglycinamidine_cyclo-ligase_1153171..1153401_NEGATIVE 188 109 64 79 35 smit:SMT_825|5end_aspartate_aminotransferase_749594..749824_NEGATIVE 184 99 51 107 56 smit:SMT_1405|5end_tyrosine_recombinase_1383150..1383380_NEGATIVE 180 90 41 104 56 smit:SMT_218|5end_undecaprenyl_pyrophosphate_synthetase_150155..150385_POSITIVE 179 120 75 144 87 smit:SMT_2023|5end_tryptophan_synthase,_alpha_subunit_1939315..1939545_POSITIVE 179 120 75 144 87 smit:SMT_1818|5end_tryptophan_synthase,_alpha_subunit_1792190..1792420_POSITIVE 179 126 83 80 37 smit:SMT_1233|5end_cadmium_resistance_protein_1194306..1194536_POSITIVE 179 54 11 136 94 smit:SMT_356|5end_hypothetical_protein_292394..292624_POSITIVE 179 90 43 60 10 smit:SMT_293|5end_dTDP-4-dehydrorhamnose_reductase_235919..236149_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 262 77 36 57 15 smit:SMT_433|3end_cell_division_protein_DivIB_375954..376104_POSITIVE 234 107 62 73 30 smit:SMT_1597|3end_K02032_peptide/nickel_transport_system_ATP-binding_protein_1587987..1588137_POSITIVE 231 69 39 85 52 smit:SMT_1172|3end_hypothetical_protein_1119344..1119494_NEGATIVE 231 47 4 95 45 smit:SMT_773|3end_UDP-N-acetylenolpyruvoylglucosamine_reductase_683460..683610_NEGATIVE 218 120 74 120 78 smit:SMT_693|3end_hypothetical_protein_604078..604228_POSITIVE 188 109 64 82 38 smit:SMT_826|3end_hypothetical_protein_749591..749741_NEGATIVE 184 44 2 79 34 smit:SMT_437|3end_transcriptional_regulator,_TetR_family_379042..379192_POSITIVE 181 130 83 109 49 smit:SMT_1591|3end_DNA-entry_nuclease_1579468..1579618_POSITIVE 180 68 21 144 109 smit:SMT_135|3end_hypothetical_protein_86178..86328_NEGATIVE 174 115 83 151 115 smit:SMT_351|3end_hypothetical_protein_290596..290746_POSITIVE 173 109 63 142 77 smit:SMT_1748|3end_anthranilate_synthase_component_I_1727517..1727667_POSITIVE 171 47 9 43 10 smit:SMT_593|3end_K02030_polar_amino_acid_transport_system_substrate-binding_protein_510317..510467_POSITIVE 171 109 61 149 93 smit:SMT_501|3end_ABC_transporter,_ATP-binding_protein_434848..434998_POSITIVE 170 55 16 101 59 smit:SMT_1696|3end_hypothetical_protein_1678924..1679074_POSITIVE 169 110 63 68 25 smit:SMT_399|3end_cytochrome_c-type_biogenesis_protein_CcdA_329156..329306_POSITIVE 167 110 62 66 23 smit:SMT_702|3end_hypothetical_protein_611946..612096_NEGATIVE 166 110 84 131 105 smit:SMT_1192|3end_phosphoribosylaminoimidazolecarboxamide_formyltransferase_/_IMP_cyclohydrolase_1149859..1150009_NEGATIVE 165 107 61 83 32 smit:SMT_1327|3end_K03402_transcriptional_regulator_of_arginine_metabolism_1304296..1304446_POSITIVE 165 71 34 140 98 smit:SMT_1107|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1047989..1048139_NEGATIVE 165 117 90 100 68 smit:SMT_735|3end_putative_transposase,_ISSmu1_641395..641545_POSITIVE