Origin IGS: aaaaatgtccccccttaaaaatagtgttatagagttacaaagtactgataggtattttcattttctaagagttacaacgcgtaagaaataaaaacgatacttttttatttctgaagctagtaaggcgcatagggtgaccgcattatagcggcaaccgtagaatgacgaacgacttttgagccgtcattctttgcgagttgcgactcgaaagaaaaaatagaaatttctaccatcttatcgggactatataactttaccattataccatttttaaactcaatttgcagtctataatgtagtgtttctgtatagatagttcgttctcataaaacaaaaaggatttaatcctgttatatacagaattaaatcaaaatctgaattgtttttaaattttttatactctctaaaaacacctctataatacatactcagacctaatatagcaataaatttatgaataatagtatttgatttgaataaaatgttaatgactatcccagtcattataaaactaattgcaattatcaaacgctgcttcatttgtacctcaactttctatttcgtagaaaaaaattcagttcctatccaaaagcctataatagctccaccatatcccc .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ggggatatggtggagctattataggcttttggataggaactgaatttttttctacgaaatagaaagttgaggtacaaatgaagcagcgtttgataattgcaattagttttataatgactgggatagtcattaacattttattcaaatcaaatactattattcataaatttattgctatattaggtctgagtatgtattatagaggtgtttttagagagtataaaaaatttaaaaacaattcagattttgatttaattctgtatataacaggattaaatcctttttgttttatgagaacgaactatctatacagaaacactacattatagactgcaaattgagtttaaaaatggtataatggtaaagttatatagtcccgataagatggtagaaatttctattttttctttcgagtcgcaactcgcaaagaatgacggctcaaaagtcgttcgtcattctacggttgccgctataatgcggtcaccctatgcgccttactagcttcagaaataaaaaagtatcgtttttatttcttacgcgttgtaactcttagaaaatgaaaatacctatcagtactttgtaactctataacactatttttaaggggggacattttt Mask Tandem Repeat Region ================================================ aaaaatgtccccccttaaaaatagtgttatagagttacaaagtactgataggtattttcattttctaagagttacaacgcgtaagaaataaaaacgatacttttttatttctgaagctagtaaggcgcatagggtgaccgcattatagcggcaaccgtagaatgacgaacgacttttgagccgtcattctttgcgagttgcgactcgaaagaaaaaatagaaatttctaccatcttatcgggactatataactttaccattataccatttttaaactcaatttgcagtctataatgtagtgtttctgtatagatagttcgttctcataaaacaaaaaggatttaatcctgttatatacagaattaaatcaaaatctgaattgtttttaaattttttatactctctaaaaacacctctataatacatactcagacctaatatagcaataaatttatgaataatagtatttgatttgaataaaatgttaatgactatcccagtcattataaaactaattgcaattatcaaacgctgcttcatttgtacctcaactttctatttcgtagaaaaaaattcagttcctatccaaaagcctataatagctccaccatatcccc Find is-nt database================================================ Query_seq: SMT_804:SMT_805|SMT_804:SMT_805:S-adenosylmethionine synthetase:hypothetical protein predicted by Glimmer/Critica:<-<-:715165..715781 617 aaaaatgtccccccttaaaaatagtgttatagagttacaaagtactgataggtattttcattttctaagagttacaacgcgtaagaaataaaaacgatacttttttatttctgaagctagtaaggcgcatagggtgaccgcattatagcggcaaccgtagaatgacgaacgacttttgagccgtcattctttgcgagttgcgactcgaaagaaaaaatagaaatttctaccatcttatcgggactatataactttaccattataccatttttaaactcaatttgcagtctataatgtagtgtttctgtatagatagttcgttctcataaaacaaaaaggatttaatcctgttatatacagaattaaatcaaaatctgaattgtttttaaattttttatactctctaaaaacacctctataatacatactcagacctaatatagcaataaatttatgaataatagtatttgatttgaataaaatgttaatgactatcccagtcattataaaactaattgcaattatcaaacgctgcttcatttgtacctcaactttctatttcgtagaaaaaaattcagttcctatccaaaagcctataatagctccaccatatcccc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_804:SMT_805|SMT_804:SMT_805:S-adenosylmethionine synthetase:hypothetical protein predicted by Glimmer/Critica:<-<-:715165..715781 617 aaaaatgtccccccttaaaaatagtgttatagagttacaaagtactgataggtattttcattttctaagagttacaacgcgtaagaaataaaaacgatacttttttatttctgaagctagtaaggcgcatagggtgaccgcattatagcggcaaccgtagaatgacgaacgacttttgagccgtcattctttgcgagttgcgactcgaaagaaaaaatagaaatttctaccatcttatcgggactatataactttaccattataccatttttaaactcaatttgcagtctataatgtagtgtttctgtatagatagttcgttctcataaaacaaaaaggatttaatcctgttatatacagaattaaatcaaaatctgaattgtttttaaattttttatactctctaaaaacacctctataatacatactcagacctaatatagcaataaatttatgaataatagtatttgatttgaataaaatgttaatgactatcccagtcattataaaactaattgcaattatcaaacgctgcttcatttgtacctcaactttctatttcgtagaaaaaaattcagttcctatccaaaagcctataatagctccaccatatcccc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_804:SMT_805|SMT_804:SMT_805:S-adenosylmethionine synthetase:hypothetical protein predicted by Glimmer/Critica:<-<-:715165..715781 617 aaaaatgtccccccttaaaaatagtgttatagagttacaaagtactgataggtattttcattttctaagagttacaacgcgtaagaaataaaaacgatacttttttatttctgaagctagtaaggcgcatagggtgaccgcattatagcggcaaccgtagaatgacgaacgacttttgagccgtcattctttgcgagttgcgactcgaaagaaaaaatagaaatttctaccatcttatcgggactatataactttaccattataccatttttaaactcaatttgcagtctataatgtagtgtttctgtatagatagttcgttctcataaaacaaaaaggatttaatcctgttatatacagaattaaatcaaaatctgaattgtttttaaattttttatactctctaaaaacacctctataatacatactcagacctaatatagcaataaatttatgaataatagtatttgatttgaataaaatgttaatgactatcccagtcattataaaactaattgcaattatcaaacgctgcttcatttgtacctcaactttctatttcgtagaaaaaaattcagttcctatccaaaagcctataatagctccaccatatcccc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aaaaatgtccccccttaaaaatagtgttatagagttacaaagtactgataggtattttcattttctaagagttacaacgcgtaagaaataaaaacgatacttttttatttctgaagctagtaaggcgcatagggtgaccgcattatagcggcaaccgtagaatgacgaacgacttttgagccgtcattctttgcgagttgcgactcgaaagaaaaaatagaaatttctaccatcttatcgggactatataactttaccattataccatttttaaactcaatttgcagtctataatgtagtgtttctgtatagatagttcgttctcataaaacaaaaaggatttaatcctgttatatacagaattaaatcaaaatctgaattgtttttaaattttttatactctctaaaaacacctctataatacatactcagacctaatatagcaataaatttatgaataatagtatttgatttgaataaaatgttaatgactatcccagtcattataaaactaattgcaattatcaaacgctgcttcatttgtacctcaactttctatttcgtagaaaaaaattcagttcctatccaaaagcctataatagctccaccatatcccc Predict ORF larger than 30AA ================================================ Protein_Len: 31 Strand: + Start: 326 End: 418 ..................................................................................................................................................................................................................................................................................................................................... M K Q K G F N P V I Y R I K S K S E L F L N F L Y S L K T P L ....................................................................................................................................................................................................... Protein_Len: 53 Strand: + Start: 458 End: 616 ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M I V F D L N K M L M T I P V I I K L I A I I K R C F I C T S T F Y F V E K N S V P I Q K P I I A P P Y P . Protein_Len: 60 Strand: - Start: 292 End: 540 ................................................................................................................................................................................................................................................................................................... I L D F D S N N K F K K Y E R F V G R Y Y M S L G L I A I F K H I I T N S K F L I N I V I G T M I F S I A I I L R Q K M ............................................................................. Protein_Len: 60 Strand: - Start: 120 End: 314 ....................................................................................................................... S R M I A A V T S H R V V K S G D N K A L Q S E F S F I S I E V M K D P S Y L K V M I G N K F E I Q L R Y H L T E T Y M ............................................................................................................................................................................................................................................................................................................... aaaaatgtccccccttaaaaatagtgttatagagttacaaagtactgataggtattttcattttctaagagttacaacgcgtaagaaataaaaacgatacttttttatttctgaagctagtaaggcgcatagggtgaccgcattatagcggcaaccgtagaatgacgaacgacttttgagccgtcattctttgcgagttgcgactcgaaagaaaaaatagaaatttctaccatcttatcgggactatataactttaccattataccatttttaaactcaatttgcagtctataatgtagtgtttctgtatagatagttcgttctcataaaacaaaaaggatttaatcctgttatatacagaattaaatcaaaatctgaattgtttttaaattttttatactctctaaaaacacctctataatacatactcagacctaatatagcaataaatttatgaataatagtatttgatttgaataaaatgttaatgactatcccagtcattataaaactaattgcaattatcaaacgctgcttcatttgtacctcaactttctatttcgtagaaaaaaattcagttcctatccaaaagcctataatagctccaccatatcccc Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 84 Start: 393 End: 422 ........................................................................................................................................................................................................................................................................................................................................................................................................TTTTATACTCTCTAAAAACACCTCTATAAT................................................................................................................................................................................................... PromScan Matrix: RpoD-17 Strand: - Score: 83 Start: 259 End: 288 ..................................................................................................................................................................................................................................................................ACTGCAAATTGAGTTTAAAAATGGTATAAT......................................................................................................................................................................................................................................................................................................................................... PromScan Matrix: RpoD-17 Strand: - Score: 83 Start: 418 End: 447 .................................................................................................................................................................................................................................................................................................................................................................................................................................ATTGCTATATTAGGTCTGAGTATGTATTAT.......................................................................................................................................................................... PromScan Matrix: RpoD-17 Strand: - Score: 81 Start: 362 End: 391 .........................................................................................................................................................................................................................................................................................................................................................................ATTTAAAAACAATTCAGATTTTGATTTAAT.................................................................................................................................................................................................................................. Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aaaaatgtccccccttaaaaatagtgttatagagttacaaagtactgataggtattttcattttctaagagttacaacgcgtaagaaataaaaacgatacttttttatttctgaagctagtaaggcgcatagggtgaccgcattatagcggcaaccgtagaatgacgaacgacttttgagccgtcattctttgcgagttgcgactcgaaagaaaaaatagaaatttctaccatcttatcgggactatataactttaccattataccatttttaaactcaatttgcagtctataatgtagtgtttctgtatagatagttcgttctcataaaacaaaaaggatttaatcctgttatatacagaattaaatcaaaatctgaattgtttttaaattttttatactctctaaaaacacctctataatacatactcagacctaatatagcaataaatttatgaataatagtatttgatttgaataaaatgttaatgactatcccagtcattataaaactaattgcaattatcaaacgctgcttcatttgtacctcaactttctatttcgtagaaaaaaattcagttcctatccaaaagcctataatagctccaccatatcccc Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 78 HP_score: -3.0 Tail_Score: -5.64421 Start: 218 End: 229 Full_Region: gtcccgataagatgg taga aatt tcta ttttttctttcgagt .........................................................................................................................................................................................................................tagaaatttcta.................................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: - Conf: 90 HP_score: -7.2 Tail_Score: -5.54907 Start: 338 End: 370 Full_Region: aaacaattcagattt tgatttaattctgt atata acaggattaaatcc tttttgttttatgag .................................................................................................................................................................................................................................................................................................................................................tgatttaattctgtatataacaggattaaatcc....................................................................................................................................................................................................................................................... TransTerm Strand: - Conf: 95 HP_score: -10.7 Tail_Score: -3.91317 Start: 339 End: 369 Full_Region: aacaattcagatttt gatttaattctgt atata acaggattaaatc ctttttgttttatga ..................................................................................................................................................................................................................................................................................................................................................gatttaattctgtatataacaggattaaatc........................................................................................................................................................................................................................................................ Find igs database================================================ Query_seq: SMT_804:SMT_805|SMT_804:SMT_805:S-adenosylmethionine synthetase:hypothetical protein predicted by Glimmer/Critica:<-<-:715165..715781 617 aaaaatgtccccccttaaaaatagtgttatagagttacaaagtactgataggtattttcattttctaagagttacaacgcgtaagaaataaaaacgatacttttttatttctgaagctagtaaggcgcatagggtgaccgcattatagcggcaaccgtagaatgacgaacgacttttgagccgtcattctttgcgagttgcgactcgaaagaaaaaatagaaatttctaccatcttatcgggactatataactttaccattataccatttttaaactcaatttgcagtctataatgtagtgtttctgtatagatagttcgttctcataaaacaaaaaggatttaatcctgttatatacagaattaaatcaaaatctgaattgtttttaaattttttatactctctaaaaacacctctataatacatactcagacctaatatagcaataaatttatgaataatagtatttgatttgaataaaatgttaatgactatcccagtcattataaaactaattgcaattatcaaacgctgcttcatttgtacctcaactttctatttcgtagaaaaaaattcagttcctatccaaaagcctataatagctccaccatatcccc Intra-Species Hit: Count: 1 Min: 1 Max: 617 Len: 617 Subject: smit_SMT_804_SMT_805|S-adenosylmethionine synthetase:hypothetical protein predicted by Glimmer/Critica|NEGATIVE:NEGATIVE|[715165,715781]|617 HSP 1 e-value: 0.0 bit: 1181.0 Len: 617 Query Start:1 Query End:617 Subject Strand: POSITIVE Subject Start: 1 Subject End: 617 aaaaatgtccccccttaaaaatagtgttatagagttacaaagtactgataggtattttcattttctaagagttacaacgcgtaagaaataaaaacgatacttttttatttctgaagctagtaaggcgcatagggtgaccgcattatagcggcaaccgtagaatgacgaacgacttttgagccgtcattctttgcgagttgcgactcgaaagaaaaaatagaaatttctaccatcttatcgggactatataactttaccattataccatttttaaactcaatttgcagtctataatgtagtgtttctgtatagatagttcgttctcataaaacaaaaaggatttaatcctgttatatacagaattaaatcaaaatctgaattgtttttaaattttttatactctctaaaaacacctctataatacatactcagacctaatatagcaataaatttatgaataatagtatttgatttgaataaaatgttaatgactatcccagtcattataaaactaattgcaattatcaaacgctgcttcatttgtacctcaactttctatttcgtagnnnnnnnttcagttcctatccaaaagcctataatagctccaccatatcccc aaaaatgtccccccttaaaaatagtgttatagagttacaaagtactgataggtattttcattttctaagagttacaacgcgtaagaaataaaaacgatacttttttatttctgaagctagtaaggcgcatagggtgaccgcattatagcggcaaccgtagaatgacgaacgacttttgagccgtcattctttgcgagttgcgactcgaaagaaaaaatagaaatttctaccatcttatcgggactatataactttaccattataccatttttaaactcaatttgcagtctataatgtagtgtttctgtatagatagttcgttctcataaaacaaaaaggatttaatcctgttatatacagaattaaatcaaaatctgaattgtttttaaattttttatactctctaaaaacacctctataatacatactcagacctaatatagcaataaatttatgaataatagtatttgatttgaataaaatgttaatgactatcccagtcattataaaactaattgcaattatcaaacgctgcttcatttgtacctcaactttctatttcgtagaaaaaaattcagttcctatccaaaagcctataatagctccaccatatcccc Inter-species Hit: Count: 3 Min: 1 Max: 338 Len: 338 Subject: NC_008533_SPD_0663_SPD_0664|ATP-dependent RNA helicase, DEAD/DEAH box family protein:S-adenosylmethionine synthetase|POSITIVE:POSITIVE|[683436,683649]|214 HSP 1 e-value: 6.0E-40 bit: 167.0 Len: 112 Query Start:227 Query End:338 Subject Strand: NEGATIVE Subject Start: 29 Subject End: 140 ..................................................................................................................................................................................................................................ctaccatcttatcgggactatataactttaccattataccatttttaaactcaatttgcagtctataatgtagtgtttctgtatagatagttcgttctcataaaacaaaaag....................................................................................................................................................................................................................................................................................... ..................................................................................................................................................................................................................................ctaccatcttatcgggactatataactttatcattataccatttttaatcgcaatttgcagtctttaatgtagtgtttcggtttagatagttcgttctcataaagcaaaaag....................................................................................................................................................................................................................................................................................... HSP 2 e-value: 3.0E-14 bit: 81.8 Len: 52 Query Start:1 Query End:52 Subject Strand: NEGATIVE Subject Start: 162 Subject End: 214 aaaaatgtccccccttaaaaa-tagtgttatagagttacaaagtactgatagg..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... aaaaatgtccccccttaaaaaatattgttatagagttacaaagtactgatagg..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Subject: NC_003098_spr0670_spr0671|ATP-dependent RNA helicase:S-adenosylmethionine synthetase|POSITIVE:POSITIVE|[675905,676118]|214 HSP 1 e-value: 6.0E-40 bit: 167.0 Len: 112 Query Start:227 Query End:338 Subject Strand: NEGATIVE Subject Start: 29 Subject End: 140 ..................................................................................................................................................................................................................................ctaccatcttatcgggactatataactttaccattataccatttttaaactcaatttgcagtctataatgtagtgtttctgtatagatagttcgttctcataaaacaaaaag....................................................................................................................................................................................................................................................................................... ..................................................................................................................................................................................................................................ctaccatcttatcgggactatataactttatcattataccatttttaatcgcaatttgcagtctttaatgtagtgtttcggtttagatagttcgttctcataaagcaaaaag....................................................................................................................................................................................................................................................................................... HSP 2 e-value: 3.0E-14 bit: 81.8 Len: 52 Query Start:1 Query End:52 Subject Strand: NEGATIVE Subject Start: 162 Subject End: 214 aaaaatgtccccccttaaaaa-tagtgttatagagttacaaagtactgatagg..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... aaaaatgtccccccttaaaaaatattgttatagagttacaaagtactgatagg..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Subject: NC_003028_SP_0761_SP_0762|ATP-dependent RNA helicase, DEAD/DEAH box family:S-adenosylmethionine synthetase|POSITIVE:POSITIVE|[721244,721457]|214 HSP 1 e-value: 1.0E-37 bit: 159.0 Len: 104 Query Start:227 Query End:330 Subject Strand: NEGATIVE Subject Start: 37 Subject End: 140 ..................................................................................................................................................................................................................................ctaccatcttatcgggactatataactttaccattataccatttttaaactcaatttgcagtctataatgtagtgtttctgtatagatagttcgttctcataaa............................................................................................................................................................................................................................................................................................... ..................................................................................................................................................................................................................................ctaccatcttatcgggactatataactttatcattataccatttttaatcgcaatttgcagtctttaatgtagtgtttcggtttagatagttcgttctcataaa............................................................................................................................................................................................................................................................................................... HSP 2 e-value: 3.0E-14 bit: 81.8 Len: 52 Query Start:1 Query End:52 Subject Strand: NEGATIVE Subject Start: 162 Subject End: 214 aaaaatgtccccccttaaaaa-tagtgttatagagttacaaagtactgatagg..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... aaaaatgtccccccttaaaaaatattgttatagagttacaaagtactgatagg..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAAAAUGUCCCCCCUUAAAAAUAGUGUUAUAGAGUUACAAAGUACUGAUAGGUAUUUUCAUUUUCUAAGAGUUACAACGCGUAAGAAAUAAAAACGAUACUUUUUUAUUUCUGAAGCUAGUAAGGCGCAUAGGGUGACCGCAUUAUAGCGGCAACCGUAGAAUGACGAACGACUUUUGAGCCGUCAUUCUUUGCGAGUUGCGACUCGAAAGAAAAAAUAGAAAUUUCUACCAUCUUAUCGGGACUAUAUAACUUUACCAUUAUACCAUUUUUAAACUCAAUUUGCAGUCUAUAAUGUAGUGUUUCUGUAUAGAUAGUUCGUUCUCAUAAAACAAAAAG .....(((......((((((((.((((..((((((((.(.(((.((((((((........((((((..((((..((((((...(((((((((((.......)))))))))))...))........((((...(((..((((......))))..)))..(((((((((..............))))))))).)))).))))..))))...))))))..((((....))))....)))))))).))).).)))))))).....)))).))))))))....(((..((.(((((((...(((.....)))))))))).))..)))........)))..... (-81.34) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CUUUUUGUUUUAUGAGAACGAACUAUCUAUACAGAAACACUACAUUAUAGACUGCAAAUUGAGUUUAAAAAUGGUAUAAUGGUAAAGUUAUAUAGUCCCGAUAAGAUGGUAGAAAUUUCUAUUUUUUCUUUCGAGUCGCAACUCGCAAAGAAUGACGGCUCAAAAGUCGUUCGUCAUUCUACGGUUGCCGCUAUAAUGCGGUCACCCUAUGCGCCUUACUAGCUUCAGAAAUAAAAAAGUAUCGUUUUUAUUUCUUACGCGUUGUAACUCUUAGAAAAUGAAAAUACCUAUCAGUACUUUGUAACUCUAUAACACUAUUUUUAAGGGGGGACAUUUUU (..((((((((((((..........(((....))).........)))))))..)))))..)..((((((((((((.(.((((...(((((((.(((.(.((((....((((....((((....((((((...((((..((((.((((.(((((((((((.........).))))))))))..(((.(((((......))))).)))...))))........((...(((((((((((.......)))))))))))...))))))..))))..)))))).)))).)))))))).).))).))))))))))).).)))))))))))).(....)...... (-83.91) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 874 140 91 231 182 smit:SMT_804|5end_S-adenosylmethionine_synthetase_715074..715304_NEGATIVE 357 180 131 224 154 smit:SMT_414|5end_K03665_GTP-binding_protein_HflX_357554..357784_POSITIVE 350 157 113 53 13 smit:SMT_1606|5end_glutamate_racemase_1595857..1596087_POSITIVE 324 157 111 173 120 smit:SMT_2067|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1973432..1973662_POSITIVE 322 210 161 75 23 smit:SMT_1896|5end_lysine_decarboxylase_1848735..1848965_NEGATIVE 308 141 111 230 198 smit:SMT_557|5end_K04488_nitrogen_fixation_protein_NifU_and_related_proteins_474757..474987_POSITIVE 306 158 111 57 12 smit:SMT_1470|5end_K06188_aquaporin_Z_1452560..1452790_POSITIVE 306 160 111 223 167 smit:SMT_1189|5end_phosphoribosylaminoimidazole_carboxylase_ATPase_subunit_1147754..1147984_NEGATIVE 302 149 107 70 36 smit:SMT_1593|5end_ABC-type_oligopeptide_transport_system,_periplasmic_component_1581417..1581647_POSITIVE 302 178 131 222 164 smit:SMT_676|5end_DNA_helicase_II_585266..585496_NEGATIVE 290 160 115 65 17 smit:SMT_324|5end_K02029_polar_amino_acid_transport_system_permease_protein_262070..262300_NEGATIVE 287 159 111 83 40 smit:SMT_2039|5end_malolactic_enzyme_1950427..1950657_NEGATIVE 286 164 123 62 8 smit:SMT_1884|5end_hypothetical_protein_1838964..1839194_POSITIVE 286 164 123 62 8 smit:SMT_1875|5end_hypothetical_protein_1832965..1833195_POSITIVE 286 203 162 69 29 smit:SMT_302|5end_K07444_putative_N6-adenine-specific_DNA_methylase_244808..245038_POSITIVE 283 140 94 209 152 smit:SMT_1718|5end_Holliday_junction_DNA_helicase_RuvA_1704877..1705107_NEGATIVE 283 207 164 194 137 smit:SMT_1078|5end_folylpolyglutamate_synthase_1016713..1016943_NEGATIVE 280 185 143 202 156 smit:SMT_1053|5end_ABC_transporter_permease_protein_993671..993901_NEGATIVE 277 152 115 205 177 smit:SMT_1668|5end_K+_transport_system,_NAD-binding_component_1658288..1658518_NEGATIVE 277 150 115 146 108 smit:SMT_1013|5end_galactose-1-phosphate_uridylyltransferase_950626..950856_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 317 159 111 59 11 smit:SMT_640|3end_PTS_system,_lactose-specific_IIBC_component_545099..545249_NEGATIVE 308 210 166 44 3 smit:SMT_1897|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1848766..1848916_NEGATIVE 306 158 111 49 4 smit:SMT_1469|3end_GTP_pyrophosphokinase_1452632..1452782_POSITIVE 305 160 116 61 13 smit:SMT_1867|3end_peptide_chain_release_factor_1_1827910..1828060_NEGATIVE 294 196 160 140 102 smit:SMT_1360|3end_lactoylglutathione_lyase_1338252..1338402_NEGATIVE 287 159 111 47 4 smit:SMT_2040|3end_transcription-repair_coupling_factor_1950463..1950613_NEGATIVE 277 150 115 127 89 smit:SMT_1014|3end_galactokinase_950645..950795_NEGATIVE 272 325 284 136 94 smit:SMT_1992|3end_carboxynorspermidine_decarboxylase_1918557..1918707_NEGATIVE 269 149 115 76 39 smit:SMT_2057|3end_cell_division_protein_FtsH_1966897..1967047_NEGATIVE 268 159 111 76 10 smit:SMT_1970|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1903032..1903182_POSITIVE 262 200 152 60 14 smit:SMT_1880|3end_hypothetical_protein_1837434..1837584_POSITIVE 262 182 142 74 31 smit:SMT_1776|3end_K07707_two-component_system,_AgrA_family,_response_regulator_AgrA_1751892..1752042_NEGATIVE 262 200 152 60 14 smit:SMT_74|3end_hypothetical_protein_947..1097_NEGATIVE 260 210 170 53 13 smit:SMT_458|3end_hypothetical_protein_predicted_by_Glimmer/Critica_396729..396879_NEGATIVE 259 198 155 59 8 smit:SMT_2149|3end_UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-_diaminopimelate--D-alanyl-D-alanine_ligase_2024734..2024884_POSITIVE 258 141 105 113 74 smit:SMT_1898|3end_aminotransferase_(class_V),_putative_1848877..1849027_NEGATIVE 257 160 118 140 92 smit:SMT_807|3end_hypothetical_protein_predicted_by_Glimmer/Critica_716708..716858_NEGATIVE 256 200 159 79 35 smit:SMT_1303|3end_tRNA-dihydrouridine_synthase_1264676..1264826_NEGATIVE 255 151 111 71 36 smit:SMT_1559|3end_6-phosphofructokinase_1544614..1544764_NEGATIVE 255 153 124 66 36 smit:SMT_1344|3end_oligoendopeptidase_F_1320996..1321146_NEGATIVE