Origin IGS: ttgatagattctccttaaaattttttagacaatccttattctatcaatttttatatcttttagcaacaggttttctaaaagaatatatgggaaagcggttttatttctcttattgaaacaaaaggattaaagaggtggatttccttaatccgagagttc .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 gaactctcggattaaggaaatccacctctttaatccttttgtttcaataagagaaataaaaccgctttcccatatattcttttagaaaacctgttgctaaaagatataaaaattgatagaataaggattgtctaaaaaattttaaggagaatctatcaa Mask Tandem Repeat Region ================================================ ttgatagattctccttaaaattttttagacaatccttattctatcaatttttatatcttttagcaacaggttttctaaaagaatatatgggaaagcggttttatttctcttattgaaacaaaaggattaaagaggtggatttccttaatccgagagttc Find is-nt database================================================ Query_seq: SMT_787:SMT_788|SMT_787:SMT_788:K02440 glycerol uptake facilitator protein:hypothetical protein:<-<-:699084..699242 159 ttgatagattctccttaaaattttttagacaatccttattctatcaatttttatatcttttagcaacaggttttctaaaagaatatatgggaaagcggttttatttctcttattgaaacaaaaggattaaagaggtggatttccttaatccgagagttc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_787:SMT_788|SMT_787:SMT_788:K02440 glycerol uptake facilitator protein:hypothetical protein:<-<-:699084..699242 159 ttgatagattctccttaaaattttttagacaatccttattctatcaatttttatatcttttagcaacaggttttctaaaagaatatatgggaaagcggttttatttctcttattgaaacaaaaggattaaagaggtggatttccttaatccgagagttc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_787:SMT_788|SMT_787:SMT_788:K02440 glycerol uptake facilitator protein:hypothetical protein:<-<-:699084..699242 159 ttgatagattctccttaaaattttttagacaatccttattctatcaatttttatatcttttagcaacaggttttctaaaagaatatatgggaaagcggttttatttctcttattgaaacaaaaggattaaagaggtggatttccttaatccgagagttc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ttgatagattctccttaaaattttttagacaatccttattctatcaatttttatatcttttagcaacaggttttctaaaagaatatatgggaaagcggttttatttctcttattgaaacaaaaggattaaagaggtggatttccttaatccgagagttc Predict ORF larger than 30AA ================================================ Protein_Len: 32 Strand: + Start: 32 End: 127 ............................... M L I L S I F I S F S N R F S K R I Y G K A V L F L L L K Q K D ................................ ttgatagattctccttaaaattttttagacaatccttattctatcaatttttatatcttttagcaacaggttttctaaaagaatatatgggaaagcggttttatttctcttattgaaacaaaaggattaaagaggtggatttccttaatccgagagttc Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: - Score: 84 Start: 38 End: 67 .....................................GTTGCTAAAAGATATAAAAATTGATAGAAT............................................................................................ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ttgatagattctccttaaaattttttagacaatccttattctatcaatttttatatcttttagcaacaggttttctaaaagaatatatgggaaagcggttttatttctcttattgaaacaaaaggattaaagaggtggatttccttaatccgagagttc Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: SMT_787:SMT_788|SMT_787:SMT_788:K02440 glycerol uptake facilitator protein:hypothetical protein:<-<-:699084..699242 159 ttgatagattctccttaaaattttttagacaatccttattctatcaatttttatatcttttagcaacaggttttctaaaagaatatatgggaaagcggttttatttctcttattgaaacaaaaggattaaagaggtggatttccttaatccgagagttc Intra-Species Hit: Count: 1 Min: 1 Max: 159 Len: 159 Subject: smit_SMT_787_SMT_788|K02440 glycerol uptake facilitator protein:hypothetical protein|NEGATIVE:NEGATIVE|[699084,699242]|159 HSP 1 e-value: 2.0E-85 bit: 315.0 Len: 159 Query Start:1 Query End:159 Subject Strand: POSITIVE Subject Start: 1 Subject End: 159 ttgatagattctccttaaaattttttagacaatccttattctatcaatttttatatcttttagcaacaggttttctaaaagaatatatgggaaagcggttttatttctcttattgaaacaaaaggattaaagaggtggatttccttaatccgagagttc ttgatagattctccttaaaattttttagacaatccttattctatcaatttttatatcttttagcaacaggttttctaaaagaatatatgggaaagcggttttatttctcttattgaaacaaaaggattaaagaggtggatttccttaatccgagagttc Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUGAUAGAUUCUCCUUAAAAUUUUUUAGACAAUCCUUAUUCUAUCAAUUUUUAUAUCUUUUAGCAACAGGUUUUCUAAAAGAAUAUAUGGGAAAGCGGUUUUAUUUCUCUUAUUGAAACAAAAGGAUUAAAGAGGUGGAUUUCCUUAAUCCGAGAGUUC ......(((((((.................(((((((......(((((...((((((((((((.((.....)).))))))).))))).((((((.........))))))..))))).....)))))))...((((.......)))).....))))))). (-27.80) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GAACUCUCGGAUUAAGGAAAUCCACCUCUUUAAUCCUUUUGUUUCAAUAAGAGAAAUAAAACCGCUUUCCCAUAUAUUCUUUUAGAAAACCUGUUGCUAAAAGAUAUAAAAAUUGAUAGAAUAAGGAUUGUCUAAAAAAUUUUAAGGAGAAUCUAUCAA ........((((((((((........)))))))))).((((((((.......)))))))).....(((((.....(((.(((((((...(((....(((...(((......)))..)))....)))....)))))))))).....)))))......... (-29.40) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 786 110 61 201 152 smit:SMT_787|5end_K02440_glycerol_uptake_facilitator_protein_698993..699223_NEGATIVE 325 69 22 213 165 smit:SMT_969|5end_30S_ribosomal_protein_S15_914922..915152_NEGATIVE 247 112 89 87 63 smit:SMT_110|5end_alpha-L-fucosidase_57445..57675_POSITIVE 242 139 93 81 43 smit:SMT_580|5end_K04094_glucose_inhibited_division_protein_Gid_497421..497651_POSITIVE 241 135 102 184 148 smit:SMT_1124|5end_pyrrolidone-carboxylate_peptidase_1060869..1061099_NEGATIVE 237 110 63 63 18 smit:SMT_1112|5end_K02248_competence_protein_ComGF_1051848..1052078_NEGATIVE 234 111 85 208 183 smit:SMT_964|5end_cadmium_resistance_transporter,_putative_910511..910741_NEGATIVE 220 49 6 113 59 smit:SMT_1376|5end_tetrapyrrole_(corrin/porphyrin)_methylase_family_protein_1355553..1355783_NEGATIVE 215 99 53 134 79 smit:SMT_749|5end_shikimate_kinase_653971..654201_NEGATIVE 206 73 34 223 181 smit:SMT_269|5end_phospho-N-acetylmuramoyl-pentapeptide-transferas_e_207725..207955_POSITIVE 206 134 92 53 3 smit:SMT_229|5end_mechanosensitive_ion_channel_165623..165853_NEGATIVE 204 147 107 227 185 smit:SMT_1083|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1020829..1021059_POSITIVE 203 46 1 108 66 smit:SMT_1597|5end_K02032_peptide/nickel_transport_system_ATP-binding_protein_1586981..1587211_POSITIVE 202 80 32 149 97 smit:SMT_576|5end_transcriptional_regulator,_LysR_family_495157..495387_POSITIVE 201 68 23 112 71 smit:SMT_2075|5end_phosphoribosylamine--glycine_ligase_1978485..1978715_NEGATIVE 201 58 12 173 121 smit:SMT_2022|5end_hypothetical_protein_1938257..1938487_POSITIVE 201 140 94 192 147 smit:SMT_736|5end_hypothetical_protein_642270..642500_NEGATIVE 198 139 94 50 2 smit:SMT_1528|5end_K03310_alanine_or_glycine:cation_symporter,_AGCS_family_1512667..1512897_NEGATIVE 198 82 41 135 85 smit:SMT_1438|5end_hypothetical_protein_1422877..1423107_NEGATIVE 198 99 57 54 16 smit:SMT_313|5end_3R-hydroxymyristoyl_ACP_dehydrase_253843..254073_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 247 112 89 85 61 smit:SMT_109|3end_beta-galactosidase_57523..57673_POSITIVE 241 135 102 78 42 smit:SMT_1125|3end_K08987_putative_membrane_protein_1060975..1061125_NEGATIVE 237 110 63 100 55 smit:SMT_1113|3end_hypothetical_protein_1051811..1051961_NEGATIVE 220 49 6 110 56 smit:SMT_1377|3end_hypothetical_protein_1355556..1355706_NEGATIVE 215 99 53 141 86 smit:SMT_750|3end_3-phosphoshikimate_1-carboxyvinyltransferase_653964..654114_NEGATIVE 210 110 66 43 7 smit:SMT_1074|3end_catabolite_control_protein_A_1012275..1012425_NEGATIVE 204 126 83 70 20 smit:SMT_1806|3end_aminoacylase/N-acyl-L-amino_acid_amidohydrolase/hippurate_hydrolase_1781644..1781794_POSITIVE 203 46 1 97 55 smit:SMT_1596|3end_oligopeptide_transport_ATP-binding_protein_OppD_1587050..1587200_POSITIVE 202 110 65 97 47 smit:SMT_516|3end_hypothetical_protein_442527..442677_NEGATIVE 201 68 23 115 74 smit:SMT_2076|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1978482..1978632_NEGATIVE 201 58 12 130 78 smit:SMT_2021|3end_K06201_copper_homeostasis_protein_1938294..1938444_POSITIVE 198 99 57 57 19 smit:SMT_312|3end_acetyl-CoA_carboxylase,_biotin_carboxyl_carrier_protein_253926..254076_POSITIVE 197 107 62 150 113 smit:SMT_212|3end_Holliday_junction_DNA_helicase_RuvB_145299..145449_POSITIVE 194 96 63 65 37 smit:SMT_1866|3end_extramembranal_protein_1827939..1828089_POSITIVE 191 135 94 60 5 smit:SMT_653|3end_positive_transcriptional_regulator_MutR_family_563292..563442_NEGATIVE 189 92 54 131 92 smit:SMT_1623|3end_K03498_trk_system_potassium_uptake_protein_TrkH_1611115..1611265_POSITIVE 189 144 105 61 27 smit:SMT_1062|3end_hypothetical_protein_1000648..1000798_POSITIVE 187 74 39 131 98 smit:SMT_846|3end_hypothetical_protein_791855..792005_NEGATIVE 186 77 33 107 64 smit:SMT_341|3end_ThiJ/PfpI_family_protein_281681..281831_POSITIVE 185 117 73 107 62 smit:SMT_941|3end_hypothetical_protein_892863..893013_NEGATIVE