Origin IGS: ctttttcctttctcaaaagaaatatcttttttcaatattctatcacaaataagggcgaattaccaactaaacaaggcgaagtttgactttttctagtttattggggtacaatagagaaaagatagaaatgaagtgaaaaat .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 atttttcacttcatttctatcttttctctattgtaccccaataaactagaaaaagtcaaacttcgccttgtttagttggtaattcgcccttatttgtgatagaatattgaaaaaagatatttcttttgagaaaggaaaaag Mask Tandem Repeat Region ================================================ ctttttcctttctcaaaagaaatatcttttttcaatattctatcacaaataagggcgaattaccaactaaacaaggcgaagtttgactttttctagtttattggggtacaatagagaaaagatagaaatgaagtgaaaaat Find is-nt database================================================ Query_seq: SMT_782:SMT_783|SMT_782:SMT_783:GMP synthase:K03710 GntR family transcriptional regulator:<-->:694610..694750 141 ctttttcctttctcaaaagaaatatcttttttcaatattctatcacaaataagggcgaattaccaactaaacaaggcgaagtttgactttttctagtttattggggtacaatagagaaaagatagaaatgaagtgaaaaat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_782:SMT_783|SMT_782:SMT_783:GMP synthase:K03710 GntR family transcriptional regulator:<-->:694610..694750 141 ctttttcctttctcaaaagaaatatcttttttcaatattctatcacaaataagggcgaattaccaactaaacaaggcgaagtttgactttttctagtttattggggtacaatagagaaaagatagaaatgaagtgaaaaat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_782:SMT_783|SMT_782:SMT_783:GMP synthase:K03710 GntR family transcriptional regulator:<-->:694610..694750 141 ctttttcctttctcaaaagaaatatcttttttcaatattctatcacaaataagggcgaattaccaactaaacaaggcgaagtttgactttttctagtttattggggtacaatagagaaaagatagaaatgaagtgaaaaat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ctttttcctttctcaaaagaaatatcttttttcaatattctatcacaaataagggcgaattaccaactaaacaaggcgaagtttgactttttctagtttattggggtacaatagagaaaagatagaaatgaagtgaaaaat Predict ORF larger than 30AA ================================================ ctttttcctttctcaaaagaaatatcttttttcaatattctatcacaaataagggcgaattaccaactaaacaaggcgaagtttgactttttctagtttattggggtacaatagagaaaagatagaaatgaagtgaaaaat Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ctttttcctttctcaaaagaaatatcttttttcaatattctatcacaaataagggcgaattaccaactaaacaaggcgaagtttgactttttctagtttattggggtacaatagagaaaagatagaaatgaagtgaaaaat Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: SMT_782:SMT_783|SMT_782:SMT_783:GMP synthase:K03710 GntR family transcriptional regulator:<-->:694610..694750 141 ctttttcctttctcaaaagaaatatcttttttcaatattctatcacaaataagggcgaattaccaactaaacaaggcgaagtttgactttttctagtttattggggtacaatagagaaaagatagaaatgaagtgaaaaat Intra-Species Hit: Count: 1 Min: 1 Max: 141 Len: 141 Subject: smit_SMT_782_SMT_783|GMP synthase:K03710 GntR family transcriptional regulator|NEGATIVE:POSITIVE|[694610,694750]|141 HSP 1 e-value: 1.0E-74 bit: 280.0 Len: 141 Query Start:1 Query End:141 Subject Strand: POSITIVE Subject Start: 1 Subject End: 141 ctttttcctttctcaaaagaaatatcttttttcaatattctatcacaaataagggcgaattaccaactaaacaaggcgaagtttgactttttctagtttattggggtacaatagagaaaagatagaaatgaagtgaaaaat ctttttcctttctcaaaagaaatatcttttttcaatattctatcacaaataagggcgaattaccaactaaacaaggcgaagtttgactttttctagtttattggggtacaatagagaaaagatagaaatgaagtgaaaaat Inter-species Hit: Count: 3 Min: 1 Max: 141 Len: 141 Subject: NC_008533_SPD_1274_SPD_1275|GMP synthase, C-terminal domain:transcriptional regulator, GntR family protein|NEGATIVE:POSITIVE|[1301700,1301840]|141 HSP 1 e-value: 7.0E-70 bit: 264.0 Len: 141 Query Start:1 Query End:141 Subject Strand: POSITIVE Subject Start: 1 Subject End: 141 ctttttcctttctcaaaagaaatatcttttttcaatattctatcacaaataagggcgaattaccaactaaacaaggcgaagtttgactttttctagtttattggggtacaatagagaaaagatagaaatgaagtgaaaaat ctttttcctttctcaaaagaaatatcttttctcaatattctatcacaaataagggcgaattaccaactaaacaaggcgaagtttgactttttctagtttattggggtgcaatagagaaaagatagaaatgaagtgaaaaat Subject: NC_003028_SP_1445_SP_1446|bifunctional GMP synthase/glutamine amidotransferase protein:transcriptional regulator, GntR family|NEGATIVE:POSITIVE|[1366297,1366428]|132 HSP 1 e-value: 7.0E-67 bit: 254.0 Len: 132 Query Start:1 Query End:132 Subject Strand: POSITIVE Subject Start: 1 Subject End: 132 ctttttcctttctcaaaagaaatatcttttttcaatattctatcacaaataagggcgaattaccaactaaacaaggcgaagtttgactttttctagtttattggggtacaatagagaaaagatagaaatgaa......... ctttttcctttctcaaaagaaatatcttttctcaatattctatcacaaataagggcgaattaccaactaaacaaggcgaagtttgactttttctagtttattggggtacaatagagaaaagatagaaatgaa......... Subject: NC_003098_spr1300_spr1301|bifunctional GMP synthase/glutamine amidotransferase protein:hypothetical protein|NEGATIVE:POSITIVE|[1294169,1294300]|132 HSP 1 e-value: 2.0E-64 bit: 246.0 Len: 132 Query Start:1 Query End:132 Subject Strand: POSITIVE Subject Start: 1 Subject End: 132 ctttttcctttctcaaaagaaatatcttttttcaatattctatcacaaataagggcgaattaccaactaaacaaggcgaagtttgactttttctagtttattggggtacaatagagaaaagatagaaatgaa......... ctttttcctttctcaaaagaaatatcttttctcaatattctatcacaaataagggcgaattaccaactaaacaaggcgaagtttgactttttctagtttattggggtgcaatagagaaaagatagaaatgaa......... Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CUUUUUCCUUUCUCAAAAGAAAUAUCUUUUUUCAAUAUUCUAUCACAAAUAAGGGCGAAUUACCAACUAAACAAGGCGAAGUUUGACUUUUUCUAGUUUAUUGGGGUACAAUAGAGAAAAGAUAGAAAUGAAGUGAAAAAU .((((((.((((..........(((((((((((...((((..((........))..))))..((((.(((((.(((.((((.....)))).))).))))))))).........))))))))))).....)))).)))))). (-27.76) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUUUUUCACUUCAUUUCUAUCUUUUCUCUAUUGUACCCCAAUAAACUAGAAAAAGUCAAACUUCGCCUUGUUUAGUUGGUAAUUCGCCCUUAUUUGUGAUAGAAUAUUGAAAAAAGAUAUUUCUUUUGAGAAAGGAAAAAG .((((((..(((..(((((((............((((....(((((.((...(((.....)))...)).)))))...))))................))))))).......((((((....))))))..)))..)))))). (-22.89) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 825 90 41 181 132 smit:SMT_782|5end_GMP_synthase_694519..694749_NEGATIVE 283 114 72 142 100 smit:SMT_1155|5end_ABC-type_oligopeptide_transport_system,_periplasmic_component_1099665..1099895_NEGATIVE 269 122 84 135 91 smit:SMT_1359|5end_dihydroorotate_dehydrogenase_electron_transfer_subunit_1338003..1338233_NEGATIVE 260 116 71 208 172 smit:SMT_2030|5end_chromosomal_replication_initiator_protein_DnaA_1945167..1945397_NEGATIVE 260 116 71 148 112 smit:SMT_2002|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1924405..1924635_NEGATIVE 260 116 71 187 151 smit:SMT_1966|5end_DNA_polymerase_III_alpha_subunit_1897934..1898164_POSITIVE 260 116 71 148 112 smit:SMT_1942|5end_zinc_metalloprotease_ZmpB,_truncated_1877882..1878112_POSITIVE 260 116 71 231 195 smit:SMT_1927|5end_cell_division_protein_FtsH_1868779..1869009_NEGATIVE 260 116 71 188 152 smit:SMT_1926|5end_mevalonate_kinase_1867692..1867922_POSITIVE 260 116 71 169 133 smit:SMT_1885|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1840057..1840287_NEGATIVE 260 116 71 216 180 smit:SMT_1672|5end_hypothetical_protein_1660051..1660281_NEGATIVE 259 110 62 90 50 smit:SMT_1955|5end_carbamoyl-phosphate_synthase_small_chain_1889408..1889638_NEGATIVE 259 88 41 174 119 smit:SMT_746|5end_LPS_biosynthesis_protein_651222..651452_NEGATIVE 258 109 71 230 198 smit:SMT_1873|5end_K02006_cobalt_transport_system_ATP-binding_protein_1831936..1832166_POSITIVE 258 119 75 174 134 smit:SMT_206|5end_K07720_two-component_system,_response_regulator_YesN_138237..138467_NEGATIVE 249 85 44 208 163 smit:SMT_1738|5end_peptidyl-prolyl_cis-trans_isomerase_1720497..1720727_POSITIVE 248 102 73 150 119 smit:SMT_882|5end_acetyl_esterase_830441..830671_NEGATIVE 244 118 72 225 167 smit:SMT_1571|5end_CAAX_amino_terminal_protease_family_protein_1563780..1564010_POSITIVE 243 99 51 95 38 smit:SMT_1639|5end_glycerol-3-phosphate_dehydrogenase_(NAD(P)+)_1625819..1626049_NEGATIVE 238 110 67 134 84 smit:SMT_1925|5end_K03697_ATP-dependent_Clp_protease_ATP-binding_subunit_ClpE_1867262..1867492_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 260 116 71 68 32 smit:SMT_1630|3end_hypothetical_protein_1618141..1618291_POSITIVE 248 102 73 83 52 smit:SMT_883|3end_metallo-beta-lactamase_superfamily_protein_830508..830658_NEGATIVE 233 90 53 56 12 smit:SMT_1116|3end_K02244_competence_protein_ComGB_1052798..1052948_NEGATIVE 226 116 73 120 72 smit:SMT_455|3end_cation_transport_ATPase_393993..394143_POSITIVE 222 88 44 53 1 smit:SMT_747|3end_transcriptional_regulator,_LytR_family_651343..651493_NEGATIVE 221 110 63 66 20 smit:SMT_870|3end_hypothetical_protein_817700..817850_NEGATIVE 219 77 38 150 112 smit:SMT_1993|3end_hypothetical_protein_1919207..1919357_NEGATIVE 219 77 38 150 112 smit:SMT_1416|3end_hypothetical_protein_1399570..1399720_NEGATIVE 218 87 49 62 13 smit:SMT_913|3end_putative_sucrose-specific_PTS_permease,_enzyme_II_863923..864073_NEGATIVE 216 87 49 64 17 smit:SMT_1216|3end_hypothetical_protein_1175969..1176119_POSITIVE 212 108 79 109 79 smit:SMT_703|3end_hypothetical_protein_613729..613879_POSITIVE 211 112 71 108 69 smit:SMT_162|3end_hypothetical_protein_107134..107284_POSITIVE 209 58 25 142 107 smit:SMT_2058|3end_hypoxanthine-guanine_phosphoribosyltransferase_1967299..1967449_NEGATIVE 209 87 54 57 17 smit:SMT_124|3end_hypothetical_protein_75590..75740_NEGATIVE 209 58 25 142 107 smit:SMT_100|3end_hypoxanthine-guanine_phosphoribosyltransferase_32093..32243_POSITIVE 208 91 62 64 35 smit:SMT_551|3end_K07170_GAF_domain-containing_protein_469525..469675_POSITIVE 206 57 19 76 41 smit:SMT_1897|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1848766..1848916_NEGATIVE 206 95 56 77 31 smit:SMT_307|3end_K02078_acyl_carrier_protein_249401..249551_POSITIVE 204 98 55 121 57 smit:SMT_555|3end_K09015_Fe-S_cluster_assembly_protein_SufD_473613..473763_POSITIVE 202 87 51 62 4 smit:SMT_2109|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1999061..1999211_NEGATIVE