Origin IGS: caacacctccaaaaatcaaaagacaagctcatatcacgaagggcgaaaaaccgcggtaccaccttcattcaatgaacttgtcattctcttattcttatgcaattgtatgattgagtagcatgactttctagcttagatggctcgcagcaccgccatttctctggactaagataattgaaaatcaattctcaactttcttattataacgtttttttaagcttgcgtcaactggaaatcatcttaactaatagtagtgaggtaggagggaagaccttttaaaaatttaactactagtttcgaaatgtaagtagtaatatttaatttatttctaaaatctttctatctagcatagccctagaaggctatataaaaccctccagcgctgtttagtatgtcccccctacactgataagattccactcagagtagcttatcttgaacaattttggggattttgtgtcttcgtcatatactacgagatagtgcccttcatcattccaaaacttgcccaatacctcggagtctcttaaatccattttctggtttaagtagtcgctagtatactcaatttttggtgcatcttttaccccagcagtagaaaaactgctaccaataaagataaaagcataaaatagcaccacagcaatccccaagaatttaaagattttatagtttgtattattgttttccatgtttgtatattccttttctttctattttgtgtttccttaaatcccaagagggaattttacccccttatccaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ttggataagggggtaaaattccctcttgggatttaaggaaacacaaaatagaaagaaaaggaatatacaaacatggaaaacaataatacaaactataaaatctttaaattcttggggattgctgtggtgctattttatgcttttatctttattggtagcagtttttctactgctggggtaaaagatgcaccaaaaattgagtatactagcgactacttaaaccagaaaatggatttaagagactccgaggtattgggcaagttttggaatgatgaagggcactatctcgtagtatatgacgaagacacaaaatccccaaaattgttcaagataagctactctgagtggaatcttatcagtgtaggggggacatactaaacagcgctggagggttttatatagccttctagggctatgctagatagaaagattttagaaataaattaaatattactacttacatttcgaaactagtagttaaatttttaaaaggtcttccctcctacctcactactattagttaagatgatttccagttgacgcaagcttaaaaaaacgttataataagaaagttgagaattgattttcaattatcttagtccagagaaatggcggtgctgcgagccatctaagctagaaagtcatgctactcaatcatacaattgcataagaataagagaatgacaagttcattgaatgaaggtggtaccgcggtttttcgcccttcgtgatatgagcttgtcttttgatttttggaggtgttg Mask Tandem Repeat Region ================================================ caacacctccaaaaatcaaaagacaagctcatatcacgaagggcgaaaaaccgcggtaccaccttcattcaatgaacttgtcattctcttattcttatgcaattgtatgattgagtagcatgactttctagcttagatggctcgcagcaccgccatttctctggactaagataattgaaaatcaattctcaactttcttattataacgtttttttaagcttgcgtcaactggaaatcatcttaactaatagtagtgaggtaggagggaagaccttttaaaaatttaactactagtttcgaaatgtaagtagtaatatttaatttatttctaaaatctttctatctagcatagccctagaaggctatataaaaccctccagcgctgtttagtatgtcccccctacactgataagattccactcagagtagcttatcttgaacaattttggggattttgtgtcttcgtcatatactacgagatagtgcccttcatcattccaaaacttgcccaatacctcggagtctcttaaatccattttctggtttaagtagtcgctagtatactcaatttttggtgcatcttttaccccagcagtagaaaaactgctaccaataaagataaaagcataaaatagcaccacagcaatccccaagaatttaaagattttatagtttgtattattgttttccatgtttgtatattccttttctttctattttgtgtttccttaaatcccaagagggaattttacccccttatccaa Find is-nt database================================================ Query_seq: SMT_765:SMT_766|SMT_765:SMT_766:hypothetical protein:hypothetical protein:<-<-:672711..673474 764 caacacctccaaaaatcaaaagacaagctcatatcacgaagggcgaaaaaccgcggtaccaccttcattcaatgaacttgtcattctcttattcttatgcaattgtatgattgagtagcatgactttctagcttagatggctcgcagcaccgccatttctctggactaagataattgaaaatcaattctcaactttcttattataacgtttttttaagcttgcgtcaactggaaatcatcttaactaatagtagtgaggtaggagggaagaccttttaaaaatttaactactagtttcgaaatgtaagtagtaatatttaatttatttctaaaatctttctatctagcatagccctagaaggctatataaaaccctccagcgctgtttagtatgtcccccctacactgataagattccactcagagtagcttatcttgaacaattttggggattttgtgtcttcgtcatatactacgagatagtgcccttcatcattccaaaacttgcccaatacctcggagtctcttaaatccattttctggtttaagtagtcgctagtatactcaatttttggtgcatcttttaccccagcagtagaaaaactgctaccaataaagataaaagcataaaatagcaccacagcaatccccaagaatttaaagattttatagtttgtattattgttttccatgtttgtatattccttttctttctattttgtgtttccttaaatcccaagagggaattttacccccttatccaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_765:SMT_766|SMT_765:SMT_766:hypothetical protein:hypothetical protein:<-<-:672711..673474 764 caacacctccaaaaatcaaaagacaagctcatatcacgaagggcgaaaaaccgcggtaccaccttcattcaatgaacttgtcattctcttattcttatgcaattgtatgattgagtagcatgactttctagcttagatggctcgcagcaccgccatttctctggactaagataattgaaaatcaattctcaactttcttattataacgtttttttaagcttgcgtcaactggaaatcatcttaactaatagtagtgaggtaggagggaagaccttttaaaaatttaactactagtttcgaaatgtaagtagtaatatttaatttatttctaaaatctttctatctagcatagccctagaaggctatataaaaccctccagcgctgtttagtatgtcccccctacactgataagattccactcagagtagcttatcttgaacaattttggggattttgtgtcttcgtcatatactacgagatagtgcccttcatcattccaaaacttgcccaatacctcggagtctcttaaatccattttctggtttaagtagtcgctagtatactcaatttttggtgcatcttttaccccagcagtagaaaaactgctaccaataaagataaaagcataaaatagcaccacagcaatccccaagaatttaaagattttatagtttgtattattgttttccatgtttgtatattccttttctttctattttgtgtttccttaaatcccaagagggaattttacccccttatccaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_765:SMT_766|SMT_765:SMT_766:hypothetical protein:hypothetical protein:<-<-:672711..673474 764 caacacctccaaaaatcaaaagacaagctcatatcacgaagggcgaaaaaccgcggtaccaccttcattcaatgaacttgtcattctcttattcttatgcaattgtatgattgagtagcatgactttctagcttagatggctcgcagcaccgccatttctctggactaagataattgaaaatcaattctcaactttcttattataacgtttttttaagcttgcgtcaactggaaatcatcttaactaatagtagtgaggtaggagggaagaccttttaaaaatttaactactagtttcgaaatgtaagtagtaatatttaatttatttctaaaatctttctatctagcatagccctagaaggctatataaaaccctccagcgctgtttagtatgtcccccctacactgataagattccactcagagtagcttatcttgaacaattttggggattttgtgtcttcgtcatatactacgagatagtgcccttcatcattccaaaacttgcccaatacctcggagtctcttaaatccattttctggtttaagtagtcgctagtatactcaatttttggtgcatcttttaccccagcagtagaaaaactgctaccaataaagataaaagcataaaatagcaccacagcaatccccaagaatttaaagattttatagtttgtattattgttttccatgtttgtatattccttttctttctattttgtgtttccttaaatcccaagagggaattttacccccttatccaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 caacacctccaaaaatcaaaagacaagctcatatcacgaagggcgaaaaaccgcggtaccaccttcattcaatgaacttgtcattctcttattcttatgcaattgtatgattgagtagcatgactttctagcttagatggctcgcagcaccgccatttctctggactaagataattgaaaatcaattctcaactttcttattataacgtttttttaagcttgcgtcaactggaaatcatcttaactaatagtagtgaggtaggagggaagaccttttaaaaatttaactactagtttcgaaatgtaagtagtaatatttaatttatttctaaaatctttctatctagcatagccctagaaggctatataaaaccctccagcgctgtttagtatgtcccccctacactgataagattccactcagagtagcttatcttgaacaattttggggattttgtgtcttcgtcatatactacgagatagtgcccttcatcattccaaaacttgcccaatacctcggagtctcttaaatccattttctggtttaagtagtcgctagtatactcaatttttggtgcatcttttaccccagcagtagaaaaactgctaccaataaagataaaagcataaaatagcaccacagcaatccccaagaatttaaagattttatagtttgtattattgttttccatgtttgtatattccttttctttctattttgtgtttccttaaatcccaagagggaattttacccccttatccaa Predict ORF larger than 30AA ================================================ Protein_Len: 31 Strand: + Start: 15 End: 107 .............. M K R Q A H I T K G E K P R Y H L H S M N L S F S Y S Y A I V ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 34 Strand: + Start: 175 End: 276 .............................................................................................................................................................................. M K I N S Q L S Y Y N V F L S L R Q L E I I L T N S S E V G G K T F ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ Protein_Len: 38 Strand: + Start: 414 End: 527 ............................................................................................................................................................................................................................................................................................................................................................................................................................. M P L R V A Y L E Q F W G F C V F V I Y Y E I V P F I I P K L A Q Y L G V S ............................................................................................................................................................................................................................................. Protein_Len: 38 Strand: + Start: 443 End: 556 .......................................................................................................................................................................................................................................................................................................................................................................................................................................................... M L G I L C L R H I L R D S A L H H S K T C P I P R S L L N P F S G L S S R ................................................................................................................................................................................................................ Protein_Len: 30 Strand: + Start: 674 End: 763 ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. M Y Y C F P C L Y I P F L S I L C F L K S Q E G I L P P Y P . Protein_Len: 32 Strand: + Start: 669 End: 764 ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ M V C I I V F H V C I F L F F L F C V S L N P K R E F Y P L I Q Protein_Len: 51 Strand: - Start: 587 End: 739 .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... G L L L F V A V L L S L L M F Y C W L L G W S N L S K I T Q I I T K W T Q I N R K K R N Q T E K F G M ......................... Protein_Len: 60 Strand: - Start: 390 End: 692 ..................................................................................................................................................................................................................................................................................................................................................................................................... V E S D R L D M K Q N L Y D S T Y E I K P A D K V G A T S F S S G I F I F A Y F L V V A I G L F K F I K Y N T N N N E M ........................................................................ Protein_Len: 34 Strand: - Start: 84 End: 185 ................................................................................... E R I R I C N Y S Q T A H S E L K S P E C C R W K E P S L Y N F I M ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... caacacctccaaaaatcaaaagacaagctcatatcacgaagggcgaaaaaccgcggtaccaccttcattcaatgaacttgtcattctcttattcttatgcaattgtatgattgagtagcatgactttctagcttagatggctcgcagcaccgccatttctctggactaagataattgaaaatcaattctcaactttcttattataacgtttttttaagcttgcgtcaactggaaatcatcttaactaatagtagtgaggtaggagggaagaccttttaaaaatttaactactagtttcgaaatgtaagtagtaatatttaatttatttctaaaatctttctatctagcatagccctagaaggctatataaaaccctccagcgctgtttagtatgtcccccctacactgataagattccactcagagtagcttatcttgaacaattttggggattttgtgtcttcgtcatatactacgagatagtgcccttcatcattccaaaacttgcccaatacctcggagtctcttaaatccattttctggtttaagtagtcgctagtatactcaatttttggtgcatcttttaccccagcagtagaaaaactgctaccaataaagataaaagcataaaatagcaccacagcaatccccaagaatttaaagattttatagtttgtattattgttttccatgtttgtatattccttttctttctattttgtgtttccttaaatcccaagagggaattttacccccttatccaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: - Score: 91 Start: 200 End: 229 .......................................................................................................................................................................................................GTTGACGCAAGCTTAAAAAAACGTTATAAT....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... PromScan Matrix: RpoD-17 Strand: + Score: 82 Start: 656 End: 685 ...............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ATTTAAAGATTTTATAGTTTGTATTATTGT............................................................................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ caacacctccaaaaatcaaaagacaagctcatatcacgaagggcgaaaaaccgcggtaccaccttcattcaatgaacttgtcattctcttattcttatgcaattgtatgattgagtagcatgactttctagcttagatggctcgcagcaccgccatttctctggactaagataattgaaaatcaattctcaactttcttattataacgtttttttaagcttgcgtcaactggaaatcatcttaactaatagtagtgaggtaggagggaagaccttttaaaaatttaactactagtttcgaaatgtaagtagtaatatttaatttatttctaaaatctttctatctagcatagccctagaaggctatataaaaccctccagcgctgtttagtatgtcccccctacactgataagattccactcagagtagcttatcttgaacaattttggggattttgtgtcttcgtcatatactacgagatagtgcccttcatcattccaaaacttgcccaatacctcggagtctcttaaatccattttctggtttaagtagtcgctagtatactcaatttttggtgcatcttttaccccagcagtagaaaaactgctaccaataaagataaaagcataaaatagcaccacagcaatccccaagaatttaaagattttatagtttgtattattgttttccatgtttgtatattccttttctttctattttgtgtttccttaaatcccaagagggaattttacccccttatccaa Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: SMT_765:SMT_766|SMT_765:SMT_766:hypothetical protein:hypothetical protein:<-<-:672711..673474 764 caacacctccaaaaatcaaaagacaagctcatatcacgaagggcgaaaaaccgcggtaccaccttcattcaatgaacttgtcattctcttattcttatgcaattgtatgattgagtagcatgactttctagcttagatggctcgcagcaccgccatttctctggactaagataattgaaaatcaattctcaactttcttattataacgtttttttaagcttgcgtcaactggaaatcatcttaactaatagtagtgaggtaggagggaagaccttttaaaaatttaactactagtttcgaaatgtaagtagtaatatttaatttatttctaaaatctttctatctagcatagccctagaaggctatataaaaccctccagcgctgtttagtatgtcccccctacactgataagattccactcagagtagcttatcttgaacaattttggggattttgtgtcttcgtcatatactacgagatagtgcccttcatcattccaaaacttgcccaatacctcggagtctcttaaatccattttctggtttaagtagtcgctagtatactcaatttttggtgcatcttttaccccagcagtagaaaaactgctaccaataaagataaaagcataaaatagcaccacagcaatccccaagaatttaaagattttatagtttgtattattgttttccatgtttgtatattccttttctttctattttgtgtttccttaaatcccaagagggaattttacccccttatccaa Intra-Species Hit: Count: 1 Min: 1 Max: 764 Len: 764 Subject: smit_SMT_765_SMT_766|hypothetical protein:hypothetical protein|NEGATIVE:NEGATIVE|[672711,673474]|764 HSP 1 e-value: 0.0 bit: 1273.0 Len: 663 Query Start:1 Query End:663 Subject Strand: POSITIVE Subject Start: 1 Subject End: 663 caacacctccaaaaatcaaaagacaagctcatatcacgaagggcgaaaaaccgcggtaccaccttcattcaatgaacttgtcattctcttattcttatgcaattgtatgattgagtagcatgactttctagcttagatggctcgcagcaccgccatttctctggactaagataattgaaaatcaattctcaactttcttattataacgnnnnnnnaagcttgcgtcaactggaaatcatcttaactaatagtagtgaggtaggagggaagaccttttaaaaatttaactactagtttcgaaatgtaagtagtaatatttaatttatttctaaaatctttctatctagcatagccctagaaggctatataaaaccctccagcgctgtttagtatgtcccccctacactgataagattccactcagagtagcttatcttgaacaattttggggattttgtgtcttcgtcatatactacgagatagtgcccttcatcattccaaaacttgcccaatacctcggagtctcttaaatccattttctggtttaagtagtcgctagtatactcaatttttggtgcatcttttaccccagcagtagaaaaactgctaccaataaagataaaagcataaaatagcaccacagcaatccccaagaatttaaag..................................................................................................... caacacctccaaaaatcaaaagacaagctcatatcacgaagggcgaaaaaccgcggtaccaccttcattcaatgaacttgtcattctcttattcttatgcaattgtatgattgagtagcatgactttctagcttagatggctcgcagcaccgccatttctctggactaagataattgaaaatcaattctcaactttcttattataacgtttttttaagcttgcgtcaactggaaatcatcttaactaatagtagtgaggtaggagggaagaccttttaaaaatttaactactagtttcgaaatgtaagtagtaatatttaatttatttctaaaatctttctatctagcatagccctagaaggctatataaaaccctccagcgctgtttagtatgtcccccctacactgataagattccactcagagtagcttatcttgaacaattttggggattttgtgtcttcgtcatatactacgagatagtgcccttcatcattccaaaacttgcccaatacctcggagtctcttaaatccattttctggtttaagtagtcgctagtatactcaatttttggtgcatcttttaccccagcagtagaaaaactgctaccaataaagataaaagcataaaatagcaccacagcaatccccaagaatttaaag..................................................................................................... HSP 2 e-value: 8.0E-12 bit: 73.8 Len: 37 Query Start:728 Query End:764 Subject Strand: POSITIVE Subject Start: 728 Subject End: 764 .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................cttaaatcccaagagggaattttacccccttatccaa .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................cttaaatcccaagagggaattttacccccttatccaa Inter-species Hit: Count: 4 Min: 1 Max: 241 Len: 241 Subject: NC_008533_SPD_1217_SPD_1218|hypothetical protein:spermidine/putrescine ABC transporter, spermidine/putrescine-binding protein|NEGATIVE:NEGATIVE|[1249544,1250317]|774 HSP 1 e-value: 1.0E-109 bit: 396.0 Len: 241 Query Start:1 Query End:241 Subject Strand: POSITIVE Subject Start: 1 Subject End: 241 caacacctccaaaaatcaaaagacaagctcatatcacgaagggcgaaaaaccgcggtaccaccttcattcaatgaacttgtcattctcttattcttatgcaattgtatgattgagtagcatgactttctagcttagatggctcgcagcaccgccatttctctggactaagataattgaaaatcaattctcaactttcttattataacgnnnnnnnaagcttgcgtcaactggaaatcatct........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... caacacctccaaaaatcaaaagacaagctcatatcacgaagggcgaaaaaccgcggtaccaccttcattcaatgaacttgtcattctcttgttcttatgcaattgtatgattgagtagcatgacttcctagcttagatggctcgcagcaccgccatttctctggactaagacaagtgaaaatcaattctcaactttcttattataacgtttttttaagcttgcgtcaactggaaatgatct........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Subject: NC_003098_spr1241_spr1242|hypothetical protein:hypothetical protein|NEGATIVE:NEGATIVE|[1242025,1242286]|262 HSP 1 e-value: 1.0E-102 bit: 373.0 Len: 229 Query Start:13 Query End:241 Subject Strand: POSITIVE Subject Start: 1 Subject End: 229 ............aaatcaaaagacaagctcatatcacgaagggcgaaaaaccgcggtaccaccttcattcaatgaacttgtcattctcttattcttatgcaattgtatgattgagtagcatgactttctagcttagatggctcgcagcaccgccatttctctggactaagataattgaaaatcaattctcaactttcttattataacgnnnnnnnaagcttgcgtcaactggaaatcatct........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... ............aaatcaaaagacaagctcatatcacgaagggcgaaaaaccgcggtaccaccttcattcaatgaacttgtcattctcttgttcttatgcaattgtatgattgagtagcatgacttcctagcttagatggctcgcagcaccgccatttctctggactaagacaagtgaaaatcaattctcaactttcttattataacgtttttttaagcttgcgtcaactggaaatgatct........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Subject: NC_003028_SP_1384_SP_1385|hypothetical protein:hypothetical protein|NEGATIVE:NEGATIVE|[1309465,1309726]|262 HSP 1 e-value: 1.0E-102 bit: 373.0 Len: 229 Query Start:13 Query End:241 Subject Strand: POSITIVE Subject Start: 1 Subject End: 229 ............aaatcaaaagacaagctcatatcacgaagggcgaaaaaccgcggtaccaccttcattcaatgaacttgtcattctcttattcttatgcaattgtatgattgagtagcatgactttctagcttagatggctcgcagcaccgccatttctctggactaagataattgaaaatcaattctcaactttcttattataacgnnnnnnnaagcttgcgtcaactggaaatcatct........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... ............aaatcaaaagacaagctcatatcacgaagggcgaaaaaccgcggtaccaccttcattcaatgaacttgtcattctcttgttcttatgcaattgtatgattgagtagcatgacttcctagcttagatggctcgcagcaccgccatttctctggactaagacaagtgaaaatcaattctcaactttcttattataacgtttttttaagcttgcgtcaactggaaatgatct........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Subject: NC_009009_SSA_0753_SSA_0755|Foldase protein prsA precursor, putative:hypothetical protein|POSITIVE:POSITIVE|[736116,736351]|236 HSP 1 e-value: 9.0E-21 bit: 103.0 Len: 52 Query Start:37 Query End:88 Subject Strand: NEGATIVE Subject Start: 143 Subject End: 194 ....................................cgaagggcgaaaaaccgcggtaccaccttcattcaatgaacttgtcattctc.................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... ....................................cgaagggcgaaaaaccgcggtaccaccttcattcaatgaacttgtcattctc.................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... HSP 2 e-value: 8.0E-9 bit: 63.9 Len: 71 Query Start:135 Query End:205 Subject Strand: NEGATIVE Subject Start: 26 Subject End: 96 ......................................................................................................................................agatggctcgcagcaccgccatttctctggactaagataattg-aaaatcaattctcaactttcttattata............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... ......................................................................................................................................agatggctcgcagcaccgccaattctctgaactaaga-acttgaaaaatcaattctcaccagtcttattata............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CAACACCUCCAAAAAUCAAAAGACAAGCUCAUAUCACGAAGGGCGAAAAACCGCGGUACCACCUUCAUUCAAUGAACUUGUCAUUCUCUUAUUCUUAUGCAAUUGUAUGAUUGAGUAGCAUGACUUUCUAGCUUAGAUGGCUCGCAGCACCGCCAUUUCUCUGGACUAAGAUAAUUGAAAAUCAAUUCUCAACUUUCUUAUUAUAACGUUUUUUUAAGCUUGCGUCAACUGGAAAUCAUCU .......((((..........((((((.((((.....((((((((......))).......))))).....)))).))))))......((((((((((((....))))))..))))))..((((..(((((...(((((((..........)))))))..)))))....((.(((((.....))))).)).................((((....))))....))))..))))........ (-48.81) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGAUGAUUUCCAGUUGACGCAAGCUUAAAAAAACGUUAUAAUAAGAAAGUUGAGAAUUGAUUUUCAAUUAUCUUAGUCCAGAGAAAUGGCGGUGCUGCGAGCCAUCUAAGCUAGAAAGUCAUGCUACUCAAUCAUACAAUUGCAUAAGAAUAAGAGAAUGACAAGUUCAUUGAAUGAAGGUGGUACCGCGGUUUUUCGCCCUUCGUGAUAUGAGCUUGUCUUUUGAUUUUUGGAGGUGUUG ..((((((...(((((((.(.((((((..............(((((.(((((((((....))))))))).)))))..........(((((.(.....)..))))).)))))).)...)))).)))....)))))).((((....(((((((....(((.(((((((((((...((((((((((.((....))...))).)))))))...))))))))))).))).))))))).....)))) (-65.20) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 985 70 21 161 112 smit:SMT_765|5end_hypothetical_protein_672620..672850_NEGATIVE 361 88 41 199 143 smit:SMT_370|5end_acetyltransferase,_GNAT_family_304688..304918_POSITIVE 346 71 36 216 177 smit:SMT_1189|5end_phosphoribosylaminoimidazole_carboxylase_ATPase_subunit_1147754..1147984_NEGATIVE 344 76 33 173 125 smit:SMT_1554|5end_isoleucyl-tRNA_synthetase_1537040..1537270_POSITIVE 341 68 25 140 97 smit:SMT_1685|5end_phenylalanyl-tRNA_synthetase_alpha_chain_1668845..1669075_NEGATIVE 334 155 112 56 18 smit:SMT_639|5end_6-phospho-beta-galactosidase_544885..545115_NEGATIVE 324 64 25 230 180 smit:SMT_1339|5end_hypothetical_protein_1314746..1314976_NEGATIVE 323 69 21 224 172 smit:SMT_970|5end_threonyl-tRNA_synthetase_915772..916002_POSITIVE 319 170 122 76 20 smit:SMT_1634|5end_2,3,4,5-tetrahydropyridine-2-carboxylate_N-succinyltransferase,_putative_1621074..1621304_POSITIVE 318 172 131 183 139 smit:SMT_1647|5end_3-isopropylmalate_dehydratase_small_subunit_1633403..1633633_POSITIVE 308 155 118 205 161 smit:SMT_1386|5end_phosphoenolpyruvate-protein_phosphotransferase_1362806..1363036_POSITIVE 307 151 115 206 168 smit:SMT_794|5end_hypothetical_protein_703487..703717_NEGATIVE 301 46 4 229 179 smit:SMT_1696|5end_hypothetical_protein_1678182..1678412_POSITIVE 300 157 116 46 1 smit:SMT_452|5end_phosphomethylpyrimidine_kinase_390568..390798_NEGATIVE 296 152 113 220 169 smit:SMT_1850|5end_K01421_putative_membrane_protein_1815062..1815292_POSITIVE 294 170 121 65 20 smit:SMT_1610|5end_tyrosine_recombinase_1598565..1598795_POSITIVE 291 157 118 67 21 smit:SMT_1445|5end_hypothetical_protein_1428922..1429152_NEGATIVE 291 86 41 65 12 smit:SMT_1359|5end_dihydroorotate_dehydrogenase_electron_transfer_subunit_1338003..1338233_NEGATIVE 291 167 124 183 136 smit:SMT_568|5end_hypothetical_protein_485674..485904_POSITIVE 290 167 130 65 20 smit:SMT_180|5end_50S_ribosomal_protein_L14_119678..119908_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 294 170 121 74 29 smit:SMT_1609|3end_hypothetical_protein_1598654..1598804_POSITIVE 282 160 111 131 68 smit:SMT_1982|3end_conserved_hypothetical_protein_1913412..1913562_POSITIVE 279 159 113 83 40 smit:SMT_1190|3end_phosphoribosylaminoimidazole_carboxylase,_catalytic_subunit_1147741..1147891_NEGATIVE 277 155 119 139 92 smit:SMT_404|3end_hypothetical_protein_334029..334179_POSITIVE 276 139 94 65 21 smit:SMT_2075|3end_phosphoribosylamine--glycine_ligase_1977769..1977919_NEGATIVE 266 160 111 71 18 smit:SMT_826|3end_hypothetical_protein_749591..749741_NEGATIVE 264 155 111 148 104 smit:SMT_858|3end_triosephosphate_isomerase_802525..802675_NEGATIVE 263 160 115 69 30 smit:SMT_1751|3end_indole-3-glycerol_phosphate_synthase_1729865..1730015_POSITIVE 260 168 124 148 102 smit:SMT_1958|3end_tryptophan_synthase,_beta_subunit_1892052..1892202_POSITIVE 260 168 124 79 33 smit:SMT_362|3end_K02519_translation_initiation_factor_IF-2_297950..298100_POSITIVE 260 167 129 39 2 smit:SMT_179|3end_ribosomal_protein_S17_119732..119882_POSITIVE 259 222 183 73 35 smit:SMT_1868|3end_thymidine_kinase_1828215..1828365_NEGATIVE 257 155 115 145 101 smit:SMT_1367|3end_K02237_competence_protein_ComEA_1346872..1347022_NEGATIVE 256 169 121 64 6 smit:SMT_2073|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1977074..1977224_POSITIVE 256 170 121 150 104 smit:SMT_977|3end_conserved_hypothetical_protein,_Rrf2_family_protein_925252..925402_NEGATIVE 254 153 111 138 100 smit:SMT_807|3end_hypothetical_protein_predicted_by_Glimmer/Critica_716708..716858_NEGATIVE 253 165 121 60 10 smit:SMT_1036|3end_alpha-galactosidase_978160..978310_NEGATIVE 251 165 125 120 70 smit:SMT_1649|3end_GMP_reductase_1636068..1636218_POSITIVE 250 87 41 98 59 smit:SMT_1871|3end_modification_methylase,_HemK_family_1829803..1829953_NEGATIVE 250 165 122 150 106 smit:SMT_418|3end_putative_transcriptional_regulator_361232..361382_NEGATIVE