Origin IGS: agccctataaaagatgagaccaaccaatctcatctttttttcgtatcaaatctaagcaattttagaaactttatggcatactagacttatcaatgaaagcgaggtaatctc .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 gagattacctcgctttcattgataagtctagtatgccataaagtttctaaaattgcttagatttgatacgaaaaaaagatgagattggttggtctcatcttttatagggct Mask Tandem Repeat Region ================================================ agccctataaaagatgagaccaaccaatctcatctttttttcgtatcaaatctaagcaattttagaaactttatggcatactagacttatcaatgaaagcgaggtaatctc Find is-nt database================================================ Query_seq: SMT_655:SMT_656|SMT_655:SMT_656:phosphoglucomutase:bacteriocin transport accessory protein, putative:->->:568021..568131 111 agccctataaaagatgagaccaaccaatctcatctttttttcgtatcaaatctaagcaattttagaaactttatggcatactagacttatcaatgaaagcgaggtaatctc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_655:SMT_656|SMT_655:SMT_656:phosphoglucomutase:bacteriocin transport accessory protein, putative:->->:568021..568131 111 agccctataaaagatgagaccaaccaatctcatctttttttcgtatcaaatctaagcaattttagaaactttatggcatactagacttatcaatgaaagcgaggtaatctc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_655:SMT_656|SMT_655:SMT_656:phosphoglucomutase:bacteriocin transport accessory protein, putative:->->:568021..568131 111 agccctataaaagatgagaccaaccaatctcatctttttttcgtatcaaatctaagcaattttagaaactttatggcatactagacttatcaatgaaagcgaggtaatctc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 agccctataaaagatgagaccaaccaatctcatctttttttcgtatcaaatctaagcaattttagaaactttatggcatactagacttatcaatgaaagcgaggtaatctc Predict ORF larger than 30AA ================================================ agccctataaaagatgagaccaaccaatctcatctttttttcgtatcaaatctaagcaattttagaaactttatggcatactagacttatcaatgaaagcgaggtaatctc Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ agccctataaaagatgagaccaaccaatctcatctttttttcgtatcaaatctaagcaattttagaaactttatggcatactagacttatcaatgaaagcgaggtaatctc Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: SMT_655:SMT_656|SMT_655:SMT_656:phosphoglucomutase:bacteriocin transport accessory protein, putative:->->:568021..568131 111 agccctataaaagatgagaccaaccaatctcatctttttttcgtatcaaatctaagcaattttagaaactttatggcatactagacttatcaatgaaagcgaggtaatctc Intra-Species Hit: Count: 1 Min: 1 Max: 111 Len: 111 Subject: smit_SMT_655_SMT_656|phosphoglucomutase:bacteriocin transport accessory protein, putative|POSITIVE:POSITIVE|[568021,568131]|111 HSP 1 e-value: 2.0E-44 bit: 178.0 Len: 111 Query Start:1 Query End:111 Subject Strand: POSITIVE Subject Start: 1 Subject End: 111 agccctataaaagatgagaccaaccaatctcatcnnnnnnncgtatcaaatctaagcaattttagaaactttatggcatactagacttatcaatgaaagcgaggtaatctc agccctataaaagatgagaccaaccaatctcatctttttttcgtatcaaatctaagcaattttagaaactttatggcatactagacttatcaatgaaagcgaggtaatctc Inter-species Hit: Count: 3 Min: 4 Max: 111 Len: 108 Subject: NC_008533_SPD_1326_SPD_1327|phosphoglucomutase/phosphomannomutase family protein:bacterocin transport accessory protein|POSITIVE:POSITIVE|[1342898,1343007]|110 HSP 1 e-value: 3.0E-37 bit: 155.0 Len: 108 Query Start:4 Query End:111 Subject Strand: POSITIVE Subject Start: 4 Subject End: 110 ...cctataaaagatgagaccaaccaatctcatcnnnnnnncgtatcaaatctaagcaattttagaaactttatggcatactagacttatcaatgaaagcgaggtaatctc ...cctataaaagatgagaccaaccaacctcatctttttt-cgtatcaaatctaagcaattttagaaactttatggcatactagacttatcaatgaaagcgaggtaatctc Subject: NC_003098_spr1351_spr1352|Phosphoglucomutase:Bacterocin transport accessory protein|POSITIVE:POSITIVE|[1335367,1335476]|110 HSP 1 e-value: 3.0E-37 bit: 155.0 Len: 108 Query Start:4 Query End:111 Subject Strand: POSITIVE Subject Start: 4 Subject End: 110 ...cctataaaagatgagaccaaccaatctcatcnnnnnnncgtatcaaatctaagcaattttagaaactttatggcatactagacttatcaatgaaagcgaggtaatctc ...cctataaaagatgagaccaaccaacctcatctttttt-cgtatcaaatctaagcaattttagaaactttatggcatactagacttatcaatgaaagcgaggtaatctc Subject: NC_003028_SP_1498_SP_1499|phosphoglucomutase:bacterocin transport accessory protein|POSITIVE:POSITIVE|[1407739,1407848]|110 HSP 1 e-value: 3.0E-37 bit: 155.0 Len: 108 Query Start:4 Query End:111 Subject Strand: POSITIVE Subject Start: 4 Subject End: 110 ...cctataaaagatgagaccaaccaatctcatcnnnnnnncgtatcaaatctaagcaattttagaaactttatggcatactagacttatcaatgaaagcgaggtaatctc ...cctataaaagatgagaccaaccaacctcatctttttt-cgtatcaaatctaagcaattttagaaactttatggcatactagacttatcaatgaaagcgaggtaatctc Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CCUAUAAAAGAUGAGACCAACCAAUCUCAUCUUUUUUUCGUAUCAAAUCUAAGCAAUUUUAGAAACUUUAUGGCAUACUAGACUUAUCAAUGAAAGCGAGGUAAUCUC (((..(((((((((((........)))))))))))((((((......((((((....)))))).......(((....))).........))))))...)))....... (-18.40) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GAGAUUACCUCGCUUUCAUUGAUAAGUCUAGUAUGCCAUAAAGUUUCUAAAAUUGCUUAGAUUUGAUACGAAAAAAAGAUGAGAUUGGUUGGUCUCAUCUUUUAUAGG (((.....)))...........(((((((((((....................))).))))))))........(((((((((((((....)))))))))))))..... (-22.85) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 277 100 66 55 17 smit:SMT_774|5end_hypothetical_protein_685231..685461_NEGATIVE 244 68 23 123 72 smit:SMT_907|5end_hypothetical_protein_856054..856284_NEGATIVE 220 78 31 193 153 smit:SMT_939|5end_phosphomethylpyrimidine_kinase_892025..892255_NEGATIVE 217 99 51 188 140 smit:SMT_1629|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1616725..1616955_POSITIVE 217 99 51 188 140 smit:SMT_1240|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1200474..1200704_POSITIVE 217 99 51 188 140 smit:SMT_249|5end_hypothetical_protein_predicted_by_Glimmer/Critica_184551..184781_POSITIVE 216 57 12 129 82 smit:SMT_848|5end_hypothetical_protein_795153..795383_NEGATIVE 208 42 1 110 63 smit:SMT_1677|5end_transcription-repair_coupling_factor_1662112..1662342_NEGATIVE 205 52 11 95 51 smit:SMT_414|5end_K03665_GTP-binding_protein_HflX_357554..357784_POSITIVE 203 40 10 220 188 smit:SMT_261|5end_ImpB/MucB/SamB_family_protein_200160..200390_POSITIVE 202 58 14 108 64 smit:SMT_1260|5end_hypothetical_protein_1225517..1225747_POSITIVE 200 99 54 201 155 smit:SMT_2089|5end_dTDP-4-dehydrorhamnose_3,5-epimerase_related_enzyme_1984987..1985217_POSITIVE 200 99 54 201 155 smit:SMT_291|5end_dTDP-4-dehydrorhamnose_3,5-epimerase_234197..234427_POSITIVE 200 56 14 42 2 smit:SMT_244|5end_PTS_system,_mannose-specific_IIAB_component_181857..182087_NEGATIVE 199 32 1 101 73 smit:SMT_320|5end_alcohol_dehydrogenase,_zinc-containing_259492..259722_NEGATIVE 198 68 27 216 171 smit:SMT_1079|5end_dihydropteroate_synthase_1017659..1017889_NEGATIVE 195 97 65 43 14 smit:SMT_1188|5end_hypothetical_protein_1146653..1146883_NEGATIVE 192 91 53 105 60 smit:SMT_1733|5end_DNA_repair_protein_RecN_1716889..1717119_POSITIVE 192 75 34 77 30 smit:SMT_871|5end_K03311_branched-chain_amino_acid:cation_transporter,_LIVCS_family_820160..820390_NEGATIVE 192 75 34 77 30 smit:SMT_386|5end_branched-chain_amino_acid_transport_system_II_carrier_protein_320353..320583_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 277 100 66 45 7 smit:SMT_775|3end_alpha-acetolactate_decarboxylase_685241..685391_NEGATIVE 265 44 4 95 55 smit:SMT_1604|3end_K05833_putative_ABC_transport_system_ATP-binding_protein_1595163..1595313_POSITIVE 244 68 23 104 53 smit:SMT_908|3end_hypothetical_protein_856073..856223_NEGATIVE 216 57 12 132 85 smit:SMT_849|3end_hypothetical_protein_795150..795300_NEGATIVE 209 43 4 82 33 smit:SMT_346|3end_K01992_ABC-2_type_transport_system_permease_protein_285849..285999_POSITIVE 205 52 11 102 58 smit:SMT_413|3end_tRNA_delta(2)-isopentenylpyrophosphate_transferase_357641..357791_POSITIVE 202 58 14 89 45 smit:SMT_1259|3end_hypothetical_protein_1225578..1225728_POSITIVE 200 55 13 125 71 smit:SMT_317|3end_K03625_N_utilization_substance_protein_B_257475..257625_NEGATIVE 198 68 27 92 47 smit:SMT_1080|3end_CAAX_amino_terminal_protease_family_protein_1017783..1017933_NEGATIVE 195 97 65 33 4 smit:SMT_1189|3end_phosphoribosylaminoimidazole_carboxylase_ATPase_subunit_1146663..1146813_NEGATIVE 192 91 53 98 53 smit:SMT_1732|3end_transcriptional_repressor_protein,_putative_1716962..1717112_POSITIVE 191 49 2 115 62 smit:SMT_304|3end_hypothetical_protein_247583..247733_POSITIVE 191 87 44 43 10 smit:SMT_295|3end_hypothetical_protein_predicted_by_Glimmer/Critica_238179..238329_POSITIVE 190 93 53 134 87 smit:SMT_650|3end_pullulanase_557547..557697_NEGATIVE 189 98 52 44 9 smit:SMT_2106|3end_unknown,_predicted_membrane_protein_(TMS5)_1997914..1998064_POSITIVE 189 98 52 144 109 smit:SMT_1404|3end_lipoate-protein_ligase_A_1382036..1382186_POSITIVE 188 42 9 103 64 smit:SMT_340|3end_chaperone_protein_280566..280716_POSITIVE 187 56 23 36 8 smit:SMT_245|3end_hypothetical_protein_predicted_by_Glimmer/Critica_181863..182013_NEGATIVE 186 40 6 47 1 smit:SMT_903|3end_hypothetical_protein_851945..852095_NEGATIVE 184 55 12 67 30 smit:SMT_2135|3end_dipeptidase_2014754..2014904_NEGATIVE