Origin IGS: tttagatagaaataactccttgagtcatcagggagttattttttatctctatcttaactaaacattttatgaagtttacgtaaaaaacaacataactttaaaagctatcatatcacagtttgagcataaatccgaaaactaccaattagatctatcctcacgttacaaaaagtctgtagcaagcagtatttgacgaacttgataccgtcgcattaaatcgaaagtaaaaagttttgccccctttttgccacctagacaaaaaaagagcccatcatacaggctagaaagcttgatatgataggctttttttaattttgattaacgtactgtacggatacgcattgtgtttgtacgaacagcttctccaactggtacaccagctacgataacgatatcgtcacnnnnnttaattaattaannnnnatgtgttcacttgtaaatcaagaaattttcagaaacgctttgaaaacgaattgtttttttataatactatcataaagttcgttatttagctaacaaaaatataattttaagtaaaaaaataaatcaggaaacgaatctcctgattttacatt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aatgtaaaatcaggagattcgtttcctgatttatttttttacttaaaattatatttttgttagctaaataacgaactttatgatagtattataaaaaaacaattcgttttcaaagcgtttctgaaaatttcttgatttacaagtgaacacatnnnnnttaattaattaannnnngtgacgatatcgttatcgtagctggtgtaccagttggagaagctgttcgtacaaacacaatgcgtatccgtacagtacgttaatcaaaattaaaaaaagcctatcatatcaagctttctagcctgtatgatgggctctttttttgtctaggtggcaaaaagggggcaaaactttttactttcgatttaatgcgacggtatcaagttcgtcaaatactgcttgctacagactttttgtaacgtgaggatagatctaattggtagttttcggatttatgctcaaactgtgatatgatagcttttaaagttatgttgttttttacgtaaacttcataaaatgtttagttaagatagagataaaaaataactccctgatgactcaaggagttatttctatctaaa Mask Tandem Repeat Region ================================================ tttagatagaaataactccttgagtcatcagggagttattttttatctctatcttaactaaacattttatgaagtttacgtaaaaaacaacataactttaaaagctatcatatcacagtttgagcataaatccgaaaactaccaattagatctatcctcacgttacaaaaagtctgtagcaagcagtatttgacgaacttgataccgtcgcattaaatcgaaagtaaaaagttttgccccctttttgccacctagacaaaaaaagagcccatcatacaggctagaaagcttgatatgataggctttttttaattttgattaacgtactgtacggatacgcattgtgtttgtacgaacagcttctccaactggtacaccagctacgataacgatatcgtcacnnnnnttaattaattaannnnnatgtgttcacttgtaaatcaagaaattttcagaaacgctttgaaaacgaattgtttttttataatactatcataaagttcgttatttagctaacaaaaatataattttaagtaaaaaaataaatcaggaaacgaatctcctgattttacatt Find is-nt database================================================ Query_seq: SMT_648:SMT_649|SMT_648:SMT_649:L-lactate dehydrogenase:NADP-dependent glyceraldehyde-3-phosphate dehydrogenase:-><-:555442..556016 575 tttagatagaaataactccttgagtcatcagggagttattttttatctctatcttaactaaacattttatgaagtttacgtaaaaaacaacataactttaaaagctatcatatcacagtttgagcataaatccgaaaactaccaattagatctatcctcacgttacaaaaagtctgtagcaagcagtatttgacgaacttgataccgtcgcattaaatcgaaagtaaaaagttttgccccctttttgccacctagacaaaaaaagagcccatcatacaggctagaaagcttgatatgataggctttttttaattttgattaacgtactgtacggatacgcattgtgtttgtacgaacagcttctccaactggtacaccagctacgataacgatatcgtcacnnnnnttaattaattaannnnnatgtgttcacttgtaaatcaagaaattttcagaaacgctttgaaaacgaattgtttttttataatactatcataaagttcgttatttagctaacaaaaatataattttaagtaaaaaaataaatcaggaaacgaatctcctgattttacatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_648:SMT_649|SMT_648:SMT_649:L-lactate dehydrogenase:NADP-dependent glyceraldehyde-3-phosphate dehydrogenase:-><-:555442..556016 575 tttagatagaaataactccttgagtcatcagggagttattttttatctctatcttaactaaacattttatgaagtttacgtaaaaaacaacataactttaaaagctatcatatcacagtttgagcataaatccgaaaactaccaattagatctatcctcacgttacaaaaagtctgtagcaagcagtatttgacgaacttgataccgtcgcattaaatcgaaagtaaaaagttttgccccctttttgccacctagacaaaaaaagagcccatcatacaggctagaaagcttgatatgataggctttttttaattttgattaacgtactgtacggatacgcattgtgtttgtacgaacagcttctccaactggtacaccagctacgataacgatatcgtcacnnnnnttaattaattaannnnnatgtgttcacttgtaaatcaagaaattttcagaaacgctttgaaaacgaattgtttttttataatactatcataaagttcgttatttagctaacaaaaatataattttaagtaaaaaaataaatcaggaaacgaatctcctgattttacatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_648:SMT_649|SMT_648:SMT_649:L-lactate dehydrogenase:NADP-dependent glyceraldehyde-3-phosphate dehydrogenase:-><-:555442..556016 575 tttagatagaaataactccttgagtcatcagggagttattttttatctctatcttaactaaacattttatgaagtttacgtaaaaaacaacataactttaaaagctatcatatcacagtttgagcataaatccgaaaactaccaattagatctatcctcacgttacaaaaagtctgtagcaagcagtatttgacgaacttgataccgtcgcattaaatcgaaagtaaaaagttttgccccctttttgccacctagacaaaaaaagagcccatcatacaggctagaaagcttgatatgataggctttttttaattttgattaacgtactgtacggatacgcattgtgtttgtacgaacagcttctccaactggtacaccagctacgataacgatatcgtcacnnnnnttaattaattaannnnnatgtgttcacttgtaaatcaagaaattttcagaaacgctttgaaaacgaattgtttttttataatactatcataaagttcgttatttagctaacaaaaatataattttaagtaaaaaaataaatcaggaaacgaatctcctgattttacatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tttagatagaaataactccttgagtcatcagggagttattttttatctctatcttaactaaacattttatgaagtttacgtaaaaaacaacataactttaaaagctatcatatcacagtttgagcataaatccgaaaactaccaattagatctatcctcacgttacaaaaagtctgtagcaagcagtatttgacgaacttgataccgtcgcattaaatcgaaagtaaaaagttttgccccctttttgccacctagacaaaaaaagagcccatcatacaggctagaaagcttgatatgataggctttttttaattttgattaacgtactgtacggatacgcattgtgtttgtacgaacagcttctccaactggtacaccagctacgataacgatatcgtcacnnnnnttaattaattaannnnnatgtgttcacttgtaaatcaagaaattttcagaaacgctttgaaaacgaattgtttttttataatactatcataaagttcgttatttagctaacaaaaatataattttaagtaaaaaaataaatcaggaaacgaatctcctgattttacatt Predict ORF larger than 30AA ================================================ Protein_Len: 33 Strand: + Start: 92 End: 190 ........................................................................................... M T L K A I I S Q F E H K S E N Y Q L D L S S R Y K K S V A S S I ................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 32 Strand: + Start: 414 End: 509 ............................................................................................................................................................................................................................................................................................................................................................................................................................. M X X X V F T C K S R N F Q K R F E N E L F F Y N T I I K F V I .................................................................. Protein_Len: 30 Strand: - Start: 322 End: 411 ................................................................................................................................................................................................................................................................................................................................. R V T R I R M T N T R V A E G V P V G A V I V I D D X X X M .................................................................................................................................................................... Protein_Len: 43 Strand: - Start: 166 End: 294 ..................................................................................................................................................................... L F D T A L L I Q R V Q Y R R M L D F T F L K A G K K G G L C F F L G D Y L S S L K M ......................................................................................................................................................................................................................................................................................... Protein_Len: 33 Strand: - Start: 161 End: 259 ................................................................................................................................................................ T V F L R Y C A T N S S S S V T A N F R F Y F T K G G K Q W R S M ............................................................................................................................................................................................................................................................................................................................ Protein_Len: 31 Strand: - Start: 111 End: 203 .............................................................................................................. I V T Q A Y I R F S G I L D I R V N C F T Q L L C Y K V F K M .................................................................................................................................................................................................................................................................................................................................................................................... tttagatagaaataactccttgagtcatcagggagttattttttatctctatcttaactaaacattttatgaagtttacgtaaaaaacaacataactttaaaagctatcatatcacagtttgagcataaatccgaaaactaccaattagatctatcctcacgttacaaaaagtctgtagcaagcagtatttgacgaacttgataccgtcgcattaaatcgaaagtaaaaagttttgccccctttttgccacctagacaaaaaaagagcccatcatacaggctagaaagcttgatatgataggctttttttaattttgattaacgtactgtacggatacgcattgtgtttgtacgaacagcttctccaactggtacaccagctacgataacgatatcgtcacnnnnnttaattaattaannnnnatgtgttcacttgtaaatcaagaaattttcagaaacgctttgaaaacgaattgtttttttataatactatcataaagttcgttatttagctaacaaaaatataattttaagtaaaaaaataaatcaggaaacgaatctcctgattttacatt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tttagatagaaataactccttgagtcatcagggagttattttttatctctatcttaactaaacattttatgaagtttacgtaaaaaacaacataactttaaaagctatcatatcacagtttgagcataaatccgaaaactaccaattagatctatcctcacgttacaaaaagtctgtagcaagcagtatttgacgaacttgataccgtcgcattaaatcgaaagtaaaaagttttgccccctttttgccacctagacaaaaaaagagcccatcatacaggctagaaagcttgatatgataggctttttttaattttgattaacgtactgtacggatacgcattgtgtttgtacgaacagcttctccaactggtacaccagctacgataacgatatcgtcacnnnnnttaattaattaannnnnatgtgttcacttgtaaatcaagaaattttcagaaacgctttgaaaacgaattgtttttttataatactatcataaagttcgttatttagctaacaaaaatataattttaagtaaaaaaataaatcaggaaacgaatctcctgattttacatt Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 100 HP_score: -7.0 Tail_Score: -5.72616 Start: 265 End: 305 Full_Region: aatcaaaattaaaaa aagcctatcatatcaagct ttct agcctgtatgatgggctc tttttttgtctaggt ........................................................................................................................................................................................................................................................................aagcctatcatatcaagctttctagcctgtatgatgggctc.............................................................................................................................................................................................................................................................................. TransTerm Strand: + Conf: 100 HP_score: -9.6 Tail_Score: -5.79588 Start: 267 End: 303 Full_Region: CTAGACAAAAAAAGA GCCCATCATACAGGCT AGAA AGCTTGATATGATAGGC TTTTTTTAATTTTGA ..........................................................................................................................................................................................................................................................................GCCCATCATACAGGCTAGAAAGCTTGATATGATAGGC................................................................................................................................................................................................................................................................................ TransTerm Strand: - Conf: 91 HP_score: -9.6 Tail_Score: -4.50122 Start: 267 End: 303 Full_Region: tcaaaattaaaaaaa gcctatcatatcaagct ttct agcctgtatgatgggc tctttttttgtctag ..........................................................................................................................................................................................................................................................................gcctatcatatcaagctttctagcctgtatgatgggc................................................................................................................................................................................................................................................................................ Find igs database================================================ Query_seq: SMT_648:SMT_649|SMT_648:SMT_649:L-lactate dehydrogenase:NADP-dependent glyceraldehyde-3-phosphate dehydrogenase:-><-:555442..556016 575 tttagatagaaataactccttgagtcatcagggagttattttttatctctatcttaactaaacattttatgaagtttacgtaaaaaacaacataactttaaaagctatcatatcacagtttgagcataaatccgaaaactaccaattagatctatcctcacgttacaaaaagtctgtagcaagcagtatttgacgaacttgataccgtcgcattaaatcgaaagtaaaaagttttgccccctttttgccacctagacaaaaaaagagcccatcatacaggctagaaagcttgatatgataggctttttttaattttgattaacgtactgtacggatacgcattgtgtttgtacgaacagcttctccaactggtacaccagctacgataacgatatcgtcacnnnnnttaattaattaannnnnatgtgttcacttgtaaatcaagaaattttcagaaacgctttgaaaacgaattgtttttttataatactatcataaagttcgttatttagctaacaaaaatataattttaagtaaaaaaataaatcaggaaacgaatctcctgattttacatt Intra-Species Hit: Count: 1 Min: 1 Max: 575 Len: 575 Subject: smit_SMT_648_SMT_649|L-lactate dehydrogenase:NADP-dependent glyceraldehyde-3-phosphate dehydrogenase|POSITIVE:NEGATIVE|[555442,556016]|575 HSP 1 e-value: 0.0 bit: 914.0 Len: 575 Query Start:1 Query End:575 Subject Strand: POSITIVE Subject Start: 1 Subject End: 575 tttagatagaaataactccttgagtcatcagggagttattttttatctctatcttaactaaacattttatgaagtttacgtaaaaaacaacataactttaaaagctatcatatcacagtttgagcataaatccgaaaactaccaattagatctatcctcacgttacaaaaagtctgtagcaagcagtatttgacgaacttgataccgtcgcattaaatcgaaagtaaaaagttttgccccctttttgccacctagacnnnnnnngagcccatcatacaggctagaaagcttgatatgataggcnnnnnnnaattttgattaacgtactgtacggatacgcattgtgtttgtacgaacagcttctccaactggtacaccagctacgataacgatatcgtcacnnnnnttaattaattaannnnnatgtgttcacttgtaaatcaagaaattttcagaaacgctttgaaaacgaattgnnnnnnnataatactatcataaagttcgttatttagctaacaaaaatataattttaagtnnnnnnntaaatcaggaaacgaatctcctgattttacatt tttagatagaaataactccttgagtcatcagggagttattttttatctctatcttaactaaacattttatgaagtttacgtaaaaaacaacataactttaaaagctatcatatcacagtttgagcataaatccgaaaactaccaattagatctatcctcacgttacaaaaagtctgtagcaagcagtatttgacgaacttgataccgtcgcattaaatcgaaagtaaaaagttttgccccctttttgccacctagacaaaaaaagagcccatcatacaggctagaaagcttgatatgataggctttttttaattttgattaacgtactgtacggatacgcattgtgtttgtacgaacagcttctccaactggtacaccagctacgataacgatatcgtcacnnnnnttaattaattaannnnnatgtgttcacttgtaaatcaagaaattttcagaaacgctttgaaaacgaattgtttttttataatactatcataaagttcgttatttagctaacaaaaatataattttaagtaaaaaaataaatcaggaaacgaatctcctgattttacatt Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUUAGAUAGAAAUAACUCCUUGAGUCAUCAGGGAGUUAUUUUUUAUCUCUAUCUUAACUAAACAUUUUAUGAAGUUUACGUAAAAAACAACAUAACUUUAAAAGCUAUCAUAUCACAGUUUGAGCAUAAAUCCGAAAACUACCAAUUAGAUCUAUCCUCACGUUACAAAAAGUCUGUAGCAAGCAGUAUUUGACGAACUUGAUACCGUCGCAUUAAAUCGAAAGUAAAAAGUUUUGCCCCCUUUUUGCCACCUAGACAAAAAAAGAGCCCAUCAUACAGGCUAGAAAGCUUGAUAUGAUAGGCUUUUUUUAAUUUUGAUUAACGUACUGUACGGAUACGCAUUGUGUUUGUACGAACAGCUUCUCCAACUGGUACACCAGCUACGAUAACGAUAUCGUCACNNNNNUUAAUUAAUUAANNNNNAUGUGUUCACUUGUAAAUCAAGAAAUUUUCAGAAACGCUUUGAAAACGAAUUGUUUUUUUAUAAUACUAUCAUAAAGUUCGUUAUUUAGCUAACAAAAAUAUAAUUUUAAGUAAAAAAAUAAAUCAGGAAACGAAUCUCCUGAUUUUACAUU ...((((((((((((((((((((....))))))))))).)))))))))....(((((......((((((((((((......(((((((((..........(((((...............((((((((...........(((((.((.((.........(((((((((((.((((((.(((((.((........((((((..(((..(((........)))..)))..))))))......)).))))).))..))))..((((((((((.((((((((((((....)))))).)))))).))))))))))..)))))..))))))..(((((((((((.....))))))))))).........)).)).)))))........((((((....))))))..........................))))))))((((.....))))..............))))).........)))))))))......))).)))))))))..((((......))))............))))).........(((((((((........)))))))))...... (-133.75) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAUGUAAAAUCAGGAGAUUCGUUUCCUGAUUUAUUUUUUUACUUAAAAUUAUAUUUUUGUUAGCUAAAUAACGAACUUUAUGAUAGUAUUAUAAAAAAACAAUUCGUUUUCAAAGCGUUUCUGAAAAUUUCUUGAUUUACAAGUGAACACAUNNNNNUUAAUUAAUUAANNNNNGUGACGAUAUCGUUAUCGUAGCUGGUGUACCAGUUGGAGAAGCUGUUCGUACAAACACAAUGCGUAUCCGUACAGUACGUUAAUCAAAAUUAAAAAAAGCCUAUCAUAUCAAGCUUUCUAGCCUGUAUGAUGGGCUCUUUUUUUGUCUAGGUGGCAAAAAGGGGGCAAAACUUUUUACUUUCGAUUUAAUGCGACGGUAUCAAGUUCGUCAAAUACUGCUUGCUACAGACUUUUUGUAACGUGAGGAUAGAUCUAAUUGGUAGUUUUCGGAUUUAUGCUCAAACUGUGAUAUGAUAGCUUUUAAAGUUAUGUUGUUUUUUACGUAAACUUCAUAAAAUGUUUAGUUAAGAUAGAGAUAAAAAAUAACUCCCUGAUGACUCAAGGAGUUAUUUCUAUCUAAA ......(((((((((((....)))))))))))...................(((((((((((((((((((......(((((((.(((.((((.((((((((((..((((....(((((.(((((((((..((((.....))))...............................((.((((((....)))))).)).....(((((((((((....((((((............((((....))))...(((((((..(((((..(((((.(((((((((((.((.(((....))).))))))))))))).)))))..((((..(((((((..((..(((..(((((..((((.(((........))).))))..))))).))).....))..))))))))))).)))))))))))).)))))).))))))))))))))))))))...))))).)))).........((((((.....))))))))))))))))..)))))))))))))).)))))))))..))))))))))..((((((((((.(((....))).))))))))))......... (-149.40) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 322 399 357 147 92 smit:SMT_1454|5end_cytidine_deaminase_1437886..1438116_NEGATIVE 312 399 356 224 178 smit:SMT_877|5end_hypothetical_protein_824412..824642_NEGATIVE 302 212 174 71 38 smit:SMT_1921|5end_D-alanine-activating_enzyme_1863185..1863415_POSITIVE 300 209 161 216 153 smit:SMT_1771|5end_unknown,_predicted_membrane_protein_(TMS5)_1747437..1747667_POSITIVE 300 378 331 88 44 smit:SMT_303|5end_hypothetical_protein_245364..245594_POSITIVE 292 209 161 215 153 smit:SMT_2106|5end_unknown,_predicted_membrane_protein_(TMS5)_1997163..1997393_POSITIVE 292 364 321 106 64 smit:SMT_1543|5end_diphosphomevalonate_decarboxylase_1528024..1528254_NEGATIVE 292 366 327 167 126 smit:SMT_1296|5end_K03696_ATP-dependent_Clp_protease_ATP-binding_subunit_ClpC_1257290..1257520_POSITIVE 291 380 332 78 30 smit:SMT_1559|5end_6-phosphofructokinase_1545429..1545659_NEGATIVE 286 392 351 224 181 smit:SMT_1566|5end_K03475_PTS_system,_unknown_pentitol_phosphotransferase_enzyme_IIC_component_1557918..1558148_POSITIVE 284 395 357 78 37 smit:SMT_1375|5end_putative_glucose-inhibited_division_protein_1354438..1354668_NEGATIVE 283 280 236 157 96 smit:SMT_962|5end_putative_hemin_transport_system_ATP-binding_protein_fecE_908584..908814_NEGATIVE 281 378 332 148 75 smit:SMT_320|5end_alcohol_dehydrogenase,_zinc-containing_259492..259722_NEGATIVE 278 400 352 210 158 smit:SMT_178|5end_50S_ribosomal_protein_L29_119161..119391_POSITIVE 275 368 322 159 111 smit:SMT_2168|5end_threonyl-tRNA_synthetase_2040991..2041221_POSITIVE 274 270 228 185 140 smit:SMT_1114|5end_competence_protein_1052555..1052785_NEGATIVE 274 290 241 70 16 smit:SMT_304|5end_hypothetical_protein_246580..246810_POSITIVE 271 310 266 59 10 smit:SMT_1020|5end_transcriptional_regulator,_TetR_family_955755..955985_POSITIVE 270 307 269 82 51 smit:SMT_1928|5end_O-methyltransferase_family_protein_1869652..1869882_NEGATIVE 267 302 263 115 77 smit:SMT_668|5end_hypothetical_protein_predicted_by_Glimmer/Critica_578155..578385_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 493 307 261 80 34 smit:SMT_605|3end_DNA-binding_protein_HU_519257..519407_POSITIVE 406 48 4 87 43 smit:SMT_648|3end_L-lactate_dehydrogenase_555381..555531_POSITIVE 292 364 321 124 82 smit:SMT_1544|3end_mevalonate_kinase_1528006..1528156_NEGATIVE 271 394 357 151 105 smit:SMT_1455|3end_deoxyribose-phosphate_aldolase_1437873..1438023_NEGATIVE 270 307 269 44 13 smit:SMT_1929|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1869690..1869840_NEGATIVE 268 396 357 151 111 smit:SMT_862|3end_hypothetical_protein_predicted_by_Glimmer/Critica_809191..809341_POSITIVE 266 385 344 142 104 smit:SMT_986|3end_threonyl-tRNA_synthetase_931684..931834_POSITIVE 265 378 332 132 78 smit:SMT_1298|3end_K03811_nicotinamide_mononucleotide_transporter_1261797..1261947_POSITIVE 264 361 327 151 124 smit:SMT_1295|3end_transcriptional_regulator_CtsR_1257368..1257518_POSITIVE 260 379 332 143 97 smit:SMT_1704|3end_D-alanine-D-alanine_ligase_1688554..1688704_POSITIVE 260 290 246 136 85 smit:SMT_813|3end_acetyltransferase,_GNAT_family_721867..722017_NEGATIVE 255 326 283 98 53 smit:SMT_519|3end_glutathione_reductase_443940..444090_NEGATIVE 254 380 342 129 84 smit:SMT_1591|3end_DNA-entry_nuclease_1579468..1579618_POSITIVE 253 400 358 86 44 smit:SMT_2073|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1977074..1977224_POSITIVE 253 195 151 140 90 smit:SMT_1530|3end_K07456_DNA_mismatch_repair_protein_MutS2_1513010..1513160_NEGATIVE 253 270 236 147 94 smit:SMT_963|3end_K02015_iron_complex_transport_system_permease_protein_908586..908736_NEGATIVE 252 43 1 92 50 smit:SMT_269|3end_phospho-N-acetylmuramoyl-pentapeptide-transferas_e_208785..208935_POSITIVE 251 304 262 43 5 smit:SMT_230|3end_hypothetical_protein_predicted_by_Glimmer/Critica_165666..165816_NEGATIVE 250 400 359 44 3 smit:SMT_1587|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1577118..1577268_POSITIVE 245 289 244 120 66 smit:SMT_2076|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1978482..1978632_NEGATIVE