Origin IGS: aataaactcctaaaatttttttacctgataattatatcacaaactttagaattaaggaaacgttttctgtatttcataagagtttattttgaaattcaatctataaaaaatcagtaaaacctaagattttactgatttatct .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 agataaatcagtaaaatcttaggttttactgattttttatagattgaatttcaaaataaactcttatgaaatacagaaaacgtttccttaattctaaagtttgtgatataattatcaggtaaaaaaattttaggagtttatt Mask Tandem Repeat Region ================================================ aataaactcctaaaatttttttacctgataattatatcacaaactttagaattaaggaaacgttttctgtatttcataagagtttattttgaaattcaatctataaaaaatcagtaaaacctaagattttactgatttatct Find is-nt database================================================ Query_seq: SMT_645:SMT_646|SMT_645:SMT_646:hypothetical protein:K07284 sortase A:<-<-:550907..551048 142 aataaactcctaaaatttttttacctgataattatatcacaaactttagaattaaggaaacgttttctgtatttcataagagtttattttgaaattcaatctataaaaaatcagtaaaacctaagattttactgatttatct Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_645:SMT_646|SMT_645:SMT_646:hypothetical protein:K07284 sortase A:<-<-:550907..551048 142 aataaactcctaaaatttttttacctgataattatatcacaaactttagaattaaggaaacgttttctgtatttcataagagtttattttgaaattcaatctataaaaaatcagtaaaacctaagattttactgatttatct Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_645:SMT_646|SMT_645:SMT_646:hypothetical protein:K07284 sortase A:<-<-:550907..551048 142 aataaactcctaaaatttttttacctgataattatatcacaaactttagaattaaggaaacgttttctgtatttcataagagtttattttgaaattcaatctataaaaaatcagtaaaacctaagattttactgatttatct Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aataaactcctaaaatttttttacctgataattatatcacaaactttagaattaaggaaacgttttctgtatttcataagagtttattttgaaattcaatctataaaaaatcagtaaaacctaagattttactgatttatct Predict ORF larger than 30AA ================================================ Protein_Len: 30 Strand: + Start: 25 End: 114 ........................ M I I I S Q T L E L R K R F L Y F I R V Y F E I Q S I K N Q ............................ aataaactcctaaaatttttttacctgataattatatcacaaactttagaattaaggaaacgttttctgtatttcataagagtttattttgaaattcaatctataaaaaatcagtaaaacctaagattttactgatttatct Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: - Score: 85 Start: 31 End: 60 ..............................TTTCCTTAATTCTAAAGTTTGTGATATAAT.................................................................................. Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aataaactcctaaaatttttttacctgataattatatcacaaactttagaattaaggaaacgttttctgtatttcataagagtttattttgaaattcaatctataaaaaatcagtaaaacctaagattttactgatttatct Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: SMT_645:SMT_646|SMT_645:SMT_646:hypothetical protein:K07284 sortase A:<-<-:550907..551048 142 aataaactcctaaaatttttttacctgataattatatcacaaactttagaattaaggaaacgttttctgtatttcataagagtttattttgaaattcaatctataaaaaatcagtaaaacctaagattttactgatttatct Intra-Species Hit: Count: 1 Min: 1 Max: 142 Len: 142 Subject: smit_SMT_645_SMT_646|hypothetical protein:K07284 sortase A|NEGATIVE:NEGATIVE|[550907,551048]|142 HSP 1 e-value: 1.0E-62 bit: 240.0 Len: 142 Query Start:1 Query End:142 Subject Strand: POSITIVE Subject Start: 1 Subject End: 142 aataaactcctaaaannnnnnnacctgataattatatcacaaactttagaattaaggaaacgttttctgtatttcataagagtttattttgaaattcaatctataaaaaatcagtaaaacctaagattttactgatttatct aataaactcctaaaatttttttacctgataattatatcacaaactttagaattaaggaaacgttttctgtatttcataagagtttattttgaaattcaatctataaaaaatcagtaaaacctaagattttactgatttatct Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAUAAACUCCUAAAAUUUUUUUACCUGAUAAUUAUAUCACAAACUUUAGAAUUAAGGAAACGUUUUCUGUAUUUCAUAAGAGUUUAUUUUGAAAUUCAAUCUAUAAAAAAUCAGUAAAACCUAAGAUUUUACUGAUUUAUCU (((((((((................(((((....))))).......(((((....(....)...)))))..........)))))))))...................((((((((((((.......)))))))))))).... (-24.80) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGAUAAAUCAGUAAAAUCUUAGGUUUUACUGAUUUUUUAUAGAUUGAAUUUCAAAAUAAACUCUUAUGAAAUACAGAAAACGUUUCCUUAAUUCUAAAGUUUGUGAUAUAAUUAUCAGGUAAAAAAAUUUUAGGAGUUUAUU .((.(((((((((((((.....))))))))))))).))................((((((((((((.(((.((..(((.....)))..)).))).......(.(((((....))))).)...........)))))))))))) (-28.40) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 713 70 21 161 112 smit:SMT_645|5end_hypothetical_protein_550816..551046_NEGATIVE 223 77 35 91 49 smit:SMT_1169|5end_phosphorylase,_Pnp/UDP_family_1117350..1117580_NEGATIVE 210 114 76 36 2 smit:SMT_327|5end_hypothetical_protein_263953..264183_POSITIVE 208 49 7 142 99 smit:SMT_1592|5end_putative_hemolysin_1579655..1579885_POSITIVE 202 66 21 148 102 smit:SMT_103|5end_adenylosuccinate_synthetase_40766..40996_POSITIVE 193 102 63 150 101 smit:SMT_362|5end_K02519_translation_initiation_factor_IF-2_296230..296460_POSITIVE 193 100 57 120 73 smit:SMT_163|5end_cardiolipin_synthetase_107309..107539_POSITIVE 190 53 13 142 100 smit:SMT_282|5end_hypothetical_protein_224866..225096_POSITIVE 189 78 41 231 190 smit:SMT_274|5end_ABC_transporter_substrate-binding_protein_-_oligopeptide_transport_215221..215451_POSITIVE 188 95 55 34 3 smit:SMT_485|5end_hypothetical_protein_421401..421631_NEGATIVE 184 63 25 202 158 smit:SMT_2065|5end_transcriptional_regulator,_MarR_family_1972665..1972895_POSITIVE 184 63 25 202 158 smit:SMT_1320|5end_transcriptional_regulator,_MarR_family_1290234..1290464_POSITIVE 184 78 42 198 156 smit:SMT_1319|5end_muscus_binding_protein_1284864..1285094_POSITIVE 183 69 23 125 81 smit:SMT_1897|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1848858..1849088_NEGATIVE 183 70 21 157 111 smit:SMT_439|5end_hypothetical_protein_predicted_by_Glimmer/Critica_379387..379617_POSITIVE 179 77 31 200 147 smit:SMT_1325|5end_cell_surface_protein_precursor_1300793..1301023_NEGATIVE 178 49 8 140 104 smit:SMT_953|5end_hypothetical_protein_901576..901806_NEGATIVE 177 50 7 144 102 smit:SMT_1796|5end_dihydroorotate_dehydrogenase_A_1770816..1771046_NEGATIVE 176 68 24 192 155 smit:SMT_415|5end_hypothetical_protein_358785..359015_POSITIVE 175 98 74 229 207 smit:SMT_1073|5end_L-asparaginase_1011209..1011439_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 193 102 63 133 84 smit:SMT_361|3end_hypothetical_protein_296293..296443_POSITIVE 184 78 42 95 53 smit:SMT_1318|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1284841..1284991_POSITIVE 183 69 23 106 62 smit:SMT_1898|3end_aminotransferase_(class_V),_putative_1848877..1849027_NEGATIVE 183 70 21 67 21 smit:SMT_438|3end_hypothetical_protein_predicted_by_Glimmer/Critica_379377..379527_POSITIVE 178 49 8 39 3 smit:SMT_954|3end_cation-transporting_ATPase,_E1-E2_family_901677..901827_NEGATIVE 173 100 52 135 91 smit:SMT_805|3end_hypothetical_protein_predicted_by_Glimmer/Critica_715692..715842_NEGATIVE 172 68 24 61 11 smit:SMT_1026|3end_sucrose_phosphorylase_960873..961023_NEGATIVE 171 72 36 41 1 smit:SMT_347|3end_hypothetical_protein_285881..286031_NEGATIVE 168 133 95 91 54 smit:SMT_1104|3end_K06346_spoIIIJ-associated_protein_1045795..1045945_NEGATIVE 168 97 53 77 27 smit:SMT_612|3end_hypothetical_protein_predicted_by_Glimmer/Critica_523674..523824_POSITIVE 168 97 55 105 71 smit:SMT_239|3end_hypothetical_protein_177788..177938_POSITIVE 165 50 6 42 5 smit:SMT_121|3end_hypothetical_protein_74414..74564_POSITIVE 163 80 37 123 80 smit:SMT_1743|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1725058..1725208_NEGATIVE 163 140 95 101 55 smit:SMT_1480|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1462266..1462416_POSITIVE 163 89 56 76 31 smit:SMT_1329|3end_GTP-binding_protein_CgtA_1304478..1304628_NEGATIVE 162 39 4 119 87 smit:SMT_849|3end_hypothetical_protein_795150..795300_NEGATIVE 161 74 40 122 86 smit:SMT_408|3end_hypothetical_protein_353784..353934_POSITIVE 160 85 44 66 14 smit:SMT_1030|3end_alpha-galactosidase_965667..965817_NEGATIVE 159 86 52 103 66 smit:SMT_1891|3end_putative_dihydrolipoamide_S-acetyltransferase_1845019..1845169_POSITIVE 158 67 31 73 38 smit:SMT_331|3end_endo-beta-N-acetylglucosaminidase_271415..271565_POSITIVE