Origin IGS: gagaaaagatgtggattcagtccaaatgtggcattcgcattgaagaatttacctattttgattttcaaaagagtatatattacaatcagttgatgggattttagaaaga .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tctttctaaaatcccatcaactgattgtaatatatactcttttgaaaatcaaaataggtaaattcttcaatgcgaatgccacatttggactgaatccacatcttttctc Mask Tandem Repeat Region ================================================ gagaaaagatgtggattcagtccaaatgtggcattcgcattgaagaatttacctattttgattttcaaaagagtatatattacaatcagttgatgggattttagaaaga Find is-nt database================================================ Query_seq: SMT_536:SMT_537|SMT_536:SMT_537:oxidoreductase, aldo/keto reductase family:oxidoreductase, aldo/keto reductase family:->->:454804..454912 109 gagaaaagatgtggattcagtccaaatgtggcattcgcattgaagaatttacctattttgattttcaaaagagtatatattacaatcagttgatgggattttagaaaga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_536:SMT_537|SMT_536:SMT_537:oxidoreductase, aldo/keto reductase family:oxidoreductase, aldo/keto reductase family:->->:454804..454912 109 gagaaaagatgtggattcagtccaaatgtggcattcgcattgaagaatttacctattttgattttcaaaagagtatatattacaatcagttgatgggattttagaaaga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_536:SMT_537|SMT_536:SMT_537:oxidoreductase, aldo/keto reductase family:oxidoreductase, aldo/keto reductase family:->->:454804..454912 109 gagaaaagatgtggattcagtccaaatgtggcattcgcattgaagaatttacctattttgattttcaaaagagtatatattacaatcagttgatgggattttagaaaga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 gagaaaagatgtggattcagtccaaatgtggcattcgcattgaagaatttacctattttgattttcaaaagagtatatattacaatcagttgatgggattttagaaaga Predict ORF larger than 30AA ================================================ Protein_Len: 31 Strand: + Start: 9 End: 101 ........ M W I Q S K C G I R I E E F T Y F D F Q K S I Y Y N Q L M G F ........ gagaaaagatgtggattcagtccaaatgtggcattcgcattgaagaatttacctattttgattttcaaaagagtatatattacaatcagttgatgggattttagaaaga Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 85 Start: 57 End: 86 ........................................................TTTGATTTTCAAAAGAGTATATATTACAAT....................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ gagaaaagatgtggattcagtccaaatgtggcattcgcattgaagaatttacctattttgattttcaaaagagtatatattacaatcagttgatgggattttagaaaga Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: SMT_536:SMT_537|SMT_536:SMT_537:oxidoreductase, aldo/keto reductase family:oxidoreductase, aldo/keto reductase family:->->:454804..454912 109 gagaaaagatgtggattcagtccaaatgtggcattcgcattgaagaatttacctattttgattttcaaaagagtatatattacaatcagttgatgggattttagaaaga Intra-Species Hit: Count: 1 Min: 1 Max: 109 Len: 109 Subject: smit_SMT_536_SMT_537|oxidoreductase, aldo/keto reductase family:oxidoreductase, aldo/keto reductase family|POSITIVE:POSITIVE|[454804,454912]|109 HSP 1 e-value: 1.0E-55 bit: 216.0 Len: 109 Query Start:1 Query End:109 Subject Strand: POSITIVE Subject Start: 1 Subject End: 109 gagaaaagatgtggattcagtccaaatgtggcattcgcattgaagaatttacctattttgattttcaaaagagtatatattacaatcagttgatgggattttagaaaga gagaaaagatgtggattcagtccaaatgtggcattcgcattgaagaatttacctattttgattttcaaaagagtatatattacaatcagttgatgggattttagaaaga Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GAGAAAAGAUGUGGAUUCAGUCCAAAUGUGGCAUUCGCAUUGAAGAAUUUACCUAUUUUGAUUUUCAAAAGAGUAUAUAUUACAAUCAGUUGAUGGGAUUUUAGAAAGA ..........((((((((..((..((((((.....)))))))).))))))))...((((((..(((......(((.....))).(((....))).)))..))))))... (-16.30) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UCUUUCUAAAAUCCCAUCAACUGAUUGUAAUAUAUACUCUUUUGAAAAUCAAAAUAGGUAAAUUCUUCAAUGCGAAUGCCACAUUUGGACUGAAUCCACAUCUUUUCUC ((..((((((......((((..((.((((...)))).))..))))...........(((..((((........)))))))...))))))..))................ (-12.50) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 253 36 5 221 191 smit:SMT_798|5end_hypothetical_protein_705788..706018_POSITIVE 239 45 7 54 25 smit:SMT_1139|5end_rRNA_(guanine-N1-)-methyltransferase_1079755..1079985_POSITIVE 229 37 1 68 28 smit:SMT_111|5end_alpha-1,2-mannosidase,_putative_61304..61534_NEGATIVE 227 48 1 61 11 smit:SMT_1123|5end_hypothetical_protein_1059292..1059522_POSITIVE 226 53 11 100 50 smit:SMT_1184|5end_phosphotransferase_system,_mannose/fructose/N-acetylgalactosamine-specific_component_IIB_1142037..1142267_NEGATIVE 221 54 12 50 6 smit:SMT_1374|5end_uridylate_kinase_1353020..1353250_NEGATIVE 220 50 8 57 14 smit:SMT_1934|5end_homoserine_dehydrogenase_1872402..1872632_POSITIVE 211 43 2 216 182 smit:SMT_484|5end_hypothetical_protein_420180..420410_NEGATIVE 207 47 2 181 140 smit:SMT_2028|5end_leader_peptidase_(prepilin_peptidase)_/_N-methyltransferase_1943729..1943959_NEGATIVE 207 47 2 181 140 smit:SMT_1754|5end_leader_peptidase_(prepilin_peptidase)_/_N-methyltransferase_1733022..1733252_NEGATIVE 204 46 7 66 28 smit:SMT_1655|5end_glucose-6-phosphate_1-dehydrogenase_1645021..1645251_NEGATIVE 204 46 7 223 182 smit:SMT_1614|5end_K08998_hypothetical_protein_1601330..1601560_POSITIVE 204 67 21 211 154 smit:SMT_1454|5end_cytidine_deaminase_1437886..1438116_NEGATIVE 200 60 18 179 131 smit:SMT_2067|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1973432..1973662_POSITIVE 200 60 19 222 179 smit:SMT_755|5end_shikimate_5-dehydrogenase_659917..660147_NEGATIVE 198 58 11 78 30 smit:SMT_833|5end_hypothetical_protein_predicted_by_Glimmer/Critica_775362..775592_NEGATIVE 198 53 13 52 13 smit:SMT_476|5end_K03308_neurotransmitter:Na+_symporter,_NSS_family_414134..414364_NEGATIVE 196 60 13 130 67 smit:SMT_2079|5end_single_strand_binding_protein_1980144..1980374_POSITIVE 196 58 19 203 172 smit:SMT_1840|5end_6-phosphogluconate_dehydrogenase,_decarboxylating_1806285..1806515_NEGATIVE 195 43 16 78 48 smit:SMT_1830|5end_galactose-6-phosphate_isomerase_1799338..1799568_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 262 43 7 134 97 smit:SMT_1570|3end_hypothetical_protein_1563250..1563400_NEGATIVE 226 53 11 103 53 smit:SMT_1185|3end_beta-galactosidase_1142034..1142184_NEGATIVE 214 50 2 70 13 smit:SMT_2132|3end_UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-_diaminopimelate--D-alanyl-D-alanine_ligase_2013732..2013882_POSITIVE 208 48 7 135 101 smit:SMT_2156|3end_hypothetical_protein_predicted_by_Glimmer/Critica_2030297..2030447_NEGATIVE 206 38 10 149 118 smit:SMT_1955|3end_carbamoyl-phosphate_synthase_small_chain_1888992..1889142_NEGATIVE 196 58 19 127 96 smit:SMT_1841|3end_hypothetical_protein_1806361..1806511_NEGATIVE 196 58 19 127 96 smit:SMT_303|3end_hypothetical_protein_245758..245908_POSITIVE 188 97 52 141 102 smit:SMT_1572|3end_excinuclease_ABC_subunit_B_1566470..1566620_POSITIVE 188 54 11 103 56 smit:SMT_1001|3end_aspartyl/glutamyl-tRNA(Asn/Gln)_amidotransferase_subunit_A_943707..943857_POSITIVE 188 59 11 85 39 smit:SMT_536|3end_oxidoreductase,_aldo/keto_reductase_family_454743..454893_POSITIVE 186 59 11 57 7 smit:SMT_1611|3end_K05896_segregation_and_condensation_protein_A_1600113..1600263_POSITIVE 186 46 3 73 33 smit:SMT_214|3end_leucyl-tRNA_synthetase_147066..147216_POSITIVE 185 95 57 108 73 smit:SMT_1809|3end_MutT/NUDIX_family_protein_1785134..1785284_POSITIVE 184 49 7 54 7 smit:SMT_1694|3end_GTPase_1677632..1677782_POSITIVE 184 66 32 89 55 smit:SMT_600|3end_ADP-ribose_pyrophosphatase_516932..517082_POSITIVE 182 54 17 127 91 smit:SMT_1426|3end_K03095_SprT-like_protein_1410864..1411014_NEGATIVE 181 47 6 74 34 smit:SMT_2045|3end_K+_transport_system,_NAD-binding_component_1956758..1956908_POSITIVE 180 38 9 123 103 smit:SMT_1595|3end_oligopeptide_transport_system_permease_protein_OppC_1585974..1586124_POSITIVE 179 97 60 115 76 smit:SMT_915|3end_sucrose_operon_repressor_868619..868769_POSITIVE 179 40 8 146 102 smit:SMT_196|3end_30S_ribosomal_protein_S11_127723..127873_POSITIVE