Origin IGS: taggataaaggctgcttgagctcctagggcaatggctggatttgaaatgattgaagtaaataactgagcataatcccccgttccacnnnnnttaattaattaannnnnggcctgagttcattactcaagtcctttttga .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tcaaaaaggacttgagtaatgaactcaggccnnnnnttaattaattaannnnngtggaacgggggattatgctcagttatttacttcaatcatttcaaatccagccattgccctaggagctcaagcagcctttatccta Mask Tandem Repeat Region ================================================ taggataaaggctgcttgagctcctagggcaatggctggatttgaaatgattgaagtaaataactgagcataatcccccgttccacnnnnnttaattaattaannnnnggcctgagttcattactcaagtcctttttga Find is-nt database================================================ Query_seq: SMT_472:SMT_473|SMT_472:SMT_473:K03308 neurotransmitter:Na+ symporter, NSS family:K03308 neurotransmitter:Na+ symporter, NSS family:<-<-:409585..409723 139 taggataaaggctgcttgagctcctagggcaatggctggatttgaaatgattgaagtaaataactgagcataatcccccgttccacnnnnnttaattaattaannnnnggcctgagttcattactcaagtcctttttga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_472:SMT_473|SMT_472:SMT_473:K03308 neurotransmitter:Na+ symporter, NSS family:K03308 neurotransmitter:Na+ symporter, NSS family:<-<-:409585..409723 139 taggataaaggctgcttgagctcctagggcaatggctggatttgaaatgattgaagtaaataactgagcataatcccccgttccacnnnnnttaattaattaannnnnggcctgagttcattactcaagtcctttttga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_472:SMT_473|SMT_472:SMT_473:K03308 neurotransmitter:Na+ symporter, NSS family:K03308 neurotransmitter:Na+ symporter, NSS family:<-<-:409585..409723 139 taggataaaggctgcttgagctcctagggcaatggctggatttgaaatgattgaagtaaataactgagcataatcccccgttccacnnnnnttaattaattaannnnnggcctgagttcattactcaagtcctttttga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taggataaaggctgcttgagctcctagggcaatggctggatttgaaatgattgaagtaaataactgagcataatcccccgttccacnnnnnttaattaattaannnnnggcctgagttcattactcaagtcctttttga Predict ORF larger than 30AA ================================================ Protein_Len: 32 Strand: - Start: 1 End: 96 L I F A A Q A G L A I A P N S I I S T F L Q A Y D G T G X X X M ........................................... taggataaaggctgcttgagctcctagggcaatggctggatttgaaatgattgaagtaaataactgagcataatcccccgttccacnnnnnttaattaattaannnnnggcctgagttcattactcaagtcctttttga Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taggataaaggctgcttgagctcctagggcaatggctggatttgaaatgattgaagtaaataactgagcataatcccccgttccacnnnnnttaattaattaannnnnggcctgagttcattactcaagtcctttttga Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: SMT_472:SMT_473|SMT_472:SMT_473:K03308 neurotransmitter:Na+ symporter, NSS family:K03308 neurotransmitter:Na+ symporter, NSS family:<-<-:409585..409723 139 taggataaaggctgcttgagctcctagggcaatggctggatttgaaatgattgaagtaaataactgagcataatcccccgttccacnnnnnttaattaattaannnnnggcctgagttcattactcaagtcctttttga Intra-Species Hit: Count: 2 Min: 1 Max: 139 Len: 139 Subject: smit_SMT_472_SMT_473|K03308 neurotransmitter:Na+ symporter, NSS family:K03308 neurotransmitter:Na+ symporter, NSS family|NEGATIVE:NEGATIVE|[409585,409723]|139 HSP 1 e-value: 5.0E-52 bit: 204.0 Len: 139 Query Start:1 Query End:139 Subject Strand: POSITIVE Subject Start: 1 Subject End: 139 taggataaaggctgcttgagctcctagggcaatggctggatttgaaatgattgaagtaaataactgagcataatcccccgttccacnnnnnttaattaattaannnnnnncctgagttcattactcaagtcctttttga taggataaaggctgcttgagctcctagggcaatggctggatttgaaatgattgaagtaaataactgagcataatcccccgttccacnnnnnttaattaattaannnnnggcctgagttcattactcaagtcctttttga Subject: smit_SMT_469_SMT_470|K03308 neurotransmitter:Na+ symporter, NSS family:K03308 neurotransmitter:Na+ symporter, NSS family|NEGATIVE:NEGATIVE|[405903,406052]|150 HSP 1 e-value: 2.0E-39 bit: 163.0 Len: 86 Query Start:1 Query End:86 Subject Strand: POSITIVE Subject Start: 1 Subject End: 86 taggataaaggctgcttgagctcctagggcaatggctggatttgaaatgattgaagtaaataactgagcataatcccccgttccac..................................................... taggataaaggctgcttgagctcctagggcaatggctggatttgaaatgatagaagtaaataactgagcataatcccccgttccac..................................................... HSP 2 e-value: 8.0E-8 bit: 58.0 Len: 29 Query Start:111 Query End:139 Subject Strand: POSITIVE Subject Start: 122 Subject End: 150 ..............................................................................................................cctgagttcattactcaagtcctttttga ..............................................................................................................cctgagttcattactcaagtcctttttga Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGGAUAAAGGCUGCUUGAGCUCCUAGGGCAAUGGCUGGAUUUGAAAUGAUUGAAGUAAAUAACUGAGCAUAAUCCCCCGUUCCACNNNNNUUAAUUAAUUAANNNNNGGCCUGAGUUCAUUACUCAAGUCCUUUUUGA .(((((..(((((((....))......((.(((((..(((((....(((.....(((.....)))...)))))))).)))))))........................)))))((((.....))))..)))))...... (-26.90) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UCAAAAAGGACUUGAGUAAUGAACUCAGGCCNNNNNUUAAUUAAUUAANNNNNGUGGAACGGGGGAUUAUGCUCAGUUAUUUACUUCAAUCAUUUCAAAUCCAGCCAUUGCCCUAGGAGCUCAAGCAGCCUUUAUCCUA ....(((((.(((((((......((..(((.......................((((...(((.((..(((...(((.....))).....))).))...)))..)))).)))..))..)))))))...)))))...... (-22.65) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 962 50 1 141 92 smit:SMT_472|5end_K03308_neurotransmitter:Na+_symporter,_NSS_family_409494..409724_NEGATIVE 962 50 1 141 92 smit:SMT_469|5end_K03308_neurotransmitter:Na+_symporter,_NSS_family_405812..406042_NEGATIVE 927 60 11 52 3 smit:SMT_480|5end_K03308_neurotransmitter:Na+_symporter,_NSS_family_417433..417663_NEGATIVE 927 60 11 52 3 smit:SMT_475|5end_K03308_neurotransmitter:Na+_symporter,_NSS_family_412997..413227_NEGATIVE 927 60 11 52 3 smit:SMT_474|5end_K03308_neurotransmitter:Na+_symporter,_NSS_family_411894..412124_NEGATIVE 927 60 11 52 3 smit:SMT_471|5end_K03308_neurotransmitter:Na+_symporter,_NSS_family_408351..408581_NEGATIVE 909 58 11 50 3 smit:SMT_481|5end_K03308_neurotransmitter:Na+_symporter,_NSS_family_418240..418470_NEGATIVE 486 27 1 179 153 smit:SMT_476|5end_K03308_neurotransmitter:Na+_symporter,_NSS_family_414134..414364_NEGATIVE 376 130 92 149 112 smit:SMT_470|5end_K03308_neurotransmitter:Na+_symporter,_NSS_family_407303..407533_NEGATIVE 371 45 3 53 7 smit:SMT_110|5end_alpha-L-fucosidase_57445..57675_POSITIVE 329 36 10 34 2 smit:SMT_310|5end_3-oxoacyl-_251370..251600_POSITIVE 310 45 1 87 22 smit:SMT_2070|5end_K02035_peptide/nickel_transport_system_substrate-binding_protein_1974729..1974959_POSITIVE 303 45 4 110 71 smit:SMT_791|5end_thiamine_biosynthesis_protein_ThiI_702377..702607_NEGATIVE 302 45 10 43 4 smit:SMT_1170|5end_acetyltransferase,_CysE/LacA/LpxA/NodL_family_1118126..1118356_NEGATIVE 300 38 1 58 18 smit:SMT_1173|5end_CMP-N-acetylneuraminate-beta-galactosamide-alpha_-2,3-sialyltransferase_1125620..1125850_NEGATIVE 296 41 3 230 190 smit:SMT_949|5end_glycosyl_transferase,_family_2_897693..897923_NEGATIVE 291 43 8 207 174 smit:SMT_1785|5end_phenylalanyl-tRNA_synthetase_beta_chain_1763277..1763507_NEGATIVE 288 50 4 62 17 smit:SMT_1571|5end_CAAX_amino_terminal_protease_family_protein_1563780..1564010_POSITIVE 287 39 3 231 200 smit:SMT_2041|5end_transcription-repair_coupling_factor_1952518..1952748_NEGATIVE 287 57 11 70 33 smit:SMT_1824|5end_hypothetical_protein_1796126..1796356_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 486 27 1 81 55 smit:SMT_477|3end_K03308_neurotransmitter:Na+_symporter,_NSS_family_414232..414382_NEGATIVE 461 86 61 26 1 smit:SMT_470|3end_K03308_neurotransmitter:Na+_symporter,_NSS_family_405963..406113_NEGATIVE 419 130 92 81 43 smit:SMT_476|3end_K03308_neurotransmitter:Na+_symporter,_NSS_family_413313..413463_NEGATIVE 419 130 92 81 43 smit:SMT_475|3end_K03308_neurotransmitter:Na+_symporter,_NSS_family_412020..412170_NEGATIVE 409 130 92 81 42 smit:SMT_472|3end_K03308_neurotransmitter:Na+_symporter,_NSS_family_408613..408763_NEGATIVE 376 130 92 75 38 smit:SMT_474|3end_K03308_neurotransmitter:Na+_symporter,_NSS_family_410920..411070_NEGATIVE 376 130 92 81 44 smit:SMT_471|3end_K03308_neurotransmitter:Na+_symporter,_NSS_family_407371..407521_NEGATIVE 371 45 3 51 5 smit:SMT_109|3end_beta-galactosidase_57523..57673_POSITIVE 352 130 109 81 60 smit:SMT_469|3end_K03308_neurotransmitter:Na+_symporter,_NSS_family_404931..405081_NEGATIVE 352 130 109 81 60 smit:SMT_462|3end_K03308_neurotransmitter:Na+_symporter,_NSS_family_400414..400564_NEGATIVE 334 57 12 150 95 smit:SMT_1349|3end_K03075_preprotein_translocase_SecG_subunit_1327604..1327754_NEGATIVE 312 50 9 151 113 smit:SMT_1448|3end_serine_hydroxymethyltransferase_1430703..1430853_NEGATIVE 310 45 1 121 56 smit:SMT_2069|3end_K02035_peptide/nickel_transport_system_substrate-binding_protein_1974843..1974993_POSITIVE 309 40 2 134 94 smit:SMT_1806|3end_aminoacylase/N-acyl-L-amino_acid_amidohydrolase/hippurate_hydrolase_1781644..1781794_POSITIVE 307 50 9 77 29 smit:SMT_1140|3end_glycogen_phosphorylase_1081316..1081466_NEGATIVE 303 46 9 73 30 smit:SMT_1477|3end_K02077_zinc/manganese_transport_system_substrate-binding_protein_1459447..1459597_NEGATIVE 300 38 1 61 21 smit:SMT_1174|3end_acylneuraminate_cytidylyltransferase_1125617..1125767_NEGATIVE 293 50 11 47 2 smit:SMT_135|3end_hypothetical_protein_86178..86328_NEGATIVE 283 60 12 66 2 smit:SMT_1588|3end_UDP-N-acetylglucosamine_1-carboxyvinyltransferase_1578425..1578575_POSITIVE 277 70 25 78 23 smit:SMT_1815|3end_K02035_peptide/nickel_transport_system_substrate-binding_protein_1788899..1789049_NEGATIVE