Origin IGS: atgaaactgatagtaacacgggttagggcagtcagcacttccagcannnnnttaattaattaannnnnacgcaacccgcttctccccgcgcgttggccgattcatta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taatgaatcggccaacgcgcggggagaagcgggttgcgtnnnnnttaattaattaannnnntgctggaagtgctgactgccctaacccgtgttactatcagtttcat Mask Tandem Repeat Region ================================================ atgaaactgatagtaacacgggttagggcagtcagcacttccagcannnnnttaattaattaannnnnacgcaacccgcttctccccgcgcgttggccgattcatta Find is-nt database================================================ Query_seq: SMT_463:SMT_464|SMT_463:SMT_464:para-aminobenzoate synthetase component I / 4-amino-4-deoxychorismate lyase:ribonuclease Z:->->:402900..403006 107 atgaaactgatagtaacacgggttagggcagtcagcacttccagcannnnnttaattaattaannnnnacgcaacccgcttctccccgcgcgttggccgattcatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_463:SMT_464|SMT_463:SMT_464:para-aminobenzoate synthetase component I / 4-amino-4-deoxychorismate lyase:ribonuclease Z:->->:402900..403006 107 atgaaactgatagtaacacgggttagggcagtcagcacttccagcannnnnttaattaattaannnnnacgcaacccgcttctccccgcgcgttggccgattcatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_463:SMT_464|SMT_463:SMT_464:para-aminobenzoate synthetase component I / 4-amino-4-deoxychorismate lyase:ribonuclease Z:->->:402900..403006 107 atgaaactgatagtaacacgggttagggcagtcagcacttccagcannnnnttaattaattaannnnnacgcaacccgcttctccccgcgcgttggccgattcatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 atgaaactgatagtaacacgggttagggcagtcagcacttccagcannnnnttaattaattaannnnnacgcaacccgcttctccccgcgcgttggccgattcatta Predict ORF larger than 30AA ================================================ atgaaactgatagtaacacgggttagggcagtcagcacttccagcannnnnttaattaattaannnnnacgcaacccgcttctccccgcgcgttggccgattcatta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ atgaaactgatagtaacacgggttagggcagtcagcacttccagcannnnnttaattaattaannnnnacgcaacccgcttctccccgcgcgttggccgattcatta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: SMT_463:SMT_464|SMT_463:SMT_464:para-aminobenzoate synthetase component I / 4-amino-4-deoxychorismate lyase:ribonuclease Z:->->:402900..403006 107 atgaaactgatagtaacacgggttagggcagtcagcacttccagcannnnnttaattaattaannnnnacgcaacccgcttctccccgcgcgttggccgattcatta Intra-Species Hit: Count: 1 Min: 1 Max: 107 Len: 107 Subject: smit_SMT_463_SMT_464|para-aminobenzoate synthetase component I / 4-amino-4-deoxychorismate lyase:ribonuclease Z|POSITIVE:POSITIVE|[402900,403006]|107 HSP 1 e-value: 1.0E-36 bit: 153.0 Len: 107 Query Start:1 Query End:107 Subject Strand: POSITIVE Subject Start: 1 Subject End: 107 atgaaactgatagtaacacgggttagggcagtcagcacttccagcannnnnttaattaattaannnnnacgcaacccgcttctccccgcgcgttggccgattcatta atgaaactgatagtaacacgggttagggcagtcagcacttccagcannnnnttaattaattaannnnnacgcaacccgcttctccccgcgcgttggccgattcatta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUGAAACUGAUAGUAACACGGGUUAGGGCAGUCAGCACUUCCAGCANNNNNUUAAUUAAUUAANNNNNACGCAACCCGCUUCUCCCCGCGCGUUGGCCGAUUCAUUA (((((.(((((.(.....)..)))))(((.....((.......))..........................((((.(((........))).)))))))..))))).. (-15.90) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUGAAUCGGCCAACGCGCGGGGAGAAGCGGGUUGCGUNNNNNUUAAUUAAUUAANNNNNUGCUGGAAGUGCUGACUGCCCUAACCCGUGUUACUAUCAGUUUCAU ..(((((.(.......(((((((.....((((...(((................................)))...)))).....)))))))........).))))) (-20.41) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 678 100 69 183 152 smit:SMT_1986|5end_mercuric_reductase_1914843..1915073_POSITIVE 678 100 69 216 185 smit:SMT_325|5end_K02029_polar_amino_acid_transport_system_permease_protein_263023..263253_NEGATIVE 547 100 69 228 198 smit:SMT_2045|5end_K+_transport_system,_NAD-binding_component_1956139..1956369_POSITIVE 497 93 69 231 207 smit:SMT_1972|5end_uncharacterized_conserved_phage_related_protein_1903662..1903892_POSITIVE 337 45 3 155 118 smit:SMT_188|5end_LSU_ribosomal_protein_L30P_123505..123735_POSITIVE 317 99 77 74 44 smit:SMT_2052|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1961591..1961821_NEGATIVE 303 60 18 140 98 smit:SMT_1855|5end_K03650_tRNA_modification_GTPase_1819711..1819941_POSITIVE 292 98 70 224 194 smit:SMT_1307|5end_glycerol_uptake_facilitator_protein_1271664..1271894_POSITIVE 288 99 70 37 2 smit:SMT_314|5end_biotin_carboxylase_254277..254507_POSITIVE 287 63 21 166 124 smit:SMT_1742|5end_sodium-dependent_phosphate_transporter_1723553..1723783_POSITIVE 285 46 13 134 103 smit:SMT_2026|5end_K03466_DNA_segregation_ATPase_FtsK/SpoIIIE,_S-DNA-T_family_1942432..1942662_NEGATIVE 285 96 74 29 2 smit:SMT_909|5end_type_I_restriction_enzyme_M_protein_858827..859057_NEGATIVE 282 46 7 90 40 smit:SMT_778|5end_S-adenosylmethionine:tRNA_ribosyltransferase-isomerase_686870..687100_POSITIVE 277 46 8 104 68 smit:SMT_1813|5end_amino_acid_transporter_1787142..1787372_POSITIVE 276 46 2 59 9 smit:SMT_1043|5end_chaperonin_GroEL_985766..985996_NEGATIVE 273 46 12 59 23 smit:SMT_1146|5end_maltose_operon_transcriptional_repressor_1090136..1090366_POSITIVE 263 46 19 184 155 smit:SMT_1668|5end_K+_transport_system,_NAD-binding_component_1658288..1658518_NEGATIVE 263 46 8 56 21 smit:SMT_1139|5end_rRNA_(guanine-N1-)-methyltransferase_1079755..1079985_POSITIVE 262 45 17 162 132 smit:SMT_987|5end_N-acetylmannosamine_kinase_/_transcriptional_regulator_932224..932454_NEGATIVE 261 45 1 188 147 smit:SMT_1573|5end_MutT/NUDIX_family_protein_1566413..1566643_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 488 100 79 22 1 smit:SMT_1971|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1903526..1903676_POSITIVE 483 100 79 22 1 smit:SMT_1852|3end_1,4-alpha-glucan_branching_enzyme_1816467..1816617_NEGATIVE 483 100 79 22 1 smit:SMT_326|3end_K02029_polar_amino_acid_transport_system_permease_protein_263217..263367_NEGATIVE 389 100 84 17 1 smit:SMT_2024|3end_hypothetical_protein_1940690..1940840_POSITIVE 389 100 84 17 1 smit:SMT_1981|3end_K03696_ATP-dependent_Clp_protease_ATP-binding_subunit_ClpC_1912868..1913018_POSITIVE 389 100 84 17 1 smit:SMT_1942|3end_zinc_metalloprotease_ZmpB,_truncated_1878384..1878534_POSITIVE 389 100 84 17 1 smit:SMT_1926|3end_mevalonate_kinase_1868062..1868212_POSITIVE 389 100 84 17 1 smit:SMT_1887|3end_LicC_protein_1841740..1841890_POSITIVE 337 45 3 141 104 smit:SMT_187|3end_30S_ribosomal_protein_S5_123571..123721_POSITIVE 297 63 25 98 54 smit:SMT_1973|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1904878..1905028_NEGATIVE 285 46 1 76 30 smit:SMT_2008|3end_K07485_transposase_1928165..1928315_NEGATIVE 285 96 74 29 2 smit:SMT_910|3end_type_I_restriction_enzyme_R_protein_858827..858977_NEGATIVE 277 46 8 75 39 smit:SMT_1812|3end_K07485_transposase_1787193..1787343_POSITIVE 273 46 12 49 13 smit:SMT_1145|3end_maltodextrose_utilization_protein_MalA_1090206..1090356_POSITIVE 268 99 74 55 21 smit:SMT_955|3end_acyltransferase_family_protein_904703..904853_POSITIVE 262 45 17 144 114 smit:SMT_988|3end_N-acetylneuraminate_lyase_932242..932392_NEGATIVE 257 42 21 34 9 smit:SMT_1669|3end_potassium_uptake_protein_KtrB_1658302..1658452_NEGATIVE 255 45 19 151 116 smit:SMT_1230|3end_hypothetical_protein_1192342..1192492_POSITIVE 254 45 8 151 116 smit:SMT_893|3end_leucine_rich_protein_840442..840592_NEGATIVE 253 100 78 130 102 smit:SMT_942|3end_hypothetical_protein_893528..893678_NEGATIVE