Origin IGS: agaaagtttaggatatttacaaatttgatgaggtaattctcttcgaaaatcaaattcaaaccacgtcagcttcaccttgccgtactcaagtacagcctacggctagcttcctagtttgctctttgattttcattgagcataaatcattcaatgtaaataaaaaaatttcttaacgaaagatagagataaatcgaaatcagtagatcgtataaagtgaaaaggaaagatttcaat .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 attgaaatctttccttttcactttatacgatctactgatttcgatttatctctatctttcgttaagaaatttttttatttacattgaatgatttatgctcaatgaaaatcaaagagcaaactaggaagctagccgtaggctgtacttgagtacggcaaggtgaagctgacgtggtttgaatttgattttcgaagagaattacctcatcaaatttgtaaatatcctaaactttct Mask Tandem Repeat Region ================================================ agaaagtttaggatatttacaaatttgatgaggtaattctcttcgaaaatcaaattcaaaccacgtcagcttcaccttgccgtactcaagtacagcctacggctagcttcctagtttgctctttgattttcattgagcataaatcattcaatgtaaataaaaaaatttcttaacgaaagatagagataaatcgaaatcagtagatcgtataaagtgaaaaggaaagatttcaat Find is-nt database================================================ Query_seq: SMT_442:SMT_443|SMT_442:SMT_443:transcriptional regulator, putative:hydroxyethylthiazole kinase:->->:382461..382694 234 agaaagtttaggatatttacaaatttgatgaggtaattctcttcgaaaatcaaattcaaaccacgtcagcttcaccttgccgtactcaagtacagcctacggctagcttcctagtttgctctttgattttcattgagcataaatcattcaatgtaaataaaaaaatttcttaacgaaagatagagataaatcgaaatcagtagatcgtataaagtgaaaaggaaagatttcaat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_442:SMT_443|SMT_442:SMT_443:transcriptional regulator, putative:hydroxyethylthiazole kinase:->->:382461..382694 234 agaaagtttaggatatttacaaatttgatgaggtaattctcttcgaaaatcaaattcaaaccacgtcagcttcaccttgccgtactcaagtacagcctacggctagcttcctagtttgctctttgattttcattgagcataaatcattcaatgtaaataaaaaaatttcttaacgaaagatagagataaatcgaaatcagtagatcgtataaagtgaaaaggaaagatttcaat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_442:SMT_443|SMT_442:SMT_443:transcriptional regulator, putative:hydroxyethylthiazole kinase:->->:382461..382694 234 agaaagtttaggatatttacaaatttgatgaggtaattctcttcgaaaatcaaattcaaaccacgtcagcttcaccttgccgtactcaagtacagcctacggctagcttcctagtttgctctttgattttcattgagcataaatcattcaatgtaaataaaaaaatttcttaacgaaagatagagataaatcgaaatcagtagatcgtataaagtgaaaaggaaagatttcaat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 agaaagtttaggatatttacaaatttgatgaggtaattctcttcgaaaatcaaattcaaaccacgtcagcttcaccttgccgtactcaagtacagcctacggctagcttcctagtttgctctttgattttcattgagcataaatcattcaatgtaaataaaaaaatttcttaacgaaagatagagataaatcgaaatcagtagatcgtataaagtgaaaaggaaagatttcaat Predict ORF larger than 30AA ================================================ Protein_Len: 52 Strand: + Start: 15 End: 170 .............. M Y K F D E V I L F E N Q I Q T T S A S P C R T Q V Q P T A S F L V C S L I F I E H K S F N V N K K I S ................................................................ agaaagtttaggatatttacaaatttgatgaggtaattctcttcgaaaatcaaattcaaaccacgtcagcttcaccttgccgtactcaagtacagcctacggctagcttcctagtttgctctttgattttcattgagcataaatcattcaatgtaaataaaaaaatttcttaacgaaagatagagataaatcgaaatcagtagatcgtataaagtgaaaaggaaagatttcaat Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ agaaagtttaggatatttacaaatttgatgaggtaattctcttcgaaaatcaaattcaaaccacgtcagcttcaccttgccgtactcaagtacagcctacggctagcttcctagtttgctctttgattttcattgagcataaatcattcaatgtaaataaaaaaatttcttaacgaaagatagagataaatcgaaatcagtagatcgtataaagtgaaaaggaaagatttcaat Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: SMT_442:SMT_443|SMT_442:SMT_443:transcriptional regulator, putative:hydroxyethylthiazole kinase:->->:382461..382694 234 agaaagtttaggatatttacaaatttgatgaggtaattctcttcgaaaatcaaattcaaaccacgtcagcttcaccttgccgtactcaagtacagcctacggctagcttcctagtttgctctttgattttcattgagcataaatcattcaatgtaaataaaaaaatttcttaacgaaagatagagataaatcgaaatcagtagatcgtataaagtgaaaaggaaagatttcaat Intra-Species Hit: Count: 23 Min: 1 Max: 234 Len: 234 Subject: smit_SMT_442_SMT_443|transcriptional regulator, putative:hydroxyethylthiazole kinase|POSITIVE:POSITIVE|[382461,382694]|234 HSP 1 e-value: 1.0E-117 bit: 422.0 Len: 234 Query Start:1 Query End:234 Subject Strand: POSITIVE Subject Start: 1 Subject End: 234 agaaagtttaggatatttacaaatttgatgaggtaattctcttcgaaaatcaaattcaaaccacgtcagcttcaccttgccgtactcaagtacagcctacggctagcttcctagtttgctctttgattttcattgagcataaatcattcaatgtaaatnnnnnnntttcttaacgaaagatagagataaatcgaaatcagtagatcgtataaagtgaaaaggaaagatttcaat agaaagtttaggatatttacaaatttgatgaggtaattctcttcgaaaatcaaattcaaaccacgtcagcttcaccttgccgtactcaagtacagcctacggctagcttcctagtttgctctttgattttcattgagcataaatcattcaatgtaaataaaaaaatttcttaacgaaagatagagataaatcgaaatcagtagatcgtataaagtgaaaaggaaagatttcaat Subject: smit_SMT_1809_SMT_1810|MutT/NUDIX family protein:hypothetical protein|POSITIVE:POSITIVE|[1785195,1785358]|164 HSP 1 e-value: 9.0E-46 bit: 184.0 Len: 105 Query Start:39 Query End:143 Subject Strand: POSITIVE Subject Start: 3 Subject End: 107 ......................................ctcttcgaaaatcaaattcaaaccacgtcagcttcaccttgccgtactcaagtacagcctacggctagcttcctagtttgctctttgattttcattgagcataaa........................................................................................... ......................................ctcttcgaaaatcaaattcaaaccacgtcagcttcgccttgccgtactcaagtacagcctgcggctagcttcctagtttgctctttgattttcattgagtataaa........................................................................................... Subject: smit_SMT_890_SMT_891|K02029 polar amino acid transport system permease protein:oxidoreductase, putative|POSITIVE:POSITIVE|[837753,837989]|237 HSP 1 e-value: 9.0E-46 bit: 184.0 Len: 105 Query Start:39 Query End:143 Subject Strand: POSITIVE Subject Start: 3 Subject End: 107 ......................................ctcttcgaaaatcaaattcaaaccacgtcagcttcaccttgccgtactcaagtacagcctacggctagcttcctagtttgctctttgattttcattgagcataaa........................................................................................... ......................................ctcttcgaaaatcaaattcaaaccacgtcagcttcaccttgccgtactcaagtacagcctgcgtctagcttcctagtttgctctttgattttcattgagtataaa........................................................................................... Subject: smit_SMT_1534_SMT_1535|signal peptidase I:exodeoxyribonuclease V alpha chain|POSITIVE:POSITIVE|[1517973,1518206]|234 HSP 1 e-value: 2.0E-43 bit: 176.0 Len: 109 Query Start:34 Query End:142 Subject Strand: NEGATIVE Subject Start: 97 Subject End: 205 .................................taattctcttcgaaaatcaaattcaaaccacgtcagcttcaccttgccgtactcaagtacagcctacggctagcttcctagtttgctctttgattttcattgagcataa............................................................................................ .................................taatactcttcgaaaatcaaattcaaaccacgtcagtttcgccttgccgtactcaagtacagcctgcggctagcttcctagtttgctctttgattttcattgagtataa............................................................................................ Subject: smit_SMT_482_SMT_483|hypothetical protein predicted by Glimmer/Critica:transcriptional activator TipA, putative|NEGATIVE:POSITIVE|[418627,418966]|340 HSP 1 e-value: 1.0E-41 bit: 170.0 Len: 110 Query Start:33 Query End:142 Subject Strand: POSITIVE Subject Start: 221 Subject End: 330 ................................gtaattctcttcgaaaatcaaattcaaaccacgtcagcttcaccttgccgtactcaagtacagcctacggctagcttcctagtttgctctttgattttcattgagcataa............................................................................................ ................................gtaatactcttcgaaaatcaaattcaaaccacgtcagcgtcgccttaccgtactcaagtacagcctgcggctagcttcctagtttgctctttgattttcattgagtataa............................................................................................ Subject: smit_SMT_2108_SMT_2109|adenylosuccinate lyase:hypothetical protein predicted by Glimmer/Critica|NEGATIVE:NEGATIVE|[1998942,1999150]|209 HSP 1 e-value: 2.0E-40 bit: 167.0 Len: 104 Query Start:39 Query End:142 Subject Strand: NEGATIVE Subject Start: 104 Subject End: 207 ......................................ctcttcgaaaatcaaattcaaaccacgtcagcttcaccttgccgtactcaagtacagcctacggctagcttcctagtttgctctttgattttcattgagcataa............................................................................................ ......................................ctcttcgaaaatcaaattcaaaccacgtcagcttcgccttgccgtactcaagtacagcctgtggctagctttctagtttgctctttgattttcattgagtataa............................................................................................ Subject: smit_SMT_1179_SMT_1180|aldose 1-epimerase:putative tagatose-6-phosphate aldose/ketose isomerase|NEGATIVE:NEGATIVE|[1137945,1138125]|181 HSP 1 e-value: 2.0E-40 bit: 167.0 Len: 104 Query Start:39 Query End:142 Subject Strand: NEGATIVE Subject Start: 76 Subject End: 179 ......................................ctcttcgaaaatcaaattcaaaccacgtcagcttcaccttgccgtactcaagtacagcctacggctagcttcctagtttgctctttgattttcattgagcataa............................................................................................ ......................................ctcttcgaaaatcaaattcaaaccacgtcagcttcgccttgccgtactcaagtacagcctgtggctagctttctagtttgctctttgattttcattgagtataa............................................................................................ Subject: smit_SMT_1139_SMT_1140|rRNA (guanine-N1-)-methyltransferase:glycogen phosphorylase|POSITIVE:NEGATIVE|[1080744,1081405]|662 HSP 1 e-value: 2.0E-40 bit: 167.0 Len: 104 Query Start:39 Query End:142 Subject Strand: NEGATIVE Subject Start: 557 Subject End: 660 ......................................ctcttcgaaaatcaaattcaaaccacgtcagcttcaccttgccgtactcaagtacagcctacggctagcttcctagtttgctctttgattttcattgagcataa............................................................................................ ......................................ctcttcgaaaatcaaattcaaaccacgtcagcgttgccttgccgtactcaagtacagcctgcggctagcttcctagtttgctctttgattttcattgagtataa............................................................................................ Subject: smit_SMT_826_SMT_827|hypothetical protein:hypothetical protein|NEGATIVE:POSITIVE|[750113,750528]|416 HSP 1 e-value: 2.0E-40 bit: 167.0 Len: 104 Query Start:39 Query End:142 Subject Strand: POSITIVE Subject Start: 108 Subject End: 211 ......................................ctcttcgaaaatcaaattcaaaccacgtcagcttcaccttgccgtactcaagtacagcctacggctagcttcctagtttgctctttgattttcattgagcataa............................................................................................ ......................................ctcttcgaaaatcaaattcaaaccacgtcagcttcgccttgccgtactccagtacaacctgcggctagcttcctagtttgctctttgattttcattgagtataa............................................................................................ Subject: smit_SMT_2133_SMT_2134|hypothetical protein:hypothetical protein|NEGATIVE:NEGATIVE|[2014098,2014211]|114 HSP 1 e-value: 8.0E-40 bit: 165.0 Len: 103 Query Start:34 Query End:136 Subject Strand: POSITIVE Subject Start: 12 Subject End: 114 .................................taattctcttcgaaaatcaaattcaaaccacgtcagcttcaccttgccgtactcaagtacagcctacggctagcttcctagtttgctctttgattttcattga.................................................................................................. .................................taatactcttcgaaaatcaaattcaaaccacgtcagcgtcgccttaccgtactcaagtacagcctgcggctagcttcctagtttgctctttgattttcattga.................................................................................................. Subject: smit_SMT_1963_SMT_1964|ribonuclease BN, putative:sodium/hydrogen exchanger family protein|NEGATIVE:NEGATIVE|[1896553,1896685]|133 HSP 1 e-value: 8.0E-40 bit: 165.0 Len: 109 Query Start:34 Query End:142 Subject Strand: NEGATIVE Subject Start: 24 Subject End: 132 .................................taattctcttcgaaaatcaaattcaaaccacgtcagcttcaccttgccgtactcaagtacagcctacggctagcttcctagtttgctctttgattttcattgagcataa............................................................................................ .................................taatactcttcgaaaatcaaattcaaaccacgtcagcgtcgccttaccgtactcaagtacagcctgcggctagcttcctagtttgctctttgattttcatkgagtataa............................................................................................ Subject: smit_SMT_872_SMT_873|hypothetical protein:hypothetical protein|NEGATIVE:NEGATIVE|[820709,820822]|114 HSP 1 e-value: 8.0E-40 bit: 165.0 Len: 103 Query Start:34 Query End:136 Subject Strand: POSITIVE Subject Start: 12 Subject End: 114 .................................taattctcttcgaaaatcaaattcaaaccacgtcagcttcaccttgccgtactcaagtacagcctacggctagcttcctagtttgctctttgattttcattga.................................................................................................. .................................taatactcttcgaaaatcaaattcaaaccacgtcagcgtcgccttaccgtactcaagtacagcctgcggctagcttcctagtttgctctttgattttcattga.................................................................................................. Subject: smit_SMT_798_SMT_799|hypothetical protein:hypothetical protein|POSITIVE:NEGATIVE|[707167,707385]|219 HSP 1 e-value: 8.0E-40 bit: 165.0 Len: 111 Query Start:34 Query End:144 Subject Strand: NEGATIVE Subject Start: 110 Subject End: 219 .................................taattctcttcgaaaatcaaattcaaaccacgtcagcttcaccttgccgtactcaagtacagcctacggctagcttcctagtttgctctttgattttcattgagcataaat.......................................................................................... .................................taatactcttcgaaaatctc-ttcaaaccacgtcagcttcaccttgccgtactcaagtacagcctgcggctagcttcctagtttgctctttgattttcattgagtataaat.......................................................................................... Subject: smit_SMT_384_SMT_385|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[319921,320034]|114 HSP 1 e-value: 8.0E-40 bit: 165.0 Len: 103 Query Start:34 Query End:136 Subject Strand: NEGATIVE Subject Start: 1 Subject End: 103 .................................taattctcttcgaaaatcaaattcaaaccacgtcagcttcaccttgccgtactcaagtacagcctacggctagcttcctagtttgctctttgattttcattga.................................................................................................. .................................taatactcttcgaaaatcaaattcaaaccacgtcagcgtcgccttaccgtactcaagtacagcctgcggctagcttcctagtttgctctttgattttcattga.................................................................................................. Subject: smit_SMT_1393_SMT_1394|hypothetical protein:hypothetical protein predicted by Glimmer/Critica|POSITIVE:POSITIVE|[1369621,1370215]|595 HSP 1 e-value: 1.0E-38 bit: 161.0 Len: 109 Query Start:34 Query End:142 Subject Strand: POSITIVE Subject Start: 150 Subject End: 258 .................................taattctcttcgaaaatcaaattcaaaccacgtcagcttcaccttgccgtactcaagtacagcctacggctagcttcctagtttgctctttgattttcattgagcataa............................................................................................ .................................taatactcttcgaaaatcaaattcaaaccacgtcaacgtcgccttgccgtactcaagtacagcctgcggctagtttcctagtttgctctttgattttcattgagtataa............................................................................................ Subject: smit_SMT_1138_SMT_1139|hypothetical protein:rRNA (guanine-N1-)- methyltransferase|NEGATIVE:POSITIVE|[1079638, 1079894]|257 HSP 1 e-value: 1.0E-38 bit: 161.0 Len: 109 Query Start:34 Query End:142 Subject Strand: NEGATIVE Subject Start: 73 Subject End: 181 .................................taattctcttcgaaaatcaaattcaaaccacgtcagcttcaccttgccgtactcaagtacagcctacggctagcttcctagtttgctctttgattttcattgagcataa............................................................................................ .................................taatactcttcgaaaatcaaattcaaaccacgtcaacgtcgccttgccgtactcaagtacagcctgcggctagtttcctagtttgctctttgattttcattgagtataa............................................................................................ Subject: smit_SMT_1068_SMT_1069|ATP-dependent DNA helicase RecG:acetyl xylan esterase, putative|POSITIVE:POSITIVE|[1009047,1009469]|423 HSP 1 e-value: 1.0E-38 bit: 161.0 Len: 109 Query Start:34 Query End:142 Subject Strand: NEGATIVE Subject Start: 19 Subject End: 127 .................................taattctcttcgaaaatcaaattcaaaccacgtcagcttcaccttgccgtactcaagtacagcctacggctagcttcctagtttgctctttgattttcattgagcataa............................................................................................ .................................taatactcttcgaaaatcaaattcaaaccacgtcagcgtcgccttaccgtactcaagtacagcttgcggctagcttcctagtttgctctttgattttcattgagtataa............................................................................................ Subject: smit_SMT_662_SMT_663|A/G-specific adenine glycosylase:phosphate acetyltransferase|NEGATIVE:NEGATIVE|[573918,574105]|188 HSP 1 e-value: 1.0E-38 bit: 161.0 Len: 105 Query Start:39 Query End:143 Subject Strand: POSITIVE Subject Start: 52 Subject End: 156 ......................................ctcttcgaaaatcaaattcaaaccacgtcagcttcaccttgccgtactcaagtacagcctacggctagcttcctagtttgctctttgattttcattgagcataaa........................................................................................... ......................................ctcttcgaaaatcaaattcaaaccacgtcaacgtcgccttgccgtactcaagtacagcctgcggctagtttcctagtttgctctttgattttcattgagtataaa........................................................................................... Subject: smit_SMT_259_SMT_260|dipeptide/tripeptide permease:phage transcriptional repressor|POSITIVE:POSITIVE|[199242,199609]|368 HSP 1 e-value: 8.0E-37 bit: 155.0 Len: 110 Query Start:34 Query End:143 Subject Strand: POSITIVE Subject Start: 102 Subject End: 211 .................................taattctcttcgaaaatcaaattcaaaccacgtcagcttcaccttgccgtactcaagtacagcctacggctagcttcctagtttgctctttgattttcattgagcataaa........................................................................................... .................................taatactcttcgaaaatcaaattcaaaccgcgtcaacgtcgccttgccgtactcaagtacagcctgcggctagtttcctagtttgctctttgattttcattgagtataaa........................................................................................... Subject: smit_SMT_129_SMT_130|putative glycosyltransferase:capsular polysaccharide biosynthesis protein|POSITIVE:POSITIVE|[80944,81134]|191 HSP 1 e-value: 3.0E-36 bit: 153.0 Len: 109 Query Start:34 Query End:142 Subject Strand: POSITIVE Subject Start: 50 Subject End: 158 .................................taattctcttcgaaaatcaaattcaaaccacgtcagcttcaccttgccgtactcaagtacagcctacggctagcttcctagtttgctctttgattttcattgagcataa............................................................................................ .................................taatgctcttcgaaaatcaaattcaaaccgcgtcaacgtcgccttgccgtactcaagtacagcctgcggctagtttcctagtttgctctttgattttcattgagtataa............................................................................................ Subject: smit_SMT_1766_SMT_1767|sucrose-6-phosphate hydrolase:oxidoreductase, short chain dehydrogenase/reductase family|POSITIVE:NEGATIVE|[1743162,1743332]|171 HSP 1 e-value: 8.0E-34 bit: 145.0 Len: 109 Query Start:34 Query End:142 Subject Strand: POSITIVE Subject Start: 5 Subject End: 113 .................................taattctcttcgaaaatcaaattcaaaccacgtcagcttcaccttgccgtactcaagtacagcctacggctagcttcctagtttgctctttgattttcattgagcataa............................................................................................ .................................taatactcttcgaaaatcaaattcaaaccacgtcagcgtcgcctcaccgtactcaagtacagcttgcgactagcttcctagtttgctctttgattttcattgagtataa............................................................................................ Subject: smit_SMT_763_SMT_764|alpha-amylase:alanyl-tRNA synthetase|POSITIVE:NEGATIVE|[669341,669584]|244 HSP 1 e-value: 6.0E-16 bit: 85.7 Len: 51 Query Start:93 Query End:143 Subject Strand: NEGATIVE Subject Start: 83 Subject End: 133 ............................................................................................cagcctacggctagcttcctagtttgctctttgattttcattgagcataaa........................................................................................... ............................................................................................cagcctgcggctagcttcctagtttgctctttgattttcattgagtataaa........................................................................................... HSP 2 e-value: 5.0E-7 bit: 56.0 Len: 52 Query Start:33 Query End:84 Subject Strand: NEGATIVE Subject Start: 190 Subject End: 240 ................................gtaattctcttcgaaaatcaaattcaaaccacgtcagcttcaccttgccgta...................................................................................................................................................... ................................gtaatactcttcgaaaatctc-ttcaaacaacgtcagcttcaccttgccgta...................................................................................................................................................... Subject: smit_SMT_1544_SMT_1545|mevalonate kinase:choline-binding protein|NEGATIVE:NEGATIVE|[1528975,1529249]|275 HSP 1 e-value: 4.0E-11 bit: 69.9 Len: 51 Query Start:34 Query End:84 Subject Strand: NEGATIVE Subject Start: 136 Subject End: 186 .................................taattctcttcgaaaatcaaattcaaaccacgtcagcttcaccttgccgta...................................................................................................................................................... .................................taatactcttcgaaaatcaaattcaaaccacgtcagcgtcgccttaccgta...................................................................................................................................................... HSP 2 e-value: 9.0E-6 bit: 52.0 Len: 34 Query Start:104 Query End:137 Subject Strand: NEGATIVE Subject Start: 36 Subject End: 69 .......................................................................................................tagcttcctagtttgctctttgattttcattgag................................................................................................. .......................................................................................................tagcttcatagtttgctttttgattttcattgag................................................................................................. Inter-species Hit: Count: 18 Min: 12 Max: 234 Len: 223 Subject: NC_003028_SP_1472_SP_1473|oxidoreductase, putative:hypothetical protein|POSITIVE:NEGATIVE|[1386055,1386276]|222 HSP 1 e-value: 4.0E-45 bit: 182.0 Len: 104 Query Start:34 Query End:137 Subject Strand: NEGATIVE Subject Start: 119 Subject End: 222 .................................taattctcttcgaaaatcaaattcaaaccacgtcagcttcaccttgccgtactcaagtacagcctacggctagcttcctagtttgctctttgattttcattgag................................................................................................. .................................taatactcttcgaaaatcaaattcaaaccacgtcagcttcaccttgccgtacttaagtacagcctgcggctagcttcctagtttgctctttgattttcattgag................................................................................................. Subject: NC_003028_SP_1180_SP_1181|ribonucleotide-diphosphate reductase beta subunit:hypothetical protein|POSITIVE:NEGATIVE|[1119540,1119753]|214 HSP 1 e-value: 4.0E-45 bit: 182.0 Len: 104 Query Start:39 Query End:142 Subject Strand: POSITIVE Subject Start: 3 Subject End: 106 ......................................ctcttcgaaaatcaaattcaaaccacgtcagcttcaccttgccgtactcaagtacagcctacggctagcttcctagtttgctctttgattttcattgagcataa............................................................................................ ......................................ctcttcgaaaatcaaattcaaaccacgtcagcttcgccttgccgtactcaagtacagcctgcggctagcttcctagtttgctctttgattttcattgagtataa............................................................................................ Subject: NC_008533_SPD_0622_SPD_0623|transcriptional regulator, TENA/THI-4 family protein, putative:hydroxyethylthiazole kinase|POSITIVE:POSITIVE|[644856,645143]|288 HSP 1 e-value: 1.0E-44 bit: 180.0 Len: 131 Query Start:104 Query End:234 Subject Strand: POSITIVE Subject Start: 157 Subject End: 288 .......................................................................................................tagcttcctagtttgctctttgattttcattgagcataaatcattcaatgtaaatnnnnnnn-tttcttaacgaaagatagagataaatcgaaatcagtagatcgtataaagtgaaaaggaaagatttcaat .......................................................................................................tagcttcctagtttgctctttgattttcattgagtataaatcattcaacgtaaatgaaaaaaatttcttaacgaaagatagagataaatcgaaatcagtagatcgtataaagtgaaaaggaaggatttcaat HSP 2 e-value: 1.0E-10 bit: 67.9 Len: 58 Query Start:12 Query End:69 Subject Strand: POSITIVE Subject Start: 12 Subject End: 68 ...........gatatttacaaatttgatgaggtaattctcttcgaaaatcaaattcaaaccacgtcag..................................................................................................................................................................... ...........gatatttacaaatttgatgaggtaagactcttcgaaaatc-tcttcaaaccacgtcag..................................................................................................................................................................... Subject: NC_003028_SP_0816_SP_0817|hypothetical protein:MutT/nudix family protein|NEGATIVE:NEGATIVE|[768501,768664]|164 HSP 1 e-value: 5.0E-38 bit: 159.0 Len: 108 Query Start:39 Query End:146 Subject Strand: NEGATIVE Subject Start: 55 Subject End: 162 ......................................ctcttcgaaaatcaaattcaaaccacgtcagcttcaccttgccgtactcaagtacagcctacggctagcttcctagtttgctctttgattttcattgagcataaatca........................................................................................ ......................................ctcttcgaaaataaaattcaaaccacgtcagcgtcgccttaccgtactcaagtatagcctgcggctagcttcctagtttgctctttgattttcattgagtataaatca........................................................................................ Subject: NC_008533_SPD_1551_SPD_1552|prevent-host-death family protein:hypothetical protein|NEGATIVE:NEGATIVE|[1574500,1574730]|231 HSP 1 e-value: 5.0E-35 bit: 149.0 Len: 111 Query Start:34 Query End:144 Subject Strand: NEGATIVE Subject Start: 83 Subject End: 193 .................................taattctcttcgaaaatcaaattcaaaccacgtcagcttcaccttgccgtactcaagtacagcctacggctagcttcctagtttgctctttgattttcattgagcataaat.......................................................................................... .................................taatactcttcgaaaatcaaattcaaatcgcgtcaacgtcgccttgccgtactcaagtacagcctgcggctagtttcctagtttgctctttgattttcattgagtataaat.......................................................................................... Subject: NC_003098_spr1586_spr1587|hypothetical protein:hypothetical protein|NEGATIVE:NEGATIVE|[1567002,1567199]|198 HSP 1 e-value: 5.0E-35 bit: 149.0 Len: 111 Query Start:34 Query End:144 Subject Strand: NEGATIVE Subject Start: 50 Subject End: 160 .................................taattctcttcgaaaatcaaattcaaaccacgtcagcttcaccttgccgtactcaagtacagcctacggctagcttcctagtttgctctttgattttcattgagcataaat.......................................................................................... .................................taatactcttcgaaaatcaaattcaaatcgcgtcaacgtcgccttgccgtactcaagtacagcctgcggctagtttcctagtttgctctttgattttcattgagtataaat.......................................................................................... Subject: NC_003028_SP_1741_SP_1742|hypothetical protein:hypothetical protein|NEGATIVE:NEGATIVE|[1645558,1645794]|237 HSP 1 e-value: 5.0E-35 bit: 149.0 Len: 111 Query Start:34 Query End:144 Subject Strand: NEGATIVE Subject Start: 89 Subject End: 199 .................................taattctcttcgaaaatcaaattcaaaccacgtcagcttcaccttgccgtactcaagtacagcctacggctagcttcctagtttgctctttgattttcattgagcataaat.......................................................................................... .................................taatactcttcgaaaatcaaattcaaatcgcgtcaacgtcgccttgccgtactcaagtacagcctgcggctagtttcctagtttgctctttgattttcattgagtataaat.......................................................................................... Subject: NC_008533_SPD_0546_SPD_0547|branched-chain amino acid transport system II carrier protein:hypothetical protein|POSITIVE:POSITIVE|[559539,559741]|203 HSP 1 e-value: 2.0E-34 bit: 147.0 Len: 110 Query Start:34 Query End:143 Subject Strand: POSITIVE Subject Start: 5 Subject End: 114 .................................taattctcttcgaaaatcaaattcaaaccacgtcagcttcaccttgccgtactcaagtacagcctacggctagcttcctagtttgctctttgattttcattgagcataaa........................................................................................... .................................taatactcttcgaaaatcaaattcaaaccgcgtcaacgtcgccttgtcgtactcaagtacagcctgcggctagtttcctagtttgctctttgattttcattgagtataaa........................................................................................... Subject: NC_003098_spr0628_spr0629|hypothetical protein:hydroxyethylthiazole kinase|POSITIVE:POSITIVE|[637325,637591]|267 HSP 1 e-value: 2.0E-34 bit: 147.0 Len: 110 Query Start:104 Query End:213 Subject Strand: POSITIVE Subject Start: 157 Subject End: 267 .......................................................................................................tagcttcctagtttgctctttgattttcattgagcataaatcattcaatgtaaatnnnnnnn-tttcttaacgaaagatagagataaatcgaaatcagtagatcgtataaa..................... .......................................................................................................tagcttcctagtttgctctttgattttcattgagtataaatcattcaacgtaaatgaaaaaaatttcttaacgaaagatagagataaatcgaaatcagtagatcgtataaa..................... HSP 2 e-value: 1.0E-10 bit: 67.9 Len: 58 Query Start:12 Query End:69 Subject Strand: POSITIVE Subject Start: 12 Subject End: 68 ...........gatatttacaaatttgatgaggtaattctcttcgaaaatcaaattcaaaccacgtcag..................................................................................................................................................................... ...........gatatttacaaatttgatgaggtaagactcttcgaaaatc-tcttcaaaccacgtcag..................................................................................................................................................................... Subject: NC_003098_spr0551_spr0552|Branched-chain amino acid transport system carrier protein:hypothetical protein|POSITIVE:POSITIVE|[552009,552211]|203 HSP 1 e-value: 2.0E-34 bit: 147.0 Len: 110 Query Start:34 Query End:143 Subject Strand: POSITIVE Subject Start: 5 Subject End: 114 .................................taattctcttcgaaaatcaaattcaaaccacgtcagcttcaccttgccgtactcaagtacagcctacggctagcttcctagtttgctctttgattttcattgagcataaa........................................................................................... .................................taatactcttcgaaaatcaaattcaaaccgcgtcaacgtcgccttgtcgtactcaagtacagcctgcggctagtttcctagtttgctctttgattttcattgagtataaa........................................................................................... Subject: NC_008533_SPD_0714_SPD_0715|hypothetical protein:MutT/nudix family protein|NEGATIVE:NEGATIVE|[729726,729889]|164 HSP 1 e-value: 3.0E-33 bit: 143.0 Len: 108 Query Start:39 Query End:146 Subject Strand: NEGATIVE Subject Start: 55 Subject End: 162 ......................................ctcttcgaaaatcaaattcaaaccacgtcagcttcaccttgccgtactcaagtacagcctacggctagcttcctagtttgctctttgattttcattgagcataaatca........................................................................................ ......................................ctcttcgaaaataaaattcaaatcacgtaagcgtcgccttaccgtactcaagtatagcctgcggctagcttcctagtttgctctttgattttcattgagtataaatca........................................................................................ Subject: NC_003098_spr0720_spr0721|hypothetical protein:hypothetical protein|NEGATIVE:NEGATIVE|[722195,722358]|164 HSP 1 e-value: 3.0E-33 bit: 143.0 Len: 108 Query Start:39 Query End:146 Subject Strand: NEGATIVE Subject Start: 55 Subject End: 162 ......................................ctcttcgaaaatcaaattcaaaccacgtcagcttcaccttgccgtactcaagtacagcctacggctagcttcctagtttgctctttgattttcattgagcataaatca........................................................................................ ......................................ctcttcgaaaataaaattcaaatcacgtaagcgtcgccttaccgtactcaagtatagcctgcggctagcttcctagtttgctctttgattttcattgagtataaatca........................................................................................ Subject: NC_003028_SP_0716_SP_0717|transcriptional regulator, putative:hydroxyethylthiazole kinase|POSITIVE:POSITIVE|[682792,683027]|236 HSP 1 e-value: 3.0E-18 bit: 93.7 Len: 55 Query Start:104 Query End:158 Subject Strand: POSITIVE Subject Start: 112 Subject End: 166 .......................................................................................................tagcttcctagtttgctctttgattttcattgagcataaatcattcaatgtaaat............................................................................ .......................................................................................................tagcctcctagtttgctctttgattttcattgagtataaatcattcaatgtaaat............................................................................ HSP 2 e-value: 6.0E-16 bit: 85.7 Len: 51 Query Start:184 Query End:234 Subject Strand: POSITIVE Subject Start: 186 Subject End: 236 .......................................................................................................................................................................................agataaatcgaaatcagtagatcgtataaagtgaaaaggaaagatttcaat .......................................................................................................................................................................................agataaatcgaaattagtagatcgtataaagtgaaaaggaaggatttcaat HSP 3 e-value: 1.0E-10 bit: 67.9 Len: 58 Query Start:12 Query End:69 Subject Strand: POSITIVE Subject Start: 12 Subject End: 68 ...........gatatttacaaatttgatgaggtaattctcttcgaaaatcaaattcaaaccacgtcag..................................................................................................................................................................... ...........gatatttacaaatttgatgaggtaagactcttcgaaaatc-tcttcaaaccacgtcag..................................................................................................................................................................... Subject: NC_003028_SP_1717_SP_1718|ABC transporter, ATP-binding protein:hypothetical protein|NEGATIVE:NEGATIVE|[1621815,1622204]|390 HSP 1 e-value: 6.0E-16 bit: 85.7 Len: 47 Query Start:97 Query End:143 Subject Strand: NEGATIVE Subject Start: 113 Subject End: 159 ................................................................................................ctacggctagcttcctagtttgctctttgattttcattgagcataaa........................................................................................... ................................................................................................ctacggctagcttcctagtttgctctttgattttcattgagtataaa........................................................................................... HSP 2 e-value: 2.0E-6 bit: 54.0 Len: 51 Query Start:34 Query End:84 Subject Strand: NEGATIVE Subject Start: 264 Subject End: 313 .................................taattctcttcgaaaatcaaattcaaaccacgtcagcttcaccttgccgta...................................................................................................................................................... .................................taatactcttcgaaaatctc-ttcaaaccacgtcagcttcgccttgccgta...................................................................................................................................................... Subject: NC_003028_SP_0041_SP_0042|bacteriocin BlpU:competence factor transporting ATP-binding/permease protein ComA|POSITIVE:POSITIVE|[40102,40815]|714 HSP 1 e-value: 1.0E-14 bit: 81.8 Len: 45 Query Start:93 Query End:137 Subject Strand: POSITIVE Subject Start: 648 Subject End: 692 ............................................................................................cagcctacggctagcttcctagtttgctctttgattttcattgag................................................................................................. ............................................................................................cagcctacggctagtttcctagtttgctctttgattttcattgag................................................................................................. HSP 2 e-value: 6.0E-13 bit: 75.8 Len: 58 Query Start:27 Query End:84 Subject Strand: POSITIVE Subject Start: 536 Subject End: 593 ..........................gatgaggtaattctcttcgaaaatcaaattcaaaccacgtcagcttcaccttgccgta...................................................................................................................................................... ..........................gatgagttaatactcttcgaaaatcaaattcaaaccacgtcaacgtcgccttgccgta...................................................................................................................................................... Subject: NC_008533_SPD_1528_SPD_1530|ABC transporter, ATP-binding protein:hypothetical protein|NEGATIVE:POSITIVE|[1550754,1552061]|1308 HSP 1 e-value: 1.0E-13 bit: 77.8 Len: 51 Query Start:93 Query End:143 Subject Strand: NEGATIVE Subject Start: 112 Subject End: 162 ............................................................................................cagcctacggctagcttcctagtttgctctttgattttcattgagcataaa........................................................................................... ............................................................................................cagcctacggctagcttcctagttcgctctttgcttttcattgagtataaa........................................................................................... HSP 2 e-value: 9.0E-6 bit: 52.0 Len: 50 Query Start:34 Query End:83 Subject Strand: NEGATIVE Subject Start: 264 Subject End: 312 .................................taattctcttcgaaaatcaaattcaaaccacgtcagcttcaccttgccgt....................................................................................................................................................... .................................taatactcttcgaaaatctc-ttcaaaccacgtcagcttcgccttgccgt....................................................................................................................................................... Subject: NC_003028_SP_2112_SP_2113|maltose operon transcriptional repressor:hypothetical protein|POSITIVE:NEGATIVE|[2026318,2026608]|291 HSP 1 e-value: 9.0E-12 bit: 71.9 Len: 44 Query Start:100 Query End:143 Subject Strand: NEGATIVE Subject Start: 132 Subject End: 175 ...................................................................................................cggctagcttcctagtttgctctttgattttcattgagcataaa........................................................................................... ...................................................................................................cggctagcttcctagtttgcactttgattttcattgagtataaa........................................................................................... HSP 2 e-value: 9.0E-9 bit: 61.9 Len: 51 Query Start:34 Query End:84 Subject Strand: NEGATIVE Subject Start: 238 Subject End: 287 .................................taattctcttcgaaaatcaaattcaaaccacgtcagcttcaccttgccgta...................................................................................................................................................... .................................taatactcttcgaaaatctc-ttcaaaccacgtcagcttcaccttgccgta...................................................................................................................................................... Subject: NC_008533_SPD_0089_SPD_0090|ABC transporter, permease protein:ABC transporter, substrate-binding protein|POSITIVE:POSITIVE|[89674,89930]|257 HSP 1 e-value: 1.0E-10 bit: 67.9 Len: 50 Query Start:93 Query End:142 Subject Strand: POSITIVE Subject Start: 159 Subject End: 208 ............................................................................................cagcctacggctagcttcctagtttgctctttgattttcattgagcataa............................................................................................ ............................................................................................cagcctgcggctagttttctagtttgctctttgattttcattgagtataa............................................................................................ HSP 2 e-value: 9.0E-9 bit: 61.9 Len: 51 Query Start:34 Query End:84 Subject Strand: POSITIVE Subject Start: 52 Subject End: 102 .................................taattctcttcgaaaatcaaattcaaaccacgtcagcttcaccttgccgta...................................................................................................................................................... .................................taatactcttcgaaaatcaaattcaaaccgcgtcaacgtcgccttgccgta...................................................................................................................................................... Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GAUAUUUACAAAUUUGAUGAGGUAAUUCUCUUCGAAAAUCAAAUUCAAACCACGUCAGCUUCACCUUGCCGUACUCAAGUACAGCCUACGGCUAGCUUCCUAGUUUGCUCUUUGAUUUUCAUUGAGCAUAAAUCAUUCAAUGUAAAUAAAAAAAUUUCUUAACGAAAGAUAGAGAUAAAUCGAAAUCAGUAGAUCGUAUAAAGUGAAAAGGAAAGAUUUCAAU ..((((((((...(((((((..((...(((...((((((((((..(((((...(..((((...((..((.((((....)))).))....))..))))..)..)))))...))))))))))...)))..))..))).)))))))))))).....((((((...(....)..)))))).....((((((...........................))))))... (-46.33) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUUGAAAUCUUUCCUUUUCACUUUAUACGAUCUACUGAUUUCGAUUUAUCUCUAUCUUUCGUUAAGAAAUUUUUUUAUUUACAUUGAAUGAUUUAUGCUCAAUGAAAAUCAAAGAGCAAACUAGGAAGCUAGCCGUAGGCUGUACUUGAGUACGGCAAGGUGAAGCUGACGUGGUUUGAAUUUGAUUUUCGAAGAGAAUUACCUCAUCAAAUUUGUAAAUAUC (((((((((...........................))))))))).........((((.....))))........((((((((((((.(((..((..(((..(((((((((((...((((((((..((((.(((....(((((((....)))))))..)))..))))..).)))))))..)))))))))))..)))...))..)))))))...)))))))).. (-60.53) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 912 110 61 208 159 smit:SMT_1535|5end_exodeoxyribonuclease_V_alpha_chain_1518067..1518297_POSITIVE 884 110 61 190 141 smit:SMT_2133|5end_hypothetical_protein_2014007..2014237_NEGATIVE 884 110 61 190 141 smit:SMT_872|5end_hypothetical_protein_820618..820848_NEGATIVE 884 110 61 190 141 smit:SMT_385|5end_hypothetical_protein_319895..320125_POSITIVE 833 110 61 225 176 smit:SMT_662|5end_A/G-specific_adenine_glycosylase_573827..574057_NEGATIVE 771 126 82 95 51 smit:SMT_123|5end_hypothetical_protein_75391..75621_POSITIVE 749 80 31 225 176 smit:SMT_1139|5end_rRNA_(guanine-N1-)-methyltransferase_1079755..1079985_POSITIVE 714 126 82 95 51 smit:SMT_1911|5end_hypothetical_protein_1857614..1857844_POSITIVE 714 126 82 95 51 smit:SMT_1494|5end_nucleoside_diphosphate_kinase_1479500..1479730_POSITIVE 662 126 82 198 154 smit:SMT_740|5end_50S_ribosomal_protein_L10_644116..644346_NEGATIVE 635 126 82 95 51 smit:SMT_2086|5end_hypothetical_protein_1983696..1983926_NEGATIVE 635 126 82 95 51 smit:SMT_867|5end_hypothetical_protein_816316..816546_POSITIVE 555 60 17 231 190 smit:SMT_826|5end_hypothetical_protein_750022..750252_NEGATIVE 547 70 28 214 170 smit:SMT_218|5end_undecaprenyl_pyrophosphate_synthetase_150155..150385_POSITIVE 501 68 23 173 121 smit:SMT_1783|5end_peptide_methionine_sulfoxide_reductase_1759756..1759986_POSITIVE 463 66 28 212 168 smit:SMT_1592|5end_putative_hemolysin_1579655..1579885_POSITIVE 451 70 28 196 155 smit:SMT_236|5end_ribosomal_small_subunit_pseudouridine_synthase_A_174625..174855_POSITIVE 430 73 42 155 124 smit:SMT_604|5end_DNA_polymerase_III,_epsilon_chain_518693..518923_POSITIVE 390 122 85 198 158 smit:SMT_1335|5end_RNA_polymerase_sigma_factor_RpoD_1310201..1310431_NEGATIVE 388 66 28 204 160 smit:SMT_1026|5end_sucrose_phosphorylase_962324..962554_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 884 110 61 75 26 smit:SMT_2134|3end_hypothetical_protein_2014122..2014272_NEGATIVE 884 110 61 75 26 smit:SMT_873|3end_hypothetical_protein_820733..820883_NEGATIVE 884 110 61 75 26 smit:SMT_384|3end_hypothetical_protein_319860..320010_POSITIVE 825 110 61 72 23 smit:SMT_1670|3end_K02030_polar_amino_acid_transport_system_substrate-binding_protein_1659295..1659445_POSITIVE 825 110 61 51 2 smit:SMT_1068|3end_ATP-dependent_DNA_helicase_RecG_1008986..1009136_POSITIVE 771 126 82 88 44 smit:SMT_1075|3end_hypothetical_protein_1013870..1014020_POSITIVE 736 130 81 60 5 smit:SMT_916|3end_3-hydroxy-3-methylglutaryl-CoA_reductase_868730..868880_NEGATIVE 730 127 82 89 44 smit:SMT_1625|3end_DNA_polymerase_IV_1615066..1615216_POSITIVE 714 126 82 55 11 smit:SMT_1910|3end_pyruvate-formate_lyase_activating_enzyme_1857654..1857804_POSITIVE 708 120 71 82 33 smit:SMT_1689|3end_PTS_enzyme,_maltose-and_glucose-specific,_factor_II_homologue_1673139..1673289_POSITIVE 708 126 81 52 7 smit:SMT_663|3end_phosphate_acetyltransferase_574016..574166_NEGATIVE 708 126 82 84 40 smit:SMT_451|3end_thiamine-phosphate_pyrophosphorylase_389647..389797_POSITIVE 692 126 82 74 30 smit:SMT_235|3end_hypothetical_protein_174391..174541_POSITIVE 669 130 82 63 10 smit:SMT_1217|3end_hypothetical_protein_1176166..1176316_NEGATIVE 655 126 89 38 1 smit:SMT_1356|3end_fibronectin-binding_protein-like_protein_A_1334269..1334419_POSITIVE 627 126 84 43 1 smit:SMT_1535|3end_exodeoxyribonuclease_V_alpha_chain_1520513..1520663_POSITIVE 588 70 28 144 103 smit:SMT_123|3end_hypothetical_protein_75623..75773_POSITIVE 586 126 82 88 44 smit:SMT_951|3end_oxidoreductase,_DadA_family_protein/D-amino_acid_oxidase_898995..899145_NEGATIVE 501 68 23 77 25 smit:SMT_1782|3end_hypothetical_protein_1759740..1759890_POSITIVE 392 68 28 77 38 smit:SMT_131|3end_phosphatase,_putative_83031..83181_NEGATIVE