Origin IGS: cactagcaggccgctgtggattgtgtttcacctgtttctatgagtgtacgacaaataatgttcgctgttagataaaacaaaaggaaaattctagtgttttattacaaaacattgacaacattttgatttggtgttatatttacggggagaaatacaaataaaacaagacaactttgagattgattctttggattatggaaattaatagccaagaattagatggtaaatccgctgctgtgtggcgtacggctgttagattaacagcacaggggcgctgtggttggtgatttacatattcttatgaatgtatttcgccaaacgtgcgctgtggataaaactctctattttttgacaggaggataaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tttatcctcctgtcaaaaaatagagagttttatccacagcgcacgtttggcgaaatacattcataagaatatgtaaatcaccaaccacagcgcccctgtgctgttaatctaacagccgtacgccacacagcagcggatttaccatctaattcttggctattaatttccataatccaaagaatcaatctcaaagttgtcttgttttatttgtatttctccccgtaaatataacaccaaatcaaaatgttgtcaatgttttgtaataaaacactagaattttccttttgttttatctaacagcgaacattatttgtcgtacactcatagaaacaggtgaaacacaatccacagcggcctgctagtg Mask Tandem Repeat Region ================================================ cactagcaggccgctgtggattgtgtttcacctgtttctatgagtgtacgacaaataatgttcgctgttagataaaacaaaaggaaaattctagtgttttattacaaaacattgacaacattttgatttggtgttatatttacggggagaaatacaaataaaacaagacaactttgagattgattctttggattatggaaattaatagccaagaattagatggtaaatccgctgctgtgtggcgtacggctgttagattaacagcacaggggcgctgtggttggtgatttacatattcttatgaatgtatttcgccaaacgtgcgctgtggataaaactctctattttttgacaggaggataaa Find is-nt database================================================ Query_seq: SMT_419:SMT_420|SMT_419:SMT_420:hypothetical protein predicted by Glimmer/Critica:hypothetical protein predicted by Glimmer/Critica:->->:362571..362934 364 cactagcaggccgctgtggattgtgtttcacctgtttctatgagtgtacgacaaataatgttcgctgttagataaaacaaaaggaaaattctagtgttttattacaaaacattgacaacattttgatttggtgttatatttacggggagaaatacaaataaaacaagacaactttgagattgattctttggattatggaaattaatagccaagaattagatggtaaatccgctgctgtgtggcgtacggctgttagattaacagcacaggggcgctgtggttggtgatttacatattcttatgaatgtatttcgccaaacgtgcgctgtggataaaactctctattttttgacaggaggataaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_419:SMT_420|SMT_419:SMT_420:hypothetical protein predicted by Glimmer/Critica:hypothetical protein predicted by Glimmer/Critica:->->:362571..362934 364 cactagcaggccgctgtggattgtgtttcacctgtttctatgagtgtacgacaaataatgttcgctgttagataaaacaaaaggaaaattctagtgttttattacaaaacattgacaacattttgatttggtgttatatttacggggagaaatacaaataaaacaagacaactttgagattgattctttggattatggaaattaatagccaagaattagatggtaaatccgctgctgtgtggcgtacggctgttagattaacagcacaggggcgctgtggttggtgatttacatattcttatgaatgtatttcgccaaacgtgcgctgtggataaaactctctattttttgacaggaggataaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_419:SMT_420|SMT_419:SMT_420:hypothetical protein predicted by Glimmer/Critica:hypothetical protein predicted by Glimmer/Critica:->->:362571..362934 364 cactagcaggccgctgtggattgtgtttcacctgtttctatgagtgtacgacaaataatgttcgctgttagataaaacaaaaggaaaattctagtgttttattacaaaacattgacaacattttgatttggtgttatatttacggggagaaatacaaataaaacaagacaactttgagattgattctttggattatggaaattaatagccaagaattagatggtaaatccgctgctgtgtggcgtacggctgttagattaacagcacaggggcgctgtggttggtgatttacatattcttatgaatgtatttcgccaaacgtgcgctgtggataaaactctctattttttgacaggaggataaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 cactagcaggccgctgtggattgtgtttcacctgtttctatgagtgtacgacaaataatgttcgctgttagataaaacaaaaggaaaattctagtgttttattacaaaacattgacaacattttgatttggtgttatatttacggggagaaatacaaataaaacaagacaactttgagattgattctttggattatggaaattaatagccaagaattagatggtaaatccgctgctgtgtggcgtacggctgttagattaacagcacaggggcgctgtggttggtgatttacatattcttatgaatgtatttcgccaaacgtgcgctgtggataaaactctctattttttgacaggaggataaa Predict ORF larger than 30AA ================================================ Protein_Len: 37 Strand: + Start: 174 End: 284 ............................................................................................................................................................................. M R L I L W I M E I N S Q E L D G K S A A V W R T A V R L T A Q G R C G W ................................................................................ Protein_Len: 36 Strand: - Start: 105 End: 212 ........................................................................................................ L V N V V N Q N P T I N V P L F V F L V L C S Q S Q N K P N H F N I A M ........................................................................................................................................................ cactagcaggccgctgtggattgtgtttcacctgtttctatgagtgtacgacaaataatgttcgctgttagataaaacaaaaggaaaattctagtgttttattacaaaacattgacaacattttgatttggtgttatatttacggggagaaatacaaataaaacaagacaactttgagattgattctttggattatggaaattaatagccaagaattagatggtaaatccgctgctgtgtggcgtacggctgttagattaacagcacaggggcgctgtggttggtgatttacatattcttatgaatgtatttcgccaaacgtgcgctgtggataaaactctctattttttgacaggaggataaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 90 Start: 111 End: 140 ..............................................................................................................ATTGACAACATTTTGATTTGGTGTTATATT................................................................................................................................................................................................................................ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ cactagcaggccgctgtggattgtgtttcacctgtttctatgagtgtacgacaaataatgttcgctgttagataaaacaaaaggaaaattctagtgttttattacaaaacattgacaacattttgatttggtgttatatttacggggagaaatacaaataaaacaagacaactttgagattgattctttggattatggaaattaatagccaagaattagatggtaaatccgctgctgtgtggcgtacggctgttagattaacagcacaggggcgctgtggttggtgatttacatattcttatgaatgtatttcgccaaacgtgcgctgtggataaaactctctattttttgacaggaggataaa Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 45 HP_score: -2.2 Tail_Score: -3.33483 Start: 264 End: 280 Full_Region: GCTGTTAGATTAACA GCACAG GGGCG CTGTGG TTGGTGATTTACATA .......................................................................................................................................................................................................................................................................GCACAGGGGCGCTGTGG.................................................................................... TransTerm Strand: + Conf: 51 HP_score: -5.7 Tail_Score: -2.59608 Start: 265 End: 279 Full_Region: CTGTTAGATTAACAG CACAG GGGCG CTGTG GTTGGTGATTTACAT ........................................................................................................................................................................................................................................................................CACAGGGGCGCTGTG..................................................................................... TransTerm Strand: - Conf: 50 HP_score: -5.7 Tail_Score: -2.51547 Start: 265 End: 279 Full_Region: atgtaaatcaccaac cacag cgccc ctgtg ctgttaatctaacag ........................................................................................................................................................................................................................................................................cacagcgcccctgtg..................................................................................... Find igs database================================================ Query_seq: SMT_419:SMT_420|SMT_419:SMT_420:hypothetical protein predicted by Glimmer/Critica:hypothetical protein predicted by Glimmer/Critica:->->:362571..362934 364 cactagcaggccgctgtggattgtgtttcacctgtttctatgagtgtacgacaaataatgttcgctgttagataaaacaaaaggaaaattctagtgttttattacaaaacattgacaacattttgatttggtgttatatttacggggagaaatacaaataaaacaagacaactttgagattgattctttggattatggaaattaatagccaagaattagatggtaaatccgctgctgtgtggcgtacggctgttagattaacagcacaggggcgctgtggttggtgatttacatattcttatgaatgtatttcgccaaacgtgcgctgtggataaaactctctattttttgacaggaggataaa Intra-Species Hit: Count: 1 Min: 1 Max: 364 Len: 364 Subject: smit_SMT_419_SMT_420|hypothetical protein predicted by Glimmer/Critica:hypothetical protein predicted by Glimmer/Critica|POSITIVE:POSITIVE|[362571,362934]|364 HSP 1 e-value: 0.0 bit: 722.0 Len: 364 Query Start:1 Query End:364 Subject Strand: POSITIVE Subject Start: 1 Subject End: 364 cactagcaggccgctgtggattgtgtttcacctgtttctatgagtgtacgacaaataatgttcgctgttagataaaacaaaaggaaaattctagtgttttattacaaaacattgacaacattttgatttggtgttatatttacggggagaaatacaaataaaacaagacaactttgagattgattctttggattatggaaattaatagccaagaattagatggtaaatccgctgctgtgtggcgtacggctgttagattaacagcacaggggcgctgtggttggtgatttacatattcttatgaatgtatttcgccaaacgtgcgctgtggataaaactctctattttttgacaggaggataaa cactagcaggccgctgtggattgtgtttcacctgtttctatgagtgtacgacaaataatgttcgctgttagataaaacaaaaggaaaattctagtgttttattacaaaacattgacaacattttgatttggtgttatatttacggggagaaatacaaataaaacaagacaactttgagattgattctttggattatggaaattaatagccaagaattagatggtaaatccgctgctgtgtggcgtacggctgttagattaacagcacaggggcgctgtggttggtgatttacatattcttatgaatgtatttcgccaaacgtgcgctgtggataaaactctctattttttgacaggaggataaa Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CACUAGCAGGCCGCUGUGGAUUGUGUUUCACCUGUUUCUAUGAGUGUACGACAAAUAAUGUUCGCUGUUAGAUAAAACAAAAGGAAAAUUCUAGUGUUUUAUUACAAAACAUUGACAACAUUUUGAUUUGGUGUUAUAUUUACGGGGAGAAAUACAAAUAAAACAAGACAACUUUGAGAUUGAUUCUUUGGAUUAUGGAAAUUAAUAGCCAAGAAUUAGAUGGUAAAUCCGCUGCUGUGUGGCGUACGGCUGUUAGAUUAACAGCACAGGGGCGCUGUGGUUGGUGAUUUACAUAUUCUUAUGAAUGUAUUUCGCCAAACGUGCGCUGUGGAUAAAACUCUCUAUUUUUUGACAGGAGGAUAAA (((.(((.....))))))..(((((((((.(((((......(((((((((.(((((((((((((.((((.(((((((((..((((...))))..)))))))))....)))).)))..)))))...))))).)).))))))))))))..)))))))))..................(((.(((((((((.((.((((........))))))))))))))).......((((((.((...(..((((.(.(((((((...)))))))...)..))))..)..(((((((..(((((..((....)))))))..)))))))......)).))))))....)))..((((((((....)))))))).. (-95.80) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUUAUCCUCCUGUCAAAAAAUAGAGAGUUUUAUCCACAGCGCACGUUUGGCGAAAUACAUUCAUAAGAAUAUGUAAAUCACCAACCACAGCGCCCCUGUGCUGUUAAUCUAACAGCCGUACGCCACACAGCAGCGGAUUUACCAUCUAAUUCUUGGCUAUUAAUUUCCAUAAUCCAAAGAAUCAAUCUCAAAGUUGUCUUGUUUUAUUUGUAUUUCUCCCCGUAAAUAUAACACCAAAUCAAAAUGUUGUCAAUGUUUUGUAAUAAAACACUAGAAUUUUCCUUUUGUUUUAUCUAACAGCGAACAUUAUUUGUCGUACACUCAUAGAAACAGGUGAAACACAAUCCACAGCGGCCUGCUAGUG ......(((((((......)))).))).......((((((((.(((((((.....(((((((....))..)))))..(((((....(((((((....)))))))...((((..((..(((((.((.((((((...(((((........((((((((..((((.......)))))).)))))).................((((.((((((............)))))).))))..)))))....)))))).((((((((((.((((((((..((.......))..))))))))...)))..)))))))...)).))))).))..))))....)))))........))).))))))..))).))) (-69.90) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 381 287 241 211 157 smit:SMT_1668|5end_K+_transport_system,_NAD-binding_component_1658288..1658518_NEGATIVE 344 287 241 113 45 smit:SMT_388|5end_branched-chain_amino_acid_transport_system_II_carrier_protein_321172..321402_POSITIVE 342 287 241 44 1 smit:SMT_1373|5end_K02838_ribosome_recycling_factor_1352274..1352504_NEGATIVE 338 256 215 61 6 smit:SMT_2141|5end_hypothetical_protein_2019976..2020206_NEGATIVE 338 256 215 61 6 smit:SMT_870|5end_hypothetical_protein_818737..818967_NEGATIVE 334 287 246 177 123 smit:SMT_2016|5end_tyrosine_recombinase_1933425..1933655_NEGATIVE 332 276 233 59 11 smit:SMT_1559|5end_6-phosphofructokinase_1545429..1545659_NEGATIVE 319 278 232 195 160 smit:SMT_857|5end_endo-beta-N-acetylglucosaminidase_802491..802721_NEGATIVE 317 280 231 74 27 smit:SMT_169|5end_30S_ribosomal_protein_S10_114515..114745_POSITIVE 306 275 236 229 169 smit:SMT_197|5end_DNA-directed_RNA_polymerase,_alpha_subunit_127686..127916_POSITIVE 302 290 241 61 16 smit:SMT_916|5end_3-hydroxy-3-methylglutaryl-CoA_reductase_870004..870234_NEGATIVE 301 39 2 97 56 smit:SMT_1075|5end_hypothetical_protein_1013473..1013703_POSITIVE 301 278 231 206 140 smit:SMT_714|5end_hypothetical_protein_predicted_by_Glimmer/Critica_620979..621209_NEGATIVE 299 278 232 191 148 smit:SMT_1873|5end_K02006_cobalt_transport_system_ATP-binding_protein_1831936..1832166_POSITIVE 298 340 294 146 100 smit:SMT_1201|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1163920..1164150_NEGATIVE 297 274 232 217 177 smit:SMT_939|5end_phosphomethylpyrimidine_kinase_892025..892255_NEGATIVE 296 46 6 229 182 smit:SMT_849|5end_hypothetical_protein_796127..796357_NEGATIVE 295 280 234 127 81 smit:SMT_763|5end_alpha-amylase_667866..668096_POSITIVE 294 279 231 69 11 smit:SMT_1015|5end_galactose_operon_repressor_951938..952168_POSITIVE 293 280 232 228 168 smit:SMT_1698|5end_CMP-binding_factor_1680092..1680322_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 324 280 231 83 23 smit:SMT_780|3end_peptidase_family_U32_688752..688902_NEGATIVE 322 280 231 136 81 smit:SMT_2005|3end_pullulanase,_type_I_1925615..1925765_NEGATIVE 322 280 231 136 81 smit:SMT_1944|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1879075..1879225_NEGATIVE 313 277 234 85 41 smit:SMT_481|3end_K03308_neurotransmitter:Na+_symporter,_NSS_family_417641..417791_NEGATIVE 306 254 219 139 104 smit:SMT_1892|3end_hypothetical_protein_1846193..1846343_POSITIVE 302 290 241 61 16 smit:SMT_917|3end_hydroxymethylglutaryl-CoA_synthase_870004..870154_NEGATIVE 300 267 227 54 3 smit:SMT_768|3end_hypothetical_protein_676451..676601_NEGATIVE 298 340 294 121 75 smit:SMT_1202|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1163945..1164095_NEGATIVE 294 74 31 80 30 smit:SMT_632|3end_hypothetical_protein_537274..537424_NEGATIVE 289 270 222 63 11 smit:SMT_2049|3end_3-oxoacyl-_1958736..1958886_NEGATIVE 289 50 1 67 25 smit:SMT_596|3end_K03976_putative_transcription_regulator_512590..512740_NEGATIVE 289 50 9 79 37 smit:SMT_478|3end_hypothetical_protein_predicted_by_Glimmer/Critica_415257..415407_POSITIVE 288 278 231 144 104 smit:SMT_1967|3end_dicarboxylate/amino_acid:cation_(Na+_or_H+)_symporter_(DAACS)_family_protein_1899498..1899648_NEGATIVE 288 278 231 144 104 smit:SMT_996|3end_K03309_dicarboxylate/amino_acid:cation_(Na+_or_H+)_symporter,_DAACS_family_938255..938405_POSITIVE 287 266 231 107 69 smit:SMT_1939|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1876453..1876603_POSITIVE 285 263 226 96 58 smit:SMT_700|3end_putative_signal_recognition_particle_-inhibited_division_protein_610330..610480_NEGATIVE 285 280 231 71 13 smit:SMT_109|3end_beta-galactosidase_57523..57673_POSITIVE 283 279 233 133 79 smit:SMT_921|3end_putative_PP2C_protein_phosphatase_874498..874648_NEGATIVE 282 280 231 60 1 smit:SMT_1821|3end_Mg2+_and_Co2+_transporter_1794515..1794665_POSITIVE 280 290 243 76 22 smit:SMT_1964|3end_sodium/hydrogen_exchanger_family_protein_1896596..1896746_NEGATIVE