Origin IGS: nnnnnagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacagct .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 agctgtttcctgatctaggtgaccatcctagcacagggggatcctctagagtcgacctgcaggcatgcaagctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctnnnnn Mask Tandem Repeat Region ================================================ nnnnnagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacagct Find is-nt database================================================ Query_seq: SMT_387:SMT_388|SMT_387:SMT_388:hypothetical protein predicted by Glimmer/Critica:branched-chain amino acid transport system II carrier protein:->->:321179..321311 133 nnnnnagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacagct Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_387:SMT_388|SMT_387:SMT_388:hypothetical protein predicted by Glimmer/Critica:branched-chain amino acid transport system II carrier protein:->->:321179..321311 133 nnnnnagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacagct Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_387:SMT_388|SMT_387:SMT_388:hypothetical protein predicted by Glimmer/Critica:branched-chain amino acid transport system II carrier protein:->->:321179..321311 133 nnnnnagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacagct Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 nnnnnagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacagct Predict ORF larger than 30AA ================================================ Protein_Len: 31 Strand: + Start: 40 End: 132 ....................................... M A D S L M Q S L H A C R S T L E D P P V L G W S P R S G N S . Protein_Len: 43 Strand: - Start: 3 End: 131 .. X A G L V C V A E G R A N A S E N I C L K C A Q L D V R S S G G T S P H D G L D P F M .. Protein_Len: 38 Strand: - Start: 2 End: 115 . X L A W Y A F R R E G R T P R N M L A S S A H R C T S E L P D G Q A L I T M .................. nnnnnagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacagct Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ nnnnnagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacagct Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: SMT_387:SMT_388|SMT_387:SMT_388:hypothetical protein predicted by Glimmer/Critica:branched-chain amino acid transport system II carrier protein:->->:321179..321311 133 nnnnnagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacagct Intra-Species Hit: Count: 36 Min: 6 Max: 133 Len: 128 Subject: smit_SMT_2029_SMT_2030|acetyltransferase, GNAT family:chromosomal replication initiator protein DnaA|POSITIVE:NEGATIVE|[1944345,1944879]|535 HSP 1 e-value: 6.0E-67 bit: 254.0 Len: 128 Query Start:6 Query End:133 Subject Strand: POSITIVE Subject Start: 353 Subject End: 480 .....agcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacagct .....agcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacagct Subject: smit_SMT_2016_SMT_2017|tyrosine recombinase:exonuclease RexA|NEGATIVE:NEGATIVE|[1933516,1935951]|2436 HSP 1 e-value: 6.0E-67 bit: 254.0 Len: 128 Query Start:6 Query End:133 Subject Strand: NEGATIVE Subject Start: 65 Subject End: 192 .....agcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacagct .....agcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacagct Subject: smit_SMT_2000_SMT_2001|hypothetical protein:membrane protease protein family|NEGATIVE:POSITIVE|[1923199,1923526]|328 HSP 1 e-value: 6.0E-67 bit: 254.0 Len: 128 Query Start:6 Query End:133 Subject Strand: POSITIVE Subject Start: 146 Subject End: 273 .....agcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacagct .....agcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacagct Subject: smit_SMT_1985_SMT_1986|hypothetical protein predicted by Glimmer/Critica:mercuric reductase|NEGATIVE:POSITIVE|[1914662,1914982]|321 HSP 1 e-value: 6.0E-67 bit: 254.0 Len: 128 Query Start:6 Query End:133 Subject Strand: NEGATIVE Subject Start: 144 Subject End: 271 .....agcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacagct .....agcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacagct Subject: smit_SMT_1978_SMT_1979|hypothetical protein:possible cell surface protein|NEGATIVE:NEGATIVE|[1909657,1910371]|715 HSP 1 e-value: 6.0E-67 bit: 254.0 Len: 128 Query Start:6 Query End:133 Subject Strand: POSITIVE Subject Start: 496 Subject End: 623 .....agcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacagct .....agcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacagct Subject: smit_SMT_1967_SMT_1968|dicarboxylate/amino acid:cation (Na+ or H+) symporter (DAACS) family protein:transcription-repair coupling factor|NEGATIVE:NEGATIVE|[1899774,1900040]|267 HSP 1 e-value: 6.0E-67 bit: 254.0 Len: 128 Query Start:6 Query End:133 Subject Strand: NEGATIVE Subject Start: 1 Subject End: 128 .....agcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacagct .....agcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacagct Subject: smit_SMT_1953_SMT_1954|hypothetical protein:6-phospho-beta- glucosidase|POSITIVE:POSITIVE|[1888168,1888687]|520 HSP 1 e-value: 6.0E-67 bit: 254.0 Len: 128 Query Start:6 Query End:133 Subject Strand: POSITIVE Subject Start: 349 Subject End: 476 .....agcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacagct .....agcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacagct Subject: smit_SMT_1946_SMT_1947|sucrose-6-phosphate hydrolase:lysine decarboxylase|POSITIVE:NEGATIVE|[1881266,1882402]|1137 HSP 1 e-value: 6.0E-67 bit: 254.0 Len: 128 Query Start:6 Query End:133 Subject Strand: NEGATIVE Subject Start: 475 Subject End: 602 .....agcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacagct .....agcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacagct Subject: smit_SMT_1944_SMT_1945|hypothetical protein predicted by Glimmer/Critica:putative dihydrolipoamide S-acetyltransferase|NEGATIVE:POSITIVE|[1879585, 1880100]|516 HSP 1 e-value: 6.0E-67 bit: 254.0 Len: 128 Query Start:6 Query End:133 Subject Strand: NEGATIVE Subject Start: 36 Subject End: 163 .....agcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacagct .....agcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacagct Subject: smit_SMT_1895_SMT_1896|hypothetical protein predicted by Glimmer/Critica:lysine decarboxylase|POSITIVE:NEGATIVE|[1847463,1848162]|700 HSP 1 e-value: 6.0E-67 bit: 254.0 Len: 128 Query Start:6 Query End:133 Subject Strand: NEGATIVE Subject Start: 60 Subject End: 187 .....agcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacagct .....agcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacagct Subject: smit_SMT_1885_SMT_1886|hypothetical protein predicted by Glimmer/Critica:hypothetical protein predicted by Glimmer/Critica|NEGATIVE:POSITIVE|[1840148,1841123]|976 HSP 1 e-value: 6.0E-67 bit: 254.0 Len: 128 Query Start:6 Query End:133 Subject Strand: POSITIVE Subject Start: 649 Subject End: 776 .....agcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacagct .....agcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacagct Subject: smit_SMT_1857_SMT_1858|hypothetical protein:K03497 chromosome partitioning protein, ParB family|POSITIVE:NEGATIVE|[1821206,1821776]|571 HSP 1 e-value: 6.0E-67 bit: 254.0 Len: 128 Query Start:6 Query End:133 Subject Strand: NEGATIVE Subject Start: 75 Subject End: 202 .....agcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacagct .....agcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacagct Subject: smit_SMT_1849_SMT_1850|hypothetical protein:K01421 putative membrane protein|POSITIVE:POSITIVE|[1813865,1815201]|1337 HSP 1 e-value: 6.0E-67 bit: 254.0 Len: 128 Query Start:6 Query End:133 Subject Strand: POSITIVE Subject Start: 868 Subject End: 995 .....agcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacagct .....agcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacagct Subject: smit_SMT_1690_SMT_1691|K06148 ATP-binding cassette, subfamily C, bacterial:putative deoxyribonuclease|NEGATIVE:POSITIVE|[1674870, 1675117]|248 HSP 1 e-value: 6.0E-67 bit: 254.0 Len: 128 Query Start:6 Query End:133 Subject Strand: NEGATIVE Subject Start: 1 Subject End: 128 .....agcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacagct .....agcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacagct Subject: smit_SMT_1685_SMT_1686|phenylalanyl-tRNA synthetase alpha chain:hypothetical protein predicted by Glimmer/Critica|NEGATIVE:POSITIVE|[1668936,1670314]|1379 HSP 1 e-value: 6.0E-67 bit: 254.0 Len: 128 Query Start:6 Query End:133 Subject Strand: POSITIVE Subject Start: 1176 Subject End: 1303 .....agcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacagct .....agcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacagct Subject: smit_SMT_779_SMT_780|ribonuclease BN, putative:peptidase family U32|NEGATIVE:NEGATIVE|[688621,688841]|221 HSP 1 e-value: 6.0E-67 bit: 254.0 Len: 128 Query Start:6 Query End:133 Subject Strand: NEGATIVE Subject Start: 1 Subject End: 128 .....agcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacagct .....agcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacagct Subject: smit_SMT_478_SMT_479|hypothetical protein predicted by Glimmer/Critica:K03308 neurotransmitter:Na+ symporter, NSS family|POSITIVE:NEGATIVE|[415318,415693]|376 HSP 1 e-value: 6.0E-67 bit: 254.0 Len: 128 Query Start:6 Query End:133 Subject Strand: POSITIVE Subject Start: 198 Subject End: 325 .....agcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacagct .....agcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacagct Subject: smit_SMT_387_SMT_388|hypothetical protein predicted by Glimmer/Critica:branched-chain amino acid transport system II carrier protein|POSITIVE:POSITIVE|[321179,321311]|133 HSP 1 e-value: 6.0E-67 bit: 254.0 Len: 128 Query Start:6 Query End:133 Subject Strand: POSITIVE Subject Start: 6 Subject End: 133 .....agcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacagct .....agcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacagct Subject: smit_SMT_1966_SMT_1967|DNA polymerase III alpha subunit:dicarboxylate/amino acid:cation (Na+ or H+) symporter (DAACS) family protein|POSITIVE:NEGATIVE|[1898389,1899587]|1199 HSP 1 e-value: 1.0E-65 bit: 250.0 Len: 126 Query Start:6 Query End:131 Subject Strand: NEGATIVE Subject Start: 1 Subject End: 126 .....agcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacag.. .....agcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacag.. HSP 2 e-value: 6.0E-24 bit: 111.0 Len: 56 Query Start:6 Query End:61 Subject Strand: NEGATIVE Subject Start: 227 Subject End: 282 .....agcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcaga........................................................................ .....agcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcaga........................................................................ Subject: smit_SMT_1926_SMT_1927|mevalonate kinase:cell division protein FtsH|POSITIVE:NEGATIVE|[1868123,1868497]|375 HSP 1 e-value: 1.0E-65 bit: 250.0 Len: 126 Query Start:6 Query End:131 Subject Strand: POSITIVE Subject Start: 250 Subject End: 375 .....agcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacag.. .....agcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacag.. HSP 2 e-value: 4.0E-59 bit: 228.0 Len: 115 Query Start:6 Query End:120 Subject Strand: NEGATIVE Subject Start: 1 Subject End: 115 .....agcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctaga............. .....agcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctaga............. Subject: smit_SMT_1884_SMT_1885|hypothetical protein:hypothetical protein predicted by Glimmer/Critica|POSITIVE:NEGATIVE|[1839299,1839919]|621 HSP 1 e-value: 1.0E-65 bit: 250.0 Len: 126 Query Start:6 Query End:131 Subject Strand: POSITIVE Subject Start: 496 Subject End: 621 .....agcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacag.. .....agcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacag.. Subject: smit_SMT_1873_SMT_1874|K02006 cobalt transport system ATP-binding protein:hypothetical protein|POSITIVE:NEGATIVE|[1832412,1832712]|301 HSP 1 e-value: 1.0E-65 bit: 250.0 Len: 126 Query Start:6 Query End:131 Subject Strand: NEGATIVE Subject Start: 1 Subject End: 126 .....agcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacag.. .....agcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacag.. Subject: smit_SMT_2001_SMT_2002|membrane protease protein family:hypothetical protein predicted by Glimmer/Critica|POSITIVE:NEGATIVE|[1923950,1924234]|285 HSP 1 e-value: 2.0E-63 bit: 242.0 Len: 126 Query Start:6 Query End:131 Subject Strand: POSITIVE Subject Start: 160 Subject End: 285 .....agcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacag.. .....agcgcccaatacgcaacccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacag.. Subject: smit_SMT_1845_SMT_1846|K01191 alpha-mannosidase:acyl-ACP thioesterase, putative|POSITIVE:POSITIVE|[1810496,1811549]|1054 HSP 1 e-value: 2.0E-63 bit: 242.0 Len: 128 Query Start:6 Query End:133 Subject Strand: POSITIVE Subject Start: 582 Subject End: 709 .....agcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacagct .....agcgcccaatacgcaarscgcctmtccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacagct Subject: smit_SMT_1955_SMT_1956|carbamoyl-phosphate synthase small chain:hypothetical protein predicted by Glimmer/Critica|NEGATIVE:NEGATIVE|[1889499,1889626]|128 HSP 1 e-value: 1.0E-61 bit: 236.0 Len: 123 Query Start:11 Query End:133 Subject Strand: NEGATIVE Subject Start: 1 Subject End: 123 ..........ccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacagct ..........ccaatacgcaaaccgcttctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacagct Subject: smit_SMT_1558_SMT_1559|hypothetical protein:6- phosphofructokinase|NEGATIVE:NEGATIVE|[1544149, 1544703]|555 HSP 1 e-value: 6.0E-61 bit: 234.0 Len: 128 Query Start:6 Query End:133 Subject Strand: NEGATIVE Subject Start: 191 Subject End: 317 .....agcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacagct .....agcgcccaatacgcaa-ccgcctctccccgcgcgttggccgattcattaatgcrgagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacagct Subject: smit_SMT_2024_SMT_2025|hypothetical protein:hypothetical protein predicted by Glimmer/Critica|POSITIVE:NEGATIVE|[1940751,1941084]|334 HSP 1 e-value: 4.0E-59 bit: 228.0 Len: 115 Query Start:6 Query End:120 Subject Strand: NEGATIVE Subject Start: 1 Subject End: 115 .....agcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctaga............. .....agcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctaga............. Subject: smit_SMT_1981_SMT_1982|K03696 ATP-dependent Clp protease ATP-binding subunit ClpC:conserved hypothetical protein|POSITIVE:POSITIVE|[1912929,1913175]|247 HSP 1 e-value: 4.0E-59 bit: 228.0 Len: 115 Query Start:6 Query End:120 Subject Strand: NEGATIVE Subject Start: 1 Subject End: 115 .....agcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctaga............. .....agcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctaga............. Subject: smit_SMT_1942_SMT_1943|zinc metalloprotease ZmpB, truncated:alpha/beta hydrolase fold|POSITIVE:NEGATIVE|[1878445,1878659]|215 HSP 1 e-value: 4.0E-59 bit: 228.0 Len: 115 Query Start:6 Query End:120 Subject Strand: NEGATIVE Subject Start: 1 Subject End: 115 .....agcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctaga............. .....agcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctaga............. Subject: smit_SMT_1887_SMT_1888|LicC protein:K02026 multiple sugar transport system permease protein|POSITIVE:POSITIVE|[1841801,1842227]|427 HSP 1 e-value: 4.0E-59 bit: 228.0 Len: 115 Query Start:6 Query End:120 Subject Strand: NEGATIVE Subject Start: 1 Subject End: 115 .....agcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctaga............. .....agcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctaga............. Subject: smit_SMT_1851_SMT_1852|ABC transporter, permease protein, putative:1,4-alpha-glucan branching enzyme|NEGATIVE:NEGATIVE|[1815812,1816556]|745 HSP 1 e-value: 3.0E-56 bit: 218.0 Len: 110 Query Start:6 Query End:115 Subject Strand: POSITIVE Subject Start: 636 Subject End: 745 .....agcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac.................. .....agcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac.................. Subject: smit_SMT_325_SMT_326|K02029 polar amino acid transport system permease protein:K02029 polar amino acid transport system permease protein|NEGATIVE:NEGATIVE|[263114,263306]|193 HSP 1 e-value: 3.0E-56 bit: 218.0 Len: 110 Query Start:6 Query End:115 Subject Strand: POSITIVE Subject Start: 84 Subject End: 193 .....agcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac.................. .....agcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac.................. Subject: smit_SMT_2044_SMT_2045|transcriptional activator TipA, putative:K+ transport system, NAD-binding component|NEGATIVE:POSITIVE|[1955976,1956278]|303 HSP 1 e-value: 5.0E-55 bit: 214.0 Len: 124 Query Start:10 Query End:133 Subject Strand: NEGATIVE Subject Start: 80 Subject End: 203 .........cccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacagct .........cccaatacgcaaaccgcctctccccgcgcgtgggcgatttcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacagct Subject: smit_SMT_1971_SMT_1972|hypothetical protein predicted by Glimmer/Critica:uncharacterized conserved phage related protein|POSITIVE:POSITIVE|[1903587,1903801]|215 HSP 1 e-value: 8.0E-54 bit: 210.0 Len: 110 Query Start:6 Query End:115 Subject Strand: NEGATIVE Subject Start: 1 Subject End: 110 .....agcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac.................. .....agcgcccaatacgcaaaccgcttctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcac.................. Subject: smit_SMT_303_SMT_304|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[245819,246719]|901 HSP 1 e-value: 2.0E-51 bit: 202.0 Len: 102 Query Start:6 Query End:107 Subject Strand: POSITIVE Subject Start: 800 Subject End: 901 .....agcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctagg.......................... .....agcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctagg.......................... HSP 2 e-value: 2.0E-48 bit: 192.0 Len: 101 Query Start:33 Query End:133 Subject Strand: NEGATIVE Subject Start: 119 Subject End: 219 ................................ccgcgcgttggccgattcattaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacagct ................................ccgcgcgttggccgattcataaatgcagagcttgcatgcctgcaggtcgactctagaggatccccctgtgctaggatggtcacctagatcaggaaacagct Subject: smit_SMT_2005_SMT_2006|pullulanase, type I:hypothetical protein predicted by Glimmer/Critica|NEGATIVE:POSITIVE|[1926380,1926611]|232 HSP 1 e-value: 2.0E-17 bit: 89.7 Len: 45 Query Start:89 Query End:133 Subject Strand: POSITIVE Subject Start: 109 Subject End: 153 ........................................................................................aggatccccctgtgctaggatggtcacctagatcaggaaacagct ........................................................................................aggatccccctgtgctaggatggtcacctagatcaggaaacagct HSP 2 e-value: 8.0E-11 bit: 67.9 Len: 34 Query Start:6 Query End:39 Subject Strand: NEGATIVE Subject Start: 1 Subject End: 34 .....agcgcccaatacgcaaaccgcctctccccgcgcg.............................................................................................. .....agcgcccaatacgcaaaccgcctctccccgcgcg.............................................................................................. Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGCGCCCAAUACGCAAACCGCCUCUCCCCGCGCGUUGGCCGAUUCAUUAAUGCAGAGCUUGCAUGCCUGCAGGUCGACUCUAGAGGAUCCCCCUGUGCUAGGAUGGUCACCUAGAUCAGGAAACAGCU ..((.((((..(((...............)))..)))).)).........(((((.((......)))))))....((.(((((..((((..((((...))))..))))..))))))).(....).... (-36.76) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGCUGUUUCCUGAUCUAGGUGACCAUCCUAGCACAGGGGGAUCCUCUAGAGUCGACCUGCAGGCAUGCAAGCUCUGCAUUAAUGAAUCGGCCAACGCGCGGGGAGAGGCGGUUUGCGUAUUGGGCGCU .(((.(((((((.((((((.((((.((((.....)))))).)).))))))(((((..(((((((......)).)))))........))))).......))))))).))).....((((.....)))). (-40.30) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 1112 50 1 191 142 smit:SMT_1986|5end_mercuric_reductase_1914843..1915073_POSITIVE 1112 50 1 224 175 smit:SMT_325|5end_K02029_polar_amino_acid_transport_system_permease_protein_263023..263253_NEGATIVE 1033 110 61 198 149 smit:SMT_959|5end_1-acyl-sn-glycerol-3-phosphate_acyltransferase_905283..905513_POSITIVE 912 86 41 231 186 smit:SMT_1914|5end_K03696_ATP-dependent_Clp_protease_ATP-binding_subunit_ClpC_1859486..1859716_POSITIVE 775 44 5 230 192 smit:SMT_2045|5end_K+_transport_system,_NAD-binding_component_1956139..1956369_POSITIVE 741 35 1 231 197 smit:SMT_1972|5end_uncharacterized_conserved_phage_related_protein_1903662..1903892_POSITIVE 654 115 84 231 200 smit:SMT_2005|5end_pullulanase,_type_I_1926289..1926519_NEGATIVE 397 120 71 153 101 smit:SMT_1291|5end_hypothetical_protein_1253875..1254105_POSITIVE 378 77 32 180 131 smit:SMT_110|5end_alpha-L-fucosidase_57445..57675_POSITIVE 377 20 1 231 212 smit:SMT_1691|5end_putative_deoxyribonuclease_1674978..1675208_POSITIVE 375 48 2 214 159 smit:SMT_1626|5end_undecaprenyl-diphosphatase_1616028..1616258_NEGATIVE 358 80 31 150 89 smit:SMT_941|5end_hypothetical_protein_893558..893788_NEGATIVE 351 110 63 192 130 smit:SMT_849|5end_hypothetical_protein_796127..796357_NEGATIVE 348 70 21 157 94 smit:SMT_1647|5end_3-isopropylmalate_dehydratase_small_subunit_1633403..1633633_POSITIVE 340 70 21 127 70 smit:SMT_1915|5end_pyridoxine_biosynthesis_protein_1860329..1860559_POSITIVE 333 70 30 70 30 smit:SMT_1634|5end_2,3,4,5-tetrahydropyridine-2-carboxylate_N-succinyltransferase,_putative_1621074..1621304_POSITIVE 333 107 63 65 17 smit:SMT_1602|5end_hypothetical_protein_1592385..1592615_POSITIVE 320 41 3 74 23 smit:SMT_2052|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1961591..1961821_NEGATIVE 320 80 32 165 118 smit:SMT_219|5end_phosphatidate_cytidylyltransferase_150922..151152_POSITIVE 316 59 14 225 169 smit:SMT_1645|5end_hypothetical_protein_1631738..1631968_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 1111 110 61 85 36 smit:SMT_2024|3end_hypothetical_protein_1940690..1940840_POSITIVE 1111 110 61 74 25 smit:SMT_2002|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1924145..1924295_NEGATIVE 1111 110 61 85 36 smit:SMT_1981|3end_K03696_ATP-dependent_Clp_protease_ATP-binding_subunit_ClpC_1912868..1913018_POSITIVE 1111 110 61 90 41 smit:SMT_1971|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1903526..1903676_POSITIVE 1111 110 61 74 25 smit:SMT_1966|3end_DNA_polymerase_III_alpha_subunit_1898328..1898478_POSITIVE 1111 110 61 85 36 smit:SMT_1942|3end_zinc_metalloprotease_ZmpB,_truncated_1878384..1878534_POSITIVE 1111 110 61 74 25 smit:SMT_1927|3end_cell_division_protein_FtsH_1868408..1868558_NEGATIVE 1111 110 61 85 36 smit:SMT_1926|3end_mevalonate_kinase_1868062..1868212_POSITIVE 1111 110 61 85 36 smit:SMT_1887|3end_LicC_protein_1841740..1841890_POSITIVE 1111 110 61 74 25 smit:SMT_1885|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1839830..1839980_NEGATIVE 1111 110 61 74 25 smit:SMT_1873|3end_K02006_cobalt_transport_system_ATP-binding_protein_1832351..1832501_POSITIVE 1111 70 21 50 1 smit:SMT_1852|3end_1,4-alpha-glucan_branching_enzyme_1816467..1816617_NEGATIVE 1111 70 21 50 1 smit:SMT_326|3end_K02029_polar_amino_acid_transport_system_permease_protein_263217..263367_NEGATIVE 1033 110 61 74 25 smit:SMT_958|3end_hypothetical_protein_predicted_by_Glimmer/Critica_905239..905389_POSITIVE 632 120 90 31 1 smit:SMT_479|3end_K03308_neurotransmitter:Na+_symporter,_NSS_family_415604..415754_NEGATIVE 504 120 94 27 1 smit:SMT_2030|3end_chromosomal_replication_initiator_protein_DnaA_1944790..1944940_NEGATIVE 410 120 98 23 1 smit:SMT_1895|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1847402..1847552_POSITIVE 352 46 2 151 98 smit:SMT_1627|3end_hypothetical_protein_1616089..1616239_NEGATIVE 350 39 1 66 24 smit:SMT_1941|3end_hypothetical_protein_1877663..1877813_POSITIVE 348 70 21 147 84 smit:SMT_1646|3end_3-isopropylmalate_dehydratase_large_subunit_1633473..1633623_POSITIVE