Origin IGS: nnnnntgtctctggtcgtggagacaaggacgtagctgcgattgcagactacctagaagctaaaaaataaaagatggaaaaattacagtcttttctctcacagagagcttgtggttgctggaaacaagcagagaaaactgtaaggttgggtccatctgaaacaagagaaaaaaacataattctcaagggagtgccctttatcgcacagcctgttacaggaaatatctgagacaggaatgagagataggagaaatcctataattgaggtggcaccgcgaatttcgtcctcacgcaagttattttgcgtggggatttttatatgtctattataggttcagctgtttagttttagattattgttcattattaaatagattgctacaaaaaatttgcgaacgaagtgagcttttaaaagatatttcccgccatagtggtatcctatagaagcaaatatgcttctataggacggaatttttgagagtaaaatagttcggggaactattttagcctgagcctagaaatgaatgagcgaggagcttaaaaattaagtctttcatttaatggatttctaaaatcatccactggataattttacctcccgtccgcactatttaagggaaatagaaaaagataaaaaattaaaaataaggaactgaaagggaaattaca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tgtaatttccctttcagttccttatttttaattttttatctttttctatttcccttaaatagtgcggacgggaggtaaaattatccagtggatgattttagaaatccattaaatgaaagacttaatttttaagctcctcgctcattcatttctaggctcaggctaaaatagttccccgaactattttactctcaaaaattccgtcctatagaagcatatttgcttctataggataccactatggcgggaaatatcttttaaaagctcacttcgttcgcaaattttttgtagcaatctatttaataatgaacaataatctaaaactaaacagctgaacctataatagacatataaaaatccccacgcaaaataacttgcgtgaggacgaaattcgcggtgccacctcaattataggatttctcctatctctcattcctgtctcagatatttcctgtaacaggctgtgcgataaagggcactcccttgagaattatgtttttttctcttgtttcagatggacccaaccttacagttttctctgcttgtttccagcaaccacaagctctctgtgagagaaaagactgtaatttttccatcttttattttttagcttctaggtagtctgcaatcgcagctacgtccttgtctccacgaccagagacannnnn Mask Tandem Repeat Region ================================================ nnnnntgtctctggtcgtggagacaaggacgtagctgcgattgcagactacctagaagctaaaaaataaaagatggaaaaattacagtcttttctctcacagagagcttgtggttgctggaaacaagcagagaaaactgtaaggttgggtccatctgaaacaagagaaaaaaacataattctcaagggagtgccctttatcgcacagcctgttacaggaaatatctgagacaggaatgagagataggagaaatcctataattgaggtggcaccgcgaatttcgtcctcacgcaagttattttgcgtggggatttttatatgtctattataggttcagctgtttagttttagattattgttcattattaaatagattgctacaaaaaatttgcgaacgaagtgagcttttaaaagatatttcccgccatagtggtatcctatagaagcaaatatgcttctataggacggaatttttgagagtaaaatagttcggggaactattttagcctgagcctagaaatgaatgagcgaggagcttaaaaattaagtctttcatttaatggatttctaaaatcatccactggataattttacctcccgtccgcactatttaagggaaatagaaaaagataaaaaattaaaaataaggaactgaaagggaaattaca Find is-nt database================================================ Query_seq: SMT_362:SMT_363|SMT_362:SMT_363:K02519 translation initiation factor IF-2:K02519 translation initiation factor IF-2:->->:298011..298678 668 nnnnntgtctctggtcgtggagacaaggacgtagctgcgattgcagactacctagaagctaaaaaataaaagatggaaaaattacagtcttttctctcacagagagcttgtggttgctggaaacaagcagagaaaactgtaaggttgggtccatctgaaacaagagaaaaaaacataattctcaagggagtgccctttatcgcacagcctgttacaggaaatatctgagacaggaatgagagataggagaaatcctataattgaggtggcaccgcgaatttcgtcctcacgcaagttattttgcgtggggatttttatatgtctattataggttcagctgtttagttttagattattgttcattattaaatagattgctacaaaaaatttgcgaacgaagtgagcttttaaaagatatttcccgccatagtggtatcctatagaagcaaatatgcttctataggacggaatttttgagagtaaaatagttcggggaactattttagcctgagcctagaaatgaatgagcgaggagcttaaaaattaagtctttcatttaatggatttctaaaatcatccactggataattttacctcccgtccgcactatttaagggaaatagaaaaagataaaaaattaaaaataaggaactgaaagggaaattaca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_362:SMT_363|SMT_362:SMT_363:K02519 translation initiation factor IF-2:K02519 translation initiation factor IF-2:->->:298011..298678 668 nnnnntgtctctggtcgtggagacaaggacgtagctgcgattgcagactacctagaagctaaaaaataaaagatggaaaaattacagtcttttctctcacagagagcttgtggttgctggaaacaagcagagaaaactgtaaggttgggtccatctgaaacaagagaaaaaaacataattctcaagggagtgccctttatcgcacagcctgttacaggaaatatctgagacaggaatgagagataggagaaatcctataattgaggtggcaccgcgaatttcgtcctcacgcaagttattttgcgtggggatttttatatgtctattataggttcagctgtttagttttagattattgttcattattaaatagattgctacaaaaaatttgcgaacgaagtgagcttttaaaagatatttcccgccatagtggtatcctatagaagcaaatatgcttctataggacggaatttttgagagtaaaatagttcggggaactattttagcctgagcctagaaatgaatgagcgaggagcttaaaaattaagtctttcatttaatggatttctaaaatcatccactggataattttacctcccgtccgcactatttaagggaaatagaaaaagataaaaaattaaaaataaggaactgaaagggaaattaca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_362:SMT_363|SMT_362:SMT_363:K02519 translation initiation factor IF-2:K02519 translation initiation factor IF-2:->->:298011..298678 668 nnnnntgtctctggtcgtggagacaaggacgtagctgcgattgcagactacctagaagctaaaaaataaaagatggaaaaattacagtcttttctctcacagagagcttgtggttgctggaaacaagcagagaaaactgtaaggttgggtccatctgaaacaagagaaaaaaacataattctcaagggagtgccctttatcgcacagcctgttacaggaaatatctgagacaggaatgagagataggagaaatcctataattgaggtggcaccgcgaatttcgtcctcacgcaagttattttgcgtggggatttttatatgtctattataggttcagctgtttagttttagattattgttcattattaaatagattgctacaaaaaatttgcgaacgaagtgagcttttaaaagatatttcccgccatagtggtatcctatagaagcaaatatgcttctataggacggaatttttgagagtaaaatagttcggggaactattttagcctgagcctagaaatgaatgagcgaggagcttaaaaattaagtctttcatttaatggatttctaaaatcatccactggataattttacctcccgtccgcactatttaagggaaatagaaaaagataaaaaattaaaaataaggaactgaaagggaaattaca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 2 Min: 54 Max: 225 Len: 172 Subject: UniRef90_Q04IY3 Cluster: Hypothetical protein; n=2; Streptococcus pneumoniae|Rep: Hypothetical protein - Streptococcus pneumoniae serotype 2 (strain D39 / NCTC 7466) HSP 1 e-value: 2.0E-18 bit: 95.1 Len: 153 Query Start:73 Query End:225 Subject Strand: null Subject Start: 1 Subject End: 51 ........................................................................ M E K L Q S F L S Q R A C G C W K Q A E K T V R L G P S E T R E K N I I L K G V P F I A Q P V T G N I ........................................................................................................................................................................................................................................................................................................................................................................................................................................................... ........................................................................ M E K L Q F F L S Q R A C G C W K Q A E K A V K L G S S E T R E E N I I L K G V P F I A Q P V T G D I ........................................................................................................................................................................................................................................................................................................................................................................................................................................................... Subject: UniRef90_Q97P26 Cluster: Hypothetical protein; n=1; Streptococcus pneumoniae|Rep: Hypothetical protein - Streptococcus pneumoniae HSP 1 e-value: 6.0E-14 bit: 80.1 Len: 132 Query Start:54 Query End:185 Subject Strand: null Subject Start: 1 Subject End: 44 ..................................................... L R I M F F S L V S D G P N L T V F S A C F Q Q P Q A L C E R K D C N F S I F Y F L A S ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... ..................................................... M R M M F S S L V S D E P N F T A F S A C F Q Q P Q A L C E R K N C N F S I Y Y F L A S ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... nnnnntgtctctggtcgtggagacaaggacgtagctgcgattgcagactacctnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntgagacaggaatgagagataggagaaatcctataattgaggtggcaccgcgaatttcgtcctcacgcaagttattttgcgtggggatttttatatgtctattataggttcagctgtttagttttagattattgttcattattaaatagattgctacaaaaaatttgcgaacgaagtgagcttttaaaagatatttcccgccatagtggtatcctatagaagcaaatatgcttctataggacggaatttttgagagtaaaatagttcggggaactattttagcctgagcctagaaatgaatgagcgaggagcttaaaaattaagtctttcatttaatggatttctaaaatcatccactggataattttacctcccgtccgcactatttaagggaaatagaaaaagataaaaaattaaaaataaggaactgaaagggaaattaca Predict ORF larger than 30AA ================================================ Protein_Len: 30 Strand: + Start: 66 End: 155 ................................................................. M K D G K I T V F S L T E S L W L L E T S R E N C K V G S I ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 51 Strand: + Start: 73 End: 225 ........................................................................ M E K L Q S F L S Q R A C G C W K Q A E K T V R L G P S E T R E K N I I L K G V P F I A Q P V T G N I ........................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 40 Strand: + Start: 209 End: 328 ................................................................................................................................................................................................................ M L Q E I S E T G M R D R R N P I I E V A P R I S S S R K L F C V G I F I C L L .................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: + Start: 261 End: 440 .................................................................................................................................................................................................................................................................... M R W H R E F R P H A S Y F A W G F L Y V Y Y R F S C L V L D Y C S L L N R L L Q K I C E R S E L L K D I S R H S G I L .................................................................................................................................................................................................................................... Protein_Len: 32 Strand: - Start: 356 End: 451 ................................................................................................................................................................................................................................................................................................................................................................... Q E N N F L N S C F I Q S R L S S K F S I E R W L P I R Y F C M ......................................................................................................................................................................................................................... Protein_Len: 55 Strand: - Start: 337 End: 501 ................................................................................................................................................................................................................................................................................................................................................ S N L K L N N N M I L Y I A V F F K R V F H A K L L Y K G G Y H Y G I S A F I S R Y S P I K S L L I T R P V M ....................................................................................................................................................................... Protein_Len: 60 Strand: - Start: 100 End: 318 ................................................................................................... Y P Q T W R F C S F F V Y N E L S H G K D C L R N C S I D S V P I L S L L F G I I S T A G R I E D E R L N N Q T P I K M .............................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 34 Strand: - Start: 62 End: 163 ............................................................. F I F S P F I V T K E R V S L K H N S S V L L S F Q L T P D M Q F M ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: - Start: 51 End: 233 .................................................. S A L F Y F I S F N C D K R E C L A Q P Q Q F C A S F V T L N P G D S V L S F F M I R L P T G K I A C G T V P F I Q S M ................................................................................................................................................................................................................................................................................................................................................................................................................................................... nnnnntgtctctggtcgtggagacaaggacgtagctgcgattgcagactacctnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntgagacaggaatgagagataggagaaatcctataattgaggtggcaccgcgaatttcgtcctcacgcaagttattttgcgtggggatttttatatgtctattataggttcagctgtttagttttagattattgttcattattaaatagattgctacaaaaaatttgcgaacgaagtgagcttttaaaagatatttcccgccatagtggtatcctatagaagcaaatatgcttctataggacggaatttttgagagtaaaatagttcggggaactattttagcctgagcctagaaatgaatgagcgaggagcttaaaaattaagtctttcatttaatggatttctaaaatcatccactggataattttacctcccgtccgcactatttaagggaaatagaaaaagataaaaaattaaaaataaggaactgaaagggaaattaca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ nnnnntgtctctggtcgtggagacaaggacgtagctgcgattgcagactacctnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntgagacaggaatgagagataggagaaatcctataattgaggtggcaccgcgaatttcgtcctcacgcaagttattttgcgtggggatttttatatgtctattataggttcagctgtttagttttagattattgttcattattaaatagattgctacaaaaaatttgcgaacgaagtgagcttttaaaagatatttcccgccatagtggtatcctatagaagcaaatatgcttctataggacggaatttttgagagtaaaatagttcggggaactattttagcctgagcctagaaatgaatgagcgaggagcttaaaaattaagtctttcatttaatggatttctaaaatcatccactggataattttacctcccgtccgcactatttaagggaaatagaaaaagataaaaaattaaaaataaggaactgaaagggaaattaca Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 100 HP_score: -12.8 Tail_Score: -5.08178 Start: 284 End: 311 Full_Region: GCACCGCGAATTTCG TCCTCACGCAAG TTAT TTTGCGTGGGGA TTTTTATATGTCTAT ...........................................................................................................................................................................................................................................................................................TCCTCACGCAAGTTATTTTGCGTGGGGA..................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: + Conf: 74 HP_score: -5.8 Tail_Score: -4.59773 Start: 431 End: 470 Full_Region: TATTTCCCGCCATAG TGGTATCCTATAGAAGCA AATA TGCTTCTATAGGACGGAA TTTTTGAGAGTAAAA ..............................................................................................................................................................................................................................................................................................................................................................................................................................................TGGTATCCTATAGAAGCAAATATGCTTCTATAGGACGGAA...................................................................................................................................................................................................... TransTerm Strand: + Conf: 73 HP_score: -7.2 Tail_Score: -4.13945 Start: 486 End: 500 Full_Region: TTTTTGAGAGTAAAA TAGTTC GGG GAACTA TTTTAGCCTGAGCCT .....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TAGTTCGGGGAACTA........................................................................................................................................................................ TransTerm Strand: - Conf: 76 HP_score: -7.2 Tail_Score: -4.34402 Start: 486 End: 500 Full_Region: aggctcaggctaaaa tagttc ccc gaacta ttttactctcaaaaa .....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tagttccccgaacta........................................................................................................................................................................ Find igs database================================================ Query_seq: SMT_362:SMT_363|SMT_362:SMT_363:K02519 translation initiation factor IF-2:K02519 translation initiation factor IF-2:->->:298011..298678 668 nnnnntgtctctggtcgtggagacaaggacgtagctgcgattgcagactacctnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntgagacaggaatgagagataggagaaatcctataattgaggtggcaccgcgaatttcgtcctcacgcaagttattttgcgtggggatttttatatgtctattataggttcagctgtttagttttagattattgttcattattaaatagattgctacaaaaaatttgcgaacgaagtgagcttttaaaagatatttcccgccatagtggtatcctatagaagcaaatatgcttctataggacggaatttttgagagtaaaatagttcggggaactattttagcctgagcctagaaatgaatgagcgaggagcttaaaaattaagtctttcatttaatggatttctaaaatcatccactggataattttacctcccgtccgcactatttaagggaaatagaaaaagataaaaaattaaaaataaggaactgaaagggaaattaca Intra-Species Hit: Count: 2 Min: 6 Max: 623 Len: 618 Subject: smit_SMT_362_SMT_363|K02519 translation initiation factor IF-2:K02519 translation initiation factor IF-2|POSITIVE:POSITIVE|[298011,298678]|668 HSP 1 e-value: 0.0 bit: 789.0 Len: 398 Query Start:226 Query End:623 Subject Strand: POSITIVE Subject Start: 226 Subject End: 623 .................................................................................................................................................................................................................................tgagacaggaatgagagataggagaaatcctataattgaggtggcaccgcgaatttcgtcctcacgcaagttattttgcgtggggatttttatatgtctattataggttcagctgtttagttttagattattgttcattattaaatagattgctacaaaaaatttgcgaacgaagtgagcttttaaaagatatttcccgccatagtggtatcctatagaagcaaatatgcttctataggacggaatttttgagagtaaaatagttcggggaactattttagcctgagcctagaaatgaatgagcgaggagcttaaaaattaagtctttcatttaatggatttctaaaatcatccactggataattttacctcccgtccgcactatttaagggaaatag............................................. .................................................................................................................................................................................................................................tgagacaggaatgagagataggagaaatcctataattgaggtggcaccgcgaatttcgtcctcacgcaagttattttgcgtggggatttttatatgtctattataggttcagctgtttagttttagattattgttcattattaaatagattgctacaaaaaatttgcgaacgaagtgagcttttaaaagatatttcccgccatagtggtatcctatagaagcaaatatgcttctataggacggaatttttgagagtaaaatagttcggggaactattttagcctgagcctagaaatgaatgagcgaggagcttaaaaattaagtctttcatttaatggatttctaaaatcatccactggataattttacctcccgtccgcactatttaagggaaatag............................................. HSP 2 e-value: 2.0E-18 bit: 95.6 Len: 48 Query Start:6 Query End:53 Subject Strand: POSITIVE Subject Start: 6 Subject End: 53 .....tgtctctggtcgtggagacaaggacgtagctgcgattgcagactacct....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... .....tgtctctggtcgtggagacaaggacgtagctgcgattgcagactacct....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Subject: smit_SMT_1958_SMT_1959|tryptophan synthase, beta subunit:hypothetical protein predicted by Glimmer/Critica|POSITIVE:POSITIVE|[1892113,1892724]|612 HSP 1 e-value: 1.0E-133 bit: 478.0 Len: 241 Query Start:226 Query End:466 Subject Strand: POSITIVE Subject Start: 157 Subject End: 397 .................................................................................................................................................................................................................................tgagacaggaatgagagataggagaaatcctataattgaggtggcaccgcgaatttcgtcctcacgcaagttattttgcgtggggatttttatatgtctattataggttcagctgtttagttttagattattgttcattattaaatagattgctacaaaaaatttgcgaacgaagtgagcttttaaaagatatttcccgccatagtggtatcctatagaagcaaatatgcttctataggac.......................................................................................................................................................................................................... .................................................................................................................................................................................................................................tgagacaggaatgagagataggagaaatcctataattgaggtggcaccgcgaatttcgtcctcacgcaagttattttgcgtggggatttttatatgtctattataggttcagctgtttagttttagattattgttcattattaaatagattgctacaaaaaatttgcgaacgaagtgagcttttaaaagatatttcccgccatagtggtatcctatagaagcaaatatgcttctataggac.......................................................................................................................................................................................................... Inter-species Hit: Count: 3 Min: 464 Max: 622 Len: 159 Subject: NC_008533_SPD_1626_SPD_1628|exodeoxyribonuclease III:xanthine phosphoribosyltransferase|POSITIVE:POSITIVE|[1641580, 1642850]|1271 HSP 1 e-value: 4.0E-32 bit: 141.0 Len: 159 Query Start:464 Query End:622 Subject Strand: NEGATIVE Subject Start: 946 Subject End: 1104 ...............................................................................................................................................................................................................................................................................................................................................................................................................................................................................gacggaatttttgagagtaaaatagttcggggaactattttagcctgagcctagaaatgaatgagcgaggagcttaaaaattaagtctttcatttaatggatttctaaaatcatccactggataattttacctcccgtccgcactatttaagggaaata.............................................. ...............................................................................................................................................................................................................................................................................................................................................................................................................................................................................gacgggatttttgagagtaaaatagttcggggaactattttagcctaagcctagaaatgaaagagctaggggctcaaaaattagggatgaaattccctggattcctgaaattattcacaggataatttcacctcccgtccgcactaattaagggaaata.............................................. Subject: NC_003098_spr1661_spr1662|hypothetical protein:xanthine phosphoribosyltransferase|POSITIVE:POSITIVE|[1634443, 1635319]|877 HSP 1 e-value: 4.0E-32 bit: 141.0 Len: 159 Query Start:464 Query End:622 Subject Strand: NEGATIVE Subject Start: 552 Subject End: 710 ...............................................................................................................................................................................................................................................................................................................................................................................................................................................................................gacggaatttttgagagtaaaatagttcggggaactattttagcctgagcctagaaatgaatgagcgaggagcttaaaaattaagtctttcatttaatggatttctaaaatcatccactggataattttacctcccgtccgcactatttaagggaaata.............................................. ...............................................................................................................................................................................................................................................................................................................................................................................................................................................................................gacgggatttttgagagtaaaatagttcggggaactattttagcctaagcctagaaatgaaagagctaggggctcaaaaattagggatgaaattccctggattcctgaaattattcacaggataatttcacctcccgtccgcactaattaagggaaata.............................................. Subject: NC_003028_SP_1845_SP_1847|exodeoxyribonuclease:xanthine phosphoribosyltransferase|POSITIVE:POSITIVE|[1754015, 1755276]|1262 HSP 1 e-value: 4.0E-32 bit: 141.0 Len: 159 Query Start:464 Query End:622 Subject Strand: NEGATIVE Subject Start: 937 Subject End: 1095 ...............................................................................................................................................................................................................................................................................................................................................................................................................................................................................gacggaatttttgagagtaaaatagttcggggaactattttagcctgagcctagaaatgaatgagcgaggagcttaaaaattaagtctttcatttaatggatttctaaaatcatccactggataattttacctcccgtccgcactatttaagggaaata.............................................. ...............................................................................................................................................................................................................................................................................................................................................................................................................................................................................gacgggatttttgagagtaaaatagttcggggaactattttagcctaagcctagaaatgaaagagctaggggctcaaaaattagggatgaaattccctggattcctgaaattattcacaggataatttcacctcccgtccgcactaattaagggaaata.............................................. Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GACGGAAUUUUUGAGAGUAAAAUAGUUCGGGGAACUAUUUUAGCCUGAGCCUAGAAAUGAAUGAGCGAGGAGCUUAAAAAUUAAGUCUUUCAUUUAAUGGAUUUCUAAAAUCAUCCACUGGAUAAUUUUACCUCCCGUCCGCACUAUUUAAGGGAAAUA (((((.......(((.(((((((.(((((((((...(((((((......((...((((((((((((.....)))))............)))))))...))....)))))))..))).)))))).))))))))))))))).................... (-39.11) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAUUUCCCUUAAAUAGUGCGGACGGGAGGUAAAAUUAUCCAGUGGAUGAUUUUAGAAAUCCAUUAAAUGAAAGACUUAAUUUUUAAGCUCCUCGCUCAUUCAUUUCUAGGCUCAGGCUAAAAUAGUUCCCCGAACUAUUUUACUCUCAAAAAUUCCGUC ......(((.((((((((.((.((((((.(((((((((((...))))))))))).............((((((......))))))..)))).)))))))).))))..)))...((..(((((((((((...)))))))))))..))............. (-32.50) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 462 70 21 162 113 smit:SMT_864|5end_K07485_transposase_814322..814552_POSITIVE 353 70 24 157 109 smit:SMT_1788|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1765029..1765259_NEGATIVE 353 70 24 157 109 smit:SMT_1197|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1158751..1158981_NEGATIVE 287 74 31 212 177 smit:SMT_832|5end_hypothetical_protein_775104..775334_NEGATIVE 265 69 25 55 13 smit:SMT_1754|5end_leader_peptidase_(prepilin_peptidase)_/_N-methyltransferase_1733022..1733252_NEGATIVE 257 76 33 121 78 smit:SMT_768|5end_hypothetical_protein_679243..679473_NEGATIVE 255 70 28 50 4 smit:SMT_1216|5end_hypothetical_protein_1174879..1175109_POSITIVE 251 69 25 55 13 smit:SMT_2028|5end_leader_peptidase_(prepilin_peptidase)_/_N-methyltransferase_1943729..1943959_NEGATIVE 250 104 61 170 127 smit:SMT_765|5end_hypothetical_protein_672620..672850_NEGATIVE 249 63 28 62 21 smit:SMT_1123|5end_hypothetical_protein_1059292..1059522_POSITIVE 245 148 115 159 123 smit:SMT_732|5end_K07467_phage_replication_initiation_protein_639409..639639_NEGATIVE 243 85 44 227 180 smit:SMT_2083|5end_K06147_ATP-binding_cassette,_subfamily_B,_bacterial_1982411..1982641_POSITIVE 243 66 21 73 35 smit:SMT_718|5end_NADP-specific_glutamate_dehydrogenase_623176..623406_POSITIVE 239 70 23 229 178 smit:SMT_2123|5end_hypothetical_protein_2008201..2008431_POSITIVE 239 60 15 173 130 smit:SMT_1218|5end_DNA_polymerase_I_1179357..1179587_NEGATIVE 238 145 114 178 151 smit:SMT_629|5end_glycogen_synthase_533967..534197_POSITIVE 238 150 116 124 89 smit:SMT_572|5end_fructose_repressor_490239..490469_POSITIVE 237 75 46 160 129 smit:SMT_1702|5end_penicillin-binding_protein_2B_1684592..1684822_POSITIVE 234 90 49 67 26 smit:SMT_2053|5end_hypothetical_membrane_associated_protein_1964422..1964652_NEGATIVE 234 66 21 79 40 smit:SMT_2019|5end_dTDP-glucose_4,6-dehydratase_1937054..1937284_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 353 70 24 71 23 smit:SMT_1198|3end_phosphoribosylaminoimidazole-succinocarboxamide_synthase_1158837..1158987_NEGATIVE 287 74 31 133 98 smit:SMT_833|3end_hypothetical_protein_predicted_by_Glimmer/Critica_775183..775333_NEGATIVE 256 74 42 147 119 smit:SMT_225|3end_pullulanase_159666..159816_NEGATIVE 255 139 99 105 66 smit:SMT_397|3end_NAD(+)_synthetase_327423..327573_POSITIVE 244 70 27 75 20 smit:SMT_939|3end_phosphomethylpyrimidine_kinase_891252..891402_NEGATIVE 244 59 18 107 69 smit:SMT_493|3end_K01996_branched-chain_amino_acid_transport_system_ATP-binding_protein_427927..428077_POSITIVE 243 142 116 129 108 smit:SMT_1301|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1263836..1263986_POSITIVE 238 87 46 138 106 smit:SMT_1070|3end_hypothetical_protein_1009644..1009794_NEGATIVE 237 75 48 80 55 smit:SMT_1020|3end_transcriptional_regulator,_TetR_family_956371..956521_POSITIVE 235 70 23 77 16 smit:SMT_2067|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1974216..1974366_POSITIVE 230 146 102 115 60 smit:SMT_1381|3end_gamma-glutamyl_phosphate_reductase_1358468..1358618_NEGATIVE 230 68 22 88 43 smit:SMT_914|3end_sucrose-6-phosphate_hydrolase_867673..867823_POSITIVE 230 79 40 40 4 smit:SMT_887|3end_ABC-type_polar_amino_acid_transport_system,_ATPase_component_835528..835678_POSITIVE 228 40 8 34 6 smit:SMT_2078|3end_acetyl_xylan_esterase,_putative_1979839..1979989_POSITIVE 228 50 1 126 60 smit:SMT_1140|3end_glycogen_phosphorylase_1081316..1081466_NEGATIVE 226 60 16 84 46 smit:SMT_1371|3end_hypothetical_protein_1350579..1350729_NEGATIVE 223 148 101 65 9 smit:SMT_1643|3end_2-isopropylmalate_synthase_1630772..1630922_POSITIVE 220 146 102 93 51 smit:SMT_766|3end_hypothetical_protein_673385..673535_NEGATIVE 218 136 93 139 97 smit:SMT_733|3end_hypothetical_protein_639412..639562_NEGATIVE 217 116 72 130 80 smit:SMT_859|3end_K02086_DNA_replication_protein_803381..803531_NEGATIVE