Origin IGS: gatgagtgtattggatgaagagtatctaaaaaatacacgaaaagtttataatgatttttgtaataaagctgatagttatgaatctgcaaaagattttatagataatattccagtagtgtatttggctaggtataaagcaattattttagctgagcatgaaagttgtgttaaaaatgatgaggcggtaaggaattttgttacttcagtattgttgtcagcgctagtatcagcactagtatcagcaaccatacaaaaaccagaatttataatatcgtttataattggcatggtttgggttgtctgtgttttccttctaatctattggaattttatagccaatactaagaagagacaaaaatatataaatgtttgtgtgcttattggatatttaaaatcaaaataaatttgatttaagttacttcactcatccacagaggttgcaatccagcctctgtttttcgataaaatagaggatgttgcgt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 acgcaacatcctctattttatcgaaaaacagaggctggattgcaacctctgtggatgagtgaagtaacttaaatcaaatttattttgattttaaatatccaataagcacacaaacatttatatatttttgtctcttcttagtattggctataaaattccaatagattagaaggaaaacacagacaacccaaaccatgccaattataaacgatattataaattctggtttttgtatggttgctgatactagtgctgatactagcgctgacaacaatactgaagtaacaaaattccttaccgcctcatcatttttaacacaactttcatgctcagctaaaataattgctttatacctagccaaatacactactggaatattatctataaaatcttttgcagattcataactatcagctttattacaaaaatcattataaacttttcgtgtattttttagatactcttcatccaatacactcatc Mask Tandem Repeat Region ================================================ gatgagtgtattggatgaagagtatctaaaaaatacacgaaaagtttataatgatttttgtaataaagctgatagttatgaatctgcaaaagattttatagataatattccagtagtgtatttggctaggtataaagcaattattttagctgagcatgaaagttgtgttaaaaatgatgaggcggtaaggaattttgttacttcagtattgttgnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnaccatacaaaaaccagaatttataatatcgtttataattggcatggtttgggttgtctgtgttttccttctaatctattggaattttatagccaatactaagaagagacaaaaatatataaatgtttgtgtgcttattggatatttaaaatcaaaataaatttgatttaagttacttcactcatccacagaggttgcaatccagcctctgtttttcgataaaatagaggatgttgcgt Find is-nt database================================================ Query_seq: SMT_340:SMT_341|SMT_340:SMT_341:chaperone protein:ThiJ/PfpI family protein:->->:280627..281108 482 gatgagtgtattggatgaagagtatctaaaaaatacacgaaaagtttataatgatttttgtaataaagctgatagttatgaatctgcaaaagattttatagataatattccagtagtgtatttggctaggtataaagcaattattttagctgagcatgaaagttgtgttaaaaatgatgaggcggtaaggaattttgttacttcagtattgttgtcagcgctagtatcagcactagtatcagcaaccatacaaaaaccagaatttataatatcgtttataattggcatggtttgggttgtctgtgttttccttctaatctattggaattttatagccaatactaagaagagacaaaaatatataaatgtttgtgtgcttattggatatttaaaatcaaaataaatttgatttaagttacttcactcatccacagaggttgcaatccagcctctgtttttcgataaaatagaggatgttgcgt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_340:SMT_341|SMT_340:SMT_341:chaperone protein:ThiJ/PfpI family protein:->->:280627..281108 482 gatgagtgtattggatgaagagtatctaaaaaatacacgaaaagtttataatgatttttgtaataaagctgatagttatgaatctgcaaaagattttatagataatattccagtagtgtatttggctaggtataaagcaattattttagctgagcatgaaagttgtgttaaaaatgatgaggcggtaaggaattttgttacttcagtattgttgtcagcgctagtatcagcactagtatcagcaaccatacaaaaaccagaatttataatatcgtttataattggcatggtttgggttgtctgtgttttccttctaatctattggaattttatagccaatactaagaagagacaaaaatatataaatgtttgtgtgcttattggatatttaaaatcaaaataaatttgatttaagttacttcactcatccacagaggttgcaatccagcctctgtttttcgataaaatagaggatgttgcgt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_340:SMT_341|SMT_340:SMT_341:chaperone protein:ThiJ/PfpI family protein:->->:280627..281108 482 gatgagtgtattggatgaagagtatctaaaaaatacacgaaaagtttataatgatttttgtaataaagctgatagttatgaatctgcaaaagattttatagataatattccagtagtgtatttggctaggtataaagcaattattttagctgagcatgaaagttgtgttaaaaatgatgaggcggtaaggaattttgttacttcagtattgttgtcagcgctagtatcagcactagtatcagcaaccatacaaaaaccagaatttataatatcgtttataattggcatggtttgggttgtctgtgttttccttctaatctattggaattttatagccaatactaagaagagacaaaaatatataaatgtttgtgtgcttattggatatttaaaatcaaaataaatttgatttaagttacttcactcatccacagaggttgcaatccagcctctgtttttcgataaaatagaggatgttgcgt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 1 Min: 2 Max: 142 Len: 141 Subject: UniRef90_Q97S72 Cluster: Hypothetical protein; n=1; Streptococcus pneumoniae|Rep: Hypothetical protein - Streptococcus pneumoniae HSP 1 e-value: 6.0E-16 bit: 85.5 Len: 141 Query Start:2 Query End:142 Subject Strand: null Subject Start: 1 Subject End: 47 . M S V L D E E Y L K N T R K V Y N D F C N K A D S Y E S A K D F I D N I P V V Y L A R Y K A I .................................................................................................................................................................................................................................................................................................................................................... . M S V L D E E Y L K N T R K V Y N D F C N Q A D N Y R T S K D F I D N I P I E Y L A R Y R E L .................................................................................................................................................................................................................................................................................................................................................... gnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnattttagctgagcatgaaagttgtgttaaaaatgatgaggcggtaaggaattttgttacttcagtattgttgtcagcgctagtatcagcactagtatcagcaaccatacaaaaaccagaatttataatatcgtttataattggcatggtttgggttgtctgtgttttccttctaatctattggaattttatagccaatactaagaagagacaaaaatatataaatgtttgtgtgcttattggatatttaaaatcaaaataaatttgatttaagttacttcactcatccacagaggttgcaatccagcctctgtttttcgataaaatagaggatgttgcgt Predict ORF larger than 30AA ================================================ Protein_Len: 45 Strand: + Start: 208 End: 342 ............................................................................................................................................................................................................... M V V S A S I S T S I S N H T K T R I Y N I V Y N W H G L G C L C F P S N L L E F Y S Q Y ............................................................................................................................................ Protein_Len: 60 Strand: + Start: 2 End: 400 . M S V L D E E Y L K N T R K V Y N D F C N K A D S Y E S A K D F I D N I P V V Y L A R Y K A I I L A E H E S C V K N D E .................................................................................. Protein_Len: 60 Strand: - Start: 79 End: 318 .............................................................................. I I K A S C S L Q T L F S S A T L F K T V E T N N D A S T D A S T D A V M C F G S N I I D N I I P M T Q T T Q T K R R M .................................................................................................................................................................... Protein_Len: 35 Strand: - Start: 29 End: 133 ............................ F I C S F N I I I K T I F S I T I F R C F I K Y I I N W Y H I Q S P M ............................................................................................................................................................................................................................................................................................................................................................. gnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnattttagctgagcatgaaagttgtgttaaaaatgatgaggcggtaaggaattttgttacttcagtattgttgtcagcgctagtatcagcactagtatcagcaaccatacaaaaaccagaatttataatatcgtttataattggcatggtttgggttgtctgtgttttccttctaatctattggaattttatagccaatactaagaagagacaaaaatatataaatgtttgtgtgcttattggatatttaaaatcaaaataaatttgatttaagttacttcactcatccacagaggttgcaatccagcctctgtttttcgataaaatagaggatgttgcgt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: - Score: 85 Start: 143 End: 172 ..............................................................................................................................................TTTAACACAACTTTCATGCTCAGCTAAAAT...................................................................................................................................................................................................................................................................................................................... PromScan Matrix: RpoD-17 Strand: - Score: 84 Start: 327 End: 356 ......................................................................................................................................................................................................................................................................................................................................TTTGTCTCTTCTTAGTATTGGCTATAAAAT.............................................................................................................................. Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ gnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnattttagctgagcatgaaagttgtgttaaaaatgatgaggcggtaaggaattttgttacttcagtattgttgtcagcgctagtatcagcactagtatcagcaaccatacaaaaaccagaatttataatatcgtttataattggcatggtttgggttgtctgtgttttccttctaatctattggaattttatagccaatactaagaagagacaaaaatatataaatgtttgtgtgcttattggatatttaaaatcaaaataaatttgatttaagttacttcactcatccacagaggttgcaatccagcctctgtttttcgataaaatagaggatgttgcgt Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 42 HP_score: -3.0 Tail_Score: -2.62668 Start: 390 End: 414 Full_Region: TGCTTATTGGATATT TAAAATCAAA ATAAA TTTGATTTAA GTTACTTCACTCATC .....................................................................................................................................................................................................................................................................................................................................................................................................TAAAATCAAAATAAATTTGATTTAA.................................................................... TransTerm Strand: + Conf: 50 HP_score: -2.9 Tail_Score: -3.81027 Start: 395 End: 409 Full_Region: ATTGGATATTTAAAA TCAAA ATAAA TTTGA TTTAAGTTACTTCAC ..........................................................................................................................................................................................................................................................................................................................................................................................................TCAAAATAAATTTGA......................................................................... TransTerm Strand: - Conf: 55 HP_score: -2.9 Tail_Score: -4.23758 Start: 395 End: 409 Full_Region: gtgaagtaacttaaa tcaaa tttat tttga ttttaaatatccaat ..........................................................................................................................................................................................................................................................................................................................................................................................................tcaaatttattttga......................................................................... TransTerm Strand: + Conf: 100 HP_score: -9.9 Tail_Score: -4.66609 Start: 432 End: 454 Full_Region: TACTTCACTCATCCA CAGAGGTTG CAATC CAGCCTCTG TTTTTCGATAAAATA ...............................................................................................................................................................................................................................................................................................................................................................................................................................................CAGAGGTTGCAATCCAGCCTCTG............................ Find igs database================================================ Query_seq: SMT_340:SMT_341|SMT_340:SMT_341:chaperone protein:ThiJ/PfpI family protein:->->:280627..281108 482 gnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnattttagctgagcatgaaagttgtgttaaaaatgatgaggcggtaaggaattttgttacttcagtattgttgtcagcgctagtatcagcactagtatcagcaaccatacaaaaaccagaatttataatatcgtttataattggcatggtttgggttgtctgtgttttccttctaatctattggaattttatagccaatactaagaagagacaaaaatatataaatgtttgtgtgcttattggatatttaaaatcaaaataaatttgatttaagttacttcactcatccacagaggttgcaatccagcctctgtttttcgataaaatagaggatgttgcgt Intra-Species Hit: Count: 1 Min: 143 Max: 482 Len: 340 Subject: smit_SMT_340_SMT_341|chaperone protein:ThiJ/PfpI family protein|POSITIVE:POSITIVE|[280627,281108]|482 HSP 1 e-value: 0.0 bit: 674.0 Len: 340 Query Start:143 Query End:482 Subject Strand: POSITIVE Subject Start: 143 Subject End: 482 ..............................................................................................................................................attttagctgagcatgaaagttgtgttaaaaatgatgaggcggtaaggaattttgttacttcagtattgttgtcagcgctagtatcagcactagtatcagcaaccatacaaaaaccagaatttataatatcgtttataattggcatggtttgggttgtctgtgttttccttctaatctattggaattttatagccaatactaagaagagacaaaaatatataaatgtttgtgtgcttattggatatttaaaatcaaaataaatttgatttaagttacttcactcatccacagaggttgcaatccagcctctgtttttcgataaaatagaggatgttgcgt ..............................................................................................................................................attttagctgagcatgaaagttgtgttaaaaatgatgaggcggtaaggaattttgttacttcagtattgttgtcagcgctagtatcagcactagtatcagcaaccatacaaaaaccagaatttataatatcgtttataattggcatggtttgggttgtctgtgttttccttctaatctattggaattttatagccaatactaagaagagacaaaaatatataaatgtttgtgtgcttattggatatttaaaatcaaaataaatttgatttaagttacttcactcatccacagaggttgcaatccagcctctgtttttcgataaaatagaggatgttgcgt Inter-species Hit: Count: 3 Min: 146 Max: 467 Len: 322 Subject: NC_008533_SPD_0460_SPD_0461|chaperone protein DnaK:chaperone protein DnaJ|POSITIVE:POSITIVE|[471412,472170]|759 HSP 1 e-value: 4.0E-22 bit: 107.0 Len: 93 Query Start:321 Query End:413 Subject Strand: POSITIVE Subject Start: 333 Subject End: 426 ................................................................................................................................................................................................................................................................................................................................attggaattttatagccaa-tactaagaagagacaaaaatatataaatgtttgtgtgcttattggatatttaaaatcaaaataaatttgattta..................................................................... ................................................................................................................................................................................................................................................................................................................................attggaattatataggcaaatactaagaagagacaaaaatatataaatatttctgtacttataggatatttaaaatcaaaataaagttaattta..................................................................... HSP 2 e-value: 5.0E-18 bit: 93.7 Len: 71 Query Start:146 Query End:216 Subject Strand: POSITIVE Subject Start: 146 Subject End: 216 .................................................................................................................................................ttagctgagcatgaaagttgtgttaaaaatgatgaggcggtaaggaattttgttacttcagtattgttgtc.......................................................................................................................................................................................................................................................................... .................................................................................................................................................ttagctgaacatgatagttgtatcaaaaatgatgaagcggtaaggaattttgttacctcagtattgttgtc.......................................................................................................................................................................................................................................................................... HSP 3 e-value: 5.0E-9 bit: 63.9 Len: 36 Query Start:432 Query End:467 Subject Strand: POSITIVE Subject Start: 435 Subject End: 470 ...............................................................................................................................................................................................................................................................................................................................................................................................................................................cagaggttgcaatccagcctctgtttttcgataaaa............... ...............................................................................................................................................................................................................................................................................................................................................................................................................................................cagaggttgcaacccagcctctgtttttcgataaaa............... Subject: NC_003098_spr0455_spr0456|molecular chaperone DnaK:Heat-shock protein (activation of DnaK)|POSITIVE:POSITIVE|[463882,464640]|759 HSP 1 e-value: 4.0E-22 bit: 107.0 Len: 93 Query Start:321 Query End:413 Subject Strand: POSITIVE Subject Start: 333 Subject End: 426 ................................................................................................................................................................................................................................................................................................................................attggaattttatagccaa-tactaagaagagacaaaaatatataaatgtttgtgtgcttattggatatttaaaatcaaaataaatttgattta..................................................................... ................................................................................................................................................................................................................................................................................................................................attggaattatataggcaaatactaagaagagacaaaaatatataaatatttctgtacttataggatatttaaaatcaaaataaagttaattta..................................................................... HSP 2 e-value: 5.0E-18 bit: 93.7 Len: 71 Query Start:146 Query End:216 Subject Strand: POSITIVE Subject Start: 146 Subject End: 216 .................................................................................................................................................ttagctgagcatgaaagttgtgttaaaaatgatgaggcggtaaggaattttgttacttcagtattgttgtc.......................................................................................................................................................................................................................................................................... .................................................................................................................................................ttagctgaacatgatagttgtatcaaaaatgatgaagcggtaaggaattttgttacctcagtattgttgtc.......................................................................................................................................................................................................................................................................... HSP 3 e-value: 5.0E-9 bit: 63.9 Len: 36 Query Start:432 Query End:467 Subject Strand: POSITIVE Subject Start: 435 Subject End: 470 ...............................................................................................................................................................................................................................................................................................................................................................................................................................................cagaggttgcaatccagcctctgtttttcgataaaa............... ...............................................................................................................................................................................................................................................................................................................................................................................................................................................cagaggttgcaacccagcctctgtttttcgataaaa............... Subject: NC_003028_SP_0518_SP_0519|hypothetical protein:dnaJ protein|POSITIVE:POSITIVE|[499457,500066]|610 HSP 1 e-value: 4.0E-22 bit: 107.0 Len: 93 Query Start:321 Query End:413 Subject Strand: POSITIVE Subject Start: 184 Subject End: 277 ................................................................................................................................................................................................................................................................................................................................attggaattttatagccaa-tactaagaagagacaaaaatatataaatgtttgtgtgcttattggatatttaaaatcaaaataaatttgattta..................................................................... ................................................................................................................................................................................................................................................................................................................................attggaattatataggcaaatactaagaagagacaaaaatatataaatatttctgtacttataggatatttaaaatcaaaataaagttaattta..................................................................... HSP 2 e-value: 1.0E-15 bit: 85.7 Len: 67 Query Start:150 Query End:216 Subject Strand: POSITIVE Subject Start: 1 Subject End: 67 .....................................................................................................................................................ctgagcatgaaagttgtgttaaaaatgatgaggcggtaaggaattttgttacttcagtattgttgtc.......................................................................................................................................................................................................................................................................... .....................................................................................................................................................ctgaacatgatagttgtatcaaaaatgatgaagcggtaaggaattttgttacctcagtattgttgtc.......................................................................................................................................................................................................................................................................... HSP 3 e-value: 5.0E-9 bit: 63.9 Len: 36 Query Start:432 Query End:467 Subject Strand: POSITIVE Subject Start: 286 Subject End: 321 ...............................................................................................................................................................................................................................................................................................................................................................................................................................................cagaggttgcaatccagcctctgtttttcgataaaa............... ...............................................................................................................................................................................................................................................................................................................................................................................................................................................cagaggttgcaacccagcctctgtttttcgataaaa............... Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUAGCUGAGCAUGAAAGUUGUGUUAAAAAUGAUGAGGCGGUAAGGAAUUUUGUUACUUCAGUAUUGUUGUCAGCGCUAGUAUCAGCACUAGUAUCAGCAACCAUACAAAAACCAGAAUUUAUAAUAUCGUUUAUAAUUGGCAUGGUUUGGGUUGUCUGUGUUUUCCUUCUAAUCUAUUGGAAUUUUAUAGCCAAUACUAAGAAGAGACAAAAAUAUAUAAAUGUUUGUGUGCUUAUUGGAUAUUUAAAAUCAAAAUAAAUUUGAUUUAAGUUACUUCACUCAUCCACAGAGGUUGCAAUCCAGCCUCUGUUUUUCGAUAAAA (((((..(((......)))..)))))....((((((.(((((((...(((((((.((((.((((.(((((..(..(((((......)))))..)..))))).))))..((((((....((((((......))))))......))))))..((((.(((((..((((............))))...))))).)))).....)))).))))))).(((((((....))))))))))))))........((((((((.....))))))))...........)))))).((((((((((.....))))))))))............ (-79.10) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUUUAUCGAAAAACAGAGGCUGGAUUGCAACCUCUGUGGAUGAGUGAAGUAACUUAAAUCAAAUUUAUUUUGAUUUUAAAUAUCCAAUAAGCACACAAACAUUUAUAUAUUUUUGUCUCUUCUUAGUAUUGGCUAUAAAAUUCCAAUAGAUUAGAAGGAAAACACAGACAACCCAAACCAUGCCAAUUAUAAACGAUAUUAUAAAUUCUGGUUUUUGUAUGGUUGCUGAUACUAGUGCUGAUACUAGCGCUGACAACAAUACUGAAGUAACAAAAUUCCUUACCGCCUCAUCAUUUUUAACACAACUUUCAUGCUCAGCUAA .......((((.(((((((.((.....)).))))))).((((((.(..((((...((((((((.....))))))))..................................((((((..((((..(((((((.........((((............))))....(((.(((((........((((.((((((......))))))....))))........))))))))....((((((((....))))))))....)))))))))))..))))))....))))..)))))))..............))))............ (-68.69) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 322 98 55 52 12 smit:SMT_550|5end_K02945_small_subunit_ribosomal_protein_S1_467660..467890_POSITIVE 316 103 67 59 12 smit:SMT_934|5end_pyridine_nucleotide-disulfide_oxidoreductase_885911..886141_POSITIVE 297 102 69 58 20 smit:SMT_452|5end_phosphomethylpyrimidine_kinase_390568..390798_NEGATIVE 296 315 274 132 92 smit:SMT_977|5end_conserved_hypothetical_protein,_Rrf2_family_protein_925689..925919_NEGATIVE 296 180 139 113 68 smit:SMT_904|5end_hypothetical_protein_852698..852928_NEGATIVE 281 120 71 143 89 smit:SMT_2074|5end_dTDP-glucose_4,6-dehydratase_1977021..1977251_POSITIVE 279 306 262 65 7 smit:SMT_459|5end_hypothetical_protein_397350..397580_POSITIVE 275 318 274 119 65 smit:SMT_771|5end_spermidine/putrescine_transport_system_permease_protein_PotB_682183..682413_NEGATIVE 271 302 269 181 141 smit:SMT_1414|5end_hypothetical_protein_1398446..1398676_NEGATIVE 271 114 71 101 50 smit:SMT_521|5end_K02005_HlyD_family_secretion_protein_445566..445796_POSITIVE 270 95 56 206 165 smit:SMT_794|5end_hypothetical_protein_703487..703717_NEGATIVE 270 176 139 180 142 smit:SMT_759|5end_K01992_ABC-2_type_transport_system_permease_protein_665712..665942_NEGATIVE 269 100 67 185 151 smit:SMT_1668|5end_K+_transport_system,_NAD-binding_component_1658288..1658518_NEGATIVE 268 76 33 185 144 smit:SMT_339|5end_K03687_molecular_chaperone_GrpE_277886..278116_POSITIVE 268 316 278 147 108 smit:SMT_236|5end_ribosomal_small_subunit_pseudouridine_synthase_A_174625..174855_POSITIVE 267 59 16 64 23 smit:SMT_1863|5end_glutamyl_aminopeptidase_1825628..1825858_POSITIVE 267 59 16 64 23 smit:SMT_1746|5end_glutamyl_aminopeptidase_1725785..1726015_POSITIVE 267 59 16 157 116 smit:SMT_379|5end_acetyltransferase,_GNAT_family_316095..316325_POSITIVE 266 97 55 119 72 smit:SMT_2045|5end_K+_transport_system,_NAD-binding_component_1956139..1956369_POSITIVE 266 316 274 223 177 smit:SMT_1493|5end_DNA-directed_RNA_polymerase,_beta'_subunit/160_kD_subunit_1475737..1475967_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 296 315 274 56 16 smit:SMT_978|3end_hypothetical_protein_925765..925915_NEGATIVE 281 120 71 116 62 smit:SMT_2073|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1977074..1977224_POSITIVE 267 59 16 65 24 smit:SMT_378|3end_hypothetical_protein_predicted_by_Glimmer/Critica_316083..316233_POSITIVE 265 177 137 65 21 smit:SMT_1402|3end_pyruvate_dehydrogenase_E2_component_(dihydrolipoamide_acetyltransferase)_1379238..1379388_POSITIVE 263 319 274 75 34 smit:SMT_1785|3end_phenylalanyl-tRNA_synthetase_beta_chain_1762327..1762477_NEGATIVE 263 180 140 45 1 smit:SMT_905|3end_hypothetical_protein_predicted_by_Glimmer/Critica_852766..852916_NEGATIVE 263 70 30 58 20 smit:SMT_640|3end_PTS_system,_lactose-specific_IIBC_component_545099..545249_NEGATIVE 257 168 139 141 113 smit:SMT_2042|3end_K04077_chaperonin_GroEL_(Hsp60)_1952960..1953110_NEGATIVE 257 170 138 90 51 smit:SMT_123|3end_hypothetical_protein_75623..75773_POSITIVE 254 186 142 88 42 smit:SMT_1214|3end_K03584_DNA_repair_protein_RecO_(recombination_protein_O)_1172652..1172802_NEGATIVE 253 180 137 102 55 smit:SMT_1702|3end_penicillin-binding_protein_2B_1686729..1686879_POSITIVE 253 98 57 135 94 smit:SMT_1404|3end_lipoate-protein_ligase_A_1382036..1382186_POSITIVE 253 103 63 78 39 smit:SMT_1210|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1170959..1171109_NEGATIVE 253 91 56 63 23 smit:SMT_280|3end_polysaccharide_biosynthesis_protein/putative_rhamnosyl_transferase_223628..223778_POSITIVE 251 100 51 80 19 smit:SMT_1480|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1462266..1462416_POSITIVE 248 313 271 92 43 smit:SMT_493|3end_K01996_branched-chain_amino_acid_transport_system_ATP-binding_protein_427927..428077_POSITIVE 246 315 288 82 49 smit:SMT_1805|3end_2,3,4,5-tetrahydropyridine-2-carboxylate_N-succinyltransferase,_putative_1781241..1781391_POSITIVE 246 315 288 82 49 smit:SMT_1634|3end_2,3,4,5-tetrahydropyridine-2-carboxylate_N-succinyltransferase,_putative_1621438..1621588_POSITIVE 246 314 281 87 53 smit:SMT_1620|3end_hypothetical_protein_1606695..1606845_NEGATIVE 245 318 274 97 41 smit:SMT_1780|3end_PTS_system,_glucose-specific_IIABC_component_1755960..1756110_NEGATIVE