Origin IGS: aaccatgcatgttttctaatttggaggtgcgatgatgaaaagcaaactacaatttcggatttttacgattcttgatgaggataagaaagaagaatatttacatgagatgcacttgaaaggttggaggtatagaacgagtcgttttggttttttctattttgaccaatgtcaaccagacgatgtcatctaccgtatctatgattctagatttcttaaaaaatataagcatgaactgcaagattttagaaatagaggttgggaattgatagaaagaggtttttattcaatttttcgtaaaccggcttctaatatacttccagaggatcaagtctatatgagtaaggggctcagatgggaagttatgcgatatagatttcgttcatgtgcagctactttcttaggtggtcttgttgtttgtatgagtttgtttcgagaagaactgtcgcaatctttctttgtcatttttgctctatatgcctttatgatttcttatttaattcatggctatttaaggcttaaaagggcataccgagtagataaacagtaaggttctaggtctttagactgatttttagcactcttggcaaaagagtgctaattttttgagtttttgtcttgacattctcttttaagggtgtataatagaatcataagttagcacttgagtgtatcgagtgctaatcgatcagacagagaggagtgatgag .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ctcatcactcctctctgtctgatcgattagcactcgatacactcaagtgctaacttatgattctattatacacccttaaaagagaatgtcaagacaaaaactcaaaaaattagcactcttttgccaagagtgctaaaaatcagtctaaagacctagaaccttactgtttatctactcggtatgcccttttaagccttaaatagccatgaattaaataagaaatcataaaggcatatagagcaaaaatgacaaagaaagattgcgacagttcttctcgaaacaaactcatacaaacaacaagaccacctaagaaagtagctgcacatgaacgaaatctatatcgcataacttcccatctgagccccttactcatatagacttgatcctctggaagtatattagaagccggtttacgaaaaattgaataaaaacctctttctatcaattcccaacctctatttctaaaatcttgcagttcatgcttatattttttaagaaatctagaatcatagatacggtagatgacatcgtctggttgacattggtcaaaatagaaaaaaccaaaacgactcgttctatacctccaacctttcaagtgcatctcatgtaaatattcttctttcttatcctcatcaagaatcgtaaaaatccgaaattgtagtttgcttttcatcatcgcacctccaaattagaaaacatgcatggtt Mask Tandem Repeat Region ================================================ aaccatgcatgttttctaatttggaggtgcgatgatgaaaagcaaactacaatttcggatttttacgattcttgatgaggataagaaagaagaatatttacatgagatgcacttgaaaggttggaggtatagaacgagtcgttttggttttttctattttgaccaatgtcaaccagacgatgtcatctaccgtatctatgattctagatttcttaaaaaatataagcatgaactgcaagattttagaaatagaggttgggaattgatagaaagaggtttttattcaatttttcgtaaaccggcttctaatatacttccagaggatcaagtctatatgagtaaggggctcagatgggaagttatgcgatatagatttcgttcatgtgcagctactttcttaggtggtcttgttgtttgtatgagtttgtttcgagaagaactgtcgcaatctttctttgtcatttttgctctatatgcctttatgatttcttatttaattcatggctatttaaggcttaaaagggcataccgagtagataaacagtaaggttctaggtctttagactgatttttagcactcttggcaaaagagtgctaattttttgagtttttgtcttgacattctcttttaagggtgtataatagaatcataagttagcacttgagtgtatcgagtgctaatcgatcagacagagaggagtgatgag Find is-nt database================================================ Query_seq: SMT_337:SMT_338|SMT_337:SMT_338:hypothetical protein:heat-inducible transcription repressor HrcA:->->:276258..276964 707 aaccatgcatgttttctaatttggaggtgcgatgatgaaaagcaaactacaatttcggatttttacgattcttgatgaggataagaaagaagaatatttacatgagatgcacttgaaaggttggaggtatagaacgagtcgttttggttttttctattttgaccaatgtcaaccagacgatgtcatctaccgtatctatgattctagatttcttaaaaaatataagcatgaactgcaagattttagaaatagaggttgggaattgatagaaagaggtttttattcaatttttcgtaaaccggcttctaatatacttccagaggatcaagtctatatgagtaaggggctcagatgggaagttatgcgatatagatttcgttcatgtgcagctactttcttaggtggtcttgttgtttgtatgagtttgtttcgagaagaactgtcgcaatctttctttgtcatttttgctctatatgcctttatgatttcttatttaattcatggctatttaaggcttaaaagggcataccgagtagataaacagtaaggttctaggtctttagactgatttttagcactcttggcaaaagagtgctaattttttgagtttttgtcttgacattctcttttaagggtgtataatagaatcataagttagcacttgagtgtatcgagtgctaatcgatcagacagagaggagtgatgag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_337:SMT_338|SMT_337:SMT_338:hypothetical protein:heat-inducible transcription repressor HrcA:->->:276258..276964 707 aaccatgcatgttttctaatttggaggtgcgatgatgaaaagcaaactacaatttcggatttttacgattcttgatgaggataagaaagaagaatatttacatgagatgcacttgaaaggttggaggtatagaacgagtcgttttggttttttctattttgaccaatgtcaaccagacgatgtcatctaccgtatctatgattctagatttcttaaaaaatataagcatgaactgcaagattttagaaatagaggttgggaattgatagaaagaggtttttattcaatttttcgtaaaccggcttctaatatacttccagaggatcaagtctatatgagtaaggggctcagatgggaagttatgcgatatagatttcgttcatgtgcagctactttcttaggtggtcttgttgtttgtatgagtttgtttcgagaagaactgtcgcaatctttctttgtcatttttgctctatatgcctttatgatttcttatttaattcatggctatttaaggcttaaaagggcataccgagtagataaacagtaaggttctaggtctttagactgatttttagcactcttggcaaaagagtgctaattttttgagtttttgtcttgacattctcttttaagggtgtataatagaatcataagttagcacttgagtgtatcgagtgctaatcgatcagacagagaggagtgatgag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_337:SMT_338|SMT_337:SMT_338:hypothetical protein:heat-inducible transcription repressor HrcA:->->:276258..276964 707 aaccatgcatgttttctaatttggaggtgcgatgatgaaaagcaaactacaatttcggatttttacgattcttgatgaggataagaaagaagaatatttacatgagatgcacttgaaaggttggaggtatagaacgagtcgttttggttttttctattttgaccaatgtcaaccagacgatgtcatctaccgtatctatgattctagatttcttaaaaaatataagcatgaactgcaagattttagaaatagaggttgggaattgatagaaagaggtttttattcaatttttcgtaaaccggcttctaatatacttccagaggatcaagtctatatgagtaaggggctcagatgggaagttatgcgatatagatttcgttcatgtgcagctactttcttaggtggtcttgttgtttgtatgagtttgtttcgagaagaactgtcgcaatctttctttgtcatttttgctctatatgcctttatgatttcttatttaattcatggctatttaaggcttaaaagggcataccgagtagataaacagtaaggttctaggtctttagactgatttttagcactcttggcaaaagagtgctaattttttgagtttttgtcttgacattctcttttaagggtgtataatagaatcataagttagcacttgagtgtatcgagtgctaatcgatcagacagagaggagtgatgag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 3 Min: 35 Max: 541 Len: 507 Subject: UniRef90_Q97S74 Cluster: Hypothetical protein; n=4; Streptococcus pneumoniae|Rep: Hypothetical protein - Streptococcus pneumoniae HSP 1 e-value: 2.0E-44 bit: 181.0 Len: 333 Query Start:209 Query End:541 Subject Strand: null Subject Start: 5 Subject End: 115 ................................................................................................................................................................................................................ F L K K Y K H E L Q D F R N R G W E L I E R G F Y S I F R K P A S N I L P E D Q V Y M S K G L R W E V M R Y R F R S C A A T F L G G L V V C M S L F R E E L S Q S F F V I F A L Y A F M I S Y L I H G Y L R L K R A Y R V D K ...................................................................................................................................................................... ................................................................................................................................................................................................................ F L K K Y K H E L Q D F R D R G W E L I G A G S C S I L R K S S S D L L P E D Q V Y M S K G L K W E V M R S R L R S C T A T F S G G L V V C M S L F R E D L S M S F F L I F V L Y A F L I S Y L I Y G Y F R L K R K Y R V D E ...................................................................................................................................................................... Subject: UniRef90_A3CQC7 Cluster: Hypothetical protein; n=1; Streptococcus sanguinis SK36|Rep: Hypothetical protein - Streptococcus sanguinis (strain SK36) HSP 1 e-value: 2.0E-8 bit: 61.6 Len: 396 Query Start:35 Query End:430 Subject Strand: null Subject Start: 1 Subject End: 140 .................................. M K S K L Q F R I F T I L D E D K K E E Y L H E M H L K G W R Y R T S R F G F F Y - - - - - - F D Q C Q P D D V I Y R I Y D S R F L K K Y K H E - - L Q D F R N R G W E L I E R - G F Y S I F R K P A S N I L P E D Q V Y M S K G L R W E V M R Y R F R S C A A T F L G G - L V V C M S L F ..................................................................................................................................................................................................................................................................................... .................................. M E K K I V Y K I F T I A D Y D R E A A Y F K K M H S Q G W R L K K V S Y S P F L V A V R Y T F E A C P P Q E M A Y Q L - D F R P M K K A E Q E S Y Y Q L F E D C G W E H I T N F N Q F S Y F R K P V S Q L D A E S D L E I Y N D - A W S K L E M V K R L L V W R F L P A I M V L C V T T Y ..................................................................................................................................................................................................................................................................................... Subject: UniRef90_Q2E6B0 Cluster: Hypothetical protein; n=1; Bacillus cereus subsp. cytotoxis NVH 391-98|Rep: Hypothetical protein - Bacillus cereus subsp. cytotoxis NVH 391-98 HSP 1 e-value: 1.0E-6 bit: 55.8 Len: 483 Query Start:35 Query End:517 Subject Strand: null Subject Start: 1 Subject End: 166 .................................. M K S K L Q F R I F T I L D E D K K E E Y L H E M H L K G W R Y R T S R F G F F Y F D Q C Q P D D V I Y R I - - - Y D - S R F L K K Y K H E L Q D F R N R G W E L I E - - - R G F Y S I F R K P A S N I L P E D Q V Y M S K G L R W E V M R Y R F R S C A A T F L G G L - V V C M S L F R E E L S Q S F F V I F A L Y A F M I S Y L I H G Y L R L .............................................................................................................................................................................................. .................................. M E I K K T F K F F T A W N L E K E E A Y L R E M H Q K G W A F Q K Y H F - M Y T F K K T E P K D V V Y K A D F N F D F Q K S L K N Q R E Y L E I Y E M S G W K Y V S S F T K W H Y F C K E V V D K N E L P D - - I Y S E T E T R I E K L K D L L N H L V I I F L S I L P A I S I S C L E S P A N K L S I I I K G F I G F V V C I H M Y M F V R L .............................................................................................................................................................................................. aaccatgcatgttttctaatttggaggtgcgatgnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnncagtaaggttctaggtctttagactgatttttagcactcttggcaaaagagtgctaattttttgagtttttgtcttgacattctcttttaagggtgtataatagaatcataagttagcacttgagtgtatcgagtgctaatcgatcagacagagaggagtgatgag Predict ORF larger than 30AA ================================================ Protein_Len: 45 Strand: + Start: 129 End: 263 ................................................................................................................................ M E R V V L V F S I L T N V N Q T M S S T V S M I L D F L K N I S M N C K I L E I E V G N ............................................................................................................................................................................................................................................................................................................................................................................................................................................................ Protein_Len: 48 Strand: + Start: 373 End: 516 .................................................................................................................................................................................................................................................................................................................................................................................... M S F M C S Y F L R W S C C L Y E F V S R R T V A I F L C H F C S I C L Y D F L F N S W L F K A ............................................................................................................................................................................................... Protein_Len: 60 Strand: + Start: 32 End: 544 ............................... M M K S K L Q F R I F T I L D E D K K E E Y L H E M H L K G W R Y R T S R F G F F Y F D Q C Q P D D V I Y R I Y D S R F ................................................................................................................................................................... Protein_Len: 51 Strand: - Start: 334 End: 486 ............................................................................................................................................................................................................................................................................................................................................. I L L P S L H S T I R Y L N R E H A A V K K P P R T T Q I L K N R S S S D C D K K T M K A R Y A K I M ............................................................................................................................................................................................................................. Protein_Len: 60 Strand: - Start: 101 End: 283 .................................................................................................... L H V Q F T P P I S R T T K T K E I K V L T L W V I D D V T D I I R S K K F F I L M F Q L I K S I S T P F Q Y F S T K M ........................................................................................................................................................................................................................................................................................................................................................................................................................................ Protein_Len: 37 Strand: - Start: 66 End: 176 ................................................................. S E Q H P Y S L L I N V H S A S S L N S T Y F S D N Q N K R N Q G I D V M ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 30 Strand: - Start: 19 End: 108 .................. N P P A I I F L L S C N R I K V I R S S S L F S S Y K C S M ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... aaccatgcatgttttctaatttggaggtgcgatgnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnncagtaaggttctaggtctttagactgatttttagcactcttggcaaaagagtgctaattttttgagtttttgtcttgacattctcttttaagggtgtataatagaatcataagttagcacttgagtgtatcgagtgctaatcgatcagacagagaggagtgatgag Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aaccatgcatgttttctaatttggaggtgcgatgnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnncagtaaggttctaggtctttagactgatttttagcactcttggcaaaagagtgctaattttttgagtttttgtcttgacattctcttttaagggtgtataatagaatcataagttagcacttgagtgtatcgagtgctaatcgatcagacagagaggagtgatgag Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 100 HP_score: -12.5 Tail_Score: -5.30575 Start: 572 End: 598 Full_Region: TCTTTAGACTGATTT TTAGCACTCTT GGCAA AAGAGTGCTAA TTTTTTGAGTTTTTG ...........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TTAGCACTCTTGGCAAAAGAGTGCTAA............................................................................................................. Find igs database================================================ Query_seq: SMT_337:SMT_338|SMT_337:SMT_338:hypothetical protein:heat-inducible transcription repressor HrcA:->->:276258..276964 707 aaccatgcatgttttctaatttggaggtgcgatgnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnncagtaaggttctaggtctttagactgatttttagcactcttggcaaaagagtgctaattttttgagtttttgtcttgacattctcttttaagggtgtataatagaatcataagttagcacttgagtgtatcgagtgctaatcgatcagacagagaggagtgatgag Intra-Species Hit: Count: 1 Min: 1 Max: 707 Len: 707 Subject: smit_SMT_337_SMT_338|hypothetical protein:heat-inducible transcription repressor HrcA|POSITIVE:POSITIVE|[276258,276964]|707 HSP 1 e-value: 8.0E-89 bit: 329.0 Len: 166 Query Start:542 Query End:707 Subject Strand: POSITIVE Subject Start: 542 Subject End: 707 .............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................cagtaaggttctaggtctttagactgatttttagcactcttggcaaaagagtgctaattttttgagtttttgtcttgacattctcttttaagggtgtataatagaatcataagttagcacttgagtgtatcgagtgctaatcgatcagacagagaggagtgatgag .............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................cagtaaggttctaggtctttagactgatttttagcactcttggcaaaagagtgctaattttttgagtttttgtcttgacattctcttttaagggtgtataatagaatcataagttagcacttgagtgtatcgagtgctaatcgatcagacagagaggagtgatgag HSP 2 e-value: 2.0E-9 bit: 65.9 Len: 33 Query Start:1 Query End:33 Subject Strand: POSITIVE Subject Start: 1 Subject End: 33 aaccatgcatgttttctaatttggaggtgcgat.................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. aaccatgcatgttttctaatttggaggtgcgat.................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Inter-species Hit: Count: 7 Min: 546 Max: 707 Len: 162 Subject: NC_008533_SPD_0457_SPD_0458|hypothetical protein:heat-inducible transcription repressor HrcA|POSITIVE:POSITIVE|[467360,467522]|163 HSP 1 e-value: 3.0E-60 bit: 234.0 Len: 162 Query Start:546 Query End:707 Subject Strand: POSITIVE Subject Start: 2 Subject End: 163 .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................aaggttctaggtctttagactgatttttagcactcttggcaaaagagtgctaattttttgagtttttgtcttgacattctcttttaagggtgtataatagaatcataagttagcacttgagtgtatcgagtgctaatcgatcagacagagaggagtgatgag .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................aaggttctaggtcttcagattgatttttagcactctttataaaagagtgctaattttttgagtttttgtcttgacattctcttctaagggtgtataatagaatcataagttagcacttggatgcattgagtgctaattgatcagacagagaggagtgatgag Subject: NC_003028_SP_0514_SP_0515|hypothetical protein:heat-inducible transcription repressor|POSITIVE:POSITIVE|[495248,495410]|163 HSP 1 e-value: 3.0E-60 bit: 234.0 Len: 162 Query Start:546 Query End:707 Subject Strand: POSITIVE Subject Start: 2 Subject End: 163 .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................aaggttctaggtctttagactgatttttagcactcttggcaaaagagtgctaattttttgagtttttgtcttgacattctcttttaagggtgtataatagaatcataagttagcacttgagtgtatcgagtgctaatcgatcagacagagaggagtgatgag .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................aaggttctaggtcttcagattgatttttagcactcttgataaaagagtgctaattttttgagtttttgtcttgacattctcttctaagggtgtataatagaatcatgagttagcacttggatgcattgagtgctaattgatcagacagagaggagtgatgag Subject: NC_003098_spr0452_spr0453|hypothetical protein:heat-inducible transcription repressor|POSITIVE:POSITIVE|[459830,459959]|130 HSP 1 e-value: 2.0E-46 bit: 188.0 Len: 119 Query Start:546 Query End:664 Subject Strand: POSITIVE Subject Start: 2 Subject End: 120 .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................aaggttctaggtctttagactgatttttagcactcttggcaaaagagtgctaattttttgagtttttgtcttgacattctcttttaagggtgtataatagaatcataagttagcacttg........................................... .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................aaggttctaggtcttcagattgatttttagcactctttataaaagagtgctaattttttgagtttttgtcttgacattctcttctaagggtgtataatagaatcataagttagcacttg........................................... Subject: NC_009009_SSA_2009_SSA_2010|Heat shock transcription repressor HrcA, putative:ABC-type multidrug transport system, permease component, putative|NEGATIVE:NEGATIVE|[2011223,2011385]|163 HSP 1 e-value: 2.0E-27 bit: 125.0 Len: 135 Query Start:551 Query End:685 Subject Strand: NEGATIVE Subject Start: 29 Subject End: 163 ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tctaggtctttagactgatttttagcactcttggcaaaagagtgctaattttttgagtttttgtcttgacattctcttttaagggtgtataatagaatcataagttagcacttgagtgtatcgagtgctaatcga...................... ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tctaggtcgctagactgatttttagcactcttgtaaaaagagtgctaattttttgagtttttgtcttgactttggagtagaagagtgtataatagaatcatgagttagcactcgaggtgattgagtgctaatcga...................... Subject: NC_008532_STER_0160_STER_0161|N-acetylmuramidase:Transcriptional regulator of heat shock gene|POSITIVE:POSITIVE|[133174,133363]|190 HSP 1 e-value: 7.0E-6 bit: 54.0 Len: 55 Query Start:550 Query End:604 Subject Strand: POSITIVE Subject Start: 34 Subject End: 88 .....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ttctaggtctttagactgatttttagcactcttggcaaaagagtgctaatttttt....................................................................................................... .....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ttctaggtctctagatgtatttttagcactctttcaaaaagagtgctaatttttt....................................................................................................... HSP 2 e-value: 7.0E-6 bit: 54.0 Len: 27 Query Start:634 Query End:660 Subject Strand: POSITIVE Subject Start: 121 Subject End: 147 .........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ggtgtataatagaatcataagttagca............................................... .........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ggtgtataatagaatcataagttagca............................................... Subject: NC_006449_str0117_str0118|peptidoglycan hydrolase:heat-inducible transcription repressor|POSITIVE:POSITIVE|[121270,121461]|192 HSP 1 e-value: 7.0E-6 bit: 54.0 Len: 55 Query Start:550 Query End:604 Subject Strand: POSITIVE Subject Start: 34 Subject End: 88 .....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ttctaggtctttagactgatttttagcactcttggcaaaagagtgctaatttttt....................................................................................................... .....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ttctaggtctctagatgtatttttagcactctttcaaaaagagtgctaatttttt....................................................................................................... HSP 2 e-value: 7.0E-6 bit: 54.0 Len: 27 Query Start:634 Query End:660 Subject Strand: POSITIVE Subject Start: 123 Subject End: 149 .........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ggtgtataatagaatcataagttagca............................................... .........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ggtgtataatagaatcataagttagca............................................... Subject: NC_006448_stu0117_stu0118|peptidoglycan hydrolase:heat-inducible transcription repressor|POSITIVE:POSITIVE|[121246,121434]|189 HSP 1 e-value: 7.0E-6 bit: 54.0 Len: 55 Query Start:550 Query End:604 Subject Strand: POSITIVE Subject Start: 34 Subject End: 88 .....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ttctaggtctttagactgatttttagcactcttggcaaaagagtgctaatttttt....................................................................................................... .....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ttctaggtctctagatgtatttttagcactctttcaaaaagagtgctaatttttt....................................................................................................... HSP 2 e-value: 7.0E-6 bit: 54.0 Len: 27 Query Start:634 Query End:660 Subject Strand: POSITIVE Subject Start: 120 Subject End: 146 .........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ggtgtataatagaatcataagttagca............................................... .........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ggtgtataatagaatcataagttagca............................................... Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAGGUUCUAGGUCUUUAGACUGAUUUUUAGCACUCUUGGCAAAAGAGUGCUAAUUUUUUGAGUUUUUGUCUUGACAUUCUCUUUUAAGGGUGUAUAAUAGAAUCAUAAGUUAGCACUUGAGUGUAUCGAGUGCUAAUCGAUCAGACAGAGAGGAGUGAUGAG ....((((...(((((.(.((((((.(((((((((...(((...((((((((((((..(((..((((((....(((((((......)))))))...))))))))).))))))))))))...)))...)))))))))..)))))).))))))))))....... (-52.30) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CUCAUCACUCCUCUCUGUCUGAUCGAUUAGCACUCGAUACACUCAAGUGCUAACUUAUGAUUCUAUUAUACACCCUUAAAAGAGAAUGUCAAGACAAAAACUCAAAAAAUUAGCACUCUUUUGCCAAGAGUGCUAAAAAUCAGUCUAAAGACCUAGAACCUU ...........(((..((((......((((((((.((.....)).))))))))....(((((...................(((..(((....)))....)))......(((((((((((.....))))))))))).)))))......))))..)))..... (-35.40) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 340 50 5 158 102 smit:SMT_845|5end_hypothetical_protein_791868..792098_NEGATIVE 321 157 113 219 176 smit:SMT_1982|5end_conserved_hypothetical_protein_1913036..1913266_POSITIVE 321 157 113 192 149 smit:SMT_1972|5end_uncharacterized_conserved_phage_related_protein_1903662..1903892_POSITIVE 321 157 113 207 164 smit:SMT_1691|5end_putative_deoxyribonuclease_1674978..1675208_POSITIVE 321 157 113 170 127 smit:SMT_325|5end_K02029_polar_amino_acid_transport_system_permease_protein_263023..263253_NEGATIVE 295 154 113 228 189 smit:SMT_1156|5end_K07485_transposase_1099938..1100168_POSITIVE 293 159 116 121 76 smit:SMT_2071|5end_phosphoenolpyruvate_carboxylase_1975887..1976117_NEGATIVE 289 160 122 191 152 smit:SMT_484|5end_hypothetical_protein_420180..420410_NEGATIVE 283 159 116 73 32 smit:SMT_761|5end_prephenate_dehydrogenase_667150..667380_NEGATIVE 283 159 116 73 32 smit:SMT_752|5end_prephenate_dehydrogenase_656791..657021_NEGATIVE 275 39 1 161 120 smit:SMT_621|5end_K03346_replication_initiation_and_membrane_attachment_protein_528794..529024_POSITIVE 275 39 1 85 44 smit:SMT_620|5end_hypothetical_protein_predicted_by_Glimmer/Critica_528718..528948_POSITIVE 274 157 111 204 156 smit:SMT_1558|5end_hypothetical_protein_1544058..1544288_NEGATIVE 272 70 24 193 135 smit:SMT_821|5end_UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-_diaminopimelate_ligase_732775..733005_POSITIVE 262 95 58 150 114 smit:SMT_1397|5end_mutator_MutT_protein_1372727..1372957_POSITIVE 259 160 123 48 15 smit:SMT_1138|5end_hypothetical_protein_1079547..1079777_NEGATIVE 258 76 32 57 4 smit:SMT_675|5end_DNA_repair_protein_582977..583207_NEGATIVE 256 160 111 174 136 smit:SMT_112|5end_hypothetical_protein_predicted_by_Glimmer/Critica_61473..61703_NEGATIVE 248 148 103 154 105 smit:SMT_943|5end_hypothetical_protein_894765..894995_NEGATIVE 239 94 54 113 66 smit:SMT_1696|5end_hypothetical_protein_1678182..1678412_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 289 160 122 142 103 smit:SMT_485|3end_hypothetical_protein_420229..420379_NEGATIVE 283 159 116 63 22 smit:SMT_753|3end_chorismate_synthase_656801..656951_NEGATIVE 262 95 58 140 104 smit:SMT_1396|3end_uracil-DNA_glycosylase_1372797..1372947_POSITIVE 248 148 103 151 102 smit:SMT_944|3end_PhnA_protein_894768..894918_NEGATIVE 239 94 54 150 103 smit:SMT_1695|3end_ribulose-phosphate_3-epimerase_1678299..1678449_POSITIVE 231 49 1 136 74 smit:SMT_1487|3end_hypothetical_protein_1467076..1467226_POSITIVE 231 85 43 114 70 smit:SMT_360|3end_K07742_hypothetical_protein_296001..296151_POSITIVE 228 157 111 148 92 smit:SMT_807|3end_hypothetical_protein_predicted_by_Glimmer/Critica_716708..716858_NEGATIVE 225 52 18 148 106 smit:SMT_1898|3end_aminotransferase_(class_V),_putative_1848877..1849027_NEGATIVE 224 85 48 146 106 smit:SMT_1593|3end_ABC-type_oligopeptide_transport_system,_periplasmic_component_1583485..1583635_POSITIVE 224 149 109 127 90 smit:SMT_244|3end_PTS_system,_mannose-specific_IIAB_component_180976..181126_NEGATIVE 223 148 101 126 89 smit:SMT_1786|3end_phenylalanyl-tRNA_synthetase_beta_chain_1763235..1763385_NEGATIVE 221 49 3 144 83 smit:SMT_1426|3end_K03095_SprT-like_protein_1410864..1411014_NEGATIVE 221 157 114 66 12 smit:SMT_812|3end_acetyltransferase,_GNAT_family_721664..721814_NEGATIVE 220 41 20 22 2 smit:SMT_1915|3end_pyridoxine_biosynthesis_protein_1860618..1860768_POSITIVE 219 56 20 150 121 smit:SMT_1588|3end_UDP-N-acetylglucosamine_1-carboxyvinyltransferase_1578425..1578575_POSITIVE 219 88 48 61 24 smit:SMT_1109|3end_hypothetical_protein_1049362..1049512_NEGATIVE 217 155 113 51 3 smit:SMT_822|3end_putative_mucus_binding_protein_734047..734197_NEGATIVE 216 40 3 99 63 smit:SMT_1379|3end_thymidylate_kinase_1356800..1356950_NEGATIVE 214 67 21 69 22 smit:SMT_277|3end_capsular_polysaccharide_biosynthesis_homolog_YwqC_220333..220483_POSITIVE