Origin IGS: aatatctaccagcgcccacgaccagctattttacacttggttgaggagaaggattttccttcccaagcggccattctctgnnnnnttaattaattaannnnncagagcttgtttcaggagagagtgagcatccaactaatgaagctgacaagcagcatgaaggagaacatgctagagaaaataagccagaaaaggcagaaggagtagcgacagcatctgaaactgcttcgccagcaagcaatgaagctgcaactactgaaactgcagaggcagtaagcgtagctaaaccagaggaaaaaccaagtgag .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ctcacttggtttttcctctggtttagctacgcttactgcctctgcagtttcagtagttgcagcttcattgcttgctggcgaagcagtttcagatgctgtcgctactccttctgccttttctggcttattttctctagcatgttctccttcatgctgcttgtcagcttcattagttggatgctcactctctcctgaaacaagctctgnnnnnttaattaattaannnnncagagaatggccgcttgggaaggaaaatccttctcctcaaccaagtgtaaaatagctggtcgtgggcgctggtagatatt Mask Tandem Repeat Region ================================================ aatatctaccagcgcccacgaccagctattttacacttggttgaggagaaggattttccttcccaagcggccattctctgnnnnnttaattaattaannnnncagagcttgtttcaggagagagtgagcatccaactaatgaagctgacaagcagcatgaaggagaacatgctagagaaaataagccagaaaaggcagaaggagtagcgacagcatctgaaactgcttcgccagcaagcaatgaagctgcaactactgaaactgcagaggcagtaagcgtagctaaaccagaggaaaaaccaagtgag Find is-nt database================================================ Query_seq: SMT_330:SMT_331|SMT_330:SMT_331:membrane-associated phospholipid phosphatase:endo-beta-N-acetylglucosaminidase:->->:266248..266555 308 aatatctaccagcgcccacgaccagctattttacacttggttgaggagaaggattttccttcccaagcggccattctctgnnnnnttaattaattaannnnncagagcttgtttcaggagagagtgagcatccaactaatgaagctgacaagcagcatgaaggagaacatgctagagaaaataagccagaaaaggcagaaggagtagcgacagcatctgaaactgcttcgccagcaagcaatgaagctgcaactactgaaactgcagaggcagtaagcgtagctaaaccagaggaaaaaccaagtgag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_330:SMT_331|SMT_330:SMT_331:membrane-associated phospholipid phosphatase:endo-beta-N-acetylglucosaminidase:->->:266248..266555 308 aatatctaccagcgcccacgaccagctattttacacttggttgaggagaaggattttccttcccaagcggccattctctgnnnnnttaattaattaannnnncagagcttgtttcaggagagagtgagcatccaactaatgaagctgacaagcagcatgaaggagaacatgctagagaaaataagccagaaaaggcagaaggagtagcgacagcatctgaaactgcttcgccagcaagcaatgaagctgcaactactgaaactgcagaggcagtaagcgtagctaaaccagaggaaaaaccaagtgag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_330:SMT_331|SMT_330:SMT_331:membrane-associated phospholipid phosphatase:endo-beta-N-acetylglucosaminidase:->->:266248..266555 308 aatatctaccagcgcccacgaccagctattttacacttggttgaggagaaggattttccttcccaagcggccattctctgnnnnnttaattaattaannnnncagagcttgtttcaggagagagtgagcatccaactaatgaagctgacaagcagcatgaaggagaacatgctagagaaaataagccagaaaaggcagaaggagtagcgacagcatctgaaactgcttcgccagcaagcaatgaagctgcaactactgaaactgcagaggcagtaagcgtagctaaaccagaggaaaaaccaagtgag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aatatctaccagcgcccacgaccagctattttacacttggttgaggagaaggattttccttcccaagcggccattctctgnnnnnttaattaattaannnnncagagcttgtttcaggagagagtgagcatccaactaatgaagctgacaagcagcatgaaggagaacatgctagagaaaataagccagaaaaggcagaaggagtagcgacagcatctgaaactgcttcgccagcaagcaatgaagctgcaactactgaaactgcagaggcagtaagcgtagctaaaccagaggaaaaaccaagtgag Predict ORF larger than 30AA ================================================ Protein_Len: 31 Strand: + Start: 2 End: 94 . M S T S A H D Q L F Y T W L R R R I F L P K R P F S X X L I N ...................................................................................................................................................................................................................... Protein_Len: 32 Strand: + Start: 109 End: 204 ............................................................................................................ M F Q E R V S I Q L M K L T S S M K E N M L E K I S Q K R Q K E ........................................................................................................ Protein_Len: 60 Strand: + Start: 93 End: 308 ............................................................................................ M X X X E L V S G E S E H P T N E A D K Q H E G E H A R E N K P E K A E G V A T A S E T A S P A S N E A A T T E T A E A Protein_Len: 42 Strand: - Start: 117 End: 242 .................................................................................................................... S L T L M W S I F S V L L M F S F M S S F I L W F L C F S Y R C C R F S S R W C A M .................................................................. Protein_Len: 51 Strand: - Start: 89 End: 241 ........................................................................................ N I L X X S S T E P S L S C G V L S A S L C C S P S C A L S F L G S F A S P T A V A D S V A E G A L M ................................................................... aatatctaccagcgcccacgaccagctattttacacttggttgaggagaaggattttccttcccaagcggccattctctgnnnnnttaattaattaannnnncagagcttgtttcaggagagagtgagcatccaactaatgaagctgacaagcagcatgaaggagaacatgctagagaaaataagccagaaaaggcagaaggagtagcgacagcatctgaaactgcttcgccagcaagcaatgaagctgcaactactgaaactgcagaggcagtaagcgtagctaaaccagaggaaaaaccaagtgag Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aatatctaccagcgcccacgaccagctattttacacttggttgaggagaaggattttccttcccaagcggccattctctgnnnnnttaattaattaannnnncagagcttgtttcaggagagagtgagcatccaactaatgaagctgacaagcagcatgaaggagaacatgctagagaaaataagccagaaaaggcagaaggagtagcgacagcatctgaaactgcttcgccagcaagcaatgaagctgcaactactgaaactgcagaggcagtaagcgtagctaaaccagaggaaaaaccaagtgag Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: SMT_330:SMT_331|SMT_330:SMT_331:membrane-associated phospholipid phosphatase:endo-beta-N-acetylglucosaminidase:->->:266248..266555 308 aatatctaccagcgcccacgaccagctattttacacttggttgaggagaaggattttccttcccaagcggccattctctgnnnnnttaattaattaannnnncagagcttgtttcaggagagagtgagcatccaactaatgaagctgacaagcagcatgaaggagaacatgctagagaaaataagccagaaaaggcagaaggagtagcgacagcatctgaaactgcttcgccagcaagcaatgaagctgcaactactgaaactgcagaggcagtaagcgtagctaaaccagaggaaaaaccaagtgag Intra-Species Hit: Count: 1 Min: 1 Max: 308 Len: 308 Subject: smit_SMT_330_SMT_331|membrane-associated phospholipid phosphatase:endo-beta-N- acetylglucosaminidase|POSITIVE:POSITIVE|[266248, 266555]|308 HSP 1 e-value: 1.0E-156 bit: 551.0 Len: 308 Query Start:1 Query End:308 Subject Strand: POSITIVE Subject Start: 1 Subject End: 308 aatatctaccagcgcccacgaccagctattttacacttggttgaggagaaggattttccttcccaagcggccattctctgnnnnnttaattaattaannnnncagagcttgtttcaggagagagtgagcatccaactaatgaagctgacaagcagcatgaaggagaacatgctagagaaaataagccagaaaaggcagaaggagtagcgacagcatctgaaactgcttcgccagcaagcaatgaagctgcaactactgaaactgcagaggcagtaagcgtagctaaaccagaggaaaaaccaagtgag aatatctaccagcgcccacgaccagctattttacacttggttgaggagaaggattttccttcccaagcggccattctctgnnnnnttaattaattaannnnncagagcttgtttcaggagagagtgagcatccaactaatgaagctgacaagcagcatgaaggagaacatgctagagaaaataagccagaaaaggcagaaggagtagcgacagcatctgaaactgcttcgccagcaagcaatgaagctgcaactactgaaactgcagaggcagtaagcgtagctaaaccagaggaaaaaccaagtgag Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAUAUCUACCAGCGCCCACGACCAGCUAUUUUACACUUGGUUGAGGAGAAGGAUUUUCCUUCCCAAGCGGCCAUUCUCUGNNNNNUUAAUUAAUUAANNNNNCAGAGCUUGUUUCAGGAGAGAGUGAGCAUCCAACUAAUGAAGCUGACAAGCAGCAUGAAGGAGAACAUGCUAGAGAAAAUAAGCCAGAAAAGGCAGAAGGAGUAGCGACAGCAUCUGAAACUGCUUCGCCAGCAAGCAAUGAAGCUGCAACUACUGAAACUGCAGAGGCAGUAAGCGUAGCUAAACCAGAGGAAAAACCAAGUGAG ..........(((...........)))......(((((((((..((.(((((.....))))))).........(((((((......................((..(((((((((.((.....((.(((.((........)).))).))....((((((........))))))............)).)))..(((.((((.(((..((........))..))).))))))).))))))..)).((((((...(((((...((....)).)))))...))))))....)))))))..))))))))).. (-83.20) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CUCACUUGGUUUUUCCUCUGGUUUAGCUACGCUUACUGCCUCUGCAGUUUCAGUAGUUGCAGCUUCAUUGCUUGCUGGCGAAGCAGUUUCAGAUGCUGUCGCUACUCCUUCUGCCUUUUCUGGCUUAUUUUCUCUAGCAUGUUCUCCUUCAUGCUGCUUGUCAGCUUCAUUAGUUGGAUGCUCACUCUCUCCUGAAACAAGCUCUGNNNNNUUAAUUAAUUAANNNNNCAGAGAAUGGCCGCUUGGGAAGGAAAAUCCUUCUCCUCAACCAAGUGUAAAAUAGCUGGUCGUGGGCGCUGGUAGAUAUU ..(((((((((........(((..(((...)))....)))...((((....((((((.(((((.((((((((((....).)))))))....)).))))).))))))....)))).......((((....((((((.(((((........))))).((((((.(((((((.....)))).)))...((......)).))))))...........................)))))).))))....(((((((.....)))))))...))))))))).......((..(.((....)))..))....... (-86.80) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 383 68 22 150 99 smit:SMT_1260|5end_hypothetical_protein_1225517..1225747_POSITIVE 364 247 204 180 126 smit:SMT_1201|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1163920..1164150_NEGATIVE 335 294 261 192 160 smit:SMT_2023|5end_tryptophan_synthase,_alpha_subunit_1939315..1939545_POSITIVE 335 252 215 51 5 smit:SMT_274|5end_ABC_transporter_substrate-binding_protein_-_oligopeptide_transport_215221..215451_POSITIVE 325 294 261 211 178 smit:SMT_2015|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1932655..1932885_POSITIVE 325 294 261 193 160 smit:SMT_1818|5end_tryptophan_synthase,_alpha_subunit_1792190..1792420_POSITIVE 323 269 223 181 130 smit:SMT_1647|5end_3-isopropylmalate_dehydratase_small_subunit_1633403..1633633_POSITIVE 322 258 217 42 1 smit:SMT_1224|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1188079..1188309_NEGATIVE 318 250 203 64 5 smit:SMT_297|5end_ABC_transporter_substrate-binding_protein_-_oligopeptide_transport_238547..238777_POSITIVE 316 76 35 196 157 smit:SMT_1398|5end_dihydroorotase_1373204..1373434_POSITIVE 314 208 161 55 10 smit:SMT_997|5end_integral_membrane_protein_938243..938473_POSITIVE 312 258 211 149 97 smit:SMT_1915|5end_pyridoxine_biosynthesis_protein_1860329..1860559_POSITIVE 311 267 224 60 22 smit:SMT_563|5end_thioredoxin_reductase_480988..481218_POSITIVE 310 69 21 210 162 smit:SMT_922|5end_RNA-binding_protein_876566..876796_NEGATIVE 309 251 217 196 161 smit:SMT_1982|5end_conserved_hypothetical_protein_1913036..1913266_POSITIVE 309 251 217 184 149 smit:SMT_1691|5end_putative_deoxyribonuclease_1674978..1675208_POSITIVE 309 251 217 209 174 smit:SMT_1156|5end_K07485_transposase_1099938..1100168_POSITIVE 309 235 194 55 14 smit:SMT_181|5end_50S_ribosomal_protein_L24_120125..120355_POSITIVE 305 250 201 211 149 smit:SMT_2051|5end_enoyl-CoA_hydratase_1960639..1960869_NEGATIVE 304 239 196 65 4 smit:SMT_2089|5end_dTDP-4-dehydrorhamnose_3,5-epimerase_related_enzyme_1984987..1985217_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 383 68 22 131 80 smit:SMT_1259|3end_hypothetical_protein_1225578..1225728_POSITIVE 314 205 167 137 93 smit:SMT_1070|3end_hypothetical_protein_1009644..1009794_NEGATIVE 312 258 211 64 12 smit:SMT_1914|3end_K03696_ATP-dependent_Clp_protease_ATP-binding_subunit_ClpC_1860324..1860474_POSITIVE 306 241 204 146 101 smit:SMT_1202|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1163945..1164095_NEGATIVE 304 239 196 64 3 smit:SMT_2088|3end_glucose-1-phosphate_thymidylyltransferase_1985066..1985216_POSITIVE 304 239 196 64 3 smit:SMT_290|3end_glucose-1-phosphate_thymidylyltransferase_234276..234426_POSITIVE 299 148 108 147 106 smit:SMT_1635|3end_putative_N-acetyldiaminopimelate_deacetylase_1622636..1622786_POSITIVE 297 247 207 44 1 smit:SMT_2123|3end_hypothetical_protein_2008541..2008691_POSITIVE 297 250 207 138 80 smit:SMT_1538|3end_two-component_response_regulator_YvqC_1522669..1522819_NEGATIVE 296 230 184 77 24 smit:SMT_1776|3end_K07707_two-component_system,_AgrA_family,_response_regulator_AgrA_1751892..1752042_NEGATIVE 296 271 232 149 112 smit:SMT_486|3end_hypothetical_protein_421460..421610_NEGATIVE 294 236 193 75 14 smit:SMT_1812|3end_K07485_transposase_1787193..1787343_POSITIVE 293 240 194 51 6 smit:SMT_213|3end_leucyl-tRNA_synthetase_146167..146317_POSITIVE 287 250 201 54 11 smit:SMT_2068|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1974183..1974333_NEGATIVE 286 250 201 135 87 smit:SMT_1743|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1725058..1725208_NEGATIVE 284 51 15 133 95 smit:SMT_1265|3end_hypothetical_protein_1230872..1231022_NEGATIVE 281 154 114 143 107 smit:SMT_1254|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1220574..1220724_NEGATIVE 280 160 111 142 86 smit:SMT_813|3end_acetyltransferase,_GNAT_family_721867..722017_NEGATIVE 279 239 200 96 54 smit:SMT_756|3end_3-dehydroquinate_dehydratase_659907..660057_NEGATIVE 278 258 215 86 41 smit:SMT_292|3end_dTDP-glucose_4,6-dehydratase_235933..236083_POSITIVE