Origin IGS: aaaatgaaaatgaaaatgaacaaaatgcttgcgcttgcaagcgttttttgttatgataaaagagtcttcagggcagggtgtgattcccgaccggcggtgactttaactaggaaatgatcttttccttttatactttgttgacaagctttgcctaaccagaagttatgcctacagcttgtcgcctagtctaaaagaaaaatctctatttccttctctttaaagaagtccgcgagcgcaagctgatgtggtgagattccacaaccgacagtatagtctggatgggagaagacgaaagaatagctttgtctgttctgatagatttatagctaaactgtaaccgcttgcttgaatttccttaatagagtgagagggaacttttgggatataaaaagtgagaatagatagaaaatcctttctaacttcttctgattttatagaaaattggaggaacctgtt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aacaggttcctccaattttctataaaatcagaagaagttagaaaggattttctatctattctcactttttatatcccaaaagttccctctcactctattaaggaaattcaagcaagcggttacagtttagctataaatctatcagaacagacaaagctattctttcgtcttctcccatccagactatactgtcggttgtggaatctcaccacatcagcttgcgctcgcggacttctttaaagagaaggaaatagagatttttcttttagactaggcgacaagctgtaggcataacttctggttaggcaaagcttgtcaacaaagtataaaaggaaaagatcatttcctagttaaagtcaccgccggtcgggaatcacaccctgccctgaagactcttttatcataacaaaaaacgcttgcaagcgcaagcattttgttcattttcattttcatttt Mask Tandem Repeat Region ================================================ aaaatgaaaatgaaaatgaacaaaatgcttgcgcttgcaagcgttttttgttatgataaaagagtcttcagggcagggtgtgattcccgaccggcggtgactttaactaggaaatgatcttttccttttatactttgttgacaagctttgcctaaccagaagttatgcctacagcttgtcgcctagtctaaaagaaaaatctctatttccttctctttaaagaagtccgcgagcgcaagctgatgtggtgagattccacaaccgacagtatagtctggatgggagaagacgaaagaatagctttgtctgttctgatagatttatagctaaactgtaaccgcttgcttgaatttccttaatagagtgagagggaacttttgggatataaaaagtgagaatagatagaaaatcctttctaacttcttctgattttatagaaaattggaggaacctgtt Find is-nt database================================================ Query_seq: SMT_328:SMT_329|SMT_328:SMT_329:23S rRNA methyltransferase:hypothetical protein:->->:264924..265379 456 aaaatgaaaatgaaaatgaacaaaatgcttgcgcttgcaagcgttttttgttatgataaaagagtcttcagggcagggtgtgattcccgaccggcggtgactttaactaggaaatgatcttttccttttatactttgttgacaagctttgcctaaccagaagttatgcctacagcttgtcgcctagtctaaaagaaaaatctctatttccttctctttaaagaagtccgcgagcgcaagctgatgtggtgagattccacaaccgacagtatagtctggatgggagaagacgaaagaatagctttgtctgttctgatagatttatagctaaactgtaaccgcttgcttgaatttccttaatagagtgagagggaacttttgggatataaaaagtgagaatagatagaaaatcctttctaacttcttctgattttatagaaaattggaggaacctgtt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SMT_328:SMT_329|SMT_328:SMT_329:23S rRNA methyltransferase:hypothetical protein:->->:264924..265379 456 aaaatgaaaatgaaaatgaacaaaatgcttgcgcttgcaagcgttttttgttatgataaaagagtcttcagggcagggtgtgattcccgaccggcggtgactttaactaggaaatgatcttttccttttatactttgttgacaagctttgcctaaccagaagttatgcctacagcttgtcgcctagtctaaaagaaaaatctctatttccttctctttaaagaagtccgcgagcgcaagctgatgtggtgagattccacaaccgacagtatagtctggatgggagaagacgaaagaatagctttgtctgttctgatagatttatagctaaactgtaaccgcttgcttgaatttccttaatagagtgagagggaacttttgggatataaaaagtgagaatagatagaaaatcctttctaacttcttctgattttatagaaaattggaggaacctgtt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SMT_328:SMT_329|SMT_328:SMT_329:23S rRNA methyltransferase:hypothetical protein:->->:264924..265379 456 aaaatgaaaatgaaaatgaacaaaatgcttgcgcttgcaagcgttttttgttatgataaaagagtcttcagggcagggtgtgattcccgaccggcggtgactttaactaggaaatgatcttttccttttatactttgttgacaagctttgcctaaccagaagttatgcctacagcttgtcgcctagtctaaaagaaaaatctctatttccttctctttaaagaagtccgcgagcgcaagctgatgtggtgagattccacaaccgacagtatagtctggatgggagaagacgaaagaatagctttgtctgttctgatagatttatagctaaactgtaaccgcttgcttgaatttccttaatagagtgagagggaacttttgggatataaaaagtgagaatagatagaaaatcctttctaacttcttctgattttatagaaaattggaggaacctgtt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 1 Min: 242 Max: 405 Len: 164 Subject: UniRef90_Q97SA0 Cluster: Hypothetical protein; n=1; Streptococcus pneumoniae|Rep: Hypothetical protein - Streptococcus pneumoniae HSP 1 e-value: 4.0E-14 bit: 60.5 Len: 105 Query Start:242 Query End:346 Subject Strand: null Subject Start: 26 Subject End: 60 ................................................................................................................................................................................................................................................. S K R L Q F S Y K S I R T D K A I L S S S P I Q T I L S V V E S H H I .............................................................................................................. ................................................................................................................................................................................................................................................. S K W L Q S G Y K S I K T D K A I L S S S P I Q T I L S V V E S H H I .............................................................................................................. HSP 2 e-value: 4.0E-14 bit: 39.3 Len: 72 Query Start:334 Query End:405 Subject Strand: null Subject Start: 6 Subject End: 33 ............................................................................................................................................................................................................................................................................................................................................. L S I L T F Y I P K V P S H S I K E I Q A - - - - S G Y ................................................... ............................................................................................................................................................................................................................................................................................................................................. L S I L T F Y I P K V P S Y S I K E K Q S K W L Q S G Y ................................................... aaaatgaaaatgaaaatgaacaaaatgcttgcgcttgcaagcgttttttgttatgataaaagagtcttcagggcagggtgtgattcccgaccggcggtgactttaactaggaaatgatcttttccttttatactttgttgacaagctttgcctaaccagaagttatgcctacagcttgtcgcctagtctaaaagaaaaatctctatttccttctctttaaagaagtccgcgagcgcaagctnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnaaaatcctttctaacttcttctgattttatagaaaattggaggaacctgtt Predict ORF larger than 30AA ================================================ Protein_Len: 37 Strand: + Start: 4 End: 114 ... M K M K M N K M L A L A S V F C Y D K R V F R A G C D S R P A V T L T R K ...................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 30 Strand: + Start: 245 End: 334 .................................................................................................................................................................................................................................................... M V R F H N R Q Y S L D G R R R K N S F V C S D R F I A K L .......................................................................................................................... Protein_Len: 60 Strand: - Start: 220 End: 435 ........................................................................................................................................................................................................................... W L R C Y L R S P L L R F F L K T Q E S L N I A L S Y G S A Q I E K I S H S P V K P I Y F T L I S L F D K R V E E S K M ..................... Protein_Len: 37 Strand: - Start: 206 End: 316 ............................................................................................................................................................................................................. K R R K L S T R S R L S I H H S E V V S L I T Q I P S S S L I A K D T R M ............................................................................................................................................ Protein_Len: 45 Strand: - Start: 186 End: 320 ......................................................................................................................................................................................... D L L F I E I E K E K F F D A L A L Q H P S I G C G V T Y D P H S F V F S Y S Q R N Q Y M ........................................................................................................................................ aaaatgaaaatgaaaatgaacaaaatgcttgcgcttgcaagcgttttttgttatgataaaagagtcttcagggcagggtgtgattcccgaccggcggtgactttaactaggaaatgatcttttccttttatactttgttgacaagctttgcctaaccagaagttatgcctacagcttgtcgcctagtctaaaagaaaaatctctatttccttctctttaaagaagtccgcgagcgcaagctnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnaaaatcctttctaacttcttctgattttatagaaaattggaggaacctgtt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 82 Start: 28 End: 57 ...........................CTTGCGCTTGCAAGCGTTTTTTGTTATGAT............................................................................................................................................................................................................................................................................................................................................................................................................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aaaatgaaaatgaaaatgaacaaaatgcttgcgcttgcaagcgttttttgttatgataaaagagtcttcagggcagggtgtgattcccgaccggcggtgactttaactaggaaatgatcttttccttttatactttgttgacaagctttgcctaaccagaagttatgcctacagcttgtcgcctagtctaaaagaaaaatctctatttccttctctttaaagaagtccgcgagcgcaagctnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnaaaatcctttctaacttcttctgattttatagaaaattggaggaacctgtt Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 76 HP_score: -5.5 Tail_Score: -4.89562 Start: 26 End: 43 Full_Region: tatcataacaaaaaa cgcttgc aagc gcaagca ttttgttcattttca .........................cgcttgcaagcgcaagca............................................................................................................................................................................................................................................................................................................................................................................................................................. TransTerm Strand: - Conf: 74 HP_score: -9.0 Tail_Score: -3.5211 Start: 27 End: 42 Full_Region: atcataacaaaaaac gcttgc aagc gcaagc attttgttcattttc ..........................gcttgcaagcgcaagc.............................................................................................................................................................................................................................................................................................................................................................................................................................. TransTerm Strand: + Conf: 64 HP_score: -3.3 Tail_Score: -4.67256 Start: 110 End: 125 Full_Region: CGGTGACTTTAACTA GGAAA TGATCT TTTCC TTTTATACTTTGTTG .............................................................................................................GGAAATGATCTTTTCC........................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: - Conf: 44 HP_score: -3.3 Tail_Score: -2.73364 Start: 110 End: 125 Full_Region: caacaaagtataaaa ggaaa agatca tttcc tagttaaagtcaccg .............................................................................................................ggaaaagatcatttcc........................................................................................................................................................................................................................................................................................................................................... Find igs database================================================ Query_seq: SMT_328:SMT_329|SMT_328:SMT_329:23S rRNA methyltransferase:hypothetical protein:->->:264924..265379 456 aaaatgaaaatgaaaatgaacaaaatgcttgcgcttgcaagcgttttttgttatgataaaagagtcttcagggcagggtgtgattcccgaccggcggtgactttaactaggaaatgatcttttccttttatactttgttgacaagctttgcctaaccagaagttatgcctacagcttgtcgcctagtctaaaagaaaaatctctatttccttctctttaaagaagtccgcgagcgcaagctnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnaaaatcctttctaacttcttctgattttatagaaaattggaggaacctgtt Intra-Species Hit: Count: 1 Min: 1 Max: 456 Len: 456 Subject: smit_SMT_328_SMT_329|23S rRNA methyltransferase:hypothetical protein|POSITIVE:POSITIVE|[264924,265379]|456 HSP 1 e-value: 1.0E-134 bit: 478.0 Len: 241 Query Start:1 Query End:241 Subject Strand: POSITIVE Subject Start: 1 Subject End: 241 aaaatgaaaatgaaaatgaacaaaatgcttgcgcttgcaagcgttttttgttatgataaaagagtcttcagggcagggtgtgattcccgaccggcggtgactttaactaggaaatgatcttttccttttatactttgttgacaagctttgcctaaccagaagttatgcctacagcttgtcgcctagtctaaaagaaaaatctctatttccttctctttaaagaagtccgcgagcgcaagct....................................................................................................................................................................................................................... aaaatgaaaatgaaaatgaacaaaatgcttgcgcttgcaagcgttttttgttatgataaaagagtcttcagggcagggtgtgattcccgaccggcggtgactttaactaggaaatgatcttttccttttatactttgttgacaagctttgcctaaccagaagttatgcctacagcttgtcgcctagtctaaaagaaaaatctctatttccttctctttaaagaagtccgcgagcgcaagct....................................................................................................................................................................................................................... HSP 2 e-value: 2.0E-20 bit: 101.0 Len: 51 Query Start:406 Query End:456 Subject Strand: POSITIVE Subject Start: 406 Subject End: 456 .....................................................................................................................................................................................................................................................................................................................................................................................................................aaaatcctttctaacttcttctgattttatagaaaattggaggaacctgtt .....................................................................................................................................................................................................................................................................................................................................................................................................................aaaatcctttctaacttcttctgattttatagaaaattggaggaacctgtt Inter-species Hit: Count: 3 Min: 1 Max: 456 Len: 456 Subject: NC_008533_SPD_0436_SPD_0437|TrmH family RNA methyltransferase:hypothetical protein|POSITIVE:POSITIVE|[440916,441373]|458 HSP 1 e-value: 1.0E-134 bit: 478.0 Len: 241 Query Start:1 Query End:241 Subject Strand: POSITIVE Subject Start: 1 Subject End: 241 aaaatgaaaatgaaaatgaacaaaatgcttgcgcttgcaagcgttttttgttatgataaaagagtcttcagggcagggtgtgattcccgaccggcggtgactttaactaggaaatgatcttttccttttatactttgttgacaagctttgcctaaccagaagttatgcctacagcttgtcgcctagtctaaaagaaaaatctctatttccttctctttaaagaagtccgcgagcgcaagct....................................................................................................................................................................................................................... aaaatgaaaatgaaaatgaacaaaatgcttgcgcttgcaagcgttttttgttatgataaaagagtcttcagggcagggtgtgattcccgaccggcggtgactttaactaggaaatgatcttttccttttatactttgttgacaagctttgcctaaccagaagttatgcctacagcttgtcgcctagtctaaaagaaaaatctctatttccttctctttaaagaagtccgcgagcgcaagct....................................................................................................................................................................................................................... HSP 2 e-value: 8.0E-17 bit: 89.7 Len: 49 Query Start:408 Query End:456 Subject Strand: POSITIVE Subject Start: 410 Subject End: 458 .......................................................................................................................................................................................................................................................................................................................................................................................................................aatcctttctaacttcttctgattttatagaaaattggaggaacctgtt .......................................................................................................................................................................................................................................................................................................................................................................................................................aatcctttccaacttcttctgattttatagaaaattggaggaacctgtt Subject: NC_003098_spr0432_spr0433|rRNA methylase:hypothetical protein|POSITIVE:POSITIVE|[433386,433828]|443 HSP 1 e-value: 1.0E-134 bit: 478.0 Len: 241 Query Start:1 Query End:241 Subject Strand: POSITIVE Subject Start: 1 Subject End: 241 aaaatgaaaatgaaaatgaacaaaatgcttgcgcttgcaagcgttttttgttatgataaaagagtcttcagggcagggtgtgattcccgaccggcggtgactttaactaggaaatgatcttttccttttatactttgttgacaagctttgcctaaccagaagttatgcctacagcttgtcgcctagtctaaaagaaaaatctctatttccttctctttaaagaagtccgcgagcgcaagct....................................................................................................................................................................................................................... aaaatgaaaatgaaaatgaacaaaatgcttgcgcttgcaagcgttttttgttatgataaaagagtcttcagggcagggtgtgattcccgaccggcggtgactttaactaggaaatgatcttttccttttatactttgttgacaagctttgcctaaccagaagttatgcctacagcttgtcgcctagtctaaaagaaaaatctctatttccttctctttaaagaagtccgcgagcgcaagct....................................................................................................................................................................................................................... HSP 2 e-value: 7.0E-8 bit: 60.0 Len: 34 Query Start:408 Query End:441 Subject Strand: POSITIVE Subject Start: 410 Subject End: 443 .......................................................................................................................................................................................................................................................................................................................................................................................................................aatcctttctaacttcttctgattttatagaaaa............... .......................................................................................................................................................................................................................................................................................................................................................................................................................aatcctttccaacttcttctgattttatagaaaa............... Subject: NC_003028_SP_0486_SP_0487|RNA methyltransferase, TrmH family:hypothetical protein|POSITIVE:NEGATIVE|[467099,467294]|196 HSP 1 e-value: 1.0E-102 bit: 373.0 Len: 196 Query Start:7 Query End:202 Subject Strand: POSITIVE Subject Start: 1 Subject End: 196 ......aaaatgaaaatgaacaaaatgcttgcgcttgcaagcgttttttgttatgataaaagagtcttcagggcagggtgtgattcccgaccggcggtgactttaactaggaaatgatcttttccttttatactttgttgacaagctttgcctaaccagaagttatgcctacagcttgtcgcctagtctaaaagaaaaatct.............................................................................................................................................................................................................................................................. ......aaaatgaaaatgaacaaaatgcttgcgcttgcaagcgttttttgttatgataaaagagtcttcagggcagggtgaaattcccgaccggcggtgactttaactaggaaatgatcttttccttttatactttgttgacaagctttgcctaaccagaagttatgcctacagcttgtcgcctagtctaaaagaaaaatct.............................................................................................................................................................................................................................................................. Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAAAUGAAAAUGAAAAUGAACAAAAUGCUUGCGCUUGCAAGCGUUUUUUGUUAUGAUAAAAGAGUCUUCAGGGCAGGGUGUGAUUCCCGACCGGCGGUGACUUUAACUAGGAAAUGAUCUUUUCCUUUUAUACUUUGUUGACAAGCUUUGCCUAACCAGAAGUUAUGCCUACAGCUUGUCGCCUAGUCUAAAAGAAAAAUCUCUAUUUCCUUCUCUUUAAAGAAGUCCGCGAGCGCAAGCUGAUGUGGUGAGAUUCCACAACCGACAGUAUAGUCUGGAUGGGAGAAGACGAAAGAAUAGCUUUGUCUGUUCUGAUAGAUUUAUAGCUAAACUGUAACCGCUUGCUUGAAUUUCCUUAAUAGAGUGAGAGGGAACUUUUGGGAUAUAAAAAGUGAGAAUAGAUAGAAAAUCCUUUCUAACUUCUUCUGAUUUUAUAGAAAAUUGGAGGAACCUGUU ..........................((((((((((((..((.(((((((....((....((((((.((.((...(((.......)))..))...)).))))))....(((((((((..((((.((((((.(((..((.((((((((..((.((((.....)))).))....))))))))))..))).)))))).))))..)).)))))))))....)))))))))..))))))))))))...(((((.......)))))...(((((...(((.((((.((((.....(....)......((((((((((((.((...((((((.(((((..((..((.((..(((..(((.....))).)))..)).))..)).)))))..))))))..)).))))))))))))...)))).)))))))(((((..(((((....)))))..)))))..))))) (-134.00) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AACAGGUUCCUCCAAUUUUCUAUAAAAUCAGAAGAAGUUAGAAAGGAUUUUCUAUCUAUUCUCACUUUUUAUAUCCCAAAAGUUCCCUCUCACUCUAUUAAGGAAAUUCAAGCAAGCGGUUACAGUUUAGCUAUAAAUCUAUCAGAACAGACAAAGCUAUUCUUUCGUCUUCUCCCAUCCAGACUAUACUGUCGGUUGUGGAAUCUCACCACAUCAGCUUGCGCUCGCGGACUUCUUUAAAGAGAAGGAAAUAGAGAUUUUUCUUUUAGACUAGGCGACAAGCUGUAGGCAUAACUUCUGGUUAGGCAAAGCUUGUCAACAAAGUAUAAAAGGAAAAGAUCAUUUCCUAGUUAAAGUCACCGCCGGUCGGGAAUCACACCCUGCCCUGAAGACUCUUUUAUCAUAACAAAAAACGCUUGCAAGCGCAAGCAUUUUGUUCAUUUUCAUUUUCAUUUU ((((((.......((((((((..........(((((((.((((.((((.....)))).)))).)))))))..........(((........)))......))))))))...(((((((.....((..(((((.....(((........)))...)))))..))...((((((.........(((......)))(((.(((((.(((((((.....(((....))).((((....((((((.....(((((((.((..(((((((((((.(((.(..((((((((....((.((((.....)))).))..))))))))..)..))).)))))))))))..)))))))))..))))))...)))).))).)))).)).)))...)))..))))))....................)))))))..((....))..)))))).................. (-114.30) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 377 250 201 218 159 smit:SMT_749|5end_shikimate_kinase_653971..654201_NEGATIVE 335 265 224 173 128 smit:SMT_169|5end_30S_ribosomal_protein_S10_114515..114745_POSITIVE 333 97 62 168 122 smit:SMT_2052|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1961591..1961821_NEGATIVE 325 180 131 209 162 smit:SMT_1694|5end_GTPase_1676674..1676904_POSITIVE 316 80 34 65 7 smit:SMT_1638|5end_UTP-glucose-1-phosphate_uridylyltransferase_1624781..1625011_NEGATIVE 313 315 275 53 14 smit:SMT_1823|5end_GTP-binding_protein_1794982..1795212_POSITIVE 310 110 62 177 134 smit:SMT_1836|5end_cytidine/deoxycytidylate_deaminase_family_protein_1804498..1804728_NEGATIVE 310 110 62 177 134 smit:SMT_1674|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1660501..1660731_NEGATIVE 306 265 222 227 189 smit:SMT_196|5end_30S_ribosomal_protein_S11_127260..127490_POSITIVE 302 373 332 50 1 smit:SMT_315|5end_acetyl-coenzyme_A_carboxylase_carboxyl_transferase_subunit_beta_255681..255911_POSITIVE 296 326 284 93 33 smit:SMT_1284|5end_predicted_amidophosphoribosyltransferase_1246962..1247192_POSITIVE 295 90 42 79 37 smit:SMT_1517|5end_hypothetical_protein_predicted_by_Glimmer/Critica_1498313..1498543_POSITIVE 295 253 229 231 202 smit:SMT_775|5end_alpha-acetolactate_decarboxylase_685972..686202_NEGATIVE 293 101 61 49 10 smit:SMT_1367|5end_K02237_competence_protein_ComEA_1347522..1347752_NEGATIVE 293 104 62 67 7 smit:SMT_913|5end_putative_sucrose-specific_PTS_permease,_enzyme_II_865809..866039_NEGATIVE 293 264 221 209 147 smit:SMT_392|5end_acetyltransferase,_GNAT_family_323616..323846_POSITIVE 290 249 204 153 100 smit:SMT_573|5end_1-phosphofructokinase_490976..491206_POSITIVE 290 250 221 52 23 smit:SMT_186|5end_50S_ribosomal_protein_L18_122623..122853_POSITIVE 289 266 221 59 2 smit:SMT_1730|5end_geranyltranstransferase_1714775..1715005_POSITIVE 289 109 70 56 3 smit:SMT_1703|5end_recombination_protein_RecR_1686660..1686890_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 333 105 61 61 11 smit:SMT_1559|3end_6-phosphofructokinase_1544614..1544764_NEGATIVE 323 109 70 44 7 smit:SMT_83|3end_K07707_two-component_system,_AgrA_family,_response_regulator_AgrA_14543..14693_NEGATIVE 319 255 224 79 42 smit:SMT_1477|3end_K02077_zinc/manganese_transport_system_substrate-binding_protein_1459447..1459597_NEGATIVE 317 255 229 35 4 smit:SMT_1872|3end_K03217_preprotein_translocase_YidC_subunit_1830883..1831033_NEGATIVE 313 315 275 50 11 smit:SMT_1822|3end_hypothetical_protein_1795059..1795209_POSITIVE 312 257 211 71 24 smit:SMT_1484|3end_exonuclease_RexA_1464256..1464406_NEGATIVE 311 100 71 50 1 smit:SMT_2057|3end_cell_division_protein_FtsH_1966897..1967047_NEGATIVE 296 326 284 96 36 smit:SMT_1283|3end_K02240_competence_protein_ComFA_1247045..1247195_POSITIVE 294 52 19 72 39 smit:SMT_328|3end_23S_rRNA_methyltransferase_264863..265013_POSITIVE 290 255 224 76 39 smit:SMT_799|3end_hypothetical_protein_707296..707446_NEGATIVE 289 266 221 62 5 smit:SMT_1729|3end_exodeoxyribonuclease_VII_small_subunit_1714858..1715008_POSITIVE 287 279 235 145 102 smit:SMT_1266|3end_predicted_transcriptional_regulator_1231774..1231924_POSITIVE 286 189 144 59 19 smit:SMT_1689|3end_PTS_enzyme,_maltose-and_glucose-specific,_factor_II_homologue_1673139..1673289_POSITIVE 284 192 151 145 104 smit:SMT_833|3end_hypothetical_protein_predicted_by_Glimmer/Critica_775183..775333_NEGATIVE 283 450 411 57 19 smit:SMT_672|3end_hypothetical_protein_580899..581049_POSITIVE 282 55 11 95 47 smit:SMT_1786|3end_phenylalanyl-tRNA_synthetase_beta_chain_1763235..1763385_NEGATIVE 279 255 224 79 41 smit:SMT_2109|3end_hypothetical_protein_predicted_by_Glimmer/Critica_1999061..1999211_NEGATIVE 279 255 224 79 41 smit:SMT_1809|3end_MutT/NUDIX_family_protein_1785134..1785284_POSITIVE 279 255 224 79 41 smit:SMT_1180|3end_putative_tagatose-6-phosphate_aldose/ketose_isomerase_1138036..1138186_NEGATIVE 276 218 173 132 86 smit:SMT_2087|3end_cadmium_efflux_system_accessory_protein_1984108..1984258_POSITIVE